CN103725634A - Compound probiotics for planting kiwi fruits - Google Patents
Compound probiotics for planting kiwi fruits Download PDFInfo
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- CN103725634A CN103725634A CN201310747376.4A CN201310747376A CN103725634A CN 103725634 A CN103725634 A CN 103725634A CN 201310747376 A CN201310747376 A CN 201310747376A CN 103725634 A CN103725634 A CN 103725634A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to compound probiotics for planting kiwi fruits. The compound probiotics can be applied to soil remediation, bacterial manure fabrication, irrigation, foliar application, spraying on the ground, air atomizing sterilization and the like. Thus, the yield, the quality and the disease resistance of the kiwi fruits are improved.
Description
Technical field
The invention belongs to microbial technology field, more specifically, relate to a kind of compound probiotic for Kiwifruit plantation.
Background technology
The growing environment of Kiwifruit, the distribution of root system in hard soil layer is more shallow, and the darker characteristic that can distribute in loose soil can well-grown on acidity, subacidity (P in soil H 5.5-6.5) or neutral soil.The Kiwifruit of existing plurality area plantation, because of improper chemical fertilizer, agricultural chemicals and the swelling agent used year after year, often causes fruit quality poor, yielding ability is not strong, output is lower, and disease resistance performance is poor, and for example bacterial canker and fungal leaf spot have a strong impact on the yield and quality of Kiwifruit.
Compound probiotic is applied in Kiwifruit planting process, can better suppress the generation of disease, improves Kiwifruit yield and quality.
Summary of the invention
One. the effect of compound probiotic in plantation
1. the part of plant on earth's surface is cauline leaf, and it is carrying out photosynthesis, carrying out energy transformation.
A. using the compound probiotic that contains milk-acid bacteria as foliage fertilizer, be sprayed on plant leaf and limb, milk-acid bacteria can increase the effect of light compositing bacterium, and light compositing bacterium can promote the photosynthesis of plant, the formation of fortification of plants body to vitamin C and E, therefore, by being sprayed at plant leaf and limb above take milk-acid bacteria as main compound probiotic, can promote the photosynthesis of plant, produce a large amount of chlorophyll, guaranteed luxuriance of vegetation and health.
B. as pathogenic bacteria and viral natural enemy, the safest sterilizing of milk-acid bacteria or diseases and pests of agronomic crop and sterilant.After the compound probiotic that contains milk-acid bacteria is diluted according to a certain percentage, be sprayed at plant leaf and limb upper, sprayed the cauline leaf surface journey subacidity after milk-acid bacteria, order about insect escape, also the edible food of insect that just broken, insect cannot, in the existence of cauline leaf environment, reach the effect of desinsection expelling parasite; Thereby realize, kill some pathogenic microorganisms and kill some insect, as agrotis larva (formal name used at school: prodenia litura), native acarid (tick), various leaf tick worm, larva take great Ye grass as food etc.Avoid a large amount of use chemical insecticides, guaranteed food safety.
2. the part of plant under earth's surface is mainly root system, and it makes plant from soil, draw various desired nutritionals, carrying out matter and energy conversion.
A. by by spoilage organism etc. cause corrupt soil, germicidal treatment before planting; A certain proportion of compound probiotic that contains milk-acid bacteria is sprayed, turned over, thereby suppress the growth of spoilage organism, make it without living space, prevent root rot.This is milk-acid bacteria " soil disinfectant " function.
B. utilize take milk-acid bacteria as main compound probiotic and carry out compost treatment, make microbial organic fertilizer, the soil before plantation is carried out to microbial organic fertilizer and topdress, can improve crop yield by fertilising, improve product quality, maintain and increase soil fertility.Increase soil nutrient, avoid the too much chemical fertilizer of using.
C. the compound probiotic that contains milk-acid bacteria by a certain percentage adds water and irrigates, and improves soil microorganisms environment and root exudates environment, makes its equilibrium, makes plant root more easily absorb nutrient; A point stem phase to plant plays a part very important.
D. because milk-acid bacteria is sugary and lactic acid, and earthworm has a sharp sense of taste, happiness sweet food and tart flavour, therefore can amount reproduction as the earthworm in soil, and earthworm can make loosing soil, make moisture and fertilizer be easy to enter and improve the fertility of soil, be conducive to the growth of plant.
E. soil heat preservation function: due to milk-acid bacteria anaerobism breeding under soil table, driven a series of beneficial microorganism activities, these microorganism growth metabolism are that the soil moisture improves 1-2 ℃.In the north, can play the function of protecting plant root.
