CN102115347A - Mixture fertilizer and preparation method thereof - Google Patents
Mixture fertilizer and preparation method thereof Download PDFInfo
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- CN102115347A CN102115347A CN2010105908132A CN201010590813A CN102115347A CN 102115347 A CN102115347 A CN 102115347A CN 2010105908132 A CN2010105908132 A CN 2010105908132A CN 201010590813 A CN201010590813 A CN 201010590813A CN 102115347 A CN102115347 A CN 102115347A
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- bacillus cereus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a microbial fertilizer, comprising 10-30 percent of Bacillus cereus culture, 10-30 percent of Bacillus subtilis culture, 10-30 percent of Pantoea agglomerans culture, 10-30 percent of Lactobacillus plantarum culture and 10-30 percent of Lactobacillus acidophilus culture. The microbial fertilizer can be used for soaking seeds, irrigating roots and spraying or can be used for fermenting livestock manure and a pulverized straw mixture. The microbial fertilizer also can be prepared by mixing the liquid inoculants with a well-known carrier and then drying to form the solid microbial fertilizer which is convenient to store and transport. The carrier can be bran, crop straw or other carriers suitable to be used as fertilizers.
Description
Technical field
The present invention relates to a kind of microbial fertilizer and preparation method thereof, belong to agricultural technology field.
Background technology
In order to solve or to slow down and use the pollution problem that chemical fertilizer brought, many in the world national development bio-feritlizers, microbial fertilizer can utilize the effect of vitality and the meta-bolites thereof of microorganism, improve the crop nutrition content supply, provide nutritive element, growth substance to farm crop, regulate and control its growth, reach and improve output and improve quality, the use of minimizing chemical fertilizer, the fertility that improves soil reduces and reduces the generation of disease and pest, improves the purpose of environmental quality.
Though occurred some complex micro organism fungicides in the market, but itself or function singleness, perhaps its application process is restricted, crop yield effect after the use can't reach satisfactory, so still need to develop at present complex micro organism fungicide or the fertilizer that is suitable for multiple route of administration, has comprehensive function and good effect of increasing production.
Summary of the invention
The problem to be solved in the present invention is at above problem, provide a kind of have improve soil quality, avoid soil-borne disease, reduce fertilizer amount, improve mixing microorganisms fertilizer of quality of agricultural product and output comprehensive function and preparation method thereof.
For overcoming the above problems, the technical solution used in the present invention is as follows: a kind of microbial fertilizer is characterized in that: described microbial fertilizer comprises following components by weight ratio:
Bacillus cereus culture 10～30%, Bacillus subtillis culture 10～30%, pantoea agglomerans culture 10～30%, plant lactobacillus culture 10～30% and lactobacillus acidophilus cultures 10～30%.
As further improvement in the technical proposal:
Described microbial fertilizer comprises following components by weight ratio:
Bacillus cereus culture 20%, Bacillus subtillis culture 20%, pantoea agglomerans culture 20%, plant lactobacillus culture 20% and lactobacillus acidophilus cultures 20%.
Described Bacillus cereus culture is single Bacillus cereus strain culture.
Described Bacillus cereus culture is the mixture of two or more Bacillus cereus strain cultures.
Described Bacillus cereus culture is Bacillus cereus BS8641, with the mixture of Bacillus cereus AS2015 strain culture.
The weight ratio of described Bacillus cereus BS8641 and Bacillus cereus AS2015 strain culture is 1: 1.
