CN107548990A - A kind of cultural method for improving duckweed content of starch and application - Google Patents
A kind of cultural method for improving duckweed content of starch and application Download PDFInfo
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Abstract
The present invention relates to aquatic energy-source plant culture field, and in particular to a kind of cultural method for improving duckweed content of starch and application.The invention provides a kind of cultural method for improving duckweed content of starch, it includes following step：Duckweed is inoculated in water surface, then uniconazole P solution is sprayed to the duckweed surface being grown in water body, is then passed through CO2, obtain target product duckweed.Methods described is not limited by water body, either in the water body of relatively eutrophic water body or relative oligotrophic, the content of starch of duckweed can be increased substantially, the duckweed of large-scale culture high content of starch especially in the water body being contaminated, the efficient removal of nitrogen phosphorus in sewage is also achieved while Rapid Accumulation high content of starch, on the one hand production cost is reduced, obtain the duckweed of high content of starch, on the other hand the water body nitrogen and phosphorus pollution problem faced at present can effectively be alleviated, and methods described is simple to operate, cost is cheap, easy popularization and application.
Description
Technical field
The present invention relates to aquatic energy-source plant culture field, and in particular to a kind of cultural method for improving duckweed content of starch
And application.
Background technology
With the mankind, to the growing of energy demand, fossil energy, increasingly deficient and ecological problem is on the rise, can be again
The exploitation of the raw energy is of increased attention.Biological liquid fuel due to the popularity and recyclability of its raw material, into
For the focus of whole world research, wherein alcohol fuel as the currently the only substitute energy source for petroleum for being suitable for available engine system,
It is the most extensive using scale.At present, the primary raw material of alcohol fuel includes the terrestrial plants such as corn, sugarcane, cassava, sweet potato, raw
It is too high to produce cost, in order to reduce its production cost, it is necessary to find cheap, sustainable vegetable material as raw materials for production, water
Raw energy-source plant with its non-grain attribute, be not take up the advantage in arable land, become one of desirable feedstock.
Aquatic energy-source plant duckweed (Duckweed) is the general designation of Lemnaceae (Lemnaceae) plant, shares 5 and belongs to 37
Kind, it is minimum, simplest flowering plant in the world, its is widely distributed, in addition to most cold area, has in the world point
Cloth.The speed of growth of duckweed is exceedingly fast, and its biomass can be double in 16-48 hours under ideal conditions, more high than most
Plant growth is fast.Meanwhile duckweed can grow in sewage, the pollutants such as nitrogen phosphorus therein are absorbed, it is grown simultaneously using waste water
The characteristic of high-quality biological matter raw material, and the advantage that pollution control and bioenergy are organically combined are produced, is just being scientist
Paid attention to.Under optimal nutrition supply, illumination and water temperature condition, the annual dry matter production of duckweed is up to 55 tons/hectare.
Reached its maturity currently with the technique of duckweed production ethanol, but the technique exist most important limitation because
The content of starch of element, i.e. duckweed is relatively low, limits the concentration of fermentation gained ethanol to a certain extent.Therefore large-scale culture goes out height
The duckweed of content starch is applied to significant with popularization.The method of culture high content of starch duckweed is mainly at present：
(1) under the conditions of relative oligotrophic, i.e. N, P concentration be below cultivating duckweed under the conditions of 0.1ppm 8 days (Xu J., Cui W.,
Cheng J.J.,Stomp A.M.Production of high-starch duckweed and its conversion to
bioethanol.Biosystems Engineering.2011,110(2):67-72.), this method can make duckweed content of starch
Up to 29.8%, but there is following deficiency for this method：1. requiring high to water body, this method requirement is in relative oligotrophic
Duckweed is cultivated in water body, with the increasingly exacerbation of water pollution, natural water is extremely difficult to the standard, greatly limit this method
Application and popularization；2. duckweed is cultivated in relative oligotrophic water body, although improving the content of starch of duckweed, serious suppression
The growth of duckweed has been made, so as to reduce the yield of biomass of duckweed, has caused starch yield not significantly improve；(2) normal
Under nutritional condition, i.e. certain density uniconazole P hydroponics duckweed is sprayed in the Hoagland nutrient solutions of 1/6 concentration 10 days
(LiuY.,Fang Y.,Huang M.,et al.Uniconazole-induced starch accumulation in the
bioenergy crop duckweed(Landoltiapunctata)II:transcriptome alterations of
pathways involved in carbohydrate metabolism and endogenous hormone
crosstalk.Biotechnology for Biofuels.2015,8:64), this method can be up to duckweed content of starch
To 48.01%, the advantage of this method is not limited by water body, and duckweed can be cultivated in the water body of normal nutrition, both can be with
Duckweed content of starch is improved, and does not influence duckweed growth, but this method is disadvantageous in that duckweed content of starch is less than 50%,
It is unfavorable for the accumulation of alcohol concentration during industrial fermentation.
The content of the invention
Therefore, the present invention be directed to cultivate the deficiency of high content of starch duckweed at present, there is provided one kind improves duckweed starch and contained
The cultural method of amount, this method can increase substantially duckweed content of starch, be advantageously implemented the extensive of high content of starch duckweed
Culture.
The invention provides a kind of cultural method for improving duckweed content of starch, it is that duckweed is inoculated in into water surface,
Uniconazole P solution is sprayed to the duckweed surface being grown in water body, is then passed through CO2, obtain target product duckweed.
Specifically, the present invention proposes following technical scheme.
The invention provides a kind of cultural method for improving duckweed content of starch, following step is included：Duckweed is inoculated in
Water surface, uniconazole P solution then is sprayed to the duckweed surface being grown in water body, is then passed through CO2, obtain target product
Duckweed.
Preferably, for described cultural method, wherein, the CO2Concentration be 500-3000ppm, preferably 650-
1200ppm。
Preferably, for described cultural method, wherein, the concentration of the uniconazole P is 100~1000mg/L, is preferably
500~800mg/L.
Preferably, for described cultural method, wherein, the fountain height of the uniconazole P solution is every square metre of duckweed
150~300mL.
Preferably, for described cultural method, wherein, it is being passed through CO2After cultivated, incubation time >=7 day, preferably
For 7-10 days.
