CN106047764A - Low-temperature beneficial microorganism preparation product applied to straw application - Google Patents
Low-temperature beneficial microorganism preparation product applied to straw application Download PDFInfo
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- CN106047764A CN106047764A CN201610586110.XA CN201610586110A CN106047764A CN 106047764 A CN106047764 A CN 106047764A CN 201610586110 A CN201610586110 A CN 201610586110A CN 106047764 A CN106047764 A CN 106047764A
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- volcanic rock
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- bacillus subtilis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to a low-temperature beneficial microorganism preparation product applied to straw application. The low-temperature beneficial microorganism preparation product comprises a microbial agent, a volcanic biological agent and amino acid particles, wherein the microbial agent comprises a plurality of beneficial microorganisms using bacillus subtilis KX-1, bacillus subtilis KX-2, bacillus subtilis KX-4 and actinomycetes as the main bodies; the volcanic biological agent comprises a volcanic particle carrier and a microbial agent implanted into the volcanic particle carrier. The low-temperature beneficial microorganism preparation product has the advantages that the product uses low-temperature bacteria as the induction bacteria, is high in activity, capable of reproducing under a low-temperature condition and driving the high-speed reproduction of other microbial agents, capable of achieving both high-temperature fermentation and low-temperature fermentation, unlimited by regions and seasons and capable of being used in four seasons; the product is applicable to straw application, farmers can perform straw application right in the field, plant ash organic fertilizer can be formed, straw application in a harmless fertilizer form is achieved, beneficial microorganisms in soil are increased, and the fertility of the soil is increased.
Description
Technical field
The present invention relates to a kind of low temperature effective microorganism preparation product applying to straw-returning.
Background technology
China's stalk resource is the abundantest, but fails rational exploitation and utilization in producing, and not only wastes organic resource, also makes
Become serious environmental problem.In July, 2008, State Council issued " about the meaning accelerating to advance agricultural crop straw comprehensive utilization
See ".This is for accelerating to advance stalk comprehensive utilization, it is achieved the resource of straw, commercialization, promotes resources conservation, environmental conservation
With increasing peasant income, there is important function.2015, State Development and Reform Commission of state, the Ministry of Finance, the Ministry of Agriculture, Environmental Protection Department were issued jointly logical
Know, it is desirable to various places are strengthened stalk comprehensive utilization further and burnt work with prohibiting, and strive to the year two thousand twenty whole nation stalk comprehensive utilization rate reaching
To more than 85%.
Straw-returning is that the straw (wheat straw, corn stalk and rice straw etc.) that should not directly make feedstuff directly or is piled up corruption
A kind of method in being manured into soil after ripe, is the well stimulation of a culture fertility of most attention in the world today, is stopping
Weight gaining production-increasing function is also had while the atmospheric pollution that crop straw burning is caused.Straw-returning can be promoted agricultural water conservation, joint
Cost, volume increase, potentiation, fully paid attention in environmental protection and agricultural sustainable development.
It is known that stalk resource is one of agricultural by-products that in the Northeast's agricultural production, quantity is bigger, rice straw and
The substantial amounts of wheat straw, but the nutritive value as feedstuff is the highest, tackles it and comprehensively recycles, with innoxious fertilizer form also
Field.But, the Northeast Oryza sativa L. is only planted a season, and the general annual October can gather in, and area, the Three Eastern Provinces daytime of October
Night, the temperature difference was the biggest.The microniological proudcts for straw-returning in the market, are affected by southern and northern seasonal climate temperature, are used for
Temperature lower ground district's weak effect.Under low temperature state, microorganism is difficult to growth and breeding, makes heap temperature rise, it is impossible at low temperature shape
For straw-returning under state.Furthermore large-scale industrialized straw-returning does not has operability because of transport, place, Cost Problems.Cause
This, for northern China cold district, low autumn, straw directly returning to field can not become thoroughly decomposed in time, affect this season plant growth be
Unavoidable.
Authorization Notice No. CN102174424B discloses the microbial bacterial agent of a kind of efficient degradation crop material, and it is by lichens
2 kinds of bacterial strain compositions such as bacillus cereus, Deng Shitaiteng bacillus cereus Wei, wherein think that this microbial inoculum can be used for the straw-returning in the north,
But it is only limitted under indoor higher temperature operate, it is impossible to realize solving on the spot straw-returning or need when spending very long
Between.
Summary of the invention
The technical problem to be solved is to overcome the deficiencies in the prior art, it is provided that one can wait cold northeastward
Area carries out the low temperature effective microorganism preparation product of straw-returning.
