CN101705188B - Method for domestically culturing salt-water Spirulina - Google Patents
Method for domestically culturing salt-water Spirulina Download PDFInfo
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- CN101705188B CN101705188B CN200910114593A CN200910114593A CN101705188B CN 101705188 B CN101705188 B CN 101705188B CN 200910114593 A CN200910114593 A CN 200910114593A CN 200910114593 A CN200910114593 A CN 200910114593A CN 101705188 B CN101705188 B CN 101705188B
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000012258 culturing Methods 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 12
- 235000016425 Arthrospira platensis Nutrition 0.000 title abstract description 17
- 240000002900 Arthrospira platensis Species 0.000 title abstract description 17
- 229940082787 spirulina Drugs 0.000 title abstract description 17
- 230000012010 growth Effects 0.000 claims abstract description 8
- 238000006213 oxygenation reaction Methods 0.000 claims abstract description 6
- 241001517315 Habenaria Species 0.000 claims description 59
- 238000003756 stirring Methods 0.000 claims description 14
- 241000195493 Cryptophyta Species 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 11
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 10
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 206010036590 Premature baby Diseases 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- LXAHHHIGZXPRKQ-UHFFFAOYSA-N 5-fluoro-2-methylpyridine Chemical compound CC1=CC=C(F)C=N1 LXAHHHIGZXPRKQ-UHFFFAOYSA-N 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- 208000006558 Dental Calculus Diseases 0.000 claims description 5
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 5
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 5
- 239000004327 boric acid Substances 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 238000011161 development Methods 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 5
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 5
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 5
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 claims description 5
- DCKVFVYPWDKYDN-UHFFFAOYSA-L oxygen(2-);titanium(4+);sulfate Chemical compound [O-2].[Ti+4].[O-]S([O-])(=O)=O DCKVFVYPWDKYDN-UHFFFAOYSA-L 0.000 claims description 5
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 5
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 5
- 239000004317 sodium nitrate Substances 0.000 claims description 5
- 229940001516 sodium nitrate Drugs 0.000 claims description 5
- 235000010344 sodium nitrate Nutrition 0.000 claims description 5
- 229910000348 titanium sulfate Inorganic materials 0.000 claims description 5
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 5
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 5
- 229960001763 zinc sulfate Drugs 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 4
- 241000251468 Actinopterygii Species 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 210000002421 cell wall Anatomy 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract description 2
- 238000012136 culture method Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 238000005286 illumination Methods 0.000 abstract 1
- 235000015816 nutrient absorption Nutrition 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 description 4
- 239000013505 freshwater Substances 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 241000192700 Cyanobacteria Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
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- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
Abstract
The invention discloses a method for domestically culturing Spirulina. The traditional Spirulina culture needs a culture pond suitable for Spirulina growth, while the domestic culture of the Spirulina still belongs to the field of unnoticeable technical progress at present. The domestic culture of the Spirulina is like domestic culture of fish and flowers, and not only can beautify domestic environment but also can obtain quality-guaranteed fresh Spirulina for eating. The method comprises the following steps: firstly preparing culture solution, putting the culture solution to a domestic Spirulina culture container, and then putting Spirulina strains; and culturing for 2 to 8 days, controlling water temperature, illumination and oxygenation in the period, culturing until the Spirulina is ripe, and harvesting and eating the Spirulina. Through detection, the Spirulina cultured by the method has the advantages of large number of spirals, long filaments, large cells and loose cell walls, and has the characteristics of easy absorption of human bodies, and over 95 percent of nutrient absorption rate; and the culture method is simple and convenient, and the cleaning is easy.
Description
Technical field
What the present invention relates to is a kind of method of culturing tenaculat Habenaria, relate in particular to a kind of can be popular, the method for the family-raise tenaculat Habenaria of generalization and simplification.
