CN107365725A - A kind of organic matter decomposing inoculant and preparation method thereof - Google Patents
A kind of organic matter decomposing inoculant and preparation method thereof Download PDFInfo
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- CN107365725A CN107365725A CN201710648274.5A CN201710648274A CN107365725A CN 107365725 A CN107365725 A CN 107365725A CN 201710648274 A CN201710648274 A CN 201710648274A CN 107365725 A CN107365725 A CN 107365725A
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- fermentation
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- aspergillus oryzae
- seed liquor
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- 238000002360 preparation method Methods 0.000 title claims abstract description 100
- 239000002054 inoculum Substances 0.000 title claims abstract description 85
- 239000005416 organic matter Substances 0.000 title claims abstract description 51
- 238000000855 fermentation Methods 0.000 claims abstract description 208
- 230000004151 fermentation Effects 0.000 claims abstract description 208
- 239000007787 solid Substances 0.000 claims abstract description 76
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 62
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 62
- 241000894006 Bacteria Species 0.000 claims abstract description 57
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 49
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 44
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 44
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 39
- 241000186046 Actinomyces Species 0.000 claims abstract description 29
- 241000194017 Streptococcus Species 0.000 claims abstract description 25
- 239000004310 lactic acid Substances 0.000 claims abstract description 21
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 21
- 239000000463 material Substances 0.000 claims abstract description 19
- 241000195940 Bryophyta Species 0.000 claims abstract description 13
- 239000012530 fluid Substances 0.000 claims description 74
- 239000002609 medium Substances 0.000 claims description 73
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 70
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 56
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 42
- 235000015097 nutrients Nutrition 0.000 claims description 36
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 35
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 35
- 239000001963 growth medium Substances 0.000 claims description 34
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 30
- 239000001888 Peptone Substances 0.000 claims description 30
- 108010080698 Peptones Proteins 0.000 claims description 30
- 239000008103 glucose Substances 0.000 claims description 30
- 235000019319 peptone Nutrition 0.000 claims description 30
- 238000007790 scraping Methods 0.000 claims description 25
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 24
- 239000011780 sodium chloride Substances 0.000 claims description 21
- 229940041514 candida albicans extract Drugs 0.000 claims description 20
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 20
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 20
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 20
- 239000012138 yeast extract Substances 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 19
- 210000003608 fece Anatomy 0.000 claims description 18
- 239000010871 livestock manure Substances 0.000 claims description 15
- 241000287828 Gallus gallus Species 0.000 claims description 14
- 240000008042 Zea mays Species 0.000 claims description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 13
- 235000005822 corn Nutrition 0.000 claims description 13
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 10
- 239000004202 carbamide Substances 0.000 claims description 10
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 10
- 229920000053 polysorbate 80 Polymers 0.000 claims description 10
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 10
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 9
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 235000015278 beef Nutrition 0.000 claims description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 230000000295 complement effect Effects 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 229940099596 manganese sulfate Drugs 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- 235000013336 milk Nutrition 0.000 claims description 5
- 239000008267 milk Substances 0.000 claims description 5
- 210000004080 milk Anatomy 0.000 claims description 5
- 239000004323 potassium nitrate Substances 0.000 claims description 5
- 235000010333 potassium nitrate Nutrition 0.000 claims description 5
- 239000001632 sodium acetate Substances 0.000 claims description 5
- 235000017281 sodium acetate Nutrition 0.000 claims description 5
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 5
- 239000001393 triammonium citrate Substances 0.000 claims description 5
- 235000011046 triammonium citrate Nutrition 0.000 claims description 5
- 239000012137 tryptone Substances 0.000 claims description 5
- 241000186361 Actinobacteria <class> Species 0.000 claims description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 2
- 239000005864 Sulphur Substances 0.000 claims description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- 240000003768 Solanum lycopersicum Species 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 claims 1
- 239000011790 ferrous sulphate Substances 0.000 claims 1
- 235000003891 ferrous sulphate Nutrition 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- 229910052742 iron Inorganic materials 0.000 claims 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 13
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 7
- 239000003795 chemical substances by application Substances 0.000 abstract description 7
- 239000011574 phosphorus Substances 0.000 abstract description 7
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 6
- 239000011591 potassium Substances 0.000 abstract description 6
- 229910052700 potassium Inorganic materials 0.000 abstract description 6
- 239000011368 organic material Substances 0.000 abstract description 5
- 238000010792 warming Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 230000008635 plant growth Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 31
- HNBDQABBWNOTRU-UHFFFAOYSA-N thalline Chemical compound C1=CC=[Tl]C=C1 HNBDQABBWNOTRU-UHFFFAOYSA-N 0.000 description 21
- 238000009630 liquid culture Methods 0.000 description 12
- 241000209094 Oryza Species 0.000 description 9
- 235000007164 Oryza sativa Nutrition 0.000 description 9
- 239000010903 husk Substances 0.000 description 9
- 235000009566 rice Nutrition 0.000 description 9
- 241000209140 Triticum Species 0.000 description 8
- 235000021307 Triticum Nutrition 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000003337 fertilizer Substances 0.000 description 6
- 238000009264 composting Methods 0.000 description 5
- 230000035784 germination Effects 0.000 description 5
- 244000144977 poultry Species 0.000 description 5
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- 241000227653 Lycopersicon Species 0.000 description 4
- 239000000460 chlorine Substances 0.000 description 4
- 229910052801 chlorine Inorganic materials 0.000 description 4
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- GMKMEZVLHJARHF-UHFFFAOYSA-N (2R,6R)-form-2.6-Diaminoheptanedioic acid Natural products OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- GMKMEZVLHJARHF-SYDPRGILSA-N meso-2,6-diaminopimelic acid Chemical compound [O-]C(=O)[C@@H]([NH3+])CCC[C@@H]([NH3+])C([O-])=O GMKMEZVLHJARHF-SYDPRGILSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of organic matter decomposing inoculant and preparation method thereof, decomposing agent includes bacillus subtilis, actinomyces, lactic acid bacteria, aspergillus oryzae and saccharomycete；Further, organic matter decomposing inoculant includes 5 10 parts of bacillus subtilis fermentation liquor, 40 45 parts of unwrapping wire bacteria solid fermentation thing, 5 10 parts of streptococcus acidi lactici fermented solution, 30 40 parts of Aspergillus oryzae solid culture, 5 10 parts of saccharomycetes to make fermentation liquid, 0 10 parts of rotten mosses ash.Preparation method：Bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation thing, streptococcus acidi lactici fermented solution, Aspergillus oryzae solid culture, saccharomycetes to make fermentation liquid and rotten mosses ash are mixed in proportion and produced.The organic matter decomposing inoculant of the present invention can make organic materials be warming up to more than 60 degree at 23 days, and continue 57 days at such a temperature；The present invention can discharge the slightly solubility potassium in material and phosphorus to accelerate plant growth, improved soil environment；Dosage of the present invention is few, and effect is good.
