CN100586905C - Plants probiotics composite preparation and preparing technique thereof - Google Patents

Abstract

The invention discloses compound medicament of plant probiotics and a process for preparation, wherein the process employs nature plant tissues as seed source materials, and prepares compound medicament of plant probiotics via steps of eliminated culture in adversity, accumulation culture and dry grinding. The process for preparation of the medicament has the advantages of simple technology, low coasts, perfect effects, wide usage and facility for popularization and sale promotion.

Description

Plants probiotics composite preparation and preparation technology

Technical field

The present invention relates to a kind of plants probiotics composite preparation and preparation technology.

Background technology

It is the boundary science that rise that plant microecology is learned, probiotics-benefit little (abbreviation of useful probiotics) according to microecological regulation and control systems engineering development is successful, the epitome that the little developing direction of representing plants probiotics of benefit also is the plants probiotics generation of the second half in 20th century, grow up, develop.

It is a young subject that the plant microecology of relevant plants probiotics is learned (Plant microecology), also is an ancient subject (Hallman 1999, and Saikkonen 1998).Before more than 2,000 years: the record of kind of the method for turning sour is just arranged in " book of ditch of stagnant water victory " during China Western Han Dynastry.

The use of plants probiotics is started in the 1950's, and the probiotic products that is used for diseases prevention has 5406, G4,1013 etc., and 5406 use area in China reaches 6,700,000 hectares, is world record at that time.

Benefit is little, and (Beneficial Microeeological Preparations BMP), once claimed yield increasing fungus, was the abbreviation of useful probiotics.Be a less investment, the agriculture production new technology of instant effect.(seeing chapter 12 plants probiotics P280 page or leaf in " probiotic bacterium basis and application " book)

The production of probiotic bacterium kind (probiotcs) both at home and abroad at present is extensively to adopt in animal and plant body to separate living fungal component bacillus (Bacillusspp) in the individual plant, its technological process: sample collection → strain separating → biological assay → identification of strains → rejuvenation is short lives, and makes fermented bacterium.This conventional bacterial classification is bred technology and had following defective: the operation sterilizing part of a. culture material and equipment is many; B. increase the cost of investment and the energy consumption of sterilization, temperature control, pressure control, aeration equipment; C. microbial inoculum goods field survivorship competitive capacity weakens, the production application weak effect; D. strain separating technical requirements height is unfavorable for popularizing (seeing chapter 12 plants probiotics seed selection P286 page or leaf in " probiotic bacterium basis and application " book).

Summary of the invention

It is simple to the purpose of this invention is to provide a kind of technology, low cost, and efficient, of many uses, be easy to the plants probiotics preparation that penetration and promotion is used.

Another object of the present invention provides the mixed bacterium of above-mentioned plants probiotics preparation and cultivates production technique.

Technological principle of the present invention is: 1. chemical mutagen and environmental mutagenesis dual-purpose, induce variation bacterial strain; 2. utilize the interaction between microorganism: competition, irrelevant symbiosis, mutualism, commensalism, amensalism and predation (or parasitic) effect, the survival of the fittest produces dominant microflora; 3. add eutrophy, the post-directed training of simulating nature habitat, the environment struggle for existence ability of enhancing dominant microflora.

Technical process of the present invention: sample tissue sampling → substratum is mixed with → mixes mutagenesis cultivation → orientation and adds rich cultivation → drying → pulverizing → inspection after construction → packing.(instrumental analysis detects and does not list technological process in)

Above-mentioned purpose of the present invention is achieved by the following technical solution:

A kind of plants probiotics composite preparation, said preparation adopt following method to prepare:

A, batching: adopt two or more natural phant tissue as planting source material, as nutrient material, mix that to make solid ingredient standby with Semen Maydis powder, rice bran, wheat bran, the peat composed of rotten mosses; It is standby that water intaking again, alum, edible distillate spirit, edible white vinegar, salt and glucose are hybridly prepared into the weak acid treatment solution;

