CN106866195A - A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase - Google Patents
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase Download PDFInfo
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- CN106866195A CN106866195A CN201710118215.7A CN201710118215A CN106866195A CN 106866195 A CN106866195 A CN 106866195A CN 201710118215 A CN201710118215 A CN 201710118215A CN 106866195 A CN106866195 A CN 106866195A
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- peru squid
- peru
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/002—Fertilisers made from animal corpses, or parts thereof from fish or from fish-wastes
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to plant composite fertilizer's applied technical field, the preparation method that a kind of red kiwi fruit postpones the biological compound fertilizer of picking fruit phase is disclosed, comprised the following steps：The first step：Prepare cow dung and pig manure fermentation material；Second step：Prepare Peru squid enzymolysis liquid；3rd step：By compost fermentation material and Peru's hesitation enzymolysis liquid according to weight than 100 150:Broken sealing preserve is air-dried after 1 mixing, the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase is obtained.The present invention provides a kind of biological compound fertilizer that can postpone the red kiwi fruit picking fruit phase, for the occupation rate of market for improving fruit, improve the economic benefit of plantation, it is significant, composite fertilizer of the present invention can also improve the yield of fruit simultaneously, the incidence of disease of plant is reduced, plantation economic benefit and ecological benefits are improved.
Description
Technical field
Answered with the biology for postponing the picking fruit phase the present invention relates to agricultural fertilizer field, more particularly to a kind of red kiwi fruit
The preparation method in Hefei.
Background technology
Red kiwi fruit also known as red heart Chinese grooseberry/red kiwifruit, characteristic are big in fruit, neat；Fruit is short cylindrical
Shape, pericarp is in green and brown color, without hair.Containing rich in sugar, rich in the several mineral materials such as calcium, iron, potassium and 17 kinds of amino acid, Vc is up to
135.Pulp is emerald green, and the sweet acid of fruit juice is moderate, and pleasant to the palate, quality is extremely excellent.It is direct-edible, can also be adapted to manufacture craft dish
Meat and fish dishes.
The growing environment of Kiwi berry, distribution of the root system in hard soil layer is shallower, can be distributed in loose soil compared with
Deep characteristic, can well-grown in acid, subacidity (soil pH 5.5-6.5) or neutral soil.Existing plurality area
The Kiwi berry of plantation, because of improper administration chemical fertilizer, agricultural chemicals and swelling agent year after year, often leads to that fruit quality is poor, and yielding ability is not strong, produces
Amount is relatively low, and disease resistance poor performance, and such as bacterial canker and fungal leaf spot have a strong impact on the yield and product of Kiwi berry
Matter.
Chinese invention patent CN102060629A is related to a kind of special medicated fertilizer for potatoes and preparation method thereof, the medicine fertilizer to be
It is mixed into agricultural chemicals in organic fertilizer to be made, the formula composition of the agricultural chemicals is：2% kasugarnycin wettable powder, DT wettable powders
Agent, 50% Phoxim emulsion and chlopyrifos emulsion, the mass fraction of each composition is 25-35 parts of 2% kasugarnycin wettable powder,
25-35 parts of DT wettable powders, 50% Phoxim emulsion 15-25 parts and 15-25 parts of chlopyrifos emulsion.By by fertilizer and agricultural chemicals
Organically it is combined together, using more convenient.The underground such as potato black rot and cutworm pest and disease damage can be significantly prevented and treated, is had
Effect ground improves the commodity of potato emergence rate and potato, but the present invention does not have particularity for cauliflower growth, no
Beneficial to the growth demand of cauliflower, while inorganic and organic agricultural chemicals composition is added in the invention, it is unfavorable for the improvement of soil quality, it is right
Ecological environment causes certain adverse effect.
The plucking cycle of current Kiwi berry is short, is typically just plucked in early October and terminated, the Kiwi berry in December-January
Mouthfeel deviation and store high cost.
