CN112956591A

CN112956591A - Extraction of plant protein from herbivore and preparation of coarse protein feed of herbivore

Info

Publication number: CN112956591A
Application number: CN202110242663.4A
Authority: CN
Inventors: 叶绍朋; 昌旭初; 叶成健
Original assignee: Ye Shaoya
Current assignee: Ye Shaoya
Priority date: 2021-02-23
Filing date: 2021-02-23
Publication date: 2021-06-15

Abstract

Extracting plant protein from leaf-eating grass and preparing crude protein feed of leaf-eating grass, cutting or pulping 1000kg of leaf-eating grass into 80 mesh pieces, adding the pieces into a 2000L jacketed reaction kettle preheated to 95 ℃ hot water, stirring at the rotating speed of 10-30 r/min for 3-10 min, cooling and discharging, pumping the pieces into a plate-and-frame filter press by a pump, keeping the average pressure of the plate-and-frame filter press at 0.6MPa, continuously performing filter pressing for 8 hours, collecting the filter pressing effluent, pumping the water into another 2000L enamel jacketed reaction kettle by the pump, stirring and cooling to normal temperature, dropwise adding 20% NaOH solution into the reaction kettle, and dropwise adding 30% H solution into the reaction kettle₂O₂Decoloring the solution, pumping the decolored solution into a high-speed centrifugal spray dryer by a pump, controlling the inlet air temperature of preheated air of the dryer to be 150 ℃ for discharging to obtain the plant protein of the leaf-eating grass, continuously adding the leaf-eating grass into the filter-pressed residue of the leaf-eating grass discharged from a plate-frame filter press, adding 10 percent of corn flour, adding 5-10kg of active yeast powder into one thousand kilograms of the leaf-eating grass, uniformly mixing, hermetically stacking by using a plastic film for heat preservation, naturally raising the temperature of the stack to 35 ℃, fermenting for 1-3 days for heat preservation, drying, cooling and packaging by using a 120 ℃ roller dryer to obtain the crude protein feed of the leaf-eating grass.

Description

Extraction of plant protein from herbivore and preparation of coarse protein feed of herbivore

Technical Field

Extracting plant protein from the leaf-eating grass and manufacturing a leaf-eating grass crude protein feed, wherein the leaf-eating grass plant protein is extracted by a hydrothermal method, and the leaf-eating grass, corn starch, salt, trace elements and mineral substances are continuously added into residues after the protein is extracted from the root of the leaf-eating grass for fermentation to serve as raw materials of the animal feed, namely the leaf-eating grass crude protein feed; the production of the leaf eating grass feed is matched with the solid waste utilization of the agriculture and forestry straws, so that the energy is saved, the emission is reduced, and the shortage of human food, vegetable and plant protein resources is solved; the leaf eating grass is used as a ring in a food chain, and belongs to the environmental protection field of circular economy, clean production and zero emission; the extraction of the leaf-eating grass vegetable protein by the hydrothermal method reduces the production cost, solves the problem of high cost of treatment of hazardous waste residues generated after the extraction of the leaf-eating grass vegetable protein by the chemical method, and the application unit of the leaf-eating grass vegetable protein product is compiled into leaf-eating grass vegetable protein SYC-906.

Background

In the world, the leaf-eating grass is taken as the plant protein source of human edible protein and animal feed, replaces part of grain feed and animal protein source, extracts, promotes and uses the leaf-eating grass protein, and has just started to become the industrial popularization of agricultural industrialization scale in China 'fourteen five' as the happy village. The leaf-eating grass and the agricultural straw are fermented into the composite protein feed, the problem of agricultural straw returning or burning pollution is solved, and a leaf-eating grass protein fiber feed product is not available in the world, so that the leaf-eating grass protein fiber feed belongs to a new industry. The leaf-eating grass is a hybrid variety of a new species development research institute of the former Soviet Union agricultural institute, which uses Rumex native to Russia as a mother line and Rumex madaio wild plant of Heilongjiang, commonly known as Rheum palmatum L, Rumex japonicus leaf, Stephania delavayi Franch, xuelian, saussurea involucrata and fresh Daqing of China as a father line, and is a plant of Rumex of Polygonaceae. The plants from wild to artificial cultivation, which are bred and domesticated by a sexual hybridization method, are named as the herbivores, also called as proteinacious grass, herbivorous vegetables, proteinacious grass, amino acid grass and amino acid vegetable. The perennial root herbaceous plants are planted once, can be harvested in 30-40 years, and yield per mu is 30 tons per year, so that the yield is high; the leaf eating grass root can improve soil, the nutrient contents of leaf protein, trace elements, amino acid and the like are higher than those of common vegetables, after processing, the palatability is good, the nutrition is rich, the leaf eating grass root is suitable for developing and replacing part of grains, and can be used for preparing human protein food vegetables, extracting medicines and animal feeds and reducing the grain consumption; the herba Lysimachiae Christinae root can be used for extracting flavonoid, cooking soup with medicated diet, and making wine; planting the leaf eating grass, improving the ecological environment of soil, treating soil organic matter pollution, harvesting and manufacturing fertilizer, and recycling; the leafy grass plant protein product and the cellulose product are processed to replace partial grain and vegetable food, manufacture health care products, picky food, manufacture leafy grass feed and feed various herbivorous and carnivorous animals of human beings, and the leafy grass plant protein product and the cellulose product are used as the feed for replacing partial grain crops to manufacture medicaments, so the leafy grass plant protein product and the cellulose product have wide market popularization and development application prospects.

The dried leaf eating grass contains plant protein about 60 percent, and the fresh and dried products also contain vitamins, eighteen amino acids, SOD, isoflavone, chlorophyll, chlorogenic acid, trace potassium, selenium, calcium, magnesium and other beneficial elements and mineral substances, and is a novel high protein plant which is used for developing grain-saving human edible plant protein food and producing animal plant protein feed; the leaf eating grass male parent soil oxtongue planting is matched with other insecticidal plant planting, the leaf surface insecticide and soil insecticidal fertilizer used for producing the leaf eating grass are harvested and used as a biological pesticide, the residue of a chemical pesticide used for producing the leaf eating grass is reduced, and the pure ecological plant leaf eating grass is produced to produce edible plant protein and animal feed, so that the grain food production and animal feeding cost is reduced, the grain is saved, the meat, egg and milk quality of livestock and poultry is improved, and the food supply of human beings and the healthy breeding of the animal husbandry are ensured. The leafy grass is a natural human plant protein resource, is used for producing leafy grass plant protein milk powder or leafy grass plant protein milk beverage, develops to partially replace natural milk product protein, is used in the fields of grains, foods, health care products and the like, increases human nutrition, improves disease resistance rate and improves human life quality. Planting the leaf-eating grass, treating soil pollution, absorbing carbon dioxide, increasing oxygen, purifying atmospheric environment, adjusting climate and reducing carbon emission.