B, adverse circumstance are eliminated and are cultivated: behind above-mentioned standby solid ingredient and the abundant mixing of weak acid treatment solution, and pack into fermentation vat or fermentation bag, compacting, sealing, under 5-30 ℃ of condition, anaerobism was cultivated 1-2 days, surveyed PH≤5.4 o'clock discharging, got the pre-treatment culture material;

C, enrichment culture: get the making beating of bright bean sprouts and grind, adding sugar cane molasses, yeast extract paste, water stir evenly, and are mixed with the short liquid of giving birth to; Short living liquid that will prepare adds and mixes dress pond, back or bag compacting sealing in the pre-treatment culture material, and under 5-30 ℃ of condition, anaerobism was cultivated 3-5 days, detected pH5.5-6.5, discharging when containing bacterium total amount 〉=200,000,000/g;

D, drying and crushing: after the discharging 40-50 ℃ of drying or dry crushing packing.

Described plants probiotics composite preparation is wherein organized respectively as the natural phant of kind of source material and is adopted two or more vegetable material in following each group, and comprises fruit class material and root, stem, leaf class material:

Fruit class material root, stem, leaf class material

First group: soya bean pumpkin sweet potato clover reed wormwood artemisia purslane

Second group: mung bean wax gourd ginger Herba Astragali Melilotoidis (Herba Astragali Sinici) celery fiddlehead

The 3rd group: soya bean watermelon garlic fetters fiber crops Chinese cabbage rye grass

The 4th group: broad bean apple taro akta tealeaves wooden slippers

The 5th group: soya bean strawberry sesame sweet potato reed canary grass reed sesbania

The 6th group: broad bean lotus rhizome chicken head fruit water peanut Radix Dauci Sativae Herba Eichhorniae

In every group between each natural phant tissue proportion relation be any proportioning, be preferably geometric ratio relation.

Described plants probiotics composite preparation, wherein planting source material is the 1-8 weight part, and nutrient material is the 30-80 weight part, and the weak acid treatment solution is the 30-70 weight part, and short living liquid is the 4-10 weight part.

Described plants probiotics composite preparation, wherein nutrient material is got Semen Maydis powder 10-20 part by weight, rice bran 10-20 part, wheat bran 15-25 part, peat composed of rotten mosses 5-15 part.

Described plants probiotics composite preparation, wherein weak acid treatment solution compound method is: get 50 parts of water by weight and add 0.18-0.22 part native alum, 0.08-0.12 the liquor of part commercially available 50 degree, 0.08-0.12 part edible white vinegar, 1.2-1.7 portion salt, 0.3-0.7 part glucose mixes, and regulates pH5.0-6.0 and gets final product.

Described plants probiotics composite preparation, wherein short preparation of giving birth to liquid are to get the bright bean sprouts making beating of 0.3-0.7 part by weight to grind, sugar cane molasses 1.8-2.2 part, and yeast extract paste 0.08-0.12 part adds water and stirs evenly for 5 parts and get final product.

The preparation method of described plants probiotics composite preparation comprises the following steps:

A, batching: adopt two or more natural phant tissue as planting source material, as nutrient material, mix that to make solid ingredient standby with Semen Maydis powder, rice bran, wheat bran, the peat composed of rotten mosses; It is standby that water intaking again, alum, edible distillate spirit, edible white vinegar, salt and glucose are hybridly prepared into the weak acid treatment solution;

B, adverse circumstance are eliminated and are cultivated: behind above-mentioned standby solid ingredient and the abundant mixing of weak acid treatment solution, and pack into fermentation vat or fermentation bag, compacting, sealing, under 5-30 ℃ of condition, anaerobism was cultivated 1-2 days, surveyed PH≤5.4 o'clock discharging, got the pre-treatment culture material;

C, enrichment culture: get the making beating of bright bean sprouts and grind, adding sugar cane molasses, yeast extract paste, water stir evenly, and are mixed with the short liquid of giving birth to; Short living liquid that will prepare adds and mixes dress pond, back or bag compacting sealing in the pre-treatment culture material, and under 5-30 ℃ of condition, anaerobism was cultivated 3-5 days, detected pH5.5-6.5, discharging when containing bacterium total amount 〉=200,000,000/g;

D, drying and crushing: after the discharging 40-50 ℃ of drying or dry crushing packing.

