CN102511683A - Alga nutrient solution for prawn culture and preparation method thereof - Google Patents
Alga nutrient solution for prawn culture and preparation method thereof Download PDFInfo
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- CN102511683A CN102511683A CN2011104251077A CN201110425107A CN102511683A CN 102511683 A CN102511683 A CN 102511683A CN 2011104251077 A CN2011104251077 A CN 2011104251077A CN 201110425107 A CN201110425107 A CN 201110425107A CN 102511683 A CN102511683 A CN 102511683A
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 52
- 241000238557 Decapoda Species 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims description 21
- 238000012136 culture method Methods 0.000 title 1
- 241000195493 Cryptophyta Species 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 241000251468 Actinopterygii Species 0.000 claims abstract description 23
- 238000009395 breeding Methods 0.000 claims abstract description 14
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 claims abstract description 10
- 150000001413 amino acids Chemical class 0.000 claims abstract description 6
- 108010028690 Fish Proteins Proteins 0.000 claims abstract description 5
- 150000004676 glycans Chemical class 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 5
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 5
- 239000005017 polysaccharide Substances 0.000 claims abstract description 5
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 239000000126 substance Substances 0.000 claims abstract description 5
- 239000011573 trace mineral Substances 0.000 claims abstract description 5
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 5
- 238000000855 fermentation Methods 0.000 claims abstract description 4
- 230000004151 fermentation Effects 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 36
- 239000002002 slurry Substances 0.000 claims description 24
- 238000012258 culturing Methods 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 10
- 229940088598 enzyme Drugs 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 229920006395 saturated elastomer Polymers 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 4
- 241000228245 Aspergillus niger Species 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 108090000526 Papain Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 3
- 241000223261 Trichoderma viride Species 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 238000001311 chemical methods and process Methods 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- 230000007062 hydrolysis Effects 0.000 claims description 3
- 238000006460 hydrolysis reaction Methods 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 239000001103 potassium chloride Substances 0.000 claims description 3
- 235000011164 potassium chloride Nutrition 0.000 claims description 3
- 230000006798 recombination Effects 0.000 claims description 3
- 238000005215 recombination Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 239000012047 saturated solution Substances 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000002045 lasting effect Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract 2
- 229920001661 Chitosan Polymers 0.000 abstract 1
- 235000006180 nutrition needs Nutrition 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 238000009313 farming Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000005570 vertical transmission Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Cultivation Of Seaweed (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an alga nutrient solution for the prawn culture. The special alga nutrient solution for the prawn culture is prepared by selecting small deep sea fish and algae as main raw materials, processing the main raw materials through a physicochemical process combined with an enzyme engineering technology and a biological fermentation technology to obtain a biological nutrient solution rich in different property nutrients of active fish protein peptides, amino acids, alga polysaccharides and chitosans, and blend-chelating major, medium and trace elements according to physical and chemical factors of culture water and nutrition needs of the growth and the breeding of the algae. The alga nutrient solution for the prawn culture of the invention has the advantages of rapid cultivation of beneficial algae and beneficial plankton in the water, provision of a large amount of palatable biological baits for prawns, realization of lasting building of a good water culture environment through the alga growth and breeding, culture of healthy prawns and other aquatic kinds, and realization of continuous and stable development of the aquatic breeding industry in a benign cycle.
Description
Technical field
The present invention relates to algae nutrient solution preparing technical field, particularly a kind of algae nutrient solution and preparation method who is used for prawn culturing.
