CN107189972A - Microbial deodorant, preparation method and the usage - Google Patents
Microbial deodorant, preparation method and the usage Download PDFInfo
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Abstract
The present invention provides a kind of microbial deodorant, preparation method and the usage, and microbial deodorant includes bacillus, saccharomycete and lactic acid bacteria.Microbial deodorant is used for all kinds of house refuses and cultivation fecal deodorizing, and its deodorisation process is：Microbial deodorant is put into house refuse and excrement, the saccharomycete in microbial bacterial agent can secretion activity material, promote beneficial microbe growth, itself can produce fragrance；Lactic acid bacteria can carry out organic matter fermentation, adjust pH, suppress harmful bacteria growing；Bacillus can produce enzyme, suppress the release of foul gas；When microbial deodorant is sprayed on into house refuse and excrement surface, because microbial deodorant is presented acid, it is possible to decrease the concentration of ammonia near house refuse and excrement, deodorizing effect is reached.With advantages below：The microbial deodorant that the present invention is provided, is a kind of pollution-free, efficient, safe microbial deodorant, and it is not thorough to solve chemical deodorizing agent deodorization, the problem of pollution environment.
Description
Technical field
The invention belongs to technical field of environmental microorganism, and in particular to a kind of microbial deodorant, its preparation method and use
On the way.
Background technology
With expanding economy, growth in the living standard, municipal refuse yield is also greatly improved, and the rubbish produced every year reaches
To 200,000,000 tons, volume of cargo in storage reaches 6,600,000,000 tons.These rubbish produce substantial amounts of stench, strong stimulation, inflammable and explosive gas.With ring
The enhancing of consciousness is protected, people increasingly focus on influence of the foul gas to environment, and it is separated from atmosphere pollution
Separately as one of seven big public hazards.
Stench be peculiar smell in the general name of various peculiar smell, big gas and water, discarded object by air dielectric, act on smelling for people
Feel thinking and be perceived.According to domestic and international relevant discussion, stench can be defined as：Every energy loss evil human habitat, generation
Make us insufferable smell or make one to produce the gas common name stench of unhappy sensation.
In the prior art, it is main that deficiency below deodorization, generally existing is carried out using chemical deodorizing agent：(1) chemical deodorizing is produced
Product are to lean in chemical reagent to add essence, and simply stink is covered, cured the symptoms, not the disease, stink substance is not eliminated from source
Generation.(2) chemical raw material that chemical deodorizing agent is used, has certain toxicity to human body and environment.
The content of the invention
The defect existed for prior art, the present invention provides a kind of microbial deodorant, preparation method and the usage, can
Effectively solve the above problems.
The technical solution adopted by the present invention is as follows：
The present invention provides a kind of microbial deodorant, including bacillus, saccharomycete and lactic acid bacteria.
It is preferred that, the weight proportion of each component is：
The parts by weight of bacillus 20
The parts by weight of saccharomycete 30~50
The parts by weight of lactic acid bacteria 40~50.
It is preferred that, the weight proportion of each component is：
The parts by weight of bacillus 20
The parts by weight of saccharomycete 40
The parts by weight of lactic acid bacteria 45.
It is preferred that, total bacteria count in microbial deodorant>109CFU/mL。
The present invention also provides a kind of preparation method of microbial deodorant, comprises the following steps：
Step 1, saccharomycete slant medium, lactic acid bacteria slant medium, bacillus inclined-plane culture are prepared respectively
Base, saccharomycete fluid nutrient medium, lactic acid bacteria fluid nutrient medium and bacillus liquid culture medium；
Step 2, saccharomycete is inoculated in saccharomycete slant medium, in after activation 20h under conditions of 35 DEG C, activated
Saccharomycete afterwards；
By lactobacillus inoculum in lactic acid bacteria slant medium, activated under conditions of 35 DEG C after 18h, the breast after being activated
Sour bacterium；
Bacillus is inoculated in bacillus slant medium, in after activation 18h under conditions of 35 DEG C, obtained after activation
Bacillus；
Step 3, the saccharomycete after activation is inoculated in saccharomycete fluid nutrient medium, 30 DEG C culture 24h, until seed into
It is ripe, prepare saccharomycete first order seed；
By the lactobacillus inoculum after activation in lactic acid bacteria fluid nutrient medium, 37 DEG C of culture 24h, until seed maturity, system
It is standby to obtain lactic acid bacteria first order seed；
Bacillus after activation is inoculated in bacillus liquid culture medium, 37 DEG C culture 24h, until seed into
It is ripe, prepare bacillus first order seed；
Step 4, saccharomycete first order seed, lactic acid bacteria first order seed and bacillus first order seed are inoculated into by formula ratio
Fermented in liquid fermentation tank culture medium；Wherein, fermentation is：30~35 DEG C of temperature, 300~400r/ of speed of agitator
Min, coefficient control is 85%, and pressure 0.1MPa ferments 3~5 days, prepares microbial deodorant crude product；
Step 5, essence is added into microbial deodorant crude product, strengthens deodorizing effect, obtains final microorganism and remove
Smelly dose.
