CN108633626A - The method for preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding - Google Patents
The method for preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
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Abstract
A method of White mushroom cultivation base being prepared by primary raw material of cattle pen bedding and padding, preparation process is：（1）50~60 parts of cattle pen bedding and padding, 15~25 parts of Chinese mesona herb slag, 5~15 parts of agstone, 5~10 parts of vermiculite power, 3~8 parts of blackstrap, 1~2 part of EM bacterium are taken by weight；（2）Cattle pen bedding and padding, Chinese mesona herb slag, agstone, vermiculite power and EM bacterium are uniformly mixed and obtain premix；（3）Premix is put into after fermenting 25~30 days in tunnel fermentation kiln, adds blackstrap and EM bacterium are uniformly mixed, ferment 3~5 days, fermentation temperature is controlled at 28 DEG C~30 DEG C；（4）By the premix after fermentation after pasteurization 10~15 hours, cultivation base is obtained；Sterilisation temp is 60 DEG C~65 DEG C.White mushroom yield height, the meat that the cultivation base that the method for the present invention obtains is cultivated are abundant, delicious flavour.
Description
Technical field
It is specially a kind of to replace obtained useless cattle pen bedding and padding as primary raw material system the invention belongs to fungus growing technique
The method of standby White mushroom cultivation base.
Background technology
White mushroom（Agaricusbisporus）, mycelia silvery white is fast in the speed of growth, is not easy to tie bacterium quilt, fructification
More Dan Sheng, circle just, white, without scale, cap is thick, is not easy parachute-opening, thick relatively straight short in stem, and bacterial context white organizes solid, stem
On have half membranaceous collarium, spore silver brown.White mushroom category straw rotting fungus, middle low temperature properties mushroom class, China wheat straw, wheat straw are abundant, weather
Compare the growth of suitable White mushroom, has the potentiality that grow a lot.Many countries have cultivation, wherein China's total yield to account for second,
Canned mushroom account for the first in the quantum of international trade.The bacterial context fertilizer of White mushroom is tender, and contains more mannose, trehalose and various
Amino acids substance, so delicious flavour, full of nutrition, according to surveying and determination per the dry mushrooms of 100g in 36-40g containing protein, fat 3.6g,
Carbohydrate 31.2g, phosphorus 718mg, iron 188.5mg, calcium 131mg, ash content 14.2mg, crude fibre 6g additionally contain dimension life
Plain B1, B2, Vc, niacin etc., since its nutrition is higher than general vegetables, so there is the title of " Vegetable meat ".
White mushroom is one of most common edible bacterium, fleshy hypertrophy.It is primary in Europe and North America, the mankind are at least from ancient times
Just start to eat from Greece's epoch, artificial cultivation then about starts from the ground such as the France of the 17th century, now then each in the world extensively
It cultivates on ground.Cultivation matrix is the place for the various nutriments that edible mushroom depends on for existence.Using kind and growing environment condition
In the case of identical, formula difference different using matrix, the processing mode of matrix are different, the yield of edible mushroom, quality, flavor
Deng all difference.Open source literature also reports the cultivation matrix of some White mushrooms, such as：
1, Chinese patent：Using useless padding for pig sty as the White mushroom culture material of primary raw material, application number：201711152428.8 Shen
Please day 2017.11.19, applicant：The Guangxi bio tech ltd Ren Tai, address：530306 Guangxi Zhuang Autonomous Region Nanning
Mushroom factory of the city Heng County towns the Yun Biao villages Chao Nan, inventor：Yang Wei, Wei Rongjie, Wang Yuanneng, Wu Feng, abstract：One kind is with padding for pig sty
The White mushroom culture material of primary raw material, it is prepared from the following raw materials in parts by weight：Useless 45-60 parts of padding for pig sty；Chinese mesona herb slag
20-40 parts；15-25 parts of manioc waste；10-20 parts of straw particle；5-10 parts of ramulus mori particle；5-8 parts of corn ear slag particle；Bagasse
3-5 parts of particle；The Chinese mesona herb slag is the Chinese mesona herb slag that bean jelly processing factory is obtained by filtration using Chinese mesona herb processing bean jelly.This is double
Spore mushroom culture material use pig farm be discharged useless padding for pig sty White mushroom culture material is prepared for primary raw material, belong to change give up into
Precious project does not discharge waste residue externally, environmentally protective；The White mushroom culture material plantation White mushroom fruiting being prepared is early, can adopt mushroom
Time is long, and can reduce White mushroom cultivation cost, improves White mushroom and cultivates economic benefit.
2, Chinese patent：A kind of selenium-enriched agaricus bisporus cultivation matrix, application number：201710618811.1 the applying date：
2017.07.26 applicant：The Pujiang County bio tech ltd Mei Ze, address：322200 Jinhua, Zhejiang Province city Pujiang County are too
White road 23 Stall is exposed to the north, inventor：Xie Yuxin, abstract：The invention discloses a kind of selenium-enriched agaricus bisporus cultivation matrix, the matrix
Ingredient and its parts by weight be：22-28 parts of corn stalk, 18-23 parts of wheat straw, 20-26 parts of cow dung, 15-18 parts of chicken manure, Chinese medicine slag 3-
5 parts, 6-8 parts of corn flour, 0.01-0.015 parts of sodium selenite, yeast selenium 0.005-0.008,2-4 parts of urea, precipitated calcium carbonate 1-
2 parts, 0.7-1 parts of calcium superphosphate, 1-2 parts of land plaster, 1-2 parts of quick lime, 0.013-0.016 parts of fungicide, 1-2 parts of leavening.
It has the beneficial effect that：Selenium source is uniformly distributed in cultivation matrix of the present invention, and good permeability, retention ability is strong, and cultivation matrix is decomposed fully,
It is easy to be absorbed and utilized by White mushroom, the cultivation period of White mushroom is made to shorten, turns out organic selenium content height, White mushroom in White mushroom
Yield and biological efficiency it is higher, be suitble to common peasant household's large-scale promotion.
