CN107624503A - It is a kind of to produce fruiting bodies of cordyceps militaris and fish feed additive method simultaneously - Google Patents

Abstract

The invention discloses a method for simultaneously producing fruit bodies of Cordyceps militaris and fish feed additives. The formula of the culture medium for producing Cordyceps militaris fruiting bodies is made by mixing liquid A and solid B: the components of liquid A are as follows: 1000 ml water, 10.0-15.0 g sucrose, 0.3-0.5 g KH ₂ PO ₄ , 0.3-0.5 g MgSO ₄ , 4 tablets of complex vitamin B, 1 tablet of vitamin C. The solid composition of B is as follows: 50% wheat grains, 15% rapeseed meal, 24% peanut meal, 1% dried silkworm chrysalis powder, and 10% rice bran; the ratio of A liquid to B solid powder is 2-3:1; the culture medium is sealed , sterilized, and cooled; then inoculate Cordyceps militaris strains, the density of the strains is 10-20 bacterial balls/mL, and the inoculation is carried out according to the volume ratio of the strains to the medium as 1:50. Light culture is carried out; fruiting bodies and fungus residues are obtained. The crude polysaccharide contained in the fungus residue reached 27.43%. After natural drying or drying, the fungus residue is crushed to a particle size of 80 meshes, which is beneficial to the digestion and absorption of the fish body, and has a pleasant smell. It can be used as a fish feed additive, and the addition amount is 10-15%.

Description

A method for simultaneously producing fruiting bodies of Cordyceps militaris and fish feed additives

technical field

The invention relates to the cultivation technology of Cordyceps militaris and the field of production of fish feed additives, in particular to a formulation of a medium for Cordyceps militaris and the application of the fungus residue after producing the fruiting bodies of Cordyceps militaris in fish feed and feed additives.

Background technique

Cordyceps militaris ( Cordyceps militaris ), also known as Cordyceps militaris, belongs to the Ascomycotina, Ergotaceae, and Cordyceps of the fungal kingdom. The fruiting body of Cordyceps militaris has similar medicinal and nourishing effects to Cordyceps sinensis, and the content of cordycepin in Cordyceps militaris is dozens of times higher than that of traditional Cordyceps sinensis. It is a precious medicinal fungus. Cordyceps militaris is rich in protein, amino acid and more than 30 kinds of trace elements needed by the human body. It also contains unique physiologically active components such as cordycepin and cordycepic acid, which have anti-tumor effects. Modern medical research has proved that the nutrients in Cordyceps militaris have the effects of nourishing the lungs and kidneys, stopping bleeding and reducing phlegm, expanding the trachea, calming, antibacterial, lowering blood pressure, anti-oxidation, protecting the liver, and enhancing immunity. In 2009, the Ministry of Health has approved Cordyceps militaris as a new resource food. There are many patents on the artificial medium of Cordyceps militaris, such as application number: 201310462899.4, a medium for Cordyceps militaris and its application, the medium used is rice and militaris powder; another example, application number: 201510397546.X, A medium for cultivating Cordyceps militaris and a preparation method thereof. The medium used is grain and dried silkworm chrysalis; there is no production of Cordyceps militaris using rapeseed meal and peanut meal as auxiliary materials.

Artificially cultivating Cordyceps militaris with rice and silkworm chrysalis powder is a new technology developed in recent years. After the fruiting body is harvested, the culture medium residue covered with mycelium is discarded as waste, causing serious waste of resources and environmental pollution. Cordyceps fungi residue (Edible Fungi Residue, EFR) is the culture medium residue left after the edible fungi fruit bodies are harvested during the cultivation of Cordyceps edible fungi. The amount of edible and medicinal fungus residue produced in my country is also amazing. The fungus residue is very difficult to degrade in the natural environment, and it is easy to breed mold, pests, and damage the environment, while burning will produce waste gas and pollute the environment. Doing so not only wastes valuable biological resources, but also causes significant environmental problems, so it is necessary to develop and utilize them rationally and realize the recycling of fungal residues. This patent is developed aiming at the fungus residue of Cordyceps fungus as a feed resource.