The content of the invention
A kind of problem that the present invention exists for prior art, there is provided life that can postpone the red kiwi fruit picking fruit phase
Thing composite fertilizer, for the occupation rate of market for improving fruit, improves the economic benefit of plantation, significant, while of the invention
Composite fertilizer can also improve the yield of fruit, reduce the incidence of disease of plant, increase economic efficiency and ecological benefits.
The present invention provides following technical scheme：
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase, comprises the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
10-15g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure is put into after vibration stirring
Sterilizing in steam sterilizer, to open and open ultraviolet after superclean bench and carry out sterilized 10-30min, after after high pressure sterilization,
Take out culture medium to be placed into superclean bench, wait the temperature of culture medium to drop to 60-100 DEG C or so, under sterile conditions, will
Trichoderma viride, aspergillus oryzae are inoculated into culture medium, be then placed in biochemical cultivation case and incubator temperature is adjusted into 37-38 DEG C,
Culture 5-10h, obtains compost fermentation material；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, heap
Fertilizer fermentation 3-4 days；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 1-5 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, addition is dynamic
To in Peru squid slurry, its addition is the 0.5-1.5% of Peru squid slurry quality to thing protease, adds metronidazole solid
Stir, its addition is the 0.1-0.5%, the enzymolysis 7- under 50-60 DEG C of water bath condition of Peru squid slurry quality
10 hours, enzymolysis obtains Peru squid enzymolysis liquid after terminating；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared into 100-150 according to weight:Air-dried after 1 mixing broken
Sealing preserve, obtains the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
Preferably, the first step weighs 15g nutrient broth mediums first in preparing cow dung and pig manure fermentation material
In 500ml distilled water, sterilizing in vertical pressure steam sterilizer is put into after vibration stirring, after opening superclean bench
Opening ultraviolet carries out sterilized 10-20min, is placed into superclean bench after culture medium after high pressure sterilization, is taken out, and waits culture medium
Temperature drop to 60-70 DEG C or so, under sterile conditions, Trichoderma viride, aspergillus oryzae are inoculated into culture medium, be then placed in
37-38 DEG C is adjusted in biochemical cultivation case and by incubator temperature, 8-10h is cultivated, compost fermentation material is obtained.
Preferably, the first step is prepared cultured Trichoderma viride, aspergillus oryzae in cow dung and pig manure fermentation material
It is inoculated into the mixed material that cow dung and pig manure are mixed, inoculum concentration is 1-3g/kg.
Preferably, the second step adds 3-5 times of Peru's squid after preparing and clear up Peru squid in Peru squid enzymolysis liquid
The water of fish weight is smashed to pieces and is made Peru squid slurry, and in addition animal protease to Peru squid slurry, its addition is Peru
The 1.3-1.5% of squid slurry quality, addition metronidazole solid stirs, and its addition is Peru squid slurry quality
0.1-0.3%, the 7-8 hour of enzymolysis under 52-55 DEG C of water bath condition, enzymolysis obtains Peru squid enzymolysis liquid after terminating.
Preferably, compost fermentation material and Peru's hesitation enzymolysis liquid are compared into 100-120 according to weight in the 3rd step:1 mixes
Broken sealing preserve is air-dried after conjunction, the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase is obtained.
The characteristics of biological organic fertilizer：1 improves chemical fertilizer utilization ratio, and biological organic fertilizer has fixed nitrogen, phosphorus decomposing, potassium decomposing etc. to act on, can
To greatly improve the utilization rate of chemical fertilizer.2 improved soils, increase soil fertility, not only organic component content is rich in biological organic fertilizer
Richness, the beneficial bacterium for being also conducive to soil environment to improve in addition.After soil application biological organic fertilizer, largely increased
The content of the nutriments such as organic matter, nitrogen, phosphorus, the potassium of soil, phosphorus decomposing, potassium solubilizing bacteria in fertilizer played in soil its phosphorus decomposing,
Potassium decomposing is acted on, and the P elements and potassium element in soil are discharged, and increased the available content of mineral substances of plant in soil.
