CN105462878B - A kind of efficient decomposing agent of feces of livestock and poultry and its fermentation process - Google Patents

A kind of efficient decomposing agent of feces of livestock and poultry and its fermentation process Download PDF

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CN105462878B
CN105462878B CN201510873042.0A CN201510873042A CN105462878B CN 105462878 B CN105462878 B CN 105462878B CN 201510873042 A CN201510873042 A CN 201510873042A CN 105462878 B CN105462878 B CN 105462878B
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culture
fermentation
poultry
livestock
feces
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CN105462878A (en
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金雪霞
侯中跃
路怀灯
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JIANGSU LVKE BIOTECHNOLOGY CO Ltd
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JIANGSU LVKE BIOTECHNOLOGY CO Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The present invention provides a kind of for the quickly efficient decomposing agent of odorless innoxious feces of livestock and poultry and its fermentation process, microorganism fungus kind component and carrier component including following mass ratio: the microorganism fungus kind group is divided into bacillus subtilis 10%~15%, bacillus licheniformis 10%~15%, saccharomycete 5%~15%, aspergillus niger 3%~8%, aspergillus niger 3%~8%, lactic acid producing lactobacillus acidophilus 10%~20%, lactic acid producing lactobacillus plantarum 10%~20% and carrier component are maltodextrin 10%~20%.It is mainly made of the Bacillus of all feeds grade and other microbial bacterias with carrier maltodextrin, it can be within 3-7 days time, quickly by feces of livestock and poultry, first decomposed fermentation becomes odorless substance first, then adding up fermentation again becomes the product of safe and harmlessization, eliminate pathogenic bacteria therein, helminth, weed seed etc. simultaneously can be used as organic fertilizer and its influence to plant germination index.

Description

A kind of efficient decomposing agent of feces of livestock and poultry and its fermentation process
Technical field
The present invention relates to a kind of decomposed leavening of feces of livestock and poultry, the efficient decomposing agent of especially a kind of feces of livestock and poultry and its fermentation Method.
Background technique
Developing livestock and poultry breeding industry can promote rural economy to increase, and increase farmers' income, can be with the swift and violent hair of aquaculture Exhibition, intensive degree is higher and higher, generates a large amount of feces of livestock and poultry therewith and is difficult to handle.It is maximum that breeding pollution has become China Pollution sources, crisis urban and rural environment, drinking water source, Agro-ecology, and become transmission source, it is detrimental to health.National environmental protection General bureau's investigation display, only 1999, it was 2.4 times of industrial solid castoff that China's feces of livestock and poultry yield, which is 1,900,000,000 tons,.Expert , it is expected that arriving the year two thousand twenty, the annual feces of livestock and poultry yield in China is up to 8,000,000,000 tons, if without effectively handling, it will be into one Step leads to the deterioration of environment.The Chinese government attaches great importance to breeding pollution governing problem, has made laws and has prevented and treated breeding pollution, local government Also it extracts special fund out from financial resources and is used for feces of livestock and poultry harmless treatment;Cultivation pollution of area source conduct is said by scientific research institution, expert Great research topic, it is intended to capture this problem;Some areas and large aquaculture family have felt deeply seriously to endanger brought by breeding pollution Evil starts investment processing feces of livestock and poultry.
On the other hand, even if using methane-generating pit processing technique large-scale farming field, it is also difficult to reach the standard of farmyard manure.Most Crucially simply use methane-generating pit processing fermentation, processing speed is slow and being also not thorough of reaction, pathogenic bacteria etc. are some Heavy metal can not it is degradable fall, act on above crop and will also result in secondary pollution etc..
Summary of the invention
Goal of the invention: to solve problems of the prior art, the present invention provides a kind of for quickly odorless innoxious The efficient decomposing agent of feces of livestock and poultry and its fermentation process, the efficient decomposing agent of the feces of livestock and poultry can subtract with quick composting feces of livestock and poultry Sustainable development is realized in few secondary pollution.