Preferably, for described cultural method, wherein, the culture is to be cultivated under light illumination, the illumination bar
Part is full exposure.
Preferably, for described cultural method, wherein, the water body is relative eutrophic water body or relative oligotrophic water
Body.
Preferably, for described cultural method, wherein, duckweed is inoculated in water body according to 80%-100% coverage rate
Surface.
A kind of cultural method for improving duckweed content of starch provided by the invention, it is to cultivate to float in the water body of pollution
Duckweed.
Preferably, for described cultural method, its application in purifying contaminated water body.
Beneficial effect acquired by the present invention is：
1. the invention provides a kind of method for improving duckweed content of starch, this method is not limited by water condition, nothing
By being in the water body of relatively eutrophic water body or relative oligotrophic, the content of starch of duckweed can be increased substantially, is had
Beneficial to the large-scale plantation and application for promoting duckweed.
2. the method for the invention is by spraying uniconazole P solution on duckweed surface and adding CO2Technological means, training
After supporting 7 days, the content of starch of duckweed is 75.16%, is cultivated in 7-10 days, and duckweed content of starch is cultivated 10 days without significantly improving
Afterwards, duckweed content of starch is up to 75.88%, and starch rate of growth has reached 56~350%, starch maximum output equivalent to
2.05 tons/mu/year.
3. the method for the invention is taken in duckweed surface sprinkling uniconazole P solution and adds CO2Technological means, duckweed
Can in different water bodys fast-growth, the duckweed of large-scale culture high content of starch especially in the water body being contaminated, fast
The efficient removal of nitrogen phosphorus in sewage is also achieved while speed accumulation high content of starch, the accumulation of duckweed content of starch is in time
30% is shortened, on the one hand can reduce production cost, obtains the duckweed of high content of starch, on the other hand, can effectively be alleviated at present
The water body nitrogen and phosphorus pollution problem faced.
4. the method for the invention is simple to operate, cost is cheap, easy popularization and application.
Brief description of the drawings
Fig. 1 is that duckweed described in embodiment one sprays uniconazole P solution in relative oligotrophic water body and is passed through CO2Starch afterwards
Content with incubation time changing trend diagram；
Fig. 2 is that duckweed described in embodiment two sprays uniconazole P solution in relative eutrophic water body and is passed through CO2Starch afterwards
Content with incubation time changing trend diagram；
Fig. 3 is that duckweed described in embodiment three sprays uniconazole P solution in relative oligotrophic water body and is passed through CO2Form sediment afterwards
Powder content with incubation time changing trend diagram；
Fig. 4 is that duckweed described in example IV sprays uniconazole P solution in relative eutrophic water body and is passed through CO2Starch afterwards
Content with incubation time changing trend diagram；
Fig. 5 is that duckweed described in embodiment five sprays uniconazole P solution in relative oligotrophic water body and is passed through CO2Starch afterwards
Content with incubation time changing trend diagram；
Fig. 6 is that duckweed described in embodiment six sprays uniconazole P solution in relative eutrophic water body and is passed through CO2Starch afterwards
Content with incubation time changing trend diagram；
Fig. 7 is that duckweed described in embodiment seven sprays uniconazole P solution in relative oligotrophic water body and is passed through CO2Starch afterwards
Content with incubation time changing trend diagram；
Fig. 8 is that duckweed described in embodiment eight sprays uniconazole P solution in relative eutrophic water body and is passed through CO2Starch afterwards
Content with incubation time changing trend diagram；
Fig. 9 is that duckweed described in embodiment nine sprays uniconazole P solution in relative oligotrophic water body and is passed through CO2Starch afterwards
Content with incubation time changing trend diagram；
Figure 10 is that duckweed described in embodiment 10 sprays uniconazole P solution in relative eutrophic water body and is passed through CO2Starch afterwards
Content with incubation time changing trend diagram；
Figure 11 is that duckweed described in comparative example one is passed through CO in relative oligotrophic water body2Content of starch is with incubation time afterwards
Changing trend diagram；
Figure 12 is that duckweed is passed through CO in relative eutrophic water body described in comparative example two2Content of starch is with incubation time afterwards
Changing trend diagram；
Figure 13 is that duckweed described in comparative example three is passed through CO in relative oligotrophic water body2Content of starch is with incubation time afterwards
Changing trend diagram；
Figure 14 is that duckweed is passed through CO in relative eutrophic water body described in comparative example four2Content of starch is with incubation time afterwards
Changing trend diagram；
When Figure 15 is that duckweed described in comparative example five sprays that content of starch is with culture after uniconazole P in relative oligotrophic water body
Between the tendency chart that changes；
When Figure 16 is that duckweed described in comparative example six sprays that content of starch is with culture after uniconazole P in relative eutrophic water body
Between the tendency chart that changes.
Embodiment
Term refers to Water phosphorus content " with respect to oligotrophic water body " and is less than 0.1ppm (mg/L), more than this mark rule definition
For relative eutrophic water body.
As described above, the invention provides a kind of cultural method for improving duckweed content of starch, it includes following step：Will
Duckweed is inoculated in water surface, then sprays uniconazole P solution to the duckweed surface being grown in water body, is then passed through CO2, obtain
To target product duckweed.In order to save time cost, after duckweed is inoculated in water body, uniconazole P solution is sprayed immediately, is being sprayed
After uniconazole P solution, CO is passed through immediately2。
The target product duckweed, refers to the duckweed of high content of starch, and the high content of starch refers to that content of starch is more than
50%, especially content of starch are more than 65%.
Wherein, the CO2Concentration be 500-3000ppm, preferably 650-1200ppm, the CO2It is passed through using continuous
Or interval is passed through.
Wherein, the concentration of the uniconazole P is 100~1000mg/L, preferably 500~800mg/L, and the uniconazole P is molten
The fountain height of liquid is every square metre of 150~300mL of duckweed.
The uniconazole P is plant growth regulator, has control nutrient growth, suppresses cell elongation, shortens internode, be short
Change plant, promote lateral bud growth and flower_bud formation, promote the effect of resistance.