Description
Technical field
The present invention relates to a kind of organic matter decomposing inoculant and preparation method thereof, belong to technical field of soil fertilizer.
Background technology
Composting process is the process to stable humus biochemical conversion using microorganism promotion biodegradable organic,
Compost is divided into 4 stage heatings, high temperature, cooling and decomposed.With the development of livestock and poultry breeding industry, feces of livestock and poultry causes sternly to environment
The pollution of weight；Poultry manure is as the preferable organic fertilizer of fertilizer efficiency, but the wherein more presence in the form of organic compound of nutrient, difficult
Utilized with being absorbed by crops.Meanwhile field crops stalk not only wastes resource and pollution often through burning disposal, burning
Environment.
Bacillus subtilis is one kind of bacillus, is aerobic, bacillus, the bacterium is as plant disease biological and ecological methods to prevent plant disease, pests, and erosion
One of bacterium, there is stronger prophylaxis effect, in extreme conditions, can also induce and produce the very strong gemma of resistance, should
Bacterium is readily produced, and carries out formulation, is easy to survive again, colonizes and breed.
Actinomyces thalline is unicellular, is made up of mostly the flourishing mycelia of branch, simplest for shaft-like or tool original bacteria
Silk.Hyphal diameter is similar with bacillus, about 1 micron.Also born of the same parents specific to prokaryotes are contained in cell membrane chemical composition
Teichaic acid and diaminopimelic acid, without chitin or cellulose；One of its most prominent characteristic is to produce that substantial amounts of, species is numerous
More antibiotic.
Lactic acid bacteria refers to a kind of general name without gemma, gram-positive bacterium of the fermenting carbohydrate primary product for lactic acid；
Lactic acid bacteria can pass through the material such as organic acid, special enzyme system, sour rhzomorph caused by fermentation.
Aspergillus oryzae can intensive decomposition protein, cellulose, hemicellulose, lignin etc., and by thermophilic, thermoduric bacteria,
Fungi, yeast strain and correlation down enzyme are combined, and its living bacteria count content is high, and degradation capability is strong, while can reach
To heating, deodorization, eliminate pest and disease damage, weed seed and the effect for improving nutrient.Under appropriate conditions, can be rapidly by windrow
Carbon, nitrogen, phosphorus, potassium, sulphur etc. decompose mineralising, form simple organic.
Saccharomycete is some unicellular fungis, not the unit of phylogeny classification.A kind of invisible small list
Cell microorganism, sugar can be fermented into alcohol and carbon dioxide, be distributed in whole nature, be that a kind of typical amphimicrobian is micro-
It biology, can be survived under aerobic and oxygen free condition, be a kind of natural fermented dose.
The content of the invention
The invention provides a kind of organic matter decomposing inoculant and preparation method thereof, the present invention can make material (feces of livestock and poultry,
Mushroom residue, crop material, bean dregs etc.) it is brought rapidly up, more than 60 degree are warming up within 2-3 days, quick composting, 10 days or so are decomposed complete
Entirely.Technical scheme is as follows：
A kind of organic matter decomposing inoculant, including following components：
Bacillus subtilis, actinomyces, lactic acid bacteria, aspergillus oryzae and saccharomycete.
Further, organic matter decomposing inoculant of the present invention, the component of following parts by weight is included：
Bacillus subtilis fermentation liquor 5-10 parts, unwrapping wire bacteria solid fermentation thing 40-45 parts, streptococcus acidi lactici fermented solution 5-10 parts,
Aspergillus oryzae solid culture 30-40 parts, saccharomycetes to make fermentation liquid 5-10 parts, rotten mosses ash 0-10 parts.
Further, organic matter decomposing inoculant of the present invention, the component of following parts by weight is included：
7 parts of bacillus subtilis fermentation liquor, 42 parts of unwrapping wire bacteria solid fermentation thing, 9 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid
34 parts of fermentate, 6 parts of saccharomycetes to make fermentation liquid, 4 parts of rotten mosses ash.
