CN107667737A - A kind of method of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage - Google Patents

A kind of method of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage Download PDF

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CN107667737A
CN107667737A CN201710891792.XA CN201710891792A CN107667737A CN 107667737 A CN107667737 A CN 107667737A CN 201710891792 A CN201710891792 A CN 201710891792A CN 107667737 A CN107667737 A CN 107667737A
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vegetables
fruits
cfu
spore
soil
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CN107667737B (en
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唐志敏
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00—Methods for working soil
    • A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02—Superphosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C11/00—Other nitrogenous fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners

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  • Forests & Forestry (AREA)
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Abstract

The invention discloses a kind of method of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage, comprise the following steps：(1) before fruits and vegetables plant 7 10 days, using soil-repairing agent rehabilitating soil；(2) before fruits and vegetables seedling cultivation, soak fruits and vegetables root 0.05 0.08 hours using 1,500 2000 times of dilutions of immune vaccine or whole strain is soaked 0.01 0.05 hours；(3) in fruits and vegetables nursery stage, substrate soil is repaired using 300 400 times of dilutions of soil-repairing agent；(4) within the growth period of fruits and vegetables, applied fertilizer using biological organic fertilizer；(5) within the growth period of fruits and vegetables, the whole strain of fruits and vegetables is sprayed using 300 800 times of dilutions of immune vaccine.The method without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage of the present invention so that whole ecological prevention and control system pest and disease damage controllable rate is more than 90%；Absorption of the fruits and vegetables to nutriment can also be improved, strengthens the metabolic function of fruits and vegetables, improves photosynthesis, improves crop yield, lifts product quality；Whole process is green without applying pesticides.

Description

A kind of method of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage
Technical field
The invention belongs to field of agricultural cultivation, more particularly to a kind of side of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage Method.
Background technology
It is the topic constantly inquired into recent years to reduce agricultural chemicals plantation, but there is itself always in the course of cultivation for fruits and vegetables Healthy growth and pest and disease damage infect the problems such as endangering.Now in order to improve crop yield, people are in farming operations, raising crops In yield and the production process of preventing and treating pest and disease damage, the living needs in one period is ensure that by chemical farming, but it is long-term a large amount of Environmental pollution caused by chemical farming is used directly to jeopardize human health, referred to as global question of common concern.With The raising of human living standard, agricultural structure are adjusted to safe agricultural by devoting exclusive attention to output.In agricultural production, reduce or Abandon using chemical farming, development " organic agriculture " and " green agriculture " is the common aspiration and striving direction of the mankind.
Therefore, it is necessary to study a kind of method of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage, lifting fruits and vegetables itself are reached Resistance, improvement grows, and realizes the purpose of health and safety.
The content of the invention
The technical problems to be solved by the invention are to overcome the shortcomings of to mention in background above technology and defect, there is provided one Method of the kind without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage.
In order to solve the above technical problems, technical scheme proposed by the present invention is：
A kind of method of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage, comprises the following steps：
(1) before fruits and vegetables are planted 7-10 days, using soil-repairing agent rehabilitating soil；
(2) before fruits and vegetables seedling cultivation, fruits and vegetables root 0.05- is soaked using 1500-2000 times of dilution of immune vaccine 0.08 hour or whole strain immersion 0.01-0.05 hours；
(3) in fruits and vegetables nursery stage, substrate soil is repaired using 300-400 times of dilution of soil-repairing agent；
(4) within the growth period of fruits and vegetables, applied fertilizer using biological organic fertilizer；
(5) within the growth period of fruits and vegetables, the whole strain of fruits and vegetables is sprayed using 300-800 times of dilution of immune vaccine；
Wherein, the Contents of Main Components in the immune vaccine is：Biologically active polypeptide >=50g/L, chitosan oligosaccharide >=50g/ L, amino acid >=50g/L, sucrose water (sterilized water adds sucrose after being heated to 65-70 DEG C, is cooled to room temperature use) >=20g/L are multiple Close bacterium A >=50 × 109cfu/g；The compound bacteria A refers to beauveria bassiana spore >=50 × 109Cfu/g (units：Cfu/g, Mei Yike The living flora spore count contained), Trichoderma spore >=50 × 109Cfu/g, saccharomycete spore >=50 × 109Cfu/g, photosynthetic bacteria spore Son >=50 × 109Cfu/g, muscardine spore >=50 × 109Cfu/g compound bacteria.Wherein, enrichment culture used by compound bacteria A Base freezes 20%, starch 15%, sucrose (or glucose) 5%, distilled water (sterilized water) 60% by albumen and is formulated, and controls pH Between 6.5-7, compound bacteria A is inoculated into the enriched medium prepared, trained in sterile culture case in 27-29 DEG C of constant temperature Support 9-10 days, extract suspension bacteria liquid, low temperature storage is standby, using finishing (selection cloudy day, dusk or without sunlight direct projection in 8 hours Period uses, outdoor temperature wind scale≤1.5 grade).
Biologically active polypeptide in immune vaccine of the present invention can directly be absorbed by fruits and vegetables, promote the life of fruits and vegetables Long development.It during foliage-spray, can directly be absorbed by blade face cell, improve chlorophyll content, increased leaf area, strengthen photosynthetic work With promotion root cell grows, and improves root activity, promotes fruits and vegetables root system development, increases root system and soil contact, raising pair The absorption and use efficiency of nutrient and moisture, the immunocompetence enhancing that fruits and vegetables root system resists soil surface characters invasion is improved, so as to subtract The incidence of disease of few soil-borne disease.Chitosan oligosaccharide can suppress fruits and vegetables growth of pathogenic bacteria, effectively weaken the infection ability of pathogenic microorganism； Chitosan oligosaccharide is combined with the acceptor on fruits and vegetables cell, excites Signal molecules of resistance to produce such as NO, H2O2、Ca2+, jasmonic etc., pass through Signal transduction, excites tolerant gene expression, produces resisting substance, as fruits and vegetables antitoxin, chitinase, phenylalanine lyase, Polyphenol oxidase, peroxidase, catalase, superoxide dismutase, β -1.3 dextranases etc., so as to reach preventing and treating The purpose of disease.For chitosan oligosaccharide induction of auxin (IAA) synthesis, auxin signal, which is passed in fruits and vegetables body, plays growth regulating work With the activation a variety of gene expressions of growing of fruits and vegetables, synthesis is beneficial to the bioactive substance of fruits and vegetables growth, improves fruits and vegetables itself and resists Sick defence capability.There is good action to fruits and vegetables growth, output increased and quality improving.Amino acid is the precursor of synthetic protein Matter, using compound amino acid, fruits and vegetables plant physiologically active can be improved, increases chlorophyll content, improves the effect of photosynthetic rate；Can Strengthen peroxidase activity, reduce the content of MDA in plant, prevent the effect of cell membrane oxidation and cell ageing.Ammonia Base acid also has the effect for being passivated pathogen toxin.Amino acid and small peptide are used directly for synthesizing various enzymes in fruits and vegetables body, increase Strong fruits and vegetables anabolism ability, accelerates growing for fruits and vegetables, strengthens fruits and vegetables credit epidemic disease drag.Due to amino acid and organic matter and The addition of a large amount of profitable strains, there is provided the growth conditions of aerobic bacteria so that gas, solid, liquid, microorganism enter good in soil Property circulation, so as to play the effect for soil of fostering and apply fertilizer, improve the soil, support, good growing environment, expansion sterilization disease-resistant work are provided for compound bacteria A With.Sucrose can provide nutrient and original raw material for microorganism, increase the quantity of microorganism, so as to accelerate organic matter decomposition, make Plant food increase.Muscardine, high-efficiency broad spectrum, virulent biological insecticides, conidia of beauveria bassiana Cuticle, On stomata or alimentary canal, meet suitable condition and sprout, bear germ tube, while produce the dissolving elder brother such as lipase, protease, chitinase The epidermis of the insects such as worm, polypide is invaded by germ tube, the growth and breeding in polypide, the nutrient in pin main body is consumed, produces