CN101898915B - Microbial agent suitable for crops in seedling stage and preparation method thereof - Google Patents
Microbial agent suitable for crops in seedling stage and preparation method thereof Download PDFInfo
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- CN101898915B CN101898915B CN 200910084799 CN200910084799A CN101898915B CN 101898915 B CN101898915 B CN 101898915B CN 200910084799 CN200910084799 CN 200910084799 CN 200910084799 A CN200910084799 A CN 200910084799A CN 101898915 B CN101898915 B CN 101898915B
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- microbiobacterial agent
- bacteria
- bacillus
- seedling stage
- solid fungicide
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- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 230000000813 microbial effect Effects 0.000 title abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 46
- 241000894006 Bacteria Species 0.000 claims abstract description 43
- 239000007787 solid Substances 0.000 claims abstract description 26
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 7
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 6
- 239000011573 trace mineral Substances 0.000 claims abstract description 5
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 5
- 230000000855 fungicidal effect Effects 0.000 claims description 20
- 239000000417 fungicide Substances 0.000 claims description 20
- 239000002131 composite material Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000003415 peat Substances 0.000 claims description 17
- 241000195940 Bryophyta Species 0.000 claims description 15
- 230000001580 bacterial effect Effects 0.000 claims description 13
- 241000187395 Streptomyces microflavus Species 0.000 claims description 10
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 10
- 239000004575 stone Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 239000003337 fertilizer Substances 0.000 claims description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 229910021538 borax Inorganic materials 0.000 claims description 6
- QYHFIVBSNOWOCQ-UHFFFAOYSA-N selenic acid Chemical compound O[Se](O)(=O)=O QYHFIVBSNOWOCQ-UHFFFAOYSA-N 0.000 claims description 6
- 239000004328 sodium tetraborate Substances 0.000 claims description 6
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 6
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 241000193752 Bacillus circulans Species 0.000 claims description 4
- 241000193747 Bacillus firmus Species 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- 241000193386 Lysinibacillus sphaericus Species 0.000 claims description 4
- 229940005348 bacillus firmus Drugs 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 4
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- 229960001763 zinc sulfate Drugs 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 238000009461 vacuum packaging Methods 0.000 claims description 3
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical group [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052796 boron Inorganic materials 0.000 claims description 2
- 238000007796 conventional method Methods 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 238000005138 cryopreservation Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 229910052711 selenium Inorganic materials 0.000 claims description 2
- 239000011669 selenium Substances 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 244000005700 microbiome Species 0.000 abstract description 12
- 230000009286 beneficial effect Effects 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 6
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- 239000002207 metabolite Substances 0.000 abstract description 5
- 230000007226 seed germination Effects 0.000 abstract description 5
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 4
- 229910052700 potassium Inorganic materials 0.000 abstract description 4
- 230000014284 seed dormancy process Effects 0.000 abstract description 4
- 241000186361 Actinobacteria <class> Species 0.000 abstract description 3
- 229930191978 Gibberellin Natural products 0.000 abstract description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 3
- 239000003448 gibberellin Substances 0.000 abstract description 3
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 abstract description 3
- 239000011574 phosphorus Substances 0.000 abstract description 3
- 239000011591 potassium Substances 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 102000018997 Growth Hormone Human genes 0.000 abstract description 2
- 108010051696 Growth Hormone Proteins 0.000 abstract description 2
- 239000000122 growth hormone Substances 0.000 abstract description 2
- 230000021749 root development Effects 0.000 abstract description 2
- 241000235342 Saccharomycetes Species 0.000 abstract 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract 1
- 239000004062 cytokinin Substances 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000004310 lactic acid Substances 0.000 abstract 1
- 239000005648 plant growth regulator Substances 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 17
- 239000002068 microbial inoculum Substances 0.000 description 8
- 239000000843 powder Substances 0.000 description 7
