CN105859338A - Functional microbial soil conditioner and preparation method thereof - Google Patents
Functional microbial soil conditioner and preparation method thereof Download PDFInfo
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- CN105859338A CN105859338A CN201610213577.XA CN201610213577A CN105859338A CN 105859338 A CN105859338 A CN 105859338A CN 201610213577 A CN201610213577 A CN 201610213577A CN 105859338 A CN105859338 A CN 105859338A
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- bacillus
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a functional microbial soil conditioner and a preparation method thereof. The functional microbial soil conditioner mainly comprises the following raw materials in parts by weight: 50-100 parts of mushroom dreg and 5-40 parts of microbial composite bacteria. The microbial composite bacteria are a mixture of Bacillus, saccharomycetes, pseudomonas fluorescens and Bacillus thuringiensis which are respectively independently cultured to the mature stable stage, and the total living bacteria count of the microbial composite bacteria is greater than or equal to 0.2 billion/g conditioner. The conditioner can change the organic content and humus content of soil, improve the soil structure, promote the formation of the soil aggregate and enhance the fertility of the soil.
Description
Technical field
The present invention relates to biological product technical field, particularly relate to a kind of functional microbial soil conditioner and preparation thereof
Method.
Background technology
Intensity of soil respiration is as one of biological activity of soil index, it is possible to react soil nutrient thing to a certain extent
Converting and deliverability, the change of its breathing rate and change direction have also reacted the ecosystem sensitivity to coercing and response
Pattern, is an important indicator of environmental safety assessment, and when soil is polluted by external contaminant, microorganism is in order to remain raw
Deposit and may need more energy, and make the metabolic activity of soil microorganism that response in various degree to occur.
Bacillus subtilis is widely used in bio-feritlizer.Can be at crop rhizosphere or body when acting on crop or soil
Decided at the higher level but not officially announced grow, and play specific fertilizer effect.At present, microbial manure, at culture fertility, improves chemical fertilizer utilization ratio, suppresses crops
To nitrate nitrogen, heavy metal, the absorption of pesticide, purify and rehabilitating soil, reduce corps diseases and occur, promote agricultural crop straw and
The utilization of becoming thoroughly decomposed of municipal refuse. protection environment.And the raising aspect such as crop products quality and food safety shows not
Alternative effect.In soil, the accumulation of salinity causes Soil structure viscid, and aeration is poor, and unit weight is high, and soil temperature rises, aerobic
Microbial activities is poor, and nutrients release is slow, and infiltration coefficient is low, and capillarity is strong, causes topsoil salination to be further exacerbated by,
Cause that soil is cold, hard, plate phenomenon.
Bacillus megaterium can be used to produce the solid potash fertilizer of phosphorus decomposing, and bacillus megaterium has in soil of well degrading and has
Effect of machine phosphorus, is the conventional strain producing biological organic fertilizer, is also the conventional strain making water body inorganic agent.
Bacillus licheniformis adjustable dysbacteriosis reaches therapeutic purposes, and body can be promoted to produce antibacterial substance, kill
Go out pathogenic bacterium.Can produce resistant activity material, and the biology with uniqueness takes oxygen mechanism of action by force, the growth that can suppress pathogenic bacterium is numerous
Grow.
Saccharomycodes fungus, is distributed widely in nature, especially more on Fructus Vitis viniferae and other various fruits and vegetable.It is
Important ferment, can decompose carbohydrate and produce ethanol and carbon dioxide etc., also have for fermenting.
Pseudomonas fluorescens is distributed widely in nature, in soil, water, plant and animal activity environment.This bacterium is biochemical
Ability is enlivened, and degradable many artificial-synthetic compounds are commonly used for environmental conservation.Or a kind of important plant rhizosphere growth-promoting
Antibacterial, is one of bacterial species that in known plants beneficial rhizosphere microorganisms, population quantity is more.This bacterium nutritional need is the simplest
Single, it is possible to utilize major part nutrition in root exudates surely to grow at plant rhizosphere rapidly.Some of them bacterial strain has promotion plant
Growth and the effect of controlling disease, thus it is possibly used for the Biological control of plant disease.
Bacillus thuringiensis microorganism belonging to genus insecticide, can be used for the insect in farming and forestry and underground, to Lepidoptera evil
Worm and pine moth have good prevention effect, and are harmless boilogical insecticide, are mainly used in preventing and treating cereal crops, cotton, Fructus Mali pumilae
Deng the lepidoptera pest of crop, belong to aerobic Bacillus cercus group.
Summary of the invention
The technical problem to be solved is to provide one and can change soil organic matter content and humus content, change
Kind Soil structure, the functional microbial soil conditioner increase soil fertility.