In the present invention, the preparation method of organic matter decomposing inoculant is as follows：
Firstth, the preparation of bacillus subtilis fermentation liquor：
(1) preparation of seed liquor：The thalline that scraping one connects collarium bacillus subtilis from slant medium is inoculated into liquid
In body culture medium, shaking table culture 12h, rotating speed 200r/min, temperature are 30 DEG C, obtain the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L)：Tryptone 10, yeast extract 5, NaCl 10, water complements to
1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is that 100-120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h,
Fermentation time is 18-20h, produces bacillus subtilis fermentation liquor；Preferably, rotating speed is 110 turns/min, fermentation time 19h.
Fermentation medium (g/L)：Corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9th, ammonium sulfate 1, K2HPO4
0.3rd, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
Secondth, the preparation of unwrapping wire bacteria solid fermentation thing：
(1) preparation of seed liquor：The thalline that scraping one connects collarium actinomyces from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L)：Potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5,
Ferrous sulfate heptahydrate 0.01, water complements to 1L；
(2) preparation of zymotic fluid：
By step (1) obtain seed liquor be inoculated into 10% (v/v) inoculum concentration in fermentation tank, rotating speed be 120 turns/
Min, temperature are 28 DEG C, 200L fermentation tanks, throughput 6m3Fermented, fermentation time 30-32h, produced under conditions of/h
Actinomycetes fermentation liquor；Preferably, fermentation time 31h.
Actinomyces liquid fermentation medium (g/L)：Glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2,
Dipotassium hydrogen phosphate 0.4, epsom salt 0.05, water complements to 1L.
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is inoculated into 10% inoculum concentration (m/m)
In unwrapping wire bacteria solid fermentation culture medium, mix；2. heap processed：Length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Bar pile；3. fermentation management：When temperature of charge no longer improves after heating up, turning continues culture 3-5 days, produces actinomyces and consolidates
Body fermentate；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent)：Chicken manure (dry weight) 50%, mushroom residue 50%, mix.
3rd, the preparation of streptococcus acidi lactici fermented solution：
(1) preparation of seed liquor：The thalline that scraping one connects collarium lactic acid bacteria from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L)：Peptone 10.0, powdered beef 5.0, dusty yeast 4.0, glucose 20.0, tween
80 1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to
1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is that 100-120 turns/min in rotating speed, temperature is 28 DEG C, 200L fermentation tanks, throughput 6m3Under conditions of/h, pH are 6-6.5
Fermented, fermentation time 22-24h, produce streptococcus acidi lactici fermented solution；Preferably, rotating speed is 110 turns/min, pH 6.2, is sent out
The ferment time is 23h.
Lactobacillus-fermented culture medium (g/L)：Peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato
Powder 0.25, Tween 80 0.1 are leached, water complements to 1L.
4th, the preparation of Aspergillus oryzae solid culture：
(1) preparation of seed liquor：The preparation of seed liquor：The thalline that scraping one connects collarium aspergillus oryzae from slant medium connects
Kind is into fluid nutrient medium, shaking table culture 12h, rotating speed 220r/min, and temperature is 28 DEG C, obtains the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L)：Urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05th, potassium dihydrogen phosphate 0.18, water complement to 1L.
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, during fermentation
Between be 28-30h, produce aspergillus oryzae zymotic fluid；Preferably, fermentation time 29h.
Aspergillus oryzae fermentation medium (g/L)：Urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride
0.5, ferric sulfate 0.5. water supplies 1L；
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is connect with 10-15% inoculum concentration (m/m)
Kind mixes into aspergillus oryzae solid fermentation culture medium；2. heap processed：Length 10m, wide 2-4m, height 30- is made in the material of mixing
40cm bar pile；3. fermentation management：When temperature of charge after heating up is not improving, turning continues culture 3-5 days, produces meter Qu
Mould solid fermentation thing；
Aspergillus oryzae solid fermentation culture medium is mushroom residue.
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor：The thalline that scraping one connects collarium saccharomycete from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L)：Yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, during fermentation
Between be 12-14h, produce saccharomycetes to make fermentation liquid；Preferably, fermentation time 13h.
Saccharomycetes to make fermentation culture medium (g/L)：Peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, chlorine
Change sodium 5, add water to 1L, pH 7.0-7.2；
6th, bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation thing, streptococcus acidi lactici fermented solution, aspergillus oryzae solid are sent out
Ferment thing, saccharomycetes to make fermentation liquid and rotten mosses ash, which mix in proportion, to be produced.
The present invention has advantages below compared with prior art：
(1) organic matter decomposing inoculant of the invention can make organic materials be warming up to more than 60 degree at 2-3 days, and in the temperature
Degree is lower to continue 5-7 days；
(2) present invention can discharge the slightly solubility potassium in material and phosphorus to accelerate plant growth, while the present invention
Addition has antagonistic strain to suppress the growth of pathogenic bacteria in soil, improved soil environment；
(3) dosage of the present invention is few, can quickly make organic material composting；
(4) present invention can not only be carried out decomposed to stalk, feces of livestock and poultry can also be carried out decomposed；
(5) straw-returning applies organic matter decomposing inoculant of the invention simultaneously can improve yield, and improve soil knot
Structure.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and
It is apparent.But embodiment is only exemplary, does not form any restrictions to the scope of the present invention.Those skilled in the art should
It should be appreciated that the details and form of technical solution of the present invention can be repaiied without departing from the spirit and scope of the invention
Change or replace, but these modifications and replacement are each fallen within protection scope of the present invention.