Technical solution: to realize the above-mentioned technical purpose, the invention proposes a kind of efficient decomposing agents of feces of livestock and poultry, comprising such as The component of lower parts by weight: 10~15 parts of bacillus subtilis, 10~15 parts of bacillus licheniformis, 5~15 parts of saccharomycete, aspergillus 3~8 parts of bacterium, 10~20 parts of lactic acid producing lactobacillus acidophilus, 10~20 parts of lactic acid producing lactobacillus plantarum and 10~20 parts of maltodextrin, Wherein, bacillus subtilis, bacillus licheniformis, saccharomycete, Aspergillus, lactic acid producing lactobacillus acidophilus, lactic acid producing plant cream bar Bacterium is as microorganism fungus kind component, and maltodextrin is as carrier.
As preferred embodiment, the efficient decomposing agent of feces of livestock and poultry includes the component of following parts by weight: viable bacteria 15 parts of the bacillus subtilis of 100,000,000,000 cfu/g or more of number, 100,000,000,000 cfu/g or more of viable count 15 parts of bacillus licheniformis, 5 parts of aspergillus niger, the viable count 5,000,000,000 of the saccharomyces cerevisiae 10% of 20,000,000,000 cfu/g or more of viable count, 5,000,000,000 cfu/g or more of viable count 5 parts of the aspergillus oryzae of cfu/g or more, 15 parts of the lactic acid producing lactobacillus acidophilus of 100,000,000,000 cfu/g or more of viable count, viable count 100,000,000,000 15 parts and 20 parts of maltodextrin of the lactic acid producing lactobacillus plantarum of cfu/g or more.
Preferably, the viable count sum of bacillus subtilis and bacillus licheniformis is greater than 10,000,000,000 cfu/g, saccharomyces cerevisiae Viable count be greater than 5,000,000,000 cfu/g, aspergillus oryzae and aspergillus niger viable count sum are greater than 3,000,000,000 cfu/g, lactic acid producing lactobacillus acidophilus It is greater than 10,000,000,000 cfu/g with lactic acid producing lactobacillus plantarum viable count sum.
Preferably, the efficient decomposing agent of the feces of livestock and poultry further includes in rice bran, corncob, dregs of beans or soluble starch Any one or a few mixture, and as carrier.
Present invention further proposes the efficient decomposing agent of feces of livestock and poultry fermentation process, include the following steps:
(1) it is in mass ratio 1~1.5: 10~15 by the decomposing agent and wheat bran or corn flour, strain mixing is made Object is spare, it is therefore an objective to convenient for stirring evenly;
(2) it is 1~1.5: 200~250 according to mass ratio by strain mixture and feces of livestock and poultry, is uniformly mixed, poultry is made Poultry manure mixed material, and the moisture content for controlling mixed material by the loose stacking of material and is sealed by fermentation 60%;
(3) mixed material being sealed by fermentation in step (2) is covered into one layer of modeling on mixed material surface layer while fermentation Expect that film, control fermentation time are 5-15 days.
Feces of livestock and poultry decomposing agent of the invention it is characterized in that by its following mass ratio microorganism fungus kind component and vehicle group It is grouped as: containing the bacillus subtilis 15% of 100,000,000,000/g or more, contain the bacillus licheniformis 15% of 100,000,000,000/g or more, content The saccharomyces cerevisiae 10% of 20000000000/g or more, the aspergillus niger 5% of 5,000,000,000/g or more of content, the aspergillus oryzae of 5,000,000,000/g or more of content The lactic acid producing lactobacillus acidophilus 15% of 5%, 100,000,000,000/g or more of content, the lactic acid producing lactobacillus plantarum of 100,000,000,000/g or more of content 15%;Carrier component is maltodextrin 20%.
