CN109160862A - A kind of amino acid bio-organic fertilizer and the preparation method and application thereof - Google Patents
A kind of amino acid bio-organic fertilizer and the preparation method and application thereof Download PDFInfo
- Publication number
- CN109160862A CN109160862A CN201811331133.1A CN201811331133A CN109160862A CN 109160862 A CN109160862 A CN 109160862A CN 201811331133 A CN201811331133 A CN 201811331133A CN 109160862 A CN109160862 A CN 109160862A
- Authority
- CN
- China
- Prior art keywords
- organic fertilizer
- fermentation
- preparation
- amino acid
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to a kind of amino acid bio-organic fertilizer, preparation method is as follows: the preparation of S1. fermenting agent: will mix in proportion after bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, Bacillus acidi lactici and aspergillus oryzae liquid fermentation and culture to obtain the final product；S2. glucide and fermenting agent post-fermentation culture is added after Pomacea canaliculata is crushed meat mud in the preparation of aminoacids solution；S3. organic fertilizer prepare: feces of livestock and poultry add system chaff or dregs of rice class raw material after decomposed fermentation to obtain the final product；S4. aminoacids solution is added in organic fertilizer and carries out secondary fermentation up to product of the present invention.The present invention not only can produce a variety of amino acid using complex microorganism degradation Pomacea canaliculata, and microorganism can also breed in fermentation liquid, can be proliferated in organic fertilizer again after secondary fermentation, can improve soil microenvironment after being applied to soil, promote plant growth.
Description
Technical field
The present invention relates to fertilizer fields, and in particular to a kind of amino acid bio-organic fertilizer and the preparation method and application thereof.
Background technique
Pomacea canaliculata introducing in 1981 China, in the 1980s in southern area of China cultivating large area, later because of city
It is bad to be lost one's parents, its feeding habits is wide, adaptable, growth and breeding fastly, lack natural enemy etc. due in nature mass propagation,
The crops such as rice are seriously endangered, cause huge economic loss to agricultural, State Environmental Protection Administration is classified as first batch of 16 kinds of evils
One of property Invasive Alien Species.Pomacea canaliculata individual is big, every 100~150g, and for maximum individual up to 250g or more, the thin meat of shell is more,
Software part is configured to: moisture 81.1%, ash content 2.3%, crude fat 1.1%, crude protein 12.7%, amino acid classes are complete
Contain 16 kinds of aminoacid ingredients.
Amino acid fertilizer is used for crops, can significantly improve crop yield and quality, enhancing crop resistance.In addition, containing ammonia
The fertilizer of base acid can with balanced nutrients, meet the production of green non-pollution, organic products, soil quality can also be improved, improved
Utilization rate of fertilizer reduces pollution.Agricultural aspect mostly obtains amino acid using hair, cotton dregs, sick dead pig at present, produces amino acid fertilizer
Material, amino acid bio activity is lower, cannot be absorbed and utilized well by crops.
In view of the problems of the existing technology, there has been no effective solutions at present.
Summary of the invention
Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a kind of amino acid bio-organic fertilizer and its
Preparation method, the amino acid bio-organic fertilizer mainly carry out Pomacea canaliculata crude protein using microorganism producing enzyme to be decomposed into amino acid,
And combined fertilizer with amino acid by secondary fermentation, the microorganism mass propagation of protein degradation can be made, produced excellent
Matter contains amino acid bio-organic fertilizer.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of preparation method of amino acid bio-organic fertilizer, including following preparation step:
S1. the preparation of fermenting agent
S1-1. the original strain of bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, Bacillus acidi lactici and aspergillus oryzae exists
Respectively successively through inclined-plane culture and liquid fermentation and culture under aseptic condition；
S1-2. by the bacillus subtilis after liquid fermentation and culture, bacillus licheniformis, saccharomyces cerevisiae, Bacillus acidi lactici, rice
Aspergillus mixes in proportion rear up to fermenting agent；
The preparation of S2 aminoacids solution
S2-1. meat mud is crushed with machine after Pomacea canaliculata being rinsed, after the volume mass ratio addition glucide of 1-5%
It is added in fermentor, and carries out sterilization treatment；
S2-2. 0.5-5% fermenting agent as obtained by S1 is added into the fermentor after sterilization treatment, carries out fermentation training
It supports；
S2-2. fermentation liquid is transferred to plastics tank after fermentation, be placed at room temperature for, sealed fermenting 20-30 days up to ammonia
Base acid solution；
S3. the preparation of organic fertilizer
After decomposed fermentation by feces of livestock and poultry plus system chaff or dregs of rice class raw material to obtain the final product；
4) preparation of amino acid bio-organic fertilizer
The resulting aminoacids solution of S2 is added in the resulting organic fertilizer of S3 and carries out secondary fermentation, after fermentation up to ammonia
Base acid biological organic fertilizer；Wherein, aminoacids solution is the 20%-60% of the organic fertilizer weight by envelope-bulk to weight ratio additive amount.