Background technology
Tenaculat Habenaria ownership Cyanophyta, Cyanophyceae, disconnected body order, Oscillariaceae, the Spirullina of growing; Being the phycophyta that a kind of cell physiological result is similar to bacterium, being blackish green, is that a kind of photosynthetic autotrophs are biological; Be the abundantest, the isostatic natural nutrition subsidy food of the nutrition found up to now; It contains human daily necessary protein, and constitutes proteinic amino acid composition and be in admirable proportion, and also some human body is difficult to the composition that obtains from other food; And its digestion is up to 95%, very easily by human consumption and absorption.The tenaculat Habenaria product has been applied to the human consumption field at present, and tenaculat Habenaria has anti-virus ability preferably, and effectively lowering blood glucose, SUV are a kind of higher medical health care products that is worth.Traditional tenaculat Habenaria all adopts freshwater aquiculture or sea farming, is called fresh water tenaculat Habenaria or seawater spirulina, these two kinds of breed modes; The open-air formula of basic employing is cultured; Utilize natural lake or build large-scale breed pond and culture, the advantage of this breeding way is that cost is low, and shortcoming is that quality product is difficult to guarantee; Unit output is lower, receives the influence in environment and season easily.Such industrialized culture; Culture the tenaculat Habenaria that comes out and just can become the edible food of people through industrialized processing again; In the breed and the course of processing, can cause loss of nutrients unavoidably or bring impurity into, thereby reduce the quality and the trophic function of tenaculat Habenaria.And the family-raise tenaculat Habenaria still belongs to the field that technical progress owes to pay close attention at present; The family-raise tenaculat Habenaria just looks like to breed fish in the family, grow flower equally, can beautify home environment on the one hand; The fresh spirulina that can obtain quality-guarantee on the other hand is edible, to replenish protein.Through retrieval, find that publication number is CN1254012, title is the invention application of " a kind of method of domestic edible fresh Spirulina "; Disclose a kind of method of domestic tenaculat Habenaria, though this method has the characteristics of himself, used nutrient solution prescription is complicated; Raw materials used have a kind more than 20, and cultural method is cumbersome, and step is too many; Simple with cultural method, the requirement of implementing easily greatly differs from each other.Therefore, a kind of method of effective simple and practical family-raise tenaculat Habenaria being provided is highly significant.
Summary of the invention
The method that the purpose of this invention is to provide a kind of family-raise tenaculat Habenaria; Be subject to the influence in environment and season, bring the deficiency of impurity into easily to overcome the industrialized culture tenaculat Habenaria, thus the spirulina breeding method is become be not subject to ectocine, controlled, having can successional cultural method.
In order to reach above purpose, the present invention adopts following technical scheme, and a kind of method of family-raise tenaculat Habenaria may further comprise the steps:
(1) it is subsequent use to get sodium hydrogencarbonate 8.0-13.9 gram, potassium hydrogenphosphate 0.1-0.5 gram, SODIUMNITRATE 2.1-2.8 gram, vitriolate of tartar 0.8-1.2 gram, sal epsom 0.1-0.5 gram, calcium chloride 0.01-0.05 gram, ferric sulfate 0.01-0.05 gram, EDTA Disodium 0.05-0.08 gram, sodium-chlor 3.6-4.2 gram, boric acid 0.01-0.09 gram, Manganous chloride tetrahydrate 0.01-0.05 gram, zinc sulfate 0.01-0.05 gram, titanium sulfate 0.01-0.03 gram and Xiao Suangu 0.01-0.05 gram;
(2) sodium hydrogencarbonate in the aforementioned proportion prescription, potassium hydrogenphosphate, SODIUMNITRATE, vitriolate of tartar, sal epsom, calcium chloride, ferric sulfate, EDTA Disodium and sodium-chlor are added mixing in the entry in order; And in adition process, do not stop to stir, till dissolving; Again boric acid, Manganous chloride tetrahydrate, zinc sulfate, titanium sulfate, Xiao Suangu are sequentially added into mixing in the water, and in adition process, do not stop to stir, till dissolving; Above-mentioned two kinds of solution are mixed and stir, and water is adjusted to 1 liter, leave standstill, getting supernatant and transferring pH value is 8.5-10, and it is subsequent use to obtain nutrient solution;
The amount of (3) in above-mentioned nutrient solution, pressing the 3-8 grams per liter adds tenaculat Habenaria algae kind, stirs, and the algae kind was uniformly distributed in the culturing liquid development growth 2-8 days, during water temperature remain on 18 ℃-38 ℃, oxygenation 24 hours every days also guarantees that every day, light application time reached 6-18 hour;
(4) tenaculat Habenaria reaches ripe after above-mentioned breed; The string bag with 200-300 order filtering net is gathered; Ripe tenaculat Habenaria is stayed on the filtering net, and still immature tenaculat Habenaria then flows into again in company with culturing liquid through filtering net and repeats said process in the Cultivation container again, so far ripe tenaculat Habenaria is separated with the prematurity tenaculat Habenaria; Ripe tenaculat Habenaria is edible, and the prematurity tenaculat Habenaria is uninterrupted continuously cultures.