It is described to be passed through CO2Effect be to improve duckweed photosynthetic efficiency, strengthen the product of duckweed triose phosphate and starch
It is tired to improve duckweed growth speed simultaneously.
Wherein, it is being passed through CO2After cultivated, incubation time >=7 day, due to after duckweed growth 7 days, starch in duckweed
Content tends towards stability, in order to save time cost, after duckweed is cultivated 7-10 days, you can harvest.
Wherein, the culture is to be cultivated under light illumination, and the illumination condition is full exposure, the culture of the full exposure
Condition：Temperature is 25 DEG C, and intensity of illumination is 130 μM of photos m-2s-1, periodicity of illumination 24h：0h.
The water body is relative eutrophic water body or relative oligotrophic water body, i.e., described water body is distilled water or 1/5 concentration
Hoagland solution.
Disclosed in CN103609304B using uniconazole P or paclobutrazol sprinkling duckweed, it is possible to increase the starch of duckweed contains
Amount, but in a particular embodiment, after spraying uniconazole P or paclobutrazol, duckweed content of starch illustrates individually below 50%
After uniconazole P or paclobutrazol sprinkling duckweed, duckweed content of starch increases DeGrain.
Duckweed below by embodiment and comparative example and with reference to accompanying drawing to Rapid Accumulation high content of starch of the present invention
Cultural method is described further.Reagent and testing equipment used in embodiment and comparative example, non-specified otherwise, be routine
Reagent and experimental facilities, analysis method used in embodiment and comparative example is using conventional analysis method.
In following embodiments and comparative example, few (abbreviation L.punctata of root duckweed Landoltia.punctata 0202
0202) collection is stored in Chinese Academy of Sciences's Chengdu biological study from Sichuan Province China province Chengdu Xinjin County, the duckweed of above-mentioned kind
Duckweed germplasm database.(Rutgers Duckweed Stock Cooperative,RDSC,http://
Www.ruduckweed.org/), the duckweed is documented in Publication No. CN103947529A《Duckweed starch and thick is improved simultaneously
The method of protein yield and sewerage nitrogen and phosphor clearance》In.
In following embodiments and comparative example：
The assay method of duckweed fresh weight can be found in Alicja Piotrowska, Andrzej Bajguz et
al.Jasmonic acid as modulator of lead toxicity in aquatic plant Wolffia
(2009) 507-513 of arrhiza (Lemnaceae) .Environmental and Experimental Botany 66, specifically
For：The duckweed for cultivating certain time is taken out, is cleaned 3 times with distilled water, is then placed 5 minutes on filter paper, centrifugal dehydration is (every
Secondary 5min), weigh.
The assay method of duckweed dry weight：By the duckweed of harvest with centrifugal dehydration (each 5min), 60 DEG C of baking is subsequently placed in
Constant weight is dried in case, is weighed.
The assay method of duckweed content of starch can be found in Zhang L., Chen Q., Jin Y., et al.Energy-
saving direct ethanol production from viscosity reduction mash of sweet
potato at very high gravity(VHG).Fuel Processing Technology.2010,91(12):1845-
1850.Specifically assay method is：Dry duckweed is ground into the powder of 100 mesh or so, weighs 0.03~0.06g duckweed dry powder
It is placed in 250mL ground conical flasks, adds 30mL 6mol/L HCl solution and 100mL distilled water, load onto condenser pipe, put boiling water
Flow back 2h in bath.Backflow finishes, and immediately with flowing water cooling, after duckweed sample hydrolyzate is cooled to room temperature, adds NaOH and adjusts
The pH value for saving hydrolyzate is 7.0.Then 20mL 20wt% acetic acid lead solutions are added, 10min is placed after shaking up, is transferred to 500mL
In volumetric flask, add distilled water to be settled to 500mL, filter, discard primary filtrate, collection 5mL filtrates are crossed the good anti-phase C18 of pre-activate and consolidated
Mutually extraction pillar, discards 1 initial~2mL, collects 3~4mL below, then with 0.22 μm of water system membrane filtration.Utilize
Glucose content in HPLC measure filtrates, according to content of starch=glucose content/1.1, calculates the content of starch of duckweed.
In following each embodiments, the compound method of various solution is as follows：
The compound method of Hoagland nutrient solutions is as follows：(1) with distilled water according to respectively being tried in the mother liquor formula described in following table
The concentration of agent prepares six kinds of mother liquors of A, B, C, D, E, F, wherein, when preparing mother liquor A, first with 6M HCl solution by Ca (NO3)2·
4H2O、KNO3、KH2PO4Dissolving, then add distilled water and be configured to aimed concn；When preparing mother liquor D, first with 6M KOH solution
EDTA is dissolved, distilled water is then added and is configured to aimed concn.(2) according in every liter of Hoagland nutrient solution described in following table
The addition of various mother liquors prepares Hoagland nutrient solutions with distilled water, and Hoagland nutrient solutions are adjusted by HCl and NaOH
PH value is 5.0.In the Hoagland nutrient solutions prepared, the concentration of N element is 349.73mg/L, and the concentration of P element is
154.89mg/L。
The compound method of 1/5 concentration Hoagland nutrient solutions：The Hoagland nutrient solutions of certain volume are taken to add 4 times of volumes
Distilled water, it is well mixed, that is, obtains 1/5 concentration Hoagland nutrient solutions.
The pure uniconazole P powder (be purchased from Sigma companies) of a certain amount of analysis is weighed, after methanol dissolves, constant volume is configured to 10 ×
Using the uniconazole P mother liquor of concentration, i.e. concentration is 1.0~10.0g/L, then with 10 times of distilled water diluting, is configured to final concentration of
100~1000mg/L uniconazole P solution, while the final concentration of 10% (volume of methanol：Volume).
Following embodiments and comparative example are synchronously carried out.