The utility model has the advantages that compared with prior art, the present invention has the advantage that
(1) the efficient decomposing agent of feces of livestock and poultry of the invention has fermenting speed fast, ferments the features such as thorough, in winter It needs can thoroughly eliminate stink within 5~7 days, and can control the requirement for reaching innoxious index within 3 days in summer, and There is the distinctive sour taste of fermentation to give out, then some causes such as 20 days Escherichia coli that can be completely eliminated in excrement of accumulative fermentation Germ, and can be used as organic fertilizer material and the influence to plant germination index;
(2) leavening of the invention is compound using more bacterium and living bacteria count is high, reaches 45,000,000,000/g or more, is generally to raise 800 times or more of poultry manure decomposing agent.Currently, the viable bacteria amount that organic matter decomposing inoculant as defined in the Ministry of Agriculture of China contains be >= Bacillus subtilis contained by 0.5 hundred million/g (GB20287-2006) present invention and bacillus licheniformis sum are greater than 15,000,000,000/g, wine brewing Yeast is greater than 10,000,000,000/g, and aspergillus oryzae and aspergillus niger sum are greater than 5,000,000,000/g, lactic acid producing lactobacillus acidophilus and lactic acid producing plant cream bar Bacterium sum is greater than 15,000,000,000/g.
(3) fungi, bacterium, mould etc. mainly are combined in product of the present invention, mutually not antagonism, synergistic effect, no Only there is powerful decomposed effect to feces of livestock and poultry, and also breeds a large amount of function bacteriums during the fermentation and generate a variety of special efficacy generations It thanks to product, after feces of livestock and poultry ferments again, there is the distinctive sour taste that ferments.
It (4), can be as the ecology of field crops after using the present invention efficiently abundant fermented livestock excrement of decomposing agent agent Organic fertilizer.
Specific embodiment
Raw material strain of the invention can also be prepared by purchasing acquisition in the market by following production technology: raw The microorganism fungus kind of production is all from China Committee for Culture Collection of Microorganisms's common micro-organisms center and Chinese agriculture Microbiological Culture Collection administrative center.Wherein Bacillus subtilis strain number or deposit number are (ACCC 11088), lichens Perhaps deposit number is that (ACCC 06149) lactic acid producing Lactobacillus acidophilus species number or deposit number is to Bacillus number (ACCC 05489) aspergillus niger strain number or deposit number be (CGMC 3.0316), be the Ministry of Agriculture allow using it is beneficial Microbial strains announce No. 2045 file in 2013 referring to The Ministry of Agriculture of the People's Republic of China, MOA.
Wherein bacillus subtilis, bacillus licheniformis culture process are as follows: respective classical nutritional agar slant test tube bacterium Kind (culture 4 days) → choose bacterium colony strain with conspicuous characteristics, then carries out following steps:
(1) laboratory first order seed culture (about 3L) is connect:
Culture medium: yeast extract 6g, beef extract 15g, peptone 30g, glucose 30g, epsom salt 1.5g, phosphoric acid hydrogen 3L water is added in dipotassium 1.5g sodium chloride 15g, after completely dissolution, with sodium hydroxide tune pH value to 7.0, dispenses to conical flask, in 125 DEG C of sterilizing 20min.Inoculation: it after the culture medium for bacterium of having gone out is cooling, is inoculated with oese.Condition of culture: the control of shaking table temperature exists It 36 ± 1 DEG C, revolving speed 190R/min, cultivates 18-20 hours.