For solving above technical problem, the present invention adopts the following technical scheme that
A kind of method that it is an object of the present invention to provide straw-returning, is carried out straw including utilizing microbial bacterial agent
The step of decomposition reactor system, described microbial bacterial agent include bacillus subtilis KX-1 that preserving number is CCTCC NoM208057,
Actinomycetes, preserving number are the bacillus subtilis KX-2 of CCTCC NoM208058, preserving number is the withered of CCTCC NoM208060
Grass bacillus cereus KX-4.
Preferably, described bacillus subtilis KX-1, described actinomycetes, described bacillus subtilis KX-2, institute
The consumption mass ratio of the bacillus subtilis KX-4 stated is 1:0.3~0.75:0.4~0.85:0.5~0.85, more preferably 1:
0.6~0.7:0.7~0.8:0.7~0.8.
Preferably, described microbial bacterial agent also includes other beneficial microbes, and other described beneficial microbes are choosing
One or more in lactic acid bacteria, photosynthetic bacteria, yeast, Bacillus licheniformis.
It is further preferred that described bacillus subtilis KX-1 and other described beneficial microorganism consumption mass ratioes
For 1:0.1~0.8, more preferably 1:0.5~0.8.
Preferably, described microbial bacterial agent also includes the enzyme preparation that selectively adds so that described microbial bacterial agent
Acid protease >=2000u/g, neutral protease >=10000u/g, cellulase >=10000u/g.
Wherein, enzyme preparation is acid protease, neutral protease, cellulase, when certain in described microbial bacterial agent
When planting enzyme deficiency, i.e. add corresponding enzyme preparation.
Mode according to one embodiment of present invention, first activates described microbial bacterial agent, then with nutrient
After plasmogamy is set to mixed liquor, mix with dry straw, straw is carried out decomposition reactor system.
Preferably, described microbial bacterial agent is 1:1000~2000 with the consumption mass ratio that feeds intake of described dry straw.
Mode according to another embodiment of the invention, first activates described microbial bacterial agent, then with nutrition
Material is configured to mixed liquor, then described mixed liquor, volcanic rock biological preparation is mixed with dry Fructus Citri tangerinae bar, straw is carried out composting
Becoming thoroughly decomposed, described volcanic rock biological preparation includes volcanic rock particulate vector, the micro-life being implanted on described volcanic rock particulate vector
Thing microbial inoculum.
Preferably, described microbial bacterial agent, described volcanic rock biological preparation, the consumption matter that feeds intake of described dry straw
Amount ratio is 1:50~80:1000~2000.
The present invention use conventional method carry out Stain activation.
Preferably, described nutrient substance is carbamide and amino acid granular.
Preferably, carry out fermentation reactor system when becoming thoroughly decomposed, control humidity 50~65%.
More specifically, during composting, assign permeability cell, then cover film on composting.
Further, when carrying out fermentation maturity, treat that the temperature of composting rises to 50~60 DEG C, remove and cover above permeability cell
Thin film continue fermentation 10~15 days, then carry out turning moisturizing, continue fermentation and complete fermentation maturity in 5~15 days.
After fermentation maturity terminates, plough under field when can plough under field as required or continue composting to Rice Cropping in the coming year and make
Use for bio-bacterial manure, be administered in farmland.Soil microbial activity can be promoted, improve soil organic matter content, at certain model
Enclose the dynamic equilibrium of interior maintenance soil with organic matter, improve the physical arrangement of soil, fixing and holding soil nitrogen nutriment, increase
The effectiveness of difficultly soluble nutrient, recovers and maintenance of soil fertility, solves the farmland quality owing to farming causes year after year and declines, soil
Organic not enough, the problems such as soil water retention, fertilizer conservation, venting capability reduce, soil aggregate minimizing, it is greatly facilitated agricultural raw
Produce.
It is a further object to provide a kind of low temperature effective microorganism preparation product, including microbial bacterial agent, institute
The microbial bacterial agent stated includes that bacillus subtilis KX-1 that preserving number is CCTCC NoM208057, actinomycetes, preserving number are
The bacillus subtilis KX-2 of CCTCC NoM208058, preserving number are the bacillus subtilis KX-4 of CCTCC NoM208060.
Preferably, described bacillus subtilis KX-1, described actinomycetes, described bacillus subtilis KX-2, institute
The consumption mass ratio of the bacillus subtilis KX-4 stated is 1:0.3~0.75:0.4~0.85:0.5~0.85, more preferably 1:
0.6~0.7:0.7~0.8:0.7~0.8.