F. soil water-retaining function: because milk-acid bacteria is microbial bacterial, among its cells in vivo, moisture is approximately 70%, while having a large amount of milk-acid bacteria, can keep soil moisture.Aerobe is survived at upper soll layer, and anaerobies bacterium survives under earth's surface, and spoilage organism cannot be survived under earth's surface, guarantees that plant seed issues blastogenesis root, growth at the environment of safety, forms gradually ground flora, and then reaches water and soil and do not run off.
G. trophism: take milk-acid bacteria as main compound probiotic is in growth metabolism process, produce multivitamin, organonitrogen, soluble phosphate, amino acid etc., when plant root nutrition can be provided, can also obviously increase the content of food protein, sugar, VITAMIN and aromatoising substance, increase the dry-matter proportion of fresh and tender food, thereby Food Quality, local flavor, keeping quality are improved, color and luster, outward appearance etc. are obviously improved, and this special role is that chemical fertilizer is irreplaceable.
H. after the compound probiotic that contains milk-acid bacteria being combined with other fibrous substances, join in soil, dig under earth's surface, carry out spontaneous fermentation, can reduce gradually nitrate ion concentration, solve acidification problem.
Under the effect of the compound probiotic that contains milk-acid bacteria, after optimumization of plant rhizosphere environment, plant stem-leaf is also just grown up healthy and sound, and insect cannot be invaded food from root; To depending on the aquaculture of plant, be also very useful, plant is as the beginning of cultivation chain, to the prevention of aquaculture pathogenic bacteria and virus, do not need to re-use antibiotic etc, guaranteed the pure natural property of plant, meanwhile, also reduce the residual harm bringing to people's health of environmental pollution and agricultural chemicals, thus the world that can realize nature probiotic bacterium and human life's symbiosis.
Two, the compound probiotic of different purposes forms
1. " compound probiotic " that soil remediation is used: containing milk-acid bacteria, yeast, actinomycetes bacterium, photosynthetic bacterium, genus bacillus etc.
2. make " compound probiotic " that microbial-bacterial fertilizer is used: containing compound probiotics such as milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus.
3. " compound probiotic " that pouring is used: containing compound probiotics such as milk-acid bacteria, yeast, nitrobacteria, genus bacillus.
4. cauline leaf sprays use " compound probiotic ": containing compound probiotics such as photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus.
5. ground sprays use " compound probiotic ": containing compound probiotics such as milk-acid bacteria, yeast, genus bacillus.
6. " compound probiotic " that air atomizing degerming is used: containing compound probiotics such as milk-acid bacteria, yeast, genus bacillus.
Three. microbial species planting technology is as follows at the concrete function of Kiwifruit plantation:
1. increase soil fertility, chessom, maintenance soil moisture, form good rhizospheric environment, promote, outside root absorption nitrogen phosphorus potassium, to absorb iron, zinc, magnesium, manganese etc. simultaneously, impel plant leaf more healthy carry out photosynthesis.
2. increase fruit resistance, can effectively kill spoilage organism in soil, prevent and treat root rot and fruit tree diseases and pests; As the Peptic Ulcers of Kiwifruit, blossom rot, apple olethreutid, the control of root knot nematode etc.
3. promote photosynthesis, make the more flourishing of fruit growth
4. improve fruit quality, prolong storage period
5. increasing both production and income.
Embodiment
The obtain manner of compound probiotic.
Milk-acid bacteria, yeast, subtilis, photosynthetic bacterium, actinomycetes, nitrobacteria bacterial classification are all purchased from market.
Produce the fermented liquid of milk-acid bacteria, yeast, subtilis, photosynthetic bacterium, actinomycetes, nitrobacteria, then above-mentioned fermented liquid is composited in proportion first respectively.
The production method of milk-acid bacteria is:
In 1kg water, add brown sugar 0.05～1.0kg, be configured to after solution, add 5g～10g lactic acid bacteria culturers, sealed fermenting in anaerobic fermentation tank, 33～38 ℃ of controlled fermentation temperature, fermentation time 3～7 days, the liquid spawn having activated is added to second order fermentation tank sealed fermenting, second order fermentation liquid proportioning is: molasses 10%, amino acid 0.1%～0.5%, 33～38 ℃ of VITAMIN 0.05% ~ 1% controlled fermentation temperature, fermentation time 3～7 days, inoculum size 5%～10%, between yeast phase, constantly add molasses, treat that bacterial classification concentration reaches 300,000,000/ml, pH is 4.2 o'clock, stop fermentation.