The leaf-eating grass is perennial herbaceous plant, leaf-eating grass, named proteinic grass, amino acid grass and longevity grass. Boundary: the plant kingdom. A door: angiosperm. Class: dicotyledonous plants. Mesh: polygonum order. Family: polygonaceae family. Subfamily: rumex acetosa subfamily. Family (c): and (4) an Rumex acetosa family. Belongs to the following steps: rumex genus. Subgenus: malachite subgenus. The method comprises the following steps: sorrel. Distribution area: jiangsu, northeast, northwest, Shandong, Henan, Hebei, etc. The main root is developed, the stem is upright and does not branch, and lateral buds grow on the root and stem; tufted, smooth full-edge, green-green leaf, acicular oblong, 49-83 cm long, 9.8-34 cm wide, 19-59 cm long petiole; the stem tip of the early summer grows pale green transparent pink white florets, a conical inflorescence, 7 to 8 stamens and a female columella is reddish purple; the fruits are wrapped with wing-shaped preserved sepals, the trigone type small nuts are yellow brown, the dropping property is strong, and the weight of about 380 seeds is 0.9 g. The main root of the leaf-eating grass is thick and big, has overwintering capability, and has upright and thick stem with arris groove. The stem before the node is pulled out is contracted at the ground surface, and the leaves are overlapped into a lotus socket shape; after harvesting, the overground part of the plant is dried and died, the embryo at the root begins to grow to hibernation, and after the plant is hibernated through winter, new leaves grow at the top of the root for the second vegetative growth. The leaves die on the ground in winter, a plurality of overwintering buds are formed in the ground, and the leaves grow green in the spring of the next year. The leaf eating grass can continuously grow for decades once, the service life is long, the yield is high, about 25 tons of fresh grass can be produced per mu, the yield also reaches over 38 tons, and the yield is low and related to soil, water sources, fertilizers, field management and harvesting times. The leaf eating grass is harvested for a certain time according to needs, and is generally harvested once in about 16 to 28 days under the condition of sufficient base fertilizer and water. And planting and harvesting the plants in the south all the year round. The leaf-eating grass not only has the characteristics of high yield, fast growth and excellent quality, but also has strong cold resistance, can resist low temperature of-40 ℃ after the root is strengthened, has the characteristics of drought resistance, waterlogging resistance, alkali resistance, barren resistance, wide adaptability, strong anti-adversity regeneration capability and the like, is suitable for being planted in saline soil, can grow in soil with the pH value of 5-9.4 and the salt content of 0.3-0.6%, and is not limited by mountainous regions and fields for planting. The leaf-eating grass grows slowly in high-temperature seasons at the temperature of more than 34 ℃, and shade-shielding measures should be taken in time in high-temperature seasons to keep watering irrigation. The leaf-eating grass enters a half withering period in 12 th of the north, turns green in 4 months in the year, and leaves of the root part regenerate after the green turning, and thrive in spring. The saline-alkali tolerant herbivore can normally grow in saline-alkali soil and sandy wasteland, and most crops cannot normally grow when the salt content in the soil exceeds 0.3%. The saline-alkali resistant characteristic of the method has wide planting application prospect for fully developing, utilizing and transforming coastal saline-alkali soil in China. Cold resistance and drought resistance of the leaf-eating grass: is cultivated in the area above-35 ℃ in northern latitude, has "innate" adaptability to cold, can safely overwinter under the condition of-39 ℃, has strong cold resistance and turns green after snow melting; the root system of the leaf-eating grass can be as deep as about 1 meter, the water in deep soil can be fully utilized, the leaf-eating grass can still grow in the area with annual rainfall of 130 mm, the hot air drought death caused by less rainfall can be effectively resisted, and the leaf-eating grass is most suitable for being planted in dry and waterlogged areas which depend on more people and eat more people, depends on human economy, and solves the problems of the aging of the current rural population, the loss of agricultural labor force and the embarrassment of no human planting field; the leaf-eating grass is a hydrophilous plant, the ditch edge and the river edge polluted by eutrophication are provided with sufficient water source fertilizer, namely the leaf-eating grass grows fast and strong, the yield and the quality are high, the leaf-eating grass is planted, biological pesticide and Chinese herbal medicines are used for interplanting and weeding, and chemical pesticide and herbicide are not needed to be sprayed. Therefore, the ecological protection method is more suitable for being planted in ecological protection red lines of economic zones of the Yangtze river along the Yangtze river, is interplanted with insect-resistant plants such as wild rape and leaf mustard and can replace high and large trees with low value, fix sand, protect slopes, absorb nitrogen, phosphorus and organic pollutant fertilizers and improve the ecological environment of the water quality of the Yangtze river. Because the yield of the leaf-eating grass is high: the growth capacity is strong, the fresh grass is harvested in spring, summer and autumn in the north, and if the fresh grass is harvested under the conditions of water, fertilizer and good management, about 20 to 39 tons of fresh grass is produced per mu in the year; the yield is higher when the crops are cut all the year round in south. The method is suitable for mountain area corner plots, hilly lands, desert lands, saline-alkali soil, low-lying flood zones, flood discharge channels, under road and railway elevated floors, polluted plot management and agriculture poverty-removing and richening-causing special industries of inland poverty areas with more people and less land: a county-village-one product is prepared into characteristic industries such as phyllophagous grass plant protein powder, phyllophagous grass plant protein milk beverage, phyllophagous grass old jar pickled vegetables, phyllophagous grass plant protein health care grass-like golden wine flavone wine, phyllophagous grass pickled vegetables and a series of phyllophagous grass Chinese medicinal material products, phyllophagous grass feed products and paternal native rhubarb phylloxera insecticidal fertilizer products, becomes to utilize the phylloxera series products to drive other related agriculture and forestry cycle industry development, forms large-scale agricultural economy, assists the Chinese government, and 'fourteen five' revitalizes the country, develops the economy, drives farmers to be rich together, and the policy and fund support of committee of the central government should be obtained.

The leaf-eating grass has the advantages that if the leaf-eating grass plant protein is directly extracted by chemical methods such as trichloroacetic acid and the like after the fresh grass harvested from the leaf-eating grass is cleaned, the cost is high, after all the plant protein of the leaf-eating grass is extracted, trichloroacetic acid residues are generated to be dangerous waste, the leaf-eating grass residues containing trichloroacetic acid and the like are dangerous waste and solid waste, and the treatment cost is high; the chemical method for extracting the leaf-eating grass plant protein is not cost-effective in utilization, and the utilization of the leaf-eating grass plant protein and the economic benefit are hindered. The purification development of the leaf-eating grass plant protein is hindered, and the popularization and development of replacing part of grain plants in the field of replacing part of grain plant protein and animal feed are also hindered. Causing the waste of high-quality protein resources of the leaf-eating grass.

The method is characterized in that the method comprises the steps of extracting the leaf-eating grass vegetable protein by a hydrothermal method, then preparing the leaf-eating grass vegetable protein into edible vegetable protein powder, protein beverage or milk beverage by a microbial fermentation method, extracting the leaf-eating grass residue after the leaf-eating grass vegetable protein is extracted, extracting the residue after flavone is extracted from the root of the leaf-eating grass, matching with pollution-free agricultural plant straws, and preparing the high-protein livestock and poultry feed by a heat preservation fermentation method of adding lactic acid bacteria or yeast, which is also a good method for producing grass vegetable protein to replace grain feed in livestock and poultry breeding industry. In recent years, in order to prevent straw burning, a series of burning forbidding orders are produced from place to place successively, and certain effect is achieved. Clean straw burns the electricity generation, has wasted effectual forage grass resource and has still increased the atmospheric carbon and discharge, burns the straw on the spot and will reduce soil fertility, causes the arable land barren. Most of elements such as nitrogen, potassium, sulfur, phosphorus, carbon fiber organic matters and the like contained in the straws are converted into volatile substances or particles to enter the atmosphere, only part of substances such as potassium elements and the like are reserved, the loss of nutrient elements is serious, the air pollution is serious, and the method is very not favorable for soil fertility improvement and environmental protection. According to the measurement, the soil organic matter is reduced by 0.22-0.28 percent when the straw is burned on site. If the soil organic matters are generated by returning straws to the field, the soil organic matters generally need to be continuously generated for 7-9 years. The straw burning can bring away the soil moisture and destroy the soil moisture content. Relevant experimental studies show that straw burning causes 62-76.9% of soil moisture loss, and the damage is particularly serious for northern arid regions. Burning the straw will destroy the biological community of the farmland and burn a large amount of soil microorganisms. Research shows that the number of bacteria, actinomycetes and fungi in the soil after incineration is respectively reduced by 84.31%, 76.46% and 85.3% compared with the number before incineration, and the soil of farmland is easy to harden. The burning straw can eliminate partial diseases, pests and weeds, but can also make some diseases and pests more serious. For example, after the wheat straw is burned, the incidence of pests and diseases such as caterpillar and smut is higher than that of returning the straw to the field. The experimental determination result shows that the ratio of the plant of the caterpillar disease crop after the straw burning is 21/98, which is higher than that of 13/98 under the condition of returning the straw to the field. The yield of crops growing in straw-incinerated soil is reduced. Experiments show that the total absorption area and the active absorption area of the root systems of the corn seedlings and the soybean seedlings growing in the straw burning soil are obviously reduced, and the reduction rates are respectively 22.37% and 20.87%. This indicates that the straw burning soil is not beneficial to the growth of the corn and soybean seedling root system, the activity of the root system is obviously reduced, and the normal growth and the stress resistance of the corn and soybean seedling are very unfavorable. The direct crushing and returning of the straws to the field can cause the increase of plant diseases and insect pests of next-crop plants and the reduction of soil fertility, and the additional application of chemical fertilizers is needed, and the additional application of the chemical fertilizers can cause soil hardening. Therefore, various hazards brought by burning the straws are fully known, the resource utilization of the straws is emphasized and promoted, the high-protein leaf eating grass is blended to manufacture the animal feed by utilizing a microbial fermentation method, grain resources required by livestock and poultry breeding industry can be saved, the barren food in the world is relieved, the effect is huge, the leaf eating grass can be directly blended with the straws, various livestock and poultry manure composts are matched for fermentation, then plant pesticides are blended, non-toxic solid waste organic matters such as nitrogen, sulfur, phosphorus and urea are added to be mixed to be used as biological compost, and the crushed male parent of the leaf eating grass is added during fertilization: fresh native rhubarb is added with castor oil plant, tung oil tree leaves and the like to manufacture an insect-killing and insect-expelling ecological base fertilizer organic fertilizer or a native rhubarb pesticide aiming at the phyllophagus, the native rhubarb-the gemini brothers of the phyllophagus is utilized, the insect killing and expelling of the native rhubarb of the phyllophagus is carried out in the production of the phyllophagus, the phyllus is produced in agriculture without chemical pesticide, the straw is mixed with the phyllus fermented feed, the animal manure is returned to the field for improving the soil, the agricultural straw is not required to be burnt, the phyllus plant protein is produced and extracted by the phyllus to be used as human food and the phyllus and agricultural straw feed products, the virtuous circle is realized, the phyllus industry drives the ecological agriculture development, and the product in one village is a career which benefits the millennium in the next.

