CN104817350A - Preparation method of zinc-iron-rich special biological organic fertilizer for cauliflower - Google Patents
Preparation method of zinc-iron-rich special biological organic fertilizer for cauliflower Download PDFInfo
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Abstract
The invention discloses a preparation method of a zinc-iron-rich special biological organic fertilizer for cauliflower. The method comprises the following steps: 1, preparing zinc-iron-rich cow dung and zinc-iron-rich earthworm manure; 2, preparing a Peru squid enzymatic hydrolysis liquid; 3, adding Aspergillus oryzae, Bacillus subtilis and pseudomonas to raw materials comprising zinc-iron-rich cow dung and rice husk powder, and carrying out primary fermentation to obtain a fermentation product 1; 4, mixing the fermentation product 1 with the Peru squid enzymatic hydrolysis liquid and the zinc-iron-rich earthworm manure, inoculating Azotobacter chroococcum, Bacillus megaterium and Bacillus mucilaginosus, and carrying out secondary fermentation; and 5, carrying out air drying after the fermentation, crushing, and carrying out sealed preservation. The biological organic fertilizer prepared through the method has efficient and abundant zinc, iron and other nutrition elements, is in favor of realizing absorption and utilization of zinc and iron components in cauliflower, improves the plantation quality and the output of cauliflower, is in favor of improving the quality of soil, and improves the ecology benefit.
Description
Technical field:
The present invention relates to agricultural fertilizer field, particularly relate to a kind of preparation method of the fertilizer containing insecticide ingredient, be specially a kind of preparation method of rich zinc-iron Cauliflower bio-organic fertilizer special.
Background technology:
Cauliflower (Brassia oleraceavar.botrytis L.), also known as cauliflower, cauliflower or cauliflower, is a kind of brassicaceous vegetable, is the mutation of wild cabbage.The head of Cauliflower is white inflorescence, similar with the head of Caulis et Folium Brassicae capitatae.Cauliflower rich vitamin B group, C group, easy decomposes and running off, so the unsuitable high-temperature cooking of Cauliflower, is also not suitable for poach.Former Mediterranean, the bouquet that its product organ is pure white, cripetura, fertile tender bud, spray, floral axis etc. is polymerized, be that a kind of crude fiber content is few, quality is fresh and tender, nutritious, and local flavor is delicious, the vegetables of people's eating.Often edible can heat-clearing quench one's thirst, diuresis defaecation, to prevention kinds cancer, there is positive effect simultaneously.Nowadays, Cauliflower is listed in the anticancer recipe among people's ordinary meal by various countries nutritionist, and become one of vegetables that people like, planting range expands gradually, cultivated area increases year by year.
Chinese invention patent CN103044152A discloses a kind of slow-release fertilizer dedicated and preparation method thereof with the biological particles charcoal Cauliflower that is matrix.With the standby particle charcoal of the agriculture waste biomass-makings such as corn cob, Pericarppium arachidis hypogaeae, rice husk, edible fungi substrate for substrate material, utilize its many micropore, strong adsorptive power, to grow necessary nitrogen, phosphorus, potassium and other nutritive element compounds with Cauliflower, make particulate biomass charcoal base Cauliflower slow-release fertilizer dedicated.The biological particles charcoal that this present invention uses is the black bio matter particle charcoal particle that agriculture waste biomass are formed after incomplete combustion under low oxygen conditions, there is the multifrequency nature of similar agron, its absorption and keep the very capable of nutrient and moisture, plant growth can also be stimulated, good to the sustained release performance of N P and K, the slow-release fertilizer applied once prepared thus, effectively can supply Cauliflower whole vegetative period to the demand of nutrient, and there is increase soil fertility, improve the feature such as Soil structure and high-efficiency environment friendly, be conducive to large-scale promotion application in Cauliflower is produced, this Cauliflower Special slow release fertilizer does not add iron fertilizer and zinc fertilizer according to Cauliflower growth needs, the absorption rate of the zinc-iron element contained in fertilizer itself is low, zinc-iron constituent content in Cauliflower bouquet can not be improved well.
Summary of the invention:
The present invention is directed to prior art Problems existing, a kind of preparation method of rich zinc-iron Cauliflower bio-organic fertilizer special is provided, produce the biological organic fertilizer with efficient abundant zinc-iron nutritive element, be conducive to absorbing of zinc-iron composition in Cauliflower body, improve plantation quality and the output of Cauliflower, there are high economic benefit and ecological benefits.