Saccharomycetic production method is:
By being inoculated into bacterial classification in yeast, expand in fermented liquid, the formula of fermented liquid is: glucose 50g/L, urea (NH
4)
2sO
41g/L, KH
2pO
42.5g/L Na
2hPO
40.5g/L, MgSO
41g/L, FeSO
40.1g/L, yeast extract paste 0.5g/L, rose-bengal 0.03g/L, pH4.5-5.0, sealed fermenting in aerobic fermentation tank, controlled fermentation temperature 25-28 ℃, fermentation time 2-3 days, the liquid spawn having activated is added to the fermentation of secondary aerobic fermentation tank, second order fermentation liquid proportioning is: soybean sprout 100g/L, glucose 50g/L, pH nature, controlled fermentation temperature 25-28 ℃, fermentation time 3～5 days, inoculum size 5%～10%, constantly adds glucose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation.
The production method of subtilis is: subtilis is seeded in substratum, culture medium prescription is: peptone 1 %, yeast extract 0. 5 %, sodium-chlor 1 %, nature pH, is placed in 37 ℃, seed fermentation tank and cultivates after 1-2 days, access fermention medium, culture medium prescription is: sucrose 1 %, peptone 1 %, Sodium phosphate dibasic 0. 2 %, SODIUM PHOSPHATE, MONOBASIC 0. 2 %, in natural pH, inoculum size 5%～10%, 35～37 ℃ of temperature, constantly add sucrose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation.
The production method that it is characterized in that described photosynthetic bacterium is: photosynthetic bacterium is seeded in yeast extract paste protein culture medium, and basic recipe is: CaCL
20.3g, MgSO
47H
2o 0.5g, yeast extract paste 3g, peptone 3g, distilled water 1000ml, adjusts pH 6.8, in culture temperature: 25 ℃～30 ℃, intensity of illumination: 2000LX～5000LX, lower cultivation 3 days, afterwards in aerobic fermentation tank with 15% inoculum size, 25 ℃～30 ℃ of temperature, intensity of illumination: 2000LX～5000LX, cultivates under the condition of pH:7.5～8.5, and in fermenting process, results of regular determination pH also carrys out neutralise broth with 150g/L Sulfothiorine, until bacterial classification concentration, reach 300,000,000/ml when above, stop fermentation.
Actinomycetic production method is: bacterial classification is inoculated in No. 1 substratum of Gao Shi (GA), and main component is: Zulkovsky starch 20g, KNO
31g, K
2hPO
40.5g, MgSO
47H
2o 0.5g, NaCl 0.5g, FeSO
47H
2o 0.01g, pH=7.4-7.6, water 1000ml enlarged culturing is controlled 28～30 ℃ of temperature in same medium with 5%～10% inoculum size afterwards in aerobic fermentation tank, and pH7.0-7.6 fermentation culture reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation.
The production method of nitrobacteria is: nitrobacteria is seeded in substratum, and culture medium prescription is: ammonium sulfate 0.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, SODIUM PHOSPHATE, MONOBASIC 1g, magnesium sulfate 0.03g, CaCl
27.5g, distilled water 1000ml, in pH 7.5～8.0, enlarged culturing 3 days under 28～30 ℃ of conditions of temperature, with 5%～10% inoculum size, in the aerobic large fermentation tank of same medium, control 28～30 ℃ of temperature afterwards, pH7.5-8.0 fermentation culture, reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation.
The composition of compound probiotic:
1. " compound probiotic " that soil remediation is used: milk-acid bacteria, yeast, genus bacillus, photosynthetic bacterium, actinomycetes by weight compositely proportional are: (5～8): (1～3): (0～1): (1～2): (0～1), preferably (5～6): (1～2): (0～1): (1～2): (0～1), most preferred ratio is 5:1:1:2:1.
2. make " compound probiotic " that microbial-bacterial fertilizer is used: milk-acid bacteria, yeast, genus bacillus, actinomycetes compositely proportional is by weight: (3～5): (1～2): (3～5): (1～2), preferably (3～4): (1～2): (4～5): (1～2), most preferred ratio is 3:1:4:1.
3. " compound probiotic " that pouring is used: milk-acid bacteria, yeast, genus bacillus, nitrobacteria by weight compositely proportional are: (5～8): (1～3): (1～2): 1, preferably (6～7): (1～2): (1～2): 1, most preferred ratio is: 6:2:1:1.