(2) workshop secondary seed tank culture (about 300L):
Culture medium: yeast powder 1.5kg, Dried Corn Steep Liquor Powder 6kg, glucose 6kg, sodium chloride 1.5kg, potassium dihydrogen phosphate, 150g dipotassium hydrogen phosphate 600g.With sodium hydroxide tune pH value to 7.0.Medium sterilization controls 120 DEG C 20~30 points of 0.1mPa Clock.Inoculation: flame inoculation, 300~500ml of inoculum concentration.Condition of culture: 36 ± 1 DEG C of tank temperature of control, tank press 0.06 ± 0.01mPa, Ventilatory capacity 100~150L/ minutes, stirring was opened after inoculation, 80~120 revs/min of speed of agitator, is cultivated 16~20 hours.Culture Standard: microscopy thallus dye color depth, thalli growth uniformly, without miscellaneous bacteria, gemma is not yet formed
(3) workshop three grade fermemtation tank culture (about 3000L):
Culture medium: soybean powder 28kg, glucose 60kg, starch 20kg, Dried Corn Steep Liquor Powder 60kg, yeast powder 15kg, albumen Peptone 28kg, manganese sulfate 1.1kg, magnesium sulfate 1kg, sodium chloride 15kg, potassium dihydrogen phosphate 1.5kg are cultivated with sodium hydroxide tune pH7.0 Base sterilizing control 120 DEG C of 0.1mPa 20~30 minutes.Culture transferring: 150~200L of inoculum concentration.Condition of culture: control tank temperature 36 ± 1 DEG C, tank press 0.06 ± 0.01mPa, ventilatory capacity 700-750L/min, open stirring after culture transferring, 100-150 revs/min of speed of agitator, Culture 18-24 hours.The intermediate control of fermentation: after 8 hours, pH and OD value is surveyed in sampling in every 2 hours.Fermentation termination control: work as dilution 10 times of OD values continuous 1.5 or more, and it is on a declining curve, and pH value persistently rises, and sampling carries out microscopy, the dyeing coloring of microscopy thallus Not deep, thalli growth uniformly, without miscellaneous bacteria, almost all forms gemma, can terminate fermentation.
(4) centrifuge is centrifuged
Final fermentation liquid is driven into centrifuge to be centrifuged, obtains solidliquid mixture.
(5) it is spray-dried
After mixing centrifugation sufficiently, high-temperature spray is carried out by spray drying large scale equipment, solid product is high activity, height The bacillus subtilis or bacillus licheniformis of content.
Wherein lactic acid producing lactobacillus acidophilus, lactic acid producing lactobacillus plantarum culture process are as follows: respective MRS typical case's slant tube Strain (culture 4 days) → choose bacterium colony strain with conspicuous characteristics, then carries out following steps:
(1) laboratory first order seed culture (2.5L)
Culture medium: yeast extract 30g, beef extract 30g, peptone 30g, glucose 60g, magnesium sulfate 1.5g, phosphoric acid hydrogen two Potassium 6g, sodium acetate 15g, ammonium citrate 6g.2.5L water is added, after completely dissolution, with sodium hydroxide tune pH value to 7.0, packing is extremely Conical flask, in 125 DEG C of sterilizing 20min.
Inoculation: it after the culture medium for bacterium of having gone out is cooling, is inoculated with oese.
Condition of culture: biochemical cultivation case temperature is controlled at 36 ± 1 DEG C, stationary culture 36-48 hours.
(2) workshop secondary seed tank culture (250L)
Culture medium: ammonium sulfate 150g, sodium nitrate 150g, dipotassium hydrogen phosphate 600g, magnesium sulfate 150g, Dried Corn Steep Liquor Powder 2.4kg, peptone 3kg, glucose 6kg, sodium chloride 150g, ammonium citrate 600g.Fixing fabric structure is in 250- after pH value 7.0 disappears 300L.Medium sterilization control 0.1mPa120 DEG C 20~30 minutes.
Inoculation: flame inoculation, inoculum concentration 2-2.5L.
Condition of culture: 36 ± 1 DEG C of tank temperature of control, tank press 0.06 ± 0.01mpa, stuffy, do not open stirring, stationary culture 24 ~48 hours.
Training status: microscopy thallus dye color depth, thalli growth uniformly, without miscellaneous bacteria.
(3) workshop three grade fermemtation tank culture (2500L)
Culture medium: ammonium sulfate 1.5kg, sodium nitrate 1.5kg, dipotassium hydrogen phosphate 6kg, magnesium sulfate 1.5kg, Dried Corn Steep Liquor Powder 24kg peptone 30kg, glucose 60kg, sodium chloride 1.5kg.Fixing fabric structure is in 2500L or so after pH value 7.0 disappears.Culture medium goes out Bacterium control 0.1mPa120 DEG C 20~30 minutes.
Culture transferring: 300~320L of inoculum concentration.
Condition of culture: 36 ± 1 DEG C of tank temperature of control, tank press 0.06 ± 0.01mpa, stuffy, do not open stirring, stationary culture 24 ~48 hours.