Beneficial effect of the present invention:

One, processing method of the present invention and common process method are carried out process advantage relatively, and the result is as follows:

(1) this processing method

Gather kind of a former culture material → mixing mutagenesis → orientation and add rich cultivation → drying → pulverizing → inspection after construction → encapsulation warehouse-in (7 road production process).

(2) common process method (seeing " probiotic bacterium basis and application " P312-3132002 version in October)

1, liquid fermentation and culture:

Strain separating purification → former actication of culture → strain expanded culture → fermentor cultivation → adding stopping composition → press filtration → drying → pulverizing → inspection after construction → encapsulation warehouse-in (10 procedure high-temperature sterilizations 4 times)

2, solid fermentation is cultivated:

On the filler that strain separating purification → actication of culture → strain expanded culture → Inoculant was sterilized → shallow-layer solid fermentation cultivation → drying → pulverizing → inspection after construction → encapsulation warehouse-in (4 high-temperature sterilizations of 9 road technologies)

3, solid powder fermentation machine is cultivated:

Former strain separating purification → actication of culture → strain expanded culture → solid medium apolegamy → pulverizing → solid fermentation machine → solid powder fermentation machine stereoscopic culture → drying → pulverizing → solid powder formulation → inspection after construction → encapsulation warehouse-in (12 procedures, 3 high-temperature sterilizations).

By comparison as can be seen, technology of the present invention is simple than traditional technology, and cost is low, is suitable for commercial application.

Two, the present invention utilizes plant tissue cell to make kind of a raw material, cultivate through mixed structure, and living fungal component (integrouth endophyte) in the enriching plant, the Technology of preparation plants probiotics preparation also has the following advantages:

1, draw materials conveniently, material source is extensive, also can oneself plant, and raw materials for production are secure;

2, the numerous process of the bent expansion of omission strain separating link and kind in the production, culture material (original seed preparation) is directly used in to produce with planting and reduces production costs;

3, cost and energy consumption are saved in cancellation sterilization, ventilation, temperature control, the facility investment of pressure control link;

4, All Pure Nature is cultivated, and does not use chemosynthesis material and artificial hormone;

5, the preparation of preparation is active strong, and multi-function action is applied widely, can do a. fertilizer fermented bacterium, and consumption is 1-3 ‰; B. edible culture material fermented bacterium, consumption is 1-3 ‰; C. store feed additive, consumption is 1-3 ‰; D. the high yield Organic farming is planted fertilizer, mu 5kg-10kg; E. the soil improvement agent in the circular agriculture, soil sanitation agent, mu 5kg-10kg.

Three, the little effect of plant benefit:

(1), multi-functional efficiently:

1. promotion growth and development of plants; 2. increase plant biological output; 3. the little plant tissue that promotes of benefit changes to the high yield high-quality; 4. the improvement plant quality improves the quality of products; 5. improve the resistance of plant.

(2), adaptability widely.

(3), Gao Du affinity.

(4), security reliably.

Test 1, be used as the fertilizer fermented bacterium

Get 10 tons of the fresh fowl and animal excrements that went too far the same day, add the fermentation vat of packing into after probiotics preparation (by embodiment 1 preparation) 20kg mixes, track tramping to add a cover after the compacting or cover sealing and cultivate and go out the pond airing after 5-8 days with plastic cloth, or under 45 ℃ of low temperature, be dried to moisture less than 35%, crushing packing organic fertilizer product, can be directly used in farm crop fertilizer, or make culture medium of edible fungus, or make the bait material.

Experiment 2, bacterial manure effect

Test site: Tang Gou village, the Liu Xin of Xuzhou City town

Test unit: agrotechnical station, willow Xinxiang

Test period: on March 5th, 2000

For studying thing: Anhui wheat 19

Test area: one mu, treatment zone 150 square meters repeat 150 square meters, and all the other are the check plot

Application process: get probiotic agent (pressing embodiment 1 preparation) 2000 grams, add clear water 50kg and soaked 12 hours

Add clear water 50kg after the filtered juice and spray the wheat complete stool.

