CN105037042A - Bio-fertilizer prepared through complex microbial inoculant secondary aerobic fermentation and preparation method thereof - Google Patents
Bio-fertilizer prepared through complex microbial inoculant secondary aerobic fermentation and preparation method thereof Download PDFInfo
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Landscapes
- Fertilizers (AREA)
Abstract
The invention provides bio-fertilizer prepared through complex microbial inoculant secondary aerobic fermentation. The bio-fertilizer comprises, by weight, 300-330 parts of cornstalks, 280-340 parts of dried chicken manure, 13-16 parts of amino acid water solution, 40-48 parts of saccharose water solution, 25-30 parts of seepweed powder, 9-11 parts of wormwood, 5-7 parts of apatite, 3.8-4.4 parts of complex microbial inoculant A and 3.2-3.6 parts of complex microbial inoculant B. The complex microbial inoculant A comprises the microorganisms of pseudomonas stutzeri, bacillus stearothermophilus and vibrio proteolyticus. The complex microbial inoculant B comprises the microorganisms of trichoderma reesei, lactobacillus lactis, vibrio proteolyticus and monascus. The amino acid content of the prepared bio-fertilizer is 22-24%, the macromolecular protein content of the prepared bio-fertilizer is 19-23%, and the cellulose content of the prepared bio-fertilizer is reduced by 55-60%.
Description
Technical field
The invention belongs to Crop fertilizer field, particularly a kind ofly adopt bio-feritlizer of composite fungus agent secondary aerobic fermentation and preparation method thereof.
Background technology
Along with the development of economy and society and the foundation of intensive pasture, the generation of chicken manure grows with each passing day, according to statistics, Chinese feces of livestock and poultry generation tens tons in 2013, considerably beyond the total amount of industrial solid castoff then, feces of livestock and poultry has become the main source of city suburbs and rural environment, not only endangers ecotope and human health, also has influence on the Sustainable development of livestock and poultry industry.
From resource view, chicken manure, based on organic matter, containing a certain amount of N P and K and various medium trace element, through process, can become good agricultural fertilizer.
Rural area maize straw generation is huge simultaneously, and a large amount of maize straws is burned and abandons, and not only causes environmental pollution, and serious to soil organism waste.The elements such as the nitrogen contained in maize straw, phosphorus, potassium, magnesium, calcium, sulphur are the required main nutrient elements of crop growth, and therefore, maize straw is also abundant manure resources.Organic content average out to about 15% in maize straw, also containing nitrogen, phosphorus, quite a certain amount of carbon ammonium of potassium, calcium superphosphate and potassium sulfate etc.So maize straw has positive effect for improvement soil, sum up and mainly contain following 3 points: soil nutrient content 21, can be improved, soil property 3 can be improved, can farmland ecological environment be optimized.
Society develop actively bio-feritlizer and organic fertilizer, mostly be single bacterial classification or composite bacteria: a part is the mixture of microorganism and chemical fertilizer; Only having a small amount of is the complex body of the organism of maize straw class, composite fungus agent and feces of livestock and poultry.And using chicken manure and corn stalk powder as fermentation raw material, add composite fungus agent, inquire into optimal conditions of fermentation, Optimization Technology, reach and solve maize straw and chicken manure is innoxious and recycling object.
Make a general survey of the development trend of international bio fertilizer, be broadly divided into the following aspects:
1. developed to multi-functional aspect by single function, comprise the screening of associating flora, combination and use, fertilizer function is to fertilizer efficiency unification etc.
2. developed to composite biological fertilizer by Inoculant.Both can increase beneficial rhizosphere flora, active soil organic matter and nutritive element can be increased again, and make fertilizer for efficient bacterium propagation and activity and base is provided.
3. effect is improved.
4. with nutritive element or organic reasonable compound.
5. with PGPR(roots of plants circle growth-promoting bacterium) combined utilization.
The chicken manure of prior art and the bio-feritlizer of maize straw fermentative production have following defect: (1) aminoacids content is low, high molecular weight protein and content of cellulose high, plant be not easy absorb and utilize; (2) use the fermenting organism fertilizer of prior art, improve crop yield effect poor; (3) the fermenting organism fertilizer of prior art, improves growing area soil DeGrain.