4. cauline leaf sprays use " compound probiotic ": milk-acid bacteria, yeast, photosynthetic bacterium, genus bacillus by weight compositely proportional are: (5～8): (1～3): (0～1): (1～2), preferably (5～6): (1～2): 1:(1～2), most preferred ratio is 5:2:1:2.
5. ground sprays use " compound probiotic ": milk-acid bacteria, yeast, genus bacillus by weight compositely proportional are: (5～8): (1～3): (1～2), what more have choosing is (6～8): (1～2): 1, and most preferred ratio is 8:1:1.
6. " compound probiotic " that air atomizing degerming is used: milk-acid bacteria, yeast, genus bacillus by weight compositely proportional are: (5～7): (2～3): (1～2), what more have choosing is (6～7): (2～3): 1, and most preferred ratio is: 6:3:1
The compound probiotic that contains milk-acid bacteria is taken as the leading factor with Lactobacterium acidophilum, and the carbohydrate that it produces by picked-up photosynthetic bacteria, yeast forms lactic acid, and milk-acid bacteria has very strong sterilizing ability, can effectively suppress the movable and organic sharply corruption of harmful microbe and decompose.It can decompose not labile xylogen and Mierocrystalline cellulose under normal conditions, and organic matter fermentation is decomposed.
It also has an effect can suppress exactly the pathogenic propagation of continuous cropping obstacle, and harmful nematode will sharply reduce, and crop just recovers rapidly growth.Can by following step in Kiwifruit plantation with compound probiotic, suppress the generation of root knot nematode.
According to the present planting soil corruption in planting fruit trees garden, cause the situation of various diseases, administer in accordance with the following steps:
If the root rot that 1. exists spoilage organism (the various spoilage organism in soil) to cause in soil, needs with milk-acid bacteria sterilizing and in conjunction with irrigation method, existing soil is carried out to sterilising treatment (implementation method refers to embodiment mono-).If there is not the situation of soil corruption in soil, according to method described below, plant.
2. after to soil treatment, in plantation, use the organic farm manure of microorganism, soil is carried out to organic improving, increase content and the fertility of probiotic bacterium in soil, form good rhizospheric environment and abundant trophoplasm, increase plant root health, improve Root Absorption ability, improve via plant immunity, prevent root rot (implementation method refers to embodiment bis-).
3. microorganism is added irrigation, is to supplement root probiotic bacterium and trophoplasm, adds compound probiotic liquid (implementation method refers to embodiment tri-) when irrigating.
4. foliage-spray, increases the synthetic of plant chlorophyll, urges and makes blade face light compositing, improves disease-resistant, insect-prevention ability, and improves the trophoplasm (implementation method refers to embodiment tetra-) of fruit.
5. ground and trunk expelling parasite, prevents external worm, the erosion of bacterium to fruit tree from ground, and fruit tree trunk and trunk around ground spray (implementation method refers to embodiment five).
plantation Application Example
Embodiment 1. soil sterilizings are administered
The corrupt improvement method in plantation soil
If soil does not have corruption and causes root rot, do not need to carry out this operation.Also can administer by the following method the soil before lower Kiwifruit sapling, can break off fruit tree seedlings from root like this and carry harmful bacteria.
1. soil treatment is used and is closed probiotic bacterium stoste containing milk-acid bacteria, yeast, actinomycetes bacterium, photosynthetic bacterium, genus bacillus etc.: every 100 square meters are used after 20 times of 5kg compound probiotic stoste dilute with waters, and available atomizer is evenly sprayed at will be administered on soil.
2. compound probiotic is evenly sprayed at and will be administered behind soil, turn over one time with rotary cultivator, xylophyta is ploughed deeply 30cm(to wanting the tree of result, the degree of depth is not to hurt shallow root place, can't injure root), and then spray once-combined probiotic bacterium, laterally turn over once with rotary cultivator, then spray hug after compound probiotic flat.Unwanted bacteria major part after for some time in soil is killed, and recovers gradually the balance of nature of soil.
This method with the following Examples 3 method use subsequently, also have good effect.
embodiment 2. compost are produced
Embodiment 2.1 microbial organic fertilizer making methods
1. compost is used containing compound probiotic stostes such as milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus.Usage ratio is 5% to be the compound probiotic stoste that compost per ton is used 50kg to contain milk-acid bacteria, spreads by atomizers spray, and compost material high humidity I is with suitable thin up probiotic bacterium stoste according to circumstances, to guarantee the normal fermentation of compost.