On the other hand, because biological organic fertilizer is applied in soil, the organic matter content in soil is increased, soil is become thin
Pine, nutrient laden, the original flora lived in soil obtains amount reproduction, so as to ensure that the micro-ecological environment of soil.3 mitigate
Environmental pollution.4 improve crop quality, and biological organic fertilizer is rich in the multiple nutritional components having and imparts the special fertilizer efficiency of fertilizer, passes through
Research has shown that biological organic fertilizer can improve the qualities such as color and luster, mouthfeel and the growth vigor of crop.5 mitigate crop pests, one
It is the bacteriostasis of biological organic fertilizer itself, two is effect of biological organic fertilizer on crops growth, antibacterial, disease-resistant facilitation.
Metronidazole is nitro imidazole derivatives, can suppress Amoeba redox reaction, protozoon nitrogen chain occurs disconnected
Split.In vitro test is proved, when drug concentration is l-2mg/L, amoeba histolytica can occur morphologic change, 24 in 6-20 hours
All it is killed in hour, when concentration is 0.2g/ml, amoeba histolytica can be killed in 72 hours.The nitro reduction of anaerobic bacteria
Enzyme plays an important role in the energetic supersession of sensitive strain, and the present invention passes through in metronidazole application Peru squid enzymolysis process
Addition metronidazole can significantly improve enzymolysis performance of the animal protease to Peru squid, and the composite bio-fertilizer for preparing can
Significantly to delay the picking fruit phase of red kiwi fruit up to 2-3 month, for regulation market economy benefit, extend Red Male macaque
It is the market share cycle of peach, significant.
Beneficial effects of the present invention are：
1. the present invention can significantly improve animal by metronidazole application Peru squid enzymolysis process by adding metronidazole
Protease can significantly delay the fruit of red kiwi fruit to the enzymolysis performance of Peru squid, the composite bio-fertilizer for preparing
Collecting period, up to 2-3 month, for regulation market economy benefit, extends the market share cycle of red kiwi fruit, with important
Meaning.
2. the invention discloses a kind of biological compound fertilizer that can postpone the red kiwi fruit picking fruit phase, for improving fruit
Real occupation rate of market, improves the economic benefit of plantation, significant, while composite fertilizer of the present invention can also improve fruit
Yield, reduce plant the incidence of disease, improve plantation ecological benefits and economic benefit.
3. composite microbiological fertilizer of the present invention is fermented by During High-Temperature Composting and is made, and can promote kiwi fruit plant to nutrition unit
Element absorb and tangerine resists the pathogenic effects of some pathogenic microorganisms, mitigate the pest and disease damage in Kiwi berry planting process, carry
Height plantation quality and yield, improve economy and zoology benifit.
Specific embodiment
Embodiments of the invention are described in detail below, the present embodiment is carried out under premised on inventive technique scheme
Implement, give detailed implementation method and specific operating process, but protection scope of the present invention is not limited to following realities
Apply example.The experimental program of unreceipted actual conditions in embodiment, generally according to the condition proposed by normal condition or manufacturer
Implement.