3, Chinese patent：A kind of White mushroom culture substrate, application number：201310292871.1 the applying date：
2013.07.12 applicant：Shandong Fanglv Agricultural Science and Technology Co., Ltd., address：256200 Shandong Province Accessories during Binzhou Zouping County's economy
Development zone, inventor：Kou Yufang, Zhang Li, Han Chao, Zhang Fabo, abstract：The invention belongs to fungus growing technique fields, specifically
It is related to a kind of White mushroom culture substrate.The White mushroom culture substrate of the present invention, is made of the raw material of following parts by weight：Chicken manure
19-27 parts, 0.30-0.50 parts of lime, 28-36 parts of stalk, 1.2-2.0 parts of gypsum, 0.15-0.35 parts of urea, cake fertilizer 0.70-
0.90 part, 0.06-0.14 parts of calcium superphosphate.Double spores are cultivated using the matrix of the fermentation process preparation of White mushroom matrix of the present invention
Mushroom, every square metre of production mushroom 22kg, is higher by 1-2kg wherein level-one mushrooms than other matrix formulations and accounts for 90%, single mushroom heavier-weight, mushroom type
Roundness is good, and easily fortune easily storage has, and can be stored 4-5 days in 0 DEG C -4 DEG C of freezer, non-discolouring, does not play scale, compares in same market
Other fresh mushrooms price per jin is higher by 0.3 yuan, increases planting benefit.
4, Chinese patent：A kind of culture substrate of White mushroom, application number：201410529324.4 the applying date：
2014.10.09 applicant：Zunyi makes innovations Bacteria Co., Ltd., address：563000 Zunyi City, Guizhou Province Honghuagang District Shen Xi
The town villages great Wo, inventor：Zheng Mingfeng, abstract：The invention discloses a kind of culture substrates of White mushroom, it is by following parts by weight original
Material is made：52-58 parts of cotton seed hulls, 32-38 parts of sawdust, 18-24 parts of wheat skin, 4-10 parts of calcium carbonate, 5-11 parts of calcium superphosphate, urea
4-10 parts, 17-23 parts of cow dung.Proportioning by various raw materials and control, by the whole nutrient abundantization of entire compost,
The addition of vitamin can meet the growth demand of White mushroom to the full extent, can by basal culture medium matter cultivating agaricus bisporus
The yield and quality of White mushroom is improved, and cost is relatively low, it is easy to operate, it is easy to spread.
5, Chinese patent：A kind of White mushroom cultivation matrix and preparation method thereof, application number：201510549748.1 application
Day：2015.09.01 applicant：Nanning Sheng Qian Science and Technology Development Co., Ltd.s, address：530022 Guangxi Zhuang Autonomous Region
The Nanning City Qingxiu District roads Dong Ge 39, inventor：Wei Bo, abstract：The invention discloses a kind of White mushroom cultivation matrix and its systems
Preparation Method belongs to edible fungus culturing matrix technology field.The cultivation matrix is obtained by following components mixed preparing：Wheat straw, grass
Charcoal, wheat bran, rice bran, land plaster, pulverized limestone, calcium superphosphate, ammonium sulfate, potassium sulfate, fermentation of organic material；The fermentation of organic material is
Using feces of livestock and poultry, silkworm excrement, biogas residue, agricultural crop straw as fermentation raw material, add fermenting agent, stacking, spontaneous fermentation and obtain.This hair
It, can in the bright fermentation for using a variety of strains to be used for waste material recycling for the specific proportioning high efficiency composition strain of component
Fermentation time is shortened, fermentation efficiency is improved and animal wastes are resolved into the ability of innocuous substance, is eliminated in fermentation process
The stink of generation, and the content of organic matter after fermenting crops straws can be improved, the nutrient of the fertilizer produced after fermentation is improved, also
Powerful probiotics environment can be formed, the disease resistance of edible mushroom is improved.
6, Chinese patent：A kind of production method of White mushroom nutrient matrix, application number：201510330198.4 the applying date：
2015.06.16 applicant：Wang Jinsheng, address：236000 belt North Road Yongan lane, Fuyang City Yingquan District, Anhui Province 36, invention
People：Wang Jinsheng, blue sea of clouds, abstract：The present invention relates to a kind of production methods of White mushroom nutrient matrix, by drying sheep manure 12-
15 parts, 3-5 parts of Soybean Root, dry 10-13 parts of cow dung, 0.2-0.5 parts of zinc chloride, 1-3 parts of sucrose, 2-5 parts of lime, potassium pyrophosphate
4-6 parts, 1-3 parts of compositions of peanut meal, a kind of production method of White mushroom nutrient matrix provided by the invention, nutritional sufficiency, energy
Enough nutritional needs for fully meeting White mushroom, the White mushroom meat cultivated is abundant, and full of nutrition, taste is relative to generic media
The White mushroom cultivated is more delicious, also contains abundant zinc.
Invention content
The mesh of the present invention is to provide a kind of preparing White mushroom cultivation base to replace obtained useless cattle pen bedding and padding as primary raw material
Method, the White mushroom yield cultivated with the cultivation base is high, meat is abundant, delicious flavour.
To achieve the goals above, the technical proposal of the invention is realized in this way：
A method of White mushroom cultivation base being prepared by primary raw material of cattle pen bedding and padding, preparation process is：
（1）Take by weight 50~60 parts of cattle pen bedding and padding, 15~25 parts of Chinese mesona herb slag, 5~15 parts of agstone, vermiculite power 5~
10 parts, 3~8 parts of blackstrap, 1~2 part of EM bacterium；The agstone and vermiculite power is after using lime stone and vermiculite to crush respectively
Cross the flour that 80 mesh are obtained with upper screen cloth.Cattle pen bedding and padding are the useless bedding and padding replaced from cattle pen.