The invention provides following technical scheme:
A preparation method for rich zinc-iron Cauliflower bio-organic fertilizer special, comprises the following steps:
The first step: prepare rich zinc-iron cow dung and rich zinc-iron wormcast;
Second step: prepare Peru squid enzymolysis solution;
3rd step: carry out first time fermentation with rich zinc-iron cow dung and powdered rice hulls for raw material adds viride, aspergillus oryzae, subtilis and pseudomonas, obtain fermentation material one;
4th step: inoculate azotobacter chroococcum, bacillus megaterium and bacillusmusilaginosiengineering after being mixed with Peru squid enzymolysis solution and rich zinc-iron wormcast by fermentation material one and carry out Secondary Fermentation;
5th step: after fermentation ends, air-dry fragmentation sealing is preserved.
Preferably, in the described the first step, the preparation process of rich zinc-iron cow dung is that ox Dietary Supplemented Zinc is fertile and iron fertilizer, in Zn zinc fertilizer addition for 260mg/kg, in Fe iron fertilizer addition for 220mg/kg, under collecting the forage feed of this interpolation zinc fertilizer and iron fertilizer, obtain cattle and sheep ight soil material.
Preferably, in the described the first step, the preparation process of rich zinc-iron wormcast is:
A. chicken manure is dried rear interpolation zinc fertilizer and iron fertilizer, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, mix and regulate moisture content to be 60-65%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 2-2.1 kilogram after putting in order every other day, and earthworm kind loves earthworm (Eisenia fetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc excrement after being completed by earthworm ight soil sample collection.
Preferably, in described second step, the preparation process of Peru squid enzymolysis solution is:
The water adding 3-5 times of Peru squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease (20000U/g) mixes with weight ratio 1:2 with Sumizyme MP (20000U/g), add in Peru squid slurry, addition is the 1.3-1.5% of Peru squid slurry quality, under 52-55 DEG C of water bath condition, individual hour of enzymolysis 7-8, obtains Peru squid enzymolysis solution after enzymolysis terminates.
Preferably, in described 3rd step, rich zinc-iron cow dung and powdered rice hulls mix with dry weight ratio 10:1, add urea and regulate C/N than being 30:1, adding water regulates moisture content to be 65%, inoculation viride, aspergillus oryzae, subtilis and pseudomonas are fermented, inoculum size is respectively 1%, 1%, 1.5%, 1.5%, and fermentation time is 13-15 days, and turning in every 3 days once.
Preferably, in described 4th step, fermentation material one inoculates brown vinelandii, bacillus megaterium and bacillusmusilaginosiengineering after mixing according to weight ratio 20:5:3 with Peru squid enzymolysis solution and rich zinc-iron wormcast, inoculum size is 0.5%, 1%, 1%, and fermentation time is 3-5 days.
Preferably, air-dry after fermentation ends in described 5th step is 15-18% to moisture content, pulverizes 40 mesh sieves, and pack sealing is preserved.
Preferably, the addition of described interpolation viride, aspergillus oryzae, subtilis, pseudomonas, azotobacter chroococcum, bacillus megaterium, bacillusmusilaginosiengineering all with living bacteria count gauge, number of viable >=500,000,000/gram.
The feature of biological organic fertilizer: 1 improves chemical fertilizer utilization ratio, and biological organic fertilizer has the effects such as fixed nitrogen, phosphorus decomposing, potassium decomposing, greatly can improve the utilization ratio of chemical fertilizer.2 improvement soil, increase soil fertility, in biological organic fertilizer, not only organic component content enriches, in addition also containing be conducive to edatope improve probiotics.After soil application biological organic fertilizer, add the content of the nutritive substances such as the organic matter of soil, nitrogen, phosphorus, potassium to a great extent, phosphorus decomposing in fertilizer, potassium solubilizing bacteria play its phosphorus decomposing, potassium decomposing effect in soil, phosphoric in soil and potassium element are discharged, adds the available content of mineral substances of plant in soil.On the other hand, because biological organic fertilizer is applied in soil, add the organic content in soil, make that soil becomes loose, nutrient laden, the original flora lived in soil obtains amount reproduction, thus ensure that the micro-ecological environment of soil.3 alleviate environmental pollution, and according to statistics, have 1.4 hundred million tons to be burned in the agricultural crop straw of more than 600,000,000 ton that China produces every year, the burning of stalk not only causes the waste of organic substance, also creates atmospheric pollution.These discarded stalks are used and are fermented into biological organic fertilizer, huge economic benefit and the protection of air will be produced to agricultural.Use biological organic fertilizer can improve soil quality and soil microenvironment.4 improve crop quality, and the multiple nutritional components that biological organic fertilizer is rich in imparts the special fertilizer efficiency of fertilizer, proves after deliberation, and biological organic fertilizer can improve color and luster, the quality such as mouthfeel and growth vigor of crop.5 alleviate crop pests, and one is the bacteriostatic action of biological organic fertilizer self, and two is effect of biological organic fertilizer on crops growth, antibacterial, disease-resistant promoter action.