After harvesting the fruiting body, the dry weight of each bottle of discarded medium residue is about 40g, and the content of cordycepin contained in it is about 0.5%, the total sugar content is about 21.93%, the protein content is about 10%, and the cordyceps polysaccharide The content is about 12.86%. In addition, it also contains active ingredients such as alkaloids, terpenoids, sterols, glycosides, phenols, enzymes, vitamins, and SOD, which still have great utilization value. Cordycepin has the functions of antibacterial, antitumor, antiviral, and antagonizing oxygen free radicals. It has high medicinal value and wide range of pharmacological effects. If it is applied to the feed formula, it can make full use of various nutrients in the culture medium residue, and can reduce the feed cost and achieve the effect of turning waste into wealth.

Compared with the raw materials used to make the original edible fungus medium, the nutrient composition of the fungus residue has changed, the nutritional value has been significantly improved, the crude fiber has been degraded, and the content has been greatly reduced. Correspondingly, the relative proportion of protein has increased. Therefore, this fungus Slag is also known as mycofur protein. After the fungus residue is transformed by edible fungi, not only the effective nutritional content is increased, but if it is used in the feed industry, the digestion and utilization rate is greatly improved due to the degradation of crude fiber, and its palatability is increased.

With the improvement of people's living standards, people's demand for aquatic products and quality requirements have also increased. Feed is an important factor affecting the healthy growth of fish and the quality of aquatic products. On the one hand, the research and development of most prawn feeds now mainly focus on the production speed and fish output of prawns, while ignoring the improvement of their quality. On the other hand, the proportion of corn, wheat, soybean meal, etc. in the traditional feed formula is relatively high. With the increasing price of food, the cost of feed is getting higher and higher, which seriously affects the economic benefits of aquaculture.

Fish, especially prawns, have high nutritional value. They contain a variety of proteins, and the meat is soft, easy to digest, and rich in minerals (calcium, phosphorus, and iron). They are very popular among consumers. China is the second largest producer and fourth largest exporter of shrimp products in the world, and prawns are one of the main shrimp products. Small fluctuations in the production and quality of prawns will have a greater impact on aquatic product trade. The market price of prawns is relatively high, but they are prone to shrimp diseases, and the risk of breeding is relatively high. If prawns get sick or even die during the breeding process, the quantity and quality of prawns sold will be greatly reduced.

The quality of fish feed is a key factor affecting the aquatic ecosystem, fish disease resistance and quality. Taking prawns as an example, the cost of prawn feed accounts for 30% to 40% of the cost of prawn farming, which is the largest cost item in prawn farming (Duan Yuanyuan, Research on the International Trade Structure and Competitiveness of Chinese Shrimp Products [D]. Qingdao: Ocean University of China , 2009), it can be seen that the shrimp feed has become one of the important factors that determine the economic benefits of shrimp farming. In prawn compound feed, adding polysaccharides and immune enhancers are more commonly used. In the production of feed additives by fungi of the genus Cordyceps, there is an existing application number: 200710022923.7, Cordyceps activation and strengthening immune feed additives and its production method. The patent uses Cordyceps militaris and Cordyceps sinensis strains to ferment corn, soybean meal, etc., and use them as animal feed additives after fermentation. Another example is the patent application number: 97107712.6, Cordyceps fungus feed additive and its production method. This patent is to use starch to produce meat animal feed additive. None of the above-mentioned patents mentions the use of wheat grains and peanut meal to produce the fruiting body of Cordyceps militaris, and to apply its fungus residue in fish feed. Therefore, after searching, there is no use of wheat and peanut meal as the medium to produce Cordyceps militaris, and there is no use of the fungus residue of Cordyceps militaris as a fish feed additive, and there is no relevant patent on the application of this feed additive in prawns. and literature reports.

