CN107881136A - The method of composite bacterial and preparation method thereof and degraded animal waste - Google Patents
The method of composite bacterial and preparation method thereof and degraded animal waste Download PDFInfo
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- CN107881136A CN107881136A CN201711445083.5A CN201711445083A CN107881136A CN 107881136 A CN107881136 A CN 107881136A CN 201711445083 A CN201711445083 A CN 201711445083A CN 107881136 A CN107881136 A CN 107881136A
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- bacillus
- animal waste
- preparation
- composite bacterial
- lactic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of composite bacterial, including elongated bacillus, oblong bacillus, yeast and Bacillus acidi lactici, calculate by mass percentage, elongated bacillus：Oblong bacillus：Yeast：Bacillus acidi lactici=1:1:1.5:0.4.The invention also discloses the preparation method of composite bacterial：Comprise the following steps：<1>The preparation of bacillus；<2>The preparation of saccharomycete；<3>The preparation of lactic acid bacteria；<4>Mixed culture.The invention also discloses the method for degraded animal waste.Excrement after this fermentation, excrement quick composting can be made, harmful material is changed into the utilizable organic nutrition of plant, the side effects such as burn will not be produced to any plant.
Description
Technical field
The present invention relates to organic fertilizer technical field, more particularly to composite bacterial and preparation method thereof and animal waste of degrading
Method.
Background technology
After the most frequently used processing method of animal waste is heap fermentation, used as farmland organic fertilizer, but with big rule
Mould industrializes the development of animal husbandry, and animal waste amount is big and concentrates, and place needed for the conventional existing method in place is big, and environmental pollution is tight
Weight.
Organic fertilizer is good compared with inorganic fertilizer in terms of culture soil fertility and fertilizing soil, and it is logical for common are machine fertilizer at present
Cross barnyard manure, night soil, compost, make compost etc. based on fermenting raw materials.The content of organic matter of fowl and animal excrement is more, containing more
Ammonification microorganism, oxygen deficiency is easily caused anaerobic fermentation in digest process, and the humus of formation is less, and heating rate
Slowly, fermenting speed is slow, and efficiency is low, and yield is few, and fertile matter is poor, and fertilizer efficiency is poor, is unfavorable for improving the yield of crops.
The content of the invention
The defects of to overcome above-mentioned prior art to exist, the invention discloses a kind of composite bacterial, including elongated gemma bar
Bacterium, oblong bacillus, yeast and Bacillus acidi lactici, are calculated by mass percentage, elongated bacillus：Oblong gemma
Bacillus：Yeast：Bacillus acidi lactici=1:1:1.5:0.4.
Its further technical scheme is：Elongated bacillus size is 1.4-1.8um
×3.5-4.6um。
Its further technical scheme is：Oblong bacillus size is 0.4-0.9um × 0.8-1.5um.
The preparation method of composite bacterial：Comprise the following steps：
<1>The preparation of bacillus
With beef-protein medium, PH is in 7.2-7.4, first autoclaving 30 minutes, then inoculated and cultured, after inoculation
Constant temperature 30-38 degree culture 48 hours, the preparation of bacillus are completed；
<2>The preparation of saccharomycete
Saccharomycete is individually cultivated 24 hours, prepared by saccharomycete completes；
<3>The preparation of lactic acid bacteria
Lactic acid bacteria is individually cultivated 24-38 hours, prepared by lactic acid bacteria completes；
<4>Mixed culture
The bacillus completed, saccharomycete, lactic acid bacteria these three bacterium mixed culture 48-60 hours, composite bacterial will be prepared
Prepare and complete.
The invention also discloses the method using above-mentioned composite bacterial degraded animal waste, comprise the following steps：(1) will be as
10-25 kilograms of composite bacterial described in claim 1 is sprayed on every cubic metre of animal waste；(2) after having sprayed, by family's animal manure
Just it is well mixed with composite bacterial；(3) animal waste after will be well mixed carries out heap fermentation；(4) after fermenting 3-5 days, family
Animal manure just can be decomposed.Elongated bacillus, oblong bacillus production method are entered with general bacteria culture media can
Row culture, cultivation temperature are 28-37 DEG C.
Its further technical scheme is：Composite bacterial, bacterial content is every milliliter more than 2,000 ten thousand.
Its further technical scheme is：Elongated bacillus size is 1.4-1.8um × 3.5-4.6um.
Its further technical scheme is：Oblong bacillus size is 0.4-0.9um × 0.8-1.5um.
The invention has the advantages that：
(1) can animal waste is abundant decomposed and degraded, and the plant of small molecule nutrition supplying can be become in a short time
Thing fully absorbs utilization, while in processing procedure, can bottom out its ammonia taste, and 3-8 hour excrement is smelly after spraying
Taste can disappear, and 12 hours temperature can rise to 60 ° or so, and can complete fermentation is completed within 3-5 days.