Taking the present invention of above-mentioned measure, is a kind of method of suitable family-raise tenaculat Habenaria, and the tenaculat Habenaria that breed is come out is through detecting; It is many to have the spiral number, and filament is longer, and cell is big; The advantage that cell walls is more loose, so be absorbed by the body easily, the dietetic alimentation rate is up to the characteristics more than 90%.And cultural method is easy, and cleaning easily.Culture tenaculat Habenaria with the present invention, breeding environment can be located at indoor, therefore can reduce the pollution of environment; Carry out aseptic technique, the content of its trace element components is abundant and balanced than the fresh water tenaculat Habenaria, contains than the more active factor of fresh water tenaculat Habenaria; Tenaculat Habenaria with the present invention's breed; Through detecting, contain active substance polysaccharides, inositol and Phycocyanins, C-, and the content of protein, Y-linolenic acid and Phycocyanins, C-is all higher.Culture tenaculat Habenaria with the present invention, can also keep the pure of tenaculat Habenaria algae kind.Culture tenaculat Habenaria with the present invention, can promote spirulina growth, shorten the culture-cycle of tenaculat Habenaria, through experiment, get 250 ml flasks and inoculate 200 milliliters of algae liquid in proportion, every day, timing sampling was counted cell count at microscopically, obtained following experimental data:
| Fate | The tenaculat Habenaria that the present invention cultures | The tenaculat Habenaria that common cultural method is cultured |
| 0 | 3.33E+06 | 3.40E+06 |
| 1 | 7.49E+06 | 8.30E+06 |
| 2 | 1.88E+07 | 1.42E+07 |
| 3 | 3.06E+07 | 1.58E+07 |
| 4 | 4.90E+07 | 4.22E+07 |
| 5 | 5.18E+07 | 5.15E+07 |
| 6 | 6.66E+07 | 4.98E+07 |
Can know by above-mentioned experimental data, with the tenaculat Habenaria that the present invention cultures, the peak period that got into vegetative period since the 2nd day, and the tenaculat Habenaria of culturing with common cultural method gets into the summit of growth phase since the 4th talent.Prompting thus, the tenaculat Habenaria with the present invention cultures has obviously shortened growth cycle, has significantly reduced aquaculture cost.
Embodiment
Embodiment 1
1, get prescription:
2, sodium hydrogencarbonate, potassium hydrogenphosphate, SODIUMNITRATE, vitriolate of tartar, sal epsom, calcium chloride, ferric sulfate, EDTA Disodium and the sodium-chlor got in the above-mentioned prescription add mixing in 80 premium on currency in order; In adition process, do not stop to stir, till dissolving; Boric acid, Manganous chloride tetrahydrate, zinc sulfate, titanium sulfate, the Xiao Suangu got in the above-mentioned prescription are sequentially added into mixing in 15 premium on currency again, and in adition process, do not stop to stir, till dissolving; Above-mentioned two kinds of solution mixed and stir, and water is adjusted to 100 liters, leave standstill, supernatant and to transfer pH value to obtain nutrient solution to 8-10 subsequent use;
3, in above-mentioned nutrient solution, add 300 gram tenaculat Habenaria algae kinds, stir, make the algae kind be uniformly distributed in the culturing liquid development growth 7 days, during water temperature remain on 20-25 ℃, oxygenation 24 hours every days also guarantees that every day, light application time reached 6 hours;
4, tenaculat Habenaria reaches ripe after above-mentioned breed; The string bag with 300 order filtering nets is gathered; Ripe tenaculat Habenaria is stayed on the filtering net, and still immature tenaculat Habenaria then flows into again in company with culturing liquid through filtering net and repeats said process in the Cultivation container again, so far ripe tenaculat Habenaria is separated with the prematurity tenaculat Habenaria; Ripe tenaculat Habenaria is edible, and the prematurity tenaculat Habenaria can uninterruptedly be cultured under the prerequisite of in good time replenishing nutrient solution continuously.
Embodiment 2
1, get prescription:
2, the step 2 with embodiment 1 is identical;
3, in above-mentioned nutrient solution, add 800 gram tenaculat Habenaria algae kinds, stir, make the algae kind be uniformly distributed in the culturing liquid development growth 5 days, during water temperature remain on 20-25 ℃, oxygenation 24 hours every days also guarantees that every day, light application time reached 12 hours;
4, the step 4 with embodiment 1 is identical.
Embodiment 3
1, get prescription:
2, the step 2 with embodiment 1 is identical;
3, in above-mentioned nutrient solution, add 500 gram tenaculat Habenaria algae kinds, stir, make the algae kind be uniformly distributed in the culturing liquid development growth 7 days, during water temperature remain on 20-25 ℃, oxygenation 24 hours every days also guarantees that every day, light application time reached 18 hours;
4, the step 4 with embodiment 1 is identical.