As the implementation of the grain safety law, the food safety law, the environmental protection law, the atmosphere ten items, the soil ten items, the solid waste law and the circular economy law in rural areas strictly forbids to burn the straws, the straws in farmer places can only be packaged, sold, burned and treated to generate electricity, and huge animal feed food fiber and straw protein resources are wasted. The pollution-free straws are preferably treated by crushing, crushing and returning to the field, or used as fermented feed or biogas fertilizer. The biogas production needs a plurality of links to be controlled, various livestock and poultry manure, leaf eating grass resources planted on the improvement of soil pollution treatment are utilized, residue solid waste after flavone extraction from the root of the leaf eating grass is matched, soil and residual rotten vegetable leaves after the classification of municipal domestic waste are mixed with farmyard manure livestock and poultry manure and straw fermentation compost or retting fertilizer, the environment is not polluted, the problems of non-dangerous chemical organic wastewater and solid waste in industrial and agricultural production are solved, the biogas production technology is used for producing leaf eating grass fertilizer or producing non-edible pot flower park road forest directional slow-release insect expelling base fertilizer or non-insect economic forest wood and wood resources production popularization, non-insect forests for planting edible fruit oil plants and protecting road and park forest resources, and the biogas production technology is a good invention and creation.

The feed industry develops rapidly due to rapid development of the livestock and poultry breeding industry, a feed factory also allows animals to grow fast hormone feed for selling various disease-resistant fast hormone feed for a plurality of times, the animals are not easy to get ill after eating the feed, the feed factory mixes a plurality of antibiotics, heavy metals, essence, spices, salt and the like into the animal feed, the feed association does not stipulate the limit values of the heavy metals and the antibiotic content in the feed at all, antibiotics, heavy metals, pigments, spices and the like are added into the feed at will, so that the harmful components of the excrement of the bred animals are complicated and become dangerous waste, farmers apply the excrement containing the dangerous waste livestock and poultry as a fertilizer, soil pollution and agricultural plants are toxic, and the produced agricultural products do not accord with food sanitation standards, thereby causing huge economic loss and environmental pollution; the fresh livestock and poultry manure is used as an agricultural fertilizer at will, so that the seeds of vegetables, agricultural plants and the land can be polluted secondarily, and the vegetable, the agricultural plants and the seeds cannot be eaten, so that the health of the body is damaged; animal waste cannot be directly used as agricultural fertilizer and becomes dangerous waste, and the incineration and landfill cost is too high for treating the animal waste dangerous waste containing antibiotics and heavy metals; for environmental protection, governments have to ban or limit breeding to limit emissions; the majority of farmers with more than large scale need to invest and build a large-scale sewage treatment plant and a sewage treatment plant, and generate sludge to be discharged randomly or need sludge drying and burning treatment, thereby increasing the sewage treatment cost and landfill of solid waste pollution; at present, in the case of poor pollution control, the rural scale breeding industry is closed well by law, the agricultural loss is huge, the grain economic crop planting is easy to lose the cost, the land is not attractive, many farmers lose the industry, the employment pressure is high, the pork price rises, the price of paper, ammonium phosphate, compound fertilizer, potassium phosphate fertilizer, urea and ammonium bicarbonate rises, the environmental pollution and the economic downlink pressure are high, the agricultural problem is serious, the soil pollution is serious, the grain and seed safety threatens and endangers the national safety, the leaf eating grass planting special for producing the seeds is used, the sludge of a sewage treatment plant is used as the leaf eating grass fertilizer, the organic pollutants in the sludge can be absorbed, and the organic matters are converted into the energy and the nutrient for the growth of the leaf eating grass; the method is characterized in that a traditional Chinese medicine rhubarb and native water extract is utilized and matched with residue wastes obtained after flavonoid is extracted from roots of the common leafy herbs, both the extract and the residue wastes contain biological insecticides, the residue wastes are used for killing insects for the common leafy herbs or killing and expelling insects for other agricultural grain vegetables, melons and fruits, forestry or animals and poultry breeding industry animals, the livestock and poultry manure is used as an insecticidal biological fertilizer for killing insects and expelling insects by adding crushed rhubarb and other insecticidal plants during fertilization, and the livestock and poultry manure is used as an insecticidal leaf fertilizer and a base fertilizer for fertilizing the oriented fertilization on the common leafy herbs, agriculture, forestry, breeding animals, edible flowers and parks, and gardens, so that the problems of spraying highly toxic chemical pesticides on parks, roads, forests and flowers and chemical pollution on air, soil, surface water, pedestrians and vehicles are solved.

The ecological solid non-edible organic fertilizer for the insect expelling base fertilizer for the potted flower garden road forest trees is prepared by extracting agricultural plants, animals, raised animals, domestic garbage, livestock manure and agricultural straws from the roots of the leaf-eating grass and the rhubarb root and the soil, mixing the fresh soil and the rhubarb with the waste straws of the animal and livestock manure agriculture and forestry, and then preparing the ecological solid non-edible organic fertilizer for the insect expelling base fertilizer for the potted flower garden road forest trees. The method comprises the steps of treating straw and livestock manure harmless treatment resource utilization in agriculture and gardens, preparing insect-expelling ecological organic fertilizer from residue of the straw and livestock manure, killing insects and applying fertilizers to the straw and livestock manure, recycling, saving resources, treating the straw and livestock manure in agriculture and gardens, treating antibiotic-containing manure, recycling the classified municipal domestic garbage containing parasites and being capable of being used as fertilizers, treating soil organic matters, pesticides and heavy metal pollution, loosening soil hardening, promoting grain production, protecting environment and increasing employment.

