CN102511683B - Preparation method of alga nutrient solution for prawn culture - Google Patents
Preparation method of alga nutrient solution for prawn culture Download PDFInfo
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- CN102511683B CN102511683B CN2011104251077A CN201110425107A CN102511683B CN 102511683 B CN102511683 B CN 102511683B CN 2011104251077 A CN2011104251077 A CN 2011104251077A CN 201110425107 A CN201110425107 A CN 201110425107A CN 102511683 B CN102511683 B CN 102511683B
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- nutrient solution
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 59
- 241000238557 Decapoda Species 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000000243 solution Substances 0.000 claims description 48
- 239000002002 slurry Substances 0.000 claims description 18
- 241000251468 Actinopterygii Species 0.000 claims description 16
- 238000012258 culturing Methods 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 229940088598 enzyme Drugs 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 229920006395 saturated elastomer Polymers 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- 241000228245 Aspergillus niger Species 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 108090000526 Papain Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 3
- 241000223261 Trichoderma viride Species 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- 230000007062 hydrolysis Effects 0.000 claims description 3
- 238000006460 hydrolysis reaction Methods 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 239000001103 potassium chloride Substances 0.000 claims description 3
- 235000011164 potassium chloride Nutrition 0.000 claims description 3
- 230000006798 recombination Effects 0.000 claims description 3
- 238000005215 recombination Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 239000012047 saturated solution Substances 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 238000009395 breeding Methods 0.000 abstract description 9
- 230000001488 breeding effect Effects 0.000 abstract description 9
- 241000195493 Cryptophyta Species 0.000 abstract description 8
- 230000012010 growth Effects 0.000 abstract description 7
- 230000002045 lasting effect Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 238000009313 farming Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 108010028690 Fish Proteins Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000005570 vertical transmission Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Abstract
The invention discloses a method for preparing an alga nutrient solution for the prawn culture, which is characterized in that firstly a biological active nutrient solution and an inorganic nutrient solution are respectively prepared, then the prepared biological active nutrient solution and the inorganic nutrient solution are chelated. The alga nutrient solution for the prawn culture of the invention has the advantages of rapid cultivation of beneficial algae and beneficial plankton in the water, provision of a large amount of palatable biological baits for prawns, realization of lasting building of a good water culture environment through the alga growth and breeding, culture of healthy prawns and other aquatic kinds, and realization of continuous and stable development of the aquatic breeding industry in a benign cycle.
Description
Technical field
The present invention relates to the alga nutrient solution preparing technical field, particularly a kind of alga nutrient solution preparation method for prawn culturing.
Background technology
The prawn culturing technology enters the artificial intensive intensive culture stage by half intensive culture stage, and output increases substantially.But along with development intensive, high-density breeding, cultivation water environment worsens increasingly, causes the grow shrimp quality to descend, and the prawn disease disease is frequently broken out, and disease is on the rise.Water environment degradation has become the main factor of restriction shrimp farming development.And the quality of Measures of Algae in Water Body growth and breeding directly has influence on cultivation water environment, because breaking out with the water body environment factor of shrimps in culture disease has close relationship, envirment factor is the essential condition that the shrimp body causes a disease.Therefore, control to prawn virus disease, do close shrimp except selecting not viruliferous high-quality health young shrimp, outside the vertical transmission of virosis is reduced in the source, in the prawn culturing process, the ecological balance how to keep the dynamic equilibrium of water body biotic factor (such as algae, mushroom) and physical and chemical factor (pH matter, dissolved oxygen etc.) and whole water body is most important.The various ecological factors of alga nutrient solution energy balance water body of the present invention; promote useful algae and useful planktonic growth and breeding; for prawn provides a large amount of agreeable to the taste biological feeds; impel its healthy growth; and improve water quality and pond micro-ecological environment by the growth and breeding of algae; for prawn is set up a good living environment and life condition; strengthen physique and the premunition of prawn; improve success rate and the growth rate of cultured prawn; the prawn that cultivation is healthy; and reduce shrimp farming to the pollution of paralic environment, keep the ecological balance in coastal waters, make shrimp farming sustainable and stable development in benign cycle.
Summary of the invention
The purpose of this invention is to provide a kind of alga nutrient solution preparation method for prawn culturing, to strengthen physique and the premunition of prawn, improve success rate and the growth rate of cultured prawn.