It is preferred that, the saccharomycete inclined-plane culture based component is：The brown sugar of 4.0% mass fraction, 1.0% mass fraction
Corn steep liquor, the peptone of 0.5% mass fraction, the sodium chloride of 1.0% mass fraction, remaining is water；
The saccharomycete liquid medium component is：The molasses of 8.0% mass fraction, the corn steep liquor of 1.0% mass fraction,
The ammonium sulfate of 0.5% mass fraction, the potassium dihydrogen phosphate of 0.2% mass fraction, the magnesium sulfate of 0.1% mass fraction, 0.1% matter
The sodium chloride of fraction is measured, remaining is water；PH 6.5, tank pressure 0.07~0.08MPa, rotating speed 130r/min；
The composition of the lactic acid bacteria slant medium is：The peptone of 0.5% mass fraction, the jade of 2.0% mass fraction
Rice ＆ peanut milk, the sucrose of 4.0% mass fraction, the sodium acetate of 0.5% mass fraction, the magnesium sulfate of 0.5% mass fraction, 1.0% matter
The sodium chloride of fraction is measured, remaining is water；
The composition of the lactic acid bacteria fluid nutrient medium is：The peptone of 1.0% mass fraction, the ox of 1.0% mass fraction
Meat extract, the diammonium hydrogen citrate of 0.2% mass fraction, the glucose of 2.0% mass fraction, the Tween 80 of 0.1% mass fraction,
The sodium acetate of 0.5% mass fraction, the dipotassium hydrogen phosphate of 0.2% mass fraction, the magnesium sulfate of 0.05% mass fraction,
The manganese sulfate of 0.025% mass fraction, remaining is water；pH 6.5；
The composition of the bacillus slant medium is：The peptone of 0.5% mass fraction, 1.0% mass fraction
Corn steep liquor, the sodium chloride of 1.0% mass fraction, remaining is water；
The composition of the bacillus liquid culture medium is：The cornstarch of 2.0% mass fraction, 1.0% mass fraction
Corn steep liquor, the ammonium sulfate of 0.5% mass fraction, the potassium dihydrogen phosphate of 0.2% mass fraction, the sulfuric acid of 0.1% mass fraction
Magnesium, the sodium chloride of 0.1% mass fraction, remaining is water；PH 6.5, tank pressure 0.07~0.08MPa, rotating speed 200r/min.
The present invention also provides a kind of purposes of microbial deodorant, and microbial deodorant is used for all kinds of house refuses and cultivation
Fecal deodorizing, its deodorisation process is：Microbial deodorant is put into house refuse and excrement, the yeast in microbial bacterial agent
Bacterium can secretion activity material, promote beneficial microbe growth, itself can produce fragrance；Lactic acid bacteria can carry out organic matter hair
Ferment, adjusts pH, suppresses harmful bacteria growing；Bacillus can produce enzyme, suppress the release of foul gas；By microbial deodorant
When agent is sprayed on house refuse and excrement surface, because microbial deodorant is presented acid, it is possible to decrease house refuse and excrement are attached
The concentration of nearly ammonia, reaches deodorizing effect.
There is good synergy between the microbial deodorant that the present invention is provided, contained each microbial bacteria.