Preferably, in above-mentioned preparation method, bacillus subtilis described in S1-1, bacillus licheniformis, saccharomyces cerevisiae, cream
Acidfast bacilli and the specific inclined-plane culture of aspergillus oryzae and liquid fermentation and culture step are as follows:
Bacillus subtilis, bacillus licheniformis original strain be aseptically inoculated with after NA slant activation respectively
To obtain the final product to LB liquid medium culture 18 hours, condition of culture: ventilatory capacity 2.5kg, revolving speed 180r/min, 30 DEG C of cultivation temperature,
Incubation time 18h；
The original strain of saccharomyces cerevisiae is aseptically seeded to bean sprouts medium after rose-bengal slant activation
Cultivate 28 hours to obtain the final product, condition of culture: ventilatory capacity 0kg, revolving speed 0r/min, 28 DEG C of cultivation temperature, incubation time 28h；
The original strain of Bacillus acidi lactici is aseptically through MRS inclined plane inoculating to MRS fluid nutrient medium culture 28 hours
To obtain the final product, condition of culture: ventilatory capacity 0kg, revolving speed 0r/min, 37 DEG C of cultivation temperature, incubation time 28h；
It is small that the original strain of aspergillus oryzae is aseptically seeded to bean sprouts medium culture 28 after PDA slant activation
When to obtain the final product, condition of culture: ventilatory capacity 2kg, revolving speed 120r/min, 28 DEG C of cultivation temperature, incubation time 28h.
Preferably, in above-mentioned preparation method, bacillus subtilis described in S1-2, bacillus licheniformis, saccharomyces cerevisiae, cream
Acidfast bacilli, aspergillus oryzae by volume 10:10:2:2:3 ratio mixing.
Preferably, in above-mentioned preparation method, glucide described in S2-1 is glucose or sucrose.
Preferably, in above-mentioned preparation method, fermentation culture conditions are revolving speed 225r/min, 28 DEG C of temperature, ventilate in S2-2
0.5kg is measured, ferment 36h.
Preferably, in above-mentioned preparation method, after the volume mass ratio in muddy flesh described in S2-1 by 3% adds glucide
It is added in fermentor；3% fermenting agent as obtained by S1 is added in S2-2 into the fermentor after sterilization treatment, ferments
Culture；Fermentation time is 25 days in S2-3.
Preferably, in above-mentioned preparation method, specific organic fertilizer preparation step includes: that feces of livestock and poultry is added system chaff or the dregs of rice in S3
Class raw material regulates carbon-nitrogen ratio and moisture, and 0.2% commercially available fermenting agent is added, and post-fermentation is uniformly mixed, after fermentation 15-25 days
To obtain the final product, wherein effective bacterium is bacillus subtilis, actinomyces, total living bacteria count >=10,000,000,000/g.
Preferably, in above-mentioned preparation method, secondary fermentation condition described in S4 are as follows: adjust fermentation heap moisture to 40%-
55%, ferment 9-15 days, when moisture be down to 30% hereinafter, to the end of fermentation；
As the same inventive concept, invention further provides a kind of amino acid prepared by the above method is raw
Organic fertilizer.