The invention relates to a method for extracting plant protein from leaf-eating grass and preparing leaf-eating grass crude protein feed, which is popularized and implemented in China, and is used for preparing insect-expelling ecological organic fertilizer directional base fertilizer for garden roads and forests of non-edible potted flowers and gardens by mixing and fermenting solid waste of agricultural and garden waste straws, animal manure and leaf-eating grass residues, adding fresh native rhubarb and preparing the insect-expelling ecological organic fertilizer for the gardens and the forests of the non-edible potted flowers and gardens, and can treat 2000 ten thousand meters per year³The residue after extracting plant protein from the above-mentioned herbivorous herbs is used as solid waste. The rhubarb root is added for fertilization, agricultural and garden straw and livestock manure with the concentration of more than 3000 million cubic meters per year can be treated to kill flies, maggots and mosquitoes, solid waste is used as an ecological fertilizer, resource utilization is realized, environment is purified and discharged up to the standard, chemical fertilizer utilization produced by taking coal as a resource is reduced, natural coal resources are saved, 6000 million m can be treated³Over one year, various animal excrement solid waste sludge containing agricultural garden straws and urban and rural organic sludge can save land resources and increase soil fertility of garden trees, agricultural and garden waste straws are mixed with leaf-eating grass, leaf-eating grass roots or solid waste after protein extraction to prepare ecological organic fertilizer by fermentation, soil and rhubarb are added for fertilization, and female parent is used for fertilizingThe invention can be used for purifying and beautifying the environment, saving the treatment cost, lightening the burden of the government and farmers, improving the health level of residents, promoting the economic development, maintaining the ecological safety and maintaining the national safety, and the invention conforms to the food safety law, the national environmental protection law, the ten items of soil, the ten items of atmosphere, the ten items of water, the solid waste law, the circular economy law, the renewable energy law, the Yangtze river protection law and the like. The method is characterized in that a cyclic economy mode is adopted, from one corner of the cyclic economy of phyllanthus edulis and rumex madaio, the industrial chain of a plurality of agricultural processing products is extended, the problem of agricultural pollution in rural areas of China is solved, employment is increased, the problems of waste water treatment and solid waste treatment zero discharge of phyllanthus edulis residues in the production of extracting proteins and flavones from leaves and roots of phyllanthus edulis are solved, the utilization of generated residues when the phyllanthus edulis and the roots are used for extracting plant proteins and flavonoid Chinese medicinal materials is solved, the problems of environmental pollution, waste water treatment and solid waste incineration, large investment and resource waste are solved, the problems of national fertilizer production by using the Chinese medicinal residues or partial replacement of fermented feed are solved, and the fourteen-five national popularization can generate trillion social benefits, ecological benefits, environmental benefits and economic benefits for national society. All the production techniques and innovations for producing all the leafy grass plant protein products published in the terms of the claims and the detailed description of the invention patent are protected by the intellectual property laws.

Disclosure of Invention

Extracting plant protein from the leaf-eating grass and manufacturing the crude protein feed of the leaf-eating grass, and the method comprises the following steps: cleaning 1000kg of harvested fresh leaf eating grass with clear water, uniformly cutting into filaments with the width of 0.36-60mm on an automatic vegetable cutter or smashing into 10-60 meshes of fragments by a beater; in a 2000L-5000L jacket enamel reaction kettle or a 2000-5000L jacket 316 stainless steel reaction kettle which is heated by steam or heat transfer oil or electricity and is provided with stirring, 300-500L tap water is added in advance and stirred at 2-3 r/min and is heated to 50-97 ℃; adding 1000kg of chopped or crushed fresh herba Achilleae, stirring at 10-15 rpm, heating the liquid mixture of herba Achilleae fragment and water in the reaction kettle, and maintaining at constant temperature of 50-97 deg.CStirring for 5-20 minutes at any temperature, sampling, observing the color of the herbivore fragments in the reaction kettle to become dark and soften; opening a valve at the bottom of the reaction kettle, pumping and discharging by using a 316 stainless steel pump with the inlet and outlet caliber of DN50-100 and the lift of 25-100 m, pumping the discharged material, pressurizing and pumping the softened fragments of the leaf eating grass into a plate-and-frame filter press with the weight of 10 tons/day, keeping the average surface pressure of the plate-and-frame filter press at 0.6-0.9MPa, continuously performing filter pressing for 3-12 hours, and stopping the filter pressing; collecting all press-filtered effluent of plate-and-frame filter press, pumping into another 8-12m pump with 316 stainless steel self-sucking pump with caliber DN50-100 and lift of 50-100 m³Starting a stirrer in the enamel jacket reaction kettle or the 316 stainless steel jacket reaction kettle with the stirrer, stirring at the rotating speed of 10-20 r/min, keeping the liquid in the reaction kettle, and cooling by circulating water through the jacket until the leaf eating grass protein liquid in the reaction kettle reaches the normal temperature; adding 10-36% of food-grade NaOH solution dropwise into the protein solution filtered in the reaction kettle under the condition of continuous stirring until the pH value of the mixed solution filtered in the reaction kettle is 8-9, stopping adding the NaOH solution dropwise, and adding 10-50% of H dropwise into the mixed solution filtered in the reaction kettle under the condition of continuous stirring₂O₂The solution is sampled and observed in batches, the pressure filtration mixed solution in the reaction kettle is changed from light green to colorless and transparent, and the dropwise addition of H is stopped₂O₂Obtaining a decolorized leaf-eating grass vegetable protein preparation solution; the prepared leaf-eating grass plant protein preparation liquid is added into a feed inlet of a high-speed centrifugal spray dryer with the evaporation processing capacity of 500-; discharging the finished product of the leaf-eating grass vegetable protein with the water content of 8-15% from a discharge port of a spray dryer, packaging the finished product of the leaf-eating grass vegetable protein by using a woven bag or a kraft paper bag lined with a polyethylene plastic bag according to the weight of 25-100 kg/bag, inspecting, packaging, detecting, sealing and selling; absorbing condensate water absorbed by hot air convection from tail gas of the spray dryer as backup liquid, and storing the backup liquid at 1000m³The 316 stainless steel tank replaces tap water hot water for recycling; the filter-pressed leaf eating grass residue discharged from the plate-frame filter press is treated according to the state or the enterprise for animalsStandard requirements for feed, according to various animals: adding fresh leaf-eating grass with the mass percentage of 50% of wet substances of leaf-eating grass residues into the leaf-eating grass residues according to the requirement of the nutrient mixing proportion of all nutrient elements required by pigs, cows, horses, sheep, chickens, ducks, geese, rabbits, dogs, cats, birds, crabs in the mixed feed, adding corn flour with the mass percentage of 8-20%, wheat bran with the mass percentage of 3-15%, rice bran with the mass percentage of 3-8%, calcium hydrophosphate with the feed grade of 1-2%, cottonseed cake powder or rapeseed cake powder with the feed grade of 0.3-100 meshes, salt with the feed grade of 0.3-0.6%, diatomite with the feed grade of 0.2-0.5%, vitamin B1 with the feed grade of 20-180ppm, B2, B12, VC, A and D, adding feed grade ferrous sulfate of 0.1-0.2ppm, adding crushed corn residues or non-polluted corn straws with the mass percentage of 10-10 meshes into the leaf-eating grass residues according to the mass ratio of 1: 10 Uniformly mixing rice straws or waste grass straws in a feed mixer to form a leaf eating grass tailing protein premix; adding 1000kg of the grass-eating tailing protein premix into 5-10kg of commercially available active yeast powder according to the mass ratio of 1000kg of the grass-eating tailing protein premix, stirring and mixing for 10-20 minutes by using a 316 stainless steel screw mixer, simultaneously dripping tail gas of a spray dryer to absorb circulating water to adjust the humidity of the material, keeping the total water content of the grass-eating tailing protein premix, and keeping the grass-eating tailing protein premix to be held into a ball by hands without dripping; placing the grass-eating tailing protein premix into a factory building, placing the grass-eating tailing protein premix on the ground using granite building blocks, covering a nontoxic polyethylene plastic film on the grass-eating tailing protein premix, sealing and insulating the grass-eating tailing protein premix, naturally heating the grass-eating tailing protein premix to 30-40 ℃, insulating and fermenting, monitoring the temperature in real time, turning over and cooling when the temperature reaches 45 ℃, keeping the constant temperature of a material pile between 38 and 40 ℃, continuously fermenting for 1-3 days, and stopping fermentation to obtain the grass-eating tailing protein feed; naturally cooling the fermented leaf-eating grass tailing protein premix to room temperature, directly heating to 105 ℃ for 3 minutes, feeding animals such as pigs, cows, horses, sheep, rabbits, chickens, ducks and geese after cooling or drying and packaging at 120 ℃ by using a drum dryer to obtain dry leaf-eating grass crude protein feed; according to 25-100 kg/bag, using inner lining polyethylene plastic bag and outer package braidingAnd packaging the fermented and dried crude protein feed finished product of the leaf-eating grass by woven bags or kraft paper bags, detecting, sealing and selling.