Wherein, preserving number be the bacillus subtilis KX-1 of CCTCC NoM208057 be Bacillus subtilis natto, for low
Temperature bacterium, can be 2~8 DEG C of breeding survivals.
Preferably, described microbial bacterial agent also includes other beneficial microbes, and other described beneficial microbes are choosing
One or more in lactic acid bacteria, photosynthetic bacteria, yeast, Bacillus licheniformis.
It is further preferred that described bacillus subtilis KX-1 and other described beneficial microorganism consumption mass ratioes
For 1:0.1~0.8, more preferably 1:0.5~0.8.
Preferably, described microbial bacterial agent also includes the enzyme preparation that selectively adds so that described microbial bacterial agent
Acid protease >=2000u/g, neutral protease >=10000u/g, cellulase >=10000u/g.
In the present invention, microbial bacterial agent can be the mixture of as above many kinds of substance;Can also be that many kinds of substance individually wraps
Dress, is carried out mixing by proportioning during use.Preferably, microbial bacterial agent is can be the mixture of as above many kinds of substance.
Preferably, described low temperature effective microorganism preparation product also includes volcanic rock biological preparation, described volcanic rock
Biological preparation includes volcanic rock particulate vector, the microbial bacterial agent being implanted on described volcanic rock particulate vector.
Preferably, the present invention implants the formula of microbial bacterial agent on described volcanic rock particulate vector and above-mentioned micro-life
Thing microbial inoculum is identical.
Specifically, the preparation method of described volcanic rock biological preparation comprises the steps:
Step (1), volcanic rock is milled to the volcanic rock granule of 20~40 mesh, obtains volcanic rock granule after removing powder and carry
Body;
Step (2), it is that 1:20~50 is configured to bacterium solution, then by described microbial bacterial agent and water according to consumption mass ratio
Add nitrogen source and strain is activated by carbon source, when the bacillus cereus more than 80% in bacterium solution is in trophosome state, viable count >
During 5000000000/mL, described volcanic rock particulate vector is soaked in described bacterium solution 24~72h, then by described volcanic rock
Particulate vector is pulled out and is drained, and obtains described volcanic rock biological preparation.
The nitrogen source used in the present invention and carbon source are conventional nitrogen source and carbon source, such as: Testa oryzae, brown sugar, glucose or sugar
Slurry.
Preferably, described low temperature effective microorganism preparation product also includes amino acid granular.
Microbial bacterial agent, volcanic rock biological preparation and amino acid granular in the present invention use independent packaging.
Due to the enforcement of technique scheme, the present invention compared with prior art has the advantage that
The present invention is with pyschrophile for induction bacterium, and it contains hay (containing natto) bacillus cereus, actinomycetes are the multiple of main body
Complex function beneficial microbe, it is possible to breed in the case of low temperature, drive the high speed of other microbial inoculum to breed.Can send out by high temperature
Ferment, again can low temperature fermentation, not by area, season limited, the four seasons can be used, and peasant can be processed nearby at the edge of a field, solves on the spot
Straw-returning, forms plant ash organic fertilizer, with innoxious fertilizer form also field, it is not necessary to straw is carried, saving manpower,
Material resources, financial resources;The present invention contains amino acid granular further, it is possible to the addition of nitrogen source (such as carbamide) when reducing compost, for micro-
Biological breeding provides nutrition;The volcanic rock that the present invention also abounds with further combined with locality, Jilin, utilizes the porous of volcanic rock, table
The architectural characteristic that area is big, play its water conservation, can as the carrier of microbial inoculum and strengthen heat sinking function effect, and solve work as
The problem of the utilization of the ground natural mineral resources of this part;The present invention do not comprises trichodermin bacterium, it is to avoid to the injury behind also field, excellent
Select the fiber that the microorganism that can produce exoenzyme (mainly lignin oxidation's enzyme system, cellulase etc.) comes in degrading straw
Element, hemicellulose, lignin, effect of accelerating to become thoroughly decomposed.
Figure of description
Fig. 1 is the picture of straw before and after fermentation;
Fig. 2 is the picture of the Fructus Citri tangerinae bar fermentation heap with steam vent;
Fig. 3 is the picture of the good stalk fermentation heap of degree of becoming thoroughly decomposed;
Fig. 4 is the picture of secondary turning moisturizing.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described in detail, but the present invention is not limited to following enforcement
Example.
Embodiment 1:
One, main component
1, microbial bacterial agent, by weight
The composite bacteria agent capable prepared requires: acid protease >=2000u/g, neutral protease >=10000u/g, cellulose
Enzyme >=10000u/g, insufficient section adds corresponding enzyme preparation.