(2) nutrition in animal waste can be made all quickly to discharge, beneficial to plant Omnibearing absorbing, supply plant
Whole nutrition required for growing, while plant will not produce the side effects such as any burn after use.
(3) lactic acid bacteria mainly plays regulatory role in this mixed liquor.
Embodiment
Preferably to illustrate the present invention, the present invention is further illustrated presently in connection with embodiment.
Embodiment one
A kind of composite bacterial, including elongated bacillus, oblong bacillus, yeast and Bacillus acidi lactici, by quality
Percentage calculates, elongated bacillus：Oblong bacillus：Yeast：Bacillus acidi lactici=1:1:1.5:0.4.
Elongated bacillus size is 1.4um × 3.5um.
Oblong bacillus size is 0.9um × 1.5um.
The preparation method of composite bacterial：Comprise the following steps：
<1>The preparation of bacillus
With beef-protein medium, PH is in 7.2-7.4, first autoclaving 30 minutes, then inoculated and cultured, after inoculation
35 degree of constant temperature is cultivated 48 hours, and the preparation of bacillus is completed；
<2>The preparation of saccharomycete
Saccharomycete is individually cultivated 24 hours, prepared by saccharomycete completes；
<3>The preparation of lactic acid bacteria
Lactic acid bacteria is individually cultivated 30 hours, prepared by lactic acid bacteria completes；
<4>Mixed culture
The bacillus completed, saccharomycete, lactic acid bacteria these three bacterium will be prepared to be mixed 55 hours, prepared by composite bacterial
Complete.
Using the method for above-mentioned composite bacterial degraded animal waste, comprise the following steps：(1) it is above-mentioned composite bacterial 10 is public
Jin is sprayed on every cubic metre of animal waste；(2) after having sprayed, animal waste and composite bacterial are well mixed；(3) will mixing
Animal waste after uniformly carries out heap fermentation；(4) after fermenting 3 days, animal waste can be decomposed.
Composite bacterial, bacterial content is at 2,000 ten thousand every milliliter.
The embodiment has the advantages that：(1) this compound bacteria, can be in the short time after spraying application on animal waste
It can bottom out its ammonia taste, 3-4 hours, temperature can rise to 60 degree or so within 12 hours, 3-5 just without the stink of excrement
It can complete fermentation completing technology effect；(2) excrement after this fermentation, can make excrement quick composting, harmful thing
Matter changes into the utilizable organic nutrition of plant, will not produce the side effects such as burn to any plant；(3) lactic acid bacteria is this
Mainly played regulatory role in mixed liquor.
Embodiment two
A kind of composite bacterial, including elongated bacillus, oblong bacillus, yeast and Bacillus acidi lactici, by quality
Percentage calculates, elongated bacillus：Oblong bacillus：Yeast：Bacillus acidi lactici=1:1:1.5:0.4.
Elongated bacillus size is 1.8um × 4.6um.
Oblong bacillus size is 0.4um × 0.8um.
The preparation method of composite bacterial：Comprise the following steps：
<1>The preparation of bacillus
With beef-protein medium, PH is in 7.2-7.4, first autoclaving 30 minutes, then inoculated and cultured, after inoculation
30 degree of constant temperature is cultivated 48 hours, and the preparation of bacillus is completed；
<2>The preparation of saccharomycete
Saccharomycete is individually cultivated 24 hours, prepared by saccharomycete completes；
<3>The preparation of lactic acid bacteria
Lactic acid bacteria is individually cultivated 24 hours, prepared by lactic acid bacteria completes；
<4>Mixed culture
The bacillus completed, saccharomycete, lactic acid bacteria these three bacterium will be prepared to be mixed 48 hours, prepared by composite bacterial
Complete.
Using the method for above-mentioned composite bacterial degraded animal waste, comprise the following steps：(1) it is above-mentioned composite bacterial 25 is public
Jin is sprayed on every cubic metre of animal waste；(2) after having sprayed, animal waste and mixed liquor are well mixed；(3) will mix equal
Animal waste after even carries out heap fermentation；(4) after fermenting 5 days, animal waste can be decomposed.
A kind of composite bacterial, bacterial content is at 3,000 ten thousand every milliliter.
The embodiment has the advantages that：(1) this compound bacteria, can be in the short time after spraying application on animal waste
It can bottom out its ammonia taste, 3-4 hours, temperature can rise to 60 degree or so within 12 hours, 3-5 just without the stink of excrement
It can complete fermentation completing technology effect；(2) excrement after this fermentation, can make excrement quick composting, harmful thing
Matter changes into the utilizable organic nutrition of plant, will not produce the side effects such as burn to any plant；(3) lactic acid bacteria is this
Mainly played regulatory role in mixed liquor.