Summary of the invention
The present invention is directed to above deficiency, provide a kind of and adopt bio-feritlizer of composite fungus agent secondary aerobic fermentation and preparation method thereof, realize following goal of the invention:
1, improve the aminoacids content of bio-feritlizer, reduce high molecular weight protein content and content of cellulose; Make aminoacids content be 22-24%, high molecular weight protein content is 19-23%, and content of cellulose reduces 55-60%;
2, adopt bio-feritlizer of the present invention, improve crop-planting output;
3, adopt bio-feritlizer of the present invention, improve the quality of long-term cropping;
4, adopt bio-feritlizer of the present invention, obviously improve growing area soil, improve water capacity, porosity, nutritive element content.
for solving the problems of the technologies described above, by the following technical solutions:
Adopt a bio-feritlizer for composite fungus agent secondary aerobic fermentation, the raw material of described bio-feritlizer comprises the component of following weight proportion: maize straw 300-330 part, cicken feces dried 280-340 part, amino acid solution 13-16 part, aqueous sucrose solution 40-48 part, alkali fluffy powder 25-30 part, wormwood 9-11 part, phosphatic rock 5-7 part, composite fungus agent A3.8-4.4 part, composite fungus agent B3.2-3.6 part.
Below the further improvement to technique scheme:
Described composite fungus agent A comprises the microorganism of following content: Pseudomonas stutzeri 20 × 10
9cFU/g, bacstearothermophilus 10 × 10
9cFU/g, vibrio proteolyticus 0.5 × 10
9cFU/g.
Described composite fungus agent B comprises the microorganism of following content: Trichodermareesei 13 × 10
9cFU/g, De Shi lactobacillus lactis 15 × 10
9cFU/g, vibrio proteolyticus 1.5 × 10
9cFU/g, monascus 15 × 10
9cFU/g.
The raw material of described bio-feritlizer comprises the component of following weight proportion: maize straw 310 parts, cicken feces dried 300 parts, amino acid solution 14.5 parts, aqueous sucrose solution 44 parts, 27 parts, the fluffy powder of alkali, wormwood 10 parts, 6 parts, phosphatic rock, composite fungus agent A4.1 part, composite fungus agent B3.4 part.
Described preparation method prepares composite fungus agent A, prepares composite fungus agent B, the preparation of the base-material that becomes thoroughly decomposed, first time fermentation, second time fermentation step.
It is the disintegrating slag of 0.2-1cm that the preparation step of the described base-material that becomes thoroughly decomposed comprises corn straw smashing; Ground by chicken manure, fineness is 40 orders.
Described first time fermentation step: in the base-material that becomes thoroughly decomposed, be sprinkled into composite fungus agent A, be evenly sprinkled into, 80rpm stirs 20min, piles the heap that becomes thoroughly decomposed of highly about 1.5 meters, and fermentation, turns over throwing once in every five days, ferment 20 days.
Described second time fermentation step: be sprinkled into composite fungus agent B to the base-material that becomes thoroughly decomposed, be evenly sprinkled into, 100rpm stirs 10min, piles the heap that becomes thoroughly decomposed of highly about 1 meter, carries out normal temperature ageing fermentation, within every 1 day, turns over throwing once, ferments 10 days.
Adopt bio-feritlizer prepared by the present invention, aminoacids content is 22-24%, and high molecular weight protein content is 19-23%, and content of cellulose reduces 55-60%.
compared with prior art the present invention has following beneficial effect:
1, the bio-feritlizer adopting the present invention to prepare, aminoacids content is 22-24%, and high molecular weight protein content is 19-23%, and content of cellulose reduces 55-60%;
2, bio-feritlizer nutritive element of the present invention is complete, is easily absorbed by plants, and significantly improves crop-planting output; Adopt bio-feritlizer of the present invention to plant Radix Dauci Sativae, output is 6500-6800kg/ mu;
3, bio-feritlizer of the present invention is adopted to significantly improve the quality of long-term cropping; Adopt the Radix Dauci Sativae that bio-feritlizer of the present invention is planted: protein content is 0.68-0.72g/100g, calcium contents is 42-45mg/100g, and carotene carotene content is 3.82-3.86mg/100g;
4, bio-feritlizer of the present invention improves significantly to soil, adopt bio-feritlizer of the present invention after 1 year, growing area soil is had clear improvement, water capacity improves 9-12%, soil porosity improves 12.8-15.4%, soil total N content improves 22.1-24.6%, and available potassium in soils content improves 43.2-45.6%.
Embodiment
Below in conjunction with embodiment, the present invention is described further.