The intermediate control of fermentation: after 16 hours, pH and OD value is surveyed in sampling in every 2 hours.
Fermentation termination control: when diluting 10 times of OD values continuous 0.3 or more, and on a declining curve, pH value 3.5 is hereinafter, simultaneously And it is in rising trend, it samples and carries out microscopy, the dyeing coloring of microscopy thallus is not deep, thalli growth is uniform, without miscellaneous bacteria, can terminate hair Ferment.
(4) centrifuge is centrifuged
Final fermentation liquid is driven into tube centrifuge to be centrifuged, obtains solidliquid mixture.
(5) it is freeze-dried
After mixing centrifugation sufficiently, frozen drying is carried out by freeze drying equipment, solid product is high activity, height The lactobacillus acidophilus of content or lactobacillus plantarum.
Below by specific embodiment, the present invention will be described in detail.
Specific embodiment: further summarize is made to the present invention With reference to embodiment.
Embodiment 1
The present embodiment proposes a kind of feces of livestock and poultry decomposing agent, as the microorganism fungus kind of following (shown in table 1) mass ratio Component and carrier component composition (preparation method of each microorganism is as described above):
1 embodiment of table, 1 product viable count
Embodiment 2
The viable count of heretofore described microorganism fungus kind is compounded using the combination of the ratio of bacterium and fungi 2: 1, Middle bacillus subtilis and bacillus licheniformis sum are greater than 15,000,000,000/g, and saccharomyces cerevisiae is greater than 10,000,000,000/g, aspergillus oryzae and black song Mould sum is greater than 5,000,000,000/g, and lactic acid producing lactobacillus acidophilus and lactic acid producing lactobacillus plantarum sum are greater than 15,000,000,000/g.Finally it is re-dubbed Content is the efficient decomposing agent product of feces of livestock and poultry of 45,000,000,000/g.
The method of the decomposed fermented livestock excrement of embodiment 3.
Compost (fertilizer proportion per ton) method: 200 kilograms of stalks and 800 kilograms of feces of livestock and poultry are uniformly mixed, then The product of the preparation of 150g the present embodiment 1 is sprinkled, moisturizing to final moisture content is 50%~60% after heap is good, stirs evenly, piles width About 2-3 meters, high about 0.8-1.2 meters of compost, length is unlimited.50 DEG C, which are raised to, to fermentation temperature starts turnings, later daily one It is secondary.The completion that can ferment in spring and summer autumn 5 days or so, winter 10 days or so can be used.
Farm 5% or so is added to poultry if any can adding for rice bran, corn flour etc together with strain mixed diluting In poultry manure.
Use the physicochemical data monitoring result after product of the present invention fermented livestock excrement:
By above-mentioned fermentation process: feces of livestock and poultry 800kg, stalk 200kg, corn flour or rice bran 50kg, the addition present invention Feces of livestock and poultry decomposing agent 150g, control water content is 60%, and carrying out fermentation, the results are shown in Table 2 in 10 days or so.
Table 2
Illustrate: E. coli detection method is detected in fermentation fecal specimens using dilution plate rubbing method with LB culture medium Escherichia coli quantity.
Here is to utilize the product of the present invention fermented cow dung shadow as organic fertilizer to germination index and physicochemical property after an action of the bowels Ring
1 materials and methods
1.1 experimental material
There is provided by Jiangsu Bioisystech Co., Ltd, this experiment sets 2 processing, every processing repeatedly 3 this.
Processing 1: product of the present invention is not connect.Cattle manure is mixed into straw powder, paddy meal or rice bran etc, adjusts mixture Moisture content stirs evenly cattle manure and straw powder, paddy meal or rice bran etc to 60%.Above-mentioned material heap is in heaps, it is wide Bottom width 2.8m is spent, height 1.5m's is trapezoidal, and surface makes real.