various Toxin, a large amount of mycelia and spore are formed, be covered with polypide whole body, reached the effect of preventing and treating fruits and vegetables insect pest, fruits and vegetables can be killed and had Insect pest.Trichoderma can suppress fruits and vegetables soil-borne pathogen and the partially biocontrol fungi of upper disease.In this method It is that screened trichoderma strain is done harm to for soil-borne venereal disease, is destroyed into parasitic and antibiosis mode and decompose pathogen bacterium Body, while fruits and vegetables root growth can be promoted, fruit-vegetable quality is improved, and the existing disease of fruits and vegetables can be killed.And saccharomycete is being cultivated During can produce substantial amounts of antibacterial substance, there is obvious bacteriostasis, saccharomycete and its fermentate can be improved 3 in fruits and vegetables body The generation of kind defense-related enzymes (POD, PAL and CAT)；Meanwhile the suppression to germs such as soft-rot fungis is also by producing hydrolysis Enzyme, dextranase and chitinase directly act on cause of disease mattress, and participate in inducing fruits and vegetables to produce resistance etc..By spraying in fruits and vegetables leaf It face, can rapidly be adhered on fruits and vegetables blade and fruit surface, form overcoat, improve and be immunized, resist disease.Photosynthetic bacteria liquid Blade face is sprayed after dilution can promote the increase of leaf Determination of Chlorophyll content, and the absorptivity and rate of photosynthisis for making carbon dioxide carry respectively Height, strengthen diphosphoribulose carboxylase (rubp) vigor；Also convertibly in earth microorganism species composition, it is bacterium, solid Nitrogen bacterium, Population of Actinomycetes increase, fungi amount reduce, so as to by improve fruits and vegetables itself photosynthetic efficiency and increase soil fertility come Play growth promotion, healthy and strong fruits and vegetables, improve the effect of resistance.Green muscardine fungus be can parasitize various pests a kind of desinsection it is true Bacterium, entered by body surface invasion effect in pest body, constantly increase breeding in pest body, by consuming nutrition, mechanical penetration, production Raw toxin, and constantly propagated in pest population, make insect lethal.And the present invention selection agricultural amino acid original powder be because：The One, amino acid inherently plant nutrient agent, amino acid is nutriment necessary to plant growth, sweet ammonia in agricultural amino acid Acid, lysine, glutamic acid, leucine have peculiar photosynthesis and adjustment effect to crops, and plant growth is particularly Photosynthesis has unique facilitation, and it can increase chlorophyll content of plant, improves the activity of enzyme, promotes carbon dioxide Infiltration, make photosynthesis more vigorous, to improve crop quality, increase Vc and sugar content suffer from important function；Second, Amino acid is effective plant growth regulator, and crop growth process needs various nutrients, material, these nutrition member Element, material absorb quantitative proportion and equilibrium condition in vivo, the nutrition physiology of crop influenceed it is very big, can direct relation To the quality of crop and fruit, and spray or fill to fruits and vegetables apply the material containing amino acid can increase in plant needed for various battalion Element is supported, aggravates the accumulation of dry and from plant root or leaf portion to the running speed at other positions and quantity, regulation is a large amount of The ratio and poised state of element, trace element and various nutritions, so as to play a part of adjusting plant normal growth； 3rd, amino acid is that the trace element such as a great number of elements such as strong complexing agent, N, P, K, Zn, Fe, Cu, Mn, B, Mo is to make Necessary material in object；The symptom for lacking some elements can often occur in crop, and the live part that can be absorbed by crops contains Measure very little, and chelatropic reaction can occur with slightly solubility element for amino acid, and protective effect is produced to element in formula, and generate dissolving The chelate spend, being easily absorbed by crops, so as to be advantageous to plant absorption, this amino acid also functions to the work of complexing agent in formula With.
The present invention passes through the raw material to immune vaccine and the control of content so that when spraying immune vaccine to fruits and vegetables, profit The telergone being immunized with fruits and vegetables, which drives, refuses insect or suppression disease microbial reproduction；When there is stress reaction in fruit and vegetable physiological regulation structure, Under fire position enriched antibody material or repairing material, suppression infection process, growth again, stimulate the raising of fruits and vegetables autoimmunity With the resistance to environmental pressure, fruits and vegetables fast-growth is stimulated and adjusted, promotes fruits and vegetables well developed root system；Fruits and vegetables are improved simultaneously to battalion The absorption of material is supported, strengthens the metabolic function of fruits and vegetables, improves photosynthesis, improves crop yield, lifts product quality.
Above-mentioned method, it is preferred that further comprising the steps of：Before the sowing of fruits and vegetables seed, using the 1000- of immune vaccine 1500 times of dilutions, soak fruits and vegetables seed 1-2 hours.
Above-mentioned method, it is preferred that the main component in the soil-repairing agent includes：Biologically active polypeptide >=100g/ L, agricultural amino acid original powder mass content >=2.0%, compound bacteria B >=100 × 109cfu/g；Wherein described compound bacteria B is to include The flora of following strain：Bacillus globigii spores 2 × 109Cfu/g, actinospore >=40 × 109Cfu/g, saccharomycete spore ≥40×109Cfu/g, nitrogen-fixing bacteria spore >=40 × 109Cfu/g, silicate bacteria spore >=40 × 109Cfu/g, Paecilomyces lilacinus Bacterium spore >=40 × 109Cfu/g ,/gram of compound bacteria spore >=500,000,000 of bacillusmusilaginosiengineering, photosynthetic bacteria spore >=5,000,000,000/ Gram, beauveria bassiana spore >=40 × 109Cfu/g, Trichoderma spore >=40 × 109Cfu/g, Bacillus cereus spores >=4 × 109Cfu/g, gluey subtilis spore >=4 × 109Cfu/g, aspergillus oryzae spore >=4 × 109Cfu/g, bacillus megaterium spore Son >=4 × 109cfu/g。
The soil-repairing agent selection bacillus subtilis of the present invention, bacillus subtilis energy metabolism in growth course are secreted Bacteriocin (subtilin, polymyxins, nystatin etc.), lipopeptide compound, organic acid substance etc.；These metabolins can Effectively suppress growth or the dissolving pathogen of opportunistic pathogen, so that germ is killed, high anti-continuous cropping；The enzyme that it secretes has chitin to cause enzyme Antibacterial protein has strong inhibitory action to a variety of fruits and vegetables original germs；And by the contention of space and nutritional ingredient, reach to causing The competitive inhibition of germ, Ant agonism, sterilize bacteriolysis；Bacillus subtilis can be on the mycelia of pathogen With growth, decomposition and consumption pathogen, pathogenic bacteria mycelia is broken, and preventing and treating pathogenic bacteria further infect plant；It can also use In the fruits and vegetables disease such as preventing and treating powdery mildew, rice blast, head blight, banded sclerotial blight, carbon myiasis, cucumber downy mildew, bacterial wilt of tomato, gray mold Evil.The vegetative hyphae major function of actinomyces is to absorb nutriment, can utilize different carbohydrate, including sugar, starch, Organic acid, cellulose, hemicellulose etc. are used as the carbon source energy, produce various enzyme preparations (protease, amylase and cellulase Deng), vitamin B12 and organic acid etc., impose in root soil, directly acted on pathogen, prevent and treat and withered caused by sickle-like bacteria Disease, suppress preventing and treating early blight germ and cucurbits powdery mildew, promote plant growth, actinomyces produce plant hormone by nitrogen fixation Make plant dry weight and chlorophyll content increase with growth promoter, stimulate root growth；Actinomyces colonize in pathogen dietetic bacterial In filament, make mycelium that modal distortion occur, so as to rupture, reach controlling disease effect.Line thalline, a variety of ferment can be produced Plain and natural antibiotics, can in soil amount reproduction, form dominant bacteria, except that can protect root circle, change rhizosphere microorganism Phase, and strengthen fruits and vegetables immune system.Saccharomycete can quickly change soil zwitterion structure, balanced soil acid-base value, increase soil Earth beneficial bacterium, activating soil, break hardened, dead soil change soil living, fertilizing soil, thoroughly exempt from deep ploughing, be not hardened；Anti- continuous cropping, subtract disease Evil, suppress various germs, anti-continuous cropping, the mitigation crop growth period diseases such as the fungi in soil, bacterium, chain knife bacterium and occur；Accelerate Various stalk corruptions become farm manure into organic matter in soil, increase soil nutritional benefit bacterium, and favourable crop pricks deep root, reduces soil Pass disease, reduce crop be short of seedling, the generation of dead seedling and subterranean pest-insect, there is fertilizer synergist function, improve various fertilizer utilizations Rate, phosphorus, the potash fertilizer being deposited in soil are decomposed, improve utilization rate of fertilizer.Nitrogen-fixing bacteria are to utilize the active bacteria with nitrogen fixation Plant the gaseous nitrogen in fixed air and be translated into the ammoniacal nitrogen