In order to realize above-mentioned purpose, a kind of alga nutrient solution preparation method for prawn culturing provided by the invention, concrete technical scheme: first biologically active nutrient solution and inorganic nutrient solution are prepared respectively, then the biologically active nutrient solution for preparing and the inorganic nutrient solution for preparing are carried out chelating, concrete preparation method and step:
1) preparation of biologically active nutrient solution:
A. first the fish material is heated after the boiling, squeeze, concentrate, be prepared into saturated fish slurry;
B. with after the marine alga drying and crushing, through the choosing of 80 mesh sieves, add water and stir the saturated marine alga slurry of preparation;
C. take fish slurry and marine alga slurry ratio as 1: 1, place in the container and stir, obtain fish slurry and marine alga pulp mixture, and adjusting pH value is 6.5-7; Cellulase and papain mixed by 1: 1 becomes complex enzyme, then in fish slurry and marine alga pulp mixture, and the ratio adding complex enzyme in 1.5 ‰, continue to stir, after 16 hours, be warming up to again 85～90 ℃ of enzymes 30 minutes of going out 50 ℃ of hydrolysis, obtain hydrolyzate;
D. hydrolyzate is regulated the pH value and added biological inoculum for 6-7 by hydrolyzate volume 1-2 ‰; Described biological inoculum is part recombination of saccharomyces cerevisiae 10%, bacillus subtilis 30%, Trichoderma viride 40%, aspergillus niger 20%; 30 ℃ of fermentations 24-48 hour, obtain the biologically active nutrient solution;
2) preparation of inorganic nutrient solution
Join by weight urea 90, MAP 10, magnesium sulfate 5, ferrous sulfate 2, zinc sulfate 2, potassium chloride 1, be dissolved in water, saturated solution is made in dissolving, obtains inorganic nutrient solution;
3) biologically active nutrient solution and inorganic nutrient solution are carried out chelating, described chelating is the ratio of biologically active nutrient solution and inorganic nutrient solution to move to reactor at 4: 1, after the stirring, 70 ℃ of chelatings 12 hours, can make the prawn culturing alga nutrient solution.
Adopt the prawn culturing alga nutrient solution of the present invention of above-mentioned measure to have: the useful algae of quickly breeding water body and useful planktonic organism, for prawn provides a large amount of agreeable to the taste biological feeds, and the growth and breeding by algae, the lasting foster environment of good water of building, cultivate healthy prawn and other aquatic products kind, make the advantage of culture fishery sustainable and stable development in benign cycle.
The specific embodiment
The specific embodiment of the invention: first biologically active nutrient solution and inorganic nutrient solution are prepared respectively, then the biologically active nutrient solution for preparing and the inorganic nutrient solution for preparing are carried out chelating, concrete preparation method and step:
1) preparation of biologically active nutrient solution:
A. first the fish material is heated after the boiling, squeeze, concentrate, be prepared into saturated fish slurry;
B. with after the marine alga drying and crushing, through the choosing of 80 mesh sieves, add water and stir the saturated marine alga slurry of preparation;
C. take fish slurry and marine alga slurry ratio as 1: 1, place in the container and stir, obtain fish slurry and marine alga pulp mixture, and adjusting pH value is 6.5-7; Cellulase and papain mixed by 1: 1 becomes complex enzyme, then in fish slurry and marine alga pulp mixture, and the ratio adding complex enzyme in 1.5 ‰, continue to stir, after 16 hours, be warming up to again 85～90 ℃ of enzymes 30 minutes of going out 50 ℃ of hydrolysis, obtain hydrolyzate;
D. hydrolyzate is regulated the pH value and added biological inoculum for 6-7 by hydrolyzate volume 1-2 ‰; Described biological inoculum is part recombination of saccharomyces cerevisiae 10%, bacillus subtilis 30%, Trichoderma viride 40%, aspergillus niger 20%; 30 ℃ of fermentations 24-48 hour, obtain the biologically active nutrient solution;
2) preparation of inorganic nutrient solution
Join by weight urea 90, MAP 10, magnesium sulfate 5, ferrous sulfate 2, zinc sulfate 2, potassium chloride 1, be dissolved in water, saturated solution is made in dissolving, obtains inorganic nutrient solution;
3) biologically active nutrient solution and inorganic nutrient solution are carried out chelating, described chelating is the ratio of biologically active nutrient solution and inorganic nutrient solution to move to reactor at 4: 1, after the stirring, 70 ℃ of chelatings 12 hours, can make the prawn culturing alga nutrient solution.