Processing 2: inoculation product of the present invention.Cattle manure is mixed into straw powder, paddy meal or rice bran etc, adjusts mixture Moisture content adds product 150g to 60%, by every 1000kg feces of livestock and poultry, and 2kg brown sugar is added.Mixture and microbial inoculum are stirred It mixes uniformly.Above-mentioned material heap is in heaps, and width bottom width 2.8m, height 1.5m's is trapezoidal, and surface makes real.
Compost temperature measurement, temperature measuring point are away from the surface layer depths 25-30cm in compost, after thermometer is inserted into compost 5-10 minutes Reading, each compost measures 4 points in different location, and calculates mean temperature, while recording weather condition and temperature, from material Start within second day after piling up, METHOD FOR CONTINUOUS DETERMINATION when every morning 10.Temperature is begun to decline after compost temperature rises to 60 DEG C or more Shi Caiyong turner carries out turning, primary every the uniform turning of 2-3d, until temperature no longer rises after turning.
Influence of the 1.2 inoculation product of the present invention to cattle manure compost temperature
It is inoculated with the efficient decomposing agent of green section compost temperature in compost 2d to begin to rise to 57 DEG C or more, and is not inoculated with pair Just reach 55 DEG C or more in 8d according to processing.It is inoculated with efficient decomposing agent processing compost heap temperature and maintains 55 DEG C or more of total number of days and reach By 13 days, wherein the total number of days for maintaining 60 DEG C or more is 8 days;And bacterium control treatment compost heap temperature is not connect and maintains 55 DEG C or more Total number of days be only 9 days, the total number of days for maintaining 60 DEG C or more is only 3 days, relatively be inoculated with decomposing agent processing respectively lack 4 days and 6 It.Show that being inoculated with green section efficient decomposing agent of the invention is able to maintain that the time of high temperature is longer, is conducive to reach harmless treatment The purpose of pathogen and weed seed is eliminated in the process.
Influence of the 1.3 inoculation product of the present invention to cow dung compost germination index
As seen from the results in Table 3, the germination index of excrement is only 22.8% before fermenting, and is inoculated with the invention product and ferments 30 days Afterwards, excrement stalk germination index reaches 104.2%, and do not connect bacterium control is only 69.7%.Consulting literatures are generally acknowledged that when hair When bud index reaches 80%-85%, compost has reached decomposed success and does not have toxicity.
Table 3
Germination index (%) Organic carbon (%) Full nitrogen (%) Full phosphorus (%)
Excrement before fermenting 22.8c 37.1a 1.24b 1.03b
Excrement after fermentation 104.2a 29.2b 1.59a 1.47a
Control 69.7b 32.7b 1.37b 1.41a
From the above results, raw material total content of organic carbon is 37.1% before fermenting, and is inoculated with invention product fermentation 30d Afterwards, the organic carbon of compost is reduced to 29.2%, and control treatment is 32.7%;Raw material total nitrogen content is 1.24% before fermenting, inoculation The invention product ferments after 30d, and the full nitrogen of compost increases to 1.59%, and control treatment is 1.37%;The full phosphorus of raw material before fermenting Content is 1.03%, and after being inoculated with invention product fermentation 30d, the content of tatal phosphorus of compost is 1.47%, and control treatment is 1.41%.The C/N of raw material is 29.9 before fermenting, and after being inoculated with invention product fermentation 30d, the C/N of compost is reduced to 18.3, and compares Processing is 23.8.