Contents of the invention

The object of the present invention is to provide a kind of culture medium formula for producing Cordyceps militaris and feed additive and preparation method thereof with the fungus slag of culture medium of Cordyceps militaris as raw material, can solve the problems such as existing prawn feed cost is high and prawn disease resistance is poor, Reduce the economic burden on fishermen.

The formula for the production of Cordyceps militaris culture medium is made by mixing two components: liquid A and solid B: the components of liquid A are as follows: 10.0-15.0 g of sucrose, 0.3-0.5 g of KH ₂ PO ₄ are added to 1000 ml of tap water , MgSO ₄ 0.3~0.5 g, 4 tablets of complex vitamin B, 1 tablet of vitamin C; the composition of the solid part of B is as follows: 50% wheat grains, 15% rapeseed meal, 24% peanut meal, 1% dried silkworm chrysalis powder, 10% rice bran ;The liquid-solid ratio of A liquid to B solid is 2~3:1; and fully mixed evenly, put into culture bottle, seal, sterilize, and cool. The sterilization conditions are: 121°C, 1.1Mpa for 30-45 minutes. The Cordyceps militaris culture medium provided by the present invention can be used for cultivating Cordyceps militaris (Fig. 1).

The method for cultivating Cordyceps militaris using the above-mentioned Cordyceps militaris culture medium includes the steps of inoculation, cultivation, harvesting and drying. The details are as follows: Inoculate first. Inoculate the Cordyceps militaris bacterial classification in above-mentioned Cordyceps militaris culture medium, described Cordyceps militaris bacterial classification is Cordyceps militaris (L.) Link., numbering is 3.4655, derives from China General Microbiological Culture Collection Center (China General Microbiological Culture Collection Center, CGMCC). The inoculum is a liquid strain, the density of the Cordyceps militaris strain is 10-20 bacterial balls/ml, and the volume ratio of the Cordyceps militaris culture medium to the Cordyceps militaris strain is 50:1; further culture is carried out. Cultivate in the dark for 5-6 days at a temperature of 25°C and a humidity of 80-87%, until the mycelium of Cordyceps militaris is overgrown on the surface of the Cordyceps militaris culture medium; the light culture adopted on the 6th to 25th day: 12 hours of sunlight during the day , the temperature is 22-25°C, the humidity is 80-85%, and the light intensity is controlled at 150-200 Lx; the light is turned off for 12 hours at night, the temperature is 20-23°C, and the humidity is 80-83%; ventilation is done twice a day, once in the morning and at night. 30 minutes each time; the light culture adopted on the 26th to 45th day: 12 hours of natural light and sunlight during the day, the temperature is 22-25 °C, the humidity is 80%, and the total light intensity is controlled at 1100 Lx; the light is closed for 12 hours at night, the temperature is 20 °C, Humidity 75-85%; ventilate twice a day, once in the morning and evening, 30 minutes each time; the light source used in the cultivation is scattered light, and the culture bottle is rotated during the cultivation process to make the light uniform; The fruiting body of Cordyceps militaris obtained by the culture method of the prior art is increased by 20%; the content of adenosine in every 1g of fruiting body of Cordyceps militaris is 15-20 mg, the content of cordycepin is 7.5-8.0 mg, and the content of polysaccharide is 25-3 mg. Obviously higher than the Cordyceps militaris cultivated by adopting the prior art method. Finally, harvest and dry: take out the fruiting bodies of Cordyceps militaris matured in the culture bottle after the steps of culturing, and dry them under sunlight or hot air circulation; place the harvested Cordyceps militaris slag in an 80-mesh stainless steel plate, spread evenly, 40 ~ 45 ℃ hot air circulation drying, the moisture content is controlled below 10%.

