CN112501072B - Crop rhizosphere soil conditioner and preparation method and application thereof - Google Patents

Crop rhizosphere soil conditioner and preparation method and application thereof Download PDF

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CN112501072B
CN112501072B CN202011472044.6A CN202011472044A CN112501072B CN 112501072 B CN112501072 B CN 112501072B CN 202011472044 A CN202011472044 A CN 202011472044A CN 112501072 B CN112501072 B CN 112501072B
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soil conditioner
rhizosphere soil
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毛欣宇
姜小三
于怀志
徐洲
翟森茂
陈丽娜
邵孝侯
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Jiangsu Enste Ecological Technology Co ltd
Taizhou Research Institute Of Nanjing Agricultural University
Hohai University HHU
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Taizhou Research Institute Of Nanjing Agricultural University
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Abstract

The invention provides a crop rhizosphere soil conditioner, a preparation method and application thereof, and belongs to the technical field of agriculture. The crop rhizosphere soil conditioner provided by the invention can improve the soil rhizosphere microenvironment, and can quickly and effectively solve the problem of poor root system growth caused by poor soil environment. Promote the growth of crop root system, raise yield and quality, improve soil and health soil, raise crop resistance and reduce diseases.

Description

Crop rhizosphere soil conditioner and preparation method and application thereof
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a crop rhizosphere soil conditioner, and a preparation method and application thereof.
Background
The pig farm water-soaking manure process culture or the dairy farm automatic manure scraping and back flushing process culture in the large-scale culture industry is used as a main culture mode at present, and the treatment and utilization of a large amount of manure sewage (the solid content is only 15%) are still the primary problems of the existing manure treatment. Currently, in terms of handling and utilization: the fertilizer is mainly stored in a storage tank for utilization, or the manure and sewage are discharged after reaching standards, the storage period is too long (three months to six months), enough land is needed for constructing an oxidation pond, the pollution to the surrounding environment is easy to cause in the storage process, the treatment cost is high, the fertilizer nutrient is reduced, and the fertilizer efficiency is obviously deteriorated; if the plant rhizosphere soil conditioner is used as a raw material of the plant rhizosphere soil conditioner, the plant rhizosphere soil conditioner is prepared through rapid fermentation treatment, so that the application effect is improved, and the plant rhizosphere soil conditioner is an effective way for solving the problem of high-efficiency utilization of livestock manure sewage.
The root system is the root of the growth of crops, is an essential organ for the crops to absorb moisture and nutrient from soil, and plays an important role in fixing plants, absorbing moisture, inorganic nutrition and a small amount of organic nutrition required by the growth and development of the crops and synthesizing a growth regulating substance. The plant root system is good, and moisture and nutrition can be better absorbed, so that the requirements of various growth and development of crops are met; the support function can be perfectly achieved, and the lodging caused by too many crop results and the like is avoided; it can also synthesize various hormones to meet the demands of crops, and can store and supply more nutrients. Therefore, the quality of the root system of the crop directly affects the yield and quality of the crop.
Soil is an important influencing factor for plant root system development. In order to obtain the greatest benefit from the soil, the present planting is not suitable for the cultivation of the soil, the planting is carried out year by year, and the chemical fertilizer is applied to harden, acidify and salinize the soil, so that the rhizosphere environment of the soil is poor, and the growth of root systems is not facilitated. Especially, some bare root seedlings are planted in a fixed mode, the seedling recovery period is long, the survival is difficult, the lodging resistance of the rice is reduced, and the like.
At present, the effective adjustment of the soil rhizosphere microenvironment and the increase of the number of beneficial bacteria can be changed by increasing the number of organic fertilizer, humic acid and fulvic acid. However, it is difficult to achieve the desired effect with a single application.