Claims (3)

1. a kind of efficient decomposing agent of feces of livestock and poultry, which is characterized in that the component comprising following parts by weight: viable count 100,000,000,000 15 parts of the bacillus subtilis of cfu/g, 15 parts of the bacillus licheniformis of 100,000,000,000 cfu/g or more of viable count, viable count 20,000,000,000 10 parts of the saccharomyces cerevisiae of cfu/g or more, 5 parts of the aspergillus niger of 5,000,000,000 cfu/g or more of viable count, 5,000,000,000 cfu/g's or more of viable count 5 parts of aspergillus oryzae, 15 parts of the lactic acid producing lactobacillus acidophilus of 100,000,000,000 cfu/g or more of viable count, 100,000,000,000 cfu/g's or more of viable count 15 parts and 20 parts of maltodextrin of lactic acid producing lactobacillus plantarum,
Bacillus subtilis, bacillus licheniformis culture process are as follows: respective classical nutritional agar slant test tube strains, culture 4 It, chooses bacterium colony strain with conspicuous characteristics, then carries out following steps:
(1) laboratory first order seed culture 3L is met:
Culture medium: yeast extract 6g, beef extract 15g, peptone 30g, glucose 30g, epsom salt 1.5g, dipotassium hydrogen phosphate 3L water is added in 1.5g, sodium chloride 15g, after completely dissolution, with sodium hydroxide tune pH value to 7.0, dispenses to conical flask, in 125 DEG C sterilizing 20min;
Inoculation: it after the culture medium for bacterium of having gone out is cooling, is inoculated with oese;
Condition of culture: shaking table temperature is controlled at 36 ± 1 DEG C, 190 turns/min of revolving speed, is cultivated 18-20 hours;
(2) workshop secondary seed tank culture 300L:
Culture medium: yeast powder 1.5kg, Dried Corn Steep Liquor Powder 6kg, glucose 6kg, sodium chloride 1.5kg, potassium dihydrogen phosphate 150g, phosphorus Sour hydrogen dipotassium 600g;With sodium hydroxide tune pH value to 7.0;Medium sterilization control 0.1mPa, 120 DEG C 20~30 minutes;It connects Kind: flame inoculation, 300~500ml of inoculum concentration;Condition of culture: 36 ± 1 DEG C of tank temperature of control, tank press 0.06 ± 0.01mPa, ventilation Amount 100~150L/ minutes is opened stirring, 80~120 revs/min of speed of agitator, is cultivated 16~20 hours after inoculation;Training status: Microscopy thallus dye color depth, thalli growth uniformly, without miscellaneous bacteria, gemma is not yet formed;
(3) workshop three grade fermemtation tank culture 3000L:
Culture medium: soybean powder 28kg, glucose 60kg, starch 20kg, Dried Corn Steep Liquor Powder 60kg, yeast powder 15kg, peptone 28kg, manganese sulfate 1.1kg, magnesium sulfate 1kg, sodium chloride 15kg, potassium dihydrogen phosphate 1.5kg, with sodium hydroxide tune pH7.0, culture Base sterilizing control 0.1mPa, 120 DEG C 20~30 minutes;Culture transferring: 150~200L of inoculum concentration;Condition of culture: control tank temperature 36 ± 1 DEG C, tank press 0.06 ± 0.01mPa, ventilatory capacity 700-750L/min, open stirring after culture transferring, 100-150 revs/min of speed of agitator, Culture 18-24 hours;
The intermediate control of fermentation: after 8 hours, pH and OD value is surveyed in sampling in every 2 hours;
Fermentation termination control: when diluting 10 times of OD values continuous 1.5 or more, and on a declining curve, pH value persistently rises, sample into Row microscopy, the dyeing coloring of microscopy thallus is not deep, thalli growth is uniform, without miscellaneous bacteria, and almost all forms gemma, can terminate hair Ferment;
(4) centrifuge is centrifuged
Final fermentation liquid is driven into centrifuge to be centrifuged, obtains solidliquid mixture;
(5) it is spray-dried
After mixing centrifugation sufficiently, high-temperature spray is carried out by spray drying large scale equipment, solid product is high activity, high-content Bacillus subtilis or bacillus licheniformis;
Wherein lactic acid producing lactobacillus acidophilus, lactic acid producing lactobacillus plantarum culture process are as follows: respective MRS typical case slant tube bacterium Kind, it cultivates 4 days, chooses bacterium colony strain with conspicuous characteristics, then carry out following steps:
(1) laboratory first order seed culture 2.5L
