CN105037042B - It is a kind of using bio-feritlizer of the secondary aerobic fermentation of composite bacteria agent and preparation method thereof - Google Patents
It is a kind of using bio-feritlizer of the secondary aerobic fermentation of composite bacteria agent and preparation method thereof Download PDFInfo
- Publication number
- CN105037042B CN105037042B CN201510479084.6A CN201510479084A CN105037042B CN 105037042 B CN105037042 B CN 105037042B CN 201510479084 A CN201510479084 A CN 201510479084A CN 105037042 B CN105037042 B CN 105037042B
- Authority
- CN
- China
- Prior art keywords
- parts
- composite bacteria
- bacteria agent
- bio
- feritlizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 74
- 239000002131 composite material Substances 0.000 title claims abstract description 65
- 238000010564 aerobic fermentation Methods 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title claims description 13
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 64
- 210000003608 fece Anatomy 0.000 claims abstract description 27
- 240000008042 Zea mays Species 0.000 claims abstract description 23
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 23
- 239000010902 straw Substances 0.000 claims abstract description 22
- 150000001413 amino acids Chemical class 0.000 claims abstract description 21
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims abstract description 19
- 235000009973 maize Nutrition 0.000 claims abstract description 19
- 241000607269 Vibrio proteolyticus Species 0.000 claims abstract description 18
- 229930006000 Sucrose Natural products 0.000 claims abstract description 15
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 15
- 244000005700 microbiome Species 0.000 claims abstract description 15
- 239000000843 powder Substances 0.000 claims abstract description 14
- 235000009051 Ambrosia paniculata var. peruviana Nutrition 0.000 claims abstract description 13
- 235000003097 Artemisia absinthium Nutrition 0.000 claims abstract description 13
- 240000001851 Artemisia dracunculus Species 0.000 claims abstract description 13
- 235000017731 Artemisia dracunculus ssp. dracunculus Nutrition 0.000 claims abstract description 13
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims abstract description 13
- 239000001138 artemisia absinthium Substances 0.000 claims abstract description 13
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000005720 sucrose Substances 0.000 claims abstract description 13
- 241000589614 Pseudomonas stutzeri Species 0.000 claims abstract description 10
- 241000499912 Trichoderma reesei Species 0.000 claims abstract description 10
- 239000001913 cellulose Substances 0.000 claims abstract description 10
- 229920002678 cellulose Polymers 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 241000228347 Monascus <ascomycete fungus> Species 0.000 claims abstract description 9
- 239000003513 alkali Substances 0.000 claims abstract description 9
- 229910052586 apatite Inorganic materials 0.000 claims abstract description 8
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 claims abstract description 8
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 claims abstract description 5
- 229940024606 amino acid Drugs 0.000 claims description 19
- 238000000855 fermentation Methods 0.000 claims description 17
- 230000004151 fermentation Effects 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 13
- 239000010871 livestock manure Substances 0.000 claims description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
- 241000287828 Gallus gallus Species 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 5
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 claims description 4
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 4
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 4
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 4
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 4
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 229960003067 cystine Drugs 0.000 claims description 4
- 229960000310 isoleucine Drugs 0.000 claims description 4
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims description 4
- 229930182817 methionine Natural products 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 239000004474 valine Substances 0.000 claims description 4
- 230000032683 aging Effects 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims 5
- 239000007864 aqueous solution Substances 0.000 claims 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 abstract description 3
- 239000002689 soil Substances 0.000 description 19
- 239000003337 fertilizer Substances 0.000 description 13
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 5
- 239000005416 organic matter Substances 0.000 description 5
- 229910052700 potassium Inorganic materials 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 244000000626 Daucus carota Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- 210000000481 breast Anatomy 0.000 description 4