The present invention uses wheat grains and peanut meal as main raw materials, rice bran as auxiliary materials, and a little dried silkworm chrysalis powder, vitamins, etc. , and then adopt a special three-stage expansion cultivation method: dark cultivation, and then different light cultivation methods in different time periods, plus scattered light as the light source, and regularly rotate the culture bottles or culture pots during the culture process. Illumination is uniform, that is, by determining the optimal Cordyceps militaris culture medium formula, optimizing the culture program as a whole, and strictly following this method to cultivate the fruiting body of Cordyceps militaris, it is found that the yield is higher than that of using the prior art medium and the prior art. The yield of fruiting bodies of Cordyceps militaris obtained by culture method increased by 20%; the content of adenosine in 1g of fruiting bodies of Cordyceps militaris was 15-20 mg, the content of cordycepin was 7.5-8.0 mg, and the content of polysaccharide was 25-3 mg, which were significantly higher than those obtained by using Cordyceps militaris cultivated by the prior art method.

Application of the fungus residue of Cordyceps militaris obtained by the method of the present invention in fish feed.

The fish feed additive of the invention is an eco-friendly green biological additive. The use of the eco-environmental protection green biological additive of the invention significantly enhances the disease resistance of prawns, and increases the life rate of shrimp seedlings and adult shrimps by 7-9% during the growth process, and the effect is obvious. The growth rate of prawns was significantly enhanced. In the comparison culture of 56 days, without increasing the amount of feed, under the same feed amount, the weight gain rate of prawns added with Cordyceps militaris dregs increased by 4.5-6.0% (Fig. 2).

The effects of the present invention are further illustrated below with comparative experiments.

1. Experimental conditions and comparative experiment groups.

The experimental seawater is underground seawater, after sedimentation and aeration, it is continuously inflated in the pool and cultured in static water. The values of ammonia nitrogen, nitrite, hydrogen sulfide, pH, and dissolved oxygen in the experimental water were all within the normal range. The water temperature is 27°C and the salinity is 28 per thousand.

The experimental field is a factory breeding greenhouse, each cement pool is (1.5×1.5×1.5), 3 cubic meters, divided into two groups of 4 cement pools. In 8 cement ponds, 200 tails of shrimp seedlings with an average body weight of 4 grams were stocked in each cement pond. The control group was fed with common prawn feed; the experimental group was fed with prawn feed added with 10%-15% of the Cordyceps militaris residue. Feed twice a day.

2. Tracking measurement and experimental results.

1. Calculation of survival rate: record the number of deaths in the two groups of parallel experimental pools every day, and obtain the average number after the calculation is completed.

2. Measuring the body weight of prawns: measure once every 14 days. In two groups of parallel experimental ponds, 5 fish were randomly selected from each fish pond (20 fish in each group), and the weight of the fish was measured with an electronic scale, and finally summed and averaged.

3. The tracking measurement time is 56 days.

4. The experimental results are listed below in the following tabular form.

Table 1 Comparison of survival rates in two parallel experiments.

Average survival rate (%) Remark control group 86 average value test group 95 average value

Table 2 Comparison of body weight in two parallel experiments (unit: g).

14 28 42 56 Remark control group 4.8 7.3 10.2 15.2 average value test group 5.6 8.7 13.7 18.6 average value

3. Experimental conclusion.

Through rigorous and conscientious experiments, the environmental protection green biological additive of the present invention has remarkable effects in improving the survival rate, growth speed and the like in the application of the prawn farming technology. After observation, it was found that the prawns in the experimental group were bright in color, strong in body, fast and powerful in swimming, and enhanced in disease resistance. During the feeding period, the feed coefficient of the prawns in the experimental group was reduced, the disease resistance was good, and the growth was fast, which reduced the cost of breeding, and saved manpower and material resources. Since the additive of the present invention is a biological product, there is no pollution to the growth of prawns, and the original rich nutrition and delicious taste of prawns are maintained. At the same time, the bacterial residue of the Cordyceps militaris medium is used as a feed additive for prawns, which realizes the recycling of resources, reduces the production cost of the feed, and also solves the problem of environmental pollution after the residue of the Cordyceps militaris medium is discarded. It is worth noting that, based on the premise of the above design, in order to solve the same technical problem, even if some insubstantial changes or polishes are made on the present invention, the essence of the technical solution adopted is still the same as the present invention, so It should also be within the protection scope of the present invention.