（2）Cattle pen bedding and padding, Chinese mesona herb slag, agstone, vermiculite power and EM bacterium are uniformly mixed and obtain premix.
（3）Premix is put into after fermenting 25~30 days in tunnel fermentation kiln, adds blackstrap and EM bacterium are uniformly mixed,
Fermentation 3~5 days, fermentation temperature are controlled at 28 DEG C~30 DEG C.
（4）By the premix after fermentation after pasteurization 10~15 hours, cultivation base is obtained；Sterilisation temp is 60 DEG C
~65 DEG C.After pasteurization, need to be cooled to 25 DEG C or less could be implanted into White mushroom kind for premix after fermentation.The double spores of implantation
Mushroom kind need to be in the moistening soil of culture material upper table cover 4-5cm after 15 days.Moistening soil is to utilize pond sludge, yellow soil and soddy soil
After smashing mixing, clear water is added to being sufficiently humidified so as to obtain the final product, the weight ratio of pond sludge, yellow soil and soddy soil is：3:2:（0.1-
0.5）.
The cattle pen bedding and padding include that the raw material of following parts by weight is made：It is 35~50 parts of the wooden chaff, 25~45 parts of rice husk, sweet
10~25 parts of bagasse, 5~15 parts of sugarcane marrow, 5~15 parts of fermentation material, 3~10 parts of forage waste residue, ox urinate 3~8 parts, the dry slag of cow dung 2~
3 parts；The forage waste residue, which is that cattle eat grass, expects remaining residue.
The fermentation material be add strain using culture medium, the stomach slag and gastric juice that is taken out from ox ruminant stomach, ox urine and ox
The fermentation material that argol slag culture obtains.
The culture medium, raw material include following parts by weight：15~20 parts of the wooden chaff, 10~15 parts of rice husk, eucalyptus branches and leaves
5~10 parts of particle, 5~15 parts of sugarcane marrow, 1~3 part of ferrous sulfate, 2~5 parts of urea；Eucalyptus branches and leaves particle is to utilize eucalyptus branches and leaves
It is ground into granule size and is less than 0.5 centimetre.
The strain includes the raw material of following parts by weight：2 parts of saccharomycete, 1 part of bacillus subtilis, 1 part of cellulase,
1 part of EM bacterium, 0.5 part of Rhodopseudomonas palustris, 0.5 part of candida utili, 0.1 part of lactobacillus plantarum.
The incubation of the fermentation material is to be uniformly mixed culture medium, strain, ox urine and the dry slag of cow dung, culture medium,
The weight ratio of strain, ox urine and the dry slag of cow dung is 100:（1~3）:30:20, after one week of stationary culture, addition is ruminated from ox
The stomach slag and gastric juice that are taken out in stomach are uniformly mixed, after standing 3~4 days to get；The stomach slag and gastric juice that are taken out from ox ruminant stomach with
The weight ratio of culture medium is（3~4）:100.
The preparation process of the bedding and padding of the vaccary cattle pen is：
（1）The wooden chaff, rice husk, bagasse, sugarcane marrow, fermentation material, forage waste residue, ox urine, the dry slag of cow dung are taken by above-mentioned parts by weight, and
By bagasse and forage waste residue be ground into granule size be less than 1 centimetre, it is spare；
（2）After mixing by the wooden chaff, rice husk, bagasse, sugarcane marrow, fermentation material and the dry slag of cow dung, the ox urine that half amount is added is mixed
It closes uniformly, is left to ferment 3~5 days, obtains fermentate；
（3）Forage waste residue is added in fermentate to be uniformly mixed, the ox urine for adding the other half amount is uniformly mixed, standing for fermentation 3
After it, until fermentate is dry and comfortable to obtain the final product.When the wooden chaff of appearance is hardened during cowboying, harrowed with rotary cultivator or tool loose；When bedding and padding are wet
It spends big when there is peculiar smell, is sprinkling upon on bedding and padding with fermentate, the bedding and padding per quadrature add 1~2 kilogram.
It is provided with multiple rows of blow vent on the tunnel fermentation kiln ground, blow vent can be passed through compressed air, periodically to pre-
Batch mixing carries out logical oxygen.The ferment ground of kiln of tunnel is hardening of cement ground, lays snorkel before hardened ground, when hardening there are
Compressed gas can be discharged from blow vent for blow vent, snorkel.The left side, the right side and the inside of tunnel fermentation kiln are partition wall, top surface
It is provided with the sunshade that keeps off the rain.Premix is deposited in ground by the White mushroom culture material of the present invention outward inside tunnel fermentation kiln
On, it covers on blow vent, the compressed gas in snorkel is discharged from blow vent, provides oxygen for premix and keeps premix
The temperature of fermentation.
The method for preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding of the present invention can also prepare oyster mushroom, chicken leg
Mushroom, needle mushroom and mushroom cultivation base.