Beneficial effect of the present invention is:
1. the inventive method prepares rich zinc-iron Cauliflower bio-organic fertilizer special, efficient and healthful, by the fermentative action of microorganism, increases solubility humic acid material, solubility humic acid material can form highly stable title complex with zinc-iron, increase the validity of zinc-iron, zinc-iron constituent content in Cauliflower can be improved, improve Cauliflower quality, and reduce disease, improve output, there are good economic benefit and ecological benefits, should be widely promoted.
2. the present invention contains multiple effective microbe, retain the good function characteristic that general fertilizer has, can promote Cauliflower to nutritive element absorb and tangerine resists the pathogenic effects of some pathogenic micro-organism, alleviate the disease and pest in Cauliflower planting process, improve plantation quality and output.
3. the microbial organic fertilizer that the inventive method prepares meets Cauliflower and to grow required nutritional need, and containing enriching efficient zinc-iron trace element, being conducive to Cauliflower and growing, having good economy and zoology benifit.
Embodiment:
Be described in detail embodiments of the invention below, the present embodiment is implemented under premised on invention technical scheme, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.The experimental program of unreceipted actual conditions in embodiment, the condition that conveniently condition or manufacturers advise usually is implemented.
Embodiment one
A preparation method for rich zinc-iron Cauliflower bio-organic fertilizer special, comprises the following steps:
The first step: prepare rich zinc-iron cow dung and rich zinc-iron wormcast:
The preparation process of rich zinc-iron cow dung is that ox Dietary Supplemented Zinc is fertile and iron fertilizer, in the addition of Zn zinc fertilizer zinc chloride for 260mg/kg, in the addition of Fe iron fertilizer ferric sulfate for 220mg/kg, under collecting the forage feed of this interpolation zinc fertilizer and iron fertilizer, obtain cattle and sheep ight soil material;
The preparation process of rich zinc-iron wormcast is:
A. chicken manure is dried rear interpolation zinc fertilizer and iron fertilizer, in Zn zinc fertilizer zinc chloride addition for 250mg/kg, in the addition of Fe iron fertilizer ferric sulfate for 200mg/kg, mix and regulate moisture content to be 60%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 2 kilograms every other day after putting in order, and earthworm kind loves earthworm (Eiseniafetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc excrement after being completed by earthworm ight soil sample collection.
Second step: prepare Peru squid enzymolysis solution:
The water adding 3 times of Peru squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is 1.3% of Peru squid slurry quality, enzymolysis 7 hours under 52 DEG C of water bath condition, obtains Peru squid enzymolysis solution after enzymolysis terminates.
3rd step: rich zinc-iron cow dung and powdered rice hulls mix with dry weight ratio 10:1, add urea and regulate C/N than being 30:1, adding water regulates moisture content to be 65%, add viride (Trichoderma viride), aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillus subtilis) and pseudomonas (Pseudomonas sp.), inoculum size is respectively 1%, 1%, 1.5%, 1.5%, fermentation time is 13 days, turning in every 3 days once carries out one time fermentation, obtains fermentation material one.
4th step: fermentation material one is mixed according to weight ratio 20:5:3 with Peru squid enzymolysis solution and rich zinc-iron wormcast, inoculation azotobacter chroococcum (Azotobacter chroococcum), bacillus megaterium (Bacillusmegatherium) and bacillusmusilaginosiengineering (Bacillus mucilaginosus), inoculum size is 0.5%, 1%, 1%, fermentation time is 3 days, carries out Secondary Fermentation;
5th step: air-dry after fermentation ends is 15% to moisture content, pulverizes 40 mesh sieves, pack sealing is preserved.