Culture medium: yeast extract 30g, beef extract 30g, peptone 30g, glucose 60g, magnesium sulfate 1.5g, dipotassium hydrogen phosphate 6g, Sodium acetate 15g, ammonium citrate 6g;2.5L water is added, after completely dissolution, with sodium hydroxide tune pH value to 7.0, packing to triangle is burnt Bottle, in 125 DEG C of sterilizing 20min;
Inoculation: it after the culture medium for bacterium of having gone out is cooling, is inoculated with oese;
Condition of culture: biochemical cultivation case temperature is controlled at 36 ± 1 DEG C, stationary culture 36-48 hours;
(2) workshop secondary seed tank culture 250L
Culture medium: ammonium sulfate 150g, sodium nitrate 150g, dipotassium hydrogen phosphate 600g, magnesium sulfate 150g, Dried Corn Steep Liquor Powder 2.4kg, egg White peptone 3kg, glucose 6kg, sodium chloride 150g, ammonium citrate 600g;PH value 7.0, fixing fabric structure is in 250-300L after disappearing;Culture Base sterilizing control 0.1mPa, 120 DEG C 20~30 minutes;
Inoculation: flame inoculation, inoculum concentration 2-2.5L;
Condition of culture: 36 ± 1 DEG C of tank temperature of control, tank press 0.06 ± 0.01mpa, stuffy, do not open stirring, stationary culture 24~48 Hour;
Training status: microscopy thallus dye color depth, thalli growth uniformly, without miscellaneous bacteria;
(3) workshop three grade fermemtation tank culture 2500L
Culture medium: ammonium sulfate 1.5kg, sodium nitrate 1.5kg, dipotassium hydrogen phosphate 6kg, magnesium sulfate 1.5kg, Dried Corn Steep Liquor Powder 24kg, Peptone 30kg, glucose 60kg, sodium chloride 1.5kg;PH value 7.0, fixing fabric structure is in 2500L after disappearing;Medium sterilization control 0.1mPa, 120 DEG C 20~30 minutes;
Culture transferring: 300~320L of inoculum concentration;
Condition of culture: 36 ± 1 DEG C of tank temperature of control, tank press 0.06 ± 0.01mpa, stuffy, do not open stirring, stationary culture 24~48 Hour;
The intermediate control of fermentation: after 16 hours, pH and OD value is surveyed in sampling in every 2 hours;
Fermentation termination control: when diluting 10 times of OD values continuous 0.3 or more, and on a declining curve, pH value 3.5 is hereinafter, and be in Ascendant trend samples and carries out microscopy, and the dyeing coloring of microscopy thallus is not deep, thalli growth is uniform, without miscellaneous bacteria, can terminate fermentation;
(4) centrifuge is centrifuged
Final fermentation liquid is driven into tube centrifuge to be centrifuged, obtains solidliquid mixture;
(5) it is freeze-dried
Mixing centrifugation sufficiently after, by freeze drying equipment carry out frozen drying, solid product be lactobacillus acidophilus or Person's lactobacillus plantarum.
2. the efficient decomposing agent of feces of livestock and poultry according to claim 1, which is characterized in that the efficient decomposing agent of feces of livestock and poultry It further include any one or a few the mixture in rice bran, corncob, dregs of beans or soluble starch.
3. the fermentation process of the efficient decomposing agent of the described in any item feces of livestock and poultry of claim 1~2, which is characterized in that including such as Lower step:
(1) by the decomposing agent and wheat bran or corn flour, it is in mass ratio 1~1.5: 10~15, it is standby that strain mixture is made With;
(2) it is 1~1.5: 200~250 according to mass ratio by strain mixture and feces of livestock and poultry, is uniformly mixed, animal dung is made Just mixed material, and the moisture content for controlling mixed material by the loose stacking of material and is sealed by fermentation 60%;
(3) that the mixed material being sealed by fermentation in step (2) is covered one layer of plastics on mixed material surface layer while fermentation is thin Film, control fermentation time are 5~15 days.
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CN106747690A (en) * 2017-02-16 2017-05-31 江苏里下河地区农业科学研究所 A kind of cow dung compost promotees rotten guarantor nitrogen microbial inoculum and application process
CN107177527A (en) * 2017-05-27 2017-09-19 兰溪市酉泽饲料技术服务有限公司 Feces of livestock and poultry high-efficiency ferment and its fermentation process
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