Embodiment three
A kind of composite bacterial, including elongated bacillus, oblong bacillus, yeast and Bacillus acidi lactici, by quality
Percentage calculates, elongated bacillus：Oblong bacillus：Yeast：Bacillus acidi lactici=1:1:1.5:0.4.
Elongated bacillus size is 1.6um × 4.0um.
Oblong bacillus size is 0.6um × 1.2um.
The preparation method of composite bacterial：Comprise the following steps：
<1>The preparation of bacillus
With beef-protein medium, PH is in 7.2-7.4, first autoclaving 30 minutes, then inoculated and cultured, after inoculation
38 degree of constant temperature is cultivated 48 hours, and the preparation of bacillus is completed；
<2>The preparation of saccharomycete
Saccharomycete is individually cultivated 24 hours, prepared by saccharomycete completes；
<3>The preparation of lactic acid bacteria
Lactic acid bacteria is individually cultivated 38 hours, prepared by lactic acid bacteria completes；
<4>Mixed culture
The bacillus completed, saccharomycete, lactic acid bacteria these three bacterium will be prepared to be mixed 60 hours, prepared by composite bacterial
Complete.
Using the method for above-mentioned composite bacterial degraded animal waste, comprise the following steps：(1) it is above-mentioned composite bacterial 18 is public
Jin is sprayed on every cubic metre of animal waste；(2) after having sprayed, animal waste and composite bacterial are well mixed；(3) will mixing
Animal waste after uniformly carries out heap fermentation；(4) after fermenting 4 days, animal waste can be decomposed.
Composite bacterial, bacterial content is at 5,000 ten thousand every milliliter.
The embodiment has the advantages that：(1) this compound bacteria, can be in the short time after spraying application on animal waste
It can bottom out its ammonia taste, 3-4 hours, temperature can rise to 60 degree or so within 12 hours, 3-5 just without the stink of excrement
It can complete fermentation completing technology effect；(2) excrement after this fermentation, can make excrement quick composting, harmful thing
Matter changes into the utilizable organic nutrition of plant, will not produce the side effects such as burn to any plant；(3) lactic acid bacteria is this
Mainly played regulatory role in mixed liquor.
Above example is only the exemplary embodiment of the present invention, is not used in the limitation present invention, those skilled in the art can
With within the scope of the present invention, various modifications or equivalent substitution are made to the present invention, this modification or equivalent substitution also should
It is considered as and is within the scope of the present invention.
Claims (8)
- A kind of 1. composite bacterial, it is characterised in that：Including elongated bacillus, oblong bacillus, yeast and lactic acid bar Bacterium, calculate by mass percentage, elongated bacillus：Oblong bacillus：Yeast：Bacillus acidi lactici=1:1:1.5:0.4.
- 2. composite bacterial as claimed in claim 1, it is characterised in that：Described elongated bacillus size is 1.4-1.8um ×3.5-4.6um。
- 3. composite bacterial as claimed in claim 1, it is characterised in that：Described oblong bacillus size is 0.4- 0.9um×0.8-1.5um。
- 4. the preparation method of composite bacterial as claimed in claim 1：Comprise the following steps：<1>The preparation of bacillusWith beef-protein medium, PH is in 7.2-7.4, first autoclaving 30 minutes, then inoculated and cultured, constant temperature after inoculation 30-38 degree culture 48 hours, the preparation of bacillus are completed；<2>The preparation of saccharomyceteSaccharomycete is individually cultivated 24 hours, prepared by saccharomycete completes；<3>The preparation of lactic acid bacteriaLactic acid bacteria is individually cultivated 24-38 hours, prepared by lactic acid bacteria completes；<4>Mixed cultureThe bacillus completed, saccharomycete, lactic acid bacteria these three bacterium mixed culture 48-60 hours will be prepared, prepared by composite bacterial Complete.
- A kind of 5. method for animal waste of degrading, it is characterised in that：Comprise the following steps：(1) will answer as claimed in claim 1 10-25 kilograms of bacterium is closed to be sprayed on every cubic metre of animal waste；(2) after having sprayed, animal waste and composite bacterial are mixed Uniformly；(3) animal waste after will be well mixed carries out heap fermentation；(4) after fermenting 3-5 days, animal waste can be decomposed.
- 6. the method for degraded animal waste as claimed in claim 5, it is characterised in that：Described composite bacterial, bacterial content Every milliliter more than 2,000 ten thousand.
- 7. the method for degraded animal waste as claimed in claim 5, it is characterised in that：Described elongated bacillus size is 1.4-1.8um×3.5-4.6um。
- 8. the method for degraded animal waste as claimed in claim 5, it is characterised in that：Described oblong bacillus is big Small is 0.4-0.9um × 0.8-1.5um.
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