Embodiment one sprays uniconazole P and adds CO2Duckweed is cultivated in distilled water (with respect to oligotrophic condition)
It is 54cm in surface area2Culture vessel in add 500mL distilled water, take initial starch content for 6.52wt%,
Initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (coverage rate is about 100%), so
(matched somebody with somebody immediately to the duckweed surface uniconazole P solution that uniformly 1~2mL of sprinkling concentration is 500mg/L with analytically pure uniconazole P afterwards
System), after sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, illumination is strong
Spend for 130 μM of photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2Concentration be maintained at
Near 650ppm.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day, the 5th day, the 7th day
With the 10th day harvest duckweed sample, cleaned 3 times, then placed 5 minutes on filter paper, centrifugal dehydration 5 minutes with distilled water, will after
Fresh duckweed is placed in 60 DEG C of baking oven and is dried to constant weight, weighs duckweed dry weight, then duckweed is ground into powder, and Portugal is determined with HPLC
Grape sugared content, and content of starch is calculated, as a result as shown in Figure 1.
From figure 1 it appears that the content of starch of duckweed is as the extension of incubation time is into ascendant trend, in two days,
The content of starch of duckweed increases comparatively fast, and after culture 2 days, the content of starch of duckweed rises to 37.64%；After culture 7 days, float
The content of starch of duckweed is 66.80%, and after culture 10 days, the content of starch of duckweed is 70.49%, is the 10.8 of initial content
Times, in 7-10 days, content of starch increases slower.As a result show, in relative oligotrophic water body, uniconazole P and high concentration CO2
There is significantly facilitation to the content of starch of duckweed.
Embodiment two sprays uniconazole P and adds CO2It is (relatively rich that duckweed is cultivated in 1/5 concentration Hoagland nutrient solutions
Nutritional condition)
It is 54cm in surface area2Culture vessel in add 500mL1/5 concentration Hoagland nutrient solutions, take initial starch
Content is 6.52wt%, initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (covering
Rate is about 100%), immediately after to the duckweed surface uniconazole P solution that uniformly 1~2mL of sprinkling concentration is 500mg/L (with analysis
Pure uniconazole P is prepared), after sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is
25 DEG C, intensity of illumination be 130 μM of photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2It is dense
Degree is maintained near 650ppm.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day, the 5th
My god, the 7th day and the 10th day harvest duckweed sample, cleaned 3 times, then placed 5 minutes on filter paper, centrifugal dehydration 5 divides with distilled water
Clock, fresh duckweed is placed in 60 DEG C of baking oven after and is dried to constant weight, weigh duckweed dry weight, then duckweed is ground into powder, used
HPLC determines glucose content, and calculates content of starch, as a result as shown in Figure 2.
Figure it is seen that in sprinkling uniconazole P and it is passed through CO2Afterwards, with the extension of time, the content of starch of duckweed is fast
Speed increases, and content of starch reaches 20.01% two days later, is 3.1 times of initial content, and after culture 7 days, content of starch reaches
53.03%, after culture 10 days, content of starch reaches 61.03%, and starch reaches maximum, is 9.4 times of initial content, explanation
Spray uniconazole P and be passed through CO2The content of starch of duckweed can be increased substantially.
Embodiment three sprays uniconazole P and adds CO2Duckweed is cultivated in distilled water (with respect to oligotrophic condition)
It is 54cm in surface area2Culture vessel in add 500mL distilled water, take initial starch content for 6.52wt%,
Initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (coverage rate is about 100%), so
(matched somebody with somebody immediately to the duckweed surface uniconazole P solution that uniformly 1~2mL of sprinkling concentration is 800mg/L with analytically pure uniconazole P afterwards
System), after sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, illumination is strong
Spend for 130 μM of photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2Concentration be maintained at
Near 1200ppm.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day, the 5th day, the 7th
It harvested duckweed sample with the 10th day, is cleaned 3 times, is then placed 5 minutes on filter paper, centrifugal dehydration 5 minutes, after rear with distilled water
Fresh duckweed is placed in 60 DEG C of baking oven and is dried to constant weight, weighs duckweed dry weight, then duckweed is ground into powder, is determined with HPLC
Glucose content, and content of starch is calculated, as a result as shown in Figure 3.
From the figure 3, it may be seen that the content of starch of duckweed is in rising trend with incubation time, culture content of starch early stage increases fast
Speed, two days later content of starch can reach 52.79%, content of starch growth trend is gradually gentle after three days, culture 7 days after, duckweed
Content of starch is up to 75.16%, is 11.6 times of initial starch content, content of starch is not improved within 7~10 days.As a result
Show, in relative oligotrophic water body (N, P concentration are below 0.1ppm), uniconazole P and high concentration CO2To duckweed content of starch
There is significantly facilitation.
Example IV sprays uniconazole P and adds CO2It is (relatively rich that duckweed is cultivated in 1/5 concentration Hoagland nutrient solutions
Nutritional condition)
It is 54cm in surface area2Culture vessel in add 500mL1/5 concentration Hoagland nutrient solutions, take initial starch
Content is 6.52wt%, initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (covering
Rate is about 100%), immediately after to the duckweed surface uniconazole P solution that uniformly 1~2mL of sprinkling concentration is 800mg/L (with analysis
Pure uniconazole P is prepared), after sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is
25 DEG C, intensity of illumination be 130 μM of photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2It is dense
Degree is maintained near 1200ppm.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day,
5 days, the 7th day and the 10th day harvest duckweed samples, are cleaned 3 times with distilled water, are then placed 5 minutes on filter paper, centrifugal dehydration 5 divides
Clock, fresh duckweed is placed in 60 DEG C of baking oven after and is dried to constant weight, weigh duckweed dry weight, then duckweed is ground into powder, used
HPLC determines glucose content, and calculates content of starch, as a result as shown in Figure 4.
As shown in Figure 4, the content of starch of duckweed is in rising trend with incubation time, and content of starch is doubled and redoubled within first day,
Reach 27.40%, content of starch growth trend is gradually gentle afterwards, and after culture 7 days, duckweed content of starch reaches highest, from first
6.52% to begin brings up to 62.61%, and after cultivating 10 days, content of starch is slightly reduced to 59.84%.As a result show, relative
In eutrophic water body, uniconazole P and high concentration CO2The content of starch in duckweed can be significantly improved.