The method comprises the following specific steps:

firstly, 1000kg of harvested fresh leaf eating grass is cleaned by clear water and is put on an automatic vegetable cutter to be evenly cut into thin threads with the width of 0.36-60mm or smashed into 10-60 meshes of fragments by a beater.

Secondly, adding 500L of 300-5000L of tap water into a 2000-5000L jacket enamel reaction kettle or a 2000-5000L jacket 316 stainless steel reaction kettle which is stirred by steam or heat transfer oil or electric heating and is stirred at 2-3 r/min, and heating to 50-97 ℃.

Thirdly, adding 1000kg of chopped or smashed fresh leaf eating grass into the reaction kettle, stirring at the rotating speed of 10-15 r/min, keeping the temperature of the liquid mixture of the leaf eating grass fragments and water in the reaction kettle raised, keeping the temperature at any temperature of 50-97 ℃, stirring for 5-20 minutes, sampling, observing the color of the leaf eating grass fragments in the reaction kettle to become dark and soften.

Opening a bottom valve of the reaction kettle, pumping and discharging by using a 316 stainless steel pump with the inlet and outlet caliber of DN50-100 and the lift of 25-100 meters, pumping the discharged material, pressurizing the softened fragments of the leaf-eating grass into a plate-and-frame filter press with 10 tons/day, keeping the average surface pressure of the plate-and-frame filter press at 0.6-0.9MPa, and continuously performing filter pressing for 3-12 hours to stop filter pressing.

Collecting all press-filtered effluent of plate-and-frame filter press, pumping into another 8-12m stainless steel self-priming pump with caliber DN50-100 and lift of 50-100 m³The enamel jacketed reaction kettle or 316 stainless steel jacketed reaction kettle with stirring is started, the stirrer is started to stir at the rotating speed of 10-20 r/min, the liquid in the reaction kettle is kept, and the jacket circulating water is used for cooling until the leaf eating grass protein liquid in the reaction kettle reaches the normal temperature.

Dropping 10-36 wt% of food-grade NaOH solution into the protein solution filtered in the reaction kettle under the condition of continuous stirring until the pH value of the mixed solution filtered in the reaction kettle is 8-9, stopping dropping the NaOH solution, dropping 10-50% of H into the mixed solution filtered in the reaction kettle under the condition of continuous stirring₂O₂Solution until batchwiseSampling and observing, wherein the pressure filtration mixed liquid in the reaction kettle is changed from light green to colorless and transparent, and the dropwise addition of H is stopped₂O₂Obtaining the decolorized leaf-eating grass vegetable protein preparation solution.

The prepared leaf-eating grass plant protein preparation liquid is added into a feed inlet of a high-speed centrifugal spray dryer with the evaporation processing capacity of 500 plus materials of 1000 kg/h by a metering pump, the inlet air temperature of the preheated air heated by steam, natural gas burning or electric heating of the spray dryer is controlled to be 160 plus materials of 120 plus materials, and continuous feeding and discharging are carried out.

Packing the finished plant protein product of the leafy grass with the water content of 8-15 percent by a woven bag or a kraft paper bag lined with a polyethylene plastic bag according to 25-100 kg/bag from a discharge port of a spray dryer, inspecting, packaging, detecting, sealing and selling.

Ninthly, absorbing condensate water absorbed from hot air convection of tail gas of the spray dryer as backup liquid and storing the condensate water at 1000m³316 stainless steel tank, instead of tap water, for recycling.

And (c) discharging filter-pressed defoliating grass residues from the plate-and-frame filter press at the salt (r) according to the standard requirements of the nation or enterprises on animal feeds and according to various animals: adding fresh leaf-eating grass with the mass percentage of 50% of wet substances of leaf-eating grass residues into the leaf-eating grass residues according to the requirement of the nutrient mixing proportion of all nutrient elements required by pigs, cows, horses, sheep, chickens, ducks, geese, rabbits, dogs, cats, birds, crabs in the mixed feed, adding corn flour with the mass percentage of 8-20%, wheat bran with the mass percentage of 3-15%, rice bran with the mass percentage of 3-8%, calcium hydrophosphate with the feed grade of 1-2%, cottonseed cake powder or rapeseed cake powder with the feed grade of 0.3-100 meshes, salt with the feed grade of 0.3-0.6%, diatomite with the feed grade of 0.2-0.5%, vitamin B1 with the feed grade of 20-180ppm, B2, B12, VC, A and D, adding feed grade ferrous sulfate of 0.1-0.2ppm, adding crushed corn residues or non-polluted corn straws with the mass percentage of 10-10 meshes into the leaf-eating grass residues according to the mass ratio of 1: 10 The rice straw or the barren grass straw is uniformly mixed in a feed mixer to form the leaf eating grass tailing protein premix.

Adding 1000kg of the grass-eating tailing protein premix into 5-10kg of commercially available active yeast powder according to the proportion of 1000kg of the grass-eating tailing protein premix and using a 316 stainless steel screw mixer, stirring and mixing for 10-20 minutes, simultaneously dripping tail gas of a spray dryer to absorb circulating water to adjust the humidity of the material, keeping the total water content of the grass-eating tailing protein premix, and keeping the grass-eating tailing protein premix to be held into a ball without dripping water by hands.

Placing the grass-eating tailing protein premix into a factory, placing the grass-eating tailing protein premix on the ground using granite building blocks, covering the grass-eating tailing protein premix with a nontoxic polyethylene plastic film for sealing and heat preservation, naturally heating the grass-eating tailing protein premix to 30-40 ℃, carrying out heat preservation and fermentation, monitoring the temperature in real time, turning over and cooling when the temperature reaches 45 ℃, keeping the constant temperature of a material pile between 38-40 ℃, continuously fermenting for 1-3 days, and stopping fermentation to obtain the grass-eating tailing protein feed.

Naturally cooling the fermented leaf-eating grass tailing protein premix to room temperature, directly heating to 105 ℃ for 3 minutes, feeding animals such as pigs, cows, horses, sheep, rabbits, chickens, ducks and geese after cooling or drying and packaging at 120 ℃ by using a drum dryer to obtain the dry leaf-eating grass crude protein feed.

And (3) packing the fermented and dried crude leaf-eating grass protein feed finished product by using a lining polyethylene plastic bag and an outer packaging woven bag or kraft paper bag according to the weight of 25-100kg per bag, detecting, sealing and selling.