Under the synergy, the effect of deodorization can be effectively realized.Specifically, the saccharomycete in microbial bacterial agent being capable of secretion activity thing
Matter, promotes beneficial microbe growth, itself can produce fragrance；Lactic acid bacteria can carry out organic matter fermentation, adjust pH, suppress to have
Evil bacteria growing；Bacillus can produce enzyme, suppress the release of foul gas；Microbial deodorant is sprayed on house refuse
And during excrement surface, because microbial deodorant is presented acid, it is possible to decrease the concentration of ammonia near house refuse and excrement, reach
Deodorizing effect.
Microbial deodorant that the present invention is provided, preparation method and the usage have advantages below：
The microbial deodorant that the present invention is provided, is a kind of pollution-free, efficient, safe microbial deodorant, solves
Chemical deodorizing agent deodorization is not thorough, the problem of polluting environment.
Brief description of the drawings
The flow chart for the microbial deodorant preparation method that Fig. 1 provides for the present invention.
Embodiment
In order that technical problem solved by the invention, technical scheme and beneficial effect are more clearly understood, below in conjunction with
Drawings and Examples, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to
The present invention is explained, is not intended to limit the present invention.
The present invention provides total bacteria count in a kind of microbial deodorant, microbial deodorant>109CFU/mL.The present invention is provided
Microbial deodorant, altogether comprising three kinds of microbial bacterias, be respectively：Saccharomycete, lactic acid bacteria and bacillus.Contained is each micro-
There is good synergy between biological bacteria.Under the synergy, the effect of deodorization can be effectively realized.Specifically, micro- life
Saccharomycete in thing microbial inoculum can secretion activity material, promote beneficial microbe growth, itself can produce fragrance；Lactic acid bacteria energy
Organic matter fermentation is enough carried out, pH is adjusted, suppresses harmful bacteria growing；Bacillus can produce enzyme, suppress releasing for foul gas
Put；When microbial deodorant is sprayed on into house refuse and excrement surface, because microbial deodorant is presented acid, it is possible to decrease raw
The concentration of ammonia, reaches deodorizing effect near rubbish and excrement living.
The microbial deodorant of the present invention, deodorizing capability is strong, efficiency high, is adapted to house refuse, plant's animal wastes, dirt
The deodorization of all kinds of materials such as mud.
The microbial deodorant of the present invention, including bacillus, saccharomycete and lactic acid bacteria.The weight proportion of each component is：
The parts by weight of bacillus 20；The parts by weight of saccharomycete 30~50；The parts by weight of lactic acid bacteria 40~50.Total bacteria count in microbial deodorant>
109CFU/mL。
The preparation method of microbial deodorant, comprises the following steps：
Step 1, saccharomycete slant medium, lactic acid bacteria slant medium, bacillus inclined-plane culture are prepared respectively
Base, saccharomycete fluid nutrient medium, lactic acid bacteria fluid nutrient medium and bacillus liquid culture medium；
Wherein, the saccharomycete inclined-plane culture based component is：The brown sugar of 4.0% mass fraction, the jade of 1.0% mass fraction
Rice ＆ peanut milk, the peptone of 0.5% mass fraction, the sodium chloride of 1.0% mass fraction, remaining is water；
The saccharomycete liquid medium component is：The molasses of 8.0% mass fraction, the corn steep liquor of 1.0% mass fraction,
The ammonium sulfate of 0.5% mass fraction, the potassium dihydrogen phosphate of 0.2% mass fraction, the magnesium sulfate of 0.1% mass fraction, 0.1% matter
The sodium chloride of fraction is measured, remaining is water；PH 6.5, tank pressure 0.07~0.08MPa, rotating speed 130r/min；
The composition of the lactic acid bacteria slant medium is：The peptone of 0.5% mass fraction, the jade of 2.0% mass fraction
Rice ＆ peanut milk, the sucrose of 4.0% mass fraction, the sodium acetate of 0.5% mass fraction, the magnesium sulfate of 0.5% mass fraction, 1.0% matter
The sodium chloride of fraction is measured, remaining is water；
The composition of the lactic acid bacteria fluid nutrient medium is：The peptone of 1.0% mass fraction, the ox of 1.0% mass fraction
Meat extract, the diammonium hydrogen citrate of 0.2% mass fraction, the glucose of 2.0% mass fraction, the Tween 80 of 0.1% mass fraction,
The sodium acetate of 0.5% mass fraction, the dipotassium hydrogen phosphate of 0.2% mass fraction, the magnesium sulfate of 0.05% mass fraction,
The manganese sulfate of 0.025% mass fraction, remaining is water；pH 6.5；
The composition of the bacillus slant medium is：The peptone of 0.5% mass fraction, 1.0% mass fraction
Corn steep liquor, the sodium chloride of 1.0% mass fraction, remaining is water；
The composition of the bacillus liquid culture medium is：The cornstarch of 2.0% mass fraction, 1.0% mass fraction
Corn steep liquor, the ammonium sulfate of 0.5% mass fraction, the potassium dihydrogen phosphate of 0.2% mass fraction, the sulfuric acid of 0.1% mass fraction
Magnesium, the sodium chloride of 0.1% mass fraction, remaining is water；PH 6.5, tank pressure 0.07~0.08MPa, rotating speed 200r/min.