for being available for fruits and vegetables to absorb, efficient-decomposition released mineral and soil Potassium, phosphorus in earth, the ability of several physiological active substances is produced, stimulate fruits and vegetables to give birth to by secreting vitamins and auxin substance Length, to improving soil texture, enhancing soil fertility, promoting fruits and vegetables growth, raising fruit-vegetable quality to play an important roll.Silicate is thin Bacterium can discharge the elements such as phosphorus in the rock forming mineral being made up of silicate, potassium, silicon, directly feed fruits and vegetables growth and utilize, while also With nitrogen fixing capacity, silicate bacteria will can be contained on the one hand due to organic acid substance caused by its growth metabolism in soil The slightly solubility potassium and phosphorus of the mineral such as the feldspar of potassium, mica, apatite, ground phosphate rock are dissolved out and utilized in itself for fruits and vegetables and thalline, The potassium being rich in thalline is absorbed by fruits and vegetables again after bacterium death；On the other hand the material such as hormone, amino acid, polysaccharide caused by it Promote the growth of fruits and vegetables；Meanwhile bacterium breeds in soil, suppress the growth of other pathogens.Paecilomyces lilacinus, there is height The features such as effect, wide spectrum, long-acting, safe and pollution-free, noresidue, it can substantially mitigate a variety of fruits and vegetables root-knot nematodes, SCN, stem The harm of the nematodiasis such as nematode, can the growth of obvious stimulation fruits and vegetables；Paecilomyces lilacinus is pale purple to the inhibiting mechanism of root-knot nematode After Paecilomyces varioti contacts with nematode egg capsule, in adhesive matrix, biocontrol microorganisms mycelia surrounds whole ovum, and mycelia end is thicker, due to outer The activity of source property metabolin and mycosin enzyme ruptures chorion top layer, and subsequent fungi invades and replaced, can also secrete Toxin plays toxic action to nematode.Bacillusmusilaginosiengineering, i.e. silicate bacteria, the confession of soil phophorus potassium can be effectively promoted Give, the yield of fruits and vegetables can be effectively improved；First, bacillusmusilaginosiengineering is a kind of high-active bacteria, the flourish in soil, Fixed nitrogen, phosphorus decomposing can be played and discharge effect micro- in the calcium of solubility, sulphur, magnesium, iron, molybdenum, manganese etc., not only can be with Promote the fertility of soil, the absorbable full nutrition element utilized can also be provided for fruits and vegetables, chemical fertilizer utilization can be effectively improved Rate；Secondly, bacillusmusilaginosiengineering can also produce red toxin, heteroauxin, cell after being manured into soil in its metabolic process A variety of physiologic agents such as mitogen and protein amino acids material, can increase fruits and vegetables chlorophyll content 15%-20% on year-on-year basis, It can significantly increase the photosynthesis of fruits and vegetables, promote fruits and vegetables well developed root system and robust growth, it is the drought resisting of enhancing fruits and vegetables, cold-resistant, disease-resistant And anti-adversity ability, fruits and vegetables yield can be effectively improved, product quality can also be improved；Again, this bacterium can also be effectively to soil Earth fills into a large amount of beneficial microorganisms, and profitable strain is formed in fruits and vegetables root, and can effectively suppress harmful and pathogenic micro- life The breeding of thing, the generation of a variety of soil-borne diseases, such as powdery mildew, damping-off, yellow droop etc. are substantially reduced, and then reduce part The use of agricultural chemicals, mitigate pollution by pesticides；Finally, after being manured into soil, it can constantly be decomposed for fruits and vegetables and in right amount various are provided Nutrient, effectively can prevent and improve the physiological nutritional deficiency lesion of fruits and vegetables, and these lesions include leaflet, yellow leaf, early stage Fallen leaves phenomenon, using the generation that can effectively reduce these lesions later；For fruits and vegetables class, it is strong but not can effectively to reduce gesture It is prosperous, ensure that fruit face is clean, sugariness improves, and nutrient quality significantly improves；Bacillusmusilaginosiengineering is all suitable for for various fruits and vegetables, is A kind of very efficient biological bacteria, soil characteristic can be effectively improved, promote the absorption of fruit vegetable nutrient element.Bacillus has Stronger moisture retention, bacillus can form the poly- Vetsin of the more excellent natural material of intensity, can give birth to be formed in soil One diaphragm, avoids the loss of moist in fertilizer and soil；Bacillus has stronger organic matter decomposition ability；Gemma Bacillus can discharge the high amylase of expression activitiy while increment, can be by the location resolution of some macromoleculars into energy The material for the small molecule being enough utilized；Bacillus can play the work for suppressing growth and breeding to a variety of harmful bacterias and pathogen With.Containing Bacillus cercus and gluey bacillus, as the bacterial manure of strain, the feeder capability of soil can be improved, increase fruit The secondary radical of vegetable, root activity is improved, promote the nutrient absorption of fruits and vegetables, improve nutrient P, K, Ca, Mg etc. of fruits and vegetables blade It the content of element, can also increase blade Determination of Chlorophyll content, increase the leaf-area coefficient of fruits and vegetables, improve fruits and vegetables resistance and disease-resistant Ability.Aspergillus oryzae can intensive decomposition protein, cellulose, hemicellulose, lignin etc., and by thermophilic, thermoduric bacteria, true Bacterium, yeast strain and correlation down enzyme are combined, and its living bacteria count content is high, and degradation capability is strong；In suitable condition Under, the carbon in windrow, nitrogen, phosphorus, potassium, sulphur etc. can be decomposed into mineralising rapidly, simple organic be formed, so as to be further broken into making The absorbable nutritional ingredient of thing；Aspergillus oryzae can be such that the elements such as organic matter contained in stalk and phosphorus, potassium turn into needed for plant growth Nutrition, and produce a large amount of beneficial microbes, stimulate crop production, improve the soil organism, improve soil texture；Supplement soil Middle quantity of useful microbe, further promote matter and energy in soil to convert, the formation and decomposition of humus, improve fertilizer profit With rate, beneficial metabolites are produced, suppresses and kills harmful bacteria.Organic substance in soil is decomposed or dissolved to bacillus megaterium, and The P elements being easily absorbed by plants are changed into, improve the utilization rate of P elements；Bacillus megaterium can breed rapidly in soil, As dominant bacterias, nutrition and other resources of rhizosphere are controlled, cause pathogen to lose living space and condition to a great extent, Plant related organization cell wall thickening, fibrosis, degree of lignification are improved, and the double silicon layers of cutin, shape are formed outside epidermis Into the barrier for preventing germ to attack together, Fertilizer application rate is improved, promotes plant absorption nutrient, improves fertilizer efficiency, it, which is metabolized, produces Poly- Vetsin have stronger water conservation, fertilizer-preserving ability, controlling plant diseases occur.The biological flora that soil-repairing agent includes is huge Bacterium anthracoides, nitrogen-fixing bacteria, colloid bacillus cereus, aspergillus oryzae, long handle trichoderma can in plant root outgrowth, these have prebiotic Thing flora growth forms symbiosis in the root of subject plant, and with subject plant, can not only promote the life of root system of plant It is long, required nutritional ingredient is provided for it, while plant also provides condition for the growth of beneficial bacterium, root is beneficial with these The plant of biological flora can preferably absorb phosphorus, and strengthen its salt resistance, Hangzhoupro drought, keep the ability of water balance so as to promoting to plant Thing growing in itself；Vegetable Lay, fruit tree, great Jiang, strawberry etc. have the plant of symbiosis in resistance to soil with beneficial organism flora High temperature, regulation pH value, the infringement of anti-pathogenic bacteria and preventing from heavy metal toxicity play a role significantly.
Herbicide, chemical fertilizer, agricultural chemicals, fruits and vegetables can effectively be decomposed certainly using the soil-repairing agent containing bacteria agent of the present invention The residual toxins such as poison, fruits and vegetables root growth caused by releasing toxin is bad, activates soil enzyme, improves hardened soil zwitterion knot Structure, balanced soil acid-base value, soil permeability is improved, good living environment is provided for fruits and vegetables；Repair due to soil degradation (battalion Support balance) caused by various root problems, block to it is susceptible tissue supply nutrition, help fruits and vegetables balance own metabolism system, keep away Exempt from and reduce due to physiological disease caused by metabolic system disorder.
Above-mentioned method, it is preferred that by weight, the agricultural amino acid original powder includes proline 1-2 parts, glycine 1-2 parts, nitrogen propylhomoserin 1-2 parts, tryptophan 0.5-1 parts, cysteine 1-2 parts and alanine 0.5-1 parts.