Embodiment five sprays uniconazole P and adds CO2Duckweed is cultivated in distilled water (with respect to oligotrophic condition)
It is 54cm in surface area2Culture vessel in add 500mL distilled water, take initial starch content for 6.52wt%,
Initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (coverage rate is about 80%), then
The uniconazole P solution (being prepared with analytically pure uniconazole P) that 1~2mL concentration is 100mg/L is uniformly sprayed to duckweed surface immediately,
After sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, intensity of illumination is
130μM photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2Concentration be maintained at 500ppm
Near.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day, the 5th day, the 7th day and the 10th
Its harvest duckweed sample, is cleaned 3 times with distilled water, is then placed 5 minutes on filter paper, centrifugal dehydration 5 minutes, by fresh duckweed after
It is placed in 60 DEG C of baking oven and is dried to constant weight, weigh duckweed dry weight, then duckweed is ground into powder, is contained with HPLC measure glucose
Amount, and content of starch is calculated, as a result display such as Fig. 5.
From fig. 5, it can be seen that in sprinkling uniconazole P and it is passed through CO2Afterwards, the content of starch of duckweed shows a rising trend, and is cultivating
After 3 days, content of starch reaches 40.28%, is 6 times of initial content, with the extension of incubation time, content of starch gradually increases
Add, but increase it is slower, culture 10 days after, the content of starch of duckweed reaches maximum, be 64.32%, illustrate uniconazole P with
CO2The growth of duckweed content of starch can be remarkably promoted.
Embodiment six sprays uniconazole P and adds CO2It is (relatively rich that duckweed is cultivated in 1/5 concentration Hoagland nutrient solutions
Nutritional condition)
It is 54cm in surface area2Culture vessel in add 500mL1/5 concentration Hoagland nutrient solutions, take initial starch
Content is 6.52wt%, initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (covering
Rate is about 80%), immediately after to the duckweed surface uniconazole P solution that uniformly 1~2mL of sprinkling concentration is 100mg/L (with analysis
Pure uniconazole P is prepared), after sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is
25 DEG C, intensity of illumination be 130 μM of photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2It is dense
Degree is maintained near 500ppm.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day, the 5th
My god, the 7th day and the 10th day harvest duckweed sample, cleaned 3 times, then placed 5 minutes on filter paper, centrifugal dehydration 5 divides with distilled water
Clock, fresh duckweed is placed in 60 DEG C of baking oven after and is dried to constant weight, weigh duckweed dry weight, then duckweed is ground into powder, used
HPLC determines glucose content, and calculates content of starch, as a result display such as Fig. 6.
From fig. 6, it can be seen that spray uniconazole P and be passed through CO2Afterwards, in 0-1 days of culture, content of starch growth is slower,
Since the 2nd day, content of starch increased sharply, and in the 7th day of culture, content of starch growth is relatively stable, after 7 days, duckweed
Content of starch be 36.81%, be 5.5 times of initial content, in culture 7-10 days, content of starch is not improved, and is being trained
After supporting 10 days, the content of starch of duckweed is 38.19%, illustrates uniconazole P and CO2The growth of duckweed content of starch can be remarkably promoted.
Embodiment seven sprays uniconazole P and adds CO2Duckweed is cultivated in distilled water (with respect to oligotrophic condition)
It is 54cm in surface area2Culture vessel in add 500mL distilled water, take initial starch content for 6.52wt%,
Initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (coverage rate is about 80%), then
The uniconazole P solution (being prepared with analytically pure uniconazole P) that 1~2mL concentration is 1000mg/L is uniformly sprayed to duckweed surface immediately,
After sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, intensity of illumination is
130μM photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2Concentration be maintained at 3000ppm
Near.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day, the 5th day, the 7th day and the 10th
Its harvest duckweed sample, is cleaned 3 times with distilled water, is then placed 5 minutes on filter paper, centrifugal dehydration 5 minutes, by fresh duckweed after
It is placed in 60 DEG C of baking oven and is dried to constant weight, weigh duckweed dry weight, then duckweed is ground into powder, is contained with HPLC measure glucose
Amount, and content of starch is calculated, as a result display such as Fig. 7.
From figure 7 it can be seen that in sprinkling uniconazole P and it is passed through CO2Afterwards, duckweed content of starch shows a rising trend, in culture 2
In it, content of starch increases comparatively fast, and content of starch reaches 32.74%, and with the extension of incubation time, content of starch gradually increases
Add, after culture 7 days, content of starch reaches 60.85%, and after cultivating 10 days, content of starch reaches 64.4%, is initial content
9.9 times, illustrate uniconazole P and the CO of high concentration2The growth of duckweed content of starch can be promoted.
Embodiment eight sprays uniconazole P and adds CO2It is (relatively rich that duckweed is cultivated in 1/5 concentration Hoagland nutrient solutions
Nutritional condition)
It is 54cm in surface area2Culture vessel in add 500mL1/5 concentration Hoagland nutrient solutions, take initial starch
Content is 6.52wt%, initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (covering
Rate is about 80%), immediately after to the duckweed surface uniconazole P solution that uniformly 1~2mL of sprinkling concentration is 1000mg/L (with analysis
Pure uniconazole P is prepared), after sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is
25 DEG C, intensity of illumination be 130 μM of photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2It is dense
Degree is maintained near 3000ppm.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day,
5 days, the 7th day and the 10th day harvest duckweed samples, are cleaned 3 times with distilled water, are then placed 5 minutes on filter paper, centrifugal dehydration 5 divides
Clock, fresh duckweed is placed in 60 DEG C of baking oven after and is dried to constant weight, weigh duckweed dry weight, then duckweed is ground into powder, used
HPLC determines glucose content, and calculates content of starch, as a result as shown in Figure 8.
From figure 8, it is seen that the content of starch of duckweed is in rising trend with the extension of incubation time, reached in incubation time
To after 10 days, the content of starch of duckweed reaches maximum, content of starch 54.79%, is 8.4 times of initial content, illustrates alkene
Imitate azoles and CO2Growth to duckweed content of starch has significant facilitation.