- 235000005473 carotenes Nutrition 0.000 description 4
- 239000004310 lactic acid Substances 0.000 description 4
- 235000014655 lactic acid Nutrition 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- 244000144977 poultry Species 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 235000002767 Daucus carota Nutrition 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- -1 carotene carotene Chemical class 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 244000113306 Monascus purpureus Species 0.000 description 1
- 235000002322 Monascus purpureus Nutrition 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 241000385540 bacterium 10 Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002515 guano Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000014786 phosphorus Nutrition 0.000 description 1
- 235000010958 polyglycerol polyricinoleate Nutrition 0.000 description 1
- 235000007686 potassium Nutrition 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Landscapes
- Fertilizers (AREA)
Abstract
The present invention provides a kind of bio-feritlizer using the secondary aerobic fermentation of composite bacteria agent, and the raw material of the bio-feritlizer includes the component of following weight proportion：300 330 parts of maize straw, 280 340 parts of cicken feces dried, 13 16 parts of amino acid solution, 40 48 parts of aqueous sucrose solution, 25 30 parts of the fluffy powder of alkali, 9 11 parts of wormwood, 57 parts of apatite, 3.8 4.4 parts of composite bacteria agent A, 3.2 3.6 parts of composite bacteria agent B.Composite bacteria agent A includes following microorganism：Pseudomonas stutzeri, bacillus stearothermophilus, vibrio proteolyticus.Composite bacteria agent B includes following microorganism：Trichoderma reesei, De Shi lactobacillus lactis, vibrio proteolyticus, monascus.The bio-feritlizer prepared using the present invention, amino acid content are 22 24%, and high molecular weight protein content is 19 23%, and content of cellulose reduces by 55 60%.
Description
Technical field
The invention belongs to Crop fertilizer field, more particularly to a kind of bio-fertilizers using the secondary aerobic fermentation of composite bacteria agent
Material and preparation method thereof.
Background technology
With the continuous development of economy and society and the foundation of intensive pasture, the yield of chicken manure is growing day by day, according to system
Meter, Chinese tens tons of feces of livestock and poultry yield in 2013, considerably beyond the total amount of current year industrial solid castoff, feces of livestock and poultry
Become the main source of city suburbs and rural environment, not only endangered ecological environment and human health, also affect livestock and poultry production
Industry sustainable development.
For resource view, chicken manure contains a certain amount of N P and K and various middle trace elements, warp based on organic matter
Processing is crossed, good agricultural fertilizer can be become.
Rural area maize straw yield is huge simultaneously, and a large amount of maize straw is incinerated and abandons, and not only causes environment dirty
Dye, and the soil organism is wasted serious.The elements such as the nitrogen, phosphorus, potassium, magnesium, calcium, the sulphur that contain in maize straw are crops lifes
Main nutrient elements necessary to length, therefore, maize straw are also abundant manure resources.The content of organic matter is averaged in maize straw
It is 15% or so, also containing quite a certain amount of ammonium bicarbonate of nitrogen, phosphorus, potassium, calcium superphosphate and potassium sulfate etc..So maize straw
Play the role of positive for improvement soil, summing up mainly has following three points：1st, soil nutrient content 2, energy can be improved
Enough improve soil property 3, farmland ecological environment can be optimized.
Today's society actively develops bio-feritlizer and organic fertilizer, mostly single strain or composite bacteria：A part is
Microorganism and the compound of chemical fertilizer；Only a small amount of is the compound of the organic matter of maize straw class, composite bacteria agent and feces of livestock and poultry
Body.And using chicken manure and corn stalk powder as fermentation raw material, composite bacteria agent is added, inquires into optimal conditions of fermentation, optimize technique reaches
To solving maize straw and chicken manure is innoxious and recycling purpose.
The development trend of international bio fertilizer is made a general survey of, is broadly divided into the following aspects：
1. being developed in terms of multi-functional from single function, include screening, combination and the use of joint flora, fertilizer function is to fertilizer
Effect unification etc..