Above-mentioned method, it is preferred that the biological organic fertilizer is mainly by straw (the predominantly cotton of 30-50 parts by weight Flower, rice, corn, wheat stalk, branch crushed material), turf 5-10 parts by weight, 5-10 parts by weight cakes powder (predominantly beancake powder, The mixture of bean cake powder, groundnut meal), 5-10 parts by weight cake-shaped rape seed dregs cake, 5-10 parts by weight animal bone powder (predominantly oyster shell whiting, fish Bone meal), the calcium superphosphate of 1-2 parts by weight, the humic acid of 2-5 parts by weight, the middle micro- (moderate-element of 0.1-1 parts by weight Content calcium >=10%, magnesium >=2%, sulphur >=2%；Micronutrient levels boron, copper, iron, manganese, molybdenum, zinc >=0.2%) mixing it is laggard Row aerobic compost, through abundant fermentation maturity, the compound bacteria C of addition 2-3 parts by weight is obtained；The compound bacteria C includes following raw material： Photosynthetic bacteria spore >=40 × 109Cfu/g, saccharomycete spore >=40 × 109Cfu/g, cellulase (u/g) >=1,000,000,000/g, protease (u/g) >=1,000,000,000/g, amylase (u/g) >=40 × 109Cfu/g, pectase >=40 × 109Cfu/g, hemicellulase >=40 × 109Cfu/g, agricultural amino acid original powder mass fraction >=2.0%, spore phosphorus >=6 × 109Cfu/g and spore potassium >=6 × 109cfu/ g。
Above-mentioned aerobic compost course of reaction is：The agitated machine of each raw material is sufficiently mixed rear aerobic compost, it is good through high temperature Oxygen compost, temperature control, under conditions of aerobic, are borrowed using the every 10-12 hours aeration of air compressor once at 60~80 DEG C The effect of aerobic microbiological in air is helped to carry out；In composting process, dissolved organic matter matter passes through the cell of microorganism Wall and cell membrane are absorbed for microorganism, and the organic matter of solid and colloid is first attached to outside microbial body, by Institute of Micro-biology's secretion Ectoenzyme penetrates into cell again after being decomposed into dissolved matter；Vital movement-oxidation, reduction and the synthesis that microorganism passes through itself Process, a part of oxidation operation into simple inorganic matter, ATP energy is discharged, and the conversion of a part of organic matter is made a living Nutriment necessary to object is to synthesize new cellular material, and then microorganism species are grown into breeding and produce more lifes Object；Simultaneously under this fermentation temperature, various pathogens, parasitic ovum, weed seed etc. can be killed, and reduce pest and disease damage hair It is raw.
Organic fertilizer after fermentation adds the content of the soil organism, can be reduced root system with chesson and entered between soil Resistance, reduce root system allelochemical (suppression harmful bacteria) enter soil, including the secondary metabolites such as phenols, terpene, alkaloid Fruits and vegetables are influenceed from poison.
Compound bacteria C is added after the completion of fermentation, is sufficiently mixed, and the collective effect of compost raw material, forms that quantity is big, kind Enzyme more than class, these enzymes have synthesized the material similar to fruits and vegetables root exudates composition in soil biochemistry reaction, these Material can retain wherein as the presence of fruits and vegetables resisting substance, the metabolite of microorganism, meet that fruits and vegetables resist stress；Fruits and vegetables In whole breeding cycle, once run into stress, it will mitigate stress by way of itself discharging some resisting substances.
Cellulase acts on the processing of stalk and organic substance, has synergy with pectase and hemicellulase, Straw plants cell membrane is destroyed, promotes the release of intracellular active principle, improves stalk corruption dynamics, increase organic fertilizer pol Content；Cellulose degraded chitosan production low-molecular weight chitoglycan, protease can be by macromoleculars under certain temperature, pH value Protein is hydrolyzed into the products such as polypeptide and amino acid, is supplied directly to fruits and vegetables absorption, promotes growth, improves immune.Amylase energy Starch is acted on, final product is glucose, is advantageous to fruits and vegetables and absorbs, and improves immune, increase yield.Spore phosphorus, spore Potassium, the deficiency of the nutrients such as the potassium of crops, magnesium, phosphorus, silicon can not only be augmented after, moreover it is possible to various bioactivators are produced, Promote crop growth, strengthen crop cold resistance drought resisting and resist the ability of pest and disease damage, rate of growth is all more than 15%；When A series of enzyme is produced after potassium, phosphobacteria and mineral contact, mineral matter crystalline texture is destroyed, has in powerful activating soil The ability of the ash elements such as potassium, phosphorus, magnesium, iron, silicon, so as to discharge nutrient；Second, B. mucilaginocus and the contact of rock forming mineral matter surface Afterwards, ion exchange is carried out, potassium is discharged, and organic acid can be secreted, the potassium of slightly solubility, phosphorus are converted into water miscible Potassium, phosphorus；B. mucilaginocus can also secret out of the physiologically active thing for stimulating plant growth, strengthen drought resisting, the cold tolerance of crop, and to one A little plant disease such as wheat rust, sclerotinia sclerotiorum have certain inhibitory action.
And several enzyme activity employed in compound bacteria C are all market products, are used in mixed way the effect for reaching sustained release, can be had Effect overcomes the problem that microbial bacteria, composite fertilizer can not mutually melt.Compound bacteria mycorhiza adds the surface of fruits and vegetables Root Absorption phosphorus, makes fruit Vegetable can absorb original unserviceable phosphorus source, and be allowed to convert, be conveyed to host's fruits and vegetables；Mycorhiza changes the growth shape of fruits and vegetables State, promote the activity raising of rhizosphere acid phosphatase and alkaline phosphatase；Mycorhiza expands root circle scope, also just expands phosphorus Consumption circle humic acid has improvement fruits and vegetables rhizosphere microorganism community diversity, improves fruits and vegetables root activity, extends root circle activity model Enclose, add the surface area of fruits and vegetables Root Absorption fertilizer, promote fruits and vegetables root system nutrition to absorb, resisted by strengthening fruits and vegetables itself Power, reach the purpose for the Resistant for improving fruits and vegetables.The spore exo polysaccharides that compound bacteria C is overflowed are the adhesives of soil granular structure, Can chesson, strengthen soil granular structure, improve retain water and nutrients ability, increase the soil organism, it is potential in activating soil Nutrient, effectively improve Cultivated-land Fertility, improve fertilizer provision from soil environment, promote the propagation of fruits and vegetables beneficial rhizosphere microorganisms, improve fruits and vegetables Rhizosphere microorganism Community Ecology environment, the increase of beneficial microbe group and fruits and vegetables disease-resistant factor, also can obviously reduce soil-borne disease Harmful infects, and reduces the incidence probability of continuous cropping fruits and vegetables, improves fruit-vegetable quality and yield.
The biological organic fertilizer of the present invention is supported since root, supports root mulch, and root system is that fruits and vegetables absorb nutrition, conveys the weight of nutrition Organ is wanted, the exploitation and protection of root system are most important, healthy and strong flourishing root system, promote fruits and vegetables plant to absorb supporting in soil very well Point and nutriment, reduce the probability of fruits and vegetables infection pest and disease damage, effectively improve the high yield, high-quality of fruits and vegetables.The biology of the present invention has Machine fertilizer can promote the growth of beneficial microbe colony in soil, accelerate the metabolic efficiency of root system, adjust Enzyme Activities of Root System, promote thin Born of the same parents divide, and accelerate root growth, effectively supplement fruit tree energy, increase soil porosity, improve soil granular structure, suppress harmful Flora, prevent the generation and dip-dye of a variety of soil-borne diseases；Applied fertilizer within the growth period of fruits and vegetables during top dressing, it is endogenous tree body can be suppressed Bacterium, reduce the generation of canker.
Above-mentioned method, it is preferred that in the step (5), immune vaccine sprayed once every 15-20 days.
Above-mentioned method, it is preferred that in the step (1), sprayed using 300-400 times of dilution gardenful of soil-repairing agent Apply 1-2 times.
Specifically in implementation process, when disease occurs for plant, the first water of 5 kilograms or so of note in sprayer, it will exempt from 300-400 times of dilution of epidemic disease vaccine sprays blade face, is advisable with plant leaf whole humidity.It is whole by our many experiments System pest and disease damage controllable rate is more than 90%, can be with if pest and disease damage occurs caused by the factors such as environment, weather, administrative skill Using green prevention and control technology：According to per hectare installation frequency conversion trapping lamp 1-2 small cups, put up yellow plate 1000-1500, backboard 1000- 1500, the innovative plant source put on record using sugar-vinegar liquid transfusion bottle 500-600, using other and biological source noresidue agriculture Medicine, it is ensured that agricultural product and Environmental security.
Immune vaccine, soil remediation have been used in the method without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage of the present invention Agent, biological organic fertilizer, soil-repairing agent, bio-organic fertilizer product have the non-pathogenic beneficial microbe colony of high concentration, bag Bacillus coagulans, bacillus megaterium, azotobacter vinelandii, colloid bacillus cereus, aspergillus oryzae, long handle trichoderma are included, these are beneficial Biological flora can form symbiosis with plant, promote plant root growth, while protection plant fully absorbs nutrient, plant Also nutrient is provided for, stimulates enzyme activity, there is provided it is immune, resist disease.Profitable strain energy in soil-repairing agent, biological organic fertilizer Nutrient is absorbed from soil and decomposes the nutrient that the multiple and material generation fruits and vegetables in soil can be utilized directly, is especially absorbed Potassium and phosphorus, these Nutrient retranslocations are then carried out into the more plain underdeveloped deficiency of root system to plant, so as to promote the growth of root system and rise To fixed nitrogen, promote plant growth and protect plant not acted on by pathogenic bacteria infringement.When fruits and vegetables are by environmental stimuli or in inverse During the condition of border, immune response can be produced by the defence and metabolic system for adjusting itself, this defense reaction of fruits and vegetables or exempted from Epidemic disease Resistant reaction, fruits and vegetables can be made to postpone or mitigate the occurrence and development of disease.