For solving above-mentioned technical problem, the technical scheme is that
Functional microbial soil conditioner, mainly includes following parts by weight raw material:
Mushroom slag 50～100 parts；
Microbial composite bacteria 5～40 parts；
Wherein, microbial composite bacteria is that bacillus cereus, yeast, pseudomonas fluorescens and Bacillus thuringiensis are individually trained
Support the mixture to mature and stable after date, total living bacteria count of described microbial composite bacteria >=2.0 hundred million/gram modifying agents.
Wherein, described bacillus cereus is bacillus subtilis, bacillus megaterium, Bacillus licheniformis, colloid spore bar
One or more in bacterium, lactic acid bacillus.
As preferably, in described microbial composite bacteria, the parts by weight of each strain are: bacillus subtilis 1～10 parts；Huge
Bacterium anthracoides 0.5～10 parts；Bacillus licheniformis 0.5～10 parts；Yeast 0.5～10 parts；Pseudomonas fluorescens 0.5～10
Part；Bacillus thuringiensis 0.5～10 parts.
The method that present invention also offers preparation above-mentioned functions microbe soil conditioner: by bacillus subtilis, huge
Bacterium anthracoides, Bacillus licheniformis, yeast, pseudomonas fluorescens and Bacillus thuringiensis single culture, the most each strain is ripe
Change to be combined after entering stable phase and make it into steady statue, again with the mushroom slag after sterilizing, each strain is adsorbed also
Cultivate under aseptic conditions to ripening.
As preferably, described bacillus subtilis condition of culture is: peptone 5-10 gram, beef extract 1-3 gram, chlorination
2-5 gram of sodium, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation and arrive stable phase in 25～35 days.
As preferably, described bacillus megaterium condition of culture is: peptone 5-10 gram, beef extract 1-3 gram, chlorination
2-5 gram of sodium, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation and arrive stable phase in 25～35 days.
As preferably, described Bacillus licheniformis condition of culture is: peptone 5-10 gram, beef extract 1-3 gram, chlorination
2-5 gram of sodium, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation and arrive stable phase in 25～35 days.
As preferably, described yeast condition of culture is: 12Brix. beerwort 1L, protein 15-20 grams, natural pH, cultivates
Activate 25～35 days to stable phase.
As preferably, described pseudomonas fluorescens condition of culture is: peptone 5-10 gram, beef extract 1-3 gram, chlorination
2-5 gram of sodium, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation and arrive stable phase in 25～35 days.
As preferably, described Bacillus thuringiensis condition of culture is: peptone 5-10 gram, beef extract 1-3 gram, sodium chloride
2-5 gram, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation and arrive stable phase in 25～35 days.
Compared with prior art, the method have the advantages that
(1) characteristic utilizing mentioned microorganism is carried out individual plant in incubation and is cultivated, and the mature and stable phase is combined to be made
It enters steady statue, again with the mushroom slag after sterilizing, strain is adsorbed cultivation under aseptic condition and makes it ripe in about 30 days
Change, soil organic matter content, humus content can be changed, improve Soil structure, promote the formation of soil aggregate, improve soil
The fertility of earth.
(2) in the fruit gardens such as mandarin orange, Fructus Mali pumilae, pears, Fructus Vitis viniferae by dreg deep placement after bury, improvement orchard soil can be played,
Improve the effect of fruit quality, and fertilizer efficiency is lasting, economical and practical.Modifying agent once enters and runs into suitable nutrition among soil
Become branch expand rapidly and give full play to the effect of each bacterial strain, repaired for some pathological changes in soil.
(3) simultaneously also can be as vegetable base manure.Carrying out Plantula Brassicae chinensis plot experiment after utilizing mushroom slag fermentation, result shows, mushroom
The fertilizer that slag is made contributes to improving Plantula Brassicae chinensis biological character, the property such as the blade of Plantula Brassicae chinensis, petiole length and width of blade
Shape is better than conventional treatment, and yield of pakchoi volume increase 11.2%, and chlorophyll content improves 9.3%, and soluble sugar content improves
3.9%, nutritional quality is improved.
(4) the mushroom slag after becoming thoroughly decomposed sieves, as the improved materials of turfgrass bed.Be respectively adopted covering, mix, mix+
Cover 3 kinds of modes, soil is improved.3 kinds of mode of ameliorating are all to loosing soil performance, soil moisture content, soil permeability
Obvious improvement result can be shown.With covering treatment best results, there is " insulation effect " when low ambient temperature, at high ambient temperature
Time have " effects of reduced temperature ", significantly increase the tiller density of turfgrass, promote the growth of turfgrass, and the thickest effect of cover layer
The best.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is expanded on further.