2. developed from Inoculant to composite biological fertilizer.Not only it can increase beneficial rhizosphere flora, but also can to increase soil activation organic
Matter and nutrient make fertilizer and provide base for efficient bacterium proliferation and activity.
3. improve application effect.
It is 4. reasonable compound with nutrient or organic matter.
5. with PGPR（Plant roots circle growth-promoting bacterium）Use in conjunction.
The chicken manure of the prior art and the bio-feritlizer of maize straw fermenting and producing have following defect：（1）Amino acid content
Low, high molecular weight protein and content of cellulose are high, and plant is not easy to absorb and utilize；（2）Use the fermenting organism fertilizer of the prior art
It is poor to improve crop yield effect for material；（3）The fermenting organism fertilizer of the prior art improves growing area soil effect unobvious.
Invention content
The present invention provides a kind of bio-feritlizer using the secondary aerobic fermentation of composite bacteria agent and its preparation for above deficiency
Method realizes following goal of the invention：
1st, the amino acid content of bio-feritlizer is improved, reduces high molecular weight protein content and content of cellulose；Contain amino acid
It measures as 22-24%, high molecular weight protein content is 19-23%, and content of cellulose reduces 55-60%；
2nd, using bio-feritlizer of the present invention, crop-planting yield is improved；
3rd, using bio-feritlizer of the present invention, the quality of long-term cropping is improved；
4th, using bio-feritlizer of the present invention, hence it is evident that improve growing area soil, improve water capacity, porosity, nutrition member
Cellulose content.
In order to solve the above technical problems, using following technical scheme：
A kind of bio-feritlizer using the secondary aerobic fermentation of composite bacteria agent, the raw material of the bio-feritlizer is including following heavy
Measure the component of proportioning：300-330 parts of maize straw, 280-340 parts of cicken feces dried, 13-16 parts of amino acid solution, sucrose water
40-48 parts of solution, alkali are powder 25-30 parts fluffy, 9-11 parts of wormwood, 5-7 parts of apatite, 3.8-4.4 parts of composite bacteria agent A, compound
3.2-3.6 parts of microbial inoculum B.
It is that above-mentioned technical proposal is further improved below：
The composite bacteria agent A includes the microorganism of following content：Pseudomonas stutzeri 20 × 109CFU/g, thermophilic fat
Fat bacillus 10 × 109CFU/g, vibrio proteolyticus 0.5 × 109 CFU/g。
The composite bacteria agent B includes the microorganism of following content：Trichoderma reesei 13 × 109CFU/g, De Shi lactic acid breast
Bacillus 15 × 109CFU/g, vibrio proteolyticus 1.5 × 109CFU/g, monascus 15 × 109 CFU/g。
The raw material of the bio-feritlizer includes the component of following weight proportion：310 parts of maize straw, cicken feces dried 300
Part, 14.5 parts of amino acid solution, 44 parts of aqueous sucrose solution, 27 parts of the fluffy powder of alkali, 10 parts of wormwood, 6 parts of apatite, compound bacteria
4.1 parts of agent A, 3.4 parts of composite bacteria agent B.
The preparation method prepare composite bacteria agent A, prepare composite bacteria agent B, decomposed base-material preparation, for the first time fermentation,
Second of fermentation step.
The preparation process of the decomposed base-material is included disintegrating slag of the corn straw smashing for 0.2-1cm；Chicken manure is ground, carefully
It spends for 40 mesh.
The first time fermentation step：Composite bacteria agent A is sprinkled into decomposed base-material, is uniformly sprinkled into, 80rpm stirring 20min,
The decomposed heap of about 1.5 meters of height, fermentation are piled, every five days turning and throwing onces are fermented 20 days.
Second of fermentation step：Composite bacteria agent B is sprinkled into decomposed base-material, is uniformly sprinkled into, 100rpm stirring 10min,
The decomposed heap of about 1 meter of height is piled, carries out room temperature ageing fermentation, every 1 day turning and throwing once is fermented 10 days.