The method without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage of the present invention is right using soil-repairing agent rehabilitating soil Soil disinfection, by various organic fungi-manure fertilizer, increased fertility of land level, balance fertilizing, and an optimal growth life is created to fruits and vegetables State environment, lifting fruits and vegetables root system convert to the absorption efficiency of liquid manure and nutrition, improve crop；Spray on plant immune vaccine blade face Apply, promote growth, improve resistance.Fruit tree leaf is the most important breathing of plant and photosynthetic organ, is carry as fruit tree Growth continuously feeds the function of nutrient energy in a steady stream.Foliage-spray is carried out using plant immune bacterin preparation, fruit can be adjusted Vegetable grows, and strengthens leaf photosynthesis ability, the remarkable efficacy for promoting fruit tree growth gesture to recover；Good film forming, it can plant Strain blade face forms diaphragm, blocks the propagation of germ, effectively plays the effect of pre- disease prevention.
Disease resistance of plant is the product of plant and microorganism common evolutionary, and the present invention one is long-term three-dimensional from timing node Chemoprevention is controlled, and whole preventing and treating is before whole fertility, from seed to harvest and whole growth period；It is second, three-dimensional to fruits and vegetables different parts Chemoprevention is controlled, and pest and disease damage can effectively be killed by spraying the root in fruits and vegetables, skin (vascular bundle), stem, fruit；Third, three-dimensional in usage Preventing and treating, two effects of immunity and sterilization are improved come controlling disease by rejuvenation, and with soil-repairing agent, biological organic fertilizer reparation Soil balanced soil fertilizer efficiency, it is the three-dimensional approach for realizing two effects, due to activator in bio-bacterial manure and fertilizer conservation synergist Double action, improve product quality, reduce harmful substance accumulation.
The present invention the method without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage, mainly include immune vaccine immersion seed, Matrix sterilization, soil disinfection, foliage-spray, balanced soil fertility of improving the soil, fertilising optimization beneficial microbe colony, physics prevention and control, The contents such as biological protection, be on the basis of analysis fruits and vegetables, harmful organism and environmental factor three's correlation comprehensively, it is comprehensive With various agricultural measures, the quantity of harmful organism is forced down, itself resistance and immunity increase is induced, improves fruits and vegetables resistance, Create and be advantageous to the farmland ecological environment that fruits and vegetables grow and are unfavorable for harmful organism generation, directly or indirectly eliminate or press down Method with harm occurs for harmful organism processed, is a kind of most economical, most comprehensive prevention and controls.The present invention's is comprehensive without agricultural chemicals solid The method for closing prevention and control fruits and vegetables pest and disease damage, is the integrated innovation for strengthening green prevention and control technical system, and optimization is supporting various effectively green Prevention and control key technology.
Compared with prior art, the advantage of the invention is that：
(1) method without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage of the invention so that whole ecological prevention and control systemic disease Insect pest controllable rate is more than 90%.
(2) method without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage of the invention, whole process is without applying pesticides, green ring Protect.
(3) method without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage of the invention, immune vaccine is sprayed to fruits and vegetables, is suppressed Infection process, growth, the raising of fruits and vegetables autoimmunity and the resistance to environmental pressure are stimulated, stimulate and regulation fruits and vegetables are quick Growth, promote fruits and vegetables well developed root system；Absorption of the fruits and vegetables to nutriment is improved simultaneously, strengthens the metabolic function of fruits and vegetables, improves light Cooperation is used, and improves crop yield, lifts product quality.
(4) method without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage of the invention, can activate fruits and vegetables defense mechanism, carry High fruits and vegetables adapt to the ability of adverse circumstance (cold, arid and disease etc.) and resist the ability of stress factors, effectively prevent low-temperature frozen Evil and pest and disease damage, substantially reduce the occurrence probability of fruits and vegetables pest and disease damage.
Brief description of the drawings
Fig. 1 and Fig. 2 is the present invention using pepper plant in the procedure without agricultural chemicals stereo synthesis prevention and control capsicum pest and disease damage Configuration.
Fig. 3 is the present invention using pepper plant blade in the procedure without agricultural chemicals stereo synthesis prevention and control capsicum pest and disease damage Situation.
Fig. 4 is health of the present invention using pepper plant in the procedure without agricultural chemicals stereo synthesis prevention and control capsicum pest and disease damage Situation.
Fig. 5,6,7,8,9 and 10 are that method of the use without agricultural chemicals stereo synthesis prevention and control capsicum pest and disease damage of the present invention obtains Pepper fruit situation.
Embodiment
For the ease of understanding the present invention, below in conjunction with Figure of description and preferred embodiment to invent herein do it is more complete Face, meticulously describe, but protection scope of the present invention is not limited to specific examples below.
Unless otherwise defined, all technical terms used hereinafter are generally understood that implication phase with those skilled in the art Together.Technical term used herein is intended merely to describe the purpose of specific embodiment, is not intended to the limitation present invention's Protection domain.
Unless otherwise specified, various raw material, reagent, the instrument and equipment etc. used in the present invention can pass through city Field is commercially available or can be prepared by existing method.
Employed in following examples to immune vaccine in Contents of Main Components be：Biologically active polypeptide >=50g/ L, chitosan oligosaccharide >=50g/L, amino acid >=50g/L, sucrose water >=20g/L, compound bacteria A >=50 × 109cfu/g；The compound bacteria A Refer to beauveria bassiana spore >=50 × 109Cfu/g, Trichoderma spore >=50 × 109Cfu/g, saccharomycete spore >=50 × 109cfu/ G, photosynthetic bacteria spore >=50 × 109Cfu/g, muscardine spore >=50 × 109Cfu/g compound bacteria；Used soil-repairing agent In main component include：Biologically active polypeptide >=100g/L, agricultural amino acid original powder mass content >=2.0%, compound bacteria B >= 100×109cfu/g；Wherein described compound bacteria B is the flora for including following strain：Bacillus globigii spores 2 × 109cfu/g、 Actinospore >=40 × 109Cfu/g, saccharomycete spore >=40 × 109Cfu/g, nitrogen-fixing bacteria spore >=40 × 109Cfu/g, silicon Hydrochlorate bacterium spore >=40 × 109Cfu/g, Paecilomyces lilacinus spore >=40 × 109Cfu/g, bacillusmusilaginosiengineering compound bacteria / gram of spore >=500,000,000 ,/gram of photosynthetic bacteria spore >=5,000,000,000, beauveria bassiana spore >=40 × 109Cfu/g, Trichoderma spore >=40 ×109Cfu/g, Bacillus cereus spores >=4 × 109Cfu/g, gluey subtilis spore >=4 × 109Cfu/g, aspergillus oryzae Spore >=4 × 109Cfu/g, bacillus megaterium spore >=4 × 109cfu/g；Used biological organic fertilizer is mainly by 40 parts by weight Straw, the parts by weight of turf 8,8 parts by weight cake powder, 8 parts by weight cake-shaped rape seed dregs cakes, 8 parts by weight animal bone powders, 1.5 parts by weight Calcium superphosphate, the humic acid of 4 parts by weight, the middle trace element of 0.7 parts by weight carry out aerobic compost after mixing, rotten through fully fermentation Ripe, the compound bacteria C of 2.5 parts by weight of addition is obtained；Compound bacteria C includes following raw material：Photosynthetic bacteria spore >=40 × 109Cfu/g, ferment Female bacterium spore >=40 × 109Cfu/g, cellulase >=1,000,000,000/g, protease >=1,000,000,000/g, amylase >=40 × 109Cfu/g, fruit Glue enzyme >=40 × 109Cfu/g, hemicellulase >=40 × 109Cfu/g, agricultural amino acid original powder mass fraction >=2.0%, spore Phosphorus >=6 × 109Cfu/g and spore potassium >=6 × 109cfu/g。
Embodiment 1：
The method without agricultural chemicals stereo synthesis prevention and control pest and disease damage of the present invention is applied on plantation capsicum, observes it to capsicum Insect pest and the influence of Yield and quality.