As the same inventive concept, the present invention also provides a kind of amino acid bio that the above method is prepared is organic
The application of fertilizer, is mainly used in crops；Preferably, the crops are capsicum.Preferably, the amino acid bio-organic fertilizer
Usage amount 100-500kg per acre, it is furthermore preferred that usage amount is 250kg per acre.
Several microorganisms of the present invention are directly to be commercially available, and are purchased from the management of Chinese agriculture Microbiological Culture Collection
Center, wherein the deposit number of bacillus subtilis be ACCC01907, the deposit number of bacillus licheniformis be ACCC04249,
The deposit number of lactic acid bacteria is ACCC10295, the deposit number of S. cervisiae is ACCC20251, the deposit number of aspergillus oryzae
For ACCC30789.
The present invention has the advantage that
1. fermenting agent of the present invention is by bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, Bacillus acidi lactici and meter Qu
The composite bacteria agent of mould five kinds of microbial inoculums composition, mutually collaboration can not occur each other and make during the fermentation for antagonism for five kinds of microbial inoculum combinations
With the generation of promotion strain growth and metabolite；Fermenting agent of the present invention can produce multiple protein enzyme and acid, can drop
The protein and chitin ingredient in Pomacea canaliculata are solved, the amino acid and calcium component in fertilizer, for crop absorption utilization can be increased；Institute
Bacillus subtilis, bacillus licheniformis are stated other than producing protease, has certain antagonism to some fungal diseases, adopts
With complex microorganism degradation Pomacea canaliculata, not only can produce a variety of amino acid (such as aspartic acid, glutamic acid, threonine), it is micro-
Biology can also be bred in fermentation liquid, can be proliferated in organic fertilizer again after secondary fermentation, can be improved soil after being applied to soil
Earth micro-ecological environment inhibits soil-borne disease, promotes plant growth.The present invention mainly utilizes microorganism producing enzyme by the thick egg of Pomacea canaliculata
It is white to carry out being decomposed into amino acid, fertilizer can be combined with amino acid by secondary fermentation, and protein degradation can be made
Microorganism mass propagation, produce high-quality containing amino acid bio-organic fertilizer.Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Without departing substantially from spirit of that invention
In the case where essence, to modifications or substitutions made by the method for the present invention, step or condition, all belong to the scope of the present invention.
Unless otherwise specified, the conventional means that technological means used in embodiment is well known to those skilled in the art；
Unless otherwise specified, agents useful for same is commercially available in embodiment.
Embodiment 1
1) preparation of fermenting agent
Bacillus subtilis, bacillus licheniformis are seeded to LB liquid medium culture 18 hours i.e. after NA slant activation
, condition of culture: ventilatory capacity 2.5kg, revolving speed 180r/min, 30 DEG C of cultivation temperature, incubation time 18h.
Saccharomyces cerevisiae is seeded to bean sprouts medium culture 28 hours to obtain the final product after rose-bengal slant activation, cultivates item
Part: ventilatory capacity 0kg, revolving speed 0r/min, 28 DEG C of cultivation temperature, incubation time 28h.
Bacillus acidi lactici through MRS inclined plane inoculating to MRS fluid nutrient medium culture 28 hours to obtain the final product, condition of culture: ventilatory capacity
0kg, revolving speed 0r/min, 37 DEG C of cultivation temperature, incubation time 28h.
Aspergillus oryzae is seeded to bean sprouts medium culture 28 hours to obtain the final product after PDA slant activation, condition of culture: ventilatory capacity
2kg, revolving speed 120r/min, 28 DEG C of cultivation temperature, incubation time 28h.
By the bacillus subtilis after above-mentioned fermented and cultured, bacillus licheniformis, saccharomyces cerevisiae, Bacillus acidi lactici, aspergillus oryzae
In 10:10:2:2:3 ratio after mixing up to fermenting agent.