Step 2, saccharomycete is inoculated in saccharomycete slant medium, in after activation 20h under conditions of 35 DEG C, activated
Saccharomycete afterwards；
By lactobacillus inoculum in lactic acid bacteria slant medium, activated under conditions of 35 DEG C after 18h, the breast after being activated
Sour bacterium；
Bacillus is inoculated in bacillus slant medium, in after activation 18h under conditions of 35 DEG C, obtained after activation
Bacillus；
Step 3, the saccharomycete after activation is inoculated in saccharomycete fluid nutrient medium, 30 DEG C culture 24h, until seed into
It is ripe, prepare saccharomycete first order seed；
By the lactobacillus inoculum after activation in lactic acid bacteria fluid nutrient medium, 37 DEG C of culture 24h, until seed maturity, system
It is standby to obtain lactic acid bacteria first order seed；
Bacillus after activation is inoculated in bacillus liquid culture medium, 37 DEG C culture 24h, until seed into
It is ripe, prepare bacillus first order seed；
Step 4, saccharomycete first order seed, lactic acid bacteria first order seed and bacillus first order seed are inoculated into by formula ratio
Fermented in liquid fermentation tank culture medium；Wherein, fermentation is：30~35 DEG C of temperature, 300~400rpm of speed of agitator,
Coefficient control is 85%, and pressure 0.1MPa ferments 3~5 days, prepares microbial deodorant crude product；
Step 5, essence is added into microbial deodorant crude product, strengthens deodorizing effect, obtains final microorganism and remove
Smelly dose.
The present invention is further elaborated by the following examples
Following 8 kinds of culture mediums, saccharomycete slant medium, lactic acid bacteria slant medium, bacillus are prepared respectively
Slant medium, saccharomycete fluid nutrient medium, lactic acid bacteria fluid nutrient medium and bacillus liquid culture medium, it is standby.
Wherein, the saccharomycete inclined-plane culture based component is：The brown sugar of 4.0% mass fraction, the jade of 1.0% mass fraction
Rice ＆ peanut milk, the peptone of 0.5% mass fraction, the sodium chloride of 1.0% mass fraction, remaining is water；
The saccharomycete liquid medium component is：The molasses of 8.0% mass fraction, the corn steep liquor of 1.0% mass fraction,
The ammonium sulfate of 0.5% mass fraction, the potassium dihydrogen phosphate of 0.2% mass fraction, the magnesium sulfate of 0.1% mass fraction, 0.1% matter
The sodium chloride of fraction is measured, remaining is water；PH 6.5, tank pressure 0.07~0.08MPa, rotating speed 130r/min；
The composition of the lactic acid bacteria slant medium is：The peptone of 0.5% mass fraction, the jade of 2.0% mass fraction
Rice ＆ peanut milk, the sucrose of 4.0% mass fraction, the sodium acetate of 0.5% mass fraction, the magnesium sulfate of 0.5% mass fraction, 1.0% matter
The sodium chloride of fraction is measured, remaining is water；
The composition of the lactic acid bacteria fluid nutrient medium is：The peptone of 1.0% mass fraction, the ox of 1.0% mass fraction
Meat extract, the diammonium hydrogen citrate of 0.2% mass fraction, the glucose of 2.0% mass fraction, the Tween 80 of 0.1% mass fraction,
The sodium acetate of 0.5% mass fraction, the dipotassium hydrogen phosphate of 0.2% mass fraction, the magnesium sulfate of 0.05% mass fraction,
The manganese sulfate of 0.025% mass fraction, remaining is water；pH 6.5；
The composition of the bacillus slant medium is：The peptone of 0.5% mass fraction, 1.0% mass fraction
Corn steep liquor, the sodium chloride of 1.0% mass fraction, remaining is water；
The composition of the bacillus liquid culture medium is：The cornstarch of 2.0% mass fraction, 1.0% mass fraction
Corn steep liquor, the ammonium sulfate of 0.5% mass fraction, the potassium dihydrogen phosphate of 0.2% mass fraction, the sulfuric acid of 0.1% mass fraction
Magnesium, the sodium chloride of 0.1% mass fraction, remaining is water；PH 6.5, tank pressure 0.07~0.08MPa, rotating speed 200r/min.