- YNWVFADWVLCOPU-MDWZMJQESA-N (1E)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol Chemical compound C1=NC=NN1/C(C(O)C(C)(C)C)=C/C1=CC=C(Cl)C=C1 YNWVFADWVLCOPU-MDWZMJQESA-N 0.000 description 6
- 230000001939 inductive effect Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
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- 239000000126 substance Substances 0.000 description 5
- 238000012545 processing Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 244000291564 Allium cepa Species 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 239000010815 organic waste Substances 0.000 description 3
- 239000003375 plant hormone Substances 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 235000010167 Allium cepa var aggregatum Nutrition 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
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- 244000000000 soil microbiome Species 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 2
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
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- 244000037666 field crops Species 0.000 description 1
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- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
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- 229910000155 iron(II) phosphate Inorganic materials 0.000 description 1
- SDEKDNPYZOERBP-UHFFFAOYSA-H iron(ii) phosphate Chemical compound [Fe+2].[Fe+2].[Fe+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O SDEKDNPYZOERBP-UHFFFAOYSA-H 0.000 description 1
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Landscapes
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a microbial agent suitable for crops in the seedling stage. The microbial agent is prepared by taking actinomycetes, bacillus, nitrogen-fixing bacteria, phosphorus bacteria, potassium bacteria, lactic acid bacteria, saccharomycetes, other beneficial microorganisms and metabolites thereof as the main components and adding a certain proportion of trace elements favorable for the growth of crops in the seedling stage. Owing to solid fermentation in the preparation process of the microbial agent, the product contains a large number of beneficial microorganisms, furthermore, the metabolites of the microorganisms are rich in various plant growth regulators, such as growth hormone, cytokinin, gibberellins and the like, thus achieving the obvious effects of breaking the seed dormancy, promoting the seed germination, increasing the emergence rate and promoting the root development.
Description
Technical field
The present invention relates to a kind of microbial fertilizer and preparation method thereof, particularly relate to a kind of microbiobacterial agent that is suitable for the crops seedling stage use and preparation method thereof.
Background technology
The applicant is devoted to the research and development utilization of compound microbial culture starter for a long time, it is 200610083429.7 that the applicant has submitted application number on May 31st, 2006, name is called the Chinese patent application of " adopting composite bacteria that dining-kitchen organic waste is carried out the method that resources circulation is processed ", this patent of invention is now obtained the authorization, a kind of composite bacteria is wherein disclosed, it contains following bacterial classification: Bacillus circulans, Bacillus sphaericus, bacillus firmus, the Amur genus bacillus, bacstearothermophilus, subtilis, yeast, milk-acid bacteria, each bacterial classification can be individually according to a conventional method from the bacterial classification of cryopreservation the streak inoculation of picking bacterial classification to the solid plate substratum, under conditions suitable, cultivate, bacterium colony to be grown, be inoculated in liquid nutrient medium and carry out shaking culture, carry out again as required enlarged culturing; After each bacterial classification of above-mentioned cultivation can liquid form be cultivated, ratio in the viable count of Bacillus circulans, Bacillus sphaericus, bacillus firmus, Amur genus bacillus, bacstearothermophilus, subtilis, yeast, milk-acid bacteria: (0.8-1.1): (1.2-1.5): (1-1.2): (1.4-1.6): (2.2-3): (1.2-1.5): (1-1.2): (0.8-1.2), be mixed and made into liquid fermentation agent, save backup in 4 ℃; Also can preserve separately instant mixing, the instant use; Also the liquid culture lyophilize can be obtained solid bacterium powder, make the fermenting agent of solid form.The applicant finds that this composite bacteria can produce plurality of enzymes such as proteolytic enzyme, lipase, amylase, chitinase, can be micromolecular polypeptide and oligosaccharides with the high molecular weight protein in the organism and fat acid decomposition, aforementioned patent only is used in it on fermentative processing of dining-kitchen organic waste, the continuous ground further investigation of applicant finds that this composite bacteria its metabolic activity and meta-bolites in metabolic process also have good promoter action to the growth of plant crop, continue to have proposed the application on this basis.