2. adopt artificial or mechanical mixing type to add bacterium stirring means: to turn over the loam that spreads thin layer, and then evenly sprinkle compound probiotic and stir, turn over subsequently broadcast application native while spread bacterium.Turn over and shed after bacterium finishes, compost bottom margin, hand mixing again, spills to dunghill middle and upper part being uniformly distributed in the hope of probiotic bacterium in dunghill.Then broken muck bat block dunghill surface, cover one deck plastic cloth and carry out fermentation maturity.
If conditions permit can be carried out compost by following formula: 1 cube of 1 cube of chicken manure, cow dung (also can be used fallen leaves stalk to replace or add stalk, but must be ground into 5-10mm left and right size), compound probiotic 5%, sugared slag 5%, ground phosphate rock 3%, peat composed of rotten mosses powder 3%, also can add the trace element of little amount 0.5-1%, as magnesia (magnesium oxide), iron ore powder and manganese ore powder etc., the about 850kg of finished product fertilizer making, humidity is pinched the degree that can just roll into a ball with hand, approximately 50～65% left and right.Use stirrer can make to spray milk-acid bacteria and add other attached material more even, be more conducive to fertilizer fermentation, shorten fermentation time etc.
3. the weather of temperature more than 20 ℃, more than covering film anaerobic fermentation temperature rise to 55 ~ 60 ℃, maintains 2 ~ 3 days and can open afterwards fertile heap, carries out secondary compound probiotic and sprays compost treatment.Turn over one deck loam and spray a probiotic bacterium, concrete consumption is consumption 5-10kg per ton, then air storage.After 2 ~ 3 days, carry out again a turning, mend bacterium, after air storage for some time, can use (as conditions permit, can turning in every 3 days mend bacterium once, be conducive to becoming thoroughly decomposed of fertilizer).
4. at the beginning of compost is preferably in annual 4 months, start, weather temperature raises, and processes and can do base fertilizer use in 1-2 month.
If 5. use the finished product fertilizer of buying as base fertilizer, the compound probiotic that can be directly adds 2kg to contain milk-acid bacteria etc. according to every 100kg fertilizer, adds water 5kg to stir, and applies to plant in soil and makees base fertilizer.
6. in order to guarantee the effect of compost, not mixed with the series products of other starters while using this product.
Embodiment 2.2, actinidia tree fallen leaves carry out compost utilization
Compound probiotic+stalk (fallen leaves) compost
A. use pulverizer that stalk (fallen leaves) is ground into 5-10mm large;
B. dig in the ground one dark 1 meter, the heatable adobe sleeping platform that the long X of certain size is wide, length and width are determined according to the amount of stalk (fallen leaves), not too big (about 5-15 ㎡) in principle, no person is not high to utilizing in soil bacterium effect; The immature soil digging out, gives over to stand-by;
C. stalk (fallen leaves) is put into the end, hole, during 5 centimeters of thickness, spray containing compound probiotic stoste 4-5L/ ㎡ such as milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus, then spray raw excrement 5kg/ ㎡ (people's animal dung all can), during without raw excrement, also can use, repave the immature soil (do not have large bulk in the immature soil, be loose shape as far as possible) of 3 centimeters of left and right; And then put into successively stalk (fallen leaves) → compound probiotic → life excrement → immature soil, until during 10 centimeters, tool earth's surface, compacting as far as possible, by Bas Discharged;
For observing leavening temperature, can be at centre-height place an embedding electronic thermometer, display screen extends on limit, hole, ground, to observe;
D. use plastics film to cover surface, surrounding compacting, places air and enters from surrounding;
E. on burying on film, the immature soil is to floor level again;
F. in fermenting process, observe temperature, if core temperature reaches 40-55 degree or higher, and can continue this temperature 1-2 days or more than, prove that fermenting process make good progress.Fermentation 30-45 days, according to different time length difference in season;
G. the fertilizer fermenting is taken out and used, or pack is preserved; Preferably according to 1% of amount of organic fertilizer, spray compound probiotic before use again, after stirring, re-use, effect can be better.
Embodiment 2.3 compound probiotics+chicken manure organic compost
Do not use the chicken manure of compound probiotic cultivation to carry out organic compost:
A. measure the water content of chicken manure and the ammonia amount of release, the live chickens excrement of caking is pulverized;
B. according to the water content of chicken manure and stink, according to the ratio of 5-10%, add compound probiotic, use stirrer fully to stir;
C. pack sealing, puts into plastic containers, and compacting is also extruded air, then container sealing is good as far as possible;
D. place 30-45 days with top fermentation, then take out and dry dehydration, pack to be used.
application process:according to the requirement of different fruit trees, execute at root or soil table and all can; With root, extend under certain distance and bury better, what lower buried depth degree depended on fruit tree prolongs the root degree of depth.