Described preparation method comprises the steps:
A, preparation Bacillus cereus and Bacillus subtillis fermented liquid: Bacillus cereus and Bacillus subtillis bacterial strain seed liquor are inserted in the culture medium after sterilization in 1: 1 ratio, substratum comprises that weight ratio is the bean sprouts juice of 5-10%, the glucose of 0.2-0.5%, the peptone of 0.5-1.5%, surplus is the water as solvent, inoculum size is 0.3%, be under the condition of 7.2-8.5 aerobic fermentation 16-28 hour at 32 ℃-36 ℃, PH, put jar, make Bacillus cereus and Bacillus subtillis fermented liquid;
B, the agglomerating general fermented liquid of preparation: the pantoea agglomerans seed liquor is inserted in the culture medium after sterilization, substratum comprises that weight ratio is the soyflour of 1%-3%, the Semen Maydis powder of 1%-2%, the yeast extract paste of 0.1-0.5%, the white sugar of 1-3%, the monosodium glutamate of 0.1-0.3%, the potassium primary phosphate of 0.1-0.3%, the lime carbonate of 1-3% and the sodium-chlor of 0.3-0.6%, surplus is the water as solvent, inoculum size is 0.3%, at 30-35 ℃, PH is under the 7.2-8.5 aerated culture 36-48 hour, put jar, obtain the pantoea agglomerans fermented liquid;
C, preparation Lactobacterium acidophilum and plant lactobacillus fermented liquid: Lactobacterium acidophilum and lactobacterium plantarum strain seed liquor liquid are inserted in the culture medium after sterilization in 1: 1 ratio, substratum comprises that weight ratio is the glucose of 1%-3%, the peptone of 0.5-1.5% and the yeast extract of 0.5-1%, surplus is the water as solvent, inoculum size is 0.3%, behind 32 ℃ of-36 ℃ of following anaerobically fermentings, obtain Lactobacterium acidophilum and plant lactobacillus mixed fermentation liquid fermented liquid.
D, the culture fermentation broth that obtains is made microbial fertilizer according to above-mentioned weight proportion.
Microbial fertilizer of the present invention is the direct blended liquid bacterial agent of each strain fermentation culture preferably, and it can be by seed soaking, irritate root, spray or utilize the stalk mixture of its fermentation fowl and animal excrement and pulverizing.Microbial fertilizer of the present invention also can be by mixing described liquid bacterial agent with known carrier, drying is made solid microbe fertilizer then, is convenient to store and transportation.Described carrier for example is bran, agricultural crop straw or other carrier that is suitable for use as fertilizer.
Invention microbial fertilizer of the present invention also can adopt above-mentioned bacterial classification to use the method for solid fermentation to prepare, and this class preparation method is the common practise of this area.
The present invention adopts above technical scheme, has following effect:
(1) adopt Bacillus cereus, Bacillus subtillis, Lactobacterium acidophilum and plant lactobacillus to carry out compound, by the method use of soaking seed, irritating root or spraying, can promote plant rhizosphere growth, breeding, improve the soil, eliminate and harden, water-permeable and air permeable, impel well developed root system; Potassium decomposing, phosphorus decomposing, fixed nitrogen improve fertilizer efficiency and reduce fertilizer amount; Suppress harmful bacterium breeding in the soil, the generation of various diseases such as prevention soil-borne disease, continuous cropping; Realization increases income, improves the effect of quality of agricultural product.
(2) growth characteristic that adopts single culture batch fermentation and multiple bacteria compound fermentation technology can make full use of each bacterial classification reduces the beneficial microorganism that fermentation times obtains maximum quantity, reduces production costs.
(3) utilize the nitrogen fixation of genus bacillus and pantoea agglomerans can help the effective fixed nitrogen of plant to utilize nitrogenous source in the soil from many aspects, promote plant-growth, increasing both production and income.
(4) this microbial inoculum mainly utilizes and multiplely has vinelandii effects genus bacillus as producing bacterial classification, can ensure the living bacteria count amount of product, prolongs the quality guarantee period and the shelf-lives of product.
(5) viable count in this microbial inoculum reaches 10～3,000,000,000/milliliter.
(6) starting material of biotechnological formulation of the present invention are wide material sources, with low cost, and can produce on a large scale, and product cost is low, effective, the biological safety height, fermentation period is short, fungicide preparation technology is simple, has the possibility of suitability for industrialized production, is suitable for and popularizes and promote.
The present invention is described in detail below in conjunction with embodiment.