Disclosure of Invention
In view of the above, the invention aims to provide a crop rhizosphere soil conditioner, a preparation method and application thereof, and the crop rhizosphere soil conditioner provided by the invention can improve the soil rhizosphere microenvironment and can rapidly and effectively solve the problem of poor root system growth caused by poor soil environment.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a crop rhizosphere soil conditioner, the quantity of paenibacillus polymyxa in the crop rhizosphere soil conditioner is 4-5 multiplied by 10 8 cfu/mL, the quantity of the bacillus licheniformis is 3 to 5 multiplied by 10 6 cfu/mL, the quantity of the bacillus laterosporus is 2-4 multiplied by 10 8 cfu/mL, the number of the Ming's sacculus mildew is 1-2 multiplied by 10 8 cfu/mL, the quantity of the rhizopus in the root is 2-3 multiplied by 10 8 cfu/mL, the quantity of the Mortierella gemmifera is 1.5-2 multiplied by 10 8 cfu/mL, the number of young sleeve-type Ming's sacculus mildew is 1.5-2 multiplied by 10 8 cfu/mL。
The invention also provides a preparation method of the crop rhizosphere soil conditioner, which comprises the following steps:
1) Inoculating Paenibacillus polymyxa, bacillus licheniformis and Bacillus laterosporus into propagation medium, and culturing until the total microorganism content in the culture solution is 2×10 9 When cfu/mL is more than or equal to the cfu/mL, mixing the culture solution with the EM bacterial solution, and then carrying out aerobic fermentation to obtain a composite bacterial solution, wherein the total content of microorganisms in the composite bacterial solution is 2.2 multiplied by 10 9 cfu/mL or more;
2) Mixing the composite bacterial liquid obtained in the step 1), soybean powder, brown sugar and fecal sewage, fermenting for 8-20 d at 10-35 ℃ to obtain fermentation liquor, and filtering the fermentation liquor to obtain filtrate;
3) Mixing the filtrate obtained in the step 2) with composite endophyte, and standing for 3-4 d at 20-25 ℃ to obtain a crop rhizosphere soil conditioner;
the composite endophyte root fungus comprises Ming's sacculus mould, root inner sacculus mould, mortierella gemini and young Leuconostoc.
Preferably, the propagation medium in the step 1) takes the waste water without slag as a solvent, and each liter of the propagation medium comprises 0.2 to 0.3g/L of molasses, 0.3 to 0.5g/L of corn meal, 0.05 to 0.1g/L of ammonium sulfate, 0.05 to 0.08g/L of sodium acetate, 0.01 to 0.02g/L of magnesium sulfate and 0.01 to 0.03g/L of potassium dihydrogen phosphate.
Preferably, the conditions of the cultivation and the aerobic fermentation in the step 1) respectively include: the temperature is 30-35 ℃, and the stirring is carried out for 1 time every 2-3 hours for 10-15 min each time.
Preferably, the volume ratio of the culture solution in the step 1) to the EM bacterial solution is 0.5-1.5:0.5-1.5;
the preparation method of the EM bacterial liquid comprises the following steps: and mixing the EM bacteria stock solution with deionized water according to the volume ratio of 1:99.
Preferably, the mass ratio of the compound bacterial liquid, the soybean powder, the brown sugar and the fecal sewage in the step 2) is 0.5-1.5:0.5-1.5:8-12; the fecal sewage is slag-free fecal sewage.
Preferably, the mass ratio of the filtrate in the step 3) to the composite endophyte is 10-12:1.
Preferably, the spore quantity ratio of the Ming's saccule mold, the rhizopus radiatus, the Fabricius mosaicus and the young sleeve near Ming's saccule mold is 0.5-1.5:0.5-1.5;
the total spore number of the composite endophyte is 70-90/g.
The invention also provides application of the crop rhizosphere soil conditioner in improving soil according to the environment.
The invention provides a crop rhizosphere soil conditioner, a preparation method and application thereof, wherein the crop rhizosphere soil conditioner comprises a main component and a main componentThe quantity of the paenibacillus polymyxa in the crop rhizosphere soil conditioner is 4-5 multiplied by 10 8 cfu/mL, the quantity of the bacillus licheniformis is 3 to 5 multiplied by 10 6 cfu/mL, the quantity of the bacillus laterosporus is 2-4 multiplied by 10 8 cfu/mL, the number of the Ming's sacculus mildew is 1-2 multiplied by 10 8 cfu/mL, the quantity of the rhizopus in the root is 2-3 multiplied by 10 8 cfu/mL, the quantity of the Mortierella gemmifera is 1.5-2 multiplied by 10 8 cfu/mL, the number of young sleeve-type Ming's sacculus mildew is 1.5-2 multiplied by 10 8 cfu/mLL. Can improve the rhizosphere microenvironment of the soil and quickly and effectively solve the problem of poor root growth caused by poor soil environment. Promote the growth of crop root system, raise yield and quality, improve soil and health soil, raise crop resistance and reduce diseases.