Summary of the invention
The application quoted the patent No. be 200610083429.7 (publication number CN1850965A) Chinese invention patent full text as a reference.
The purpose of this invention is to provide and a kind ofly be suitable for crop by being seeded into the microbiobacterial agent that seedling stage, process was used, it can break seed dormancy, promotes seed germination, promotes root system development, improves the rice shoot resistance, improves the nursery soil environment, for rice shoot provides adequate nutrients.
A kind of microbiobacterial agent that is suitable for the crops seedling stage use, its following component by weight percent forms:
Composite bacteria solid fungicide: 50%～70%;
The peat composed of rotten mosses: 5%～15%;
Streptomyces microflavus solid fungicide: 10%～20%;
Medical stone: 8%～12%;
Acceptable micro-inorganic salt: 1%～3%;
Described composite bacteria is that the patent No. is the published composite bacteria that is suitable for dining-kitchen organic waste is carried out fermentative processing in 200610083429.7 the Chinese patent, and described composite bacteria solid fungicide and streptomyces microflavus solid fungicide are the products of spawn culture postlyophilization;
Living bacteria count in the described microbiobacterial agent 〉=200,000,000/gram.
Microbiobacterial agent of the present invention, it preferably is comprised of the following component of weight percent:
Composite bacteria solid fungicide: 60%;
The peat composed of rotten mosses: 13%;
Streptomyces microflavus solid fungicide: 15%;
Medical stone: 10%;
Acceptable micro-inorganic salt: 2%.
Microbiobacterial agent of the present invention, its water content is less than or equal to 12.0 % by weight, and organic carbon content is more than or equal to 47 % by weight.
Microbiobacterial agent of the present invention, wherein the peat composed of rotten mosses is being commonly called as of meadow peat, is before 5,000 and even 10,000 years on the bottom land, plant is withered year after year, is half state that rots, and piles up year by year the organic ore body that forms.The peat composed of rotten mosses has water-retentivity, and ventilation property can be as good microbe carrier, and has the effect of improvement soil.The peat composed of rotten mosses contains abundant humic acids, is the organism of pure natural, and is nontoxic aseptic.The peat composed of rotten mosses also contains abundant nitrogen, phosphorus, potassium etc.Use Peat substitution part complex bacteria culture, can reduce the part producing cost.Medical stone mainly is used as carrier and some trace elements is provided, and possesses about soil improvement simultaneously.The production of this microbiobacterial agent also needs additionally to add the required trace element of plant-growth, such as zinc, iron, selenium, copper and boron etc.It can add in the microbial inoculum by the acceptable inorganic salt form of plant, such as zinc sulfate 0.5%, ferric sulfate 0.5%, inclined to one side selenic acid 0.4%, copper sulfate 0.3% and borax 0.3% etc.
The preparation method of microbiobacterial agent of the present invention mixes the final vacuum packing in proportion after described component pulverized.Finished state is powdery, and fineness reaches 80 orders, and water content is less than or equal to 12.0 % by weight, living bacteria count 〉=200,000,000/gram wherein, and organic carbon content is more than or equal to 47 % by weight.
Microbiobacterial agent of the present invention, can be with reference to following using method during use:
(1) seedbed is spread fertilizer over the fields or directly dressed seed, soaks seed: 500g evenly spreads fertilizer over the fields in 15m with this product
2The seedbed.The Different Crop kind is used by existing method when seed dressing, seed soaking.
(2) cave dish and feeding block seedlings raising: mix Nutrition Soil or matrix 100～120kg with this product 500g, evenly fill seeding and seedling raising behind the alms bowl.
(3) water seedling stage and execute: at crops seedling stage, convert clear water 45kg with this product 500g at every turn and evenly spray or convert clear water 100kg and water and impose on the seedbed, the interval was used in conjunction 2～3 times in 7 days.