2) prepared by aminoacids solution
A. meat mud is crushed with machine after Pomacea canaliculata slightly being rinsed, by 3% volume mass than adding after addition glucose
Into fermentor, by 121 DEG C, 0.1MPa sterilizing 30min；
B. be added 3% fermenting agent, adjustings fermentation condition be revolving speed 225r/min, 28 DEG C of temperature, ventilatory capacity 0.5kg,
Ferment 36h；
C. fermentation liquid is transferred to plastics tank, be placed at room temperature for, sealed fermenting 25 days to obtain the final product.
3) preparation of organic fertilizer
7 parts of pig manure, 3 parts of chaff of system are regulated into moisture after mixing, 0.2% commercially available fermenting agent (effective bacterium is added
For bacillus subtilis, actinomyces, total living bacteria count >=10,000,000,000/g) it is uniformly mixed post-fermentation, it ferments 15 days or so, it is pending
Ferment heap temperature by high temperature (60 DEG C or more) be down to 45 DEG C or so, moisture 40% or so up to organic fertilizer of the presently claimed invention.
4) preparation of amino acid bio-organic fertilizer
The resulting aminoacids solution of step 2) is added in the resulting organic fertilizer of step 3), is uniformly mixed, fermentation heap water is adjusted
Divide to 50% or so.Aminoacids solution ferments 12 days, is down to moisture by envelope-bulk to weight ratio additive amount is organic fertilizer weight 40%
30% hereinafter, up to amino acid bio-organic fertilizer finished product after sieving.
Embodiment 2
1) preparation of fermenting agent is the same as embodiment 1；
2) prepared by aminoacids solution
A. meat mud is crushed with machine after Pomacea canaliculata slightly being rinsed, by 2% volume mass than adding after addition glucose
Into fermentor, by 121 DEG C, 0.1MPa sterilizing 30min；
B. 1.5% fermenting agent is added, adjusting fermentation condition is revolving speed 225r/min, 28 DEG C of temperature, ventilatory capacity
0.5kg, ferment 36h；
C. fermentation liquid is transferred to plastics tank, be placed at room temperature for, sealed fermenting 26 days to obtain the final product.
3) preparation of organic fertilizer
With embodiment 1.
4) preparation of biological organic fertilizer
2) the resulting aminoacids solution of step is added in 3) resulting organic fertilizer.Be uniformly mixed, adjust fermentation heap moisture to
50% or so.Aminoacids solution ferments 12 days by envelope-bulk to weight ratio additive amount is organic fertilizer weight 20%, is down to 30% to moisture
Hereinafter, up to amino acid bio-organic fertilizer finished product after sieving.
Embodiment 3
1) preparation of fermenting agent is the same as embodiment 1；
2) prepared by aminoacids solution
A. meat mud is crushed with machine after Pomacea canaliculata slightly being rinsed, by 3% volume mass than adding after addition glucose
Into fermentor, by 121 DEG C, 0.1MPa sterilizing 30min；
B. be added 4% fermenting agent, adjustings fermentation condition be revolving speed 225r/min, 28 DEG C of temperature, ventilatory capacity 0.5kg,
Ferment 36h；
C. fermentation liquid is transferred to plastics tank, be placed at room temperature for, sealed fermenting 24 days to obtain the final product.
3) preparation of organic fertilizer
With embodiment 1.
4) preparation of biological organic fertilizer
2) the resulting aminoacids solution of step is added in 3) resulting organic fertilizer.It is uniformly mixed, aminoacids solution presses volume weight
60% than additive amount for organic fertilizer weight is measured, adjusts fermentation heap moisture to 50% or so.Fermentation 12 days, is down to 30% to moisture
Hereinafter, up to amino acid bio-organic fertilizer finished product after sieving.
A kind of amino acid bio-organic fertilizer field experiment of experimental example 1
1, test objective:
Verify amino acid bio-organic fertilizer effect of fertilizer.
2, test material:
Testing site: field experiment is carried out in Changsha Liuyang City town Bei Sheng oolong village.For examination Soil-rice soil, main agrochemical
Characteristic is shown in Table 1.
Long-term cropping: capsicum.
Main Agrochemical characters of the table 1 for examination soil
3, experimental design and implementation:
If T1, T2, T3, T4, T5, CK tri- processing.