Detailed Description

Implementation mode one

Extracting plant protein from the leaf-eating grass and manufacturing the crude protein feed of the leaf-eating grass, and the method comprises the following steps: harvesting 1000kg of leaf eating grass after mature growth, cleaning the grass with clear water, uniformly cutting the grass into 2-100mm thin wires on an automatic vegetable cutter or breaking the grass into 10-300 mesh fragments by a beater, and adding the fragments into a 1500-plus 3000L reaction kettle with a 1500-plus 2000L jacket made of enamel or 316 stainless steel; heating water in a reaction kettle to 95-100 ℃ in advance by using steam or a jacket, starting a stirrer of the reaction kettle to stir at the rotating speed of 10-20 r/min, keeping the temperature of the liquid mixture of the leaf eating grass fragments in the reaction kettle raised and constant at the temperature of 95-100 ℃ under the condition of continuous heating, stirring and mixing for 2-10 minutes, sampling and observing the softening and enzyme deactivation of the leaf eating grass fragments in the reaction kettle; opening a valve from the bottom of the reaction kettle, and pumping and discharging by using a 316 stainless steel pump with the inlet and outlet diameter of DN50-100 and the lift of 100 meters; pressurizing the discharged material and pumping into a plate-and-frame filter press, and continuously performing filter pressing for 2-8 hours under the condition of keeping the average pressure of the plate-and-frame filter press at 0.6-0.8 MPa; collecting all the press-filtered water of plate-and-frame filter press, pumping into another 2-6m stainless steel self-sucking pump with diameter DN50-100 and 316 lift of 50 m³Starting a stirrer in the enamel jacket reaction kettle or the 316 stainless steel jacket reaction kettle, stirring at the rotating speed of 10-20 r/min, keeping the liquid in the reaction kettle, and cooling to normal temperature by using jacket water; and (3) pressing and filtering the mixed solution in the reaction kettle under the condition of continuous stirring, and selecting the following oxidant: adding 0.3-30% of food-grade sodium percarbonate aqueous solution or 0.5-10% of sodium peroxide aqueous solution or 0.1-1000ppm of chlorine dioxide aqueous solution in percentage by mass until decolorization, continuously stirring, sampling in batches, observing, filtering the mixed solution in the reaction kettle until the color of the mixed solution is changed from light color to colorless and transparent, and stopping adding the oxidant solution to obtain the decolorized leaf-eating grass vegetable protein preparation solution; (1) adding 1-9kg of lactobacillus powder serving as a food leaven into 100kg of normal-temperature tail gas absorption circulating water of a spray dryer, adding 100kg of liquid fresh milk, stirring, dissolving and mixing uniformly, and performing closed heat preservation fermentation at 25-40 ℃ for 1-5 days to obtain lactobacillus mother liquor; heating the decolorized leaf-eating grass vegetable protein preparation solution to 35-40 deg.C, adding commercially available 9kg leaf-eating grass protein preparation solution 1000kgAdding 300kg of fresh liquid milk according to the proportion of the active lactobacillus mother liquor, stirring for 1-2 minutes at the rotating speed of 3-10 revolutions per hour, adding all prepared lactobacillus mother liquor, uniformly stirring, keeping the temperature of 35-40 ℃ for closed fermentation for 2 days, filling and packaging according to the conditions of 0.3 liter/tin, 500 ml/tin, 1000 mg/tin, 2.51/tin, 5L/bucket, 10L/bucket, 25L/bucket, 100L/bucket and 200L/bucket to obtain the leaf-eating grass active milk protein milk, or carrying out gamma irradiation on the filling and packaging or sterilizing at 105 ℃ for 5 minutes to obtain the leaf-eating grass plant protein milk beverage, detecting and selling; (2) the prepared leaf-eating grass plant protein preparation liquid, the leaf-eating grass active milk protein milk and the leaf-eating grass plant protein milk beverage are respectively added into a high-speed centrifugal spray dryer with the evaporation processing capacity of 300 plus materials of 1000 kg/h by using a metering pump, the inlet air temperature of preheated air heated by steam or electricity of the spray dryer is controlled to be 160 ℃ plus materials, and the materials are continuously fed and discharged; the condensed water absorbed by the hot air tail gas of the spray dryer is used as a backup liquid and stored at 100-500m³The 316 stainless steel tank replaces tap water hot water for recycling; controlling the water content of the tea leaves discharged from a discharge port of the spray dryer to be 6-15%, and respectively obtaining finished products of the leaf-eating plant milk protein powder, the leaf-eating plant protein milk and the leaf-eating plant protein solid tea beverage; packaging finished products of the leaf eating plant protein powder food with canned or food grade polyethylene plastic bags or woven bags or kraft paper bags lined with plastic bags according to a ratio of 250 g/can, 250 g/bag, 500 g/bag, or 1kg, 2kg, 5kg, 10kg and 25-100 kg/bag, packaging, inspecting, sealing and selling; (3) the method comprises the following steps of (1) discharging the filter-pressed leaf eating grass residues from a plate-frame filter press according to the mass percentage of 100kg of the leaf eating grass residues, 10-20kg of corn flour, 2-8kg of bran and 1-2kg of other mineral calcium carbonate powder and 1% of salt, vitamins and trace elements serving as nutritional additives to various animals: the compound feed requirements of all nutrient elements required by users in the feed markets of pigs, cows, horses, sheep, chickens, ducks, geese, rabbits, dogs, cats, birds, pigeons, fishes, shrimps and crabs are added into the filter-pressed residue of the leaf-eating grass according to the national or enterprise standard requirements of the pre-designed compound proportion and are uniformly mixed by a mixer to form the protein fermentation premix of the tail residue of the leaf-eating grass; 1000kg of leaf-eating grass tailing protein premixAdding commercially available active lactobacillus powder with the mass of 1-20kg, adding 1-20kg of the commercially available active lactobacillus powder into 50-100kg of tail gas absorption circulating water in advance, adding 10-80kg of fresh milk, stirring, dissolving and mixing uniformly, performing closed heat preservation fermentation for 1-5 days at 25-40 ℃ in a 500L jacket enamel reaction kettle, then adding 1000kg of grass-eating tailing protein premix into a 316 stainless steel spiral knife mixer, starting up the mixer for mixing for 10-20 minutes, simultaneously dripping tail gas absorption circulating water or tap water, keeping the total water content of the grass-eating tailing protein premix, and kneading the grass-eating tailing protein premix into a cluster without dripping water by hands; placing the grass-eating tailing protein premix into a factory building, wherein the ground and the periphery of the factory building are protected by colorless epoxy terraces or are paved into a heat-preservation fermentation bin or a corrosion cement pool of the ground by granite, covering food-grade polyethylene plastic films on the grass-eating tailing protein premix, pressing edges around the food-grade polyethylene plastic films, sealing and preserving heat, naturally raising the temperature of a pile to 25-39 ℃, monitoring the temperature in real time, turning the pile to reduce the temperature when the temperature exceeds 43 ℃, keeping the constant temperature of the pile at 25-39 ℃, continuously fermenting for 1-3 days, and stopping fermentation; discharging the fermented grass-eating tailing protein premix to obtain a grass-eating crude protein fermented feed, and packaging and selling the grass-eating crude protein fermented feed; or drying in a drum dryer at 80-105 deg.C, packaging the fermented and dried feed product with polyethylene plastic bag, woven bag or kraft paper bag, and inspecting, sealing, and selling at 5 kg/bag, 10 kg/bag, 20 kg/bag, 25 kg/bag, 50 kg/bag, and 100 kg/bag. Packing the fermented and dried crude protein feed product of the leafy grasses by using a lining polyethylene plastic bag and an outer packing woven bag or a kraft paper bag according to 25-100kg per bag, detecting, sealing and selling; the leaf-eating plant protein milk beverage is recommended to be sold at a price of 10-20 yuan/kg; the leaf-eating plant milk protein powder is recommended to be sold at 40-50 yuan/kg; the leaf-eating plant protein milk is recommended to be sold at a price of 60-90 yuan/kg; the sale price of the crude protein feed product of the leaf-eating grass is suggested to be 9000-.