Mushroom and reagent used are commercially available in the present invention.
1 a kind of organic matter decomposing inoculant of embodiment and preparation method thereof
Organic matter decomposing inoculant includes the component of following parts by weight：
7 parts of bacillus subtilis fermentation liquor, 42 parts of unwrapping wire bacteria solid fermentation thing, 9 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid
34 parts of fermentate, 6 parts of saccharomycetes to make fermentation liquid, 4 parts of rotten mosses ash；
The preparation method of above-mentioned organic matter decomposing inoculant is as follows：
Firstth, the preparation of bacillus subtilis fermentation liquor：
(1) preparation of seed liquor：The thalline that scraping one connects collarium bacillus subtilis from slant medium is inoculated into liquid
In body culture medium, shaking table culture 12h, rotating speed 200r/min, temperature are 30 DEG C, obtain the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L)：Tryptone 10, yeast extract 5, NaCl 10, water complements to
1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 110 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented, fermented under conditions of/h
Time is 19h, produces bacillus subtilis fermentation liquor；
Fermentation medium (g/L)：Corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9th, ammonium sulfate 1, K2HPO4
0.3rd, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
Secondth, the preparation of unwrapping wire bacteria solid fermentation thing：
(1) preparation of seed liquor：The thalline that scraping one connects collarium actinomyces from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L)：Potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5,
Ferrous sulfate heptahydrate 0.01, water complements to 1L；
(2) preparation of zymotic fluid：
By step (1) obtain seed liquor be inoculated into 10% (v/v) inoculum concentration in fermentation tank, rotating speed be 120 turns/
Min, temperature are 28 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, fermentation time 31h, produce and put
Line fermented liquid；
Actinomyces liquid fermentation medium (g/L)：Glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2,
Dipotassium hydrogen phosphate 0.4, epsom salt 0.05, water complements to 1L.
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is inoculated into 10% inoculum concentration (m/m)
In unwrapping wire bacteria solid fermentation culture medium, mix；2. heap processed：Length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Bar pile；3. fermentation management：When temperature of charge no longer improves after heating up, turning continues culture 4 days, produces actinomyces solid
Fermentate；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent)：Chicken manure (dry weight) 50%, mushroom residue 50%, mix.
3rd, the preparation of streptococcus acidi lactici fermented solution：
(1) preparation of seed liquor：The thalline that scraping one connects collarium lactic acid bacteria from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L)：Peptone 10.0, powdered beef 5.0, dusty yeast 4.0, glucose 20.0, tween
80 1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to
1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 110 turns/min in rotating speed, temperature is 28 DEG C, 200L fermentation tanks, throughput 6m3/ h, pH are sent out under conditions of being 6.2
Ferment, fermentation time 23h, produces streptococcus acidi lactici fermented solution；
Lactobacillus-fermented culture medium (g/L)：Peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato
Powder 0.25, Tween 80 0.1 are leached, water complements to 1L.
4th, the preparation of Aspergillus oryzae solid culture：
(1) preparation of seed liquor：The preparation of seed liquor：The thalline that scraping one connects collarium aspergillus oryzae from slant medium connects
Kind is into fluid nutrient medium, shaking table culture 12h, rotating speed 220r/min, and temperature is 28 DEG C, obtains the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L)：Urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05th, potassium dihydrogen phosphate 0.18, water complement to 1L.
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, during fermentation
Between be 29h, produce aspergillus oryzae zymotic fluid；
Aspergillus oryzae fermentation medium (g/L)：Urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride
0.5, ferric sulfate 0.5. water supplies 1L；
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is connect with 10-15% inoculum concentration (m/m)
Kind mixes into aspergillus oryzae solid fermentation culture medium；2. heap processed：Length 10m, wide 2-4m, height 30- is made in the material of mixing
40cm bar pile；3. fermentation management：When temperature of charge after heating up is not improving, turning continues culture 4 days, produces aspergillus oryzae
Solid fermentation thing；
Aspergillus oryzae solid fermentation culture medium is mushroom residue.
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor：The thalline that scraping one connects collarium saccharomycete from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L)：Yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, during fermentation
Between be 13h, produce saccharomycetes to make fermentation liquid；
Saccharomycetes to make fermentation culture medium (g/L)：Peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, chlorine
Change sodium 5, add water to 1L, pH 7.0；
6th, bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation thing, streptococcus acidi lactici fermented solution, aspergillus oryzae solid are sent out
Ferment thing, saccharomycetes to make fermentation liquid and rotten mosses ash, which mix in proportion, to be produced.