The bio-feritlizer prepared using the present invention, amino acid content 22-24%, high molecular weight protein content is 19-23%, fine
Tieing up cellulose content reduces 55-60%.
Compared with prior art the invention has the advantages that：
1st, the bio-feritlizer prepared using the present invention, amino acid content 22-24%, high molecular weight protein content are 19-23%,
Content of cellulose reduces 55-60%；
2nd, bio-feritlizer nutrient of the present invention is complete, is easily absorbed by plants, hence it is evident that improves crop-planting yield；Using this
Invention bio-feritlizer plants carrot, and yield is 6500-6800kg/ mus；
3rd, the quality of long-term cropping is significantly improved using bio-feritlizer of the present invention；The Hu planted using bio-feritlizer of the present invention
Radish：Protein content is 0.68-0.72 g/100g, and calcium content is 42-45 mg/100g, carotene carotene content 3.82-
3.86mg/100g；
4th, bio-feritlizer of the present invention improves significantly to soil, after bio-feritlizer of the present invention 1 year, to plantation
Area's soil has clear improvement, and water capacity improves 9-12%, and soil porosity improves 12.8-15.4%, and soil total N content carries
High 22.1-24.6%, available potassium in soils content improve 43.2-45.6%.
Specific embodiment
The present invention is described further with reference to embodiments.
In the present invention, Pseudomonas stutzeri（Pseudomonas stutzeri）Preserving number be ACCC 01977, thermophilic fat
Fat bacillus（Bacillus stearothermophilus）Preserving number be CGMCC 1.1923, vibrio proteolyticus
（Vibrio proteolyticus）Preserving number is MCCC1H00046, trichoderma reesei（Trichoderma reesei）Preserving number
For ACCC30911, De Shi lactobacillus lactis（Lactobacillus delbrueckii）Preserving number is ACCC10183, monascus
（Monascus anka）Preserving number is ACCC30341, and above-mentioned strain is all from commercially available.
A kind of bio-feritlizer using the secondary aerobic fermentation of composite bacteria agent of embodiment 1
The raw material of the bio-feritlizer includes the component of following weight proportion：
300 parts of maize straw, 280 parts of cicken feces dried, 16 parts of amino acid solution, 40 parts of aqueous sucrose solution, the fluffy powder of alkali
25 parts, 9 parts of wormwood, 5 parts of apatite, 4.4 parts of composite bacteria agent A, 3.2 parts of composite bacteria agent B；
The cicken feces dried：Water content is 13%；
The amino acid solution：Containing leucine 3%, isoleucine 7%, valine 6%, cystine 5%, methionine
9%；
The aqueous sucrose solution：A concentration of 60%；
The wormwood：For 80 mesh powder of fineness, water content 7%；
The composite bacteria agent A includes the microorganism of following content：Pseudomonas stutzeri 20 × 109CFU/g, thermophilic fat
Fat bacillus 10 × 109CFU/g, vibrio proteolyticus 0.5 × 109CFU/g；
The composite bacteria agent B includes the microorganism of following content：Trichoderma reesei 13 × 109CFU/g, De Shi lactic acid breast
Bacillus 15 × 109CFU/g, vibrio proteolyticus 1.5 × 109CFU/g, monascus 15 × 109 CFU/g。
Above-mentioned percentage is mass percent.