Embodiment
Embodiment 1, prepares strain fermentating liquid as follows:
A, preparation Bacillus cereus and Bacillus subtillis fermented liquid: Bacillus cereus and Bacillus subtillis bacterial strain seed liquor are inserted in the culture medium after sterilization in 1: 1 ratio, substratum comprises that weight ratio is 5% bean sprouts juice, 0.5% glucose, 1% peptone, surplus is the water as solvent, inoculum size is 0.3%, it at 32 ℃, PH aerobic fermentation 24 hours under 7.2 the condition, put jar, make Bacillus cereus and Bacillus subtillis fermented liquid;
B, the agglomerating general fermented liquid of preparation: the pantoea agglomerans seed liquor is inserted in the culture medium after sterilization, substratum comprises that weight ratio is 2% soyflour, 1% Semen Maydis powder, 0.5% yeast extract paste, 1% white sugar, 0.1% monosodium glutamate, 0.2% potassium primary phosphate, 1% lime carbonate and 0.5% sodium-chlor, surplus is the water as solvent, inoculum size is 0.3%, at 32 ℃, PH is 7.2 times aerated culture 48 hours, put jar, obtain the pantoea agglomerans fermented liquid;
C, preparation Lactobacterium acidophilum and plant lactobacillus fermented liquid: Lactobacterium acidophilum and lactobacterium plantarum strain seed liquor liquid are inserted in the culture medium after sterilization in 1: 1 ratio, substratum comprises that weight ratio is 2% glucose, 1% peptone and 0.5% yeast extract, surplus is the water as solvent, inoculum size is 0.3%, behind 37 ℃ of following anaerobically fermentings, obtain Lactobacterium acidophilum and plant lactobacillus mixed fermentation liquid fermented liquid.
Embodiment 2, prepare strain fermentating liquid as follows:
A, preparation Bacillus cereus and Bacillus subtillis fermented liquid: Bacillus cereus BS8641, Bacillus cereus AS2015 and Bacillus subtillis K-M (Bacillus subtilis) seed liquor are inserted in the culture medium after sterilization, substratum comprises that weight ratio is 8% bean sprouts juice, 0.3% glucose and 1.05% peptone, surplus is the water as solvent, inoculum size is 0.3%, aerobic fermentation is after 24 hours under 34 ℃, the condition of PH7.2, put jar, get Bacillus cereus Bacillus subtillis mixed fermentation liquid respectively;
B, the agglomerating general fermented liquid of preparation: pantoea agglomerans WLL (Pantoea agglomerans) seed liquor is inserted in the culture medium after sterilization, substratum comprises that weight ratio is 2% soyflour, 1.5% Semen Maydis powder, 0.3% yeast extract paste, 2% white sugar, 0.2% monosodium glutamate, 0.2% potassium primary phosphate, 2% lime carbonate, 0.4% sodium-chlor, surplus is the water as solvent, inoculum size is 0.3%, at 33 ℃, p H is 7.5 times aerated culture 39 hours, put jar, obtain the pantoea agglomerans fermented liquid;
C, preparation Lactobacterium acidophilum and plant lactobacillus fermented liquid: Lactobacterium acidophilum and lactobacterium plantarum strain seed liquor liquid are inserted in the culture medium after sterilization in 1: 1 ratio, substratum comprises that weight ratio is 2% glucose, 1% peptone and 0.5% yeast extract, surplus is the water as solvent, inoculum size is 0.3%, behind 37 ℃ of following anaerobically fermentings, obtain Lactobacterium acidophilum and plant lactobacillus mixed fermentation liquid fermented liquid.
Embodiment 3, and the fermented liquid with Bacillus cereus, Bacillus subtillis, pantoea agglomerans, Lactobacterium acidophilum and the plant lactobacillus of embodiment 1 preparation mixes by following mixing ratio by weight:
Bacillus cereus BS8641, Bacillus cereus AS2015 and Bacillus subtillis K-M account for 20%, pantoea agglomerans WLL accounts for 20%, plant lactobacillus and Lactobacterium acidophilum accounts for 40%, microbial fertilizer of the present invention is made in packing then.