T1 is that amino acid bio-organic fertilizer adds conventional fertilizer application.250kg/ mus of amino acid bio-organic fertilizer, using 1 side of embodiment
Method is made；Conventional fertilizer application scheme are as follows: 30kg/ mus of 15-15-15 compound fertilizer uses as base manure, in due course additional phosphate fertilizer and potassium
Fertilizer.
T2 adds for commercially available common biological organic fertilizer (organic matter >=45%, total nutrient >=5%, living bacteria count >=200,000,000/g)
Conventional fertilizer application.Commercially available common biological organic fertilizer 250kg/ mus；Conventional fertilizer application scheme are as follows: 30kg/ mus of 15-15-15 compound fertilizer, as
Base manure uses, in due course additional phosphate fertilizer and potash fertilizer.
T3 is to add conventional fertilizer application without amino acid bio-organic fertilizer.Without 250kg/ mus of amino acid bio-organic fertilizer, use
1 method and step 3 of embodiment is made, since microbial total bacterium number is generally below the content of 200,000,000/g after compost bacterium is decomposed,
With commercially available biological and ecological methods to prevent plant disease, pests, and erosion function bacterium bacillus subtilis, living bacteria count >=200,000,000/g is supplemented；Conventional fertilizer application scheme are as follows: 15-15-15 is multiple
It Hefei 30kg/ mus, is used as base manure, in due course additional phosphate fertilizer and potash fertilizer.
T4 is containing amino acid bio-organic fertilizer.250kg/ containing amino acid bio-organic fertilizer mus, production method removes amino acid system
Make other inconsistent outer steps of method with embodiment 1, wherein amino acid production is hydrolyzed using the 5moL/L concentrated sulfuric acid, and product has
Effect total viable count is adjusted to >=2 hundred million/g；Conventional fertilizer application scheme are as follows: 30kg/ mus of 15-15-15 compound fertilizer uses as base manure, fits
When additional phosphate fertilizer and potash fertilizer)；
T5 is containing amino acid bio-organic fertilizer.250kg/ containing amino acid bio-organic fertilizer mus, production method is except microorganism
Other inconsistent outer steps of production method are the same as embodiment 1.Wherein microorganism is commercially available biological and ecological methods to prevent plant disease, pests, and erosion function bacterium bacillus subtilis, product
Effective total viable count is adjusted to >=2 hundred million/g；Conventional fertilizer application scheme are as follows: 30kg/ mus of 15-15-15 compound fertilizer uses as base manure,
Additional phosphate fertilizer and potash fertilizer in due course；
CK is blank test, does not apply organic fertilizer, other doses are the same as processing 1.
3 cells of every group of setting, each cell cultivated area are 5 mu, and experimental field equal topography is flat, and soil fertility is uniform,
It is good to pour drainage condition, avoids road, compost place, ditch, pool, overflow, high-lager building and trees shading etc. distinguishingly
Block.Picking and DATA REASONING are carried out after capsicum is mature.
Influence of 2 different disposal of table to capsicum economical character
Influence of 3 different disposal of table to yield of hot pepper
Show that application effect of the amino acid bio-organic fertilizer on pepper planting is more significant by cell experiment, this hair
Bright organic fertilizer can improve the development degree of capsicum, while improve single fruit weight, obvious effect of increasing production, and the more common organic fertilizer of yield can be with
It improves 16.92% (T2 processing), yield 33.92% (CK processing) can be improved compared with Single fertilizer processing, it is relatively raw without amino acid
Organic fertilizer output increased 12.41% (T3 processing) increases production 7.91% (T4 processing) compared with sulphuric acid hydrolysis amino acid scheme, is relatively free of
This programme complex microorganism fertilizer applications output increased 8.81% (T5 processing).The experimental results showed that complex microorganism combines, makes
The amino acid and biological organic fertilizer produced with this programme has preferable effect of increasing production, and amino acid bio-organic fertilizer of the invention has
Preferable application prospect.
Claims (10)
1. a kind of preparation method of amino acid bio-organic fertilizer, which is characterized in that including following preparation step:
S1. the preparation of fermenting agent
S1-1. the original strain of bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, Bacillus acidi lactici and aspergillus oryzae is sterile
Under the conditions of respectively successively through inclined-plane culture and liquid fermentation and culture；
S1-2. by the bacillus subtilis after liquid fermentation and culture, bacillus licheniformis, saccharomyces cerevisiae, Bacillus acidi lactici, aspergillus oryzae
Up to fermenting agent after mixing in proportion；
The preparation of S2 aminoacids solution
S2-1. meat mud is crushed with machine after Pomacea canaliculata being rinsed, by the volume mass of 1-5% than being added to after addition glucide
In fermentor, and carry out sterilization treatment；
S2-2. 0.5-5% fermenting agent as obtained by S1 is added into the fermentor after sterilization treatment, carries out fermented and cultured；
S2-2. fermentation liquid is transferred to plastics tank after fermentation, be placed at room temperature for, sealed fermenting 20-30 days up to amino acid
Liquid；
S3. the preparation of organic fertilizer
After decomposed fermentation by feces of livestock and poultry plus system chaff or dregs of rice class raw material to obtain the final product；
4) preparation of amino acid bio-organic fertilizer
The resulting aminoacids solution of S2 is added in the resulting organic fertilizer of S3 and carries out secondary fermentation, after fermentation up to amino acid
Biological organic fertilizer；Wherein, aminoacids solution is the 20%-60% of the organic fertilizer weight by envelope-bulk to weight ratio additive amount.
2. preparation method according to claim 1, which is characterized in that bacillus subtilis described in S1-1, lichens gemma
Bacillus, saccharomyces cerevisiae, Bacillus acidi lactici and the specific inclined-plane culture of aspergillus oryzae and liquid fermentation and culture step are as follows:
Bacillus subtilis, bacillus licheniformis original strain be aseptically seeded to LB after NA slant activation respectively
It condition of culture: ventilatory capacity 2.5kg, revolving speed 180r/min, 30 DEG C of cultivation temperature, cultivates to obtain the final product within fluid nutrient medium culture 18 hours
Time 18h；
The original strain of saccharomyces cerevisiae is aseptically seeded to bean sprouts medium culture after rose-bengal slant activation
28 hours to obtain the final product, condition of culture: ventilatory capacity 0kg, revolving speed 0r/min, 28 DEG C of cultivation temperature, incubation time 28h；
The original strain of Bacillus acidi lactici aseptically through MRS inclined plane inoculating to MRS fluid nutrient medium culture 28 hours to obtain the final product,
Condition of culture: ventilatory capacity 0kg, revolving speed 0r/min, 37 DEG C of cultivation temperature, incubation time 28h；
The original strain of aspergillus oryzae is aseptically seeded to bean sprouts medium culture 28 hours i.e. after PDA slant activation
, condition of culture: ventilatory capacity 2kg, revolving speed 120r/min, 28 DEG C of cultivation temperature, incubation time 28h.
3. preparation method according to claim 1, it is characterised in that: bacillus subtilis described in S1-2, lichens gemma
Bacillus, saccharomyces cerevisiae, Bacillus acidi lactici, aspergillus oryzae by volume 10:10:2:2:3 ratio mixing.
4. preparation method according to claim 1, it is characterised in that: glucide described in S2-1 is glucose or sugarcane
Sugar.
5. preparation method according to claim 1, it is characterised in that: fermentation culture conditions are revolving speed 225r/ in S2-2
Min, 28 DEG C of temperature, ventilatory capacity 0.5kg, ferment 36h.
6. preparation method according to claim 1, it is characterised in that: press 3% volume mass in muddy flesh described in S2-1
Than being added in fermentor after addition glucide；3% is added in S2-2 into the fermentor after sterilization treatment as obtained by S1
Fermenting agent carries out fermented and cultured；Fermentation time is 25 days in S2-3.
7. preparation method according to claim 1, which is characterized in that specific organic fertilizer preparation step includes: that will raise in S3
Poultry manure adds system chaff or dregs of rice class raw material to regulate carbon-nitrogen ratio and moisture, and 0.2% commercially available fermenting agent is added, sends out after mixing
Ferment, after fermentation 15-25 days to obtain the final product, wherein the commercially available effective bacterium of fermenting agent is bacillus subtilis, actinomyces, total effective
Viable count >=10,000,000,000/g.
8. preparation method according to claim 1, which is characterized in that secondary fermentation condition described in S4 are as follows: adjust fermentation
Heap moisture ferments 9-15 days to 40%-55%, when moisture be down to 30% hereinafter, to the end of fermentation.
9. a kind of amino acid bio-organic fertilizer, it is characterised in that: the amino acid bio-organic fertilizer is according to claim 1-7 method
It is prepared.
10. amino acid bio-organic fertilizer or claim 8 institute that a kind of any one of claim 1~7 the method is prepared
The application for the amino acid bio-organic fertilizer stated, it is characterised in that: be applied to crops；Preferably, the crops are capsicum；It is excellent
Choosing, amino acid bio-organic fertilizer usage amount 100-500kg per acre, it is furthermore preferred that usage amount is 250kg per acre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811331133.1A CN109160862A (en) | 2018-11-09 | 2018-11-09 | A kind of amino acid bio-organic fertilizer and the preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811331133.1A CN109160862A (en) | 2018-11-09 | 2018-11-09 | A kind of amino acid bio-organic fertilizer and the preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109160862A true CN109160862A (en) | 2019-01-08 |
Family
ID=64876866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811331133.1A Pending CN109160862A (en) | 2018-11-09 | 2018-11-09 | A kind of amino acid bio-organic fertilizer and the preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109160862A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111492741A (en) * | 2020-03-13 | 2020-08-07 | 广西壮族自治区农业科学院 | Method for improving soil improvement effect of organic fertilizer by using activated amino acid |
| CN112409057A (en) * | 2020-12-30 | 2021-02-26 | 赵兰坤 | Method for preparing organic fertilizer by using amino acid fermentation waste |
| CN115504841A (en) * | 2022-11-02 | 2022-12-23 | 安徽省农业科学院棉花研究所 | Fermented pig manure organic fertilizer and production method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000025844A (en) * | 1998-10-13 | 2000-05-06 | 이승욱 | Fermented fertilizer for agriculture and manufacturing method thereof using milk |
| CN103739314A (en) * | 2013-12-19 | 2014-04-23 | 江苏永禄肥料有限公司 | Preparation method of functional bacteria for fast and thorough decomposition of straws |
| CN104817369A (en) * | 2015-03-30 | 2015-08-05 | 苏州仁成生物科技有限公司 | Preparation method of biological organic fertilizer for improving fresh juice quality of Fuji apples |
| CN105061062A (en) * | 2015-08-13 | 2015-11-18 | 李志山 | Special-effect biological organic fertilizer and preparation method thereof |
| CN105418172A (en) * | 2016-01-11 | 2016-03-23 | 鹤壁市人元生物技术发展有限公司 | Preparation method of biological organic fertilizer |
| CN105543136A (en) * | 2016-01-11 | 2016-05-04 | 鹤壁市人元生物技术发展有限公司 | Bio-organic fertilizer fermented liquid microbial agent and preparing method thereof |
| CN107141120A (en) * | 2017-06-16 | 2017-09-08 | 上海禾绿生物有机肥有限公司 | A kind of special compound fertilizer of biological organic and preparation method thereof |
-
2018
- 2018-11-09 CN CN201811331133.1A patent/CN109160862A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000025844A (en) * | 1998-10-13 | 2000-05-06 | 이승욱 | Fermented fertilizer for agriculture and manufacturing method thereof using milk |
| CN103739314A (en) * | 2013-12-19 | 2014-04-23 | 江苏永禄肥料有限公司 | Preparation method of functional bacteria for fast and thorough decomposition of straws |
| CN104817369A (en) * | 2015-03-30 | 2015-08-05 | 苏州仁成生物科技有限公司 | Preparation method of biological organic fertilizer for improving fresh juice quality of Fuji apples |