Embodiment one
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase, comprises the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
10g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure steam is put into after vibration stirring
Sterilizing in sterilizer, opening ultraviolet carries out sterilized 10min after opening superclean bench, after after high pressure sterilization, takes out training
Foster base is placed into superclean bench, waits the temperature of culture medium to drop to 60 DEG C or so, under sterile conditions, by Trichoderma viride,
Aspergillus oryzae is inoculated into culture medium, is then placed in biochemical cultivation case and incubator temperature is adjusted into 37 DEG C, cultivates 5h, obtains heap
Fertile fermentation material；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, heap
Fertilizer fermentation 3 days, inoculum concentration is 1g/kg；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 1 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, add animal
To in Peru squid slurry, its addition is the 0.5% of Peru squid slurry quality to protease, and the solid stirring of addition metronidazole is equal
Even, its addition is the 0.1% of Peru squid slurry quality, and 7 hours of enzymolysis under 50 DEG C of water bath conditions, enzymolysis terminates
After obtain Peru squid enzymolysis liquid；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared 100 according to weight:Broken sealing is air-dried after 1 mixing
Preserve, obtain the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
Embodiment two
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase, comprises the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
15g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure steam is put into after vibration stirring
Sterilizing in sterilizer, opening ultraviolet carries out sterilized 30min after opening superclean bench, after after high pressure sterilization, takes out training
Foster base is placed into superclean bench, waits the temperature of culture medium to drop to 100 DEG C or so, under sterile conditions, by Trichoderma viride,
Aspergillus oryzae is inoculated into culture medium, is then placed in biochemical cultivation case and incubator temperature is adjusted into 38 DEG C, cultivates 10h, is obtained
Compost fermentation material；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, heap
Fertilizer fermentation 4 days, inoculum concentration is 3g/kg；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 5 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, add animal
To in Peru squid slurry, its addition is the 1.5% of Peru squid slurry quality to protease, and the solid stirring of addition metronidazole is equal
Even, its addition is the 0.5% of Peru squid slurry quality, 10 hours of enzymolysis under 60 DEG C of water bath conditions, enzymolysis knot
Peru squid enzymolysis liquid is obtained after beam；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared 150 according to weight:Broken sealing is air-dried after 1 mixing
Preserve, obtain the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
Embodiment three
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase, comprises the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
15g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure steam is put into after vibration stirring
Sterilizing in sterilizer, opening ultraviolet carries out sterilized 10min after opening superclean bench, after after high pressure sterilization, takes out training
Foster base is placed into superclean bench, waits the temperature of culture medium to drop to 60 DEG C or so, under sterile conditions, by Trichoderma viride,
Aspergillus oryzae is inoculated into culture medium, is then placed in biochemical cultivation case and incubator temperature is adjusted into 37 DEG C, cultivates 8h, obtains heap
Fertile fermentation material；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, heap
Fertilizer fermentation 3-4 days, inoculum concentration is 1g/kg；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 3 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, add animal
To in Peru squid slurry, its addition is the 1.3% of Peru squid slurry quality to protease, and the solid stirring of addition metronidazole is equal
Even, its addition is the 0.1% of Peru squid slurry quality, and 7 hours of enzymolysis under 52 DEG C of water bath conditions, enzymolysis terminates
After obtain Peru squid enzymolysis liquid；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared 100 according to weight:Broken sealing is air-dried after 1 mixing
Preserve, obtain the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
Example IV
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase, comprises the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
15g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure steam is put into after vibration stirring
Sterilizing in sterilizer, opening ultraviolet carries out sterilized 20min after opening superclean bench, after after high pressure sterilization, takes out training
Foster base is placed into superclean bench, waits the temperature of culture medium to drop to 70 DEG C or so, under sterile conditions, by Trichoderma viride,
Aspergillus oryzae is inoculated into culture medium, is then placed in biochemical cultivation case and incubator temperature is adjusted into 37 DEG C, cultivates 10h, is obtained
Compost fermentation material；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, heap
Fertilizer fermentation 3 days, inoculum concentration is 1g/kg；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 5 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, add animal
To in Peru squid slurry, its addition is the 1.5% of Peru squid slurry quality to protease, and the solid stirring of addition metronidazole is equal
Even, its addition is the 0.3% of Peru squid slurry quality, and 8 hours of enzymolysis under 55 DEG C of water bath conditions, enzymolysis terminates
After obtain Peru squid enzymolysis liquid；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared 120 according to weight:Broken sealing is air-dried after 1 mixing
Preserve, obtain the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
Embodiment five
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase, comprises the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
