CN1510123A - Culture of spirulina by concentrated Na2 Co3-NaHCO3 saline-alakli lake water - Google Patents
Culture of spirulina by concentrated Na2 Co3-NaHCO3 saline-alakli lake water Download PDFInfo
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- CN1510123A CN1510123A CNA021573727A CN02157372A CN1510123A CN 1510123 A CN1510123 A CN 1510123A CN A021573727 A CNA021573727 A CN A021573727A CN 02157372 A CN02157372 A CN 02157372A CN 1510123 A CN1510123 A CN 1510123A
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- China
- Prior art keywords
- spirulina
- concentrated solution
- lake water
- medium
- saline
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Links
- 235000016425 Arthrospira platensis Nutrition 0.000 title claims abstract description 37
- 240000002900 Arthrospira platensis Species 0.000 title claims abstract description 37
- 229940082787 spirulina Drugs 0.000 title claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 17
- WCTAGTRAWPDFQO-UHFFFAOYSA-K trisodium;hydrogen carbonate;carbonate Chemical compound [Na+].[Na+].[Na+].OC([O-])=O.[O-]C([O-])=O WCTAGTRAWPDFQO-UHFFFAOYSA-K 0.000 title abstract 2
- 238000000034 method Methods 0.000 claims abstract description 10
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000004202 carbamide Substances 0.000 claims abstract description 3
- 235000015097 nutrients Nutrition 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- 238000009395 breeding Methods 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 2
- -1 phosphoric acid diamines Chemical class 0.000 claims description 2
- 239000011573 trace mineral Substances 0.000 claims description 2
- 235000013619 trace mineral Nutrition 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 abstract 2
- 239000005696 Diammonium phosphate Substances 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 abstract 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 abstract 1
- 235000019838 diammonium phosphate Nutrition 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 235000010344 sodium nitrate Nutrition 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 17
- 235000013305 food Nutrition 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000192700 Cyanobacteria Species 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000002349 well water Substances 0.000 description 1
- 235000020681 well water Nutrition 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A method for culturing spirulina in large scale by using the concentrated Na2CO3-NaHCO3 type salt lake water features that its culture liquid is prepared from said concentrated salt lake water, nitrogen source (NaNO3 or urea), diammonium phosphate and Na-EDTA. Its pH value is 8.5-11. Its advantages are high output of spirulina (up to 6-8 g/sq.m.d), and low cost decreased by 85-95%.
Description
The present invention relates to utilize Na
2CO
3-NaHCO
3The method of the saline and alkaline lake water concentrated solution of type scale operation spirulina, spirulina is a kind of algae of the alkaline waters of growing, its classification position belongs to Cyanophyta, Cyanophyceae, hormogon[ium order, a genus--the Spirullina in the Oscillariaceae.Spirulina plalensis and spirulina maxim are two kinds that Spirullina can industrialization.
Spirulina is rich in nutrition, and wherein protein content is up to 60～70%, and protein and amino acid are formed the food sanitation standard that meets United Nations's regulation.In addition, VITAMIN, carotene, polysaccharide, mineral substance equal size enrich, be recommended as human optimal food by United Nations, therefore, domestic and international many enterprises are devoted to exploitation and produce spirulina, the prescription that used nutrient solution prescription is the Zarrouk nutrient solution or adjusts a little, artificial preparation nutrient solution, producer need purchase nearly kind of chemical feedstocks surplus in the of 15～20, and the height of aquaculture cost makes product be difficult to enter general marketplace.Nearly 20 years of domestic scale production; very remote apart from popularizing of spirulina product; still be applied in the medicines and health protection field; compare as these characteristics of the optimal food of the mankind with spirulina; its development pole is inharmonious; major reason just has been to use expensive nutrient solution; yet; the inventor has found new Chinese record species--spirulina plalensis first at Inner Mongol Ordos Plateau salt alkali lake; through in a few years studying and testing; invent a whole set of and utilized saline and alkaline lake water concentrated solution; be fit to the method for the large-scale cultivation spirulina (spirulina plalensis and the spirulina maxim that are fit to import equally) of local condition, performing step is as follows:
1. with Na
2CO
3-NaHCO
3The saline and alkaline lake water of type is through natural evaporation concentration, and obtaining degree Beaume is 10～28 degree concentrated solutions.The biological net filtration injection of these solution 400～600 orders cultivation pool makes at the bottom of the pond and pool wall evenly soaks concentrated solution, keeps 10～24 hours.Reach the purpose of killing other biological.To concentrate lake water and adjust to 1～6 degree Beaume, add nitrogenous source (as NaNO
31～2.5g/L, urea 00.3～0.1g/L), phosphoric acid diamines 0.3～0.7g/L, Na-EDTA0.03～0.1g/L is mixed with the nutrient solution that is suitable for growth of spirulina platensis, in breeding process, the pH value 8.5～11 of control nutrient solution.Spirulina output just can reach 6～8g/m
2D, this output has reached the mean level (ML) that domestic racetrack is cultured.Compare with artificial preparation Zarrouk nutrient solution, it is about 5～8% that aforesaid method prepared culture chemistry solute only need add, and saves about 92～95% chemical solute and need not add, and makes aquaculture cost descend 85～95%.