Compared with the prior art, the technology of the present invention has the following advantages.

1. Rapeseed meal contains many toxic and harmful substances, which greatly limits its application in animal diets. Such as isothiocyanate, thiocyanate, oxazolidinethione, nitrile, sinapine, tannin, phytic acid and other substances. It not only affects the palatability of the diet and the utilization of other nutrients, but also causes animal goiter and inhibits animal growth. After the high-temperature and high-humidity sterilization treatment in the production process of Cordyceps militaris fruiting bodies, and then through the growth of Cordyceps militaris mycelium, harmful substances have been greatly degraded. At the same time, during the growth process of Cordyceps militaris, the protein content still has a certain To a certain extent, it also improves the palatability of animals.

2. In the leftovers of the Cordyceps culture medium, there still remains untransformed wheat and a large amount of Cordyceps mycelia, which contain a large amount of protein and various amino acids that are not utilized. By implementing the invention, the feed can be directly absorbed and utilized by animals, and has comprehensive nutrition and good palatability at low cost.

3. The present invention can realize the production of Cordyceps militaris, which can be small-scale production or moderate scale production. At the same time, while continuously providing Cordyceps militaris, it also provides raw materials for the production of animal feed additives, which can be said to serve multiple purposes;

The invention realizes the efficient full-source utilization of resources, uses the leftovers of agricultural and sideline products as raw materials, can produce edible Cordyceps militaris that people can eat, and can also produce feed additives for prawns, realizes the recycling of resources, and protects the environment , and increased economic efficiency.

4. The production method adopted in the invention has low raw material cost, simple equipment and operation, can realize large-scale production, is beneficial to the comprehensive utilization of Cordyceps militaris, and turns waste into treasure. The entire production process has mild conditions and no intake of toxic substances, which not only meets the safety of the produced Cordyceps militaris, but also maintains the biological activity of the Cordyceps militaris residue to the greatest extent, improving the safety of the prawn feed.

5. The production process of the invention is simple, the program is stable, easy to control, and the success rate is high. The repeated production of the invention requires less investment, occupies less land, and has no "three wastes" problems. It is suitable for enterprises to use. Its products include both people's food and functional feed Additives - fish feed additives.

Description of drawings.

Fig. 1 is to utilize this method to obtain growing Cordyceps militaris.

Figure 2 is the growth potential of prawns.

Illustration a shows the prawns fed with the Cordyceps slag feed additive produced by the method.

Diagram b shows prawns fed with the same amount of a commercial feed additive.

Panel c is the prawns fed the control diet.

detailed description.

The present invention will be further described in detail below in conjunction with the examples, but not limited thereto.

Embodiment 1: Preparation of Cordyceps militaris culture medium.

_{The formula} of Cordyceps militaris culture medium is divided into _A liquid and B solid. 0.3-0.5 g, 4 tablets of complex vitamin B, 1 tablet of vitamin C. The composition of B solid part is as follows: wheat grain 50%, rapeseed meal 15%, peanut meal 24%, dry silkworm chrysalis powder 1%, rice bran 10%; The liquid-solid ratio of A liquid and B solid is 2～3: 1; And fully Mix well, put into culture bottle, seal, sterilize and cool. The sterilization conditions are: 121°C, 1.1 MPa for 50-60 minutes. The Cordyceps militaris culture medium provided by the present invention can be used for cultivating Cordyceps militaris.

Embodiment 2: the cultivation of Cordyceps militaris.