Detailed Description
The invention provides a crop rhizosphere soil conditioner, the quantity of paenibacillus polymyxa in the crop rhizosphere soil conditioner is 4-5 multiplied by 10 8 cfu/mL, the quantity of the bacillus licheniformis is 3 to 5 multiplied by 10 6 cfu/mL, the quantity of the bacillus laterosporus is 2-4 multiplied by 10 8 cfu/mL, the number of the Ming's sacculus mildew is 1-2 multiplied by 10 8 cfu/mL, the quantity of the rhizopus in the root is 2-3 multiplied by 10 8 cfu/mL, the quantity of the Mortierella gemmifera is 1.5-2 multiplied by 10 8 cfu/mL, the number of young sleeve-type Ming's sacculus mildew is 1.5-2 multiplied by 10 8 cfu/mL。
The invention also provides a preparation method of the crop rhizosphere soil conditioner, which comprises the following steps:
1) Inoculating Paenibacillus polymyxa, bacillus licheniformis and Bacillus laterosporus into propagation medium, and culturing until the total microorganism content in the culture solution is 2×10 9 When cfu/mL is more than or equal to the cfu/mL, mixing the culture solution with the EM bacterial solution, and then carrying out aerobic fermentation to obtain a composite bacterial solution, wherein the total content of microorganisms in the composite bacterial solution is 2.2 multiplied by 10 9 cfu/mL or more;
2) Mixing the composite bacterial liquid obtained in the step 1), soybean powder, brown sugar and fecal sewage, fermenting for 8-20 d at 10-35 ℃ to obtain fermentation liquor, and filtering the fermentation liquor to obtain filtrate;
3) Mixing the filtrate obtained in the step 2) with composite endophyte, and standing for 3-4 d at 20-25 ℃ to obtain a crop rhizosphere soil conditioner;
the composite endophyte root fungus comprises Ming's sacculus mould, root inner sacculus mould, mortierella gemini and young Leuconostoc.
The invention inoculates Paenibacillus polymyxa, bacillus licheniformis and Bacillus laterosporus in the propagation culture medium for culture, when the total content of microorganisms in the culture solution is 2 multiplied by 10 9 When cfu/mL is more than or equal to the cfu/mL, mixing the culture solution with the EM bacterial solution, and then carrying out aerobic fermentation to obtain a composite bacterial solution, wherein the total content of microorganisms in the composite bacterial solution is 2.2 multiplied by 10 9 cfu/mL or more.
In the invention, the inoculation amount of the paenibacillus polymyxa inoculated to the propagation medium is preferably 5-10%, and the paenibacillus polymyxa is preferably inoculated in the form of paenibacillus polymyxa bacterial liquid; the method for obtaining the Paenibacillus polymyxa bacterial liquid is not particularly limited, and a method for obtaining the bacterial liquid by conventional culture of Paenibacillus polymyxa by a person skilled in the art can be adopted. In the invention, the paenibacillus polymyxa enhances the buffer capacity of soil, improves the structure, and can enhance the stress resistance, pathogen infection resistance, disease resistance, insect resistance and the like of crops.
In the invention, the inoculation amount of the bacillus licheniformis in the propagation medium is preferably 5-10%, and the bacillus licheniformis is preferably inoculated in the form of bacillus licheniformis bacterial liquid; the method for obtaining the bacillus licheniformis bacterial liquid is not particularly limited, and a method for obtaining the bacterial liquid by conventional culturing of bacillus licheniformis by a person skilled in the art is adopted. In the invention, the bacillus licheniformis can strengthen the buffer capacity of soil, improve the structure, and can strengthen the stress resistance of crops, resist pathogenic bacteria infection, resist diseases and insects and the like.
In the invention, the inoculation amount of the bacillus laterosporus inoculated to the propagation medium is preferably 5-10%, and the bacillus laterosporus is preferably inoculated in the form of bacillus laterosporus bacterial liquid; the method for obtaining the bacillus laterosporus bacterial liquid is not particularly limited, and a method for obtaining the bacterial liquid by conventional culture of bacillus laterosporus by a person skilled in the art is adopted. In the invention, the bacillus laterosporus has the functions of promoting root growth, resisting pathogenic bacteria dip-dyeing and degrading heavy metals.