Embodiment 4, and the fermented liquid with Bacillus cereus, Bacillus subtillis, pantoea agglomerans, Lactobacterium acidophilum and the plant lactobacillus of embodiment 2 preparation mixes by following mixing ratio by weight:
Bacillus cereus BS8641 and Bacillus cereus AS2015 account for 20%, Bacillus subtillis K-M accounts for 20%, pantoea agglomerans WLL accounts for 20%, Lactobacterium acidophilum account for 20% and plant lactobacillus account for 20%, microbial fertilizer of the present invention is made in packing then.
Embodiment 5, the method for making of microbial fertilizer such as embodiment 1.
Microbial fertilizer is as follows in fermentation fowl and animal excrement method:
Inoculum size with 0.5% is sprayed onto on fowl and animal excrement and the stalk mixture, and fermentation can obtain biological organic fertilizer more than 20 days.
Microbial fertilizer of the present invention has joint fertilizer, volume increase, improves non-specific immunity enhancing resistance, alleviates the effect of soil compaction enhancing land fertility.
Embodiment 6, the method for making embodiment 1 of microbial fertilizer of the present invention.
Usage and the effect of microbial fertilizer of the present invention on potato is as follows:
1, seed soaking: seed can be put into container, then microbial fertilizer of the present invention be dressed seed to water, every mu of consumption 500ml.
2, spray: can convert 200 times of water dilutions, foliage-spray, every mu of consumption 1000ml.
Embodiment 7, and the usage and dosage of microbial fertilizer of the present invention on soybean is as follows:
1, seed dressing: seed can be put into container, then the multi-joint microbiobacterial agent that closes vinelandii be dressed seed to water,, every mu of consumption 500ml.
2, spray: can convert 200 times of water dilutions, foliage-spray, every mu of consumption 1000ml
3, fermentation fowl and animal excrement: the inoculum size with 0.5% is sprayed onto on fowl and animal excrement and the stalk mixture, and fermentation can obtain biological organic fertilizer more than 20 days.
Claims (7)
1. microbial fertilizer, it is characterized in that: described microbial fertilizer comprises following components by weight ratio:
Bacillus cereus culture 10～30%, Bacillus subtillis culture 10～30%, pantoea agglomerans culture 10～30%, plant lactobacillus culture 10～30% and lactobacillus acidophilus cultures 10～30%.
2. microbial fertilizer as claimed in claim 1 is characterized in that: described microbial fertilizer comprises following components by weight ratio:
Bacillus cereus culture 20%, Bacillus subtillis culture 20%, pantoea agglomerans culture 20%, plant lactobacillus culture 20% and lactobacillus acidophilus cultures 20%.
3. microbial fertilizer as claimed in claim 1 is characterized in that: described Bacillus cereus culture is single Bacillus cereus strain culture.
4. microbial fertilizer as claimed in claim 1 is characterized in that: described Bacillus cereus culture is the mixture of two or more Bacillus cereus strain cultures.
5. microbial fertilizer as claimed in claim 4 is characterized in that: described Bacillus cereus culture is the mixture of Bacillus cereus BS8641 and Bacillus cereus AS2015 strain culture.
6. microbial fertilizer as claimed in claim 5 is characterized in that: the weight ratio of described Bacillus cereus BS8641 and Bacillus cereus AS2015 strain culture is 1: 1.