Cultivate Cordyceps militaris using the Cordyceps militaris medium prepared in the examples, including inoculation, cultivation, harvesting, drying and other steps. Specifically: vaccination first. Inoculate the Cordyceps militaris bacterial classification in the culture bottle of the prepared Cordyceps militaris culture medium of embodiment 1 in ultra-clean bench, described Cordyceps militaris bacterial classification is Cordyceps militarys (L.) Link., liquid bacterial classification, described Cordyceps militaris bacterial classification The density of the species is 3 to 5 bacterial balls/mL, 1ml per culture bottle; further culture: at a temperature of 25°C and a humidity of 80 to 87%, culture in the dark for 5 to 6 days until the mycelium of Cordyceps militaris is overgrown. Describe the surface of the Cordyceps militaris culture medium; the light culture adopted in the 6th to 25th day: daytime 12 hours daylight light, temperature 22～25 ℃, humidity 80～85%, light intensity is controlled at 150～200 Lx; Night 12 hours closed light, Temperature 20-23°C, humidity 80-83%; ventilate twice a day, once in the morning and evening, 30 minutes each time; light culture adopted on the 26th to 45th day: 12 hours of natural light and sunlight during the day, temperature 22-25°C, The humidity was 80%, and the total light intensity was controlled at 1100 Lx; the light was closed for 12 hours at night, the temperature was 20 °C, and the humidity was 75-85%; ventilation was performed twice a day, once in the morning and evening, each time for 30 minutes; the light sources used in this step were Scattering light, during the cultivation process, the culture bottle is regularly rotated to make the light uniform; re-harvesting and re-cultivation: take out the mature fruiting body of Cordyceps militaris in the culture bottle after cultivation; final drying: put the harvested fruiting body of Cordyceps militaris on the mat Spread it evenly in a stainless steel pan with gauze, and dry it with hot air circulation at 30-37°C, and keep the moisture content below 10%.

Matters needing attention in cultivation of Cordyceps militaris.

A． Scattered light should be used for illumination instead of direct light. The growth of Cordyceps is phototropic, and the bottles in places with uniform illumination should be turned to make the illumination uniform and the fruiting bodies grow upward.

B． During the cultivation process, the temperature and humidity should be strictly controlled, so the temperature should be strictly controlled. In the current situation where there is no temperature control equipment in the workshop, the temperature of the cultivation room should be controlled by sprinkling water on the ground, generally three times a day, but it should also be changed according to the temperature of the day. ℃, turn on the air conditioner to cool down.

C． The cultivation room should be ventilated once or twice a day, and the time is selected in the morning and evening when the temperature is relatively low, 20 to 30 minutes each time.

D． After the inoculation, the contaminated bottle should be taken out in time, and the culture medium inside should be poured out and re-sterilized before re-inoculation. The contaminated bottle should not be placed in the cultivation room for cultivation, which will cause the entire cultivation chamber to be polluted by miscellaneous bacteria.

The Cordyceps militaris cultivated by the method can be harvested twice a year under natural conditions; as under factory conditions, annual production can be realized. Using this patented technology, the yield of Cordyceps militaris has increased by 20%.

Embodiment 3: A kind of feed formulation and its preparation method using Cordyceps militaris culture medium residue as raw material.

The invention discloses that it solves the problems that the existing feed cost is high and the quality of prawns cannot be improved well. The prawn feed additive of the present invention is composed of fungus slag powder of Cordyceps militaris culture medium, which contains Cordyceps mycelium powder, unused rapeseed meal, peanut meal and rice bran, vitamin mixture, trace elements and the like. The components of the present invention utilize production wastes such as solid medium residues remaining after harvesting the fruiting body of Cordyceps militaris, fully recycle the production wastes, and greatly reduce the production cost of the feed.

Example 4: Effects of Cordyceps militaris residues on shrimp feeding and growth.

In 2013, in Yangma Island, Mouping, Yantai City, Shandong Province, 4 side-by-side shrimp ponds with an area of 2 mu were selected, and random experiments were carried out respectively. The results are as follows.

Table 3 Effects of Cordyceps militaris residue on shrimp feeding and growth.

group 1 control 2 Cordyceps militaris powder (5%) 3 Cordyceps militaris powder (10%) 4 Cordyceps militaris powder (20%) Weight gain (%) 74.35±2.3 78.0±1.7 83.13±1.9 70.45±3.5 Survival rate (%) 87.14±1.5 88.25±1.0 95.17±3.2 78.34±2.3

Note: Different English superscript letters in the upper corner of the same column indicate significant differences (P<0.05).