In the invention, the propagation medium preferably takes the waste water without slag as a solvent, and each liter of the propagation medium comprises 0.2 to 0.3g/L of molasses, 0.3 to 0.5g/L of corn meal, 0.05 to 0.1g/L of ammonium sulfate, 0.05 to 0.08g/L of sodium acetate, 0.01 to 0.02g/L of magnesium sulfate and 0.01 to 0.03g/L of potassium dihydrogen phosphate. The invention preferably collects the manure sewage of the cattle farm or pig farm on the same day, filters out solid parts to prepare slag-free manure sewage, and the slag-free manure sewage contains rich organic matters, nitrogen, phosphorus, potassium, calcium magnesium, sulfur, chlorine, copper, zinc, iron, manganese, boron and the like, thereby providing microorganism and crop nutrition. In the present invention, the conditions of the culture preferably include: the temperature is 30-35 ℃, and the stirring is carried out for 1 time every 2-3 hours for 10-15 min each time.
In the invention, the volume ratio of the culture solution to the EM bacterial solution is preferably 0.5-1.5:0.5-1.5, more preferably 1:1; the preparation method of the EM bacterial liquid preferably comprises the following steps: and mixing the EM bacteria stock solution with deionized water according to the volume ratio of 1:99. In the invention, the EM stock solution is purchased from Aijiawe environmental protection biotechnology (Nanjing) limited company (commercial product), and the promoted EM strain EM raw material is Japan-specific Jia-Zhuang invention authentic EM1 stock solution/1 KG x 12 bottle. In the present invention, the conditions of the aerobic fermentation preferably include: the temperature is 30-35 ℃, and the stirring is carried out for 1 time every 2-3 hours for 10-15 min each time.
In the invention, the pH value of the composite bacterial liquid is preferably 3.5-4.0, and the total microorganism content in the composite bacterial liquid is 1 multiplied by 10 9 cfu/mL or more.
The invention mixes the obtained composite bacterial liquid, soybean powder, brown sugar and fecal sewage, ferments for 8-20 d at 10-35 ℃ to obtain fermentation liquor, and filters the fermentation liquor to obtain filtrate.
In the invention, soybeans purchased in the market are dried at 60-80 ℃, crushed and sieved by a sieve of 80-100 meshes to prepare soybean powder, and the soybean powder is fermented to contain rich proteins, amino acids, vitamins and various trace elements to mainly provide nitrogen nutrition and can be used as nutrient substances for microorganisms and crops.
In the invention, the brown sugar is preferably purchased from a commercial product, and contains 96.6g of carbohydrate per 100g, and also contains elements such as potassium, phosphorus, calcium, magnesium, iron, manganese, zinc, copper and the like as microorganism nutrient substances, so as to adjust the carbon-nitrogen ratio of the fermentation mixture.
In the invention, the mass ratio of the composite bacterial liquid to the soybean meal to the brown sugar to the fecal sewage is preferably 0.5-1.5:0.5-1.5:8-12, and more preferably 1:1:1:10. In the invention, the fecal sewage is preferably slagless fecal sewage, and the source is the same as the above, and will not be described again. In the invention, the non-slag fecal sewage can accelerate the propagation speed of microorganisms.
In the invention, the composite bacterial liquid, soybean powder, brown sugar and fecal sewage are mixed and fermented for 8-20 d at the temperature of 10-35 ℃ to obtain fermentation liquor. When the temperature is 10-15 ℃, the fermentation time is 18-20 d; when the temperature is (15, 20), the fermentation time is 16-18 d; when the temperature is (20, 25), the fermentation time is 12 d-14 d; when the temperature is (25, 30), the fermentation time is 10-12 d; when the temperature is (30, 35), the fermentation time is 8-10 d.
In the invention, the physical properties of the fermentation broth are shown by the light yellow and clear upper liquid, no ammonia smell and mellow smell. The condition of the fermentation liquor filtration is not particularly limited, and the condition of the conventional fermentation liquor filtration is adopted.