Embodiment 1
Step 1, saccharomycete is inoculated in saccharomycete slant medium, in after activation 20h under conditions of 35 DEG C, activated
Saccharomycete afterwards；
By lactobacillus inoculum in lactic acid bacteria slant medium, activated under conditions of 35 DEG C after 18h, the breast after being activated
Sour bacterium；
Bacillus is inoculated in bacillus slant medium, in after activation 18h under conditions of 35 DEG C, obtained after activation
Bacillus；
Step 2, the saccharomycete after activation is inoculated in saccharomycete fluid nutrient medium, 30 DEG C culture 24h, until seed into
It is ripe, prepare saccharomycete first order seed；
By the lactobacillus inoculum after activation in lactic acid bacteria fluid nutrient medium, 37 DEG C of culture 24h, until seed maturity, system
It is standby to obtain lactic acid bacteria first order seed；
Bacillus after activation is inoculated in bacillus liquid culture medium, 37 DEG C culture 24h, until seed into
It is ripe, prepare bacillus first order seed；
Step 3, saccharomycete first order seed, lactic acid bacteria first order seed and bacillus first order seed are inoculated into by formula ratio
Fermented in liquid fermentation tank culture medium；Wherein, fermentation is：30 DEG C of temperature, speed of agitator 300r/min, coefficient
Control as 85%, pressure 0.1MPa ferments 3 days, prepares microbial deodorant crude product；
Step 4, essence is added into microbial deodorant crude product, strengthens deodorizing effect, obtains final microorganism and remove
Smelly dose.
Embodiment 2
Step 1, saccharomycete is inoculated in saccharomycete slant medium, in after activation 20h under conditions of 35 DEG C, activated
Saccharomycete afterwards；
By lactobacillus inoculum in lactic acid bacteria slant medium, activated under conditions of 35 DEG C after 18h, the breast after being activated
Sour bacterium；
Bacillus is inoculated in bacillus slant medium, in after activation 18h under conditions of 35 DEG C, obtained after activation
Bacillus；
Step 2, the saccharomycete after activation is inoculated in saccharomycete fluid nutrient medium, 30 DEG C culture 24h, until seed into
It is ripe, prepare saccharomycete first order seed；
By the lactobacillus inoculum after activation in lactic acid bacteria fluid nutrient medium, 37 DEG C of culture 24h, until seed maturity, system
It is standby to obtain lactic acid bacteria first order seed；
Bacillus after activation is inoculated in bacillus liquid culture medium, 37 DEG C culture 24h, until seed into
It is ripe, prepare bacillus first order seed；
Step 3, saccharomycete first order seed, lactic acid bacteria first order seed and bacillus first order seed are inoculated into by formula ratio
Fermented in liquid fermentation tank culture medium；Wherein, fermentation is：35 DEG C of temperature, speed of agitator 400r/min, coefficient
Control as 85%, pressure 0.1MPa ferments 5 days, prepares microbial deodorant crude product；
Step 4, essence is added into microbial deodorant crude product, strengthens deodorizing effect, obtains final microorganism and remove
Smelly dose.
Inspection example 1
This inspection example is used for the deodorizing capability for investigating microbial bacterial agent.