Chinese mesona herb (scientific name:Mesona chinensis BenthIt is) labiate, the lower portion of the stem is thrown oneself on the ground, and top is upright,
Leaf is oval or ovate oblong, and apex is slightly blunt, and base portion, which tapers, shortens handle into, and there is small sawtooth at edge, and two sides, which has to dredge, becomes mildewed;It is born in
The leaf on inflorescence top is smaller, be in bracteal, it is oval to up-side down triangle, relatively spend it is short, base portion often band lavender, as a result when fall off.Always
Locusta is weak, spends small, and verticillate, calyx is small, mitriform, and 2 lips, upper lip 3 is split, lower lip full edge, as a result when or tubular, under it is curved, have vertical
Arteries and veins and horizontal wrinkle；Corolla pale red, upper lip is wealthy, full edge or fissure, lower lip oblong, recess；Stamen 4, filigree protrude；Gynoecium
1, style 2 is split；Floral disc expands on one side.Pyrene ellipse.Autumn Mo at florescence.It is distributed in TaiWan, China, Zhejiang, Jiangxi, Guangdong,
West Guangxi.Chinese mesona herb is a kind of important medical and edible dual purpose plant resource.Chinese mesona herb herb contains polysaccharide, relieves summer heat, is heat-clearing, cool
Blood, function of detoxification.Civil its stem of commonly using adds water to cook, then adds dilute starch that jelly (being commonly called as " bean jelly ") is made and eat, and is to relieve summer heat to quench one's thirst
Splendid food.The Chinese mesona herb slag that the present invention uses is that bean jelly processing factory processes bean jelly, that is, mistake after adding water to cook using Chinese mesona herb
Filter obtained Chinese mesona herb slag.Need to be dehydrated when the application is using Chinese mesona herb slag to water content could use for 50-70%.
The yeast (saccharomyce) it is common eucaryote recipient cell in gene cloning experiment, cultivate ferment
Female bacterium facilitates as culture Escherichia coli.The type of yeast cloning vector is also very much.Saccharomycete is this also with the presence of plasmid
The plasmid of 2pm long is known as 2um plasmids, about 6300bp.This plasmid be at least present in for some time in nucleus chromosome with
Outside, the shuttle that can be shuttled between bacterium and yeast cell can be built into using the plasmid in 2pm plasmids and Escherichia coli
Plasmid.Yeast is some unicellular fungis, not the unit of phylogeny classification.It is a kind of invisible small unicellular micro-
Sugar can be fermented into alcohol and carbon dioxide, be distributed in entire nature by biology, be a kind of typical amphimicrobe,
It can survive under aerobic and oxygen free condition, be natural fermented dose a kind of.
The bacillus subtilis, Classification system：Bacillussubtilis, it is one kind of bacillus.Withered grass
Bacillus has stronger protease, amylase and lipase active, can be active enzyme by internal activation of zymogen, may be used also
Other enzymes of secretion-series, such as pectase, dextranase, cellulase can help to decompose non-starch polysaccharide etc. in plant feed
Substance improves efficiency of feed utilization, and acid intestinal environment can stimulate the intestines peristalsis of animal, promote to digest and assimilate.
Cellulase (the English:cellulase) be enzyme one kind, in decomposition of cellulose from living things catalysis make
With.Being can be by cellulose decomposition at oligosaccharides or the protein of monosaccharide.Cellulase is widely present in the organism of nature.
Bacterium, fungi can generate cellulase in animal body etc..The cellulase for being generally used for production comes from fungi, compares allusion quotation
Type has trichoderma (Trichoderma), aspergillus (Aspergillus) and Penicillium (Penicillium).
EM bacterium（EffectiveMicroorganisms）It is by about 80 kinds of microorganism groups at EM bacterium are big by Japanese Ryukyu
Ratio is good to be studied successfully professor's nineteen eighty-two according to husband, is put goods on the market the eighties.EM bacterium are with photosynthetic bacteria, lactic acid bacteria, yeast
10 based on bacterium and actinomyces belong to a kind of micro- life bacteria preparation that more than 80 microorganisms are combined.The mechanism of action is to form EM
The competition of bacterium and pathogenic microorganism contention nutrition, since em bacterium easily live and reproduce in the soil, so can it is very fast and steadily
Occupy the ecologic niche in soil, form the advantage group of beneficial microbial bacteria, to control pathogenic microorganism breeding and
Invasion to crop.It is the developing direction of the ecological agriculture, is more advantageous to the sustainable development of agricultural.The beginning of the nineties at the end of the eighties,
EM bacterium by the states such as Japan, Thailand, Brazil, the U.S., Indonesia, Sri Lanka be widely used in agricultural, cultivation, plantation,
The fields such as environmental protection achieve apparent economic benefit and ecological benefits.
Rhodopseudomonas palustris (Rhodopseudanonaspalustris) belong to photosynthetic bacteria (english abbreviation PSB)
One kind is one of microorganism most ancient on the earth.PSB thalline are full of nutrition, and protein content is up to 65%, and marsh is red false single
Born of the same parents Pseudomonas is one of microorganism most ancient on the earth in one kind of photosynthetic bacteria (english abbreviation PSB).PSB thalline nutrition is rich
Richness, protein content is up to 65%, and is rich in various bioactivators, and adaptable strong, is resistant to the organic of high concentration
The poisonous substances such as phenol, cyanogen are also had the features such as certain tolerance and capacity of decomposition by waste water and stronger decomposition and inversion ability.
Candida utili is called Torula utilis Henneb or edible torula, Latin are entitledCandidautilis, Candida utilis
The cell of yeast is rounded, oval or sausage shape, size are（3.5~4.5）um×（7~13）um；Liquid Culture does not produce mould,
Tube bottom has bacterial sediment.Candida utili can utilize molasses, wood hydrolysis liquid etc. to produce the edible protein of people and animals, energy
Animal intestinal micro-ecology balance is adjusted, feed digestibility is improved, enhances animal body immunity.Candida utili can utilize
Cellulose raw material produces nutrition and increases rich fermented feed, and just contains a large amount of cellulose in bagasse.Further, since production protein is false
Silk yeast cells is rich in vitamin B and protein, moreover it is possible to provide the part nutriment needed for animal.As feed enzyme additive
Can generate a variety of enzymes after yeast cell wall cracking, yeast cells living also can a variety of enzymes of exocytosis, such as amylase, albumen
Enzyme, cellulase etc..Thus, yeast can reinforce the digestibility and utilization to nutriment, promote growth of animals or poultry, promote its appetite, increase
The strong ability resisted the disease with resisting stress.