First, model field (model field area：3 mu；Capsicum variety：Long capsicum；Plant spacing：1200 plants/acre, test period： On November 20,15 days -2015 January in 2015) handled in the following way, comprise the following steps that：
(1) 7 days before pepper planting, sprayed 1 time using 300-400 times of dilution gardenful of soil-repairing agent, soil is repaiied The dosage of multiple agent is 2 kgs/acre；
(2) in the pepper seedling raising phase, substrate soil is repaired using 300 times of soil-repairing agent of dilution；
(3) before pepper seedling plantation, capsicum root 0.08h is soaked using 2000 times of dilutions of immune vaccine；
(4) within the growth period (seedling replanting phase-picking fruit phase) of capsicum, applied fertilizer using biological organic fertilizer；
(5) within the growth period (seedling replanting phase-picking fruit phase) of capsicum, except full-bloom stage sprayed every 15-20 days 1 immune vaccine, remaining growth phase is every 15-20 days foliage-sprays 1 time；Wherein Seedling Stage immune vaccine diluted concentration 500 Times, remaining growth phase dilutes 300 times, is stopped using within 20 days before the picking fruit phase.
2nd, field (area is compareed：3 mu；Capsicum variety：Long capsicum；Plant spacing：1200 plants/acre), control field is according to routine Mode carries out production management.
3rd, plant performance and fruit yield, the attributional analysis of model field are observed：
1st, model field plant shows：
(1) seedling stalwartness survival rate is high, and for transplanting survival rate up to 100%, transplanting seedling time is short, 3-5 days can slow seedling, growing way It is prosperous.
(2) Adult plant plant leaf is dark green, and growing way neat and consistent, form is good, and invalid branch tiller is few, seat flower percentage of fertile fruit Height, result quantities are big, and pepper shape is good, not only straight but also suitable, and color and luster orthodox school is beautiful.
2nd, the immune performance of model field plant：
(1) the disease-resistant pest-resistant anti-adversity ability of model field plant is high, and it is 0 to grow whole plant diseases incidence, suppresses to decompose kind Seedling carries virus, the complete virus-free diseased plant of canopy.
(2) plant forms are good, and invalid branch tiller is few, and growing way is good, neat and consistent, whole without diseased plant, sick leaf, disease fruit, such as Shown in Fig. 1 and Fig. 2；Leaf dark green is glossy, and wax coat is abundant uniform, as shown in Figure 3；Plant strain growth health, secondary metabolites It is abundant, as shown in Figure 4；Plant seat flower percentage of fertile fruit is high, and pattern is beautiful, and fruit shape is beautiful, and straight suitable, length is basically identical, is advantageous to wrap Defeated and standardized production is shipped, as shown in Fig. 5,6,7,8,9 and 10.
(3) pepper fruit flavor substance enriches, in good taste, and fruit cell health degree is high, than great, resistance to accumulating.
3rd, yield and quality performance：
(1) yield shows：Whole harvesting terminates in season, and every plant of capsicum average product of model field is 11 jin, and per mu yield= 11*1200=13200 pounds/acre；Local 6 jin of capsicum average product of every plant of Routine Management one, per mu yield=6*1200= 7200 pounds/acre；Model field capsicum increases production ratio=(13200-7200)/7200=83%.
(2) model field Quality of Capsicum：Whole process plantation is without disease pest, without using any bactericide, insecticide, zero agriculture is residual, nothing Public hazards；Capsicum flavor substance foot, it is in good taste, it is loved by consumers, the capsicum of model household realizes order sale；Resistance to accumulating, Be advantageous to be peddled at a distance and long-time storage.
(3) economic benefit：This season kind capsicum average market purchasing price is 1.5 yuan/jin, and model field can increase the output value =(13200-7200) * 1.5=9000 members/mu, 3 mu of model field area, increase economic benefit altogether：9000*3=27000 members.
Embodiment 2：
The method without agricultural chemicals stereo synthesis prevention and control pest and disease damage of the present invention is applied on plantation grape, observes it to grape Insect pest and the influence of Yield and quality.
Experimental design：Contrast test is in 2 months 2012-2 months 2015 in Hunan Province Chenzhou City Institute of agricultural sciences fruit Carried out in the grape base of vegetable base demonstration garden, cropping pattern sets A, B group, and A groups use the technology of the present invention cultivation mode, and B groups are Common cultivation management mode.It is 5 years raw graftings to try material, and huge peak, sunlight rose, summer are black, the simple rain shelter cultivation of greenhouse, 2 months First arrival overlay film in June is taken shelter from rain, south-north direction, canopy long 50.0m, wide 2.0m, the high 2.4m of canopy, pergola cultivation, seeding row spacing be 1.5m × 3.0m。
The step of specific plantation grape, is as follows：
(1) before wine-growing 10 days, 2 times are sprayed using 300 times of dilution gardenfuls of soil-repairing agent, rehabilitating soil；
(2) in wine-growing early stage, whole strain grape seedlingses are soaked 0.05 hour using 2000 times of dilutions of immune vaccine；
(3) in grape nursery stage, substrate soil is repaired using 300 times of dilutions of soil-repairing agent；
(4) within the growth period of grape, applied fertilizer using biological organic fertilizer, apply fertilizer every plant of initial stage 0.5kg, every when bearing fruit 2.5kg is applied in strain；
(5) within the growth period of grape, whole strain is sprayed using 500 times of dilutions of immune vaccine, 15-20 days once.
Because sprouting stage has many pest damage young shoots and tender leaf, when grape bud 1cm or so is long, by plant immune prevention and control vaccine The whole strain of 500-800 times of liquid of preparation diluent sprays, and 15-20 days once, improves grapevine seedling immunity；Pass through experiment, whole system Pest and disease damage controllable rate is more than 80%, and pest and disease damage occurs caused by the factors such as environment, weather, administrative skill, can use green Control Technology：According to per hectare installation frequency conversion trapping lamp 1-2 small cups, put up yellow plate 1000-1500, backboard 1000-1500, make With sugar-vinegar liquid transfusion bottle 500-600, the innovative plant source and biological source nonpersistent pesticide put on record using other, it is ensured that agriculture Product and Environmental security.
Fruit quality determination method：After fruit maturation, each cell of different planting takes 3 braids to go back to laboratory at random, exists side by side Carry out quality, volume analysis.
Phenological period is investigated：From contrast, under 2 kinds of different plantings, the main phenological period of the grape of Routine Management Obvious difference be present.Using under cultivation condition of the present invention, budding period, full-bloom stage, fruit color phase, the maturity period of grape Shift to an earlier date 5,7d, 5d, 8d than common cultivation respectively.
Fruit quality is investigated：In fruit endoplasm, soluble solid content under cultivation condition of the present invention and solid sour ratio are used Pole is significantly higher than conventional cultivation mode, and acid content pole is substantially less than conventional cultivation mode, and grape mouthfeel pole sweet tea is in conventional cultivation, wind There is pole significant advantage on taste.
Major Diseases a situation arises investigation：Anthrachose of grape, downy mildew and powdery mildew are the multiple diseases of grape, are serious shadows Ring one of principal element of fruit yield, quality and economic benefit.In planting type, planting type downy mildew of the present invention, grey mold Disease, leaf spot, spot disease, ring spot, fruit-shrink disease, anthracnose, anthrachose of grape, the incidence of disease of powdery mildew and insect pest disease index are aobvious Work is less than conventional cultivation, and prevention and control index reaches more than 90%.
Economic benefit and social benefit：The grape fruit maturity period in advance and quality raising, not only make fresh fruit listing morning, Demand of the people to high-quality Table Grape is met, and is brought a high price.Under cultivation condition of the present invention, sunlight rose uva Real price is 60 yuan/kg or so, and it is 20 yuan/kg that the summer is black, huge peak for 16 yuan/kg；Under the conditions of conventional cultivation, sunlight rose Portugal Grape fruit price is 20 yuan/kg or so, and it is 10 yuan/kg that the summer is black, huge peak for 4 yuan/kg；The output value of 2 kinds of planting types is than being then 3 : 2: 4, economic benefit and social benefit significant difference.
Grape is planted using the method without agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage of the present invention, not only economic benefit shows Write, and conventional pesticide usage amount is almost nil, non agricultural chemical residuum, and agricultural product quality improves, and significantly improves ecology and society Benefit.Scientific research demonstration result carries out analysis shows, and up to more than 90%, control effect reaches demonstration area green prevention and control technology arrival rate 90%, reduce chemical pesticide and use more than 99%, 30% is averagely reduced per 667m2 cost accountings.The control of pest and disease damage loss late is arrived 3%~5%.Radiation drives area's green prevention and control technology arrival rate also up to more than 60%.With applying chemical fertilizer and chemical pesticide phase Than that nitrate content in product can be made to reduce by 20%~30%, VC contents raising 20%~30%, soluble sugar can improve 1~4 Degree.Fresh keeping time is long, Storage period extends more than 15 days.Grape product taste is more preferable.