The obtained filtrate is mixed with the composite endophyte and kept stand for 3-4 d at 20-25 ℃ to obtain the crop rhizosphere soil conditioner; the composite endophyte root fungus comprises Ming's sacculus mould, root inner sacculus mould, mortierella gemini and young Leuconostoc. In the invention, the mass ratio of the filtrate to the composite endophyte is 10-12:1. In the invention, the quantity ratio of the Ming's sacculus mildew to the root endo-rhizopus, the Mortierella moellendorffii to the young Leuconostoc is preferably 0.5-1.5:0.5-1.5, more preferably 1:1:1:1; the total spore number of the composite endophyte is preferably 70-90 spores/g. The invention preferably adsorbs the composite endophyte on the kaolin which is sieved by a 200-mesh sieve after calcination for reuse. In the invention, the composite endophyte root bacteria have the functions of improving the soil rhizosphere microenvironment and promoting root growth. In the invention, latin of the Ming saccule mould is Rhizophagus clarus, latin of the rhizopus radiatus mould is Rhizophagus intraradices, latin of the Mortierella gemini is Funneliformis mosseae, and Latin of the young Leuconostoc is Glomus etunicatum.
The invention also provides application of the crop rhizosphere soil conditioner in improving soil according to the environment.
In the present invention, the application preferably includes:
when the fertilizer is used for seedling and transplanting crops, the fertilizer is applied in combination with root setting water during field planting, 10-12 kg of rhizosphere soil conditioner for each mu of crops is diluted 2000-2500 times and sprayed, 8-10 kg of rhizosphere soil conditioner for each mu of crops is diluted 1500-2000 times after the seedlings are planted and sprayed again;
when the plant is used for direct seeding or cutting, when 1-2 leaves are arranged on seedlings (or after cutting, living plants) and watering is combined for spraying once, 8-10 kg of the rhizosphere soil conditioner for each mu of the plant is diluted by 2000-2500 times and sprayed once again after 10 days, 10-12 kg of the rhizosphere soil conditioner for each mu of the plant is diluted by 1500-2000 times;
when the fertilizer is used for paddy rice, 8-10 kg of the rhizosphere soil conditioner for each mu of seedlings is diluted by 100 times and sprayed into the field when the fertilizer is used for paddy rice, 4-6 kg of the rhizosphere soil conditioner for each mu of seedlings is diluted by 20-30 times and soaked in the root when the fertilizer is transplanted to the field, and the fertilizer is completely carried into the field along with the seedlings.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The paenibacillus polymyxa is cultured conventionally to obtain paenibacillus polymyxa bacterial liquid; the bacillus licheniformis is cultured conventionally to obtain bacillus licheniformis bacterial liquid; the bacillus laterosporus is cultured conventionally to obtain bacillus laterosporus bacterial liquid.
The prepared Paenibacillus polymyxaInoculating bacterial liquid, bacillus licheniformis bacterial liquid and Bacillus laterosporus into fermentation tank, inoculating culture medium with 5% of fermentation tank, taking fresh non-slag fecal sewage as solvent, including molasses 0.3g/L, corn meal 0.5g/L, ammonium sulfate 0.05g/L, sodium acetate 0.05g/L, magnesium sulfate 0.02g/L and potassium dihydrogen phosphate 0.02g/L, stirring at 30deg.C for 1 time every 3h for 15min, sampling to detect microorganism quantity, and collecting mixed bacteria quantity up to 10 6 cfu ml -1 Adding EM active bacterial liquid (1:1; V:V) during the process, culturing under aerobic fermentation condition, controlling the temperature at 30deg.C, stirring for 1 time every 3h for 15min each time, sampling to detect microorganism quantity, and when microorganism quantity reaches 10 9 cfu ml -1 And obtaining the composite bacterial liquid with the pH range stabilized at 4.0. The EM active bacterial liquid is formed by diluting EM stock solution and deionized water according to the volume ratio of 1:99. EM stock solution was purchased from the environmental biotechnology of the lehr, inc.
Preparing a composite endophyte microbial agent:
mixing the Ming's sacculus mould, the rhizopus radiatus, the Mortierella gemini and the young Leuconostoc mould according to the mass ratio of 1:1:1:1, and using the kaolin which is sieved by a 200-mesh sieve after calcination as an adsorption carrier, wherein the total spore number is 70-90/g.