Embodiment two
A preparation method for rich zinc-iron Cauliflower bio-organic fertilizer special, comprises the following steps:
The first step: prepare rich zinc-iron cow dung and rich zinc-iron wormcast:
The preparation process of rich zinc-iron cow dung is that ox Dietary Supplemented Zinc is fertile and iron fertilizer, in Zn zinc fertilizer zinc chloride addition for 260mg/kg, the addition that Fe counts iron fertilizer ferric sulfate is 220mg/kg, obtains cattle and sheep ight soil material under collecting the forage feed of this interpolation zinc fertilizer zinc chloride and iron fertilizer;
The preparation process of rich zinc-iron wormcast is:
A. chicken manure is dried rear interpolation zinc fertilizer and iron fertilizer, in Zn zinc fertilizer zinc chloride addition for 250mg/kg, in the addition of Fe iron fertilizer ferric sulfate for 200mg/kg, mix and regulate moisture content to be 65%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 2.1 kilograms every other day after putting in order, and earthworm kind loves earthworm (Eisenia fetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc excrement after being completed by earthworm ight soil sample collection.
Second step: prepare Peru squid enzymolysis solution:
The water adding 5 times of Peru squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is 1.5% of Peru squid slurry quality, enzymolysis 8 hours under 55 DEG C of water bath condition, obtains Peru squid enzymolysis solution after enzymolysis terminates.
3rd step: rich zinc-iron cow dung and powdered rice hulls mix with dry weight ratio 10:1, add urea and regulate C/N than being 30:1, adding water regulates moisture content to be 65%, add viride (Trichoderma viride), aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillus subtilis) and pseudomonas (Pseudomonas sp.), inoculum size is respectively 1%, 1%, 1.5%, 1.5%, fermentation time is 15 days, turning in every 3 days once carries out one time fermentation, obtains fermentation material one.
4th step: fermentation material one is mixed according to weight ratio 20:5:3 with Peru squid enzymolysis solution and rich zinc-iron wormcast, inoculation azotobacter chroococcum (Azotobacter chroococcum), bacillus megaterium (Bacillusmegatherium) and bacillusmusilaginosiengineering (Bacillus mucilaginosus), inoculum size is 0.5%, 1%, 1%, fermentation time is 5 days, carries out Secondary Fermentation;
5th step: air-dry after fermentation ends is 18% to moisture content, pulverizes 40 mesh sieves, pack sealing is preserved.
Embodiment three
A preparation method for rich zinc-iron Cauliflower bio-organic fertilizer special, comprises the following steps:
The first step: prepare rich zinc-iron cow dung and rich zinc-iron wormcast:
The preparation process of rich zinc-iron cow dung is by fertile for ox Dietary Supplemented Zinc zinc chloride and iron fertilizer, in Zn zinc fertilizer zinc sulphide addition for 260mg/kg, in the addition of Fe iron fertilizer ferric sulfate for 220mg/kg, under collecting the forage feed of this interpolation zinc fertilizer zinc chloride and iron fertilizer, obtain cattle and sheep ight soil material;
The preparation process of rich zinc-iron wormcast is:
A. chicken manure is dried rear interpolation zinc fertilizer zinc chloride and iron fertilizer, in Zn zinc fertilizer zinc sulphide addition for 250mg/kg, in the addition of Fe iron fertilizer ferric sulfate for 200mg/kg, mix and regulate moisture content to be 63%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 2.1 kilograms every other day after putting in order, and earthworm kind loves earthworm (Eisenia fetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc excrement after being completed by earthworm ight soil sample collection.
Second step: prepare Peru squid enzymolysis solution:
The water adding 4 times of Peru squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is 1.4% of Peru squid slurry quality, enzymolysis 8 hours under 52-55 DEG C of water bath condition, obtains Peru squid enzymolysis solution after enzymolysis terminates.
3rd step: rich zinc-iron cow dung and powdered rice hulls mix with dry weight ratio 10:1, add urea and regulate C/N than being 30:1, adding water regulates moisture content to be 65%, add viride (Trichoderma viride), aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillus subtilis) and pseudomonas (Pseudomonas sp.), inoculum size is respectively 1%, 1%, 1.5%, 1.5%, fermentation time is 14 days, turning in every 3 days once carries out one time fermentation, obtains fermentation material one.