2 a kind of organic matter decomposing inoculant of embodiment and preparation method thereof
Organic matter decomposing inoculant includes the component of following parts by weight：
6 parts of bacillus subtilis fermentation liquor, 41 parts of unwrapping wire bacteria solid fermentation thing, 7 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid
38 parts of fermentate, 9 parts of saccharomycetes to make fermentation liquid, 8 parts of rotten mosses ash；
The preparation method of above-mentioned organic matter decomposing inoculant is as follows：
Firstth, the preparation of bacillus subtilis fermentation liquor：
(1) preparation of seed liquor：The thalline that scraping one connects collarium bacillus subtilis from slant medium is inoculated into liquid
In body culture medium, shaking table culture 12h, rotating speed 200r/min, temperature are 30 DEG C, obtain the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L)：Tryptone 10, yeast extract 5, NaCl 10, water complements to
1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented, fermented under conditions of/h
Time is 20h, produces bacillus subtilis fermentation liquor；
Fermentation medium (g/L)：Corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9th, ammonium sulfate 1, K2HPO4
0.3rd, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
Secondth, the preparation of unwrapping wire bacteria solid fermentation thing：
(1) preparation of seed liquor：The thalline that scraping one connects collarium actinomyces from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L)：Potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5,
Ferrous sulfate heptahydrate 0.01, water complements to 1L；
(2) preparation of zymotic fluid：
By step (1) obtain seed liquor be inoculated into 10% (v/v) inoculum concentration in fermentation tank, rotating speed be 120 turns/
Min, temperature are 28 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, fermentation time 32h, produce and put
Line fermented liquid；
Actinomyces liquid fermentation medium (g/L)：Glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2,
Dipotassium hydrogen phosphate 0.4, epsom salt 0.05, water complements to 1L.
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is inoculated into 10% inoculum concentration (m/m)
In unwrapping wire bacteria solid fermentation culture medium, mix；2. heap processed：Length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Bar pile；3. fermentation management：When temperature of charge no longer improves after heating up, turning continues culture 5 days, produces actinomyces solid
Fermentate；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent)：Chicken manure (dry weight) 50%, mushroom residue 50%, mix.
3rd, the preparation of streptococcus acidi lactici fermented solution：
(1) preparation of seed liquor：The thalline that scraping one connects collarium lactic acid bacteria from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L)：Peptone 10.0, powdered beef 5.0, dusty yeast 4.0, glucose 20.0, tween
80 1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to
1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 28 DEG C, 200L fermentation tanks, throughput 6m3/ h, pH are sent out under conditions of being 6.1
Ferment, fermentation time 22h, produces streptococcus acidi lactici fermented solution；
Lactobacillus-fermented culture medium (g/L)：Peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato
Powder 0.25, Tween 80 0.1 are leached, water complements to 1L.
4th, the preparation of Aspergillus oryzae solid culture：
(1) preparation of seed liquor：The preparation of seed liquor：The thalline that scraping one connects collarium aspergillus oryzae from slant medium connects
Kind is into fluid nutrient medium, shaking table culture 12h, rotating speed 220r/min, and temperature is 28 DEG C, obtains the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L)：Urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05th, potassium dihydrogen phosphate 0.18, water complement to 1L.
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, during fermentation
Between be 30h, produce aspergillus oryzae zymotic fluid；
Aspergillus oryzae fermentation medium (g/L)：Urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride
0.5, ferric sulfate 0.5. water supplies 1L；
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is connect with 10-15% inoculum concentration (m/m)
Kind mixes into aspergillus oryzae solid fermentation culture medium；2. heap processed：Length 10m, wide 2-4m, height 30- is made in the material of mixing
40cm bar pile；3. fermentation management：When temperature of charge after heating up is not improving, turning continues culture 5 days, produces aspergillus oryzae
Solid fermentation thing；
Aspergillus oryzae solid fermentation culture medium is mushroom residue.
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor：The thalline that scraping one connects collarium saccharomycete from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L)：Yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, during fermentation
Between be 12h, produce saccharomycetes to make fermentation liquid；
Saccharomycetes to make fermentation culture medium (g/L)：Peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, chlorine
Change sodium 5, add water to 1L, pH 7.2；
6th, bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation thing, streptococcus acidi lactici fermented solution, aspergillus oryzae solid are sent out
Ferment thing, saccharomycetes to make fermentation liquid and rotten mosses ash, which mix in proportion, to be produced.
3 a kind of organic matter decomposing inoculant of embodiment and preparation method thereof
Organic matter decomposing inoculant includes the component of following parts by weight：
8 parts of bacillus subtilis fermentation liquor, 44 parts of unwrapping wire bacteria solid fermentation thing, 6 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid
32 parts of fermentate, 6 parts of saccharomycetes to make fermentation liquid；
The preparation method of above-mentioned organic matter decomposing inoculant is as follows：
Firstth, the preparation of bacillus subtilis fermentation liquor：
(1) preparation of seed liquor：The thalline that scraping one connects collarium bacillus subtilis from slant medium is inoculated into liquid
In body culture medium, shaking table culture 12h, rotating speed 200r/min, temperature are 30 DEG C, obtain the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L)：Tryptone 10, yeast extract 5, NaCl 10, water complements to
1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 100 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented, fermented under conditions of/h
Time is 18h, produces bacillus subtilis fermentation liquor；
Fermentation medium (g/L)：Corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9th, ammonium sulfate 1, K2HPO4
0.3rd, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
Secondth, the preparation of unwrapping wire bacteria solid fermentation thing：
(1) preparation of seed liquor：The thalline that scraping one connects collarium actinomyces from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L)：Potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5,
Ferrous sulfate heptahydrate 0.01, water complements to 1L；
(2) preparation of zymotic fluid：
By step (1) obtain seed liquor be inoculated into 10% (v/v) inoculum concentration in fermentation tank, rotating speed be 120 turns/
Min, temperature are 28 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, fermentation time 30h, produce and put
Line fermented liquid；
Actinomyces liquid fermentation medium (g/L)：Glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2,
Dipotassium hydrogen phosphate 0.4, epsom salt 0.05, water complements to 1L.