Second embodiment

Extracting plant protein from EYECAO and preparing crude protein fodder from EYECAOThe mass of the harvested fresh leaf eating grass is 1000kg-10000 kg; preparing a preparation solution of active lactobacillus seeds in advance: dissolving commercially available active lactobacillus powder 1-00kg in fresh milk 20-100kg in advance, adding into tap water 150-500kg, adding edible sodium chloride powder 100-300ppm, adding edible white sugar 10-30kg, stirring uniformly in a closed heat-insulating jacket enamel reaction kettle at 25-43 ℃, and fermenting for 1-3 days in advance; prepared 300-1000m²In the first warehouse, the factory building is required to be rainproof, the minimum effective area is 10m wide by 16m long, and a cement floor is arranged at the height of 3-10 m; laying 1.0-1.5mm thick clean 10m wide x 20m long polyethylene plastic paper on the cement terrace in advance; spraying and stirring the freshly harvested fresh leaf-eating grass with the mass of 1000kg-10000kg by using prepared active lactobacillus preparation liquid; according to the weight ratio of 1000kg of fresh leaf eating grass and the ratio of 100: 1, 10kg of edible diatomite by weight is mixed and added: the calcium carbonate powder with the mass of 15kg and 500 meshes is uniformly stirred to form a leaf eating grass fermentation mixture; placing the mixture of the lactobacillus, the salt, the diatomite and the calcium carbonate on a cement terrace: the mixture is piled up in a trapezoidal shape on a prepared plastic paper with the width of 10m and the length of 20m, and the mixture is piled on the trapezoidal leaf-eating grass mixture, and is sealed and covered with a piece of plastic paper, the four sides of the plastic paper are compacted, and air leakage cannot occur. Naturally heating the leaf-eating grass mixture pile for fermentation, keeping the pile temperature at 25-42 ℃ for fermentation for 1-2 days in summer, and keeping the pile temperature at 25 ℃ for fermentation for 6-15 days in winter; when the temperature of the naturally fermented leaf-eating grass mixed material pile exceeds 42 ℃, turning and cooling, continuously stacking for natural heat preservation fermentation for 1-2 days, stopping fermentation, discharging the fermented leaf-eating grass mixed material pile to obtain leaf-eating grass fermented feed raw materials, directly feeding the leaf-eating grass fermented feed raw materials to pigs, cows, horses, sheep, chickens, ducks, geese, rabbits and the like according to the requirements, or serving as compound feed or crushing the leaf-eating grass fermented feed raw materials, drying and packaging at 105-120 ℃, and selling; the suggested sale price is 5000-; crushing the raw materials of the leaf-eating grass fermented feed, and continuously mixing the raw materials with other crop straws, vegetable straws, feed-grade grain plants and the like which do not contain pesticides or heavy metals and organic matter pollution according to the required proportion:mixing, stacking, preserving heat, fermenting, turning over and controlling temperature to sequentially ferment to obtain the straw fermented feed for the leaf-eating grass farmer, directly feeding the straw fermented feed for animals after crushing or drying and packaging the straw fermented feed for sale at 105-120 ℃, and the suggested sale price is 3800-. The fermentation precautions are as follows: the strains are uniformly mixed, the fermentation pile is sealed and insulated, and turned over in time, the high-temperature burning pile exceeding 45 ℃ is prevented, and the problems that the distribution of acid-wetting bacteria sprayed on the leaf-eating grass mixture is uneven, and the leaf-eating grass mixture is yellow, mildewed and rotten are prevented. The liquid flowing out of the leaf-eating grass mixed material pile is recycled and placed in a plastic barrel to be used as the preparation liquid of the next lactic acid bacteria seed to replace active lactic acid bacteria and milk, and water is added for recycling, so that the production cost is reduced.

Third embodiment

Extracting plant protein from the lysimachia foenum-graecum and preparing a lysimachia foenum-graecum crude protein feed, and preparing the nutrient health-care lysimachia foenum-graecum golden wine of the lysimachia foenum-graecum root flavone and the plant protein by utilizing the lysimachia foenum-graecum root and adding Chinese medicinal materials: cleaning the roots of the leafy herbs which grow for 3 to 10 years, drying in the sun, drying in the shade or drying by infrared rays, matching plant proteins extracted from the leafy herbs with northeast ginseng, astragalus, kudzuvine root, tuber fleeceflower root, mantis egg-case, corn silk, roselle, pseudo-ginseng, green walnut, red date, hornet nest, eclipta alba, raw rehmannia root, spina gleditsiae, sophora fruit, cistanche, Japanese ardisia, pilose antler, cicada flower, poria cocos, angelica, prepared rehmannia root, dwarf lilyturf tuber, Japanese milkwort herb, dried orange peel, medlar, dogwood fruit, Huichuan lovage rhizome, divaricate saposhnikovia root, tortoise plastron glue, Chinese magnoliavine fruit, notopterygium root, cynomorium songaricum, morium morinda root, south dodder seed, motherwort herb, parched eucommia bark leaf, parched leafy herb, bud, dipsacus root, sharpleaf galangal fruit, flatstem, semen astragali, tuber onion seed, actinolite, hippocampus, oyster, glossy privet fruit, monkshood fruit, placenta hominis, Gecko, semen Juglandis, testis et penis Callorhini, Inonotus obliquus, Kadsura coccinea, caulis Marsdeniae Tenacissimae, herba Cynanchi Stauntonii, Ginseng radix, Angora carbene bark, and flos Albizziae by mixing with 55-65% (v/v) Kaoliang spirit and soaking; mixing six to ten kinds of the traditional Chinese medicinal materials according to the mass ratio of 1: 1 or 1: 1, taking dried or sun-dried traditional Chinese medicinal materials, adding 60-300kg of the mixture according to the mass ratio into an enamel reaction kettle which is placed in advance in a clean 5000-plus-12000L, adding 12000-plus-19000L of 52-55 DEG pure grain brewed liquor, starting a stirrer at 8-20 r/min for stirring uniformly, stopping stirring, soaking the traditional Chinese medicinal materials for one to six months after standing, using 55-DEG base liquor of the sorghum liquor, adjusting the sorghum liquor in the reaction kettle to be 52-53 DEG, filtering out traditional Chinese medicine residues, placing the traditional Chinese medicine residues in a wine jar for natural precipitation for 30 days, taking supernatant, filling 600ml of each 500-plus-liquor into a wine bottle, adding one herbivore root into each wine bottle, covering the wine bottle, and detecting for sale. The health care grass golden wine containing the plant protein extracted from the herbivorous herbs is compiled by product application units. The recommended grass meal golden wine is 500 ml/bottle, the health care value is different according to the variety of the added traditional Chinese medicinal materials, and the retail price of 500 ml/bottle is 390-; soaking all the Chinese medicinal residues after soaking in 55 ° Kaoliang spirit for 30 days, filtering to obtain Chinese medicinal residues, and mixing with the second medicated liquor as the next medicated liquor concentration regulator; the residual traditional Chinese medicine dregs are prepared by the following steps of taking 30 mass percent of the traditional Chinese medicine dregs, adding 40 mass percent of fresh leaf eating grass, adding 10 mass percent of corn starch and adding 18 mass percent of millet according to the mass percent of the dried materials of the traditional Chinese medicine dregs at 100 ℃, uniformly mixing, adding 2 mass percent of commercial yeast powder, adding proper amount of water, keeping the water content of the whole materials, holding the materials by hands to form a ball without dripping water, sealing the ball in a fermentation ceramic vat, fermenting the ball for 1 to 6 hours at 25 to 43 ℃, sterilizing the ball by gamma ray irradiation, packaging and sealing the ball by 10 to 50 grams per bag by using a polyethylene plastic bag, selling the ball as a bait for fishing enthusiasts or as fish feed and dog feed, adding partial calcium magnesium mineral substances and zinc trace elements, namely salt vitamin B1, B2 and E, and also being used as a compound feed for breeding pigs or being prepared into pellet feed for precious animals, packaging to be sold as pet nutritional feed; the sale price of the pet feed is suggested to be 12000-18000 yuan/ton (tax price).