Embodiment nine sprays uniconazole P and adds CO2Duckweed is cultivated in distilled water (with respect to oligotrophic condition)
It is 54cm in surface area2Culture vessel in add 500mL distilled water, take initial starch content for 6.52wt%,
Initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (coverage rate is about 100%), so
Uniformly spray 1~2mL concentration 120mg/L uniconazole P solution (being prepared with analytically pure uniconazole P) to duckweed surface immediately afterwards,
After sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, intensity of illumination is
130μM photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2Concentration be maintained at 1300ppm
Near.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day, the 5th day, the 7th day and the 10th
Its harvest duckweed sample, is cleaned 3 times with distilled water, is then placed 5 minutes on filter paper, centrifugal dehydration 5 minutes, by fresh duckweed after
It is placed in 60 DEG C of baking oven and is dried to constant weight, weigh duckweed dry weight, then duckweed is ground into powder, is contained with HPLC measure glucose
Amount, and content of starch is calculated, as a result display such as Fig. 9.
It can be seen in figure 9 that with the extension of incubation time, the growth of duckweed content of starch is in rising trend, is training
After supporting 2 days, content of starch reaches 30.34%, is 4.6 times of initial content, and after cultivating 7 days, duckweed content of starch reaches maximum
Value, content of starch 69.77%, in culture 7-10 days, duckweed content of starch illustrated to spray uniconazole P and is passed through without rising appreciably
CO2The growth of duckweed content of starch can be remarkably promoted.
Embodiment ten sprays uniconazole P and adds CO2It is (relatively rich that duckweed is cultivated in 1/5 concentration Hoagland nutrient solutions
Nutritional condition)
It is 54cm in surface area2Culture vessel in add 500mL1/5 concentration Hoagland nutrient solutions, take initial starch
Content is 6.52wt%, initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (covering
Rate is about 100%), immediately after to the duckweed surface uniconazole P solution that uniformly 1~2mL of sprinkling concentration is 800mg/L (with analysis
Pure uniconazole P is prepared), after sprayed, CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is
25 DEG C, intensity of illumination be 130 μM of photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration makes CO2It is dense
Degree is maintained near 600ppm.Respectively CO is passed through in sprinkling uniconazole P solution2Afterwards the 0th day, the 1st day, the 2nd day, the 3rd day, the 5th
My god, the 7th day and the 10th day harvest duckweed sample, cleaned 3 times, then placed 5 minutes on filter paper, centrifugal dehydration 5 divides with distilled water
Clock, fresh duckweed is placed in 60 DEG C of baking oven after and is dried to constant weight, weigh duckweed dry weight, then duckweed is ground into powder, used
HPLC determines glucose content, and calculates content of starch, as a result display such as Figure 10.
From fig. 10 it can be seen that in sprinkling uniconazole P and it is passed through CO2Afterwards, the content of starch of duckweed increases in rising trend,
After culture 2 days, duckweed content of starch increases comparatively fast, and the duckweed content of starch after 2 days is 29.84%, after culture 10 days, floats
Duckweed content of starch reaches highest, peak 60.76%, is 9.3 times of initial duckweed content of starch, illustrate to spray uniconazole P and
It is passed through CO2The growth of duckweed content of starch can be promoted.
Comparative example one adds CO2Duckweed is cultivated in distilled water (with respect to oligotrophic condition)
It is 54cm in surface area2Culture vessel in add 500mL distilled water, take initial starch content for 6.52wt%,
Initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (coverage rate is about 100%), so
It is passed through CO at once afterwards2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, intensity of illumination is 130 μM
photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration, make CO2Concentration maintains 1200ppm or so.Point
CO is not being added2The 0th day, the 1st day, the 2nd day, the 3rd day, the 5th day, the 7th day and the 10th day harvest duckweed sample afterwards, with distillation
Water cleans 3 times, is then placed 5 minutes on filter paper, centrifugal dehydration 5 minutes, fresh duckweed is placed in 60 DEG C of baking oven after and dried
To constant weight, duckweed dry weight is weighed, then duckweed is ground into powder, glucose content is determined with HPLC, and calculates content of starch, such as
Shown in Figure 11.
It can be seen from figure 11 that under the conditions of relative oligotrophic, CO is passed through2Afterwards, duckweed content of starch increases sharply, and is training
After supporting 10 days, duckweed content of starch reaches peak, and duckweed content of starch is 72.21%.
Embodiments of the invention three and comparative example one are compared, under the conditions of relative oligotrophic, embodiment three sprays alkene
Imitate azoles and be passed through CO2, and comparative example one is only passed through CO2Although the content of starch peak of the two is not poor up and down, in embodiment three
Duckweed content of starch peak be 75.16%, the peak of the duckweed content of starch in comparative example one is 72.21%, still,
The incubation time that duckweed content of starch in embodiment three reaches peak is 7 days, and the duckweed content of starch in comparative example one
Reaching the incubation time of peak 10 days, the time when duckweed content of starch in embodiment three reaches highest shortens 30%,
Illustrate uniconazole P and the CO of high concentration2Content of starch accumulation to duckweed has obvious facilitation.
Comparative example two adds CO2Duckweed is cultivated in 1/5 concentration Hoagland nutrient solutions (with respect to eutrophy condition)
It is 54cm in surface area2Culture vessel in add 500mL 1/5 concentration Hoagland nutrient solutions, take initial shallow lake
Powder content is 6.52wt%, initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel and (cover
Lid rate is about 100%), to be then passed through CO at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, light
It is 130 μM of photons m according to intensity-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration, make CO2Concentration maintains
1200ppm or so.Adding CO2Duckweed sample is harvested after 10 days, is cleaned 3 times with distilled water, is then placed 5 minutes on filter paper,
Centrifugal dehydration 5 minutes, fresh duckweed is placed in 60 DEG C of baking oven after and is dried to constant weight, weigh duckweed dry weight, then by duckweed powder
Powder is broken into, glucose content is determined with HPLC, and calculates content of starch, as a result as shown in figure 12.
It can be recognized from fig. 12 that it is passed through CO under the conditions of relative eutrophy2Afterwards, the content of starch of duckweed is in rising trend,
After culture 7 days, the content of starch of duckweed reaches highest, peak 33.21%.