15g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure steam is put into after vibration stirring
Sterilizing in sterilizer, opening ultraviolet carries out sterilized 15min after opening superclean bench, after after high pressure sterilization, takes out training
Foster base is placed into superclean bench, waits the temperature of culture medium to drop to 65 DEG C or so, under sterile conditions, by Trichoderma viride,
Aspergillus oryzae is inoculated into culture medium, is then placed in biochemical cultivation case and incubator temperature is adjusted into 37 DEG C, cultivates 9h, obtains heap
Fertile fermentation material；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, heap
Fertilizer fermentation 3 days, inoculum concentration is 1g/kg；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 4 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, add animal
To in Peru squid slurry, its addition is the 1.4% of Peru squid slurry quality to protease, and the solid stirring of addition metronidazole is equal
Even, its addition is the 0.4% of Peru squid slurry quality, and 8 hours of enzymolysis under 53 DEG C of water bath conditions, enzymolysis terminates
After obtain Peru squid enzymolysis liquid；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared 110 according to weight:Broken sealing is air-dried after 1 mixing
Preserve, obtain the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
Embodiment six
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase, comprises the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
15g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure steam is put into after vibration stirring
Sterilizing in sterilizer, opening ultraviolet carries out sterilized 15min after opening superclean bench, after after high pressure sterilization, takes out training
Foster base is placed into superclean bench, waits the temperature of culture medium to drop to 65 DEG C or so, under sterile conditions, by Trichoderma viride,
Aspergillus oryzae is inoculated into culture medium, is then placed in biochemical cultivation case and incubator temperature is adjusted into 38 DEG C, cultivates 9h, obtains heap
Fertile fermentation material；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, heap
Fertilizer fermentation 3 days, inoculum concentration is 2g/kg；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 4 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, add animal
To in Peru squid slurry, its addition is the 1.3% of Peru squid slurry quality to protease, and the solid stirring of addition metronidazole is equal
Even, its addition is the 0.1% of Peru squid slurry quality, and 8 hours of enzymolysis under 52 DEG C of water bath conditions, enzymolysis terminates
After obtain Peru squid enzymolysis liquid；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared 105 according to weight:Broken sealing is air-dried after 1 mixing
Preserve, obtain the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
Comparative example one
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase, comprises the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
15g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure steam is put into after vibration stirring
Sterilizing in sterilizer, opening ultraviolet carries out sterilized 15min after opening superclean bench, after after high pressure sterilization, takes out training
Foster base is placed into superclean bench, waits the temperature of culture medium to drop to 65 DEG C or so, under sterile conditions, by Trichoderma viride,
Aspergillus oryzae is inoculated into culture medium, is then placed in biochemical cultivation case and incubator temperature is adjusted into 37 DEG C, cultivates 9h, obtains heap
Fertile fermentation material；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, heap
Fertilizer fermentation 3 days, inoculum concentration is 1g/kg；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 4 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, add animal
To in Peru squid slurry, its addition is the 1.4% of Peru squid slurry quality to protease, is digested under 53 DEG C of water bath conditions
8 hours of effect, enzymolysis obtains Peru squid enzymolysis liquid after terminating；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared 110 according to weight:Broken sealing is air-dried after 1 mixing
Preserve, obtain the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
Comparative example two
A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase, comprises the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
15g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure steam is put into after vibration stirring
Sterilizing in sterilizer, opening ultraviolet carries out sterilized 15min after opening superclean bench, after after high pressure sterilization, takes out training
Foster base is placed into superclean bench, waits the temperature of culture medium to drop to 65 DEG C or so, under sterile conditions, by Trichoderma viride,
Aspergillus oryzae is inoculated into culture medium, is then placed in biochemical cultivation case and incubator temperature is adjusted into 38 DEG C, cultivates 9h, obtains heap
Fertile fermentation material, inoculum concentration is 2g/kg；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, heap
Fertilizer fermentation 3 days；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 4 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, add animal
To in Peru squid slurry, its addition is the 1.3% of Peru squid slurry quality to protease, is digested under 52 DEG C of water bath conditions
8 hours of effect, enzymolysis obtains Peru squid enzymolysis liquid after terminating；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared 105 according to weight:Broken sealing is air-dried after 1 mixing
Preserve, obtain the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
Planting experiment one
Ningbo of Zhejiang north logical sequence planting base carries out 4 years seedling planting experiments of red kiwi fruit, and amount of application is 1kg/ plants.Will experiment
Field is randomly divided into 80 parts, then it is one group to extract 10 parts in 80 parts at random, and experimental plot is divided into 8 groups altogether, and embodiment is denoted as respectively
One to six group and comparative example one, two groups of comparative example, kiwi fruit plant picking fruit phase and strain yield of the statistics using different fertilizer.
Table one：Ningbo red kiwi fruit yield, the disease incidence of disease and picking fruit phase
| Planting experiment one | Yield (kilogram/plant) | The incidence of disease (%) | Collecting period |
| Embodiment one | 30.46 | 0.34 | Late Novenber |
| Embodiment two | 32.56 | 0.33 | Late Novenber |
| Embodiment three | 38.13 | 0.35 | The first tenday period of a month December |
| Example IV | 37.31 | 0.34 | The first tenday period of a month December |
| Embodiment five | 39.57 | 0.35 | The first tenday period of a month December |
| Embodiment six | 38.17 | 0.35 | The first tenday period of a month December |
| Comparative example one | 22.25 | 1.46 | Late September |
| Comparative example two | 27.97 | 1.43 | The first tenday period of a month in October |
Planting experiment two
Carried out in Suzhou Dongshan, 3 years seedling planting experiments of red kiwi fruit, amount of application is 1kg/ plants.Experimental plot is divided at random
Into 80 parts, then it is one group to extract 10 parts in 80 parts at random, and experimental plot is divided into 8 groups altogether, and the group of embodiment one to six is denoted as respectively
And comparative example one, two groups of comparative example, kiwi fruit plant picking fruit phase and strain yield of the statistics using different fertilizer.
Table two：Suzhou red kiwi fruit yield, the disease incidence of disease and collecting period
Above content is only better embodiment of the invention, for one of ordinary skill in the art, according to the present invention
Thought, will change in specific embodiments and applications, this specification content should not be construed as to this hair
Bright limitation.
Claims (5)
1. a kind of red kiwi fruit use postpones the preparation method of the biological compound fertilizer of picking fruit phase, it is characterised in that：
Comprise the following steps：
The first step：Prepare cow dung and pig manure fermentation material；
10-15g nutrient broth mediums are weighed first in 500ml distilled water, and vertical pressure steam is put into after vibration stirring
Sterilizing in sterilizer, opening ultraviolet carries out sterilized 10-30min after opening superclean bench, after after high pressure sterilization, takes out
Culture medium is placed into superclean bench, waits the temperature of culture medium to drop to 60-100 DEG C or so, under sterile conditions, by green
Trichoderma, aspergillus oryzae are inoculated into culture medium, be then placed in biochemical cultivation case and incubator temperature is adjusted into 37-38 DEG C, culture
5-10h, obtains compost fermentation material；
Then cultured Trichoderma viride, aspergillus oryzae are inoculated into the mixed material that cow dung and pig manure are mixed, compost hair
Ferment 3-4 days；
Second step：Prepare Peru squid enzymolysis liquid：
Add the water of 1-5 times of Peru squid weight to smash to pieces after Peru squid is cleared up and be made Peru squid slurry, add animal egg
To in Peru squid slurry, its addition is the 0.5-1.5% of Peru squid slurry quality, the solid stirring of addition metronidazole to white enzyme
Uniformly, its addition is the 0.1-0.5% of Peru squid slurry quality, enzymolysis 7-10 under 50-60 DEG C of water bath condition
Hour, enzymolysis obtains Peru squid enzymolysis liquid after terminating；
3rd step：Compost fermentation material and Peru's hesitation enzymolysis liquid are compared into 100-150 according to weight:Broken sealing is air-dried after 1 mixing
Preserve, obtain the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
2. a kind of red kiwi fruit use according to claim 1 postpones the preparation side of the biological compound fertilizer of picking fruit phase
Method, it is characterised in that：
The first step weighs 15g nutrient broth mediums in 500ml distillations first in preparing cow dung and pig manure fermentation material
In water, sterilizing in vertical pressure steam sterilizer is put into after vibration stirring, ultraviolet is opened after opening superclean bench
Sterilized 10-20min is carried out, is placed into superclean bench after culture medium after high pressure sterilization, is taken out, wait the temperature of culture medium to drop to
60-70 DEG C or so, under sterile conditions, Trichoderma viride, aspergillus oryzae are inoculated into culture medium, are then placed in biochemical cultivation case
In and incubator temperature is adjusted to 37-38 DEG C, cultivate 8-10h, obtain compost fermentation material.