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is inoculated into 10% inoculum concentration (m/m)
In unwrapping wire bacteria solid fermentation culture medium, mix；2. heap processed：Length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Bar pile；3. fermentation management：When temperature of charge no longer improves after heating up, turning continues culture 4 days, produces actinomyces solid
Fermentate；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent)：Chicken manure (dry weight) 50%, mushroom residue 50%, mix.
3rd, the preparation of streptococcus acidi lactici fermented solution：
(1) preparation of seed liquor：The thalline that scraping one connects collarium lactic acid bacteria from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L)：Peptone 10.0, powdered beef 5.0, dusty yeast 4.0, glucose 20.0, tween
80 1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to
1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 110 turns/min in rotating speed, temperature is 28 DEG C, 200L fermentation tanks, throughput 6m3/ h, pH are sent out under conditions of being 6.5
Ferment, fermentation time 24h, produces streptococcus acidi lactici fermented solution；
Lactobacillus-fermented culture medium (g/L)：Peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato
Powder 0.25, Tween 80 0.1 are leached, water complements to 1L.
4th, the preparation of Aspergillus oryzae solid culture：
(1) preparation of seed liquor：The preparation of seed liquor：The thalline that scraping one connects collarium aspergillus oryzae from slant medium connects
Kind is into fluid nutrient medium, shaking table culture 12h, rotating speed 220r/min, and temperature is 28 DEG C, obtains the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L)：Urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05th, potassium dihydrogen phosphate 0.18, water complement to 1L.
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, during fermentation
Between be 28h, produce aspergillus oryzae zymotic fluid；
Aspergillus oryzae fermentation medium (g/L)：Urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride
0.5, ferric sulfate 0.5. water supplies 1L；
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is connect with 10-15% inoculum concentration (m/m)
Kind mixes into aspergillus oryzae solid fermentation culture medium；2. heap processed：Length 10m, wide 2-4m, height 30- is made in the material of mixing
40cm bar pile；3. fermentation management：When temperature of charge after heating up is not improving, turning continues culture 3 days, produces aspergillus oryzae
Solid fermentation thing；
Aspergillus oryzae solid fermentation culture medium is mushroom residue.
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor：The thalline that scraping one connects collarium saccharomycete from slant medium is inoculated into Liquid Culture
In base, shaking table culture 12h, rotating speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L)：Yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration
In, it is 120 turns/min in rotating speed, temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, during fermentation
Between be 12h, produce saccharomycetes to make fermentation liquid；
Saccharomycetes to make fermentation culture medium (g/L)：Peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, chlorine
Change sodium 5, add water to 1L, pH 7.0-7.2；
6th, bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation thing, streptococcus acidi lactici fermented solution, aspergillus oryzae solid are sent out
Ferment thing, saccharomycetes to make fermentation liquid and rotten mosses ash, which mix in proportion, to be produced.
The manufacturer of commercially available organic matter decomposing inoculant is Beijing photosynthetic organism Science and Technology Ltd. in test example.
Application test of 1 organic matter decomposing inoculant of the present invention of test example in wheat stalk returning
First, material and method
1st, test site：Experimental field it is located at Linyi Tancheng County；
2nd, test period：Test in 1 day July in 2016；
3rd, test specimen：Organic matter decomposing inoculant prepared by the embodiment of the present invention 1,2 and 3；
4th, test method：Linyi Tancheng is experimental field selected in, experimental field upper one batch of crop is wheat, tests ground
4 mu of product, is harvested on November 3rd, 2016 with harvester, and wheat highly stays in less than 5 centimetres during harvesting；
Experiment is divided into three treatment groups：Test group：Organic material composting prepared by stalk and the embodiment of the present invention 1,2 and 3
Agent；Control group：Stalk and equivalent clear water；
Test group：According to test requirements document every mu of organic material composting of the present invention with 2kg on the July 4 after stalk is completed
50kg fine earths are mixed in agent, are uniformly spread fertilizer over the fields on stalk, and it is fully absorbed moisture；
Control group：Only spray equivalent clear water, allows stalk to fully absorb moisture；
Field management：From decomposing agent on July 4 apply it is lower after, often arrive field observation and timely amount of makeup water, make stalk abundant
Moistening is kept, ridge is done between test group and control group to keep apart, it is not interconnected.
Observation item or index：1st, decomposed situation is observed within each 10 days；2nd, record start decomposed time and it is completely decomposed when
Between；
2nd, result of the test
1st, test group blackening since the 7th day, gently start to be broken once drawing with hand, especially eustipes part is easily broken off, straw
Stalk is just divided into several sections, the complete blackening at the 14th day, and fiber rots substantially thoroughly, without the control using decomposing agent of the present invention
Group, in the basic still yellow of the 10th day observation color, tenacity of fibre is very good, and two hands can't be broken with stem pulling section more energetically,
Just there is fragmentary blackening to observation in the 20th day, fiber starts to decay, and can be broken with stipes when pulling, but color mass-tone is still with yellowish-brown
Based on, to during complete blackening being August 20；As shown in table 1；Straw-returning can show with applying organic matter decomposing inoculant of the present invention
Write the content (p for improving the soil organism, alkali-hydrolyzable nitrogen, available potassium and available phosphorus<0.05).The present invention is compared with control group, speed
The content for imitating potassium and available phosphorus is as shown in table 2.
The available potassium of test group adds 79% compared with control group, and available phosphorus content improves compared with control group
31%.