Lactobacillus plantarum is one kind of lactic acid bacteria, and optimum growth temperature is 30-35 DEG C, and anaerobism or amphimicrobian, strain are
Straight or curved is rod-shaped, single, sometimes in pairs or at chain, and optimal pH 6.5 or so belongs to homofermentative lactic bacteria.This bacterium and its
His lactic acid bacteria can largely produce acid, so that the pH value in water is stablized and not increase difference lies in the viable count of this bacterium is relatively high, and
The acidic materials of its output can degrade heavy metal；Since this bacterium is anaerobic bacteria（It is facultative aerobic）, can output in reproductive process
Distinctive lactobacillin, lactobacillin are a kind of preservatives of bion.
Eucalyptus branches and leaves are the branch and leaf of eucalyptus, eucalyptus (EucalyptusrobustaSmith) You Jiali trees are also known as,
It is the general designation of Myrtaceae eucalyptus plant.Evergreen high megaphanerophyte, about more than 600 plant.Aithullium has periodically withered in 1 year
The phenomenon that leaf falls off, big multi items are high megaphanerophytes, and minority is dungarunga, in frutescent seldom.Shape of tree-crown have steeple shape,
Racemosus shape and weeping branch shape etc..Dan Ye, full edge, keratin are had one layer of thin wax sometimes.Leaf can be divided into juvenile leaf, intermediate leaf and
Climax leaves three classes, the leaf of most kinds is to life, smaller, heart-shaped or wealthy lanceolar.Source area is mainly in Australia continent, 19 generation
Discipline is introduced a fine variety to all over the world, by 2012, has 96 countries to have cultivation.There is medicinal, economic dispatch various value.
Wormwood (Classification system Artemisiaargyi), alias wormwood, ice platform, Ai Gao, moxibustion grass, firewood Chinese mugwort, fragrant Chinese mugwort etc., belongs to chrysanthemum
Section artemisia, is herbaceos perennial, and plant has enhanced aroma.Mugwort bitter and it is pungent, it is nontoxic, wash smoked take and all may be used.Energy middle benefit gas,
By cold, dehumidifying, disease is planted by Zhiduo County.《Compendium of Materia Medica》It carries：Chinese mugwort " toast all kinds of diseases and ailments.It can decoct, antiemetic blood diarrhea, lower part is hidden sore, married woman
Leak blood, Li Yin gas；Myogenic meat, wards off chill, has made one son.Make to decoct not enable and see wind, smashes juice stock, only blood trouble, kill roundworm, boiling and ball
It dissipates and appoints.Blood is only collapsed, perianal abscess blood opens up metal-inflicted wound, only abdominal pain, tocolysis.Bitters is decocted, and it is very good to control tinea ... ... ".Modern pharmacology discovery,
Wormwood volatile oil content is more, is mainly 1.8-Cineoles (accounting for 50% or more), camphene, camphor, carvone, trans- reed in oil
Alcohol, α-terpinol, α-absinthol, sesquiterpene alcohols and its ester, aerial part are existing containing a series of sesquiterpenoids derivative
It is proved for experimental study, wormwood has anti-mattress and antivirus action；It relievings asthma, antibechic and phlegm-dispelling functions；Hemostasis and blood coagulation resisting function；
Calm and anti-allergic effects；Protect liver choleretic effect etc..
Utstanding substantial characteristics and significant improvement of the present invention are：
The application prepares White mushroom cultivation base using useless cattle pen bedding and padding as primary raw material, also added with Chinese mesona herb slag, agstone and
Vermiculite power mixing is uniformly put into ferment 25~30 days in tunnel fermentation kiln after, add blackstrap and EM bacterium and be uniformly mixed, ferment 3
~5 days, White mushroom cultivation base is obtained after most laggard disinfection, then plant White mushroom with the White mushroom cultivation base, harvested from being inoculated into
31 days only are needed, mushroom can be adopted 25 days, White mushroom meat is abundant, and delicious flavour is not easy blacking；And fruiting amount is than the cultivation of open source literature
Pei Ji is higher by 1/6th or more.
Specific implementation mode
For the more detailed introduction present invention, with reference to embodiment, the present invention is described further.
Embodiment 1
It uses following steps can be obtained and prepares White mushroom cultivation base by primary raw material of cowboying circle bedding and padding：
（1）50 parts of cattle pen bedding and padding, 15 parts of Chinese mesona herb slag, 5 parts of agstone, 5 parts of vermiculite power, 3 parts of blackstrap, EM are taken by weight
1 part of bacterium；The agstone and vermiculite power be respectively with lime stone and vermiculite smash it through 80 mesh obtained with upper screen cloth it is broken
Powder.Cattle pen bedding and padding are the useless bedding and padding replaced from cattle pen.
（2）Cattle pen bedding and padding, Chinese mesona herb slag, agstone, vermiculite power and EM bacterium are uniformly mixed and obtain premix.
（3）Premix is put into after fermenting 25~30 days in tunnel fermentation kiln, adds blackstrap and EM bacterium are uniformly mixed,
Fermentation 3~5 days, fermentation temperature are controlled at 28 DEG C~30 DEG C.
（4）By the premix after fermentation after pasteurization 10~15 hours, cultivation base is obtained；Sterilisation temp is 60 DEG C
~65 DEG C.After pasteurization, need to be cooled to 25 DEG C or less could be implanted into White mushroom kind for premix after fermentation.The double spores of implantation
Mushroom kind need to be in the moistening soil of culture material upper table cover 4-5cm after 15 days.Moistening soil is to utilize pond sludge, yellow soil and soddy soil
After smashing mixing, clear water is added to being sufficiently humidified so as to obtain the final product, the weight ratio of pond sludge, yellow soil and soddy soil is：3:2:（0.1-
0.5）.