3. a kind of red kiwi fruit use according to claim 1 postpones the preparation side of the biological compound fertilizer of picking fruit phase
Method, it is characterised in that：
The first step prepare in cow dung and pig manure fermentation material by cultured Trichoderma viride, aspergillus oryzae be inoculated into cow dung and
In the mixed material that pig manure is mixed, inoculum concentration is 1-3g/kg.
4. a kind of red kiwi fruit use according to claim 1 postpones the preparation side of the biological compound fertilizer of picking fruit phase
Method, it is characterised in that：
The second step is prepared and add the water of 3-5 times of Peru squid weight to smash after Peru squid is cleared up in Peru squid enzymolysis liquid
Broken to be made Peru squid slurry, in addition animal protease to Peru squid slurry, its addition is Peru squid slurry quality
1.3-1.5%, addition metronidazole solid stir, its addition for Peru squid slurry quality 0.1-0.3%,
7-8 hour of enzymolysis under 52-55 DEG C of water bath condition, enzymolysis obtains Peru squid enzymolysis liquid after terminating.
5. a kind of red kiwi fruit use according to claim 1 postpones the preparation side of the biological compound fertilizer of picking fruit phase
Method, it is characterised in that：
Compost fermentation material and Peru's hesitation enzymolysis liquid are compared into 100-120 according to weight in 3rd step:Air-dried after 1 mixing broken
Sealing preserve, obtains the biological compound fertilizer that red kiwi fruit postpones the picking fruit phase.
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| CN201710118215.7A CN106866195A (en) | 2017-03-01 | 2017-03-01 | A kind of red kiwi fruit preparation method of the biological compound fertilizer for postponing the picking fruit phase |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245464A (en) * | 2017-07-13 | 2017-10-13 | 普利资环境科技（苏州）有限公司 | A kind of preparation method of Treatment of Sludge complex micro organism fungicide |
| CN109220466A (en) * | 2018-09-29 | 2019-01-18 | 广西乐业县乐宝电子商务有限公司 | A kind of cultural method extending the Kiwi berry collecting period |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104817366A (en) * | 2015-03-30 | 2015-08-05 | 苏州仁成生物科技有限公司 | Preparation method of biological organic fertilizer for improving storage quality of Fuji apples |
| CN104817350A (en) * | 2015-03-30 | 2015-08-05 | 苏州仁成生物科技有限公司 | Preparation method of zinc-iron-rich special biological organic fertilizer for cauliflower |
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2017
- 2017-03-01 CN CN201710118215.7A patent/CN106866195A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104817366A (en) * | 2015-03-30 | 2015-08-05 | 苏州仁成生物科技有限公司 | Preparation method of biological organic fertilizer for improving storage quality of Fuji apples |
| CN104817350A (en) * | 2015-03-30 | 2015-08-05 | 苏州仁成生物科技有限公司 | Preparation method of zinc-iron-rich special biological organic fertilizer for cauliflower |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245464A (en) * | 2017-07-13 | 2017-10-13 | 普利资环境科技（苏州）有限公司 | A kind of preparation method of Treatment of Sludge complex micro organism fungicide |
| CN109220466A (en) * | 2018-09-29 | 2019-01-18 | 广西乐业县乐宝电子商务有限公司 | A kind of cultural method extending the Kiwi berry collecting period |
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Application publication date: 20170620 |