In the present invention, Pseudomonas stutzeri (
pseudomonasstutzeri) preserving number is ACCC01977, bacstearothermophilus (
bacillusstearothermophilus) preserving number is CGMCC1.1923, vibrio proteolyticus (
vibrioproteolyticus) preserving number is MCCC1H00046, Trichodermareesei (
trichodermareesei) preserving number be ACCC30911, De Shi lactobacillus lactis (
lactobacillusdelbrueckii) preserving number is ACCC10183, monascus (
monascusanka) preserving number is ACCC30341, above-mentioned bacterial classification is all from commercially available.
embodiment 1 one kinds adopts the bio-feritlizer of composite fungus agent secondary aerobic fermentation
The raw material of described bio-feritlizer comprises the component of following weight proportion:
Maize straw 300 parts, cicken feces dried 280 parts, amino acid solution 16 parts, aqueous sucrose solution 40 parts, 25 parts, the fluffy powder of alkali, wormwood 9 parts, 5 parts, phosphatic rock, composite fungus agent A4.4 part, composite fungus agent B3.2 part;
Described cicken feces dried: water content is 13%;
Described amino acid solution: containing leucine 3%, Isoleucine 7%, α-amino-isovaleric acid 6%, Gelucystine 5%, methionine(Met) 9%;
Described aqueous sucrose solution: concentration is 60%;
Described wormwood: be fineness 80 object powder, water content is 7%;
Described composite fungus agent A comprises the microorganism of following content: Pseudomonas stutzeri 20 × 10
9cFU/g, bacstearothermophilus 10 × 10
9cFU/g, vibrio proteolyticus 0.5 × 10
9cFU/g;
Described composite fungus agent B comprises the microorganism of following content: Trichodermareesei 13 × 10
9cFU/g, De Shi lactobacillus lactis 15 × 10
9cFU/g, vibrio proteolyticus 1.5 × 10
9cFU/g, monascus 15 × 10
9cFU/g.
Above-mentioned percentage ratio is mass percent.
embodiment 2 one kinds adopts the bio-feritlizer of composite fungus agent secondary aerobic fermentation
The raw material of described bio-feritlizer comprises the component of following weight proportion:
Maize straw 310 parts, cicken feces dried 300 parts, amino acid solution 14.5 parts, aqueous sucrose solution 44 parts, 27 parts, the fluffy powder of alkali, wormwood 10 parts, 6 parts, phosphatic rock, composite fungus agent A4.1 part, composite fungus agent B3.4 part;
Described cicken feces dried: water content is 13%;
Described amino acid solution: containing leucine 3%, Isoleucine 7%, α-amino-isovaleric acid 6%, Gelucystine 5%, methionine(Met) 9%;
Described aqueous sucrose solution: concentration is 60%;
Described wormwood: be fineness 80 object powder, water content is 7%;
Described composite fungus agent A comprises the microorganism of following content: Pseudomonas stutzeri 20 × 10
9cFU/g, bacstearothermophilus 10 × 10
9cFU/g, vibrio proteolyticus 0.5 × 10
9cFU/g;
Described composite fungus agent B comprises the microorganism of following content: Trichodermareesei 13 × 10
9cFU/g, De Shi lactobacillus lactis 15 × 10
9cFU/g, vibrio proteolyticus 1.5 × 10
9cFU/g, monascus 15 × 10
9cFU/g.
Above-mentioned percentage ratio is mass percent.
embodiment 3 one kinds adopts the bio-feritlizer of composite fungus agent secondary aerobic fermentation
The raw material of described bio-feritlizer comprises the component of following weight proportion:
Maize straw 330 parts, cicken feces dried 340 parts, amino acid solution 13 parts, aqueous sucrose solution 48 parts, 30 parts, the fluffy powder of alkali, wormwood 11 parts, 7 parts, phosphatic rock, composite fungus agent A3.8 part, composite fungus agent B3.6 part;
Described cicken feces dried: water content is 13%;
Described amino acid solution: containing leucine 3%, Isoleucine 7%, α-amino-isovaleric acid 6%, Gelucystine 5%, methionine(Met) 9%;
Described aqueous sucrose solution: concentration is 60%;
Described wormwood: be fineness 80 object powder, water content is 7%;
Described composite fungus agent A comprises the microorganism of following content: Pseudomonas stutzeri 20 × 10
9cFU/g, bacstearothermophilus 10 × 10
9cFU/g, vibrio proteolyticus 0.5 × 10
9cFU/g;
Described composite fungus agent B comprises the microorganism of following content: Trichodermareesei 13 × 10
9cFU/g, De Shi lactobacillus lactis 15 × 10
9cFU/g, vibrio proteolyticus 1.5 × 10
9cFU/g, monascus 15 × 10
9cFU/g.