4th step: fermentation material one is mixed according to weight ratio 20:5:3 with Peru squid enzymolysis solution and rich zinc-iron wormcast, inoculation azotobacter chroococcum (Azotobacter chroococcum), bacillus megaterium (Bacillusmegatherium) and bacillusmusilaginosiengineering (Bacillus mucilaginosus), inoculum size is 0.5%, 1%, 1%, fermentation time is 4 days, carries out Secondary Fermentation;
5th step: air-dry after fermentation ends is 17% to moisture content, pulverizes 40 mesh sieves, pack sealing is preserved.
Embodiment four
A preparation method for rich zinc-iron Cauliflower bio-organic fertilizer special, comprises the following steps:
The first step: prepare rich zinc-iron cow dung and rich zinc-iron wormcast:
The preparation process of rich zinc-iron cow dung is that ox Dietary Supplemented Zinc is fertile and iron fertilizer, in Zn zinc fertilizer zinc chloride addition for 260mg/kg, in Fe iron fertilizer ferric sulfate addition for 220mg/kg, under collecting the forage feed of this interpolation zinc fertilizer zinc chloride and iron fertilizer, obtain cattle and sheep ight soil material;
The preparation process of rich zinc-iron wormcast is:
A. chicken manure is dried rear interpolation zinc fertilizer and iron fertilizer, in Zn zinc fertilizer zinc chloride addition for 250mg/kg, in the addition of Fe iron fertilizer ferric sulfate for 200mg/kg, mix and regulate moisture content to be 61%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 2.1 kilograms every other day after putting in order, and earthworm kind loves earthworm (Eisenia fetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc excrement after being completed by earthworm ight soil sample collection.
Second step: prepare Peru squid enzymolysis solution:
The water adding 5 times of Peru squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is 1.3% of Peru squid slurry quality, enzymolysis 8 hours under 52-55 DEG C of water bath condition, obtains Peru squid enzymolysis solution after enzymolysis terminates.
3rd step: rich zinc-iron cow dung and powdered rice hulls mix with dry weight ratio 10:1, add urea and regulate C/N than being 30:1, adding water regulates moisture content to be 65%, add viride (Trichoderma viride), aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillus subtilis) and pseudomonas (Pseudomonas sp.), inoculum size is respectively 1%, 1%, 1.5%, 1.5%, fermentation time is 15 days, turning in every 3 days once carries out one time fermentation, obtains fermentation material one.
4th step: fermentation material one is mixed according to weight ratio 20:5:3 with Peru squid enzymolysis solution and rich zinc-iron wormcast, inoculation azotobacter chroococcum (Azotobacter chroococcum), bacillus megaterium (Bacillusmegatherium) and bacillusmusilaginosiengineering (Bacillus mucilaginosus), inoculum size is 0.5%, 1%, 1%, fermentation time is 3 days, carries out Secondary Fermentation;
5th step: air-dry after fermentation ends is 18% to moisture content, pulverizes 40 mesh sieves, pack sealing is preserved.
Add described in embodiment one to four viride, aspergillus oryzae, subtilis, pseudomonas, azotobacter chroococcum, bacillus megaterium, bacillusmusilaginosiengineering addition all with living bacteria count gauge, number of viable >=500,000,000/gram.
According to the using method activation of described specification sheets and use after purchase, addition is converted into living bacteria count amount to be added, bacillus megaterium number of viable >=1,000,000,000 such as bought/gram, then effective viable bacteria content of this bacteria preparation is 2 times of 500,000,000/gram bacillus megaterium agent, then its addition is 0.5 times of 500,000,000/gram bacillus megaterium.
Planting experiment one
Being tested in March September to next year, carry out on Shanghai Chongming island, is marine solonchak for examination soil, and after planting front and results Cauliflower, soil physical chemistry matter index is in table one.
18 fritters are divided into for examination soil, every three pieces divide one group at random, be divided into six groups, use the rich zinc-iron Cauliflower bio-organic fertilizer special that preparation method described in embodiment one to four prepares respectively and make base manure, be denoted as treatment group one to four group, amount of application is 40kg/ mu, and use Cauliflower applicable biological fertilizer and selenium fertilizer and iron fertilizer that market is bought for remaining two pieces, amount of application is used according to purchase specification sheets.
Varieties of plant is the 160 days Cauliflowers in Chongming, belongs to Mid-late ripening and to survive the winter cauliflower variety.Cauliflower is provided by Modern Agricultural Park, Chongming.
Statistics gathers in the crops zinc-iron constituent content in rear Yield of Cauliflower and Cauliflower bouquet, and statistics is in table two, and vitamins C, soluble sugar and nitrate content in statistics results Cauliflower bouquet simultaneously, statistics is in table three.