Background technology
The prawn culturing technology gets into the artificial intensive intensive culture stage by half intensive culture stage, and output increases substantially.But along with the development of intensification, high-density breeding, cultivation water environment worsens day by day, causes the grow shrimp quality to descend, and the prawn disease disease is frequently broken out, and disease is on the rise.Water environment degradation has become the main factor of restriction shrimp farming development.And the quality of algal grown breeding directly has influence on cultivation water environment in the water body, because breaking out with the water body environment factor of shrimps in culture disease has confidential relation, envirment factor is the essential condition that the shrimp body causes a disease.Therefore; Control to prawn virus disease; Do close shrimp except selecting not viruliferous high-quality health young shrimp for use; Outside the vertical transmission of virosis was reduced in the source, in the prawn culturing process, the ecological balance of dynamic equilibrium and whole water body how to keep water body biotic factor (like algae, mushroom) and physical and chemical factor (PH matter, dissolved oxygen etc.) was most important.The various ecological factors of algae nutrition fluid power equilibrium water body of the present invention promote useful algae and useful planktonic growth and breeding, for prawn provides a large amount of agreeable to the taste biological feeds; Impel its healthy growth, and improve water quality and pond micro-ecological environment, for prawn is set up good living environment and life condition through the algae grows breeding; Strengthen physique and the premunition of prawn; Improve the success rate and the growth rate of cultured prawn, culture healthy prawn, and reduce the pollution of shrimp farming paralic environment; Keep the ecological balance in coastal waters, make shrimp farming sustainable and stable development in benign cycle.
Summary of the invention
The purpose of this invention is to provide a kind of algae nutrient solution and preparation method who is used for prawn culturing,, improve the success rate and the growth rate of cultured prawn to strengthen physique and the premunition of prawn.
In order to realize above-mentioned purpose; A kind of algae nutrient solution and preparation method who is used for prawn culturing provided by the invention; Concrete technical scheme: selecting deep-sea small fish, marine alga for use is primary raw material; Handle back desmoenzyme engineering, biofermentation technique through the physics and chemistry method, obtain being rich in the biological nutrient liquid of active fish protein peptides of different nature, amino acid, algal polysaccharides, shitosan class nutrient, breed nutritional need according to breeding water body physical and chemical factor and algal grown; Big or middle, the trace element of allotment chelating is made into the special-purpose algae nutrient solution of prawn culturing;
Concrete preparation method and step:
One. the preparation of biologically active nutrient solution
A) after first fish material is heated boiling, squeeze, concentrate, be prepared into saturated fish slurry;
B),, add water and stir the saturated marine alga slurry of preparation through the choosing of 80 mesh sieves with after the marine alga drying and crushing;
C) be 1: 1 with fish slurry with marine alga slurry ratio, place to hold in the tool and stir, obtain fish slurry and marine alga pulp mixture, and the adjusting pH value is 6.5-7; Add complex enzyme by 1.5 ‰ of fish slurry and marine alga pulp mixture then; Described complex enzyme is to obtain by 1: 1 mixed cellulase and papain; Continue to stir, 50 ℃ of hydrolysis after 16 hours, be warming up to 85～90 ℃ of enzymes 30 minutes of going out again, obtain hydrolyzate;
D) hydrolyzate being regulated pH value is that 6-7 adds biological inoculum by hydrolyzate volume 1-2 ‰; Described biological inoculum is part recombination of saccharomyces cerevisiae 10%, bacillus subtilis 30%, Trichoderma viride 40%, aspergillus niger 20%; 30 ℃ of fermentations 24-48 hour, obtain the biologically active nutrient solution;
Two. the preparation of inorganic nutrient solution
Compare by 100 parts of reprovisions: urea 90, MAP 10, magnesium sulfate 5, ferrous sulfate 2, zinc sulfate 2, potassium chloride 1, be dissolved in water, saturated solution is processed in dissolving, obtains inorganic nutrient solution;
Three. biologically active nutrient solution and inorganic nutrient solution are carried out chelating; Described chelating: is nutrient solution to move to agitated reactor at 4: 1 in the ratio of fish slurry and marine alga slurry, after the stirring, 70 ℃ of chelatings 12 hours, can make prawn culturing algae nutrient solution.
Adopt the prawn culturing algae nutrient solution of the present invention of above-mentioned measure to have: useful algae of quickly breeding water body and useful planktonic organism; For prawn provides a large amount of agreeable to the taste biological feeds; And through the algae grows breeding; The lasting foster environment of good water of building is cultured healthy prawn and other aquatic products kind, makes the advantage of culture fishery sustainable and stable development in benign cycle.
The specific embodiment
The specific embodiment of the invention: selecting deep-sea small fish, marine alga for use is primary raw material; Desmoenzyme engineering, biofermentation technique after the physics and chemistry method is handled; Obtain being rich in the biological nutrient liquid of active fish protein peptides of different nature, amino acid, algal polysaccharides, shitosan class nutrient; According to breeding water body physical and chemical factor and algal grown breeding nutritional need, big or middle, the trace element of allotment chelating is made into the special-purpose algae nutrient solution of prawn culturing.