A kind of bio-feritlizer using the secondary aerobic fermentation of composite bacteria agent of embodiment 2
The raw material of the bio-feritlizer includes the component of following weight proportion：
310 parts of maize straw, 300 parts of cicken feces dried, 14.5 parts of amino acid solution, 44 parts of aqueous sucrose solution, alkali are fluffy
27 parts of powder, 10 parts of wormwood, 6 parts of apatite, 4.1 parts of composite bacteria agent A, 3.4 parts of composite bacteria agent B；
The cicken feces dried：Water content is 13%；
The amino acid solution：Containing leucine 3%, isoleucine 7%, valine 6%, cystine 5%, methionine
9%；
The aqueous sucrose solution：A concentration of 60%；
The wormwood：For 80 mesh powder of fineness, water content 7%；
The composite bacteria agent A includes the microorganism of following content：Pseudomonas stutzeri 20 × 109CFU/g, thermophilic fat
Fat bacillus 10 × 109CFU/g, vibrio proteolyticus 0.5 × 109CFU/g；
The composite bacteria agent B includes the microorganism of following content：Trichoderma reesei 13 × 109CFU/g, De Shi lactic acid breast
Bacillus 15 × 109CFU/g, vibrio proteolyticus 1.5 × 109CFU/g, monascus 15 × 109 CFU/g。
Above-mentioned percentage is mass percent.
A kind of bio-feritlizer using the secondary aerobic fermentation of composite bacteria agent of embodiment 3
The raw material of the bio-feritlizer includes the component of following weight proportion：
330 parts of maize straw, 340 parts of cicken feces dried, 13 parts of amino acid solution, 48 parts of aqueous sucrose solution, the fluffy powder of alkali
30 parts, 11 parts of wormwood, 7 parts of apatite, 3.8 parts of composite bacteria agent A, 3.6 parts of composite bacteria agent B；
The cicken feces dried：Water content is 13%；
The amino acid solution：Containing leucine 3%, isoleucine 7%, valine 6%, cystine 5%, methionine
9%；
The aqueous sucrose solution：A concentration of 60%；
The wormwood：For 80 mesh powder of fineness, water content 7%；
The composite bacteria agent A includes the microorganism of following content：Pseudomonas stutzeri 20 × 109CFU/g, thermophilic fat
Fat bacillus 10 × 109CFU/g, vibrio proteolyticus 0.5 × 109CFU/g；
The composite bacteria agent B includes the microorganism of following content：Trichoderma reesei 13 × 109CFU/g, De Shi lactic acid breast
Bacillus 15 × 109CFU/g, vibrio proteolyticus 1.5 × 109CFU/g, monascus 15 × 109 CFU/g。
Above-mentioned percentage is mass percent.
A kind of preparation method of the bio-feritlizer using the secondary aerobic fermentation of composite bacteria agent of embodiment 4
Step 1 prepares composite bacteria agent A and composite bacteria agent B
A. composite bacteria agent A is prepared：
Respectively by Pseudomonas stutzeri, bacillus stearothermophilus and vibrio proteolyticus；It carries out actication of culture, spread cultivation, train
Nutrient solution obtains bacteria suspension by centrifugation, separation bacteria residue；Bacteria suspension is mixed and is sprayed on carrier, and the composite bacteria agent of pulvis is made
A；
The composite bacteria agent A water content is 28%；
The carrier is starch and the mixture of white sugar, and mixed weight ratio is 3：0.5；
Content of microorganisms is in the composite bacteria agent A：Pseudomonas stutzeri 20 × 109CFU/g, stearothermophilus gemma bar
Bacterium 10 × 109CFU/g, vibrio proteolyticus 0.5 × 109CFU/g；
B. composite bacteria agent B is prepared：
Respectively by trichoderma reesei, De Shi lactobacillus lactis, vibrio proteolyticus and monascus；It carries out actication of culture, spread cultivation,
Culture solution obtains bacteria suspension by centrifugation, separation bacteria residue；Bacteria suspension is mixed and is sprayed on carrier, and the compound bacteria of pulvis is made
Agent B；
The composite bacteria agent B water content is 34%；
The carrier is starch；
Content of microorganisms is in the composite bacteria agent B：Trichoderma reesei 13 × 109CFU/g, De Shi lactobacillus lactis 15
×109CFU/g, vibrio proteolyticus 1.5 × 109CFU/g, monascus 15 × 109 CFU/g。
The preparation of step 2, decomposed base-material
A. by disintegrating slag of the corn straw smashing for 0.2-1cm；Chicken manure is ground, fineness is 40 mesh；
B. each raw material is weighed by formula, it is spare；
C. by maize straw, cicken feces dried, the fluffy powder of alkali, wormwood, apatites mixed, 60rpm is stirred evenly, in whipping process
Amino acid solution and aqueous sucrose solution are sprayed into, obtains decomposed base-material.