1 decomposed record of table
The soil available nitrogen of table 2 and available phosphorus detection
Detection of the test example 2 to organic matter decomposing inoculant of the present invention
First, material and method
1st, test site：Liaocheng of Shandong Province Yanggu Xiang Yu organic fertilizers Co., Ltd；
2nd, test period：Test in August in 2016 1 day；
3rd, test specimen：Commercially available organic matter decomposing inoculant (Beijing photosynthetic organism Science and Technology Ltd.) and the embodiment of the present invention
1st, the organic matter decomposing inoculant prepared by 2 and 3；
4th, test method：
Test group 1：Organic matter decomposing inoculant prepared by rice husk chicken manure and embodiment 1,1kg is added in every cube of rice husk chicken manure
Embodiment 1 prepare organic matter decomposing inoculant；
Test group 2：Organic matter decomposing inoculant prepared by rice husk chicken manure and embodiment 2,1kg is added in every cube of rice husk chicken manure
Embodiment 2 prepare organic matter decomposing inoculant；
Test group 3：Organic matter decomposing inoculant prepared by rice husk chicken manure and embodiment 2,1kg is added in every cube of rice husk chicken manure
Embodiment 3 prepare organic matter decomposing inoculant；
Control group：Rice husk chicken manure and commercially available organic matter decomposing inoculant, the commercially available organic of 2kg is added in every cube of rice husk chicken manure
Material decomposing agent (m3/2KG)；
All keep the skin wet simultaneously to 55% or so (hold energy water outlet with hand one and do not drop), add 4kg to urinate per square rice husk chicken manure
Element.Every turning in three days keeps the skin wet once and according to actual conditions.
Observation item or index：1st, decomposed condition monitoring temperature is observed daily；2nd, decomposed completely rear survey germination percentage.
2nd, result of the test
The 2nd day can of organic matter decomposing inoculant prepared by the embodiment of the present invention 1,2 and 3 is warming up to 60 degree, corruption in 16 days
Ripe completion, germination percentage test being carried out with corn, germination percentage reaches 90%, and commercially available organic matter decomposing inoculant is warming up to 60 degree on the 4th day,
Germination percentage test is carried out with corn, germination percentage reaches 80%.
The organic matter decomposing inoculant of the present invention of test example 3 is to crop yield and the influence of soil texture
In order to preferably verify the effectiveness of organic matter decomposing inoculant of the present invention, decomposing agent is made using the embodiment of the present invention 1
Carry out proportion of crop planting experiment.
1. test material and method
(1) test period and place
Test site：Tai'an Shandong NongDa Fertilizer Science Co., Ltd Xing Run gardens experimental plot.
Test period：- 2016 years 2014.
Crop：Wheat, corn
(2) experimental design
Experiment sets 2 processing, and upper one batch of stalk is with site preparation returning to the field.Processing 1 is control, only with conventional fertilizer application, place
The conventional fertilizer application of organic matter decomposing inoculant+50% made from embodiment 1 of reason 2, calculates crops annual production, as shown in Table 3：Apply
After organic matter decomposing inoculant made from embodiment 1, there occurs significant changes, straw-returning to add organic matter for corn and wheat yield
Expect First Year, corn and wheat yield are significantly increased, and it is horizontal to basically reach middle production；Second Year corn and wheat yield persistently increase
Long, especially it is horizontal to basically reach middle high yield for corn yield.Tested by 2 years, compared with conventional fertilizer application, obvious effect of increasing production.
Influence of the different disposal of table 3 to corn wheat yield
As shown in Table 4：Organic matter decomposing inoculant continuous administration made from embodiment 1 is after 2 years, compared with the control, significantly
The soil weight is reduced, improves soil powder and soil capillary porosity percentage, and then improve the water conservation of soil
Nutrient preserving capability and aeration, make soil physical property be improved.
The influence of the different disposal soil mechanical composition of table 4
Claims (9)
1. a kind of organic matter decomposing inoculant, it is characterised in that organic matter decomposing inoculant includes following components：
Bacillus subtilis, actinomyces, lactic acid bacteria, aspergillus oryzae and saccharomycete.
2. organic matter decomposing inoculant according to claim 1, it is characterised in that organic matter decomposing inoculant includes following weight
The component of number：
Bacillus subtilis fermentation liquor 5-10 parts, unwrapping wire bacteria solid fermentation thing 40-45 parts, streptococcus acidi lactici fermented solution 5-10 parts, meter Qu
Mould solid fermentation thing 30-40 parts, saccharomycetes to make fermentation liquid 5-10 parts, rotten mosses ash 0-10 parts.
3. organic matter decomposing inoculant according to claim 1, it is characterised in that organic matter decomposing inoculant includes following weight
The component of number：Include the component of following parts by weight：
7 parts of bacillus subtilis fermentation liquor, 42 parts of unwrapping wire bacteria solid fermentation thing, 9 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid fermentation
34 parts of thing, 6 parts of saccharomycetes to make fermentation liquid, 4 parts of rotten mosses ash.
