CN1594232A - Composite amino acid ecological nutritious agent - Google Patents
Composite amino acid ecological nutritious agent Download PDFInfo
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- CN1594232A CN1594232A CNA200410013413XA CN200410013413A CN1594232A CN 1594232 A CN1594232 A CN 1594232A CN A200410013413X A CNA200410013413X A CN A200410013413XA CN 200410013413 A CN200410013413 A CN 200410013413A CN 1594232 A CN1594232 A CN 1594232A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The invention provides a composite amino acid ecological nutritious agent, which comprises sea weed fermentation liquor, fermentation liquor of animal leftover bits and pieces, trace elements, and biological bacterium. The functions of the agent include stimulating growth, suppressing pathogenic agent and reinforcing immunization.
Description
Technical field
The present invention relates to the agent of a kind of aminoacids complex ecoalimental, can be used to spray farm crop and promote growth, also can be used as fodder additives, be applied to the breed of land and water animals such as pig, chicken, duck, goose, shrimp, crab.
Background technology
Present being seen liquid fertilizer has numerous species, and conclusion is got up, and mainly contains following a few class: the one, and inorganic fertilizer, promptly nitrogen phosphorus potassium adding trace element and complex compound are made solution; The 2nd, fertilizer, the plant-growth regulator that promptly driven plant extract obtains; The 3rd, mix fertilizer, be that fertilizer is added inorganic microelement.The liquid fertilizer that adds tethelin is also arranged in addition, add full-service fluid fertilizer of agricultural chemicals or preventing disease and pest material or the like in addition.But in the present being seen liquid fertilizer, part also comes with some shortcomings, the cost height that has, consumption is big, the poorly water-soluble that has, the element mismatch that has is difficult for by plant absorbing, and the hormones liquid fertilizer that has can make plant produce taking off phenomenon, output had improved after the liquid fertilizer of considerable part sprayed in addition, but its quality is not as people's will, and the same with to fertilize crop quality, fruit is big and sweetless, dish is strong and not tender, mouthfeel is poor, so most liquid fertilizer is eliminated in use for some time gradually, has only minority can continue to be existed.
Present being seen animal rearing nutritional additive also has multiple, major part is to cooperate with amino acid, protein and trace element, the independent adding that also has has the marine alga that promotes the growth effect to animal, it is not only beneficial to animal that but many people are not fully recognized that some marine organisms, and promote growth that better action is also arranged to plant.
We are by retrieval, it is as follows to find some scientific and technical literatures fertile about liquid and the animal rearing nutritional additive: 1, Chinese patent＜application number〉02116711＜denomination of invention〉manufacture method＜applicant of fermented organic amino acid fertilizer〉Yuan Denghui, Hu Maosen, No. 10＜the digest in Luobulin Fortress road, Tibet Autonomous Region Lhasa〉a kind of fermented organic amino acid fertilizer, it comprises that 5-15%'s contains potassium agricultural amino acid powder, 60-80% by domestic sludge, animal excreta, organic fermentation fermentation material that beer groove and the plant cake and the peat composed of rotten mosses etc. are made for raw material, the inorganic fertilizer that contains trace element of 15-40%.Utilization rate of fertilizer height, the fertilizer efficiency speed that has this fermented organic amino acid fertilizer slowly has both, improvement and vegetable soil, improve advantage such as crop quality low price.Be specially adapted to big cess, the Shan crop of the ecological agriculture.2, Chinese patent＜application number〉01135137＜denomination of invention〉extraction process＜applicant of seaweed plant growth hormone〉Institute of Oceanology of the Chinese Academy of Sciences, No. 7, Qingdao of Shandong province South Sea road,＜digest〉a kind of extraction process of seaweed plant growth hormone, belong to the preparation of marine alga biochemical substances, be used for extracting trimethyl-glycine and analogue thereof from marine alga.It is characterized in that this technology comprises: washing fresh marine alga impurity elimination chopping, adding dehydrated alcohol to alcohol concn is 65～75% and making beating is filtered, extraction, evaporation is anhydrated and ethanol and pigment, the centrifugal salt of separating out get crude extract, add six steps such as excessive Reinecke's salt precipitation.It is simple to have technology, and extraction cost is low, and fully the use of Natural fertilizers is strengthened and promoted to the comprehensive exploitation rich seaweed resources, improves advantages such as crop yield and quality.3, Chinese patent＜application number〉01115067＜denomination of invention〉natural and environment-protecting type organic fertilizer and preparation method thereof＜applicant〉Xu Dongyuan, Qingdao of Shandong province volt Unit three, No. 5 building, the village, Longshan 403＜digest〉a kind of native annulus possesses organic fertilizer and preparation method thereof, belong to the preparation of agricultural fertilizer, be mainly used in growth, volume increase such as rural area, booth promotion wheat, corn, paddy rice, peanut and various gourd, fruit and vegetables.It is characterized in that the concentrated solution that this fertilizer is made through the soaking fermentation distillation by dry sweet potato, marine alga, cereal chaff slag, the silkworm excrement of geometric ratio, geometric ratio is mixed formation mutually with mother liquid of red orchid and glucose again.Its technology comprise weigh stir evenly to pulverize that jar, the fermentation of soaking, heat up, distillation concentrate, three liquid are mixed into jar, intensification is lowered the temperature step such as vacuumizes.It is simple to have Recipe, and pure natural substance is nutritious, and purposes is wide, increases production advantages such as disease-resistant.4, Chinese patent＜application number〉00132255＜denomination of invention〉novel sea biological multielement fertilizer and preparation method thereof＜applicant〉Gu Guangchao, No. 18 building, LiuFangNanLi, Chaoyang District, BeiJing City No. 301＜contriver in Unit 1〉Gu Guangchao＜digest〉the invention discloses a kind of novel sea biological multielement fertilizer and preparation method thereof, this compound manure comprises the required nitrogen of plant growth, phosphorus, potassium, calcium, magnesium, sulphur, silicon, boron, copper, iron, manganese, molybdenum, zinc, amino acid, humic acids, organic substance, also contains the residual body of marine alga and marine animal.Product of the present invention not only contains organic component and also contains inorganic composition, can provide comprehensive nutritive element for crop.Use this product and can solve soil compaction, and make increasing crop yield more than 20%, more particularly can improve quality of agricultural product.5, Chinese patent＜application number〉99103545＜denomination of invention〉be used to promote the production method of the seaweed extracted liquor of plant-growth, resulting seaweed extracted liquor, and compound manure＜applicant〉American Golden-Autumn Co.