The preparation process of the cattle pen bedding and padding is：
（1）Take 35 parts of the wooden chaff, 25 parts of rice husk, 10 parts of bagasse, 5 parts of sugarcane marrow, 5 parts of fermentation material, forage useless by above-mentioned parts by weight
3 parts of slag, ox urinate 3 parts, 2 parts of the dry slag of cow dung；The forage waste residue, which is that cattle eat grass, expects remaining residue.And by bagasse and forage
Waste residue be ground into granule size be less than 1 centimetre, it is spare.
（2）After mixing by the wooden chaff, rice husk, bagasse, sugarcane marrow, fermentation material and the dry slag of cow dung, the ox of half amount is added
Urine is uniformly mixed, and is left to ferment 3~5 days, is obtained fermentate.
（3）Forage waste residue is added in fermentate to be uniformly mixed, the ox urine for adding the other half amount is uniformly mixed, and stands hair
After ferment 3 days, until fermentate is dry and comfortable to obtain the final product.When the wooden chaff of appearance is hardened during cowboying, harrowed with rotary cultivator or tool loose；Work as bedding and padding
Humidity is excessive when there is peculiar smell, is sprinkling upon on bedding and padding with fermentate, and the bedding and padding per quadrature add 1~2 kilogram.
The fermentation material be add strain using culture medium, the stomach slag and gastric juice that is taken out from ox ruminant stomach, ox urine and ox
The fermentation material that argol slag culture obtains.The culture medium, raw material include following parts by weight：15 parts of the wooden chaff, 10 parts of rice husk, eucalyptus
5 parts of tree branches and leaves particle, 5 parts of sugarcane marrow, 1 part of ferrous sulfate, 2 parts of urea；Eucalyptus branches and leaves particle is to crush granulating using eucalyptus branches and leaves
It spends size and is less than 0.5 centimetre.The strain includes the raw material of following parts by weight：2 parts of saccharomycete, 1 part of bacillus subtilis, fibre
Plain 1 part of the enzyme of dimension, 1 part of EM bacterium, 0.5 part of Rhodopseudomonas palustris, 0.5 part of candida utili, 0.1 part of lactobacillus plantarum.It is described
Fermentation material incubation be by culture medium, strain, ox urine and the dry slag of cow dung be uniformly mixed, culture medium, strain, ox urine and ox
The weight ratio of argol slag is 100:（1~3）:30:20, after one week of stationary culture, the stomach taken out from ox ruminant stomach is added
Slag and gastric juice are uniformly mixed, after standing 3~4 days to get；The weight of the stomach slag and gastric juice and culture medium that are taken out from ox ruminant stomach
Than for（3~4）:100.
Embodiment 2
It uses following steps can be obtained and prepares White mushroom cultivation base by primary raw material of cowboying circle bedding and padding：
（1）Take by weight 55 parts of cattle pen bedding and padding, 20 parts of Chinese mesona herb slag, 10 parts of agstone, 8 parts of vermiculite power, 5 parts of blackstrap,
1.5 parts of EM bacterium；The agstone and vermiculite power is to smash it through 80 mesh with lime stone and vermiculite respectively to obtain with upper screen cloth
Flour.Cattle pen bedding and padding are the useless bedding and padding replaced from cattle pen.
（2）Cattle pen bedding and padding, Chinese mesona herb slag, agstone, vermiculite power and EM bacterium are uniformly mixed and obtain premix.
（3）Premix is put into after fermenting 25~30 days in tunnel fermentation kiln, adds blackstrap and EM bacterium are uniformly mixed,
Fermentation 3~5 days, fermentation temperature are controlled at 28 DEG C~30 DEG C.
（4）By the premix after fermentation after pasteurization 10~15 hours, cultivation base is obtained；Sterilisation temp is 60 DEG C
~65 DEG C.After pasteurization, need to be cooled to 25 DEG C or less could be implanted into White mushroom kind for premix after fermentation.The double spores of implantation
Mushroom kind need to be in the moistening soil of culture material upper table cover 4-5cm after 15 days.Moistening soil is to utilize pond sludge, yellow soil and soddy soil
After smashing mixing, clear water is added to being sufficiently humidified so as to obtain the final product, the weight ratio of pond sludge, yellow soil and soddy soil is：3:2:（0.1-
0.5）.
The preparation process of the cattle pen bedding and padding is：
（1）40 parts of the wooden chaff, 35 parts of rice husk, 20 parts of bagasse, 10 parts of sugarcane marrow, 10 parts of fermentation material, forage are taken by above-mentioned parts by weight
7 parts of waste residue, ox urinate 6 parts, 2.5 parts of the dry slag of cow dung；The forage waste residue, which is that cattle eat grass, expects remaining residue.And by bagasse and
Forage waste residue be ground into granule size be less than 1 centimetre, it is spare.
（2）After mixing by the wooden chaff, rice husk, bagasse, sugarcane marrow, fermentation material and the dry slag of cow dung, the ox of half amount is added
Urine is uniformly mixed, and is left to ferment 3~5 days, is obtained fermentate.
（3）Forage waste residue is added in fermentate to be uniformly mixed, the ox urine for adding the other half amount is uniformly mixed, and stands hair
After ferment 3 days, until fermentate is dry and comfortable to obtain the final product.When the wooden chaff of appearance is hardened during cowboying, harrowed with rotary cultivator or tool loose；Work as bedding and padding
Humidity is excessive when there is peculiar smell, is sprinkling upon on bedding and padding with fermentate, and the bedding and padding per quadrature add 1~2 kilogram.