Test-results: the treatment zone comparison increases production 7.9% according to every mu of volume increase 33.2kg, and the wheat growing way shows as: 1. ripe lover, and the obvious 2. degeneration small ears of hot-dry wind tolerance reduce 1.3, and empty flat grain reduces 4.2%, and individual heavily increases by 3 grams.

Experiment 3: the effect of probiotics fermention fertilizer on fruit tree

Test site: peach lane, Fu Jia limit, Lishui County, Nanjing, Green Dragon sorb garden

Test unit: Nanjing Xinshiji Inst. of Gardening

Test period: 17, on July of October calendar year 2001-2002

Test materials and method: 1. treatment zone: probiotic bacterium fertilizer (pressing embodiment 1 preparation); Check plot: fresh pig dung.Repeat 2 times.

For studying thing: 20 years living chrysanthemum pears, 17 years living green plums.

Consumption: the fertile every strain 1kg of probiotic bacterium, the every strain 30kg of fresh pig dung.

Test-results: chrysanthemum pears: treatment zone: single fruit weight 258 grams, check plot: single fruit weight 256 grams;

Green plum: treatment zone: 45.8 jin of single plant yields, single fruit weight 21 grams; Check plot: 43.5 jin of single plant yields, single fruit weight 20 grams.

Conclusion: this evidence: 1kg probiotic bacterium fertilizer is equivalent to the fertilizer efficiency of 30kg pig manure, and the fertilizer fertilizer efficiency behind probiotics fermention utilization ratio has then improved 30 times, shows extremely significant fertilizer efficiency effect.

Experiment 4: the effect of probiotics fermention fertilizer on vegetables

Test unit: State Bureau of Environmental Protection Organic food centre of development

Experimental field: Nanjing herds of horses vegetables science and technology station

Test kind: capsicum

Test period: on March 10th, 2000

Test method and content: 0.12 mu of every sub-district, repeat 2 times, treatment zone is probiotic bacterium base manure (pressing embodiment 1 preparation)+biological pesticide control.

The check plot is: 1,240kg cow dung/sub-district+composite fertilizer 6kg/ sub-district, biological pesticide control 2, composite fertilizer 12kg/ sub-district+biological pesticide control

Test-results: probiotic bacterium fertilizer plant height and branches are compared respectively according to increasing by 19.2% and 28.2%, and the setting tree increases by 74.4 than multiple fertile district, and adding composite fertilizer than cow dung increases by 66.7.

Conclusion: cow dung adds composite fertilizer's plant height increment and branch amount is lower than composite fertilizer 11.5 and 23.1 respectively, and the setting height of tree illustrates that in composite fertilizer 7.7% the dried cow dung this season without probiotics fermention does not have the fertilizer efficiency performance.

From above-mentioned experimental study as can be seen: the remarkable effect that probiotic bacterium base manure transforms for the nutrient of fertilizer and to the collaborative promoter action of plant-growth.

Embodiment

The present invention is described further below in conjunction with embodiment:

General explanation:

Used kind of source material of table 1: embodiment taken table

The collection processing of original germ plasm resource: screening natural phant histocyte: select anosis worm health tissues fruit, root, stem, leaf, piece root etc., in time dry, preceding fresh and tender stem, leaf, the root tissue that siccative is ground into 30 order dry powder or will gathers in the crops the same day of batching breaks into slurries.

Embodiment 1

1, batching: take by first group of material in the table 1:

1. plant in the source material fruit class material and get light yellow beans 1kg, sweet potato 1kg, pumpkin 1kg mixes making beating, gets Normal juice 2kg;

2. plant root in the source material, cauline leaf class material is got bright clover 1kg, reed wormwood artemisia 1kg, purslane 1kg mixes making beating, gets Normal juice 2kg;

3. nutrient material is got Semen Maydis powder 15kg, rice bran 15kg, wheat bran 20kg, peat composed of rotten mosses 10kg;

With above-mentioned material mixing, get solid materials, standby.