Embodiment 1
Cultivate bacillus subtilis, bacillus megaterium, Bacillus licheniformis, yeast, pseudomonas fluorescens, Soviet Union respectively
Cloud gold bacillus, to mature and stable after date, takes mushroom slag 80 parts by weight；Bacillus subtilis 10 parts；Bacillus megaterium 2 parts；
Bacillus licheniformis 2 parts；5 parts of yeast；Pseudomonas fluorescens 5 parts；Bacillus thuringiensis 5 parts；It is combined to make it into and is stablized
State, utilizes the mushroom slag after sterilizing to be adsorbed each strain and cultivation about 30 days, to ripening, prepares merit under aseptic conditions
Can property microbe soil conditioner.
Above-mentioned soil conditioner can change soil organic matter content, humus content, improves Soil structure, promotes soil group
The formation of kernel structure, improves the fertility of soil.
Embodiment 2
Cultivate bacillus subtilis, bacillus megaterium, Bacillus licheniformis, yeast, pseudomonas fluorescens, Soviet Union respectively
Cloud gold bacillus, to mature and stable after date, takes mushroom slag 100 parts by weight；Bacillus subtilis 5 parts；Bacillus megaterium 2 parts；
Bacillus licheniformis 2 parts；10 parts of yeast；Pseudomonas fluorescens 6 parts；Bacillus thuringiensis 5 parts；It is combined to make it into and is stablized
State, utilizes the mushroom slag after sterilizing to be adsorbed each strain and cultivation about 30 days, to ripening, prepares merit under aseptic conditions
Can property microbe soil conditioner.
Utilizing above-mentioned soil conditioner to carry out Plantula Brassicae chinensis plot experiment, result shows, the fertilizer that mushroom slag is made contributes to
Improving Plantula Brassicae chinensis biological character, the character such as the blade of Plantula Brassicae chinensis, petiole length and width of blade is better than conventional treatment, and little Bai
Dish yield volume increase 11.2%, chlorophyll content improves 9.3%, and soluble sugar content improves 3.9%, and nutritional quality is improved.
Embodiment 3
Preparing soil conditioner according to the method for embodiment 1, sieve the improved materials as turfgrass bed.It is respectively adopted
Cover, mix, mix+cover 3 kinds of modes, soil is improved.Loosing soil performance, soil are all contained by 3 kinds of mode of ameliorating
The water yield, soil moisture monitoring show obvious improvement result.With covering treatment best results, there is " insulation when low ambient temperature
Effect ", have " effects of reduced temperature " when high ambient temperature, significantly increase the tiller density of turfgrass, promote the growth of turfgrass,
And the thickest effect of cover layer is the best.
The foregoing is only the schematic detailed description of the invention of the present invention, be not limited to the scope of the present invention.Any
Those skilled in the art, equivalent variations done on the premise of without departing from the design of the present invention and principle and amendment, all
The scope of protection of the invention should be belonged to.
Claims (10)
1. a functional microbial soil conditioner, it is characterised in that mainly include following parts by weight raw material:
Mushroom slag 50～100 parts；
Microbial composite bacteria 5～40 parts；
Wherein, microbial composite bacteria be bacillus cereus, yeast, pseudomonas fluorescens and Bacillus thuringiensis individually cultivate to
The mixture of mature and stable after date, total living bacteria count of described microbial composite bacteria >=2.0 hundred million/gram modifying agents.
2. functional microbial soil conditioner as claimed in claim 1, it is characterised in that: described bacillus cereus is hay bud
One or more in spore bacillus, bacillus megaterium, Bacillus licheniformis, colloid bacillus cereus, lactic acid bacillus.
3. functional microbial soil conditioner as claimed in claim 2, it is characterised in that: each in described microbial composite bacteria
The parts by weight of strain are: bacillus subtilis 1～10 parts；Bacillus megaterium 0.5～10 parts；Bacillus licheniformis 0.5～
10 parts；Yeast 0.5～10 parts；Pseudomonas fluorescens 0.5～10 parts；Bacillus thuringiensis 0.5～10 parts.
4. the method preparing functional microbial soil conditioner as claimed in claim 3, it is characterised in that: by hay spore
Bacillus, bacillus megaterium, Bacillus licheniformis, yeast, pseudomonas fluorescens and Bacillus thuringiensis single culture, wait until each
Strain ripening enters to be combined after stable phase and makes it into steady statue, again with the mushroom slag after sterilizing to each strain in addition
Absorption is also cultivated under aseptic conditions to ripening.
5. preparation method as claimed in claim 4, it is characterised in that: described bacillus subtilis condition of culture is: peptone
5-10 gram, beef extract 1-3 gram, 2-5 gram of sodium chloride, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation 25
～arrive stable phase in 35 days.
6. preparation method as claimed in claim 4, it is characterised in that: described bacillus megaterium condition of culture is: peptone
5-10 gram, beef extract 1-3 gram, 2-5 gram of sodium chloride, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation 25
～arrive stable phase in 35 days.