Example IV and comparative example two are compared, under the conditions of relative eutrophy, example IV is floated after culture 7 days
The content of starch of duckweed reaches highest, and peak 62.61%, after culture 7 days, the content of starch of duckweed reaches most comparative example two
Height, up to 33.21%, illustrate to spray uniconazole P and be passed through CO2The content of starch of duckweed can be promoted to increase, example IV exists
After culture 2 days, the content of starch in duckweed is 32.5%, and the content of starch of duckweed after cultivating 7 days of comparative example two just reaches
33.21%, illustrate uniconazole P and CO2Accumulation to the content of starch of duckweed has significant facilitation.
Comparative example three adds CO2Duckweed is cultivated in distilled water (with respect to oligotrophic condition)
It is 54cm in surface area2Culture vessel in add 500mL distilled water, take initial starch content for 6.52wt%,
Initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (coverage rate is about 80%), then
CO is passed through at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, intensity of illumination is 130 μM
photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration, make CO2Concentration maintains 500ppm or so.Point
CO is not being added2The 0th day, the 1st day, the 2nd day, the 3rd day, the 5th day, the 7th day and the 10th day harvest duckweed sample afterwards, with distillation
Water cleans 3 times, is then placed 5 minutes on filter paper, centrifugal dehydration 5 minutes, fresh duckweed is placed in 60 DEG C of baking oven after and dried
To constant weight, duckweed dry weight is weighed, then duckweed is ground into powder, glucose content is determined with HPLC, and calculates content of starch, is tied
Fruit is as shown at 13.
As can be seen from Figure 13, under the conditions of relative oligotrophic, CO is added2Afterwards, the content of starch of duckweed is in rising trend,
After culture 1 day, the increase of duckweed content of starch is very fast, and after culture the 10th, duckweed content of starch reaches highest, and peak is
62.43%.
Embodiment five and comparative example three are compared, under the conditions of relative oligotrophic, embodiment five is floated after culture 10 days
Duckweed content of starch reaches peak, and peak 64.32%, after incubation time 10 days, duckweed content of starch reaches comparative example three
To peak, peak 62.43%, although embodiment five, compared with comparative example three, the growth of duckweed content of starch is not notable,
It is to spray uniconazole P and be passed through CO2The content of starch of duckweed can be promoted to increase.
Comparative example four adds CO2Duckweed is cultivated in 1/5 concentration Hoagland nutrient solutions (with respect to eutrophy condition)
It is 54cm in surface area2Culture vessel in add 500mL 1/5 concentration Hoagland nutrient solutions, take initial shallow lake
Powder content is 6.52wt%, initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel and (cover
Lid rate is about 80%), to be then passed through CO at once2, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, illumination
Intensity is 130 μM of photons m-2s-1, the CO in No. 2 incubators in air is determined daily2Concentration, make CO2Concentration maintains
3000ppm or so.Adding CO2Duckweed sample is harvested after 10 days, is cleaned 3 times with distilled water, is then placed 5 minutes on filter paper,
Centrifugal dehydration 5 minutes, fresh duckweed is placed in 60 DEG C of baking oven after and is dried to constant weight, weigh duckweed dry weight, then by duckweed powder
Powder is broken into, glucose content is determined with HPLC, and calculates content of starch, as a result as shown in figure 14.
It is seen from figure 14 that under the conditions of relative eutrophy, CO is passed through2Afterwards, the content of starch of duckweed is in rising trend,
After culture 10 days, the content of starch of duckweed reaches highest, peak 31.34wt%.
Embodiment eight and comparative example four are compared, under the conditions of relative eutrophy, embodiment eight sprinkling uniconazole P and
It is passed through CO2Afterwards, the content of starch of duckweed reaches highest, peak 54.79% after cultivating 10 days, and comparative example four is being passed through
CO2Afterwards, duckweed content of starch reaches highest after cultivating 10 days, peak 31.34%, illustrates uniconazole P and CO2Duckweed is formed sediment
The growth of powder content has significant facilitation.
Comparative example five sprays uniconazole P and duckweed is cultivated in distilled water (with respect to oligotrophic condition)
It is 54cm in surface area2Culture vessel in add 500mL distilled water, take initial starch content for 6.52wt%,
Initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel (coverage rate is about 100%), so
(matched somebody with somebody immediately to the duckweed surface uniconazole P solution that uniformly 1~2mL of sprinkling concentration is 800mg/L with analytically pure uniconazole P afterwards
System), cultivated 10 days under the conditions of full exposure after sprayed, wherein, cultivation temperature is 25 DEG C, intensity of illumination is 130 μM
photons m-2s-1.The 0th day after uniconazole P solution is sprayed, the 1st day, the 2nd day, the 3rd day, the 5th day, the 7th day and respectively
10 days harvest duckweed samples, are cleaned 3 times with distilled water, are then placed 5 minutes on filter paper, centrifugal dehydration 5 minutes, will be fresh floating after
Duckweed is placed in 60 DEG C of baking oven and is dried to constant weight, weighs duckweed dry weight, then duckweed is ground into powder, and glucose is determined with HPLC
Content, and content of starch is calculated, as a result as shown in figure 15.
It can be seen from fig. 15 that under the conditions of relative oligotrophic, after uniconazole P is sprayed, after culture 1 day and 2 days, float
The content of starch of duckweed increases comparatively fast, and after culture 3 days, duckweed content of starch declines, after culture 5-10 days, duckweed content of starch
In rising trend, after culture 10 days, content of starch reaches highest, peak 72.06wt%.
Embodiment three and comparative example five are compared, under the conditions of relative oligotrophic, embodiment three sprinkling uniconazole P and
It is passed through CO2Afterwards, after culture 7 days, the content of starch of duckweed reaches highest, peak 75.16%, and comparative example five is being sprayed
After uniconazole P, after culture 10 days, the content of starch of duckweed reaches highest, peak 72.16%, when reaching highest level
Time shorten 30%, illustrate uniconazole P and CO2Accumulation to duckweed content of starch has significant facilitation.