2, weak acid treatment solution preparation: get 50kg water (preferred natural rainwater or drinkable well water, spring) and add the 0.2kg native alum, the liquor of commercially available 50 degree of 0.1kg, 0.1kg edible white vinegar, 1.5kg salt, 0.5kg glucose mixes, and regulates pH5.4-6.0, and is standby;

3, adverse circumstance eliminate to be cultivated: solid materials and weak acid treatment solution that above-mentioned preparation is standby are fully mixed thoroughly, and pack or dress pond track tramping, compacting, sealing anaerobism normal temperature were cultivated (5～30 ℃) 24-36 hour, survey pH discharging between 4.8-5.4, get the pre-treatment material; Purpose: the aerobic alkali bacterial strain of having a liking for is eliminated in control, brings out the growth that purifying is had a liking for acid, anaerobism and facultative anaerobe.

4, enrichment culture:

A, short preparation of giving birth to liquid: get 0.5kg bright bean sprouts making beating and grind, sugar cane molasses 2kg, yeast extract paste 0.1kg adds water 5kg (preferred natural rainwater or drink well water, spring) and stirs evenly;

B, the short living liquid that will prepare add in the above-mentioned pre-treatment culture material, mix dress pond, back or pack and track tramping the compacting sealing, and under 5～30 ℃ of conditions, anaerobism was cultivated 3～5 days; Discharging detects: pH5.5-6.5 contains bacterium total amount 〉=200,000,000/g.Purpose: the probiotic enriched all living creatures of The purification and rejuvenation is long, strengthens kind of a sexual clorminance.

5, drying and crushing: after the discharging by 45 ℃ of low temperature in time dry or dry, crushing packing.Preparation quality standard: pulvis fineness 〉=80 orders, effective total count 〉=200,000,000/g, assorted bacterium number≤15%, moisture content≤30%.In the water content preferred 18%.

After measured, contain multiple microorganism species in the product of the present invention, on behalf of bacterium, it have:

Ray fungi (actinomycetes): streptomyces (streptomgces), Frankia (frnkia) etc.;

Photosynthetic Azobacter (photosyntheticbacteria PSB): cyanobacteria (cyanobacteria), fixed nitrogen fusobacterium (closteridium), the endogenous spore bacillus (baillus, spp) etc.;

Lactobacillus class (lactobacillus): lactobacillus fermentum (lfermtntum), Lactobacillus buchneri (lbrevis), leukonid (lenconostoc) etc.;

Budding fungus (saccharomyces): geotrichum candidum (geotrichum candidum) etc.; And Mycophyta beneficial microorganism.

Embodiment 2

Plant source material and get the 4th group of material in the table 1, get fruit class material broad bean, each portion of apple, tubers material tealeaves, each portion of wooden slippers, by 1: 1: 1: 1 weight geometric ratio relation was mixed, and got 3kg histogen slurries or 1.5kg and organized dry powder to mix afterwards standby (other process is all undertaken by embodiment 1 operation steps) with 3. nutrilite.

Embodiment 3

After kind of source material was got in the table 1 lotus rhizome, each portion of water peanut of the 6th group and mixed making beating by 1: 1 weight ratio relation, standby after getting histogen slurries 3kg and 3. nutrilite mixing, other process was all undertaken by embodiment 1 operation steps.

Embodiment 4-6

Embodiment 4-6 gets in the table 1 second and third respectively, five groups of materials are all undertaken by the foregoing description 1 operation steps.

Claims (8)

1, a kind of plants probiotics composite preparation is characterized in that said preparation adopts following method to prepare:

A, batching: adopt two or more natural phant tissue as planting source material, as nutrient material, mix that to make solid ingredient standby with Semen Maydis powder, rice bran, wheat bran, the peat composed of rotten mosses; It is standby that water intaking again, alum, edible distillate spirit, edible white vinegar, salt and glucose are hybridly prepared into the weak acid treatment solution;

B, adverse circumstance are eliminated and are cultivated: behind above-mentioned standby solid ingredient and the abundant mixing of weak acid treatment solution, and pack into fermentation vat or fermentation bag, compacting, sealing, under 5-30 ℃ of condition, anaerobism was cultivated 1-2 days, surveyed PH≤5.4 o'clock discharging, got the pre-treatment culture material;

C, enrichment culture: get the making beating of bright bean sprouts and grind, adding sugar cane molasses, yeast extract paste, water stir evenly, and are mixed with the short liquid of giving birth to; Short living liquid that will prepare adds and mixes dress pond, back or bag compacting sealing in the pre-treatment culture material, and under 5-30 ℃ of condition, anaerobism was cultivated 3-5 days, detected pH5.5-6.5, discharging when containing bacterium total amount 〉=200,000,000/g;

D, drying and crushing: after the discharging 40-50 ℃ of drying or dry crushing packing.