Step 3 is fermented for the first time
To step（2）Composite bacteria agent A is sprinkled into ready decomposed base-material, is uniformly sprinkled into, 80rpm stirring 20min are piled
The decomposed heap of about 1.5 meters of height, fermentation, every five days turning and throwing onces are fermented 20 days.
Step 4 is fermented for second
For the first time after fermentation, decomposed heap is spread out, is dried in the air to room temperature；Composite bacteria agent B is sprinkled into, is uniformly sprinkled into, 100rpm
10min is stirred, piles the decomposed heap of about 1 meter of height, carries out room temperature ageing fermentation, every 1 day turning and throwing once is fermented 10 days, obtained
Refined biometric fertilizer.
As a result it detects：
（1）Bio-feritlizer of the present invention is detected, the results are shown in Table 1；
The bio-feritlizer testing result of 1 embodiment 1-3 of table：
By table 1 as it can be seen that preparing bio-feritlizer using the present invention, hence it is evident that improve the amino acid content of fertilizer, reduce macromolecular
Protein content and content of cellulose, amino acid content 22-24%, high molecular weight protein content are 19-23%, and content of cellulose reduces
55-60%。
（2）The bio-feritlizer of embodiment 1-3 is used for the plantation of carrot as base manure, with common brid guano and maize straw
Organic fertilizer is control group, and sowing amount is 200kg/ mus；In addition to the base manure used, other implantation methods all sames plant field
Edaphic condition it is also identical, planting experiment result is detected after plantation；The results are shown in Table 1：
The bio-feritlizer of 2 embodiment 1-3 of table is used for the effect of growth of carrot
As can be seen from Table 2, planting carrot using bio-feritlizer of the present invention, yield is 6500-6800kg/ mus, the Hu Luo of plantation
Foretell：Protein content is 0.68-0.72 g/100g, and calcium content is 42-45 mg/100g, carotene carotene content 3.82-
3.86mg/100g。
（2）After the bio-feritlizer 1 year of embodiment 1-3, plantation field soil is detected, the results are shown in Table 2：
Soil root system of the table 3 using the bio-feritlizer of embodiment 1-3 after 1 year
By table 3 as it can be seen that after 1 year, being obviously improved using bio-feritlizer of the present invention to growing area soil, soil water retention
Amount improves 9-12%, and soil porosity improves 12.8-15.4%, and soil total N content improves 22.1-24.6%, and available potassium in soils contains
Amount improves 43.2-45.6%.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
It, for those skilled in the art, still can be with although the present invention is described in detail with reference to the foregoing embodiments
It modifies to the technical solution recorded in foregoing embodiments or equivalent replacement is carried out to which part technical characteristic.It is all
Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in the guarantor of the present invention
Within the scope of shield.