The fermentation material be add strain using culture medium, the stomach slag and gastric juice that is taken out from ox ruminant stomach, ox urine and ox
The fermentation material that argol slag culture obtains.The culture medium, raw material include following parts by weight：18 parts of the wooden chaff, 12 parts of rice husk, eucalyptus
7 parts of tree branches and leaves particle, 10 parts of sugarcane marrow, 2 parts of ferrous sulfate, 3 parts of urea；Eucalyptus branches and leaves particle is to crush granulating using eucalyptus branches and leaves
It spends size and is less than 0.5 centimetre.The strain includes the raw material of following parts by weight：2 parts of saccharomycete, 1 part of bacillus subtilis, fibre
Plain 1 part of the enzyme of dimension, 1 part of EM bacterium, 0.5 part of Rhodopseudomonas palustris, 0.5 part of candida utili, 0.1 part of lactobacillus plantarum.It is described
Fermentation material incubation be by culture medium, strain, ox urine and the dry slag of cow dung be uniformly mixed, culture medium, strain, ox urine and ox
The weight ratio of argol slag is 100:（1~3）:30:20, after one week of stationary culture, the stomach taken out from ox ruminant stomach is added
Slag and gastric juice are uniformly mixed, after standing 3~4 days to get；The weight of the stomach slag and gastric juice and culture medium that are taken out from ox ruminant stomach
Than for（3~4）:100.
Embodiment 3
It uses following steps can be obtained and prepares White mushroom cultivation base by primary raw material of cowboying circle bedding and padding：
（1）Take by weight 60 parts of cattle pen bedding and padding, 25 parts of Chinese mesona herb slag, 15 parts of agstone, 10 parts of vermiculite power, 8 parts of blackstrap,
2 parts of EM bacterium；The agstone and vermiculite power is smashed it through 80 mesh with lime stone and vermiculite respectively and is obtained with upper screen cloth
Flour.Cattle pen bedding and padding are the useless bedding and padding replaced from cattle pen.
（2）Cattle pen bedding and padding, Chinese mesona herb slag, agstone, vermiculite power and EM bacterium are uniformly mixed and obtain premix.
（3）Premix is put into after fermenting 25~30 days in tunnel fermentation kiln, adds blackstrap and EM bacterium are uniformly mixed,
Fermentation 3~5 days, fermentation temperature are controlled at 28 DEG C~30 DEG C.
（4）By the premix after fermentation after pasteurization 10~15 hours, cultivation base is obtained；Sterilisation temp is 60 DEG C
~65 DEG C.After pasteurization, need to be cooled to 25 DEG C or less could be implanted into White mushroom kind for premix after fermentation.The double spores of implantation
Mushroom kind need to be in the moistening soil of culture material upper table cover 4-5cm after 15 days.Moistening soil is to utilize pond sludge, yellow soil and soddy soil
After smashing mixing, clear water is added to being sufficiently humidified so as to obtain the final product, the weight ratio of pond sludge, yellow soil and soddy soil is：3:2:（0.1-
0.5）.
The preparation process of the cattle pen bedding and padding is：
（1）50 parts of the wooden chaff, 45 parts of rice husk, 25 parts of bagasse, 15 parts of sugarcane marrow, 15 parts of fermentation material, forage are taken by above-mentioned parts by weight
10 parts of waste residue, ox urinate 8 parts, 3 parts of the dry slag of cow dung；The forage waste residue, which is that cattle eat grass, expects remaining residue.And by bagasse and
Forage waste residue be ground into granule size be less than 1 centimetre, it is spare.
（2）After mixing by the wooden chaff, rice husk, bagasse, sugarcane marrow, fermentation material and the dry slag of cow dung, the ox of half amount is added
Urine is uniformly mixed, and is left to ferment 3~5 days, is obtained fermentate.
（3）Forage waste residue is added in fermentate to be uniformly mixed, the ox urine for adding the other half amount is uniformly mixed, and stands hair
After ferment 3 days, until fermentate is dry and comfortable to obtain the final product.When the wooden chaff of appearance is hardened during cowboying, harrowed with rotary cultivator or tool loose；Work as bedding and padding
Humidity is excessive when there is peculiar smell, is sprinkling upon on bedding and padding with fermentate, and the bedding and padding per quadrature add 1~2 kilogram.
The fermentation material be add strain using culture medium, the stomach slag and gastric juice that is taken out from ox ruminant stomach, ox urine and ox
The fermentation material that argol slag culture obtains.The culture medium, raw material include following parts by weight：20 parts of the wooden chaff, 15 parts of rice husk, eucalyptus
10 parts of tree branches and leaves particle, 15 parts of sugarcane marrow, 3 parts of ferrous sulfate, 5 parts of urea；Eucalyptus branches and leaves particle is ground into using eucalyptus branches and leaves
Granule size is less than 0.5 centimetre.The strain includes the raw material of following parts by weight：2 parts of saccharomycete, 1 part of bacillus subtilis,
1 part of cellulase, 1 part of EM bacterium, 0.5 part of Rhodopseudomonas palustris, 0.5 part of candida utili, 0.1 part of lactobacillus plantarum.Institute
The incubation for the fermentation material stated be by culture medium, strain, ox urine and the dry slag of cow dung be uniformly mixed, culture medium, strain, ox urine and
The weight ratio of the dry slag of cow dung is 100:（1~3）:30:20, after one week of stationary culture, it is added and is taken out from ox ruminant stomach
Stomach slag and gastric juice are uniformly mixed, after standing 3~4 days to get；The weight of the stomach slag and gastric juice and culture medium that are taken out from ox ruminant stomach
Measuring ratio is（3~4）:100.
Application Example
1, Guangxi company, the White mushroom culture material for being conducive to the present invention are planted White mushroom, after being implanted into White mushroom kind, can be gone out within 31 days
Mushroom can adopt mushroom 25 days；Specific incomplete statistics, fruiting amount is higher by 1/6th or more than the cultivation base of open source literature, and obtains
White mushroom meat it is abundant, delicious flavour slide it is tender, be not easy blacking.
Above description is not limitation of the present invention, and the present invention is also not limited to examples detailed above, the art it is general
Logical technical staff, in the essential scope of the present invention, the variations, modifications, additions or substitutions made should all belong to the guarantor of the present invention
Protect range.