＜address〉Maryland, USA＜contriver〉the side Wu Qiu that shares the same light threads, Li Genshen＜digest〉the invention provides a kind of production method of seaweed extracted liquor, it comprises: (1) carries out alkaline hydrolysis to the refrigerated marine alga at normal pressure, 80-100 ℃ under the condition of pH=8-10; (2) at normal pressure, room temperature under the condition of pH=1.7-3.2, is carried out acidolysis; (3) merging filtrate obtains kelp extract of the present invention.The present invention also provides a kind of compound manure and the application in coordinate plant growth thereof.6, Chinese patent＜application number〉98101040＜denomination of invention a kind of production method＜applicant of functional marine alga fertilizer the clean stone mirror of soup I, No. 101＜the digest in Unit 2, the 93rd buildings, red flag village, seafront, Beijing〉a kind of production method of functional marine alga fertilizer, it is that employing marine organisms brown alga is a main raw material, with potassium hydroxide is digestive pharmaceutical, its digestion temperature is 70 ℃～100 ℃, can make nutritive substance contained in the brown alga, trace element and spontaneous growth are regulated material, become for the benefit of by the soluble status of plant absorbing, be equipped with the hydrolyzed solution of obtaining property fish more, not only regulate the pH value of extracting solution, increased nutritive substance such as amino acid in the extracting solution simultaneously.On the technology of strengthening mineral matter nutritional, can make the above positively charged ion of brown alga extracting solution and divalence directly form complex salt, greatly reduce production cost.This marine alga fertilizer has liquid, soluble powder and three kinds of formulations of pulvis, can increase the nutrition of plant, has the effect of diseases prevention, expelling parasite, volume increase simultaneously, is nutrition agent, viral inhibitors, growth regulator and the soil conditioner of plant.7, Chinese patent＜application number〉94111617＜denomination of invention〉production technique＜applicant of farm manure〉Zhou Jialong, No. 1 building 4-4＜digest of the street All-China Federation of Taiwan Compatriots of Jiangjin, Sichuan Province city Nanan〉the present invention relates to the trace element fertilizer field, not only has the function that promotes plant-growth, also has the effect of kill pests, germ, both can foliage-spray, also can be used as base fertilizer, topdress.The present invention is aided with pyrethrin based on nitrogen, phosphorus, potash fertilizer and trace element, owing to added sodium alginate uniquely, has improved drug effect and fertilizer efficiency.8, Chinese patent＜application number〉02115611＜notification number〉1372816＜denomination of invention〉livestock and poultry nutrients additive for biological feed and production method＜applicant thereof〉Guangxi Research Institute of Chemical and Biological Technologies＜digest〉the invention belongs to a kind of livestock and poultry nutrients additive for biological feed, it comprises Methionin bacterial classification tunning and genus bacillus, microorganisms such as photosynthetic bacteria, and be added with dregs of beans, plant amylum and trace element, this additive amino acid primary liquid of biological fermentation, keep more complete aminoacid pattern, can make the biological value of animal higher, can also decompose the ammonia-state nitrogen that produces in the livestock and poultry body, nitrous acid, objectionable impuritiess such as hydrogen sulfide, reduce the stink of ight soil, reduce the pollution of farm animal excrement to environment, simultaneously still fine thalline high protein own, and contain abundant methionine(Met), therefore have one's own knack improving on the animal immunizing power, improving protein utilization, improve the growth of animals or poultry situation, aspects such as enhancing immunologic function of livestock and birds are better than similar conventional feed.9, Chinese patent＜application number〉01136594＜denomination of invention〉a kind of health care feedstuff additive for animals＜applicant〉Beijing Zhongke Shenlan Science ﹠ Technology Development Co. Ltd., No. 12＜the digest in B, Zhongguancun Road, Haidian District, Beijing City〉a kind of health care feedstuff additive for animals, by mannooligo saccharide, galactomannan-oligosaccharide, wood oligose, the combination 6-15% of alga oligosaccharide and oligochitosan functional oligose (oligosaccharides), trimethyl-glycine 6-12%, garlicin 1-5%, mixings such as Semen Maydis powder 23-60% and stone flour 8-64% are made, wherein mannooligo saccharide, galactomannan-oligosaccharide, wood oligose, alga oligosaccharide, the ratio of oligochitosan is 0-3: 6-8: 0-2: 0-2: 0-1.Steady chemical structure of the present invention, be convenient to processing and store, do not exist incompatibility, comprehensive cooperation by forage components such as energy, albumen, mineral, amino acid and VITAMIN, form the animal health feed, under the situation that antibiotic-free adds, can strengthen the immune resistivity of the object of feeding, when improving production performance, solved the antibiotic remains problem of animal product, thereby won higher culture benefit.10, Chinese patent＜application number〉94110350＜denomination of invention〉the aminoacids complex huge legendary turtle closes the preparation method＜applicant of salt and protolysate〉and Dalian aquatic product Academy, Daliang City, Liaoning Province black stone reef＜digest〉the present invention relates to a kind of preparation method and proteolysis method of compound amino-acid chelating salt.Compound amino-acid chelating salt is mainly as the soil improvement agent of trace mineral supplement in the animal-feed and short plant growth.Compound amino-acid chelating salt preparation method's of the present invention technical measures are to add nutrition carrier sorbent materials such as wheat bran, soya-bean cake in chelatropic reaction liquid, both simplified concentration technology link, the saving energy in the past, and made product have both water-soluble and non-water-soluble chelate and amino acid and small-molecular peptides again; The proteolysis method is to adopt the medium of the mixed solution of the vitriol oil or concentrated hydrochloric acid and phosphoric acid as proteolysis, its effect not only can be accelerated proteolysis reaction speed, and can make and be rich in growth of animal in the chelating salt, grow necessary soluble phosphate.
Though above relevant liquid fertilizer of recognizing or animal rearing nutritional additive have been mentioned adding seaweed extracted liquor or strain fermentation product respectively, handle just some simple methods, the product nutrient that obtains is comprehensive inadequately.
Processing for tankage also only retrieves some simple methods from open source literature, and as high temperature and high pressure method, alkali hydrolysis method, the product that obtains also has only meat meal tankage, hydrolyzed feather meal, blood meal etc. mostly.The imperfect manufacture of these methods has needs high-temperature high-pressure apparatus, and the nutritive ingredient that has is not comprehensive, and the animal blood meal that has, meat meal tankage be poison in spite of illness, and causes the diffusion of labour feelings.
Summary of the invention
The purpose of this invention is to provide the aminoacids complex ecoalimental agent that can be applied to plant and animal simultaneously, when this aminoacids complex ecoalimental agent is used to spray farm crop, can promote growth, make crop can increase output, can improve quality again; When being applied to the breed of land and water animals such as pig, chicken, duck, goose, shrimp, crab, have characteristics such as nontoxic, nuisanceless, pollution-free, no hormone, can promote growth, can also suppress pathogenic agent, strengthening immunity.
The inventor be engaged in agricultural production and to amino acid research and production process in discovery marine alga and tankage are used microbial fermentation separately, mix some amino acid after the mixing, add trace element again, add the biological bacteria Secondary Fermentation again, can prepare the agent of aminoacids complex ecoalimental, the agent of this aminoacids complex ecoalimental can be directly as fodder additives and the plant growth promoter of animal, if add the conventional element of some nitrogen phosphorus potassium again, just can make the comprehensive leaf leaven of nutrition.
The composition and the mass content of aminoacids complex ecoalimental of the present invention agent are as follows:
Marine alga fermented liquid 20-30%
Tankage fermented liquid 20-45%
Biological bacteria: 1-5%
Trace element 0.5-1%
All the other are water.
Above-mentioned marine alga fermented liquid is the red algae that will grow in the ocean, brown alga, plants such as green alga, these plants can be the sea-tangles that eats, laver, dulse, wakame, the belt marine alga, hundred lavers, Bangiales, or the tankage of these marine algas are (because the marine alga that has is the food raw material of the special plantation of people, in the course of processing, to abandon the rim charge of many irregularities), also can adopt sea grass that anorexia uses etc. to carry out the product of anaerobically fermenting, concrete grammar is that the per kilogram marine alga of will collect adds the 8-20 kg of water, add 1-5% cellulase and milk-acid bacteria and zymic mixture again by the marine alga total amount, make its condition at 20-60 ℃, ferment in jar fermenter, marine alga promptly is decomposed after 1-10 days.
More than said cellulase identical with milk-acid bacteria and zymic part by weight, also can add by the situation of observing material, many as seaweed fiber, then add the plain enzyme of some fibre, as the starch content height, then add yeast.
More than said yeast comprise solid-state, semi-solid state, liquidly cultivate the concentrating of microbial inoculum and they, dilution, absorption, spraying drying, vacuum-drying agent.
More than said milk-acid bacteria comprise the concentrating of semi-solid state, the liquid microbial inoculum of cultivating and they, dilution, absorption, vacuum-drying agent.
Above-mentioned tankage fermented liquid is meant that tankage such as the bone that will abandon in pig, ox, sheep, chicken, duck, goose, the fish course of processing, blood, liver, intestines, tire, tripe, fish scale, the fish gill smash the material that the back obtains with microbial fermentation, its fermenting process is to add the 8-20 kg of water by animal tankage per kilogram, the papoid or the subtilisin that add 1-5% again by animal tankage total amount, or stomach en-, the perhaps mixture of these three kinds of enzymes, make its condition at 20-60 ℃, in jar fermenter, ferment, obtain the tankage fermented liquid after 1-10 days.
Above-mentioned trace element comprises mineral compound, the organic compound of iron, copper, zinc, calcium, selenium, manganese, as Ferrous Gluconate, copper sulfate, zinc sulfate, grape calcium gluconate, manganous sulfate, sodium selenate etc.Its additional proportion adds according to the demand of farm crop and animal, as when being used for animal, can suitably add iron, zinc, calcium, selenium, when being used for plant, can add iron, copper, zinc, manganese etc., also can add the fertilizer commonly used of nitrogenous, phosphorus, potassium.
Above-mentioned biological bacteria is a photosynthetic bacteria, milk-acid bacteria, yeast, and every kind of bacterium of its weight content is 1: 1: 1.
The production method of aminoacids complex ecoalimental of the present invention agent is to prepare earlier marine alga fermented liquid and tankage fermented liquid respectively, adds trace element earlier, then it is mixed at normal temperatures, add biological bacteria again, reaction is 1-2 days in container, refilters, obtain product, bottling can be dispatched from the factory.
Why can aminoacids complex ecoalimental of the present invention agent also can be used as plant growth promoter directly as the fodder additives of animal? chief reason have following some:
1, the singularity of the living environment of marine alga has determined special chemical constitution and structure in the marine alga body, most of marine alga be grown in benthos be not subjected to artificially-contaminated plant, wherein contain a large amount of carbohydrate, wherein the most important thing is sugar, usually account for more than 50% of marine alga dry-matter, these sugars the most important thing is Sargassum polysaccharides, Sargassum polysaccharides is a most important moiety in the marine alga, according to it in the intravital distribution situation of algae, can be divided three classes: outermost layer is a cell wall polysaccharides, is the crystallization of microfibre shape more; Next is to be filled in the mucopolysaccharide that cell wall polysaccharides is asked, mostly is unbodied glue; The third is to be present in intracellular storage polysaccharide.If isolated polysaccharide kind from marine alga mainly contains Mierocrystalline cellulose and hemicellulose (all belonging to cell wall polysaccharides), algin, agar-agar, fucoidin, agarose and sulphur fine jade sugar (all belonging to the cell mucopolysaccharide), marine alga starch (storage polysaccharide) etc.Algin, agar-agar, fucoidin, agarose and the sulphur fine jade sugar etc. that wherein constitute cell and polysaccharide are the peculiar carbohydrates of marine alga, also be to produce and most widely used carbohydrate in the seaweed industry, trehalose has the protein denaturation of preventing or says the effect that makes it stable, can fully use trehalose in fields such as containing protein food product and various enzyme system punishment, pharmaceuticals.Marine alga contains crude protein 8-15% in addition, robust fibre is more than 10%, crude fat 1-3%, contain and be fit to animal and plant grow necessary nitrogen-free extract and iron, calcium, copper, magnesium, potassium, sodium, cobalt, iodine, selenium, phosphorus etc. are normal, trace element, contain vitamin A, vitamins C, vitamin-E, the VITMAIN B1 Wei ShengsuB2, multivitamins such as vitamin B12, contain pigment and many active substances that can promote growth of animal, also contain a large amount of alginic acids, starch, nutritive substances such as N.F,USP MANNITOL, these nutritive substances can partly satisfy domestic animal, the nutritional need of poultry promotes growth and the weightening finish of livestock and poultry.If by fermentation, this solution also is easy to be promoted its growth by plant absorbing.
Trehalose has had bibliographical information to the effect of plant, the foreign literature that has is pointed out, proved the existence of Trehalose Metabolism in the higher plant body, but its content is quite low in most of plants, study its physiological function under adverse environmental factor so can utilize the trehalose of external source to plant, utilize 0.1% aqueous trehalose immersion rice paddy seed, after 2 ℃ and 6 ℃ of subzero treatment, the cell ionogen rate of release of rice seedling significantly reduces, diastatic activity and seedling soluble sugar content obviously improve, repair ability to cold damage also improves, and treatment temp is low more, and the relative effect of trehalose is just obvious more, the mung bean seedlings that the marine alga list is handled, under dry and freezing conditions, the activity on its plasma membrane significantly improves.The soybean of accumulation trehalose in the root nodule, its blade relative water content is higher and drought tolerance is also strong under the drought stress, with the pretreated wheat of the trehalose of different concns, at the KCl growth from solution, its cell ionogen rate of release significantly reduces. and trehalose can prevent chlorophyllous degraded, keep higher root system vigor, improve the accumulation and the plant growth rate of the dry-matter under the salt condition.Bibliographical information is arranged, marine alga nuclear energy reduces the restraining effect of NaCl to young rice seedlings growth, prevent chlorophyllous degraded, change the distribution of ion different sites in the rice plants body, make that the salt accumulation volume reduces greatly in the blade, and with leaf slightly intermediate ion increase arranged slightly, promote the formation of seedling root ventilating tissue.
Trehalose to the effect of animal except higher nutritive value is arranged, the activity that also has antibacterium and fungi, because of it contains multiple germ resistance composition, wherein terpenoid has the activity of inhibition to multiple bacterium such as staphylococcus, mycobacterium and huge bud pole bacterium, have moderate to suppress active to fungi, bromine phenolic compound and fatty compounds have anti-microbial activity to gram-positive microorganism and negative bacterium etc.; Polysaccharide has certain bacteriostatic activity to trichophyton gypseum, little armful of dermatophytes such as daughter bacteria, acrothesium floccosum and Candida albicans of gypsum sample.So trehalose can improve the trophic structure of feed effectively as raising section's additive, improve efficiency of feed utilization, improve the animal product quality, improve the disease-resistant and anti-stress ability of animal etc., production performance and fecundity that poultry is closed have obvious facilitation.
2, marine alga-anaerobic fermented liquid also has the effect of disease-resistant, insect-prevention, this effect is proved by a large amount of experiments that anaerobic fermented liquid plays the effect of prevention and elimination of disease and pests by the stem that directly sprays crop, dried, leaf, can also be as seed-soaking liquid, thus reach the purpose of prevention and elimination of disease and pests.Confirmed that now anaerobic fermented liquid is to various farm crop such as grain, cash crop, vegetables, mosaic disease in 17 kinds of crop species such as fruit, nearly 30 kinds of diseases such as blight have good prevention effect, and can prevent and treat wheat, corn, the aphid of 13 kinds of crops such as paddy rice, 14 kinds of insect pests such as bollworm, because application test is still continuing widely, can predict the effect this and more also of the preventing disease pest control of anaerobic digestion residue, someone tests, marine alga can be used as antibacterials, some marine algas have significantly antiviral and anti-microbial effect. and the someone investigates 29 kinds of Pacific Ocean red algaes, find they extract 99% can suppress viral proliferation effectively, the anti-microbial activity of some Seaweed Extracts surpasses microbiotic such as penicillin.
3, the nitrogen of marine alga, phosphorus content are less. but extraordinary abundant of the content of inorganic elementss such as potassium.In addition, most suitable living kelp also contains some tethelin, as phytokinin isoreactivity material.The agronomist of countries such as South Africa, Britain, Norway selects for use the living marine alga of some large-scale speed to extract and concentrates its effective constituent, makes valuable readily avaiable fertilizer.It is reported that this liquid seaweed manure is applied to cereal, fruit, vegetables, flowers and other crops, to promoting the growth of crop, effects such as improvement fruit quality are splendid, and winter resistance, the inhibition disease and pest that strengthens crop also had unique usefulness.There are South Africa, Norway, Britain, France, New Zealand etc. in the country that at present produces liquid seaweed manure in the world and enter the world market, wherein South Africa, Britain and New Zealand are the comparatively successful countries of production seaweed fertilizer, they produce the liquid seaweed manure of kiloton every year, though producing the crucial technology of this liquid fertilizer is the technology of clearing up of frond, frond is thoroughly cleared up and can be made the whole strippings of effective constituent and keep active.Clear up with ultrasonic wave at present or the glycolysis method is an optimised process.The development of this product is also being set about from the nineties by China.
4, speak of the problem of tankage fermented liquid again, the tankage of livestock and poultry fish processed goods and the byproduct after the processing of farm products possess multiple functional factor and nutrition, as: 1. active polysaccharide, as food fibre, fungus polysaccharide etc.; 2. functional sweetener is as soybean oligosaccharide, fructose etc.; 3. functional grease is as unsaturated fatty acids, phosphatide, choline etc.; 4. vitamins is as VA, Vc, VB etc.; 5. active skin, the sweet skin of Ru Guguang, immunoglobulin (Ig) etc.; 6. active mattress class is as bifidus bacillus etc.; 7. trace element as selenium, zinc, germanium etc., also has the plant function factors such as saponin(e, polyphenol.Make full use of tankage and all be beneficial in the people in state, according to parties concerned statistics, it is very big that China delivers livestock and poultry quantity every year for sale, 3.5 hundred million of pigs, 1,500 ten thousand of oxen, 100,000,000 of sheep, 3,200,000,000 of fowl, only the animal blood amount just reaches 900,000 tons, if processing and utilization not, source of pollution are very serious, be the considerable wealth of a stroke count amount, both solved the wasting of resources and environmental pollution, saved the environmental improvement expense, for livestock industry provides the fine animal feedstuff additive, be the measure of a multiple-effect benefit again.
5, the present invention adds photosynthetic bacteria at blended material (marine alga fermented liquid and tankage fermented liquid, the trace element of preparation are mixed), biological bacteria such as milk-acid bacteria, yeast can be further with the not material slaking of fully fermenting, and milk-acid bacteria can not only hydrolyzed starch, hydrolyzable phytic acid also, this is more rare in like product.Increase this class probiotics in animal body; there is not the defective of phytase to be of great benefit to remedying monogastric animal; it improves the utilization ratio of phytate phosphorus; reduce the antinutritional factor in the daily ration; directly improve the transformation efficiency of feed; promote the growth of organs such as animal skeleton, also reduced ight soil row phosphorus amount simultaneously, environment protection is of great benefit to.The present invention is meaningful greatly to the excrement phosphorus evil problem that reduces, control makes environment specialists worry.This also is of the present invention one big characteristics and intention.In addition, also have photosynthetic bacteria among the present invention, it can decompose objectionable impuritiess such as the interior ammonia-state nitrogen that produces of livestock and poultry body, nitrous acid, hydrogen sulfide, reduces the stink of ight soil, reduces the pollution of farm animal excrement to environment.Therefore itself still fine thalline high protein simultaneously, and contain abundant methionine(Met) have one's own knack improving on the animal immunizing power.Photosynthetic bacteria also has introduction to the promoter action of plant in many documents, after the report that has uses photosynthetic bacterium fertilizer, can effectively increase the number of blade, thereby increase photosynthesis of plants, so that increase the biomass of plant, after using photosynthetic bacterial fertilizer, the plant number of shoots increases, and leaf is greener than not using the common plant of photosynthetic bacterial fertilizer.
Photosynthetic bacteria of the present invention, milk-acid bacteria, yeast can arrive the microorganism center and buy, and also can oneself cultivate, and the composition and the content of substratum are as follows:
Milk-acid bacteria substratum: yeast extract paste 0.3%～1.0%, soy peptone 0.1%～1.0%, glucose or lactose 0.3%～2.0%, K
2HPO
40.05%～0.5%, Tomato juice 5%～10%, tween 80 0.01%～0.1%, 35～38 ℃ of culture temperature, static cultivation.
Photosynthetic bacteria substratum: NH
4Cl 0.05%～0.2%, K
2HPO
40.01%～0.5%, MgCl
20.01%～0.2%, NaCl0.05%～1.0%, yeast extract paste (powder) 0.005%～0.5%, NaHCO
30.01%～2.0%, ethanol 0.1%～0.5% is used H
3PO
3Transfer PH7.0, photosynthetic bacteria culture condition: 25～32 ℃ of illumination cultivation of anaerobism.
Microzyme culture medium: 10 ° of BX worts, 25～30 ℃ of static cultivation.
More than said yeast comprise solid-state, semi-solid state, liquidly cultivate the concentrating of microbial inoculum and they, dilution, absorption, spraying drying, vacuum-drying agent.
More than said milk-acid bacteria comprise the concentrating of semi-solid state, the liquid microbial inoculum of cultivating and they, dilution, absorption, vacuum-drying agent.
More than said photosynthetic bacteria comprise solid-state, semi-solid state, liquidly cultivate the concentrating of microbial inoculum and they, dilution, absorption, spraying drying, vacuum-drying agent.
Milk-acid bacteria, yeast all are good aid digestion probiotic bacteriums, and still nutritious unicellular high protein of thalline itself, and they are in edible, feeding all widespread usage.
The simple production technical process of biological bacteria of the present invention is as follows:
1.--1L transparent vessel--10L transparent vessel--50L transparent vessel is ripe plants the photosynthetic bacteria seed
2. yeast inclined-plane seed-1000ml triangular flask kind-0.04m
3-0.2m
3Ripe microbial inoculum
3. the straight cup of milk-acid bacteria inclined-plane kind-500ml kind-0.04m
3--0.2m
3Ripe microbial inoculum
The present invention compared with prior art, its outstanding substantive distinguishing features and obvious improvement is:
Aminoacids complex ecoalimental of the present invention agent contains the elite of a large amount of beneficial microbe colonies, marine organisms, be equipped with the tankage fermented liquid, the necessary amino acid of animal and plant growth, protein, various trace elements and ecologic active enzyme etc. are contained in inside, form through modern biotechnology is refining, nontoxic, nuisanceless, pollution-free, the no hormone of product, directly apply to vegeto-animal growth, the growth value-added effect of highly significant is arranged, increase production and efficiently reach 100%; And has a magical effect of winter protection, drought resisting, degeneration-resistant, disease resistance, inhibition pathogenic agent, strengthening immunity.Be that to compile plantation, breed, plant protection function be the high-tech ecological environment-friendly type product of one, once obtained calendar year 2001 " the 13 national invention copper prize ".
Aminoacids complex ecoalimental of the present invention agent (ProductName " ecological garden ") has following seven big functions:
1, the effect of increasing income is good: after the agent of aminoacids complex ecoalimental is applied to all kinds of plants, absorbed very soon by the blade pore, directly enter in the plant materials, increase chlorophyll content in leaf blades, thereby the raising photosynthesis rate is accelerated the formation and the growth of cell are accelerated in the conversion of various nutritive elements and synthetic, make plant strain growth quick, increasing both production and income.After " ecological garden " absorbed in the animal body, can improve the bioavailability of trace element and the utilization ratio of compound body organic substance, absorb easily, the nutrient general equilibrium promotes cell fission, and growth increases weightening finish fast.
2, multifunctionality: aminoacids complex ecoalimental agent prescription uniqueness, both can be widely used in plant husbandry such as melon dish, fruit tree kind, grain class, mushroom class, flowers class, silkworm class, tea, careless class, can be applicable to the breed of land and water animals such as pig, chicken, duck, goose, shrimp, crab again.Plantation and breeding production are organically combined, not only save time but also save trouble.
3, diseases prevention, disease-resistant, immunizing power is strong: after the agent of aminoacids complex ecoalimental is absorbed by organism, not only can promote the metabolism of cell, and strengthen the immunizing power of self very effectively, improve disease-resistant rate, reduce sickness rate, and some diseases are had preventive and therapeutic effect.In addition, " ecological garden " all has the better prevention effect to the various physiological disturbances (as yellows, plant dwarfing and the various illnesss of epidermis) that " nutritional deficiency ", nutrient imbalance cause." ecological garden " not only can improve the appearance character of agricultural-food, also can improve the interior quality of product, thereby improves the commodity rate and the market competitiveness of agricultural-food.
4, winter protection, drought resisting, strong stress resistance: the agent of aminoacids complex ecoalimental can make the plant root prosperity, and the ability that absorbs moisture and fertilizer improves more than 10 times.Under arid, cold adverse environmental factor, plant leaf is breathed pore by physiological regulation, can dwindle and close, and reduces the evaporation of moisture in the plant materials, makes transpiration rate intensity reduction by 20～45%.Synthetic protein, nucleic acid, phosphatide, enzyme and VITAMIN etc. can promote division, the formation of cell in the organism, improve the water-retaining capacity of cell, the discharge of the material that has additional nutrients, thus strengthen winter protection, degeneration-resistant, the physiological function that delays senility.
5, nontoxic, nuisanceless, pollution-free: the agent of aminoacids complex ecoalimental itself does not contain Toxic matter, is applied to agriculture production, and no residual hazard is free from environmental pollution.Micro-and amino acid whose inner complex is a kind of very effective and special biological growth liquid, under the active catalytic of kinases, lyase, can alleviate the accumulation and the infiltration of nitrate and nitrite, reduces agricultural-food and poisons, and reduces and pollutes; Simultaneously, the calcium in the trace element, zine ion can prevent the murder by poisoning of metal ion; Many amino acid whose side chains contain hydroxyl, amino, carboxyl etc. and have chemically reactive gene, can alleviate toxicity of pesticide, meet pesticide residue, and very fast formation mixture dilutes, dissolves residual hazard.
6, reduce cost, increase benefit: owing to the very fast passive plant absorbing of aminoacids complex ecoalimental agent, conversion, activation, growth, and has an effect of disease resistance, the user is when plantation, can reduce by 50% fertilizer amount and reduce poisonous pesticide dosage, also can reach the effect of increasing both production and income, so not only save cost, and really reach the effect of ecological pollution-free, green food.When culturing, the agent of aminoacids complex ecoalimental can be mixed coarse fodder and be fed, thereby reduces the consumption of concentrated feed, reduces cost; And the agent of aminoacids complex ecoalimental is looked soon animal, has shortened the production cycle, has saved human and material resources, has improved economic benefit and social benefit greatly, is the first-elected product that develops eco-agriculture, builds green food.
7, the agent of aminoacids complex ecoalimental is to soil compaction, and especially harden in the arable land of causing because of the life-time service fertilizer and pesticide, and the solid effect of loose reconciliation of highly significant is arranged.The experiment proved that, this product has the seepage force and the intrinsic expansion decomposing force of extra-heavy, it is lasting to render a service, the saturating part of the liquid soil property of can both loosening effectively, improve the physical behavior of soil, strengthen the integration capability and drought-enduring, the performance of keeping humidity of ridging, improve the soil ecology balance, make the plant of growing period remove intertillage from.Under hydrogen, oxygen, charcoal and photosynthesis, separate the organic and inorganic composition in the solid soil property rapidly, excite the vigor of root system cell, improve receptivity and the fertilizer efficiency utilization ratio of root system various nutritive substances, and can solve the physiological disease that causes because of nutritional deficiency effectively, improve immunizing power.Reached can loosen the soil, no-tillage, can make the good result that plant speed is long, improve quality and increasing both production and income again.This product is nontoxic, harmless, noresidue, pollution-free, and this is the first-selected product that develops eco-agriculture, improves the soil, builds organic good farmland, production pollution-free green food the new millennium.
8, tankage is through handling with papoid or subtilisin, stomach en-or mixed enzyme, and virus has been destroyed most of by enzyme, so be safe as the fodder additives of animal.
Embodiment
Embodiments of the invention
Marine alga fermented liquid production example 1: plants such as marine red alga or brown alga, green alga suitably are broken into fragment below 2 centimetres, press the per kilogram marine alga and add the 8-20 kg of water, add 1% cellulase and 2% milk-acid bacteria and 2% zymic mixture again by the marine alga total amount, make its condition at 25 ℃, fermentation obtained the marine alga fermented liquid after 2 days in jar fermenter.
Marine alga fermented liquid production example 2: plants such as marine green algae or brown alga, green alga suitably are broken into fragment below 2 centimetres, press the per kilogram marine alga and add the 8-20 kg of water, add 1% cellulase and 1.5% milk-acid bacteria and 2.5% zymic mixture again by the marine alga total amount, make its condition at 35 ℃, fermentation obtained the marine alga fermented liquid after 2 days in jar fermenter.
Tankage fermented liquid production example 1: after tankage such as the bone that abandons in pig, ox, sheep, chicken, duck, goose, the fish course of processing, blood, liver, intestines, tire, tripe, fish scale, the fish gill are smashed, add the 8-20 kg of water by animal tankage per kilogram, the papoid or the subtilisin that add 1-5% again by animal tankage total amount, or stomach en-, make its condition at 25 ℃, in jar fermenter, ferment, obtain the tankage fermented liquid after 1-10 days.
Tankage fermented liquid production example 2: after tankage such as the bone that abandons in pig, ox, sheep, chicken, duck, goose, the fish course of processing, blood, liver, intestines, tire, tripe, fish scale, the fish gill are smashed, add the 8-20 kg of water by animal tankage per kilogram, the papoid or the subtilisin that add 1-5% again by animal tankage total amount, or stomach en-, make its condition at 35 ℃, in jar fermenter, ferment, obtain the tankage fermented liquid after 1-10 days.
Biological bacteria is cultivated embodiment: photosynthetic bacteria is put into NH
4Cl 0.05%～0.2%, K
2HPO
40.01%～0.5%, MgCl
20.01%～0.2%, NaCl 0.05%～1.0%, yeast extract paste or yeast powder 0.005%～0.5%, NaHCO
30.01%～2.0%, ethanol 0.1%～0.5% is used H
3PO
3Transfer in the substratum of PH7.0 culture condition: 25～32 ℃ of illumination of anaerobism, obtain photosynthetic bacteria after the cultivation and enlarge bacterium, again by bacterium 1-15%, photosynthetic bacteria 1-15%, the ratio that all the other are mixes, and obtains.
Milk-acid bacteria is cultivated embodiment: milk-acid bacteria is put into the milk-acid bacteria substratum: yeast extract paste 0.3%～1.0%, soy peptone 0.1%～1.0%, glucose or lactose 0.3%～2.0%, K
2HPO
40.05%～0.5%, Tomato juice 5%～10%, tween 80 0.01%～0.1%, 35～38 ℃ of culture temperature, static cultivation obtains.
Yeast is cultivated embodiment: yeast is put into 10 ° of BX worts, and 25～30 ℃ of static cultivation obtain.
Aminoacids complex ecoalimental agent production example:
The production example 1 of aminoacids complex ecoalimental agent: prepare marine alga fermented liquid and tankage fermented liquid respectively by the foregoing description earlier, get 20 kilograms of marine alga fermented liquids, 45 kilograms of tankage fermented liquids, add 0.2 kilogram of micro-Ferrous Gluconate, 0.05 kilogram in zinc sulfate, 0.05 kilogram in copper sulfate, 0.2 kilogram of grape calcium gluconate, 0.001 kilogram of sodium selenate adds water again and reaches 100 kilograms, then it is mixed at normal temperatures, add photosynthetic bacteria again, milk-acid bacteria, yeast, every kind of bacterium is 0.5 kilogram, reaction is 1-2 days in container, filter, obtain being fit to the product of raise poultry domestic animal, bottling can be dispatched from the factory.
The production example 2 of aminoacids complex ecoalimental agent: prepare marine alga fermented liquid and tankage fermented liquid respectively by the foregoing description earlier, get 25 kilograms of marine alga fermented liquids, 35 kilograms of tankage fermented liquids, add 0.1 kilogram in micro-ferrous sulfate, 0.1 kilogram in zinc sulfate, 0.05 kilogram in copper sulfate, 1 kilogram of potassium primary phosphate, 0.5 kilogram in urea adds water again and reaches 100 kilograms, then it is mixed at normal temperatures, add photosynthetic bacteria again, milk-acid bacteria, yeast, every kind of bacterium is 0.5 kilogram, reaction is 1-2 days in container, refilter, obtain plant promoting agent, bottling can be dispatched from the factory.
The production example 3 of aminoacids complex ecoalimental agent: prepare marine alga fermented liquid and tankage fermented liquid respectively by the foregoing description earlier, get 20 kilograms of marine alga fermented liquids, 40 kilograms of tankage fermented liquids, add 0.15 kilogram of micro-Ferrous Gluconate, 0.1 kilogram in zinc sulfate, 0.05 kilogram in copper sulfate, 0.05 kilogram of manganous sulfate, 0.5 kilogram of grape calcium gluconate adds water again and reaches 100 kilograms, then it is mixed at normal temperatures, add photosynthetic bacteria again, milk-acid bacteria, yeast, every kind of bacterium is 0.5 kilogram, reaction is 1-2 days in container, refilter, obtain being fit to the product of raise poultry domestic animal, bottling can be dispatched from the factory.
The production example 4 of aminoacids complex ecoalimental agent: prepare marine alga fermented liquid and tankage fermented liquid respectively by the foregoing description earlier, get 30 kilograms of marine alga fermented liquids, 30 kilograms of tankage fermented liquids add 0.1 kilogram in micro-ferrous sulfate, 0.1 kilogram in zinc sulfate, 0.05 kilogram in copper sulfate, 1 kilogram of potassium primary phosphate, 0.5 kilogram in urea adds water again and reaches 100 kilograms, add water again and reach 100 kilograms, then it is mixed at normal temperatures, add photosynthetic bacteria, milk-acid bacteria again, yeast, every kind of bacterium is 0.5 kilogram, reaction is 1-2 days in container, refilters, and obtains plant promoting agent, bottling can be dispatched from the factory.
The using method of aminoacids complex ecoalimental agent: be applied to animal cultivation, every pig (per 20 chicken and duck) 10-30 milliliter every day.Be applied to plantation, per 100 milliliters of nutrition agents add water 10-25 kilogram, can spray 1-1.5 mu grain and vegetables.
Aminoacids complex ecoalimental of the present invention agent is applied to the experimental data of animal cultivation and plantation:
(1) nanmu mountain in Dongxing City, Guangxi is supported the shrimp field and is used the trial report that ecological garden biological growth liquid is raised prawn
One, test responsible official: the professor of the Chinese Academy of Sciences, senior engineer officer: Wang Nanqi
Two, test conditions:
1, test site: shrimp field, nanmu mountain, Guangxi province Dongxing City
2, test period: on May 8th, 2003 to August 5 (totally 87 days)
3, test area: 20 mu (wherein the test site is 10 mu, 10 mu of check plots, identical water quality, water temperature, weather, raising condition.)
4, shrimp pool situation: hang down and enclose Chi Tutang, take out seawater and irritate foster.
5, shrimp pool condition: meet the basic demand of raising prawn.
6, shrimp species: Penaeus vannamei.
7, shrimp seedling supply volume: average every mu 45000 tail.
8, feed: Penaeus vannamei feed.
9, oxygen system: electronic aerator.
10, water quality: good, pH value regulates and control 8～8.3.
Three, test method:
Enclosing with 20 mu low that Chi Tutang divides equally is two, is respectively 10 mu of test sites, 10 mu of check plots.Put the shrimp seedling on May 8th, 2003 simultaneously in a suitable place to breed, put Penaeus vannamei seedling 450,000 tails in a suitable place to breed for per 10 mu.
Test group method for breeding routinely, add the special-purpose liquid of " ecological garden " foster shrimp that ecological garden, Nanning city Bioisystech Co., Ltd produces, the concrete ratio of using by specification to divide seedling phase, mid-term, finishing period is added, and admixes the shrimp feed and raises by normal appetite.Control group method for breeding routinely adds shrimp medicine and microbiotic.After 87 days contrasts are raised, in the pool on August 5th, 2003.Comparing result is reported as follows:
Four, comparative test result consolidated report table
| Feeding time (my god) | Feed total amount (kg) | Ultimate production (kg) | Average per kilogram shrimp materials (kg) | Average yield per mu kg | Average every mu of volume increase (k g) | Average stimulation ratio (%) | Average per kilogram shrimp mantissa (tail/kg) | Medicine or additive investment total charge (unit) | Average per kilogram shrimp medicine incidentals (unit) | Sales price (unit/kg) | Sales price (unit) | Increase income (unit) | Increase income rate (%) | Comprehensive per kilogram shrimp cost (unit) | (%) comprehensively increases benefit | |
| The check plot | 87 | ?6000 | ?5250 | ?1.14 | 525 | - - | ??- ??- | 76～ 80 | 10000 | 1.9 | 30 | 157500 | - | - - | 8.74 | - |
| The test site | 87 | ?6500 | ?6930 | ?0.938 | 639 | 168 | ??32 | 60～ 64 | 5500 | 0.8 | 30 | 207900 | 50400 | 32 | 6.428 | 46.5 |
Illustrate: 1, the feed price average out to is 6 yuan/kilogram; 2, composite item cost is only counted feed and medicine incidentals, and the artificial and electricity charge are disregarded.
Five, test effect analysis 1, from test-results, the test site is used " ecological garden " and has been reached very significant effect, increase fast, the surviving rate height, diseases prevention, disease-resistant, immunizing power is strong, reduces cost and risk, comprehensive augmentation ratio on average increases production 32% up to 46%.
2, the test site reached 60～64 tail/kilograms in 87 days, and check plot 87 talentes, 76～80 tail/kilograms, raising need just can reach 60 tail/kilograms about 115 days routinely.So can do sth. in advance listing up to standard in 28 days with " ecological garden " raising, and meat is good, sparkling and crystal-clear bright, body is evenly strong, the selling price height.
3, July when shrimp pond density is big, be subjected to this year 6, No. 7 typhoons and late July continuous high temperature weather influence, test group is safe and sound, and the dead volume phenomenon of group shrimp appears in control group, for reducing the loss, under the also below standard situation of prawn, play the pool in advance.So proof, " ecological garden " not only can increase fast, and the function of very strong opposing natural disaster and the various diseases of defence is arranged, and reduced the risk at foster shrimp family significantly, guarantees high yield and bumper harvest.
4, conclusion: " ecological garden---support the special-purpose liquid of shrimp " make an addition in the shrimp feed and raise prawn, it is very remarkable that prawn increases gaining effect, and very strong immunologic function is arranged simultaneously, is that current scientific and technological content is higher, the good product that is worth promoting.
(2) Plantula Brassicae chinensis is used " No. 1, ecological garden " and sprays the blade face trial report
Agricultural extension testing station, Qinzhou City is subjected to the trust of ecological garden, Nanning City Bioisystech Co., Ltd, sprays effect for " No. 1, the ecological garden " of verifying its development and production contains aminoacid foliar fertilizer on Plantula Brassicae chinensis, has carried out the sub-district manure trial in the calendar year 2001 6-8 month; Now test-results is reported as follows:
1 materials and methods
1.1 test site and edaphic condition
Carry out in the test city of admiring in Qinzhou City, is damp Shatian for examination soil, middle fertility.
1.2 for examination fertilizer
For examination fertilizer for " No. 1, ecological garden " contain amino acid foliage fertilizer (aqua), Bioisystech Co., Ltd provides after testing by ecological garden, Nanning City, hydrogen base acid total content is 115.8g/l, micro-total content 34.3g/L.
1.3 for studying thing
For studying thing is Plantula Brassicae chinensis
1.4 test design
Three processing are established in test, three repetitions, and district's group is arranged at random, and the sub-district area is that 20 square metres of processing are as follows:
Handle 1: spray " No. 1, ecological garden " contains amino acid foliage fertilizer.Foliage-spray is three times in vegetative period.Converting water 50kg with this foliage fertilizer 20ml carries out foliage-spray at every turn to amount to per 666.7 square metres.
Handle 2: the spray clear water.It is identical with processing 1 to spray time, number of times and consumption.
Handle 3: blank (not spraying anything).
The soil application of test is undertaken by following requirement: organic fertilizer not, only to fertilize.Per 666.7 square metres of fertilizer amounts are amounted to N-10kg, P
2O
5-1.5kg, K
2O-5.0kg, application process---the purity nitrogen of whole phosphate fertilizer, potash fertilizer and 1.0kg is made base manure, executes down when whole ground, should cover thin soil after the fertilising; 9.0kg purity nitrogen topdress, divide and carry out for four times, executed 1.0kg, 2.0kg, 3.0kg and 3.0kg behind the survival after transplant in 3 days, 10 days, 15 days and 20 days respectively, adopt and convert water and drench applying method and carry out.
Field management measure requires to carry out routinely.
1.5 process of the test and observation
July 9 whole ground, play furrow, July 10, subregion was quantitatively sowed (broadcasting sowing), (specification is 15cm * 15cm) for thinning during two true leaves, final singling.Soil application is undertaken by the scheme requirement, and foliage fertilizer sprays three times in August 1, August 8, August 15 respectively.August 31, results adopted main plot results weighing.Spray the leaf look and the growth potential of observing plant in 5 days behind the foliage fertilizer at every turn.Observe for the first time, leaf look, growth potential difference between each is handled are not obvious; Second and third time observation handles 1 and handle 2,3 ratios, and the leaf look greener, handle 2 with handle 3 between the leaf aberration different not obvious, observe for the third time that to handle 1 blade bigger, plant height is higher, growth potential is 1 more prosperous, more neat to handle, and handles 2, processing 3 is poor slightly.
2 results and analysis
2.1 different treatment is to the influence of Plantula Brassicae chinensis biological character
The preceding every sub-district of results gets 20 strains mensuration plant height at random continuously and strain weighs, and pats mean everywhere and sees Table 1.
Table 1 different treatment biology shape table
| Handle | Plant height (cm) | Individual plant is heavy |
| Handle 1 | ????22.9 | ????55.0 |
| Handle 2 | ????22.2 | ????50.0 |
| Handle 3 | ????22.1 | ????42.0 |
From table 1, processing 1 is compared with processing 2,3, and plant height, individual plant weight average have increase, and plant height on average increases 0.7cm and 0.8cm respectively, heavy 5.0g and the 13.0g of on average increasing respectively of individual plant.Illustrate the Plantula Brassicae chinensis foliage fertilizer spray " No. 1, ecological garden " contain amino acid foliage fertilizer to increase plant height, individual plant heavily has certain effect.
2.2 different treatment is to the influence of the yield of pakchoi and the output value
2.2.1 output
Different treatment Plantula Brassicae chinensis actual output sees Table 2
Table 2 different treatment actual output table kg/666.7m
2, kg, %
| Handle | Repeat | On average | With processing 3 ratios | With processing 2 ratios | ||||
| ?1 | ?2 | ?3 | Volume increase | Increase | Volume increase | Increase | ||
| Handle 1 | ?1033.4 | ?1066.7 | ?1050.1 | ?1050.1 | ?113.4 | ?12.1 | 84.5 | 8.7 |
| Handle 2 | ?946.7 | ?996.7 | ?953.4 | ?965.6 | ?28.9 | ?3.1 | ||
| Handle 3 | ?903.4 | ?966.7 | ?940.0 | ?936.7 | ||||
2.2.2 variance analysis
Test output is carried out variance analysis, the results are shown in Table 3.
Table 3 variance analysis
| Become because of | Degree of freedom | Sum of squares | All square | The F value | F 0.05 | ?F 0.01 |
| Between processing | 2 | ?20812.0 | ?10405.98 | ?15.41 ** | 5.14 | ?10.92 |
| In handling (error) | 6 | ?4052.3 | ?675.38 | |||
| Total variation | 8 | ?24864.3 |
F value (15.41) is greater than F as can be seen from Table 3
0.01(10.92), illustrate that the processing differences is extremely remarkable.
2.2.3 multiple comparisons
Adopt duncan's new multiple range method to carry out multiple comparisons to yield result, the results are shown in Table 4.
The table 4 different treatment significance of difference (SSR check)
| Handle | Mean yield (kg/666.7m 2) | The significance of difference | |
| ?α＝0.05 | ?α＝0.01 | ||
| ?1 ?2 ?3 | ?1050.1 ?965.6 ?936.7 | ?a ?b ?b | ?A ?B ?B |
LSR during P=2
0.05=51.91, LSR
0.01=78.62; LSR during P=3
0.05=53.71, LSR
0.01=82.67
As can be seen from Table 4, processing 1 and processing 2,3 ratios, significant difference, processing 2 and processing 3 ratios, difference is not remarkable.
2.2.4 the output and the output value are relatively in different the producing
The different treatment output and the output value relatively see Table 5
Table 5 different treatment output and output value comparison sheet kg/666.7m
2, unit/666.7m
2
| Handle relatively | Volume increase | Increase income | Additional cost | Pure increasing income | |
| Handle 1 than handling 2 | ?84.5 | ?84.5 | ?12.0 | ?72.5 | |
| Handle 1 than handling 3 | ?113.4 | ?113.4 | ?12.0 | ?15.0 | ?86.4 |
| Handle 2 than handling 3 | ?28.9 | ?28.9 | ?15.0 | ?13.9 | |
3 conclusions
From this test-results, Plantula Brassicae chinensis sprays the development and production of ecological garden, Nanning City Bioisystech Co., Ltd " No. 1, ecological garden " contain amino acid foliage fertilizer and spray clear water ratio: (1) stimulation ratio is 8.7%; (2) one-factor difference reaches utmost point conspicuous level through biometrical verification; (3) average every 666.7m
2Net return is 72.5 yuan.
" ecological garden " edible mushrooms tailored version biological growth liquid foliage fertilizer is used on flat mushroom
Illustrate:
1, subjects: second batch of flat mushroom (No. 138), test 600 bags for every group.
2, test time of origin: end beginning on October 23rd, 2002 on November 5th, 2002.
3, test site: Tunxi District poulty house, Huangshan.
4, test householder: fourth wealth year.
5, test method: 1. foliar spray once next day of testing 1 group; 2. test 2 groups every foliar spray on the 2nd 1 time.3. contrast without foliage fertilizer Routine Management.4. foliage fertilizer working concentration: the 5ml foliage fertilizer adds cleaning drinking-water and stirs evenly 4 foliage spray that afternoon for 10 kilograms.
6, other conditions of test group and control group are the same, from on-test to finish amounting to 14 days.
7,1 group of output of test is 180.5 jin, a traditional unit of weight; Test 167.6 jin, a traditional unit of weight of 2 groups of output; 139.4 jin, a traditional unit of weight of control group output.
8,2 groups of fecund of 1 group of ratio test of test are 12.9 jin, a traditional unit of weight, volume increase 7.7%; Test 1 group than 41.1 jin, a traditional unit of weight of control group fecund, volume increase 29.5%; Test 2 groups than 28.2 jin, a traditional unit of weight of control group fecund, volume increase 20.2%.
9, sampling is at random, does not add person in charge's behavior of people.
10, " ecological garden " foliage fertilizer is by 0.2 yuan/1ml, and input-output ratio is 1: 4.
11, this test is formulated testing program by Huangshan science and technology testing station, is responsible for enforcement by comrade Ge Gusheng.Not through " biometrics processing ".
Product of the present invention has obtained the interim registration card of People's Republic of China's fertilizer through the authorization of fertilizer registration evaluation committee of The Ministry of Agriculture of the People's Republic of China, MOA in March, 2004.
Claims (6)
1, a kind of aminoacids complex ecoalimental agent is characterized in that it comprises marine alga fermented liquid and tankage fermented liquid, trace element, biological bacteria, and its composition and mass content are as follows:
Marine alga fermented liquid 20-30%
Tankage fermented liquid 20-45%
Biological bacteria: 1-5%
Trace element 0.5-1%
All the other are water.
2, aminoacids complex ecoalimental according to claim 1 agent is characterized in that: the marine alga fermented liquid liquid that to be plants such as the red algae that will grow in the ocean, brown alga, green alga obtain with cellulase and milk-acid bacteria and zymic fermented mixture.
3, aminoacids complex ecoalimental according to claim 1 agent, it is characterized in that: the tankage fermented liquid is meant that tankage such as the bone that will abandon in pig, ox, sheep, chicken, duck, goose, the fish course of processing, blood, liver, intestines, tire, tripe, fish scale, the fish gill smash the back with papoid or subtilisin, or the liquid that obtains of stomach en-fermentation.
4, aminoacids complex ecoalimental according to claim 1 agent is characterized in that: trace element comprises mineral compound, the organic compound of iron, copper, zinc, calcium, selenium, manganese.
5, aminoacids complex ecoalimental according to claim 1 agent is characterized in that: biological bacteria is a photosynthetic bacteria, milk-acid bacteria, yeast, and every kind of bacterium of its weight content is 1: 1: 1.
6, a kind of aminoacids complex ecoalimental as claimed in claim 1 agent, it is characterized in that: the production method of aminoacids complex ecoalimental agent is to prepare earlier marine alga fermented liquid and tankage fermented liquid respectively, add trace element earlier, then it is mixed at normal temperatures, add biological bacteria again, reaction is 1-2 days in container, refilters, packing obtains product.
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