(4) before transplanting seedling stage, use: dip in root convert clear water 5kg transplanting with this product 500g before, perhaps convert clear water 100kg with this product 500g and water and impose on the seedbed.
The microbiobacterial agent that is suitable for the crops seedling stage use of the present invention has following beneficial effect:
(1) breaks seed dormancy, promote seed germination.Use this microbiobacterial agent during seed dressing, therein in the beneficial microorganism vital movement process, produce some meta-bolitess and induce Interior Seed germination material, such as some hormones such as indolylacetic acid and its derivative and Plant hormones regulators,gibberellins, strengthen the respiratory metabolism of seed, strengthen the activity of enzyme, make the nutritive substance running of storage in the seed, embryo section cell begins division, elongation, and radicle and plumular axis begin growth, prominent breaking in the seed coat.
(2) promote root growth and development, fibrous root is flourishing.This microbiobacterial agent is by the improvement soil physical and chemical property, for good soil nutrient and microbial environment have been created in the growth of plant root to the effect of seedlings root growth on the one hand; Be that the beneficial microorganism meta-bolites has the plant hormone function on the other hand, stimulate crop root to grow to realize.
(3) make that the crop seedling is strong, blade is loose dark green, promote plant growth.Secrete organic acid with the beneficial microorganism after nursery soil cooperates, the Nutrient Transformation that accelerates in the medium of seedling bed is the form that crop directly absorbs, and replenishes rapidly the required various elements of crop, promotes plant growth.
(4) improve seedling quality, strengthen crop anti-adversity.In seed dressing period, this microbiobacterial agent surrounds seed-coat, forms dominant microflora, with pathogenic bacteria contention available stock, thereby suppresses growing of pathogenic bacteria, eradicates in the early stage issuable disease of later stage.
(5) seedling-slowing stage is short, and it is vigorous to grow.Before Seeding planting, be transplanted into the land for growing field crops after can using this microbiobacterial agent to dip in root, microbial bacteria is known from experience ramp, induces early Fa Xingen of plant, has shortened transplanting seedling time.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
Embodiment 1: a kind of microbiobacterial agent that is suitable for the crops seedling stage use, and its following component by weight percent forms:
Composite bacteria solid fungicide: 60%;
The peat composed of rotten mosses: 13%;
Streptomyces microflavus solid fungicide: 15%;
Medical stone: 10%;
Acceptable micro-inorganic salt: 2%, wherein zinc sulfate 0.5%, ferric sulfate 0.5%, partially selenic acid 0.4%, copper sulfate 0.3% and borax 0.3%.
Mix in proportion the final vacuum packing after said components pulverized.Finished state is powdery, and fineness reaches 80 orders, and water content is less than or equal to 12.0 % by weight, living bacteria count 〉=200,000,000/gram wherein, and organic carbon content is more than or equal to 47 % by weight.
Embodiment 2: except the amounts of components that forms microbiobacterial agent was distinguished to some extent, all the other were all identical with embodiment 1, and described component and consumption are:
Composite bacteria solid fungicide: 50%;
The peat composed of rotten mosses: 15%;
Streptomyces microflavus solid fungicide: 20%;
Medical stone: 12%;
Acceptable micro-inorganic salt: 3%, wherein zinc chloride 0.6%, iron protochloride 0.6%, partially selenic acid 0.6%, cupric chloride 0.6% and borax 0.6%.
Embodiment 3: except the amounts of components that forms microbiobacterial agent was distinguished to some extent, all the other were all identical with embodiment 1, and described component and consumption are:
Composite bacteria solid fungicide: 70%;
The peat composed of rotten mosses: 5%;
Streptomyces microflavus solid fungicide: 16%;
Medical stone: 8%;
Acceptable micro-inorganic salt: 1%, wherein zinc phosphate 0.2%, ferrous phosphate 0.2%, partially selenic acid 0.2%, copper sulfate 0.2% and borax 0.2%.