This prawn culturing alga nutrient solution is rich in the required nitrogen of algal grown breeding, phosphorus, sulphur, iron etc. are large, in, trace element, with active fish protein peptides of different nature, amino acid, algal polysaccharides, shitosan, chelating amino acids attitude element nutrient, have concurrently organic and inorganic nutrients product composition, and its composition major part is from natural materials, the naturality and the biologically active that have kept various compositions, its composition has preferably environment concordance, easily absorbed by algae and water and utilize, the quick-acting performances of existing inorganic nutrients, the lasting long-acting of organic nutrient arranged again, build good water and support environment, the prawn that cultivation is healthy and other aquatic products make culture fishery sustainable and stable development in benign cycle.
Simultaneously, the exploitation of product of the present invention is opened up a brand-new road for the application study of small fish albumen, marine alga, and being one turns waste into wealth, the philanthropic act of preserving the ecological environment.
Claims (1)
1. alga nutrient solution preparation method who is used for prawn culturing, it is characterized in that: first biologically active nutrient solution and inorganic nutrient solution are prepared respectively, then the biologically active nutrient solution for preparing and the inorganic nutrient solution for preparing are carried out chelating, concrete preparation method and step:
1) preparation of biologically active nutrient solution:
A. first the fish material is heated after the boiling, squeeze, concentrate, be prepared into saturated fish slurry;
B. with after the marine alga drying and crushing, through the choosing of 80 mesh sieves, add water and stir the saturated marine alga slurry of preparation;
C. take fish slurry and marine alga slurry ratio as 1: 1, place in the container and stir, obtain fish slurry and marine alga pulp mixture, and adjusting pH value is 6.5-7; Cellulase and papain mixed by 1: 1 becomes complex enzyme, then in fish slurry and marine alga pulp mixture, and the ratio adding complex enzyme in 1.5 ‰, continue to stir, after 16 hours, be warming up to again 85～90 ℃ of enzymes 30 minutes of going out 50 ℃ of hydrolysis, obtain hydrolyzate;
D. hydrolyzate is regulated the pH value and added biological inoculum for 6-7 by hydrolyzate volume 1-2 ‰; Described biological inoculum is part recombination of saccharomyces cerevisiae 10%, bacillus subtilis 30%, Trichoderma viride 40%, aspergillus niger 20%; 30 ℃ of fermentations 24-48 hour, obtain the biologically active nutrient solution;
2) preparation of inorganic nutrient solution
Join by weight urea 90, MAP 10, magnesium sulfate 5, ferrous sulfate 2, zinc sulfate 2, potassium chloride 1, be dissolved in water, saturated solution is made in dissolving, obtains inorganic nutrient solution;
3) biologically active nutrient solution and inorganic nutrient solution are carried out chelating, described chelating is the ratio of biologically active nutrient solution and inorganic nutrient solution to move to reactor at 4: 1, after the stirring, 70 ℃ of chelatings 12 hours, can make the prawn culturing alga nutrient solution.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011104251077A CN102511683B (en) | 2011-12-16 | 2011-12-16 | Preparation method of alga nutrient solution for prawn culture |
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| CN2011104251077A CN102511683B (en) | 2011-12-16 | 2011-12-16 | Preparation method of alga nutrient solution for prawn culture |
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| CN102511683A CN102511683A (en) | 2012-06-27 |
| CN102511683B true CN102511683B (en) | 2013-10-30 |
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| CN105265792A (en) * | 2015-10-27 | 2016-01-27 | 盐城工学院 | Alga fermentation feed additive and preparation method of alga fermentation feed additive |
| CN106588175A (en) * | 2016-11-15 | 2017-04-26 | 陈景河 | Shrimp culture ecological fertilizer water paste |
| CN112772495A (en) * | 2021-02-07 | 2021-05-11 | 渤海水产股份有限公司 | Method for cultivating pollution-free seed shrimps of prawns |
| CN112913740A (en) * | 2021-02-07 | 2021-06-08 | 渤海水产股份有限公司 | Method for transporting seed shrimps |
| CN112931332A (en) * | 2021-02-07 | 2021-06-11 | 渤海水产股份有限公司 | Method for cultivating green food prawn breeding shrimps |
| CN113749023B (en) * | 2021-10-10 | 2022-10-21 | 广东格新生物科技有限公司 | Seaweed hydrolysate produced by composite process and application thereof in shrimp culture |
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Patent Citations (6)
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