Soybean powder in weight ratio: brown sugar: composite bacterial liquid: mixing the slag-free sewage in a ratio of 1:1:1:10, regulating the pH value to be 4, uniformly stirring, controlling the temperature to be 32 ℃, and fermenting for 8d; the physical properties of the fermentation liquor are shown as light yellow and clear upper layer liquid, no ammonia smell and mellow smell.
Filtering the fermentation liquor to obtain filtrate.
Mixing the filtrate with the composite endophyte root microbial agent according to the weight ratio of 10:1, and storing for 4 days at 25 ℃ to obtain the crop rhizosphere soil conditioner.
The detection shows that the quantity of the paenibacillus polymyxa in the crop rhizosphere soil conditioner is 4 multiplied by 10 8 cfu/mL, the number of Bacillus licheniformis is 3×10 6 cfu/mL, the number of bacillus laterosporus is 2×10 8 cfu/mL, number of Ming's saccule mold is 1×10 8 cfu/mL, the number of rhizopus in root is 2×10 8 cfu/mL, the amount of Mortierella gemini is 1.5X10 8 cfu/mL, number of young dactylotheca mesenchyma is 1.5X10 8 cfu/mL。
Example 2
Seedling raising of cabbage in 6 months and 6 days of 2019, planting in water saving garden in 18 months at university of river and sea, 400m 2 For experiments in which 100m was compared 2 The treatment group occupies 300m 2 . 10kg of prepared crop rhizosphere soil conditioner (prepared in example 1) is diluted 2000 times and sprayed in field planting, 8kg of prepared crop rhizosphere soil conditioner (prepared in example 1) is diluted 1500 times and sprayed once again after 25 days of field planting, and three times of parallel are arranged. The soil conditioner is applied proportionally according to the planting area. Control: and spraying the same amount of clean water, and planting for 45 days. The results of the growth potential measurement are shown in Table 1.
TABLE 1 results of growth potential measurement
Blade dry weight g/plant Total dry weight g/strain Leaf biomass g/plant
Control 2.08 2.42 22.89
Process 1 2.98 3.01 32.1
Process 2 3.07 3.56 35.3
Process 3 3.12 3.53 33.8
Example 3
The mass ratio of the composite bacterial liquid to the soybean powder to the brown sugar to the fecal sewage is 1:1:10, the pH value of the fermentation liquid is regulated to 4 during the preparation, the temperature is controlled to 15 ℃, the fermentation is carried out for 18 days, the soil conditioner is prepared by filtering the fermentation liquid and preparing the composite endophyte root bacterial agent according to the weight ratio of 11:1, and other methods are the same as in example 1.
Through detection, the quantity of the paenibacillus polymyxa in the crop rhizosphere soil conditioner is 5 multiplied by 10 8 cfu/mL, the number of Bacillus licheniformis is 3×10 6 cfu/mL, the number of bacillus laterosporus is 2×10 8 cfu/mL, number of Ming's saccule mold is 2×10 8 cfu/mL, the number of rhizopus in root is 2×10 8 cfu/mL, 2X 10 number of Mortierella gemmifera 8 cfu/mL, number of young dactylotheca mesenchyma is 1.5X10 8 cfu/mL。
And (3) treatment: after germination of the asparagus in 8 months and 6 days of 2019, seedling is cultivated in a water-saving garden of the university of river and sea in 18 days of 9 months, and four mu of land are used for experiments, wherein the treated group occupies three mu of land in comparison with one mu of land. 10kg of prepared crop rhizosphere soil conditioner (prepared in example 3) is diluted 2000 times and sprayed in field planting, 8kg of prepared crop rhizosphere soil conditioner (prepared in example 3) is diluted 1500 times and sprayed again after 25 days of field planting, and three times of parallel are arranged. Control: and spraying the same amount of clean water, and planting for 45 days. Growth vigor was measured 5 days after 5 months in the next year, and the results are shown in Table 2.
TABLE 2 results of growth potential measurement
Figure SMS_1
As can be seen from table 2, the difference in growth vigor is evident. The stem thickness is obviously increased after the secondary, and the new branches are also obviously increased.