Embodiment 4: except the amounts of components that forms microbiobacterial agent was distinguished to some extent, all the other were all identical with embodiment 1, and described component and consumption are:
Composite bacteria solid fungicide: 65%;
The peat composed of rotten mosses: 13%;
Streptomyces microflavus solid fungicide: 10%;
Medical stone: 10%;
Acceptable micro-inorganic salt: 2%, wherein zinc sulfate 0.5%, ferric sulfate 0.5%, partially selenic acid 0.4%, copper sulfate 0.3% and borax 0.3%.。
Test example: manure trial
Green house of vegetables has carried out the fertilizer test of microbiobacterial agent on shallot of embodiment 1～4 in Beijing Da Dong stream vegetables, strawberry cultivating base.Experimental field soil is light loamy texture cinnamon soil, 10 mu of test area, and test is " long pleasing " with green onion breed.
For trying fertilizer: present embodiment microbiobacterial agent, uniconazole, root-inducing powder and clear water.
Test method:
(1) uniconazole: seed with 10mg/L times of liquid seed soaking of uniconazole 12 hours, is mixed the peat composed of rotten mosses (500g/10m
2) be sown in the soil.
(2) root-inducing powder: seed with 30mg/L times of liquid seed soaking of root-inducing powder 12 hours, is mixed the peat composed of rotten mosses (500g/10m
2) be sown in the soil.
(3) microbiobacterial agent: seed was soaked seed 12 hours mixing microorganisms microbial inoculum (500g/10m with pure water
2) be sown in the soil.
(4) clear water: seed with pure water seed soaking 12 hours, is mixed the peat composed of rotten mosses (500g/10m
2) be sown in the soil.
Seedling stage, (after planting 20 days) were measured shallot overground part stem height.
Test-results sees the following form:
| The processing project | Clear water | Uniconazole | Root-inducing powder | Embodiment 1 microbial inoculum | Embodiment 2 microbial inoculums | Embodiment 3 microbial inoculums | Embodiment 4 microbial inoculums |
| Overground part stem long (mean value) | 5.25 cm | 5.9 cm | 6.49 cm | 6.68 cm | 6.55 cm | 6.59 cm | 6.62 cm |
Test-results shows that the stem length that microbiobacterial agent is processed than clear water, uniconazole and root-inducing powder is improved respectively, and the microbiobacterial agent of embodiment 1 improves at most, the long raising 27.2%, 13.2%, 2.9% of stem of processing than clear water, uniconazole and root-inducing powder respectively.
As can be seen from the above embodiments, the embodiment of the invention is the chief component material by adopting the multiple beneficial microorganism such as actinomycetes, genus bacillus, vinelandii, phosphorus bacteria, potassium bacterium, milk-acid bacteria, yeast and meta-bolites thereof, and added a certain proportion of trace element of suitable crops seedling stage growth, make microbiobacterial agent, pass through solid fermentation in the use procedure, so that not only contain a large amount of beneficial microorganisms in the product, and be rich in the various plants growth regulatory substance.Its activity substance content is 10～100 times of general bacterial fertilizer.The various plants growth substances such as the growth hormone that microbial inoculum produces during the fermentation, phytokinin, Plant hormones regulators,gibberellins, as the adjusting control agent of crop growth (especially seed, rice shoot), to breaking seed dormancy, promote seed germination, improve seedling rate, promoting that root system development plays a significant role.Microbial inoculum has improved the wherein quantity of beneficial functions bacterium after being manured into soil, promoted conversion and the release of solubility N, P, K in the organic substance, and the enrichment of growth promotion metabolic active substance.
The selected microorganism strains of this product all derives from soil or commodity purchasing.The microbiobacterial agent finished product that the present invention is suitable for the crops seedling stage use has following characteristic:
(1) soil bacteria: being comprised of tens kinds of bacterial classifications such as dominant bacteria sporeformer, actinomycetes, milk-acid bacteria, yeast, is the composite microbial bacteria that derives from soil, the one-level safety standards.