Comparative example six sprays uniconazole P and duckweed is cultivated in 1/5 concentration Hoagland nutrient solutions (with respect to eutrophy condition)
It is 54cm in surface area2Culture vessel in add 500mL 1/5 concentration Hoagland nutrient solutions, take initial shallow lake
Powder content is 6.52wt%, and initial biomass is calculated as 21g/m with dry weight2Fresh duckweed 1.0g be transferred in the culture vessel and (cover
Lid rate is about 100%), immediately after to the duckweed surface uniconazole P solution that uniformly 1~2mL of sprinkling concentration is 800mg/L (to divide
Pure uniconazole P is analysed to prepare), after sprayed, cultivated 10 days under the conditions of full exposure, wherein, cultivation temperature is 25 DEG C, illumination
Intensity is 130 μM of photons m-2s-1.After incubation time expires, duckweed sample is taken, 3 times are cleaned with distilled water, then with washing
The dehydration of clothing machine is dried, and fresh duckweed is placed in 60 DEG C of baking oven after and is dried to constant weight, weighs duckweed dry weight, then duckweed is crushed
Into powder, glucose content is determined with HPLC, and calculates content of starch, as a result as shown in figure 16.
As can be seen from Figure 16, under the conditions of relative eutrophy, after spraying uniconazole P, after culture 2 days, content of starch shows
Increase is write, in culture 2 days, content of starch increased to 21.20%, and with the extension of incubation time, content of starch gradually increases,
After culture 10 days, content of starch reaches 46.92%.
Example IV and comparative example six are compared, example IV sprays uniconazole P and add CO under the conditions of eutrophy2
Afterwards, after culture 1 day, content of starch is doubled and redoubled, and content of starch reaches 27.40% or so, after culture 7 days, content of starch
Reach highest, content of starch 62.61%, and comparative example six is after uniconazole P is sprayed, after culture 1 day, content of starch is
12.38%, after culture 10 days, content of starch 46.92%, the content of starch highest time of example IV is in culture 7
After it, and the incubation time of comparative example six is after 10 days, and the content of starch of example IV is up to 62.61%, and comparative example six
Content of starch be up to 46.92%, it can be seen that, uniconazole P and CO2The content of starch of duckweed can be improved, and to duckweed
Content of starch accumulation has obvious facilitation.
Embodiment ten and comparative example six are compared, although being all the extension with incubation time, content of starch gradually increases
Add, but content of starch of the embodiment ten after cultivating 10 days is 60.76%, and content of starch of the comparative example six after cultivating 10 days
For 46.92%, illustrate uniconazole P and CO2The content of starch of duckweed can be significantly improved.
In summary, the present invention uses uniconazole P and CO2, the growth of duckweed content of starch can be promoted, and duckweed is formed sediment
The accumulation of powder content has significant facilitation, and a duckweed rapid growth in different water bodys, is especially being contaminated
The duckweed of large-scale culture high-content of starch in water body, nitrogen phosphorus in sewage is also achieved while Rapid Accumulation high-content of starch
Efficient removal.
It is described above, only it is the preferred embodiment that the present invention is implemented, any formal limitation not is done to the present invention, it is all
Modifications, equivalent substitutions and improvements done within the spirit and principles in the present invention etc., it is required to the protection included in the present invention
Within the scope of.
Claims (10)
1. a kind of cultural method for improving duckweed content of starch, it is characterised in that it includes following step：Duckweed is inoculated in water
Body surface face, uniconazole P solution then is sprayed to the duckweed surface being grown in water body, is then passed through CO2, obtain target product and float
Duckweed.
2. cultural method according to claim 1, it is characterised in that the CO2Concentration be 500-3000ppm, be preferably
650-1200ppm。
3. cultural method according to claim 1 or 2, it is characterised in that the concentration of the uniconazole P be 100~
1000mg/L, preferably 500~800mg/L.
4. according to the cultural method described in claim any one of 1-3, it is characterised in that the fountain height of the uniconazole P solution is
Every square metre of 150~300mL of duckweed.
5. according to the cultural method described in claim any one of 1-4, it is characterised in that be passed through CO2After cultivated, cultivate
Time >=7 day, preferably 7-10 days.
6. according to the cultural method described in claim any one of 1-5, it is characterised in that the culture is to be trained under light illumination
Support, the illumination condition is full exposure.
7. according to the cultural method described in claim any one of 1-6, it is characterised in that the water body is relative eutrophic water body
Or relative oligotrophic water body.
8. according to the cultural method described in claim any one of 1-7, it is characterised in that will according to 80%-100% coverage rate
Duckweed is inoculated in water surface.
9. according to the cultural method described in claim any one of 1-8, it is to cultivate duckweed in the water body of pollution.
10. cultural method according to claim 9, its application in purifying contaminated water body.
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| CN108782204A (en) * | 2018-06-26 | 2018-11-13 | 中国科学院成都生物研究所 | A method of improving duckweed total starch yield using methylation inhibitor |
| CN114041409A (en) * | 2021-08-20 | 2022-02-15 | 天津师范大学 | Culture method of high-protein duckweed |
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| CN103609304A (en) * | 2013-11-22 | 2014-03-05 | 中国科学院成都生物研究所 | Method for quickly increasing content of starch in duckweed |
| CN103947529A (en) * | 2014-05-12 | 2014-07-30 | 中国科学院成都生物研究所 | Method for simultaneously improving yield of starch and crude protein of duckweed and removal rate of nitrogen and phosphorus in sewage |
| CN105454026A (en) * | 2015-11-19 | 2016-04-06 | 中国科学院青岛生物能源与过程研究所 | Duckweed stereoscopic culturing apparatus |
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| JP2007222123A (en) * | 2006-02-27 | 2007-09-06 | National Agriculture & Food Research Organization | Quality improvement and nitric acid-reducing technique for edible plant by supply of peptides |
| CN103609304A (en) * | 2013-11-22 | 2014-03-05 | 中国科学院成都生物研究所 | Method for quickly increasing content of starch in duckweed |
| CN103947529A (en) * | 2014-05-12 | 2014-07-30 | 中国科学院成都生物研究所 | Method for simultaneously improving yield of starch and crude protein of duckweed and removal rate of nitrogen and phosphorus in sewage |
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| CN114041409A (en) * | 2021-08-20 | 2022-02-15 | 天津师范大学 | Culture method of high-protein duckweed |
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