Concrete preparation method and step:
One. the preparation of biologically active nutrient solution
A) after first fish material is heated boiling, squeeze, concentrate, be prepared into saturated fish slurry;
B),, add water and stir the saturated marine alga slurry of preparation through the choosing of 80 mesh sieves with after the marine alga drying and crushing;
C) be 1: 1 with fish slurry with marine alga slurry ratio, place to hold in the tool and stir, obtain fish slurry and marine alga pulp mixture, and the adjusting pH value is 6.5-7; Add complex enzyme by 1.5 ‰ of fish slurry and marine alga pulp mixture then; Described complex enzyme is to obtain by 1: 1 mixed cellulase and papain; Continue to stir, 50 ℃ of hydrolysis after 16 hours, be warming up to 85～90 ℃ of enzymes 30 minutes of going out again, obtain hydrolyzate;
D) hydrolyzate being regulated pH value is that 6-7 adds biological inoculum by hydrolyzate volume 1-2 ‰; Described biological inoculum is part recombination of saccharomyces cerevisiae 10%, bacillus subtilis 30%, Trichoderma viride 40%, aspergillus niger 20%; 30 ℃ of fermentations 24-48 hour, obtain the biologically active nutrient solution;
Two. the preparation of inorganic nutrient solution
Compare by 100 parts of reprovisions: urea 90, MAP 10, magnesium sulfate 5, ferrous sulfate 2, zinc sulfate 2, potassium chloride 1, be dissolved in water, saturated solution is processed in dissolving, obtains inorganic nutrient solution;
Three. biologically active nutrient solution and inorganic nutrient solution are carried out chelating; Described chelating: is nutrient solution to move to agitated reactor at 4: 1 in the ratio of fish slurry and marine alga slurry, after the stirring, 70 ℃ of chelatings 12 hours, can make prawn culturing algae nutrient solution.
This prawn culturing algae nutrient solution is rich in big or middle, trace elements such as the required nitrogen of algal grown breeding, phosphorus, sulphur, iron; With active fish protein peptides of different nature, amino acid, algal polysaccharides, shitosan, chelating amino acids attitude element nutrient, have concurrently organic and inorganic nutrients article composition, and its composition major part is from natural materials; The naturality and the biologically active that have kept various compositions; Its composition has environment concordance preferably, is prone to absorbed and utilization the quick-acting performances of existing inorganic nutrients by algae and water; The lasting long-acting of organic nutrient arranged again; Build good water and support environment, culture healthy prawn and other aquatic products, make culture fishery sustainable and stable development in benign cycle.
Simultaneously, Products Development of the present invention is for a brand-new road is opened up in the application study of small fish fish albumen, marine alga.It is a philanthropic act that turns waste into wealth, preserves the ecological environment.
Claims (2)
1. algae nutrient solution that is used for prawn culturing; It is characterized in that: selecting deep-sea small fish, marine alga for use is primary raw material; Handle back desmoenzyme engineering, biofermentation technique through the physics and chemistry method, obtain being rich in the biological nutrient liquid of active fish protein peptides of different nature, amino acid, algal polysaccharides, shitosan class nutrient, breed nutritional need according to breeding water body physical and chemical factor and algal grown; Big or middle, the trace element of allotment chelating is made into the special-purpose algae nutrient solution of prawn culturing.