Claims (2)
1. a kind of bio-feritlizer using the secondary aerobic fermentation of composite bacteria agent, it is characterised in that：The raw material of the bio-feritlizer
Include the component of following weight proportion：300-330 parts of maize straw, 280-340 parts of cicken feces dried, amino acid solution 13-16
Part, 40-48 parts of aqueous sucrose solution, alkali are powder 25-30 parts fluffy, 9-11 parts of wormwood, 5-7 parts of apatite, composite bacteria agent A 3.8-
4.4 parts, 3.2-3.6 parts of composite bacteria agent B；
The composite bacteria agent A includes the microorganism of following content：Pseudomonas stutzeri 20 × 109Cfu/g, stearothermophilus bud
Spore bacillus 10 × 109Cfu/g, vibrio proteolyticus 0.5 × 109cfu/g；
The composite bacteria agent A water content is 28%；
The carrier of the composite bacteria agent A is starch and the mixture of white sugar, and mixed weight ratio is 3：0.5；
The composite bacteria agent B includes the microorganism of following content：Trichoderma reesei 13 × 109Cfu/g, De Shi lactobacillus lactis
15×109Cfu/g, vibrio proteolyticus 1.5 × 109Cfu/g, monascus 15 × 109cfu/g；
The composite bacteria agent B water content is 34%；
The carrier of the composite bacteria agent B is starch；
The cicken feces dried：Water content is 13%；
The amino acid solution：Containing leucine 3%, isoleucine 7%, valine 6%, cystine 5%, methionine
9% ；
The aqueous sucrose solution：A concentration of 60%；
The wormwood：For 80 mesh powder of fineness, water content 7%；
The bio-feritlizer of preparation, small molecular protein content are 22-24%, and high molecular weight protein content is 19-23%, and content of cellulose drops
Low 55-60%；
The preparation method prepares composite bacteria agent A, prepares composite bacteria agent B, for the first time preparation of decomposed base-material, fermentation, second
Secondary fermentation step；
The preparation process of the decomposed base-material is included disintegrating slag of the corn straw smashing for 0.2-1cm；Chicken manure is ground, fineness is
40 mesh；
The first time fermentation step：Composite bacteria agent A is sprinkled into decomposed base-material, is uniformly sprinkled into, 80rpm stirring 20min are piled
The decomposed heap of about 1.5 meters of height, fermentation, every five days turning and throwing onces are fermented 20 days；
Second of fermentation step：Composite bacteria agent B is sprinkled into decomposed base-material, is uniformly sprinkled into, 100rpm stirring 10min are piled
The decomposed heap of about 1 meter of height, carries out room temperature ageing fermentation, and every 1 day turning and throwing once is fermented 10 days.
2. a kind of bio-feritlizer using the secondary aerobic fermentation of composite bacteria agent according to claim 1, it is characterised in that：Institute
The raw material for the bio-feritlizer stated includes the component of following weight proportion：310 parts of maize straw, 300 parts of cicken feces dried, amino acid
14.5 parts of aqueous solution, 44 parts of aqueous sucrose solution, 27 parts of the fluffy powder of alkali, 10 parts of wormwood, 6 parts of apatite, composite bacteria agent A 4.1
Part, 3.4 parts of composite bacteria agent B.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510479084.6A CN105037042B (en) | 2015-08-03 | 2015-08-03 | It is a kind of using bio-feritlizer of the secondary aerobic fermentation of composite bacteria agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510479084.6A CN105037042B (en) | 2015-08-03 | 2015-08-03 | It is a kind of using bio-feritlizer of the secondary aerobic fermentation of composite bacteria agent and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105037042A CN105037042A (en) | 2015-11-11 |
| CN105037042B true CN105037042B (en) | 2018-06-22 |
Family
ID=54444096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510479084.6A Expired - Fee Related CN105037042B (en) | 2015-08-03 | 2015-08-03 | It is a kind of using bio-feritlizer of the secondary aerobic fermentation of composite bacteria agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105037042B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107473867B (en) * | 2017-10-09 | 2020-12-22 | 山东沃科奇实业有限公司 | Biological bacterial fertilizer prepared from livestock and poultry waste and preparation method thereof |
| CN107857689A (en) * | 2017-12-28 | 2018-03-30 | 青岛嘉瑞生物技术有限公司 | The technique that a kind of bioanalysis prepares bio-organic fertilizer using chicken manure |