Spraying fertilizer (take red kiwi fruit as example)
One. bearing tree fertilising
1, ground fertilising
(1) fertilization time: conventionally annual pluck after fruit bring into use, to next year to pluck fruit after date be a circulation: pluck after fruit to entering before winter fallen leaves, use 1～2 time, according to the situation of bearing fruit of last year and trees growth situation, determine 1 time or 2 times;
After fallen leaves, before entering the winter, execute again 1 time;
Must use disposable fertilizer flower early stage (before blooming the beginning of spring);
Bear fruit the phase (Xie Huahou) use again once, red kiwi fruit imposes thanking after spending for 15-20 days.
Can bring into use from flower early stage (blooming the beginning of spring), but effect may not reach the effect of a whole circulation yet.
(2) fertilization effect: impel Fruit, the strong tip, and root system is grown and summer shoot growth and bud differentiation, core are painted has a good effect for the second time, can not only improve standard fruit rate then, can also lay the foundation for carrying out the long tree of annual bearing, obtain stable high yield year after year.
(3) amount of application: amount of application will be according to the age of tree, tree vigo(u)r, result quantities and determined;
(4) fertilizing method:
The one, annular groove is excuted a law, exactly on both sides, tip of a root areal concentration or four limits dig the ditch of dark 15cm, wide 20cm, long 100cm left and right.
The 2nd, spread manuer in holes, in tip of a root areal concentration surrounding, dig the cave of 10 left and right, the dark 15cm in cave, cave 3～5 cm.
Two. treelet fertilising
1, plant then seedling fertilization
In 5～June, monthly execute once.
2, raw tree fertilising in 1～2 year
Executing outward May from trunk 50cm.
Digging dark 15cm, wide 20 cm ring-type ditch spreads, the earth backing again of pouring water after fertilising June beyond trunk 50cm.
3, raw treelet fertilising in 3～5 years
3～5 years raw trees, for firstfruits tree, are executed ditch depth 15cm, wide 20cm, the earth backing again of pouring water after fertilising in tip of a root areal concentration ring-type in May.
Embodiment 3. fills with radical cure reason
Fill with the general dilution compound probiotic that uses: 500～1000 times of compound probiotic stoste (containing milk-acid bacteria, yeast, nitrobacteria, genus bacillus etc.) dilute with waters, carry out according to the normal irrigation water yield.According to the acidproof situation of fruit variety, can be every inferior or repeatedly interpolation.
Embodiment 4. foliage-sprays
Compound probiotic foliage-spray method
1. foliage-spray: the compound probiotic (containing compound probiotics such as photosynthetic bacteriums, milk-acid bacteria, yeast, genus bacillus) that just can carry out cauline leaf after fruit tree leafing and spray dilution, milk-acid bacteria in probiotic bacterium can promote the effect of light compositing bacterium, and light compositing bacterium can directly promote the photosynthesis of plant.The compound probiotic of dilution is in proportion sprayed on blade, produces a large amount of chlorophyll, guarantee luxuriance of vegetation and health.It is the absorption that plant stem-leaf has additional nutrients that the metabolic activity of compound probiotic can provide amino acid and various VITAMIN, Promoting plant growth, and can play the effect (full-bloom stage can not spray) of sterilization, and after result, still can spray, according to circumstances can within every 10 days, spray once.
2. cauline leaf sprays and uses dilution compound probiotic: by 500～1000 times of compound probiotic dilute with waters, every mu of ground is used about 200L compound probiotic diluent, is on the vaporific cauline leaf that is evenly sprayed at plant and on limb.
If atomizer sprayed in the past chemical sterilization sterilant and so on, re-use after do cleaning up.
5-June of red kiwi fruit is on the occasion of Fruit, and spring and summer tip leaf growth animated period is both large to various fertilizer element requirements, is also easily absorbed, and this phase is the most obvious from blade face Additional nutrient solution fertilizer efficiency fruit.
Embodiment 5. ground and trunk deinsectization expelling parasite processing
Compound probiotic ground spraying method
1. use the compound probiotic stoste containing milk-acid bacteria, yeast, genus bacillus etc., in the diameter range of 1～2 meter main trunk around, with the consumption of 50～100 milliliters, every tree, spray, can be according to disease and insect amount increasing usage quantity.
2. main trunk sprays, from ground along trunk upwards in 1 meter of altitude range.
Start weekly spray once, gradually reduce, until situation alleviate or disappear after stop.