2, plants probiotics composite preparation according to claim 1, two or more vegetable material in arbitrary group below it is characterized in that adopting as the natural phant tissue of planting source material, and comprise fruit class material and root, stem, leaf class material:

Fruit class material root, stem, leaf class material

First group: soya bean pumpkin sweet potato clover reed wormwood artemisia purslane

Second group: mung bean wax gourd ginger Herba Astragali Melilotoidis (Herba Astragali Sinici) celery fiddlehead

The 3rd group: soya bean watermelon garlic fetters fiber crops Chinese cabbage rye grass

The 4th group: broad bean apple taro akta tealeaves wooden slippers

The 5th group: soya bean strawberry sesame sweet potato reed canary grass reed sesbania

The 6th group: broad bean lotus rhizome chicken head fruit water peanut Radix Dauci Sativae Herba Eichhorniae

In every group between each natural phant tissue proportion relation be any proportioning.

3, plants probiotics composite preparation according to claim 2 is characterized in that in every group that proportion relation is the geometric ratio relation between each natural phant tissue.

4, plants probiotics composite preparation according to claim 1 is characterized in that kind of source material is the 1-8 weight part, and nutrient material is the 30-80 weight part, and the weak acid treatment solution is the 30-70 weight part, and short living liquid is the 4-10 weight part.

5, plants probiotics composite preparation according to claim 1 is characterized in that nutrient material gets Semen Maydis powder 10-20 part, rice bran 10-20 part, wheat bran 15-25 part, peat composed of rotten mosses 5-15 part by weight.

6, plants probiotics composite preparation according to claim 1, it is characterized in that weak acid treatment solution compound method is: get 50 parts of water by weight and add 0.18-0.22 part native alum, 0.08-0.12 the liquor of part commercially available 50 degree, 0.08-0.12 part edible white vinegar, 1.2-1.7 portion salt, 0.3-0.7 part glucose mixes, and regulates pH5.0-6.0 and gets final product.

7, plants probiotics composite preparation according to claim 1 is characterized in that short preparation of giving birth to liquid is to get the bright bean sprouts making beating of 0.3-0.7 part by weight to grind, sugar cane molasses 1.8-2.2 part, and yeast extract paste 0.08-0.12 part adds water and stirs evenly for 5 parts and get final product.

8, the preparation method of plants probiotics composite preparation according to claim 1 is characterized in that comprising the following steps:

A, batching: adopt two or more natural phant tissue as planting source material, as nutrient material, mix that to make solid ingredient standby with Semen Maydis powder, rice bran, wheat bran, the peat composed of rotten mosses; It is standby that water intaking again, alum, edible distillate spirit, edible white vinegar, salt and glucose are hybridly prepared into the weak acid treatment solution;

B, adverse circumstance are eliminated and are cultivated: behind above-mentioned standby solid ingredient and the abundant mixing of weak acid treatment solution, and pack into fermentation vat or fermentation bag, compacting, sealing, under 5-30 ℃ of condition, anaerobism was cultivated 1-2 days, surveyed PH≤5.4 o'clock discharging, got the pre-treatment culture material;

C, enrichment culture: get the making beating of bright bean sprouts and grind, adding sugar cane molasses, yeast extract paste, water stir evenly, and are mixed with the short liquid of giving birth to; Short living liquid that will prepare adds and mixes dress pond, back or bag compacting sealing in the pre-treatment culture material, and under 5-30 ℃ of condition, anaerobism was cultivated 3-5 days, detected pH5.5-6.5, discharging when containing bacterium total amount 〉=200,000,000/g;

D, drying and crushing: after the discharging 40-50 ℃ of drying or dry crushing packing.