| CN109020758A (en) * | 2018-10-11 | 2018-12-18 | 湖北文理学院 | A kind of base manure and preparation method thereof suitable for Xiangyang yam planting |
| CN113620752A (en) * | 2021-09-06 | 2021-11-09 | 广东汇先丰农牧有限公司 | Chicken manure organic fertilizer rich in microbial strains and preparation method thereof |
| CN113956088A (en) * | 2021-11-29 | 2022-01-21 | 江苏农乐生物科技有限公司 | Fertilizer containing xylo-oligosaccharide and application of fertilizer to promoting watermelon growth |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101172896A (en) * | 2007-10-18 | 2008-05-07 | 天津南开大学蓖麻工程科技有限公司 | Biological organic fertilizer and method for producing the same |
| CN104261911A (en) * | 2014-09-22 | 2015-01-07 | 烟台中正新技术有限公司 | Method for preparing organic fertilizer by using plant residues as raw material |
| CN104609912A (en) * | 2014-12-19 | 2015-05-13 | 角志海 | A composite biological bacterial fertilizer based on a novel composite biological live bacterial preparation and a preparing method thereof |
-
2015
- 2015-08-03 CN CN201510479084.6A patent/CN105037042B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101172896A (en) * | 2007-10-18 | 2008-05-07 | 天津南开大学蓖麻工程科技有限公司 | Biological organic fertilizer and method for producing the same |
| CN104261911A (en) * | 2014-09-22 | 2015-01-07 | 烟台中正新技术有限公司 | Method for preparing organic fertilizer by using plant residues as raw material |
| CN104609912A (en) * | 2014-12-19 | 2015-05-13 | 角志海 | A composite biological bacterial fertilizer based on a novel composite biological live bacterial preparation and a preparing method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105037042A (en) | 2015-11-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103342615B (en) | A kind of phosphorus decomposing, potassium decomposing biological organic fertilizer and preparation method thereof | |
| CN102212494B (en) | Organic matter decomposing inoculant, and preparation method and application thereof | |
| CN101898915B (en) | Microbial agent suitable for crops in seedling stage and preparation method thereof | |
| CN106434430A (en) | Compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof | |
| CN105037042B (en) | It is a kind of using bio-feritlizer of the secondary aerobic fermentation of composite bacteria agent and preparation method thereof | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN103964946A (en) | Special compound microbial fertilizer for paddy rice and preparation method thereof | |
| CN101092314A (en) | Biologic organic, inorganic composite fertilizer | |
| CN104498040B (en) | Application of the bacillus amyloliquefaciens as soil conditioner | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN107879852A (en) | A kind of edaphon modifying agent and preparation method thereof | |
| CN105565989A (en) | Bioorganic-inorganic composite fertilizer containing chitin and peat, and application thereof | |
| CN107056521A (en) | A kind of microbial manure and its manufacture method that getting fat is improved for salt affected soil | |
| CN112501072B (en) | Crop rhizosphere soil conditioner and preparation method and application thereof | |
| CN103435395A (en) | Drip-irrigation type salt stress-alleviating and disease-preventing biological bacterial fertilizer and preparation method thereof | |
| CN106190929B (en) | A kind of new strain of Bacillus licheniformis and its application | |
| CN109679884A (en) | One plant of efficient Promoting bacteria of corn that can be reduced nitrogen phosphorus fertilizer application and its application | |
| CN111440034A (en) | Soil conditioner and preparation method and application thereof | |
| CN106396937A (en) | Complex microorganism rhzomorph modifier for saline-alkali soil and prepration method thereof | |
| CN102417412A (en) | Humic acid microbial fertilizer and preparation method thereof | |
| CN105061003A (en) | Microbial organic fertilizer and preparation method thereof | |
| CN110066194A (en) | Novel more bacterium composite microbe fertilizers of one kind and its preparation method and application | |
| CN106431609B (en) | Ginseng Chinese yam peanut specific complex continuous cropping resisting biological microbial inoculum and preparation method thereof | |
| CN106635905A (en) | Ecological restoration preparation for degraded soil obtained after cultivation of greenhouse vegetables, and preparation method of ecological restoration preparation | |
| CN107857681A (en) | A kind of special fertilizer for cole and preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180622 |