7. preparation method as claimed in claim 4, it is characterised in that: described Bacillus licheniformis condition of culture is: peptone
5-10 gram, beef extract 1-3 gram, 2-5 gram of sodium chloride, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation 25
～arrive stable phase in 35 days.
8. preparation method as claimed in claim 4, it is characterised in that: described yeast condition of culture is: 12Brix. beerwort
1L, protein 15-20 grams, natural pH, cultivates activation and arrives stable phase in 25～35 days.
9. preparation method as claimed in claim 4, it is characterised in that: described pseudomonas fluorescens condition of culture is: peptone
5-10 gram, beef extract 1-3 gram, 2-5 gram of sodium chloride, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation 25
～arrive stable phase in 35 days.
10. preparation method as claimed in claim 4, it is characterised in that: described Bacillus thuringiensis condition of culture is: peptone 5-
10 grams, beef extract 1-3 gram, 2-5 gram of sodium chloride, protein 10-20 grams, distilled water 0.5-1L, pH7.0, cultivate activation 25～
35 days arrive stable phase.
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| CN201610213577.XA CN105859338A (en) | 2016-04-07 | 2016-04-07 | Functional microbial soil conditioner and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635042A (en) * | 2016-12-27 | 2017-05-10 | 海南绿藻世界生物科技有限公司 | Chlorella soil conditioner, and preparation method and application thereof |
| CN107915510A (en) * | 2016-10-10 | 2018-04-17 | 云南福保农业科技开发有限公司 | A kind of Pleurotus sp2 slag biological fertilizer and its preparation and application |
| CN108114976A (en) * | 2017-12-19 | 2018-06-05 | 轻工业环境保护研究所 | A kind of method of repairing polluted soil |
| CN108994065A (en) * | 2018-07-05 | 2018-12-14 | 上海黑维环保发展有限公司 | A kind of multiple-effect renovation agent preparation of contaminated soil and its restorative procedure |
| CN109054843A (en) * | 2018-08-03 | 2018-12-21 | 北京农业生物技术研究中心 | A kind of soil conditioner and preparation method and its application as pesticide synergistic agent |
| CN111499432A (en) * | 2020-04-13 | 2020-08-07 | 山东省农业科学院农业资源与环境研究所 | Microbial remediation microbial inoculum for saline-alkali soil as well as preparation method and application of microbial remediation microbial inoculum |
| CN113265254A (en) * | 2021-07-01 | 2021-08-17 | 山西大地生态环境技术研究院有限公司 | Microcapsule type soil conditioner containing microorganisms and preparation method and application thereof |
| CN113604403A (en) * | 2021-08-31 | 2021-11-05 | 易之泰生物科技（龙岩）有限公司 | Multi-strain combined microbial inoculum for improving soil and application thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107915510A (en) * | 2016-10-10 | 2018-04-17 | 云南福保农业科技开发有限公司 | A kind of Pleurotus sp2 slag biological fertilizer and its preparation and application |
| CN106635042A (en) * | 2016-12-27 | 2017-05-10 | 海南绿藻世界生物科技有限公司 | Chlorella soil conditioner, and preparation method and application thereof |
| CN108114976A (en) * | 2017-12-19 | 2018-06-05 | 轻工业环境保护研究所 | A kind of method of repairing polluted soil |
| CN108114976B (en) * | 2017-12-19 | 2021-10-15 | 轻工业环境保护研究所 | Method for restoring polluted soil |
| CN108994065A (en) * | 2018-07-05 | 2018-12-14 | 上海黑维环保发展有限公司 | A kind of multiple-effect renovation agent preparation of contaminated soil and its restorative procedure |
| CN108994065B (en) * | 2018-07-05 | 2020-09-08 | 上海黑维环保发展有限公司 | Preparation of multi-effect remediation agent for contaminated soil and remediation method thereof |
| CN109054843A (en) * | 2018-08-03 | 2018-12-21 | 北京农业生物技术研究中心 | A kind of soil conditioner and preparation method and its application as pesticide synergistic agent |
| CN111499432A (en) * | 2020-04-13 | 2020-08-07 | 山东省农业科学院农业资源与环境研究所 | Microbial remediation microbial inoculum for saline-alkali soil as well as preparation method and application of microbial remediation microbial inoculum |
| CN111499432B (en) * | 2020-04-13 | 2022-07-22 | 山东省农业科学院农业资源与环境研究所 | Microbial remediation microbial inoculum for saline-alkali soil as well as preparation method and application of microbial remediation microbial inoculum |
| CN113265254A (en) * | 2021-07-01 | 2021-08-17 | 山西大地生态环境技术研究院有限公司 | Microcapsule type soil conditioner containing microorganisms and preparation method and application thereof |
| CN113604403A (en) * | 2021-08-31 | 2021-11-05 | 易之泰生物科技（龙岩）有限公司 | Multi-strain combined microbial inoculum for improving soil and application thereof |
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