It can be seen from Table 3 that when 5%, 10% and 20% of the dry powder of Cordyceps militaris fungus residue is added to the feed instead of peanut meal, the weight gain rate of prawns can be affected (P<0.05), so as to reduce costs and turn waste into treasure. Purpose; however, adding 5% and 10% dry powder of Cordyceps militaris has a positive effect on the weight gain rate of prawns. The weight gain rate and survival rate of prawns with 20% or more of the dry powder of Cordyceps militaris were negative. Therefore, adding 10-15% can significantly improve the survival rate of prawns and have higher economic benefits (P<0.05).

Claims (7)

1. A kind of 1. method for producing fruiting bodies of cordyceps militaris and its bacteria residue for fish feed additive, it is characterised in that Cordyceps militaris （Cordyceps militaris）Culture medium is mixed by A liquid and the component of B solids two：The component of A liquid is as follows：1000 In ml running water, sucrose 10.0~15.0 g, KH are added₂PO₄0.3~0.5 g, MgSO₄0.3~0.5 g, compound dimension Raw plain B 4, vitamin C 1；The composition of B solid portions is as follows：Wheat berry 50%, the vegetable seeds dregs of rice 15%, peanut meal 24%, do Dried silkworm chrysalis meal 1%, rice bran 10%；The solvent and solute weight ratio of A liquid and B solids is 2~3：1；And be sufficiently mixed uniformly, load blake bottle, Sealing, sterilize, cooling.
2. 2. Chinese caterpillar fungus culture medium provided by the present invention can be used for obtaining fruiting bodies of cordyceps militaris and production fish feed additive.
3. 3. the Cordyceps militaris being inoculated with the Chinese caterpillar fungus culture medium according to claim 1,2, is liquid spawn, it is characterised in that The method that cordyceps militaris link bacterial strain employs third stage culture, that is, it is divided into actication of culture, prepared by parent species, seed Spawn incubation.
4. 4. according to the method for claim 3, after seed Spawn incubation, it is characterised in that the density of Cordyceps militaris spawn is 10~20 bacterium ball/ml, it is 1 by the volume ratio of the Cordyceps militaris seed strain of this feature and culture medium：50 mixing, carry out solid-state hair Ferment.
5. 5. according to the method for claim 4, carry out the culture of Cordyceps militaris word entity, it is characterised in that the production method Comprise the following steps：First carry out light culture：The lucifuge culture 5~6 days under the conditions of 25 DEG C of temperature, humidity 80~87%, to Cordyceps militaris Mycelia covers with the Chinese caterpillar fungus culture medium surface；Carrying out illumination cultivation, the 6th~25 day illumination cultivation used：Daytime 12 is small Shi Guang light is shone, 22~25 DEG C of temperature, humidity 80~85%, and intensity of illumination is controlled in 150~200 Lx；Close within 12 hours at night Light, 20~23 DEG C of temperature, humidity 80~83%；Ventilation 2 times daily, once in the morning and once at night, 30 minutes every time；Finally, 26~45 Its illumination cultivation used, 12 hours daytimes, natural light added fluorescent lamp illumination, 22~25 DEG C of temperature, humidity 80%, and total illumination is strong Degree control is in 1100 Lx；12 hours black outs at night, 20 DEG C of temperature, humidity 75~85%；Ventilation 2 times daily, once in the morning and once at night, 30 minutes every time；The Cordyceps militaris bacteria residue after Cordyceps militaris word entity is finally harvested, drying, is applied in fish feed additive, Especially in prawn feed；The Cordyceps militaris bacteria residue after Cordyceps militaris word entity is harvested, drying, is applied in fish feed additive In.
6. 6. according to the method for claim 5, Cordyceps militaris bacteria residue is obtained, routine is made an addition to using Cordyceps militaris solid-state bacteria residue dry powder In fish mixed feed, and through fully mixing, the coefficient of variation≤8%.
7. 7. application according to claim 6, the addition of the bacteria residue of Cordyceps militaris in fish feed is 10~15%.