Embodiment 3：
The method without agricultural chemicals stereo synthesis prevention and control pest and disease damage of the present invention is applied on plantation citrus, observes it to citrus Insect pest and the influence of Yield and quality.
First, experimental design：Tested in January, 2016-2017 year January in Hunan Province Chenzhou City Institute of agricultural sciences mandarin orange Tangerine Demonstration Garden is carried out.Select two emperor mandarin orange (tribute mandarin orange), crystal sugar orange kinds.3 × 3.5m of emperor mandarin orange plant seeding row spacing (63 plants/ Mu)；3 × 3m of crystal sugar orange (76 plants/acre), soil pH≤8.1, thickness of soil is in more than 1.20m, and level of ground water is in below 1.20m. Citrus orchard demonstration cultivated area l5 mus.Cropping pattern sets A, B group, and A groups use the technology of the present invention cultivation mode, and B groups are common Cultivation management pattern.
2nd, tangerine seedling is cultivated and is colonized
(1) 10 days before emperor mandarin orange, crystal sugar orange plantation, sprayed 2 times using 300 times of dilution gardenfuls of soil-repairing agent, Rehabilitating soil；
(2) early stage is planted in emperor mandarin orange, crystal sugar orange, is soaked 0.05 hour using the whole strain of 1500 times of dilutions of immune vaccine；
(3) in emperor mandarin orange, crystal sugar orange nursery stage, substrate soil is repaired using 300-400 times of dilution of soil-repairing agent；
(4) in emperor mandarin orange, crystal sugar orange result initial stage and Seedling Stage, sprayed using 400 times of dilution gardenfuls of soil-repairing agent 1 time is applied, rehabilitating soil；
(5) emperor mandarin orange, crystal sugar orange growth period in, applied fertilizer using biological organic fertilizer, as a result applied every plant of initial stage 0.5kg, bear fruit tree when every plant apply 2kg；
(6) emperor mandarin orange, crystal sugar orange growth period in, spray whole strain, 15-20 using 500 times of dilutions of immune vaccine It is once.
The main task of the first fruiting period of emperor mandarin orange, crystal sugar orange is to improve yield as early as possible, improving quality, while continues to expand tree Hat, root system, pre- preventing disease and pest；The best fruiting period will take Optimum Synthesis to prevent and treat orange disease and insect pests, be exactly strong in scientific management, culture Strong tree vigo(u)r, on the basis of pest and disease damage ability is resisted in enhancing, by comprehensive preventive health measures, according to pest and disease damage Economic Index of Controlling, close Medication fertilising is managed, is prevented and treated in good time according to methods such as pest and disease damage predictions, both can reach and reduced medication, reduce cost, reduce agriculture The purpose of medicine residual, and can reach preferable prevention effect.
3rd, citrus harvests
It is average 130 using the method for the present embodiment, emperor's mandarin orange per plant number；Single fruit weight is average 155 grams；Single fruit footpath 7.0cm；Single plant yield is average 21 kilograms, compared with the emperor tribute mandarin orange rate of growth more than 12% of common management, prevention and control of plant diseases, pest control rate 90% More than.
It is average 145 using the method for the present embodiment, crystal sugar orange per plant number；Single fruit weight is average 122 grams；Single fruit footpath 6.6cm；Average 26 kilograms of single plant yield, compared with the crystal sugar orange rate of growth more than 15% of common management, prevention and control of plant diseases, pest control rate 90% with On.
Embodiment 4：
The method without agricultural chemicals stereo synthesis prevention and control pest and disease damage of the present invention is applied on plantation tomato, observes it to tomato Insect pest and the influence of Yield and quality.
Experimental design：Experiment in cultivation is contrasted 2 months in October, -2016 in 2016 in Hunan Province's Chenzhou City research of agricultural science Carried out in the fruits and vegetables base of institute's fruits and vegetables base demonstration garden, examination material is tomato, and seed is bought for market；Seeding row spacing 30cm × 40cm, is adopted With zanjon high ridge, every 667 ㎡ transplants 2800 plants or so.Cropping pattern sets A, B group, and A groups use the technology of the present invention cultivation mode, B groups are common cultivation management mode.
Field management：After field planting based on heat and moisture preserving, promote slow seedling.Slow seedling to florescence, general daytime is maintained at 28 ~30 DEG C, night is maintained at 15 DEG C or so.That multilayer covering is flexibly grasped depending on weather condition takes off the lid time, but to keep appropriateness logical Wind, canopy room humidity is reduced, mitigates disease.All prevention and control of plant diseases, pest control should follow the principle of " putting prevention first, integrated control ", Planted, comprised the following steps that using Control Technology of the present invention：
(1) before tomato seeds sowing, using 1500 times of dilutions of immune vaccine, seed 1-2 hours are soaked；
(2) before tomato seedling plantation, using 2000 times of immune vaccine dilution immersion tomato seedling root 0.05 hour；
(3) before tomato planting 8 days, sprayed 1 time using 300 times of dilution gardenfuls of soil-repairing agent；
(4) in the tomato seedling phase, substrate soils are repaired using 400 times of dilutions of soil-repairing agent；
(5) within the growth period of tomato, applied fertilizer using biological organic fertilizer, every plant of 0.2kg；
(6) within the growth period of tomato, the whole strain of tomato is sprayed using 800 times of dilutions of immune vaccine.
Using the technology of the present invention tomato because its quality it is extremely excellent, high and stable yield, the tomato market price is higher, is maintained at 2-4 Member/kg or so, common cultivation pattern are 1-2 members/kg, and economic benefit improves 50%；Ten thousand yuan of left sides of 15-20 are reached per 1h ㎡ benefits The right side, Cotton Varieties by Small Farming Households enthusiasm are higher.A groups fruit is circular, fruit surface smooth, and young fruit has green fruit shoulder, mellow fruit pink, is rich in light Pool, average fruit weight 13-18g or so, average soluble solid content 9.7%, reaches as high as 12%, sour-sweet moderate, wind Taste is good, and quality is extremely excellent；Pericarp is thin but toughness is strong, is not easy dehiscent fruit, and storage tolerance, malformed fruit rate is low, shelf life length.Field resistance By force, the disease such as anti-blight, ToMV (Tomato yellow mosaic virus), pest and disease damage prevention and control rate is more than 90%.
Embodiment 5：
The method without agricultural chemicals stereo synthesis prevention and control pest and disease damage of the present invention is applied on plantation water spinach, observes it to water spinach Insect pest and the influence of Yield and quality.
Experimental design：Water spinach also known as water spinach, contrast test in March, 2016 in September, -2016 in Hunan Province's Chenzhou City agriculture Carried out in the fruits and vegetables base of industry Science Institute fruits and vegetables base demonstration garden, examination material is water spinach.Live every 667 square metres of sowing quantity 12- More than 16 kilograms, seed is bought for retail sales.Cropping pattern sets A, B group, and A groups use the technology of the present invention cultivation mode, and B groups are general Logical cultivation management pattern.
The method without agricultural chemicals stereo synthesis prevention and control pest and disease damage of the present embodiment, specifically includes following steps：
(1) before the sowing of water spinach seed, using 1500 times of dilutions of immune vaccine, immersion seed 2 hours；
(2) in water spinach nursery stage, substrate soil is repaired using 300-400 times of dilution of soil-repairing agent；
(3) before water spinach is planted 8 days, sprayed 2 times using 350 times of dilution gardenfuls of soil-repairing agent；
(4) when water spinach seedling replanting is planted, using 2000 times of immune vaccine dilution immersion water spinach root 0.08 hour；
(5) within the growth period of water spinach, applied fertilizer using biological organic fertilizer, every mu of 150kg；
(6) within the growth period of water spinach, the whole strain of water spinach vegetable is sprayed using 300 times of dilutions of immune vaccine.
Immune vaccine was found the cloudy day every 15-20 days or sprayed once at dusk, such as heavy rain, needed to fill spray once every other day；Harvesting Stop spray on plants vaccine within first 7 days.
Early spring with the method for broadcasting sowing, covers 1 centimetre of the dry fine earth to have sieved thereon, is emerged with profit, then in lid mulch film thereon, and set Small plastic shed, it is closed after having 80% seed sprouting, throw off mulch film.
According to market conditions, list as early as possible.Due to the use of the technology of the present invention, it will be apparent that promote growth and the hair of water spinach Educate：First, it is vigorous to grow, plant strain growth is healthy and strong, and fruit-setting rate improves；According to investigations, the present embodiment is higher than the yield of Routine Management 20%~40%, average plant height improves 10%~15%；Second, mitigate the disease such as pest and disease damage, sun burn, virosis, anthracnose The incidence of disease, disease index substantially reduces；Meanwhile the method preventing and treating of preventing disease and pest, easy to operate, prevention effect is good, prevention and control rate More than 90%；Third, improve the yield and quality, all kinds of vegetables planted using the method for the present invention, can typically increase production 20% with On, plant good appearance, features good taste, without conventional residues of pesticides, reach green food standard, commodity property significantly improves, than normal The price for advising cropping pattern improves 10%-25%.