Precaution: compound probiotic is living microorganism, can't mixes use with other chemical fertilizer, agricultural chemicals.
experiment effect comparison
By buying conventional compound probiotic microbial inoculum in contrast on market, commercially available prod 1 is the probiotic bacterium that Beijing company sells, and contains take photosynthetic bacteria, milk-acid bacteria, yeast as main probiotic bacterium and meta-bolites; The compound probiotic take milk-acid bacteria as master that sell for bio tech ltd, Yichuan commercially available prod 2.
The kind position Xu Xiang Kiwifruit of Kiwifruit for experiment.
Compound probiotic (experimental group 1), the commercially available prod 1(experimental group 2 of under equal experiment condition, with our company, producing), commercially available prod 2(experimental group 3) and do not use compound probiotic (control group) to plant processing to Kiwifruit, in the on all four situation of Cultivate administration mode, kiwifruit fruit is detected, and result is as follows:
1, Kiwifruit output experimental group 1 improves 15.1% compared with control group, compared with experimental group 2, improves 7.5%, compared with experimental group 3, improves 11.6%.
2, more homogeneous is full compared with control group outward appearance for fruit experimental group.
3, bacterium fungus disease experimental group is significantly improved compared with control group.
Experimental group mouthfeel is stronger mellow compared with control group, sour-sweet moderate, quality has clear improvement, amino acid, soluble solid, Vc, calcium, iron equal size are all significantly increased, experimental group 1 is also relatively strong compared with experimental group 2, experimental group 3 mouthfeels, quality is also improved, and soluble solid, Vc, calcium, the iron equal size detailed data that all increases sees the following form
Table 1 experimental group 1 and the contrast of control group Quality Detection
Table 2 experimental group 1 and experimental group 2 Quality Detection contrasts
Table 3 experimental group 1 and experimental group 3 Quality Detection contrasts
Claims (10)
1. for a compound probiotic for Kiwifruit plantation, mainly by milk-acid bacteria, formed.
2. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that soil remediation is used, containing milk-acid bacteria, yeast, actinomycetes bacterium, photosynthetic bacterium, genus bacillus etc.
3. probiotic bacterium claimed in claim 1, it is characterized in that can be used for making the compound probiotic that microbial-bacterial fertilizer is used: containing milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus etc.
4. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that pouring is used: containing milk-acid bacteria, yeast, nitrobacteria, genus bacillus etc.
5. probiotic bacterium claimed in claim 1, it is characterized in that can be used for cauline leaf and sprays the compound probiotic of use: containing photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus etc.
6. probiotic bacterium claimed in claim 1, it is characterized in that can be used for ground and sprays the compound probiotic of use: containing milk-acid bacteria, yeast, genus bacillus etc.
7. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that air atomizing degerming is used: containing milk-acid bacteria, yeast, genus bacillus etc.
8. the application of probiotic bacterium in Kiwifruit plantation described in claim 1-7, is characterized in that being produced, being filled with by soil sterilizing improvement, compost the modes such as radical cure reason, foliage-spray, ground deinsectization expelling parasite processing and air atomizing sterilizing insect-killing and applies.
9. the preparation method of probiotic bacterium described in claim 1-7, is characterized in that: produce first respectively milk-acid bacteria, yeast, subtilis, photosynthetic bacterium, actinomycetic fermented liquid, then above-mentioned fermented liquid is composited by weight.