Example 4
The mass ratio of the composite bacterial liquid to the soybean meal to the brown sugar to the fecal sewage is preferably 1:1:1:10. The pH of the fermentation broth is adjusted to 5 during preparation, the temperature is controlled to 20 ℃, the fermentation is carried out for 16 days, the rhizosphere soil conditioner is prepared by filtering the fermentation broth and compounding the endophyte root microbial inoculum according to the weight ratio of 12:1, and other methods are the same as in example 1.
Through detection, the quantity of the paenibacillus polymyxa in the crop rhizosphere soil conditioner is 5 multiplied by 10 8 cfu/mL, the number of Bacillus licheniformis is 5×10 6 cfu/mL, the number of bacillus laterosporus is 4 multiplied by 10 8 cfu/mL, number of Ming's saccule mold is 2×10 8 cfu/mL, the number of rhizopus in root is 2×10 8 cfu/mL, the amount of Mortierella gemini is 1.5X10 8 cfu/mL, number of young sleeve-proximal Ming sacculus mildew is 2 multiplied by 10 8 cfu/mL。
Application to broccoli
And (3) treatment: plug seedling is planted in 8 months and 10 days in 2019, water-saving garden is planted in 14 days in 9, and four mu land is used for experiments, wherein the treatment group occupies three mu in comparison with one mu. Diluting the prepared crop rhizosphere soil conditioner (prepared in example 4) by 2500 times with 12kg per mu in field planting, spraying the prepared crop rhizosphere soil conditioner (prepared in example 4) for 1 time after 10kg per mu of the prepared crop rhizosphere soil conditioner (prepared in example 4) is diluted by 2000 times 25 days after field planting, and setting three times in parallel; control: and spraying the same amount of clean water. Growth potential was measured 45 days after planting, yield was measured at harvest, and the results are shown in Table 3.
TABLE 3 growth vigor and yield results
Figure SMS_2
As can be seen from Table 3, the difference in growth vigor was remarkable, the yield was increased, and the average single-sphere weight was increased by 33%.
Example 5
The mass of the composite bacterial liquid, the soybean powder, the brown sugar and the fecal sewage is selected to be 1:1:1:10, the pH value of the fermentation liquid is regulated to be 5 during the preparation, the temperature is controlled to be 25 ℃, the fermentation is carried out for 10 days, the soil conditioner is prepared by filtering the fermentation liquid and the composite endophyte root bacterial agent according to the weight ratio of 11:1, and other conditions are the same as in example 1.
The detection shows that the quantity of the paenibacillus polymyxa in the crop rhizosphere soil conditioner is 4 multiplied by 10 8 cfu/mL, the number of Bacillus licheniformis is 5×10 6 cfu/mL, the number of bacillus laterosporus is 4 multiplied by 10 8 cfu/mL, number of Ming's saccule mold is 2×10 8 cfu/mL, the number of rhizopus in root is 3×10 8 cfu/mL, 2X 10 number of Mortierella gemmifera 8 cfu/mL, number of young sleeve-proximal Ming sacculus mildew is 2 multiplied by 10 8 cfu/mL。
Application of sweet potato in leaves
And (3) treatment: cutting at 5 months and 30 days in 2019, living at 5 days in 6 months, water-saving garden at university of river and sea, and four acres of land are used for experiments, wherein the treatment group occupies three acres compared with one acre. At this time, 9kg of prepared crop rhizosphere soil conditioner (prepared in example 5) is diluted 2200 times and sprayed every mu, and after 10 days, 11kg of prepared crop rhizosphere soil conditioner (prepared in example 5) is diluted 1700 times and sprayed 1 time more, and three times are arranged in parallel. Control: and spraying the same amount of clean water. Growth potential measurement was performed 30 days after cutting, and the results are shown in Table 4.
TABLE 4 determination of growth potential
Figure SMS_3
As can be seen from table 4, the difference in growth vigor is remarkable.
Example 6
The results of the change of physical and chemical properties of the soil after application are shown in Table 5.
In example 2, the index measurement of the planted soil was performed after two years of application, wherein the volume weight was measured by adopting a method for measuring the volume weight of NYT-1121.42006 soil, the water-stable granules were measured by adopting an electric water sieve method, the organic matters were measured by adopting a method for measuring soil organic matters in GB9834-1998 soil, the micro respiration amount of the soil was measured by adopting a laboratory method for measuring the respiration of soil microorganisms in GB32720-2016, and the measurement of the microorganism number was performed according to a method provided by soil microorganism research institute Li Fangfa, lin Guixian, higher education publishing agency, 2010 edition.