(2) high-temperature aerobic bacterium: low to environmental requirement, homogeneity of product is strong, but commercial application.
(3) fermentation finished thoroughly1: in the fermentation process, animal protein and vegetable protein all are converted into tropina, form reconstituted product.Simultaneously, effectively kill various harmful pathogenic bacterias and virus;
(4) high-energy: microbiobacterial agent had both had the viable bacteria function from taking high energy, had again trophic function, the economic benefits and social benefits combination.
(5) high-level efficiency: beneficial microorganism becomes dormant state with the form of gemma under standard state, stable existence, and the rear horse back propagation that is manured into soil, with the soil bacteria synergy, product application efficient is very high.
(6) stability is strong: the beneficial microorganism rate of decay is slow, and effect is stable.Bacterial classification is without the indoor cultivation of going down to posterity, and vitality is strong.
The above only is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. one kind is suitable for the microbiobacterial agent that crops seedling stage uses, and it is characterized in that its following component by weight percent forms:
Composite bacteria solid fungicide: 50% ~ 70%;
The peat composed of rotten mosses: 5% ~ 15%;
Streptomyces microflavus solid fungicide: 10% ~ 20%;
Medical stone: 8% ~ 12%;
Acceptable micro-inorganic salt: 1% ~ 3%;
Described composite bacteria contains following bacterial classification: Bacillus circulans, Bacillus sphaericus, bacillus firmus, Amur genus bacillus, bacstearothermophilus, subtilis, yeast, milk-acid bacteria; Each bacterial classification individually according to a conventional method from the bacterial classification of cryopreservation the streak inoculation of picking bacterial classification cultivate under conditions suitable to the solid plate substratum, bacterium colony to be grown is inoculated in liquid nutrient medium and carries out shaking culture, carries out as required enlarged culturing again; After each bacterial classification of above-mentioned cultivation is cultivated with liquid form, ratio in the viable count of Bacillus circulans, Bacillus sphaericus, bacillus firmus, Amur genus bacillus, bacstearothermophilus, subtilis, yeast, milk-acid bacteria: (0.8-1.1): (1.2-1.5): (1-1.2): (1.4-1.6): (2.2-3): (1.2-1.5): (1-1.2): (0.8-1.2), be mixed and made into liquid fermentation agent, save backup in 4 ℃; Perhaps preserve separately instant mixing, the instant use;
Described composite bacteria solid fungicide and streptomyces microflavus solid fungicide are products dry behind the spawn culture;
Living bacteria count in the described microbiobacterial agent 〉=200,000,000/gram.
2. microbiobacterial agent as claimed in claim 1 is characterized in that, its following component by weight percent forms:
Composite bacteria solid fungicide: 60%;
The peat composed of rotten mosses: 13%;
Streptomyces microflavus solid fungicide: 15%;
Medical stone: 10%;
Acceptable micro-inorganic salt: 2%.
3. microbiobacterial agent as claimed in claim 1 or 2 is characterized in that, described microbiobacterial agent water content is less than or equal to 12.0 % by weight, and organic carbon content is more than or equal to 47 % by weight.
4. microbiobacterial agent as claimed in claim 1 or 2 is characterized in that, described trace element is zinc, iron, selenium, copper and boron.
5. microbiobacterial agent as claimed in claim 4 is characterized in that, described micro-inorganic salt are zinc sulfate 0.5%, ferric sulfate 0.5%, inclined to one side selenic acid 0.4%, copper sulfate 0.3% and borax 0.3%.
6. the preparation method of microbiobacterial agent claimed in claim 1 is characterized in that, mixes in proportion the final vacuum packing after described component is pulverized.
7. the application of microbiobacterial agent claimed in claim 1 when crops seedling stage.
8. application as claimed in claim 7, described microbiobacterial agent are spread fertilizer over the fields or are watered and impose on seedbed or directly seed dressing, seed soaking.
9. application as claimed in claim 7, described microbiobacterial agent is applied to crop root before crops seedling stage is transplanted.
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