By microbial deodorant made from embodiments of the invention 1,10 times are diluted with deionized water, is mixed 10 minutes, system
Into deodorization bacterium solution：Chicken manure is taken from plant at random, 10 kilograms are respectively taken, two chambers is put into, micro- life was sprayed every 12 hours
Ammonia and concentration of hydrogen sulfide are detected after thing microbial inoculum, 48h.
Test result indicates that, microbial bacterial agent after spraying, because microbial deodorant is acidity, can be caught in air rapidly
Ammonia, reduce stink.
After testing, ammonia concentration declines 75% after 48h, and concentration of hydrogen sulfide declines 50%, hence it is evident that inhibit the generation of stink.
Inspection example 2
This inspection example is used for the deodorizing capability for investigating microbial bacterial agent.
Implement live deodorizing test in refuse landfill, meteorological condition is below 25 degree of temperature, 3 grades of wind speed.In operation area
A sampled point is laid at domain center, and the microbial deodorant 100ml prepared by one square metre of sprinkling embodiment two, sprinkling is real
Shi Hou, 10 minutes and 40 minutes detection hydrogen sulfide and ammonia rate of descent, so as to detect deodorizing effect.
As can be seen here, the microbial deodorant that uses of the present invention, when carrying out deodorization to house refuse, with instant effect and
Deodorizing effect clear advantage.
Inspection example 3
Corrupt house refuse and chicken manure are taken, according to 1:1 ratio mixing, then by mixture and water according to 1：1.5 mixing
Filtered after uniform, collect filtrate and used to do source of odor.
Source of odor solution is added into 10ml into each triangular flask, each strain 10ml is then respectively connected to, 30 DEG C,
The deodorizing capability for detecting each bacterium after 48h with odor threshold method is cultivated under conditions of 150rpm.
Experimental method：1ml zymotic fluids are taken in small beaker, deionization is then added with each 10ml amount into small beaker
Water, until can't smell stink, records diluted multiple, the preliminary judgment criteria screened as deodorization bacterium.Experimental result is as follows
Shown in table.
In upper table, saccharomycete, lactic acid bacteria and the bacillus tested are the correspondence one that the step 2 of embodiment two is obtained
Level seed.And 3 kinds of bacterium are mixed into the microbial deodorant that embodiment two is finally prepared.
Degraded of saccharomycete, lactic acid bacteria and the bacillus screened it can be seen from data above to stink substance
Rate is all below 80%, and 3 kinds of bacterium are up to 95.2% after the mixing of order of magnitude equal percentage to the clearance of stink substance, thus
Fully demonstrate after 3 kinds of bacterium mixing, synergy is mutually played, so as to improve deodorizing effect.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
Depending on protection scope of the present invention.
Claims (7)
1. a kind of microbial deodorant, it is characterised in that including bacillus, saccharomycete and lactic acid bacteria.
2. microbial deodorant according to claim 1, it is characterised in that the weight proportion of each component is：
The parts by weight of bacillus 20
The parts by weight of saccharomycete 30~50
The parts by weight of lactic acid bacteria 40~50.
3. microbial deodorant according to claim 2, it is characterised in that the weight proportion of each component is：
The parts by weight of bacillus 20
The parts by weight of saccharomycete 40
The parts by weight of lactic acid bacteria 45.