Claims (7)

  1. A kind of 1. method of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage, it is characterised in that comprise the following steps：
    (1) before fruits and vegetables are planted 7-10 days, using soil-repairing agent rehabilitating soil；
    (2) before fruits and vegetables seedling cultivation, fruits and vegetables root 0.05-0.08 is soaked using 1500-2000 times of dilution of immune vaccine Hour or whole strain immersion 0.01-0.05 hours；
    (3) in fruits and vegetables nursery stage, substrate soil is repaired using 300-400 times of dilution of soil-repairing agent；
    (4) within the growth period of fruits and vegetables, applied fertilizer using biological organic fertilizer；
    (5) within the growth period of fruits and vegetables, the whole strain of fruits and vegetables is sprayed using 300-800 times of dilution of immune vaccine；
    Wherein, the Contents of Main Components in the immune vaccine is：Biologically active polypeptide >=50g/L, chitosan oligosaccharide >=50g/L, ammonia Base acid >=50g/L, sucrose water >=20g/L, compound bacteria A >=50 × 109cfu/g；The compound bacteria A refers to beauveria bassiana spore >=50 ×109Cfu/g, Trichoderma spore >=50 × 109Cfu/g, saccharomycete spore >=50 × 109Cfu/g, photosynthetic bacteria spore >=50 × 109Cfu/g, muscardine spore >=50 × 109Cfu/g compound bacteria.
  2. 2. the method as described in claim 1, it is characterised in that further comprising the steps of：Before the sowing of fruits and vegetables seed, using exempting from The 1000-1500 times of dilution immersion fruits and vegetables seed 1-2 hours of epidemic disease vaccine.
  3. 3. method as claimed in claim 1 or 2, it is characterised in that main component includes in the soil-repairing agent：Biology is living Property polypeptide >=100g/L, agricultural amino acid original powder mass content >=2.0%, compound bacteria B >=100 × 109cfu/g；It is wherein described Compound bacteria B includes the flora of following strain：Bacillus globigii spores 2 × 109Cfu/g, actinospore >=40 × 109cfu/ G, saccharomycete spore >=40 × 109Cfu/g, nitrogen-fixing bacteria spore >=40 × 109Cfu/g, silicate bacteria spore >=40 × 109cfu/ G, Paecilomyces lilacinus spore >=40 × 109Cfu/g ,/gram of compound bacteria spore >=500,000,000 of bacillusmusilaginosiengineering, photosynthetic bacteria / gram of spore >=5,000,000,000, beauveria bassiana spore >=40 × 109Cfu/g, Trichoderma spore >=40 × 109Cfu/g, Bacillus cercus Spore >=4 × 109Cfu/g, gluey subtilis spore >=4 × 109Cfu/g, aspergillus oryzae spore >=4 × 109Cfu/g, huge bud Spore bacillus spores >=4 × 109cfu/g。
  4. 4. method as claimed in claim 3, it is characterised in that by weight, the agricultural amino acid original powder includes dried meat ammonia Sour 1-2 parts, glycine 1-2 parts, nitrogen propylhomoserin 1-2 parts, tryptophan 0.5-1 parts, cysteine 1-2 parts and alanine 0.5-1 parts.
  5. 5. method as claimed in claim 1 or 2, it is characterised in that the biological organic fertilizer is mainly by the plant of 30-50 parts by weight Thing stalk, turf 5-10 parts by weight, 5-10 parts by weight cakes powder, 5-10 parts by weight cake-shaped rape seed dregs cake, 5-10 parts by weight animal bone powder, 1-2 The calcium superphosphate of parts by weight, the humic acid of 2-5 parts by weight, the middle trace element of 0.1-1 parts by weight carry out aerobic compost after mixing, Through abundant fermentation maturity, the compound bacteria C acquisitions of 2-3 parts by weight are added；Following raw material is included in the compound bacteria C：Photosynthetic bacteria Spore >=40 × 109Cfu/g, saccharomycete spore >=40 × 109Cfu/g, cellulase >=1,000,000,000/g, protease >=1,000,000,000/g, shallow lake Powder enzyme >=40 × 109Cfu/g, pectase >=40 × 109Cfu/g, hemicellulase >=40 × 109Cfu/g, agricultural amino acid are former Powder mass fraction >=2.0%, spore phosphorus >=6 × 109Cfu/g and spore potassium >=6 × 109cfu/g。
  6. 6. method as claimed in claim 1 or 2, it is characterised in that in the step (5), immune vaccine sprayed every 15-20 days Once.
  7. 7. method as claimed in claim 1 or 2, it is characterised in that in the step (1), using the 300- of soil-repairing agent 400 times of dilution gardenfuls spray 1-2 progress rehabilitating soil.
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CN108703025A (en) * 2018-05-29 2018-10-26 海南农佳佳科技发展有限公司 A kind of ecology planting method promoting tomato mouthfeel
CN108718835A (en) * 2018-04-20 2018-11-02 申琳 A kind of method of environmentally friendly prevention and control plant disease
CN109906842A (en) * 2018-12-13 2019-06-21 哈尔滨森荞生物科技有限公司 Application of the super quick albumen complex enzyme formulation in prevention and treatment yellow twig
CN110214623A (en) * 2019-07-09 2019-09-10 广东茂益园林有限公司 A kind of Chinese photinia early stage nursery plantation technique
CN110218124A (en) * 2019-07-09 2019-09-10 中山市中茂环境建设有限公司 A kind of disease-resistant biological composition of Chinese photinia
CN111849490A (en) * 2020-07-21 2020-10-30 成都华宏生物科技有限公司 Composition for biologically preventing and treating pepper phytophthora blight and preparation method and application thereof
CN112075159A (en) * 2020-09-15 2020-12-15 慈溪市农业技术推广中心(慈溪市种子管理站) Saline-alkali-resistant high-yield and high-efficiency cultivation method for peanuts
CN113248304A (en) * 2021-05-14 2021-08-13 台沃科技集团股份有限公司 Multi-effect nutritional composition for preventing and treating actinidia chinensis root cancer and application thereof
CN115517097A (en) * 2022-09-15 2022-12-27 山东滨州国家农业科技园区管理服务中心(滨州黄河三角洲高效生态产业现代技术研究院) Device for preventing and treating 'short-shoot' winter jujube fruit shrinkage and using method
CN117004508A (en) * 2023-05-22 2023-11-07 湖北同光生物科技有限公司 Bacillus composite microbial inoculum and application thereof in citrus disease prevention and control
CN117223526A (en) * 2023-11-14 2023-12-15 山东省寿光蔬菜产业集团有限公司 Method for controlling occurrence of tomato late blight

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CN108718835A (en) * 2018-04-20 2018-11-02 申琳 A kind of method of environmentally friendly prevention and control plant disease
CN108703025A (en) * 2018-05-29 2018-10-26 海南农佳佳科技发展有限公司 A kind of ecology planting method promoting tomato mouthfeel
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CN110218124A (en) * 2019-07-09 2019-09-10 中山市中茂环境建设有限公司 A kind of disease-resistant biological composition of Chinese photinia
CN111849490B (en) * 2020-07-21 2022-01-25 成都华宏生物科技有限公司 Composition for biologically preventing and treating pepper phytophthora blight and preparation method and application thereof
CN111849490A (en) * 2020-07-21 2020-10-30 成都华宏生物科技有限公司 Composition for biologically preventing and treating pepper phytophthora blight and preparation method and application thereof
CN112075159A (en) * 2020-09-15 2020-12-15 慈溪市农业技术推广中心(慈溪市种子管理站) Saline-alkali-resistant high-yield and high-efficiency cultivation method for peanuts
CN113248304A (en) * 2021-05-14 2021-08-13 台沃科技集团股份有限公司 Multi-effect nutritional composition for preventing and treating actinidia chinensis root cancer and application thereof
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CN115517097B (en) * 2022-09-15 2023-11-10 河北农业大学 Device for preventing and controlling fruit shrinkage of winter jujubes with short branches and application method thereof
CN117004508A (en) * 2023-05-22 2023-11-07 湖北同光生物科技有限公司 Bacillus composite microbial inoculum and application thereof in citrus disease prevention and control
CN117004508B (en) * 2023-05-22 2024-02-09 湖北同光生物科技有限公司 Bacillus composite microbial inoculum and application thereof in citrus disease prevention and control
CN117223526A (en) * 2023-11-14 2023-12-15 山东省寿光蔬菜产业集团有限公司 Method for controlling occurrence of tomato late blight
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