Claims (7)
1. a kind of method preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding, it is characterised in that：Preparation process is：
（1）Take by weight 50~60 parts of cattle pen bedding and padding, 15~25 parts of Chinese mesona herb slag, 5~15 parts of agstone, vermiculite power 5~
10 parts, 3~8 parts of blackstrap, 1~2 part of EM bacterium；
（2）Cattle pen bedding and padding, Chinese mesona herb slag, agstone, vermiculite power and EM bacterium are uniformly mixed and obtain premix；
（3）Premix is put into after fermenting 25~30 days in tunnel fermentation kiln, adds blackstrap and EM bacterium are uniformly mixed, fermentation
3~5 days, fermentation temperature was controlled at 28 DEG C~30 DEG C；
（4）By the premix after fermentation after pasteurization 10~15 hours, cultivation base is obtained；Sterilisation temp is 60 DEG C~65
℃。
2. the method according to claim 1 for preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding, feature exist
In：The cattle pen bedding and padding include that the raw material of following parts by weight is made：35~50 parts of the wooden chaff, 25~45 parts of rice husk, bagasse
10~25 parts, 5~15 parts of sugarcane marrow, 5~15 parts of fermentation material, 3~10 parts of forage waste residue, ox urinate 3~8 parts, 2~3 parts of the dry slag of cow dung；
The forage waste residue, which is that cattle eat grass, expects remaining residue.
3. the method according to claim 2 for preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding, feature exist
In：The fermentation material is stomach slag and gastric juice, the ox urine and dry slag of cow dung for adding strain using culture medium, being taken out from ox ruminant stomach
Cultivate obtained fermentation material.
4. the method according to claim 3 for preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding, feature exist
In：The culture medium, raw material include following parts by weight：15~20 parts of the wooden chaff, 10~15 parts of rice husk, eucalyptus branches and leaves particle 5
~10 parts, 5~15 parts of sugarcane marrow, 1~3 part of ferrous sulfate, 2~5 parts of urea；Eucalyptus branches and leaves particle is ground into using eucalyptus branches and leaves
Granule size is less than 0.5 centimetre.
5. the method according to claim 3 for preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding, feature exist
In：The strain includes the raw material of following parts by weight：2 parts of saccharomycete, 1 part of bacillus subtilis, 1 part of cellulase, EM bacterium 1
Part, 0.5 part of Rhodopseudomonas palustris, 0.5 part of candida utili, 0.1 part of lactobacillus plantarum.
6. the method according to claim 1 for preparing White mushroom cultivation base as primary raw material using cattle pen bedding and padding, feature exist
In：The agstone and vermiculite power is to smash it through the flour that 80 mesh are obtained with upper screen cloth with lime stone and vermiculite respectively.
7. the method according to claim 1 for preparing White mushroom cultivation base using cattle pen bedding and padding as primary raw material is flat in preparation
Application in mushroom, coprinus comatus, needle mushroom and cultivating champignon base.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108633625A (en) * | 2018-07-30 | 2018-10-12 | 广西仁泰生物科技有限公司 | The method for preparing White mushroom cultivation base as primary raw material using Pleurotus eryngii mushroom bran |
| CN111802176A (en) * | 2020-08-17 | 2020-10-23 | 重庆市农业科学院 | Culture material for coprinus comatus cultivation, preparation method of culture material and coprinus comatus cultivation method |
| CN112154859A (en) * | 2020-08-26 | 2021-01-01 | 河北省科学院生物研究所 | Oyster mushroom cultivation material and preparation method thereof |
| CN112293158A (en) * | 2020-10-26 | 2021-02-02 | 河北省科学院生物研究所 | Edible fungus cultivation material additive and application thereof |
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| CN103449906A (en) * | 2013-08-15 | 2013-12-18 | 安徽中祝农业发展有限公司 | Agaricus bisporus culture medium and preparation method thereof |
| CN104211507A (en) * | 2014-08-27 | 2014-12-17 | 李道德 | Agaricus bisporus culture medium and preparation method thereof |
| CN106386163A (en) * | 2016-08-15 | 2017-02-15 | 韦中定 | Method for cultivating edible fungi by using crop straw |
| CN107867931A (en) * | 2017-11-19 | 2018-04-03 | 广西仁泰生物科技有限公司 | Using White mushroom planting material of the padding for pig sty as primary raw material of giving up |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103449906A (en) * | 2013-08-15 | 2013-12-18 | 安徽中祝农业发展有限公司 | Agaricus bisporus culture medium and preparation method thereof |
| CN104211507A (en) * | 2014-08-27 | 2014-12-17 | 李道德 | Agaricus bisporus culture medium and preparation method thereof |
| CN106386163A (en) * | 2016-08-15 | 2017-02-15 | 韦中定 | Method for cultivating edible fungi by using crop straw |
| CN107867931A (en) * | 2017-11-19 | 2018-04-03 | 广西仁泰生物科技有限公司 | Using White mushroom planting material of the padding for pig sty as primary raw material of giving up |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633625A (en) * | 2018-07-30 | 2018-10-12 | 广西仁泰生物科技有限公司 | The method for preparing White mushroom cultivation base as primary raw material using Pleurotus eryngii mushroom bran |
| CN111802176A (en) * | 2020-08-17 | 2020-10-23 | 重庆市农业科学院 | Culture material for coprinus comatus cultivation, preparation method of culture material and coprinus comatus cultivation method |
| CN112154859A (en) * | 2020-08-26 | 2021-01-01 | 河北省科学院生物研究所 | Oyster mushroom cultivation material and preparation method thereof |
| CN112293158A (en) * | 2020-10-26 | 2021-02-02 | 河北省科学院生物研究所 | Edible fungus cultivation material additive and application thereof |
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