TABLE 5 variation of physical and chemical properties of soil
Figure SMS_4
As can be seen from Table 5, after two years of use, the soil volume weight was decreased, the water-stable granules were increased, the organic matter content was increased, the number of useful microorganisms in the soil was increased, the activity was enhanced, and the soil environment was remarkably improved.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (8)

1. A crop rhizosphere soil conditioner is characterized in that the quantity of paenibacillus polymyxa in the crop rhizosphere soil conditioner is 4-5 multiplied by 10 8 cfu/mL, the quantity of the bacillus licheniformis is 3 to 5 multiplied by 10 6 cfu/mL, the quantity of the bacillus laterosporus is 2-4 multiplied by 10 8 cfu/mL, the number of the Ming's sacculus mildew is 1-2 multiplied by 10 8 cfu/mL, the quantity of the rhizopus in the root is 2-3 multiplied by 10 8 cfu/mL, the quantity of the Mortierella gemmifera is 1.5-2 multiplied by 10 8 cfu/mL, the number of young sleeve-type Ming's sacculus mildew is 1.5-2 multiplied by 10 8 cfu/mL；
The preparation method of the crop rhizosphere soil conditioner comprises the following steps:
1) Inoculating Paenibacillus polymyxa, bacillus licheniformis and Bacillus laterosporus into propagation medium, and culturing until the total microorganism content in the culture solution is 2×10 9 When cfu/mL is more than or equal to the cfu/mL, mixing the culture solution with EM bacterial solution, and performing aerobic fermentation to obtain a complexThe total content of microorganisms in the composite bacterial liquid is 2.2x10 9 cfu/mL or more;
2) Mixing the composite bacterial liquid obtained in the step 1), soybean powder, brown sugar and fecal sewage, fermenting for 8-20 d at 10-35 ℃ to obtain fermentation liquor, and filtering the fermentation liquor to obtain filtrate;
3) Mixing the filtrate obtained in the step 2) with composite endophyte, and standing for 3-4 d at 20-25 ℃ to obtain a crop rhizosphere soil conditioner;
the composite endophyte root fungus comprises Ming's sacculus mould, root inner sacculus mould, mortierella gemini and young Leuconostoc.
2. The crop rhizosphere soil conditioner according to claim 1, wherein the propagation medium in step 1) uses slag-free waste water as a solvent, and each liter of the propagation medium comprises 0.2-0.3 g/L molasses, 0.3-0.5 g/L corn meal, 0.05-0.1 g/L ammonium sulfate, 0.05-0.08 g/L sodium acetate, 0.01-0.02 g/L magnesium sulfate and 0.01-0.03 g/L potassium dihydrogen phosphate.
3. The crop rhizosphere soil conditioner of claim 1, wherein the conditions of step 1) cultivation and aerobic fermentation respectively comprise: the temperature is 30-35 ℃, and the stirring is carried out for 1 time every 2-3 hours for 10-15 min each time.
4. The crop rhizosphere soil conditioner according to claim 1, wherein the volume ratio of the culture solution in step 1) to the EM bacterial solution is 0.5-1.5:0.5-1.5;
the preparation method of the EM bacterial liquid comprises the following steps: and mixing the EM bacteria stock solution with deionized water according to the volume ratio of 1:99.
5. The crop rhizosphere soil conditioner according to claim 1, wherein the mass ratio of the compound bacterial liquid, the soybean meal, the brown sugar and the fecal sewage in the step 2) is 0.5-1.5:0.5-1.5:8-12; the fecal sewage is slag-free fecal sewage.
6. The crop rhizosphere soil conditioner of claim 1, wherein the mass ratio of the filtrate of step 3) to the complex endophyte is 10-12:1.
7. The crop rhizosphere soil conditioner of claim 1, wherein the spore count ratio of said Ming's saccule mold, rhizopus radiatus, fabricius mosaicus and young sleeve Ming's saccule mold is 0.5-1.5:0.5-1.5;
the total spore number of the composite endophyte is 70-90/g.
8. Use of the crop rhizosphere soil conditioner of claim 1 for improving the soil root system environment.
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