Claims

1. Extracting plant protein from leaf-eating grass and preparing crude protein feed of leaf-eating grass, and harvesting 1000kg of fresh food according to the stepsCleaning the leaves and grass with clear water, uniformly cutting the leaves and grass into thin threads with the width of 0.36-60mm on an automatic vegetable cutter or smashing the leaves and grass into 10-60 meshes of fragments by a beating machine; secondly, adding 500L of 300-5000L of tap water in advance into a 2000-5000L jacket enamel reaction kettle or a 2000-5000L jacket 316 stainless steel reaction kettle which is stirred by steam or heat transfer oil or electric heating and is stirred at 2-3 r/min, and heating to 50-97 ℃; thirdly, adding cut or smashed fresh leaf eating grass with the mass of 1000kg, stirring at the rotating speed of 10-15 r/min, keeping the temperature of the liquid mixture of the leaf eating grass fragments and water in the reaction kettle raised, keeping the temperature at any temperature of 50-97 ℃, stirring for 5-20 minutes, sampling, observing the color of the leaf eating grass fragments in the reaction kettle to become dark and soften; opening a valve at the bottom of the reaction kettle, pumping and discharging by using a 316 stainless steel pump with the inlet and outlet caliber of DN50-100 and the lift of 25-100 meters, pumping the discharged material, pressurizing and pumping the softened fragments of the leaf eating grass into a plate-and-frame filter press with the weight of 10 tons/day, keeping the average surface pressure of the plate-and-frame filter press at 0.6-0.9MPa, continuously performing filter pressing for 3-12 hours and stopping the filter pressing; collecting all press-filtered effluent of plate-and-frame filter press, pumping into another 8-12m stainless steel self-priming pump with caliber DN50-100 and lift of 50-100 m³Starting a stirrer in the enamel jacket reaction kettle or the 316 stainless steel jacket reaction kettle with the stirrer, stirring at the rotating speed of 10-20 r/min, keeping the liquid in the reaction kettle, and cooling by circulating water through the jacket until the leaf eating grass protein liquid in the reaction kettle reaches the normal temperature; dropping 10-36 wt% of food-grade NaOH solution into the protein solution filtered in the reaction kettle under the condition of continuous stirring until the pH value of the mixed solution filtered in the reaction kettle is 8-9, stopping dropping the NaOH solution, dropping 10-50% of H into the mixed solution filtered in the reaction kettle under the condition of continuous stirring₂O₂The solution is sampled and observed in batches, the pressure filtration mixed solution in the reaction kettle is changed from light green to colorless and transparent, and the dropwise addition of H is stopped₂O₂Obtaining a decolorized leaf-eating grass vegetable protein preparation solution; seventhly, the prepared leaf grass vegetable protein preparation liquid is added into a feed inlet of a high-speed centrifugal spray dryer with the evaporation processing capacity of 500 plus one 1000 kg/h by a metering pump, and the steam heating, the natural gas burning or the electric heating of the spray dryer are controlled to be performedThe inlet air temperature of the hot air is 160 ℃ below 120 ℃, and the materials are continuously fed and discharged; packing the finished plant protein product of the leafy grass with the water content of 8-15 percent by a woven bag or a kraft paper bag lined with a polyethylene plastic bag according to 25-100 kg/bag, inspecting, packaging, detecting, sealing and selling; ninthly, absorbing condensate water absorbed from hot air convection of tail gas of the spray dryer as backup liquid and storing the condensate water at 1000m³The 316 stainless steel tank replaces tap water hot water for recycling; and (c) discharging filter-pressed defoliating grass residues from the plate-and-frame filter press at the salt (r) according to the standard requirements of the nation or enterprises on animal feeds and according to various animals: adding fresh leaf-eating grass with the mass percentage of 50% of wet substances of leaf-eating grass residues into the leaf-eating grass residues according to the requirement of the nutrient mixing proportion of all nutrient elements required by pigs, cows, horses, sheep, chickens, ducks, geese, rabbits, dogs, cats, birds, crabs in the mixed feed, adding corn flour with the mass percentage of 8-20%, wheat bran with the mass percentage of 3-15%, rice bran with the mass percentage of 3-8%, calcium hydrophosphate with the feed grade of 1-2%, cottonseed cake powder or rapeseed cake powder with the feed grade of 0.3-100 meshes, salt with the feed grade of 0.3-0.6%, diatomite with the feed grade of 0.2-0.5%, vitamin B1 with the feed grade of 20-180ppm, B2, B12, VC, A and D, adding feed grade ferrous sulfate of 0.1-0.2ppm, adding crushed corn residues or non-polluted corn straws with the mass percentage of 10-10 meshes into the leaf-eating grass residues according to the mass ratio of 1: 10 Uniformly mixing rice straws or waste grass straws in a feed mixer to form a leaf eating grass tailing protein premix;

adding 1000kg of grass-eating tailing protein premix and 5-10kg of commercially available active yeast powder according to the proportion of 1000kg of grass-eating tailing protein premix and 316 stainless steel screw mixer, stirring and mixing for 10-20 minutes, simultaneously dripping tail gas of a spray dryer to absorb circulating water to adjust the humidity of the material, keeping the total water content of the grass-eating tailing protein premix, and keeping the grass-eating tailing protein premix to be held into a ball by handsNo water dripping;

placing the grass-eating tailing protein premix into a factory building, placing the grass-eating tailing protein premix on the ground using granite building blocks, covering a nontoxic polyethylene plastic film on the grass-eating tailing protein premix, sealing and insulating the grass-eating tailing protein premix, naturally heating the grass-eating tailing protein premix to 30-40 ℃, insulating and fermenting, monitoring the temperature in real time, turning over and cooling when the temperature reaches 45 ℃, keeping the constant temperature of a material pile between 38 and 40 ℃, continuously fermenting for 1-3 days, and stopping fermentation to obtain the grass-eating tailing protein feed;

naturally cooling the fermented leaf-eating grass tailing protein premix to room temperature, directly heating to 105 ℃ for 3 minutes, feeding animals such as pigs, cows, horses, sheep, rabbits, chickens, ducks and geese after cooling or drying and packaging at 120 ℃ by using a drum dryer to obtain dry leaf-eating grass crude protein feed;

according to 25-100kg per bag, packing the fermented and dried crude protein feed finished product of the leaf-eating grass by using a lining polyethylene plastic bag and an outer packaging woven bag or a kraft paper bag, detecting, sealing and selling;

the unit of application for extracting the plant protein from the leaf-eating grass is compiled into the leaf-eating grass plant protein SYC-906.

2. The method comprises the steps of washing 1000kg of fresh leaf-eating grass which is harvested with clear water, putting the fresh leaf-eating grass on an automatic vegetable cutter, uniformly cutting the fresh leaf-eating grass into thin threads with the width of 0.36-60mm or smashing the fresh leaf-eating grass into 10-60 meshes of fragments by a beater.

3.③ adding 1000kg of fresh chopped or smashed leaf eating grass, stirring at the rotating speed of 10-15 r/min, keeping the temperature of the liquid mixture of the leaf eating grass fragments and the water in the reaction kettle raised, stirring for 5-20 minutes at any constant temperature of 50-97 ℃, sampling, observing the color of the leaf eating grass fragments in the reaction kettle to become dark and soften.

4. Sixthly, according to claim 1, adding food-grade NaOH solution with the mass percentage of 10-36% into the protein solution filtered in the reaction kettle in a dropwise manner under the condition of continuous stirring until the pH value of the mixed solution filtered in the reaction kettle is 8-9, stopping adding the NaOH solution in a dropwise manner, adding 10-50% of H into the mixed solution filtered in the reaction kettle in a dropwise manner under the condition of continuous stirring₂O₂The solution is sampled and observed in batches, the pressure filtration mixed solution in the reaction kettle is changed from light green to colorless and transparent, and the dropwise addition of H is stopped₂O₂Obtaining the decolorized leaf-eating grass vegetable protein preparation solution.

5. The method as claimed in claim 1, wherein the prepared leaf-eating grass plant protein preparation liquid is added into the feed inlet of a high-speed centrifugal spray dryer with the evaporation processing capacity of 500-1000 kg/h by a metering pump, and the inlet air temperature of the preheated air heated by steam, natural gas burning or electric heating of the spray dryer is controlled at 160-120 ℃, and the continuous feeding and discharging are carried out.

6. According to claim 1, the filter-pressed residue of herbivore discharged from plate-and-frame filter press is processed according to national or enterprise standard for animal feed, according to various animals: adding fresh leaf-eating grass with the mass percentage of 50% of wet substances of leaf-eating grass residues into the leaf-eating grass residues according to the requirement of the nutrient mixing proportion of all nutrient elements required by pigs, cows, horses, sheep, chickens, ducks, geese, rabbits, dogs, cats, birds, crabs in the mixed feed, adding corn flour with the mass percentage of 8-20%, wheat bran with the mass percentage of 3-15%, rice bran with the mass percentage of 3-8%, calcium hydrophosphate with the feed grade of 1-2%, cottonseed cake powder or rapeseed cake powder with the feed grade of 0.3-100 meshes, salt with the feed grade of 0.3-0.6%, diatomite with the feed grade of 0.2-0.5%, vitamin B1 with the feed grade of 20-180ppm, B2, B12, VC, A and D, adding feed grade ferrous sulfate of 0.1-0.2ppm, adding crushed corn residues or non-polluted corn straws with the mass percentage of 10-10 meshes into the leaf-eating grass residues according to the mass ratio of 1: 10 The rice straw or the barren grass straw is uniformly mixed in a feed mixer to form the leaf eating grass tailing protein premix.

7. According to claim 1.

8. According to claim 1.

9. According to claim 1.

Naturally cooling fermented grass-eating tailing protein premix to room temperature, directly heating to 105 deg.C for 3 min, cooling, and feeding to animals such as pig, cattle, horse, sheep, rabbit, chicken, duck, goose, etcDrying and packaging the coarse protein feed by a roller dryer at 120 ℃ to obtain the dry coarse protein feed of the leaf-eating grass.

10. According to claim 1.