| CN105061062A (en) * | 2015-08-13 | 2015-11-18 | 李志山 | Special-effect biological organic fertilizer and preparation method thereof |
| CN105418172A (en) * | 2016-01-11 | 2016-03-23 | 鹤壁市人元生物技术发展有限公司 | Preparation method of biological organic fertilizer |
| CN105543136A (en) * | 2016-01-11 | 2016-05-04 | 鹤壁市人元生物技术发展有限公司 | Bio-organic fertilizer fermented liquid microbial agent and preparing method thereof |
| CN107141120A (en) * | 2017-06-16 | 2017-09-08 | 上海禾绿生物有机肥有限公司 | A kind of special compound fertilizer of biological organic and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111492741A (en) * | 2020-03-13 | 2020-08-07 | 广西壮族自治区农业科学院 | Method for improving soil improvement effect of organic fertilizer by using activated amino acid |
| CN112409057A (en) * | 2020-12-30 | 2021-02-26 | 赵兰坤 | Method for preparing organic fertilizer by using amino acid fermentation waste |
| CN115504841A (en) * | 2022-11-02 | 2022-12-23 | 安徽省农业科学院棉花研究所 | Fermented pig manure organic fertilizer and production method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104529573B (en) | Many effect ferment fertilizer that a kind of agricultural wastes make | |
| CN102696458B (en) | Edible fungus compost, production method thereof and edible fungus culture process | |
| CN104496598B (en) | Special organic fertilizer for watermelons and preparation method of special organic fertilizer | |
| CN101126026B (en) | Vegetable field soil improving agent and its preparation method | |
| CN106386163A (en) | Method for cultivating edible fungi by using crop straw | |
| CN102442849A (en) | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method | |
| CN107580818B (en) | A kind of integrated approach of soil conditioning and reparation | |
| CN101372425A (en) | Microbial fertilizer and preparation thereof | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN107473786A (en) | A kind of method that rice straw returning to the field improves soil | |
| CN102249752A (en) | Microbial fertilizer and preparation method and application thereof | |
| CN103194410A (en) | Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same | |
| CN107285925A (en) | A kind of method of utilization microorganism formulation stalk stack retting returning to the field | |
| CN104692961A (en) | Microorganism organic material decomposition agent as well as preparation method and applications thereof | |
| CN107445694A (en) | A kind of composite bio-chemical preparation and its applied in straw-returning | |
| CN103159570A (en) | Solid microbial fertilizer and preparation method thereof | |
| CN109160862A (en) | A kind of amino acid bio-organic fertilizer and the preparation method and application thereof | |
| CN107298633A (en) | A kind of stalk stack retting biological agent | |
| CN105087421B (en) | The method that yeast fusion bacterium mixes microbial inoculum and preparation method thereof and production organic fertilizer | |
| CN103351188B (en) | Preparation method of agricultural organic fertilizer by treating pure straws as raw material | |
| CN103387431B (en) | Preparation method of pre-fermented chicken manure for agaricus bisporus culture medium | |
| CN105175165A (en) | Fertilizer special for continuous cropping resistance of roots of pilose asiabell | |
| CN105037042A (en) | Bio-fertilizer prepared through complex microbial inoculant secondary aerobic fermentation and preparation method thereof | |
| CN113121281A (en) | Rice seedling strengthening agent and preparation method thereof | |
| CN108640751A (en) | A kind of microbial manure, preparation method and application for preventing Radix Angelicae Sinensis root rot |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210406 Address after: Room 310-3, headquarters building, Changsha Zhongdian Software Park Co., Ltd., No. 39, Jianshan Road, Changsha hi tech Development Zone, Changsha, Hunan 410000 Applicant after: Hunan Huigu Agricultural Ecology Research Institute Co.,Ltd. Address before: 1504, 1505, luguyuyuan venture building, 27 Wenxuan Road, high tech Development Zone, Changsha, Hunan 410000 Applicant before: HUNAN TAIGU ECOLOGICAL ENGINEERING Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190108 |
|
| RJ01 | Rejection of invention patent application after publication |