Above-mentioned percentage ratio is mass percent.
embodiment 4 one kinds adopts the preparation method of the bio-feritlizer of composite fungus agent secondary aerobic fermentation
Step 1, prepare composite fungus agent A and composite fungus agent B
A. composite fungus agent A is prepared:
Respectively by Pseudomonas stutzeri, bacstearothermophilus and vibrio proteolyticus; Carry out actication of culture, spread cultivation, nutrient solution, through centrifugal, isolate slag, obtains bacteria suspension; Bacteria suspension mixes and is sprayed on carrier, the composite fungus agent A of obtained pulvis;
Described composite fungus agent A water content is 28%;
Described carrier is the mixture of starch and white sugar, and mixed weight is than being 3:0.5;
In described composite fungus agent A, content of microorganisms is: Pseudomonas stutzeri 20 × 10
9cFU/g, bacstearothermophilus 10 × 10
9cFU/g, vibrio proteolyticus 0.5 × 10
9cFU/g;
B. composite fungus agent B is prepared:
Respectively by Trichodermareesei, De Shi lactobacillus lactis, vibrio proteolyticus and monascus; Carry out actication of culture, spread cultivation, nutrient solution, through centrifugal, isolate slag, obtains bacteria suspension; Bacteria suspension mixes and is sprayed on carrier, the composite fungus agent B of obtained pulvis;
Described composite fungus agent B water content is 34%;
Described carrier is starch;
In described composite fungus agent B, content of microorganisms is: Trichodermareesei 13 × 10
9cFU/g, De Shi lactobacillus lactis 15 × 10
9cFU/g, vibrio proteolyticus 1.5 × 10
9cFU/g, monascus 15 × 10
9cFU/g.
The preparation of step 2, the base-material that becomes thoroughly decomposed
A. be the disintegrating slag of 0.2-1cm by corn straw smashing; Ground by chicken manure, fineness is 40 orders;
B. each raw material is taken by formula, for subsequent use;
C. by maize straw, cicken feces dried, the fluffy powder of alkali, wormwood, apatites mixed, 60rpm stirs, and sprays into amino acid solution and aqueous sucrose solution, obtain the base-material that becomes thoroughly decomposed in whipping process.
Step 3, first time fermentation
In the ready base-material that becomes thoroughly decomposed of step (2), be sprinkled into composite fungus agent A, be evenly sprinkled into, 80rpm stirs 20min, piles the heap that becomes thoroughly decomposed of highly about 1.5 meters, and fermentation, turns over throwing once in every five days, ferment 20 days.
Step 4, second time fermentation
For the first time after fermentation ends, the heap that will become thoroughly decomposed is spread out, dries in the air to normal temperature; Be sprinkled into composite fungus agent B, be evenly sprinkled into, 100rpm stirs 10min, piles the heap that becomes thoroughly decomposed of highly about 1 meter, carries out normal temperature ageing fermentation, within every 1 day, turns over throwing once, ferments 10 days, obtains refined biometric fertilizer.
result detects:
(1) bio-feritlizer of the present invention is detected, the results are shown in Table 1;
The bio-feritlizer detected result of table 1 embodiment 1-3:
From table 1, adopt the present invention to prepare bio-feritlizer, significantly improve the aminoacids content of fertilizer, reduce high molecular weight protein content and content of cellulose, aminoacids content is 22-24%, and high molecular weight protein content is 19-23%, and content of cellulose reduces 55-60%.
(2) bio-feritlizer of embodiment 1-3 is used for the plantation of Radix Dauci Sativae as base manure, and with common brid guano and maize straw fertilizer for control group, sowing amount is 200kg/ mu; Except the base manure used, other implantation methods are all identical, and the edaphic condition of plantation field is also identical, detects after plantation to planting experiment result; Result is as shown in table 1:
The bio-feritlizer of table 2 embodiment 1-3 is used for the effect of growth of carrot
From table 2, adopt bio-feritlizer of the present invention to plant Radix Dauci Sativae, output is the Radix Dauci Sativae of 6500-6800kg/ mu, plantation: protein content is 0.68-0.72g/100g, and calcium contents is 42-45mg/100g, and carotene carotene content is 3.82-3.86mg/100g.
(2) adopt the bio-feritlizer of embodiment 1-3 after 1 year, detect plantation field soil, result is as shown in table 2:
Table 3 adopts the soil root system of bio-feritlizer after 1 year of embodiment 1-3
From table 3, adopt bio-feritlizer of the present invention after 1 year, obvious improvement is obtained to growing area soil, water capacity improves 9-12%, soil porosity improves 12.8-15.4%, and soil total N content improves 22.1-24.6%, and available potassium in soils content improves 43.2-45.6%.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. adopt a bio-feritlizer for composite fungus agent secondary aerobic fermentation, it is characterized in that: the raw material of described bio-feritlizer comprises the component of following weight proportion: maize straw 300-330 part, cicken feces dried 280-340 part, amino acid solution 13-16 part, aqueous sucrose solution 40-48 part, alkali fluffy powder 25-30 part, wormwood 9-11 part, phosphatic rock 5-7 part, composite fungus agent A3.8-4.4 part, composite fungus agent B3.2-3.6 part.