Bacillus subtilis KX-1 can be at 2~8 DEG C of survival and reproductions.
2, volcanic rock biological preparation
Volcanic rock biological preparation, is the architectural characteristic utilizing the porous of volcanic rock, surface area big, containing substantial amounts of silicon, potassium,
Tens kinds of mineral and the trace element such as sodium, ferrum, magnesium, aluminum, silicon, calcium, titanium, manganese, ferrum, nickel, cobalt, and the chelating of heavy metal
Property, with it as carrier, implant through checking, the multiple beneficial microorganism species that people and animals and other biological are harmless (is comprised unwrapping wire
The multiple beneficial microorganism such as bacterium, bacillus cereus, based on pyschrophile) product that formed afterwards.Volcano biological preparation is at composting process
There is the effect of microorganism enrichment slow release and effect that heat radiation is ventilative, additionally can promote that soil granular is tied with water conservation, water-retention
The formation of structure, is effectively improved plant root microbial environment, provides trace element for plant growth, promotes plant growing.
Concrete preparation method:
A, the preparation of base material
After natural volcanic rock is milled to the granule less than 1mm, removal powder, stand-by;
B, the preparation of bacterium solution
And prepare composite bacteria agent capable according to formula as below: by weight
1. activate
The compounding beneficial functions bacterium chosen is inserted there is the clean square groove into water, aeration, heated for controlling temperature and drain function,
According to 1:20~50 dilution, add a certain amount of nitrogen source, carbon source, activate strain;
2. soak
Under microscope, microscopy bacillus cereus is mostly in trophosome state, and plate colony count detection viable count > 5,000,000,000/
ML, can put into square groove by the carrier after processing and soak;
3. draining, drain
After soaking 24~72h, by bacterium solution after the end drains, carrier is pulled out and drains;
3, amino acid granular
Pure biofermentation concentrates and prepares, containing abundant nitrogen source and other nutritional labelings.
Two, the application in straw-returning
(1), relevant device and material
1, large-scale fodder crushing cutting machine: the Caulis et Folium Oryzae dried is cut into 1~2cm length.
2, large-scale blender: the large-scale mortar mixer of removing from mould after advance.
3, large-scale aerosol apparatus: spray microbial inoculum and the mixed liquor of nutritional solution when spice.
4, agricultural plastic film: some.
5, Stain activation equipment.
6, spice related tool or equipment.
7, permeability cell: diameter about 10cm, the around plastic tube of punching.
8, other material: brown sugar, Testa oryzae, carbamide
(2), usage, consumption and fermentation period
1, consumption
Every 1 kilogram of microbial bacterial agent+60kg volcanic rock biological preparation, about ferments, become thoroughly decomposed 1~2 ton of dry straw, fermentation, rotten
Ambient temperature time ripe is more than 5~10 DEG C.
2, usage (to activate 1Kg strain, as a example by fermentation 1T straw)
1) Stain activation
With water according to the dilution proportion of 1:20~30, add fresh for 4Kg Testa oryzae, 0.5Kg brown sugar activates 12h.
2) nutrient source and auxiliaries
Carbamide 22Kg, amino acid granular 10Kg, after fully dissolving with water, be diluted to about 1.2~1.5T.
3) mixing
By 1) in activate after bacterium solution add 2) in be mixed into mixed liquor.
4) spice: humid control is 50~about 65%
Straw after pulverizing is sent into from blender front end (charging aperture), and after adding volcano biological preparation, uses from two ends
Aerosol apparatus sprays 3 to charging aperture and discharging opening simultaneously) in mixed liquor, after stirring from blender rear end export, carry out heap
System.
5) heap built by material
Heap makes pyramid type (base diameter is about 3 meters) or strip ladder type (bottom width 2m, high 1.5m, upper wide 1.0m), suitable
Depending on practical situation, and place permeability cell, cover film, ferments.When after 3 days, temperature is up to 50~60 DEG C, will be ventilative
The thin film covered on pipe removes.
6) secondary turning
After heap restricts 15 days, carry out secondary turning moisturizing.
3, fermentation period
Within about about 30 days, whole digest process completes, straw overstrike or pitchy;Windrow has the delicate fragrance of biofermentation
Earth taste, ammonia taste;Be covered with a large amount of white hypha in heap, texture is evacuated.