2. above-mentioned 1. spirulina mediums that are made into further improve spirulina output through adding trace element to its fine setting, can reach 8～12g/m
2D, this has reached the advanced level that international racetrack is cultured spirulina.As add B (0.1～3ppm), C
0(0.2～3ppm), M
n(0.1～8ppm), M
0(0.004～0.01ppm), Zn (0.01～4ppm) etc.
3. with the natural or artificial evaporation drying of spirulina medium of above-mentioned 2. preparations, make height concentrated solution and lake water height concentrated solution, or make the SPIRULINA CULTIVATION salt powder.This is convenient to long-distance transportation, and is with low cost, makes producer's spirulina production cost reduce by 50～60%.As seen, be used for this method and can reduce the spirulina enterprise product cost significantly, increase the competitive power in its market.
Utilizing saline and alkaline lake water concentrated solution to cultivate spirulina is that a lake water is tending towards scavenging process, free from environmental pollution.And different in south, they prepare nutrient solution with well water, and pH value reaches 9～10.5; to cultivate discharging of waste liquid to outside atmosphere; cause soil and subterranean water to pollute, for many years, this has become the limiting factor of spirulina large-scale production; and produce spirulina its waste liquid is returned in the salt alkali lake in the lakeside; obtain Balance of nature, the lake water reusable edible does not cause environmental pollution; Sustainable development will be made significant contribution in the high-tech agricultural development of China's development of the West Regions.
Claims (5)
1. one kind is utilized Na
2CO
3-NaHCO
3The method that the saline and alkaline lake water concentrated solution of type is cultivated spirulina, its spy is the lake water of natural evaporation concentration is injected cultivation pool with the biological net filtration of 200～600 orders, makes its concentration adjust to 1～6 degree Beaume, adds nitrogenous source (as NaNO
31～2.5g/L, urea 0.03～0.1g/L), phosphoric acid diamines 0.3～0.7g/L, Na-EDTA0.03～0.1g/L is mixed with the nutrient solution that is suitable for growth of spirulina platensis, and in breeding process, the pH value of control solution is between 8.5～11.
2. according to the concentrated solution of claim 1. gained, be characterised in that in advance the concentrated solution more than 10 degree Beaume is injected cultivation pool, make at the bottom of the pond and pool wall evenly soaks this concentrated solution, kept 10～24 hours.
3. according to the spirulina medium of claim 1. preparation, be characterised in that adding several trace elements finely tunes it.As add B (0.1～3ppm), C
0(0.2～3ppm), M
n(0.1～8ppm), M
0(0.004～0.01ppm), Zn (0.01～4ppm) etc.
4. according to the spirulina medium of claim 1. preparation, be characterised in that this spirulina medium after natural or artificial evaporation drying, make concentrated solution or lake water concentrated solution, be used for the lakeside or be transported to the other places carrying out spirulina production.
5. according to the spirulina medium of claim 1. preparation, be characterised in that this spirulina medium or lake water concentrated solution after natural or artificial evaporation drying, make the SPIRULINA CULTIVATION pulvis, be used for the lakeside or be transported to the other places carrying out spirulina production.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021573727A CN1510123A (en) | 2002-12-23 | 2002-12-23 | Culture of spirulina by concentrated Na2 Co3-NaHCO3 saline-alakli lake water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021573727A CN1510123A (en) | 2002-12-23 | 2002-12-23 | Culture of spirulina by concentrated Na2 Co3-NaHCO3 saline-alakli lake water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1510123A true CN1510123A (en) | 2004-07-07 |
Family
ID=34236562
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA021573727A Pending CN1510123A (en) | 2002-12-23 | 2002-12-23 | Culture of spirulina by concentrated Na2 Co3-NaHCO3 saline-alakli lake water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1510123A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100876614B1 (en) | 2006-12-21 | 2009-01-08 | 한국생명공학연구원 | Injury Induction and Harvesting Method |
| CN101942391A (en) * | 2010-09-15 | 2011-01-12 | 杨苏文 | Algae culture solution and method for culturing algae |
| CN102533527A (en) * | 2011-12-18 | 2012-07-04 | 鄂托克旗隆诚化工有限责任公司 | Process and system for preparing spirulina culture solution from low-concentration alkaline lake brine |
| CN101705188B (en) * | 2009-11-30 | 2012-09-05 | 北海洼地螺旋藻产业基地有限公司 | Method for domestically culturing salt-water Spirulina |
| CN104830665A (en) * | 2015-05-28 | 2015-08-12 | 江西三达新大泽生物工程有限公司 | Method and special device for cultivating spirulina |
-
2002
- 2002-12-23 CN CNA021573727A patent/CN1510123A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100876614B1 (en) | 2006-12-21 | 2009-01-08 | 한국생명공학연구원 | Injury Induction and Harvesting Method |
| CN101705188B (en) * | 2009-11-30 | 2012-09-05 | 北海洼地螺旋藻产业基地有限公司 | Method for domestically culturing salt-water Spirulina |
| CN101942391A (en) * | 2010-09-15 | 2011-01-12 | 杨苏文 | Algae culture solution and method for culturing algae |
| CN102533527A (en) * | 2011-12-18 | 2012-07-04 | 鄂托克旗隆诚化工有限责任公司 | Process and system for preparing spirulina culture solution from low-concentration alkaline lake brine |
| CN104830665A (en) * | 2015-05-28 | 2015-08-12 | 江西三达新大泽生物工程有限公司 | Method and special device for cultivating spirulina |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |