CN107624503A - It is a kind of to produce fruiting bodies of cordyceps militaris and fish feed additive method simultaneously - Google Patents
It is a kind of to produce fruiting bodies of cordyceps militaris and fish feed additive method simultaneously Download PDFInfo
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- CN107624503A CN107624503A CN201711066845.0A CN201711066845A CN107624503A CN 107624503 A CN107624503 A CN 107624503A CN 201711066845 A CN201711066845 A CN 201711066845A CN 107624503 A CN107624503 A CN 107624503A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses one kind while produces fruiting bodies of cordyceps militaris and fish feed additive method.Production fruiting bodies of cordyceps militaris culture medium prescription is mixed by A liquid and B solids：A liquid components are as follows：1000 ml water, add sucrose 10.0~15.0 g, KH2PO4 0.3~0.5 g, MgSO40.3~0.5 g, vitamin B compound 4, vitamin C 1.B solids composition is as follows：Wheat berry 50%, the vegetable seeds dregs of rice 15%, peanut meal 24%, dry dried silkworm chrysalis meal 1%, rice bran 10%；The ratio of A liquid and B solid powders is 2~3：1；The culture medium is sealed, sterilizes, cooling；Then Cordyceps militaris spawn is inoculated with, inoculum density is 10~20 bacterium ball/mL, is 1 by strain and culture volume ratio：50 are inoculated with, and during culture, are first carried out light culture, are carried out optical culture again；Obtain fructification and bacteria residue.Bacteria residue reaches 27.43% containing Thick many candies.Bacteria residue is crushed to 80 mesh granularities, is advantageous to fish body and digests and assimilates after naturally dry or drying, and smell Fen Uu, can be used as fish feed additive, addition 10~15%.
Description
Technical field
The present invention relates to the culture technique of Cordyceps militaris and fish feed Additive Production field, and in particular to a kind of Cordyceps militaris
Application of the bacteria residue in fish feed and feed addictive after culture medium prescription, and the production fruiting bodies of cordyceps militaris.
Background technology
Cordyceps militaris（Cordyceps militaris）, also known as Cordceps militaris, it is under the jurisdiction of Ascomycotina, the ergot of mycota
Cordycepps, Cordyceps.Fruiting bodies of cordyceps militaris has similar medicinal and nourishing work efficiency to cordyceps sinensis, and the cordycepin in Cordyceps militaris contains
Amount is a kind of rare medicinal fungi higher than tens times of traditional cordyceps sinensis.Cordyceps militaris contains abundant protein, amino acid
And the trace element of more than 30 kinds of needed by human body, and containing distinctive cordycepin, cordycepic acid be more etc., that physiologically active ingredient has is anti-
Function of tumor.Modern medicine and pharmacology research has shown that, the nutriment in Cordyceps militaris have nourishing the lung and the kidney, hemostasis and phlegm, extension tracheae,
Calmness, antibacterial, hypotensive, anti-oxidant, protection liver, strengthen immunity and other effects.The Ministry of Public Health has been approved by Cordyceps militaris within 2009
For new resource food.In terms of Cordyceps militaris synthetic medium, there are many patents, such as application number：A kind of 201310462899.4 pupa
Culture medium of Cordyceps militaris and its application, the culture medium used are rice and pupa worm powder；And for example, application number：It is 201510397546.X a kind of
Culture medium of Cordyceps militaris and preparation method thereof is cultivated, culture medium is cereal and dry silkworm chrysalis；It there are no and use rapeseed meal and peanut meal
Cordyceps militaris is produced for auxiliary material.
It is new technology developed in recent years with Tissue Culture of Cordyceps militaris such as rice and dried silkworm chrysalis meals, after fructification harvesting,
Cover with mycelial culture medium residue and be but treated as waste material discarding, cause the serious wasting of resources and environmental pollution.Cordyceps sinensis bacteria residue
（Edible Fungi Residue, EFR) it is remaining after fruit body of edible fungi harvest during Chinese caterpillar domestic fungus is cultivated
Cultivate based draff.The edible medicinal bacteria residue yield in China is also surprising, and bacteria residue is very difficult to degraded in natural environment, easily
Mould growth, insect, welding, and burn and then produce exhaust emission environment.So do the biology money for not only wasting preciousness
Source, and significant environmental problem is caused, so rational exploitation and utilization, realizes the cycling and reutilization of bacteria residue with regard to necessary.
This patent is developed aiming at cordyceps sinensis bacteria residue as feed resource.
After fructification harvesting, every bottle of discarded culture medium residue dry weight is about 40g, and cordycepin contained therein is about
For 0.5%, total sugar content be about 21.93%, protein content is about 10%, Cordyceps sinensis polysaccharide content is about 12.86%, is additionally contained
The active ingredients such as alkaloid, terpenoid, sterol, glycoside, phenols, enzyme, vitamin, SOD, still there is very big exploitation value
Value.Cordycepin (Cordycepin) has antibacterial, antitumor, antiviral, antagonism oxygen radical and other effects, has higher medicinal
Value and extensive pharmacological action, are the heavier a kind of physiological activators regarded of pharmacy circle.If apply it to feed formula
In, the multiple nutritional components in culture medium residue can be made full use of, and feed cost can be reduced, reach the effect to turn waste into wealth
Fruit.
Bacteria residue is compared to for making the raw material of original culture medium of edible fungus, and its nutritional ingredient changes, nutriture value
Value significantly improves, and crude fibre is degraded, and content is greatly lowered, accordingly, the relative scale lifting of protein, and therefore, this bacterium
Slag is also referred to as mushroom bran albumen.Bacteria residue not only effective nutritional ingredient increase after edible mushroom converts, if being used for feed industry, because
It then greatly improves digestive utilization ratio to coarse-fibred degraded, adds its palatability.
As the improvement of people's living standards, people also improve therewith to the demand and quality requirements of aquatic products.Feed
It is a key factor for influenceing healthy fish growth and aquatic products quality, on the one hand, present most of prawn feeds are ground
Hair primarily focuses on the speed of production and fish crop of prawn, and ignores the raising to its quality.On the other hand, conventional feed formula
The proportions such as middle corn, wheat, dregs of beans are higher, with increasingly soaring, the feed cost more and more higher of provision price, serious shadow
The economic benefit of culture fishery is rung.
Fish, especially prawn have very high nutritive value, and it contains multiple proteins, and meat is soft, is easy to digest,
And rich in abundant mineral matter (calcium, phosphorus and iron), it is loved by consumers.China is the second largest producing country of world's shrimp products and
Four large outlet states, and prawn is one of main shrimp products, the minor fluctuations of prawn yield and quality all can be to aquatic products trade
Produce considerable influence.Prawn market price is higher, but it is susceptible to suffer from prawn disease, and cultivation risk is also larger, if prawn suffers from breeding process
Disease or even death, the quality and quantity of prawn sale can be substantially reduced.
Fish feed quality is to influence the key factor of aquatic ecosystem, fish resistance against diseases and quality.With right
Exemplified by shrimp, prawn feed cost accounts for the 30%~40% of prawn culturing cost, is the maximum items of cost expenditure in cultured prawn
(Duan Yuanyuan, Chinese shrimp products international trade structure and competitiveness research [D] Qingdao：Chinese Marine University, 2009), it is seen then that
Prawn feed turns into one of an important factor for determining prawn culturing economic well-being of workers and staff height.In prawn mixed feed, addition is more
The applications such as sugar, immunopotentiator are more universal, have in terms of Cordyceps sinensis fungus produces feed addictive, existing application number：
200710022923.7 aweto activated reinforced immunological feed additive and its production method.The patent is to utilize Cordyceps militaris and winter
Worm summer grass strain carries out fermented maize, dregs of beans etc., and animal feed additive is used as after fermentation.And for example number of patent application：
97107712.6, Chinese caterpillar fungus feeds additive and its production method, the patent are meat using starchy material production
Animal feed additive.Above-mentioned patent do not referred to wheat berry and peanut meal to produce fruiting bodies of cordyceps militaris, and by its bacteria residue
Apply in fish feed.Therefore, through retrieval, have no using wheat and peanut meal are culture medium to produce Cordyceps militaris, and have no
It is used as fish feed additive using the bacteria residue of Cordyceps militaris, more has no that the correlation that this feed addictive is applied in prawn is special
Profit and document report.
The content of the invention
It is an object of the invention to provide a kind of culture medium prescription for producing Cordyceps militaris and its with Chinese caterpillar fungus culture medium bacteria residue
Feed addictive for raw material and preparation method thereof, can solve the problems such as high existing prawn feed cost and prawn disease resistance difference,
Mitigate the financial burden of fisherman.
The formula for producing Chinese caterpillar fungus culture medium is mixed by the component of A liquid and B solids etc. two：The component of A liquid is as follows：
In 1000 ml running water, sucrose 10.0-15.0 g, KH are added2PO40.3 ~ 0.5 g, MgSO40.3 ~ 0.5 g, it is compound
Vitamin B 4, vitamin C 1；The composition of B solid portions is as follows：Wheat berry 50%, the vegetable seeds dregs of rice 15%, peanut meal 24%, do
Dried silkworm chrysalis meal 1%, rice bran 10%；The liquid-solid ratio of A liquid and B solids is 2 ~ 3：1；And be sufficiently mixed uniformly, load blake bottle, seal,
Sterilizing, cooling.Wherein sterilising conditions are：121 DEG C, 30~45min of 1.1Mpa sterilizings.Chinese caterpillar fungus culture medium provided by the present invention
Available for culture Cordyceps militaris (Fig. 1).
Using the method for above-mentioned Chinese caterpillar fungus culture medium culture Cordyceps militaris, including the step such as be inoculated with, cultivate and harvest drying.Tool
Body is as follows：First it is inoculated with.Cordyceps militaris spawn is inoculated with above-mentioned Chinese caterpillar fungus culture medium, the Cordyceps militaris spawn isCordyceps militaris(L.) Link., numbering are 3.4655, from China General Microbiological culture presevation administrative center (China
General Microbiological Culture Collection Center, CGMCC).Inoculation bacterium is liquid spawn, institute
The density for stating Cordyceps militaris spawn is 10~20 bacterium ball/ml, and the volume ratio of the Chinese caterpillar fungus culture medium and Cordyceps militaris spawn is 50:
1；Cultivated again.The lucifuge culture 5~6 days under the conditions of 25 DEG C of temperature, humidity 80~87%, it is full described to cordyceps filament length
Chinese caterpillar fungus culture medium surface；6th~25 day illumination cultivation used：Fluorescent lamp illumination on 12 hours daytimes, it is 22~25 DEG C of temperature, wet
Degree 80~85%, intensity of illumination are controlled in 150~200 Lx；12 hours black outs at night, 20~23 DEG C of temperature, humidity 80~
83%；Ventilation 2 times daily, once in the morning and once at night, 30 minutes every time；26th~45 day illumination cultivation used：12 hours daytimes are natural
Light adds fluorescent lamp illumination, and 22~25 DEG C of temperature, humidity 80%, total intensity of illumination control is in 1100 Lx；12 hours black outs at night,
20 DEG C of temperature, humidity 75~85%；Ventilation 2 times daily, once in the morning and once at night, 30 minutes every time；Culture light source used is scattered
Light is penetrated, carrying out rolling bottle to blake bottle in incubation makes uniform illumination；Yield ratio uses prior art culture medium and prior art
The fruiting bodies of cordyceps militaris yield increase by 20% that cultural method culture obtains；In per 1g fruiting bodies of cordyceps militaris adenosine content be 15~
20mg, cordycepin content are 7.5~8.0 mg, polyoses content is 25~3 mg, hence it is evident that are trained higher than using art methods
Support obtained Cordyceps militaris.Finally harvested and dried：By fruiting bodies of cordyceps militaris ripe in the blake bottle after step culture
Take out, shine upon or hot air circulation is dried；The Cordyceps militaris bacteria residue of harvest is placed in the stainless steel disc of 80 mesh, uniformly spread out,
40~45 DEG C of hot air circulation drying, its moisture are controlled below 10%.
The present invention adds a little dry dried silkworm chrysalis meal, vitamin etc. as primary raw material, rice bran using wheat berry, peanut meal for auxiliary material, again
Chinese caterpillar fungus culture medium is prepared as nutritional ingredient, and finally determines that culture medium of the present invention is optimal by gradient experiment and matches somebody with somebody
Side, the training method then to be spread cultivation using special three-level：Light culture and then the different illumination cultivation mode of different time sections, then
Plus light source using light is scattered, regularly blake bottle or Culture basin are carried out rolling bottle or turn basin in incubation to make uniform illumination, i.e.,
By determining optimal Chinese caterpillar fungus culture medium formula, on the whole optimization culture scheme, obtained in strict accordance with this method culture
Fruiting bodies of cordyceps militaris is found by detection：Yield ratio is obtained using prior art culture medium and prior art cultural method culture
Fruiting bodies of cordyceps militaris yield increase by 20%；In per 1g fruiting bodies of cordyceps militaris adenosine content be 15~20mg, cordycepin content be
7.5~8.0 mg, polyoses content are 25~3 mg, hence it is evident that higher than the Cordyceps militaris for cultivating to obtain using art methods.
Application of the Cordyceps militaris bacteria residue that the above method of the present invention obtains in fish feed.
The fish feed additive of the present invention is a kind of ecological, environmental protective green bio additive.Utilize the ecological ring of the present invention
Protecting green bio additive is remarkably reinforced prawn resistance against diseases, shrimp seedling and improves 7~9 into life rate in the growth course of shrimp
%, positive effect.The speed of growth of prawn is remarkably reinforced, and in the contrast cultivation of 56 days, is not increasing feedstuff feeding amount, in phase
In the case of forage volume, the prawn of Cordyceps militaris bacteria residue is added, rate of body weight gain improves 4.5~6.0 % (Fig. 2).
The effect that the present invention is furture elucidated is come with contrast test below.
First, experiment condition and contrast experiment's packet.
Experiment seawater is underground sea water, is aerated by precipitation, is persistently inflated in pond, hydrostatic cultivation.The ammonia of experimental water
Nitrogen, nitrite, hydrogen sulfide, pH, dissolved oxygen equivalence are in normal range (NR).27 DEG C of water temperature, salinity 28/1000ths.
Field experiment is industrial aquaculture greenhouse, and each cement pit is（1.5×1.5×1.5）, 3 cubic metres, it is divided into two groups
Every group of 4 cement pits.In 8 cement pits, each cement pit puts the tail of shrimp seedling 200 of 4 grams of average weight in a suitable place to breed.Control group is fed
Common prawn feed；Experimental group feeds the prawn feed for adding this Cordyceps militaris bacteria residue 10%~15%.Feed twice daily.
2nd, tracking measurement and experimental result.
1. the calculating of survival rate：Record the The dead quantity in two groups of parallel laboratory test ponds respectively daily, calculating is drawn after terminating
Average number.
2. measure the body weight of prawn：Metering in every 14 days once, in two groups of parallel laboratory test ponds, does not have individual fishpond to randomly select 5
Tail（Every group of 20 tails）, the body weight of fish is measured with electronic weighbridge, finally sums and averages.
3. the tracking measurement time is 56 days.
4. experimental result is listed below with following forms.
The comparison of 1 two groups of parallel laboratory test contrast survival rates of table.
| Average viability（%） | Remarks | |
| Control group | 86 | Average value |
| Experimental group | 95 | Average value |
The comparison of 2 two groups of parallel laboratory test contrast body weight of table（Unit： g ）.
| 14 | 28 | 42 | 56 | Remarks | |
| Control group | 4.8 | 7.3 | 10.2 | 15.2 | Average value |
| Experimental group | 5.6 | 8.7 | 13.7 | 18.6 | Average value |
3rd, experiment conclusion.
By strict conscientious experiment, environment-friendly and green bio-additive of the invention in the application of prawn culturing technology,
Improve survival rate, the speed of growth etc. significant effect.It has been observed that the prawn body colour of experimental group is bright, shrimp body is healthy and strong, trip
It is dynamic fast and strong, resistance against diseases enhancing.During nursing, the feed coefficient of prawn reduces in experimental group, and disease resistance is good, and growth is fast,
Aquaculture cost is reduced, saves manpower, material resources.Because the additive of the present invention is biological products, so not having to prawn growth
There is pollution, maintain the original abundant nutrition of prawn and delicious food.Meanwhile Chinese caterpillar fungus culture medium bacteria residue makees prawn feed additive,
Realize the recycling of resource, reduce the production cost of feed, at the same also solve Chinese caterpillar fungus culture medium residue discarding after
The problem of polluting environment.What deserves to be explained is on the premise of based on above-mentioned design, to solve same technical problem, even in
Some made in the present invention without substantial change or polishing, the essence of used technical scheme still with the present invention one
Sample, therefore it should also be as in protection scope of the present invention.
The technology of the present invention compared with the prior art, has the following advantages.
1. rapeseed dregs contains more poisonous and harmful substance, its application in animal diets significantly limit.Such as different sulphur
The materials such as cyanate, thiocyanates, oxazolidine thione, nitrile, sinapine, tannin, phytic acid.Not only influence daily ration palatability, influence
It other nutrient utilizations, can also cause animal Thyroid Gland Swell, suppress growth of animal.By being produced in fruiting bodies of cordyceps militaris
Hot and humid sterilization treatment in journey, then greatly degraded through process, harmful materials such as cordyceps mycelium growths,
Simultaneously in Cordyceps militaris growth course, protein content also improves to a certain extent, further improves the palatability of animal.
2. still remaining the wheat not being completely converted in culture medium of Cordyceps militaris leftover bits and pieces, while also there is substantial amounts of Cordyceps Militaris
Silk is present, and not utilized containing substantial amounts of protein and several amino acids in mycelia.By implementing the present invention, become dynamic
Thing can be absorbed directly, the inexpensive feed that nutrition is comprehensive, palatability is good.
3. the production of the achievable Cordyceps militaris of the present invention, can small-scale production, metaplasia can also produce on a fairly large scale.Meanwhile
While Cordyceps militaris is continually provided, the raw material of production animal feed additive is additionally provided, is to achieve many things at one stroke；
The efficient full sourceization that the present invention realizes resource utilizes, and the leftover bits and pieces using agricultural byproducts is raw material, you can production people
Edible Cordyceps militaris, the feed addictive of prawn can be also produced, realize recycling for resource, both protect environment,
Economic benefit is improved again.
4. the production method that the present invention uses, cost of material is low, equipment and simple to operate, and large-scale production can be achieved, have
Beneficial to comprehensive utilization of Cordyceps militaris, turn waste into wealth.Whole production process mild condition, without the intake of harmful toxic matter, not only may be used
To meet the safety of produced Cordyceps militaris, the bioactivity of Cordyceps militaris bacteria residue also can be farthest kept, improves prawn
The security of feed.
5. simple production process of the present invention, program is stable, easy to control, and success rate is high, using investment of production repeatedly of the invention
It is few, take up an area less, without " three wastes " problem, be adapted to enterprise to use, the food of the existing people of its product, also functional type feed addition
Agent-fish feed additive.
Brief description of the drawings.
Fig. 1 is the Cordyceps militaris in being grown using this law.
Fig. 2 is the growth potential of prawn.
Diagram a be ingest added with this method production cordyceps sinensis mushroom dreg feed additive prawn.
It is the prawn for ingesting and adding same amount of a certain commercial feed additive to illustrate b.
Diagram c is the prawn of control feed of ingesting.
Embodiment.
The present invention is further described in detail with reference to embodiments, but is not limited to this.
Embodiment 1：The preparation of Chinese caterpillar fungus culture medium.
The formula of Chinese caterpillar fungus culture medium is divided into A liquid and the component of B solids two mixes：The component of A liquid is as follows：1000
In ml running water, sucrose 10.0~15.0 g, KH are added2PO40.3~0.5 g, MgSO40.3~0.5 g, compound dimension
Raw plain B 4, vitamin C 1.The composition of B solid portions is as follows：Wheat berry 50%, the vegetable seeds dregs of rice 15%, peanut meal 24%, do
Dried silkworm chrysalis meal 1%, rice bran 10%；The liquid-solid ratio of A liquid and B solids is 2~3：1；And be sufficiently mixed uniformly, load blake bottle, it is close
Envelope, sterilize, cooling.Wherein sterilising conditions are：121 DEG C, 1.1 MPa sterilizings, 50~60 min.Cordyceps militaris provided by the present invention
Culture medium can be used for cultivating Cordyceps militaris.
Embodiment 2：The culture of Cordyceps militaris.
The Chinese caterpillar fungus culture medium culture Cordyceps militaris prepared using embodiment, including be inoculated with, cultivate, harvesting, drying and other steps.
Specially：First it is inoculated with.Cordyceps are inoculated with the blake bottle for the Chinese caterpillar fungus culture medium prepared in super-clean bench to embodiment 1
Kind, the Cordyceps militaris spawn be Cordyceps militarys (L.) Link., liquid spawn, the Cordyceps militaris spawn it is close
Spend for 3~5 bacterium ball/mL, every blake bottle 1ml；Cultivated again：The lucifuge culture under the conditions of 25 DEG C of temperature, humidity 80~87%
5~6 days, to the full Chinese caterpillar fungus culture medium surface of cordyceps filament length；6th~25 day illumination cultivation used：Daytime 12 is small
Shi Guang light is shone, 22~25 DEG C of temperature, humidity 80~85%, and intensity of illumination is controlled in 150~200 Lx；Close within 12 hours at night
Light, 20~23 DEG C of temperature, humidity 80~83%；Ventilation 2 times daily, once in the morning and once at night, 30 minutes every time；Use within 26th~45 day
Illumination cultivation：12 hours daytimes, natural light added fluorescent lamp illumination, 22~25 DEG C of temperature, humidity 80%, total intensity of illumination control
In 1100 Lx；12 hours black outs at night, 20 DEG C of temperature, humidity 75~85%；Ventilation 2 times daily, once in the morning and once at night, every time 30
Minute；Light source used in the culture of this step is scattering light, and regularly carrying out rolling bottle to blake bottle in incubation makes uniform illumination；
Harvest and be further cultured for again：Fruiting bodies of cordyceps militaris ripe in blake bottle after culture is taken out；Finally dry：By the Cordyceps militaris of harvest
Fructification is placed in the stainless steel disc for being lined with gauze, is uniformly spread out, and 30~37 DEG C of hot air circulation drying, its moisture control exists
Less than 10%.
Points for attention of the Cordyceps militaris in culture.
A. scattering light is used during illumination, and direct light can not be used, the growth of cordyceps sinensis has phototropism, to the place of uniform illumination
Bottle to carry out rolling bottle, make its uniform illumination, fructification is grown up.
B. strict temperature control and humidity are wanted in incubation, so strictly to control temperature.Do not have in current workshop
In the case of controlling temperature device, the temperature of culturing room is controlled with ground watering, sprinkles water 3 times, but also wants root within general one day
Become according to the temperature situation on the same day, temperature height will be spilt more, and culture room temperature is more than 25 DEG C, and turn on the aircondition cooling.
C. daily culturing room wants ventilation one to twice, selection of time in the morning with dusk temperature it is relatively low when,
20~30 minutes every time.
D. the bottle polluted after being inoculated with will timely be taken out, and the culture medium of the inside be poured out renewed vaccination after sterilizing again,
The bottle of pollution can not be allowed to be placed in culturing room to cultivate, and cause whole culturing room to be polluted by miscellaneous bacteria.
The Cordyceps militaris of this method culture, under field conditions (factors), it can harvest for two seasons within 1 year；, can be real such as under the conditions of batch production
Existing whole year production.Using the art of this patent, 20% yield of the output increased of Cordyceps militaris.
Embodiment 3：It is a kind of using Chinese caterpillar fungus culture medium residue as feed formula of raw material and preparation method thereof.
The invention discloses, it is higher to solve existing feed cost, and the problem of prawn quality can not be improved well.This hair
Bright prawn feed additive be by Chinese caterpillar fungus culture medium bacteria residue powder, in it containing mycelium powder of sinensis, the rapeseed meal that does not utilize, peanut
The dregs of rice and rice bran, vitamin mixtures, trace element etc..It make use of harvesting fruiting bodies of cordyceps militaris remaining afterwards in the component of the present invention
This production waste material of solid culture medium residue, sufficiently will production waste recovery utilize, greatly reduce the production of feed
Cost.
Embodiment 4：The influence that Cordyceps militaris bacteria residue is ingested to prawn, grown.
2013, on Yantai City Mouping horse keeping island, side by side 4 shrimp-cultivation pools of 2 mu of area are selected, are carried out respectively
Random experiment, it is as a result as follows.
The influence that the Cordyceps militaris bacteria residue of table 3 is ingested to prawn, grown.
| Group | 1 control | 2 Cordyceps militaris bacteria residue dry powder（5%） | 3 Cordyceps militaris bacteria residue dry powder（10%） | 4 Cordyceps militaris bacteria residue dry powder（20%） |
| Rate of body weight gain（%） | 74.35±2.3 | 78.0±1.7 | 83.13±1.9 | 70.45±3.5 |
| Survival rate（%） | 87.14±1.5 | 88.25±1.0 | 95.17±3.2 | 78.34±2.3 |
Note：The different English Superscript letters in angle represent that there were significant differences in same row（P<0.05）.
From table 3 it can be seen that 5%, 10% and 20% Cordyceps militaris bacteria residue dry powder replacement peanut meal is added when feed can influence
The rate of body weight gain of prawn（P<0.05）, cost is reduced so as to reach, the purpose to turn waste into wealth；But add 5% and 10% Cordyceps militaris bacteria residue
Dry powder is positive to the rate of body weight gain of prawn.20% and the Cordyceps militaris bacteria residue dry powder of the above be negative to the rate of body weight gain of prawn, survival rate
To.Therefore, 10-15% addition is remarkably improved the survival rate of prawn and has higher economic benefit（P<0.05）.
Claims (7)
- A kind of 1. method for producing fruiting bodies of cordyceps militaris and its bacteria residue for fish feed additive, it is characterised in that Cordyceps militaris （Cordyceps militaris）Culture medium is mixed by A liquid and the component of B solids two：The component of A liquid is as follows：1000 In ml running water, sucrose 10.0~15.0 g, KH are added2PO40.3~0.5 g, MgSO40.3~0.5 g, compound dimension Raw plain B 4, vitamin C 1；The composition of B solid portions is as follows：Wheat berry 50%, the vegetable seeds dregs of rice 15%, peanut meal 24%, do Dried silkworm chrysalis meal 1%, rice bran 10%；The solvent and solute weight ratio of A liquid and B solids is 2~3：1；And be sufficiently mixed uniformly, load blake bottle, Sealing, sterilize, cooling.
- 2. Chinese caterpillar fungus culture medium provided by the present invention can be used for obtaining fruiting bodies of cordyceps militaris and production fish feed additive.
- 3. the Cordyceps militaris being inoculated with the Chinese caterpillar fungus culture medium according to claim 1,2, is liquid spawn, it is characterised in that The method that cordyceps militaris link bacterial strain employs third stage culture, that is, it is divided into actication of culture, prepared by parent species, seed Spawn incubation.
- 4. according to the method for claim 3, after seed Spawn incubation, it is characterised in that the density of Cordyceps militaris spawn is 10~20 bacterium ball/ml, it is 1 by the volume ratio of the Cordyceps militaris seed strain of this feature and culture medium：50 mixing, carry out solid-state hair Ferment.
- 5. according to the method for claim 4, carry out the culture of Cordyceps militaris word entity, it is characterised in that the production method Comprise the following steps：First carry out light culture：The lucifuge culture 5~6 days under the conditions of 25 DEG C of temperature, humidity 80~87%, to Cordyceps militaris Mycelia covers with the Chinese caterpillar fungus culture medium surface；Carrying out illumination cultivation, the 6th~25 day illumination cultivation used：Daytime 12 is small Shi Guang light is shone, 22~25 DEG C of temperature, humidity 80~85%, and intensity of illumination is controlled in 150~200 Lx；Close within 12 hours at night Light, 20~23 DEG C of temperature, humidity 80~83%；Ventilation 2 times daily, once in the morning and once at night, 30 minutes every time；Finally, 26~45 Its illumination cultivation used, 12 hours daytimes, natural light added fluorescent lamp illumination, 22~25 DEG C of temperature, humidity 80%, and total illumination is strong Degree control is in 1100 Lx；12 hours black outs at night, 20 DEG C of temperature, humidity 75~85%；Ventilation 2 times daily, once in the morning and once at night, 30 minutes every time；The Cordyceps militaris bacteria residue after Cordyceps militaris word entity is finally harvested, drying, is applied in fish feed additive, Especially in prawn feed；The Cordyceps militaris bacteria residue after Cordyceps militaris word entity is harvested, drying, is applied in fish feed additive In.
- 6. according to the method for claim 5, Cordyceps militaris bacteria residue is obtained, routine is made an addition to using Cordyceps militaris solid-state bacteria residue dry powder In fish mixed feed, and through fully mixing, the coefficient of variation≤8%.
- 7. application according to claim 6, the addition of the bacteria residue of Cordyceps militaris in fish feed is 10~15%.
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