7. as the preparation method of claim 1-6 a kind of microbial fertilizer as described in one of them, it is characterized in that: described preparation method comprises the steps:
A, preparation Bacillus cereus and Bacillus subtillis fermented liquid: Bacillus cereus and Bacillus subtillis bacterial strain seed liquor are inserted in the culture medium after sterilization in 1: 1 ratio, substratum comprises that weight ratio is the bean sprouts juice of 5-10%, the glucose of 0.2-0.5%, the peptone of 0.5-1.5%, surplus is the water as solvent, inoculum size is 0.3%, be under the condition of 7.2-8.5 aerobic fermentation 16-28 hour at 32 ℃-36 ℃, PH, put jar, make Bacillus cereus and Bacillus subtillis fermented liquid;
B, the agglomerating general fermented liquid of preparation: the pantoea agglomerans seed liquor is inserted in the culture medium after sterilization, substratum comprises that weight ratio is the soyflour of 1%-3%, the Semen Maydis powder of 1%-2%, the yeast extract paste of 0.1-0.5%, the white sugar of 1-3%, the monosodium glutamate of 0.1-0.3%, the potassium primary phosphate of 0.1-0.3%, the lime carbonate of 1-3% and the sodium-chlor of 0.3-0.6%, surplus is the water as solvent, inoculum size is 0.3%, at 30-35 ℃, PH is under the 7.2-8.5 aerated culture 36-48 hour, put jar, obtain the pantoea agglomerans fermented liquid;
C, preparation Lactobacterium acidophilum and plant lactobacillus fermented liquid: Lactobacterium acidophilum and lactobacterium plantarum strain seed liquor liquid are inserted in the culture medium after sterilization in 1: 1 ratio, substratum comprises that weight ratio is the glucose of 1%-3%, the peptone of 0.5-1.5% and the yeast extract of 0.5-1%, surplus is the water as solvent, inoculum size is 0.3%, behind 32 ℃ of-36 ℃ of following anaerobically fermentings, obtain Lactobacterium acidophilum and plant lactobacillus mixed fermentation liquid fermented liquid.
D, the culture fermentation broth that obtains is made microbial fertilizer according to above-mentioned weight proportion.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102277343A (en) * | 2011-07-24 | 2011-12-14 | 李绩 | Biological composite enzyme |
| CN102488120A (en) * | 2011-12-28 | 2012-06-13 | 广西东园生态农业科技有限公司 | Preparation method of fermenting ensilaged feed |
| CN102951957A (en) * | 2011-08-29 | 2013-03-06 | 保罗生物园科技股份有限公司 | Microbial viable bacterium fruit tree water flush fertilizer, preparation technology and application method thereof |
| CN104694422A (en) * | 2015-02-11 | 2015-06-10 | 齐齐哈尔大学 | Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons |
| CN106190914A (en) * | 2016-07-22 | 2016-12-07 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of compound lactobacillus microbial inoculum promoting Fructus Fragariae Ananssae to grow and application thereof |
| CN106544337A (en) * | 2016-11-28 | 2017-03-29 | 北京光禾生物科技有限公司 | A kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier and preparation method thereof |
| CN106831265A (en) * | 2017-03-28 | 2017-06-13 | 安徽中煦环保新材料科技有限公司 | A kind of soil ecology repair materials and preparation method thereof |
| CN113575249A (en) * | 2021-07-14 | 2021-11-02 | 运城学院 | Seedling growing method for improving medicinal value of forsythia fruits |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102277343A (en) * | 2011-07-24 | 2011-12-14 | 李绩 | Biological composite enzyme |
| CN102951957A (en) * | 2011-08-29 | 2013-03-06 | 保罗生物园科技股份有限公司 | Microbial viable bacterium fruit tree water flush fertilizer, preparation technology and application method thereof |
| CN102488120A (en) * | 2011-12-28 | 2012-06-13 | 广西东园生态农业科技有限公司 | Preparation method of fermenting ensilaged feed |
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| CN104694422A (en) * | 2015-02-11 | 2015-06-10 | 齐齐哈尔大学 | Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons |
| CN106190914A (en) * | 2016-07-22 | 2016-12-07 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of compound lactobacillus microbial inoculum promoting Fructus Fragariae Ananssae to grow and application thereof |
| CN106190914B (en) * | 2016-07-22 | 2019-05-14 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | It is a kind of promote strawberry growth compound lactobacillus microbial inoculum and its application |
| CN106544337A (en) * | 2016-11-28 | 2017-03-29 | 北京光禾生物科技有限公司 | A kind of microbial bacterial agent containing Herba Menthae powder with activated carbon as carrier and preparation method thereof |
| CN106831265A (en) * | 2017-03-28 | 2017-06-13 | 安徽中煦环保新材料科技有限公司 | A kind of soil ecology repair materials and preparation method thereof |
| CN113575249A (en) * | 2021-07-14 | 2021-11-02 | 运城学院 | Seedling growing method for improving medicinal value of forsythia fruits |
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Application publication date: 20110706 |