Table one: planting experiment one soil basic physics and chemistry matter index
Described data are expressed in the mode of mean number ± variance.
Table two: zinc-iron constituent content in Yield of Cauliflower and Cauliflower bouquet
| Planting experiment one | Output (thousand grams/acre) | Zinc element content (mg/kg) | Iron content (mg/kg) |
| Treatment group one | 2254.23±285.63 | 5.69±0.25 | 15.68±2.36 |
| Treatment group two | 2362.14±189.63 | 6.25±0.69 | 19.57±2.10 |
| Treatment group three | 2158.97±222.51 | 4.98±0.78 | 19.63±2.04 |
| Treatment group four | 2336.96±207.95 | 5.28±1.21 | 20.36±3.25 |
| Control group one | 1826.35±306.47 | 3.36±0.96 | 13.25±3.24 |
| Control group two | 2047.85±257.84 | 3.87±0.99 | 12.58±3.36 |
Described data are expressed in the mode of mean number ± variance.
Table three: vitamins C, soluble sugar and nitrate content in Cauliflower bouquet
State data to express in the mode of mean number ± variance
Planting experiment two
Carrying out in Hefei ,Anhui, is another name for Sichuan Province loess for examination soil, and after planting front and results Cauliflower, soil physical chemistry matter index is in table four.
18 fritters are divided into for examination soil, every three pieces divide one group at random, be divided into six groups, use the rich zinc-iron Cauliflower bio-organic fertilizer special that preparation method described in embodiment one to four prepares respectively and make base manure, be denoted as treatment group one to four group, amount of application is 60kg/ mu, uses Cauliflower applicable biological fertilizer and zinc fertilizer and iron fertilizer that market is bought for remaining two pieces, and amount of application is used according to the bright book of purchase.
Examination experimental cultivar is supplied to be 120 days Cauliflowers, outdoor cropping.Planting density is 60,000 strain/hm
2, statistics gathers in the crops zinc-iron constituent content in rear Yield of Cauliflower and Cauliflower bouquet, and statistics is in table five.
Table four: planting experiment two soil basic physics and chemistry matter index
State data to express in the mode of mean number ± variance
Table five: zinc-iron constituent content in Yield of Cauliflower and Cauliflower bouquet
| Planting experiment two | Output (thousand grams/acre) | Zinc element content (mg/kg) | Iron content (mg/kg) |
| Treatment group one | 2883.35±203.36 | 5.25±1.02 | 20.58±1.20 |
| Treatment group two | 2801.47±332.25 | 4.69±0.98 | 19.87±2.09 |
| Treatment group three | 2786.69±307.41 | 5.05±1.28 | 18.54±3.33 |
| Treatment group four | 2778.21±296.51 | 5.28±1.21 | 21.03±2.25 |
| Control group one | 2101.45±401.23 | 3.36±0.96 | 12.58±2.57 |
| Control group two | 2032.14±442.17 | 2.25±0.87 | 9.63±2.84 |
Table six: vitamins C, soluble sugar and nitrate content in Cauliflower bouquet
Described data are expressed in the mode of mean number ± variance.
According to above-mentioned planting experiment data, we can find out, the biological organic fertilizer that the inventive method prepares effectively can improve Cauliflower bouquet zinc-iron constituent content and improve Yield of Cauliflower and quality, more common zinc fertilizer and iron fertilizer, be more conducive to Cauliflower plant absorb, and for vitamins C, soluble sugar and nitrate content in raising Cauliflower bouquet, all there is good effect, simultaneously as biological organic fertilizer, Soil structure can be improved, improve the ecological benefits of plantation, there is well long-range promotional value.
Above content is only better embodiment of the present invention, and for those of ordinary skill in the art, according to thought of the present invention, all will change in specific embodiments and applications, this description should not be construed as limitation of the present invention.
Claims (8)
1. a preparation method for rich zinc-iron Cauliflower bio-organic fertilizer special, is characterized in that, comprise the following steps:
The first step: prepare rich zinc-iron cow dung and rich zinc-iron wormcast;
Second step: prepare Peru squid enzymolysis solution;
3rd step: carry out first time fermentation with rich zinc-iron cow dung and powdered rice hulls for raw material adds viride, aspergillus oryzae, subtilis and pseudomonas, obtain fermentation material one;
4th step: inoculate azotobacter chroococcum, bacillus megaterium and bacillusmusilaginosiengineering after being mixed with Peru squid enzymolysis solution and rich zinc-iron wormcast by fermentation material one and carry out Secondary Fermentation;
5th step: after fermentation ends, air-dry fragmentation sealing is preserved.