2. a kind of preparation method who is used for the algae nutrient solution of prawn culturing according to claim 1 is characterized in that:
Concrete preparation method and step:
One. the preparation of biologically active nutrient solution
A) after first fish material is heated boiling, squeeze, concentrate, be prepared into saturated fish slurry;
B),, add water and stir the saturated marine alga slurry of preparation through the choosing of 80 mesh sieves with after the marine alga drying and crushing;
C) be 1: 1 with fish slurry with marine alga slurry ratio, place to hold in the tool and stir, obtain fish slurry and marine alga pulp mixture, and the adjusting pH value is 6.5-7; Add complex enzyme by 1.5 ‰ of fish slurry and marine alga pulp mixture then; Described complex enzyme is to obtain by 1: 1 mixed cellulase and papain; Continue to stir, 50 ℃ of hydrolysis after 16 hours, be warming up to 85～90 ℃ of enzymes 30 minutes of going out again, obtain hydrolyzate;
D) hydrolyzate being regulated pH value is that 6-7 adds biological inoculum by hydrolyzate volume 1-2 ‰; Described biological inoculum is part recombination of saccharomyces cerevisiae 10%, bacillus subtilis 30%, Trichoderma viride 40%, aspergillus niger 20%; 30 ℃ of fermentations 24-48 hour, obtain the biologically active nutrient solution;
Two. the preparation of inorganic nutrient solution
Compare by 100 parts of reprovisions: urea 90, MAP 10, magnesium sulfate 5, ferrous sulfate 2, zinc sulfate 2, potassium chloride 1, be dissolved in water, saturated solution is processed in dissolving, obtains inorganic nutrient solution;
Three. biologically active nutrient solution and inorganic nutrient solution are carried out chelating; Described chelating: is nutrient solution to move to agitated reactor at 4: 1 in the ratio of fish slurry and marine alga slurry, after the stirring, 70 ℃ of chelatings 12 hours, can make prawn culturing algae nutrient solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011104251077A CN102511683B (en) | 2011-12-16 | 2011-12-16 | Preparation method of alga nutrient solution for prawn culture |
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| CN2011104251077A CN102511683B (en) | 2011-12-16 | 2011-12-16 | Preparation method of alga nutrient solution for prawn culture |
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| CN102511683A true CN102511683A (en) | 2012-06-27 |
| CN102511683B CN102511683B (en) | 2013-10-30 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105265792A (en) * | 2015-10-27 | 2016-01-27 | 盐城工学院 | Alga fermentation feed additive and preparation method of alga fermentation feed additive |
| CN106588175A (en) * | 2016-11-15 | 2017-04-26 | 陈景河 | Shrimp culture ecological fertilizer water paste |
| CN112772495A (en) * | 2021-02-07 | 2021-05-11 | 渤海水产股份有限公司 | Method for cultivating pollution-free seed shrimps of prawns |
| CN112913740A (en) * | 2021-02-07 | 2021-06-08 | 渤海水产股份有限公司 | Method for transporting seed shrimps |
| CN112931332A (en) * | 2021-02-07 | 2021-06-11 | 渤海水产股份有限公司 | Method for cultivating green food prawn breeding shrimps |
| CN113749023A (en) * | 2021-10-10 | 2021-12-07 | 广东格新生物科技有限公司 | Seaweed hydrolysate produced by composite process and application thereof in shrimp culture |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112772495A (en) * | 2021-02-07 | 2021-05-11 | 渤海水产股份有限公司 | Method for cultivating pollution-free seed shrimps of prawns |
| CN112913740A (en) * | 2021-02-07 | 2021-06-08 | 渤海水产股份有限公司 | Method for transporting seed shrimps |
| CN112931332A (en) * | 2021-02-07 | 2021-06-11 | 渤海水产股份有限公司 | Method for cultivating green food prawn breeding shrimps |
| CN113749023A (en) * | 2021-10-10 | 2021-12-07 | 广东格新生物科技有限公司 | Seaweed hydrolysate produced by composite process and application thereof in shrimp culture |
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| CN102511683B (en) | 2013-10-30 |
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Application publication date: 20120627 Assignee: Beihai Fulin green biological fertilizer Co., Ltd. Assignor: Chen Jinghe Contract record no.: 2014450000002 Denomination of invention: Preparation method of alga nutrient solution for prawn culture Granted publication date: 20131030 License type: Exclusive License Record date: 20140120 |
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Effective date of registration: 20170510 Address after: 536100 the Guangxi Zhuang Autonomous Region Beihai Hepu County Industrial Park Patentee after: Beihai Fulin green biological fertilizer Co., Ltd. Address before: 536000, the Guangxi Zhuang Autonomous Region Beihai Evergreen Road East No. 52 Forestry Bureau flint company Patentee before: Chen Jinghe |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131030 Termination date: 20201216 |