10. the compound probiotic preparation method described in claims 9, is characterized in that:
The production method of described milk-acid bacteria is: in 1kg water, add brown sugar 0.05～1.0kg, be configured to after solution, inoculum size with 1%～5% adds lactic acid bacteria culturers, sealed fermenting in anaerobic fermentation tank, 33～38 ℃ of controlled fermentation temperature, fermentation time 3～7 days, the liquid spawn having activated is added to second order fermentation tank sealed fermenting, second order fermentation liquid proportioning is: molasses 10%, amino acid 0.1%～0.5%, 33～38 ℃ of VITAMIN 0.05% ~ 1% controlled fermentation temperature, fermentation time 3～7 days, inoculum size 5%～10%, between yeast phase, constantly add molasses, treat that bacterial classification concentration reaches 300,000,000/ml, pH is 4.2 o'clock, stop fermentation,
Described saccharomycetic production method is:
By being inoculated into bacterial classification in yeast, expand in fermented liquid, the formula of fermented liquid is: 50g/L, urea (NH
4)
2sO
41g/L, KH
2pO
42.5g/L Na
2hPO
40.5g/L, MgSO
41g/L, FeSO
40.1g/L, yeast extract paste 0.5g/L, rose-bengal 0.03g/L, pH4.5-5.0, sealed fermenting in aerobic fermentation tank, controlled fermentation temperature 25-28 ℃, fermentation time 2-3 days, the liquid spawn having activated is added to the fermentation of secondary aerobic fermentation tank, second order fermentation liquid proportioning is: soybean sprout 100g/L, glucose 50g/L, pH nature, controlled fermentation temperature 25-28 ℃, fermentation time 3～5 days, inoculum size 5%～10%, constantly adds glucose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation;
The production method of described subtilis is: subtilis is seeded in substratum, culture medium prescription is: peptone 1 %, yeast extract 0. 5 %, sodium-chlor 1 %, nature pH, is placed in 37 ℃, seed fermentation tank and cultivates after 1-2 days, access fermention medium, culture medium prescription is: sucrose 1 %, peptone 1 %, Sodium phosphate dibasic 0. 2 %, SODIUM PHOSPHATE, MONOBASIC 0. 2 %, in natural pH, inoculum size 5%～10%, 35～37 ℃ of temperature, constantly add sucrose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation;
The production method of described photosynthetic bacterium is: photosynthetic bacterium is seeded in yeast extract paste protein culture medium, and basic recipe is: CaCL
20.3g, MgSO
47H
2o 0.5g, yeast extract paste 3g, peptone 3g, distilled water 1000ml, adjusts pH 6.8, in culture temperature: 25 ℃～30 ℃, intensity of illumination: 2000LX～5000LX, lower cultivation 3 days, afterwards in aerobic fermentation tank with 15% inoculum size, 25 ℃～30 ℃ of temperature, intensity of illumination: 2000LX～5000LX, cultivates under the condition of pH:7.5～8.5, and in fermenting process, results of regular determination pH also carrys out neutralise broth with 150g/L Sulfothiorine, until bacterial classification concentration, reach 300,000,000/ml when above, stop fermentation;
Described actinomycetic production method is: bacterial classification is inoculated in No. 1 substratum of Gao Shi (GA), and main component is: Zulkovsky starch 20g, KNO
31g, K
2hPO
40.5g, MgSO
47H
2o 0.5g, NaCl 0.5g, FeSO
47H
2o 0.01g, pH=7.4-7.6, water 1000ml enlarged culturing is controlled 28～30 ℃ of temperature in same medium with 5%～10% inoculum size afterwards in aerobic fermentation tank, and pH7.0-7.6 fermentation culture reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation;
The production method of described nitrobacteria is: nitrobacteria is seeded in substratum, and culture medium prescription is: ammonium sulfate 0.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, SODIUM PHOSPHATE, MONOBASIC 1g, magnesium sulfate 0.03g, CaCl
27.5g, distilled water 1000ml, in pH 7.5～8.0, enlarged culturing 3 days under 28～30 ℃ of conditions of temperature, with 5%～10% inoculum size, in the aerobic large fermentation tank of same medium, control 28～30 ℃ of temperature afterwards, pH7.5-8.0 fermentation culture, reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation.
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| CN104782436A (en) * | 2015-03-30 | 2015-07-22 | 牟定县大丰农业开发有限责任公司 | Red-heart kiwi fruit canker control method |
| CN104904471A (en) * | 2015-06-01 | 2015-09-16 | 张亚南 | Strawberry planting method |
| CN104946550A (en) * | 2014-05-12 | 2015-09-30 | 山东黎昊源生物工程有限公司 | Leaf-protection biological strain repair agent |
| CN105541512A (en) * | 2015-12-04 | 2016-05-04 | 北京益生动力生物科技有限公司 | Ecological bacterial fertilizer and natural farming ecological planting method |
| CN105684622A (en) * | 2016-01-13 | 2016-06-22 | 广德原野水果种植家庭农场 | Efficient fruit tree fertilization method |
| CN106800490A (en) * | 2017-03-01 | 2017-06-06 | 苏州市相城区石新苗木产销专业合作社 | A kind of red kiwi fruit biological compound fertilizer for increasing fruit sweetness |
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| CN108164362A (en) * | 2018-01-11 | 2018-06-15 | 深圳森阳环保材料科技有限公司 | A kind of dedicated new bio-fertilizer of Kiwi berry |
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| CN110122335A (en) * | 2019-06-27 | 2019-08-16 | 西南林业大学 | The abductive approach of Xu Xiang Kiwifruit Tissue Culture seedling salt-tolerant mutant |
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Application publication date: 20140416 |