4. the preparation method of organic matter decomposing inoculant, specific as follows as described in claim any one of 1-3：
Firstth, the preparation of bacillus subtilis fermentation liquor：
(1) preparation of seed liquor：The thalline that scraping one connects collarium bacillus subtilis from slant medium is inoculated into liquid training
Support in base, shaking table culture 12h, rotating speed 200r/min, temperature is 30 DEG C, obtains the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L)：Tryptone 10, yeast extract 5, NaCl 10, water complements to 1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration,
Rotating speed is that 100-120 turns/min, and temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented, fermented under conditions of/h
Time is 18-20h, produces bacillus subtilis fermentation liquor；
Fermentation medium (g/L)：Corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9th, ammonium sulfate 1, K2HPO4
0.3rd, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
Secondth, the preparation of unwrapping wire bacteria solid fermentation thing：
(1) preparation of seed liquor：The thalline that scraping one connects collarium actinomyces from slant medium is inoculated into fluid nutrient medium,
Shaking table culture 12h, rotating speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L)：Potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5, seven water
Ferrous sulfate 0.01, water complements to 1L；
(2) preparation of zymotic fluid：
The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration, is 120 turns/min in rotating speed,
Temperature is 28 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, fermentation time 30-32h, produce unwrapping wire
Fermented liquid；
Actinomyces liquid fermentation medium (g/L)：Glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2, phosphoric acid
Hydrogen dipotassium 0.4, epsom salt 0.05, water complements to 1L.
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is inoculated into unwrapping wire with 10% inoculum concentration (m/m)
In bacteria solid fermentation culture medium, mix；2. heap processed：Length 10m, wide 2-4m, height 30-40cm bar is made in the material of mixing
Pile；3. fermentation management：When temperature of charge no longer improves after heating up, turning continues culture 3-5 days, produces actinomyces solid hair
Ferment thing；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent)：Chicken manure (dry weight) 50%, mushroom residue 50%, mix.
3rd, the preparation of streptococcus acidi lactici fermented solution：
(1) preparation of seed liquor：The thalline that scraping one connects collarium lactic acid bacteria from slant medium is inoculated into fluid nutrient medium,
Shaking table culture 12h, rotating speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L)：Peptone 10.0, powdered beef 5.0, dusty yeast 4.0, glucose 20.0, Tween 80
1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to 1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration,
Rotating speed is that 100-120 turns/min, and temperature is 28 DEG C, 200L fermentation tanks, throughput 6m3/ h, pH are sent out under conditions of being 6-6.5
Ferment, fermentation time 22-24h, produces streptococcus acidi lactici fermented solution；
Lactobacillus-fermented culture medium (g/L)：Peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato are leached
Powder 0.25, Tween 80 0.1, water complements to 1L.
4th, the preparation of Aspergillus oryzae solid culture：
(1) preparation of seed liquor：The preparation of seed liquor：The thalline that scraping one connects collarium aspergillus oryzae from slant medium is inoculated into
In fluid nutrient medium, shaking table culture 12h, rotating speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L)：Urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05th, potassium dihydrogen phosphate 0.18, water complement to 1L.
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration,
Rotating speed is 120 turns/min, and temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, fermentation time is
28-30h, produce aspergillus oryzae zymotic fluid；
Aspergillus oryzae fermentation medium (g/L)：Urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride 0.5, sulphur
Sour iron 0.5. water supplies 1L；
(3) solid culture：1. dispensing is fermented：The zymotic fluid that step (2) obtains is inoculated into 10-15% inoculum concentration (m/m)
In aspergillus oryzae solid fermentation culture medium, mix；2. heap processed：Length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Bar pile；3. fermentation management：When temperature of charge after heating up is not improving, turning continues culture 3-5 days, produces aspergillus oryzae and consolidates
Body fermentate；
Aspergillus oryzae solid fermentation culture medium is mushroom residue.
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor：The thalline that scraping one connects collarium saccharomycete from slant medium is inoculated into fluid nutrient medium,
Shaking table culture 12h, rotating speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L)：Yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) preparation of zymotic fluid：The seed liquor that step (1) obtains is inoculated into fermentation tank with 10% (v/v) inoculum concentration,
Rotating speed is 120 turns/min, and temperature is 30 DEG C, 200L fermentation tanks, throughput 6m3Fermented under conditions of/h, fermentation time is
12-14h, produce saccharomycetes to make fermentation liquid；
Saccharomycetes to make fermentation culture medium (g/L)：Peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, sodium chloride
5, add water to 1L, pH 7.0-7.2；
6th, by bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation thing, streptococcus acidi lactici fermented solution, Aspergillus oryzae solid culture,
Saccharomycetes to make fermentation liquid and rotten mosses ash, which mix in proportion, to be produced.
5. according to the method for claim 4, it is characterised in that in the preparation of bacillus subtilis fermentation liquor, rotating speed 110
Turn/min, fermentation time 19h.
6. according to the method for claim 4, it is characterised in that in the preparation of actinomycetes fermentation liquor, fermentation time 31h.
7. according to the method for claim 4, it is characterised in that in the preparation of streptococcus acidi lactici fermented solution, rotating speed be 110 turns/
Min, pH 6.2, fermentation time 23h.
8. according to the method for claim 4, it is characterised in that in the preparation of aspergillus oryzae zymotic fluid, fermentation time 29h.
9. according to the method for claim 4, it is characterised in that in the preparation of saccharomycetes to make fermentation liquid, fermentation time 13h.
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Address after: No. 249, Chuangye Road, High tech Zone, Feicheng City, Tai'an City, Shandong Province, 271600 Patentee after: Shandong Nongda Feiye Technology Co.,Ltd. Country or region after: China Address before: 271699 Feicheng high tech Development Zone, Tai'an, Shandong Patentee before: Shandong Nongda Feiye Technology Co.,Ltd. Country or region before: China |
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| CP03 | Change of name, title or address |