4. microbial deodorant according to claim 1, it is characterised in that total bacteria count in microbial deodorant>109CFU/
mL。
5. a kind of preparation method of microbial deodorant, it is characterised in that comprise the following steps：
Step 1, saccharomycete slant medium, lactic acid bacteria slant medium, bacillus slant medium, ferment are prepared respectively
Female bacteria liquid culture medium, lactic acid bacteria fluid nutrient medium and bacillus liquid culture medium；
Step 2, saccharomycete is inoculated in saccharomycete slant medium, activated under conditions of 35 DEG C after 20h, after being activated
Saccharomycete；
By lactobacillus inoculum in lactic acid bacteria slant medium, activated under conditions of 35 DEG C after 18h, the lactic acid after being activated
Bacterium；
Bacillus is inoculated in bacillus slant medium, activated under conditions of 35 DEG C after 18h, the bud after being activated
Spore bacillus；
Step 3, the saccharomycete after activation is inoculated in saccharomycete fluid nutrient medium, 30 DEG C of culture 24h, until seed maturity,
Prepare saccharomycete first order seed；
By the lactobacillus inoculum after activation in lactic acid bacteria fluid nutrient medium, 37 DEG C of culture 24h, until seed maturity, are prepared into
To lactic acid bacteria first order seed；
Bacillus after activation is inoculated in bacillus liquid culture medium, 37 DEG C of culture 24h, until seed maturity, system
It is standby to obtain bacillus first order seed；
Step 4, saccharomycete first order seed, lactic acid bacteria first order seed and bacillus first order seed are inoculated into liquid by formula ratio
Fermented in fermentation tank culture medium；Wherein, fermentation is：30~35 DEG C of temperature, 300~400rpm of speed of agitator, charging
Coefficient control is 85%, and pressure 0.1MPa ferments 3~5 days, prepares microbial deodorant crude product；
Step 5, essence is added into microbial deodorant crude product, strengthens deodorizing effect, obtains final microbial deodorant.
6. the preparation method of microbial deodorant according to claim 5, it is characterised in that the saccharomycete inclined-plane culture
Based component is：The brown sugar of 4.0% mass fraction, the corn steep liquor of 1.0% mass fraction, the peptone of 0.5% mass fraction,
The sodium chloride of 1.0% mass fraction, remaining is water；
The saccharomycete liquid medium component is：The molasses of 8.0% mass fraction, the corn steep liquor of 1.0% mass fraction,
The ammonium sulfate of 0.5% mass fraction, the potassium dihydrogen phosphate of 0.2% mass fraction, the magnesium sulfate of 0.1% mass fraction, 0.1% matter
The sodium chloride of fraction is measured, remaining is water；PH 6.5, tank pressure 0.07~0.08MPa, rotating speed 130r/min；
The composition of the lactic acid bacteria slant medium is：The peptone of 0.5% mass fraction, the corn steep liquor of 2.0% mass fraction,
The sucrose of 4.0% mass fraction, the sodium acetate of 0.5% mass fraction, the magnesium sulfate of 0.5% mass fraction, 1.0% mass fraction
Sodium chloride, remaining is water；
The composition of the lactic acid bacteria fluid nutrient medium is：The peptone of 1.0% mass fraction, the beef extract of 1.0% mass fraction,
The diammonium hydrogen citrate of 0.2% mass fraction, the glucose of 2.0% mass fraction, the Tween 80 of 0.1% mass fraction, 0.5%
The sodium acetate of mass fraction, the dipotassium hydrogen phosphate of 0.2% mass fraction, the magnesium sulfate of 0.05% mass fraction, 0.025% mass
The manganese sulfate of fraction, remaining is water；pH 6.5；
The composition of the bacillus slant medium is：The peptone of 0.5% mass fraction, the corn of 1.0% mass fraction
Slurry, the sodium chloride of 1.0% mass fraction, remaining is water；
The composition of the bacillus liquid culture medium is：The cornstarch of 2.0% mass fraction, the jade of 1.0% mass fraction
Rice ＆ peanut milk, the ammonium sulfate of 0.5% mass fraction, the potassium dihydrogen phosphate of 0.2% mass fraction, the magnesium sulfate of 0.1% mass fraction,
The sodium chloride of 0.1% mass fraction, remaining is water；PH 6.5, tank pressure 0.07~0.08MPa, rotating speed 200r/min.
7. a kind of purposes of microbial deodorant, it is characterised in that microbial deodorant is used for all kinds of house refuses and cultivation excrement
Just deodorization, its deodorisation process is：Microbial deodorant is put into house refuse and excrement, the saccharomycete in microbial bacterial agent
Can secretion activity material, promote beneficial microbe growth, itself can produce fragrance；Lactic acid bacteria can carry out organic matter hair
Ferment, adjusts pH, suppresses harmful bacteria growing；Bacillus can produce enzyme, suppress the release of foul gas；By microbial deodorant
When agent is sprayed on house refuse and excrement surface, because microbial deodorant is presented acid, it is possible to decrease house refuse and excrement are attached
The concentration of nearly ammonia, reaches deodorizing effect.
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