2. the preparation method of a kind of rich zinc-iron Cauliflower bio-organic fertilizer special according to claim 1, it is characterized in that: in the described the first step, the preparation process of rich zinc-iron cow dung is that ox Dietary Supplemented Zinc is fertile and iron fertilizer, in Zn zinc fertilizer addition for 260mg/kg, in Fe iron fertilizer addition for 220mg/kg, under collecting the forage feed of this interpolation zinc fertilizer and iron fertilizer, obtain cattle and sheep ight soil material.
3. the preparation method of a kind of rich zinc-iron Cauliflower bio-organic fertilizer special according to claim 1, it is characterized in that, in the described the first step, the preparation process of rich zinc-iron wormcast is:
A. chicken manure is dried rear interpolation zinc fertilizer and iron fertilizer, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, mix and regulate moisture content to be 60-65%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 2-2.1 kilogram after putting in order every other day, and earthworm kind loves earthworm for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc excrement after being completed by earthworm ight soil sample collection.
4. the preparation method of a kind of rich zinc-iron Cauliflower bio-organic fertilizer special according to claim 1, is characterized in that:
In described second step, the preparation process of Peru squid enzymolysis solution is:
The water adding 3-5 times of Peru squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease (20000U/g) mixes with weight ratio 1:2 with Sumizyme MP (20000U/g), add in Peru squid slurry, addition is the 1.3-1.5% of Peru squid slurry quality, under 52-55 DEG C of water bath condition, individual hour of enzymolysis 7-8, obtains Peru squid enzymolysis solution after enzymolysis terminates.
5. the preparation method of a kind of rich zinc-iron Cauliflower bio-organic fertilizer special according to claim 1, it is characterized in that: in described 3rd step, rich zinc-iron cow dung and powdered rice hulls mix with dry weight ratio 10:1, add urea and regulate C/N than being 30:1, adding water regulates moisture content to be 65%, inoculation viride, aspergillus oryzae, subtilis and pseudomonas are fermented, inoculum size is respectively 1%, 1%, 1.5%, 1.5%, and fermentation time is 13-15 days, and turning in every 3 days once.
6. the preparation method of a kind of rich zinc-iron Cauliflower bio-organic fertilizer special according to claim 1, it is characterized in that: in described 4th step, fermentation material one inoculates brown vinelandii, bacillus megaterium and bacillusmusilaginosiengineering after mixing according to weight ratio 20:5:3 with Peru squid enzymolysis solution and rich zinc-iron wormcast, inoculum size is 0.5%, 1%, 1%, and fermentation time is 3-5 days.
7. the preparation method of a kind of rich zinc-iron Cauliflower bio-organic fertilizer special according to claim 1, is characterized in that: air-dry after fermentation ends in described 5th step is 15-18% to moisture content, pulverizes 40 mesh sieves, and pack sealing is preserved.
8. the preparation method of a kind of rich zinc-iron Cauliflower bio-organic fertilizer special according to claim 1, it is characterized in that: the addition of described interpolation viride, aspergillus oryzae, subtilis, pseudomonas, azotobacter chroococcum, bacillus megaterium, bacillusmusilaginosiengineering all with living bacteria count gauge, number of viable >=500,000,000/gram.
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| CN106866195A (en) * | 2017-03-01 | 2017-06-20 | 苏州市相城区石新苗木产销专业合作社 | A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase |
| CN107746326A (en) * | 2017-11-02 | 2018-03-02 | 广西沙田仙人滩农业投资有限公司 | A kind of fertilizer for preventing and treating cauliflower pest and disease damage and preparation method thereof |
| CN108101609A (en) * | 2018-01-19 | 2018-06-01 | 普安县红星村裕丰农果蔬种植农民专业合作社 | A kind of broccoli plantation fertilizer and preparation method thereof |
| CN108220208A (en) * | 2018-03-16 | 2018-06-29 | 青岛农业大学 | A kind of zinc-rich bacterial strain and its application |
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| CN108220208B (en) * | 2018-03-16 | 2020-06-12 | 青岛农业大学 | Zinc-rich bacterial strain and application thereof |
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