Claims (1)
1. the method for a family-raise tenaculat Habenaria may further comprise the steps:
(1) it is subsequent use to get sodium hydrogencarbonate 8.0-13.9 gram, potassium hydrogenphosphate 0.1-0.5 gram, SODIUMNITRATE 2.1-2.8 gram, vitriolate of tartar 0.8-1.2 gram, sal epsom 0.1-0.5 gram, calcium chloride 0.01-0.05 gram, ferric sulfate 0.01-0.05 gram, EDTA Disodium 0.05-0.08 gram, sodium-chlor 3.6-4.2 gram, boric acid 0.01-0.09 gram, Manganous chloride tetrahydrate 0.01-0.05 gram, zinc sulfate 0.01-0.05 gram, titanium sulfate 0.01-0.03 gram and Xiao Suangu 0.01-0.05 gram;
(2) sodium hydrogencarbonate in the above-mentioned prescription, potassium hydrogenphosphate, SODIUMNITRATE, vitriolate of tartar, sal epsom, calcium chloride, ferric sulfate, EDTA Disodium and sodium-chlor are added mixing in the entry in order; And in adition process, do not stop to stir, till dissolving; Again boric acid, Manganous chloride tetrahydrate, zinc sulfate, titanium sulfate, Xiao Suangu are sequentially added into mixing in the water, and in adition process, do not stop to stir, till dissolving; Above-mentioned two kinds of solution are mixed and stir, and water is adjusted to 1 liter, leave standstill, getting supernatant and transferring pH value is 8.5-10, and it is subsequent use to obtain nutrient solution;
The amount of (3) in above-mentioned nutrient solution, pressing the 3-8 grams per liter adds tenaculat Habenaria algae kind, stirs, and the algae kind was uniformly distributed in the culturing liquid development growth 2-8 days, during water temperature remain on 18 ℃-38 ℃, oxygenation 24 hours every days also guarantees that every day, light application time reached 6-18 hour;
(4) tenaculat Habenaria reaches ripe after above-mentioned breed; The string bag with 200-300 order filtering net is gathered; Ripe tenaculat Habenaria is stayed on the filtering net, and still immature tenaculat Habenaria then flows into again in company with culturing liquid through filtering net and repeats said process in the Cultivation container again, so far ripe tenaculat Habenaria is separated with the prematurity tenaculat Habenaria; Ripe tenaculat Habenaria is edible, and the prematurity tenaculat Habenaria is uninterrupted continuously cultures.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910114593A CN101705188B (en) | 2009-11-30 | 2009-11-30 | Method for domestically culturing salt-water Spirulina |
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| CN200910114593A CN101705188B (en) | 2009-11-30 | 2009-11-30 | Method for domestically culturing salt-water Spirulina |
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| CN101705188B true CN101705188B (en) | 2012-09-05 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586491B (en) * | 2012-03-02 | 2013-08-07 | 云南绿A生物工程有限公司 | Method for controlling heavy metals in process of culturing spirulina |
| CN104017762A (en) * | 2014-06-25 | 2014-09-03 | 京环央都南通生物医药研究院有限公司 | Culture method of spirulina |
| CN109234209A (en) * | 2018-11-16 | 2019-01-18 | 杭州源生泰生物科技股份有限公司 | A kind of method of domestic edible spirulina |
| KR102064189B1 (en) * | 2019-08-30 | 2020-01-09 | 주식회사 엔셀 | Novel Spirulina platensis strain |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RO111470B1 (en) * | 1995-05-15 | 1996-10-31 | Centrul De Cercetari Pentru Te | Form of spirulina platensis ( nordst ) geitl which is growing environment and cultivating process in continuous flow for spiruline platensis mutant (nordst) geitl |
| CN1510123A (en) * | 2002-12-23 | 2004-07-07 | 尹伟伦 | Culture of spirulina by concentrated Na2 Co3-NaHCO3 saline-alakli lake water |
-
2009
- 2009-11-30 CN CN200910114593A patent/CN101705188B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RO111470B1 (en) * | 1995-05-15 | 1996-10-31 | Centrul De Cercetari Pentru Te | Form of spirulina platensis ( nordst ) geitl which is growing environment and cultivating process in continuous flow for spiruline platensis mutant (nordst) geitl |
| CN1510123A (en) * | 2002-12-23 | 2004-07-07 | 尹伟伦 | Culture of spirulina by concentrated Na2 Co3-NaHCO3 saline-alakli lake water |
Non-Patent Citations (2)
| Title |
|---|
| OLIVEIRA,M.A.C.L.DE et al.Growth and chemical composition of spirulina maxima and spirulina platensis biomass at different temperatures.《AQUACULTURE INTERNATIONAL》.1999,第7卷(第4期),261-275. * |
| 薛志成.家庭养殖螺旋藻技术.《科学养殖》.1985,(第5期),22. * |
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