2. a kind of bio-feritlizer adopting composite fungus agent secondary aerobic fermentation according to claim 1, is characterized in that: described composite fungus agent A comprises the microorganism of following content: Pseudomonas stutzeri 20 × 10
9cFU/g, bacstearothermophilus 10 × 10
9cFU/g, vibrio proteolyticus 0.5 × 10
9cFU/g.
3. a kind of bio-feritlizer adopting composite fungus agent secondary aerobic fermentation according to claim 1, is characterized in that: described composite fungus agent B comprises the microorganism of following content: Trichodermareesei 13 × 10
9cFU/g, De Shi lactobacillus lactis 15 × 10
9cFU/g, vibrio proteolyticus 1.5 × 10
9cFU/g, monascus 15 × 10
9cFU/g.
4. a kind of bio-feritlizer adopting composite fungus agent secondary aerobic fermentation according to claim 1, is characterized in that: the raw material of described bio-feritlizer comprises the component of following weight proportion: maize straw 310 parts, cicken feces dried 300 parts, amino acid solution 14.5 parts, aqueous sucrose solution 44 parts, 27 parts, the fluffy powder of alkali, wormwood 10 parts, 6 parts, phosphatic rock, composite fungus agent A4.1 part, composite fungus agent B3.4 part.
5. adopt a preparation method for the bio-feritlizer of composite fungus agent secondary aerobic fermentation, it is characterized in that: described preparation method prepares composite fungus agent A, prepares composite fungus agent B, the preparation of the base-material that becomes thoroughly decomposed, first time fermentation, second time fermentation step.
6. a kind of preparation method adopting the bio-feritlizer of composite fungus agent secondary aerobic fermentation according to claim 5, is characterized in that: described in become thoroughly decomposed that the preparation step of base-material comprises corn straw smashing be the disintegrating slag of 0.2-1cm; Ground by chicken manure, fineness is 40 orders.
7. a kind of preparation method adopting the bio-feritlizer of composite fungus agent secondary aerobic fermentation according to claim 5, it is characterized in that: described first time fermentation step: in the base-material that becomes thoroughly decomposed, be sprinkled into composite fungus agent A, evenly be sprinkled into, 80rpm stirs 20min, pile the heap that becomes thoroughly decomposed of highly about 1.5 meters, fermentation, turns over throwing once in every five days, ferments 20 days.
8. a kind of preparation method adopting the bio-feritlizer of composite fungus agent secondary aerobic fermentation according to claim 5, it is characterized in that: described second time fermentation step: be sprinkled into composite fungus agent B to the base-material that becomes thoroughly decomposed, evenly be sprinkled into, 100rpm stirs 10min, pile the heap that becomes thoroughly decomposed of highly about 1 meter, carry out normal temperature ageing fermentation, within every 1 day, turn over throwing once, ferment 10 days.
9. a kind of preparation method adopting the bio-feritlizer of composite fungus agent secondary aerobic fermentation according to claim 5, it is characterized in that: adopt bio-feritlizer prepared by the present invention, aminoacids content is 22-24%, and high molecular weight protein content is 19-23%, and content of cellulose reduces 55-60%.
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| CN107473867A (en) * | 2017-10-09 | 2017-12-15 | 山东沃科奇实业有限公司 | Biological bacterial fertilizer prepared from livestock and poultry waste and preparation method thereof |
| CN107857689A (en) * | 2017-12-28 | 2018-03-30 | 青岛嘉瑞生物技术有限公司 | The technique that a kind of bioanalysis prepares bio-organic fertilizer using chicken manure |
| CN109020758A (en) * | 2018-10-11 | 2018-12-18 | 湖北文理学院 | A kind of base manure and preparation method thereof suitable for Xiangyang yam planting |
| CN113620752A (en) * | 2021-09-06 | 2021-11-09 | 广东汇先丰农牧有限公司 | Chicken manure organic fertilizer rich in microbial strains and preparation method thereof |
| CN113956088A (en) * | 2021-11-29 | 2022-01-21 | 江苏农乐生物科技有限公司 | Fertilizer containing xylo-oligosaccharide and application of fertilizer to promoting watermelon growth |
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| CN107857689A (en) * | 2017-12-28 | 2018-03-30 | 青岛嘉瑞生物技术有限公司 | The technique that a kind of bioanalysis prepares bio-organic fertilizer using chicken manure |
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