After fermentation ends, when can plough under field as required or continue composting to Rice Cropping in the coming year, plough under field as life
Thing bacterial manure uses, and is administered in farmland.Soil microbial activity can be promoted, improve soil organic matter content, within the specific limits
Maintain the dynamic equilibrium of soil with organic matter, improve the physical arrangement of soil, fixing and holding soil nitrogen nutriment, increase indissoluble
Property nutrient availability, recover and maintenance of soil fertility, solve to decline due to farming causes farmland quality year after year, soil is organic
The problems such as matter is not enough, and soil water retention, fertilizer conservation, venting capability reduce, soil aggregate minimizing, are greatly facilitated agricultural production.
After fermentation, the component analysis of the straw that becomes thoroughly decomposed sees table 1.
Table 1
Comparative example 1
The most same as in Example 1, difference is:
1, microbial bacterial agent, by weight
The composite bacteria agent capable prepared requires: acid protease >=2000u/g, neutral protease >=10000u/g, cellulose
Enzyme >=10000u/g, insufficient section adds corresponding enzyme preparation.
2, the microbial inoculum implanted in volcanic rock biological preparation is:
After fermenting dry straw as described in Example 1, the component analysis of the straw that becomes thoroughly decomposed sees table 2.
Table 2
Embodiment 1 contrasts (weight-loss ratio method) with comparative example 1 straw decomposing degree, and its result sees table 3.
Table 3
Above-mentioned test is that at the end of five months, the beginning of June Changchun northeastward is carried out.Because the Northeast Oryza sativa L. is only planted a season, typically
The annual October can gather in, and this time the time of test is similar for season to Jilin part Oryza sativa L. ripe and harvested in sky temperature, humidity,
Result of the test is made to have more persuasion.
Above the present invention is described in detail, its object is to allow the personage being familiar with this art will appreciate that this
Invention content and be carried out, can not limit the scope of the invention with this, and the invention is not restricted to above-mentioned enforcement
Example, all equivalence changes made according to the spirit of the present invention or modification, all should contain within protection scope of the present invention.
Claims (8)
1. a low temperature effective microorganism preparation product, including microbial bacterial agent, it is characterised in that: described microbial bacterial agent
It is CCTCC NoM208058 including the bacillus subtilis KX-1 that preserving number is CCTCC NoM208057, actinomycetes, preserving number
Bacillus subtilis KX-2, preserving number be the bacillus subtilis KX-4 of CCTCC NoM208060.
Low temperature effective microorganism preparation product the most according to claim 1, it is characterised in that: described bacillus subtilis
Bacterium KX-1, described actinomycetes, described bacillus subtilis KX-2, the consumption mass ratio of described bacillus subtilis KX-4
For 1:0.3 ~ 0.75:0.4 ~ 0.85:0.5 ~ 0.85.
Low temperature effective microorganism preparation product the most according to claim 1, it is characterised in that: described microbial bacterial agent is also
Including other beneficial microbes, other described beneficial microbes are selected from lactic acid bacteria, photosynthetic bacteria, yeast, lichens spore
One or more in bacillus.
Low temperature effective microorganism preparation product the most according to claim 3, it is characterised in that: described bacillus subtilis
KX-1 and other described beneficial microorganism consumption mass ratioes are 1:0.1 ~ 0.8.
Low temperature effective microorganism preparation product the most according to claim 1, it is characterised in that: described microbial bacterial agent is also
Including the enzyme preparation selectively added so that the acid protease >=2000u/g of described microbial bacterial agent, neutral protease >=
10000u/g, cellulase >=10000u/g.
Low temperature effective microorganism preparation product the most according to claim 1, it is characterised in that: the useful micro-life of described low temperature
Thing formulation products also includes that volcanic rock biological preparation, described volcanic rock biological preparation include volcanic rock particulate vector, are implanted in
The described microbial bacterial agent on volcanic rock particulate vector.
Low temperature effective microorganism preparation product the most according to claim 6, it is characterised in that: described volcanic rock biology system
The preparation method of agent comprises the steps:
Step (1), volcanic rock is milled to the volcanic rock granule of 20 ~ 40 mesh, after removing powder, obtains volcanic rock particulate vector;
Step (2), it is that 1:20 ~ 50 are configured to bacterium solution by described microbial bacterial agent and water according to consumption mass ratio, is subsequently adding
Strain is activated by nitrogen source and carbon source, when the bacillus cereus more than 80% in bacterium solution is in trophosome state, viable count > 5,000,000,000/
During mL, described volcanic rock particulate vector is soaked in described bacterium solution 24 ~ 72h, then described volcanic rock granule is carried
Body is pulled out and is drained, and obtains described volcanic rock biological preparation.
Low temperature effective microorganism preparation product the most according to claim 1, it is characterised in that: the useful micro-life of described low temperature
Thing formulation products also includes amino acid granular.
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