CN110343649A - A kind of microbial deoderizer and its application - Google Patents
A kind of microbial deoderizer and its application Download PDFInfo
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- CN110343649A CN110343649A CN201910785063.5A CN201910785063A CN110343649A CN 110343649 A CN110343649 A CN 110343649A CN 201910785063 A CN201910785063 A CN 201910785063A CN 110343649 A CN110343649 A CN 110343649A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/30—Sulfur compounds
- B01D2257/306—Organic sulfur compounds, e.g. mercaptans
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The present invention provides a kind of microbial deoderizer and its application, is related to microbial bacterial agent technical field.The microbial deoderizer, it is characterized in that, the microbial deoderizer includes at least bacillus, saccharomycete, lactic acid bacteria, above-mentioned strain passes through respectively individually to be cultivated, it is cultivated in big tank in combination access according to a certain percentage, cultivation temperature and speed of agitator are controlled, the microbial deoderizer is prepared within culture 3 days.The microbial deoderizer prepared is applied to livestock and poultry farm or organic fertilizer production factory or garbage reclamation treating stations carry out deodorization.
Description
Technical field
The invention belongs to microbial bacterial agent technical fields, more particularly to a kind of microbial deoderizer and its application.
Background technique
In recent years, China's economy is always maintained at the developing state of high speed, and the following environmental problem but highlights seriously, greatly
Gas pollution, water pollution, solid waste refuse pollution etc. are seen everywhere.With the continuous appearance of environmental protection policy and national environmental consciousness
With the continuous reinforcement of theory, environment protection treating has entered the stage that manufacturing enterprise must reform.Wherein foul smell discharge is all conglomeraties
The pollution category faced, technical method used by enterprise's odor treatment are that foul smell generates workshop and passes through negative pressure gas collection
Pipeline is unified to enter series spray deodorizing device, finally realizes qualified discharge.Wherein spray column device configuration dosing cyclic water tank,
Deodorant can be added in water tank, realized absorption and resolution process to stink odor, mitigated the processing pressure of follow-up link.Deodorization
Agent is broadly divided into chemical Strong oxdiative medicament or flavouring agent, plant extract deodorant and microbial deodorant three types, wherein micro-
Biological deodorant most ecological, environmental protective, deodorizing effect are also relatively good.Microbial deoderizer prepared by the present invention is mainly used in
It is smelly can quickly to remove ammonia, hydrogen sulfide, volatility lipid etc. for scale livestock farming, Biohazard Waste Disposal, organic fertilizer processing enterprise etc.
Gas composition.
Summary of the invention
The purpose of the present invention is to provide a kind of microbial deoderizers, can be to scale livestock farming, garbage disposal
It stands, the foul smell such as ammonia, hydrogen sulfide, volatile fatty acid of the generations such as organic fertilizer processing enterprise quickly remove, and purify air.
It is a further object of the present invention to provide the application of the microbial deoderizer of preparation, using space is spraying or spray column
Mode be applied to livestock and poultry farm or organic fertilizer production factory or garbage reclamation treating stations carry out deodorization.
Particular content of the present invention are as follows:
The microbial deoderizer includes at least bacillus, saccharomycete, lactic acid bacteria;Above-mentioned pass through respectively is individually cultivated, and is pressing
It is cultivated in big tank according to certain ratio combination access, controls cultivation temperature and speed of agitator, be prepared within culture 3 days described micro-
Biological deodorizing microbial inoculum.
Wherein the bacillus is bacillus subtilis, bacillus amyloliquefaciens, bacillus coagulans, lichens gemma bar
One or more kinds of combinations of bacterium;The saccharomycete is saccharomyces cerevisiae;The lactic acid bacteria be lactobacillus plantarum, lactobacillus acidophilus,
Lactobacillus casei, enterococcus faecium, one or more kinds of combinations of Lactobacillus rhamnosus.
Big tank culture medium prescription -1L: peptone 2-5g, beef extract 1-3g, yeast extract 1.5-3g, starch 2-5g, glucose
3-8g, sodium acetate 1-3g, potassium dihydrogen phosphate 0.5g, sodium chloride 0.8-3g, magnesium sulfate 0.3g, microelement 1ml, glycerol 1ml.
Preparation step is as follows: activated spawn, sterile liquid culture, bacillus and saccharomycete are distinguished first from strain library
In LB culture medium, lactic acid bacteria is cultivated respectively in MRS culture medium.
Bacillus and the saccharomycete 50-220rpm on shaking table, 30-40 degree culture, 24 hours or so, each classification strain
It is forwarded in big tank according to the ratio of 0.2-1%;Lactic acid bacteria does not stir, 30-40 degree, and 24 hours or so, each classification lactic acid bacteria was pressed
It is forwarded in big tank according to the ratio of 0.2-1%.
LB culture medium prescription -1L: tryptone 10g, yeast extract 5g, sodium chloride 10g.
MRS culture medium prescription -1L: peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose
20g, Tween 80 1ml, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g.
PET bucket or 304 stainless steel metal tanks that big tank uses common 1-10 ton are produced, stirring motor and constant temperature are equipped with
Heating apparatus.
It is first carried out disinfection processing with liquor natrii hypochloritis before big tank culture production, then by the big tank culture medium of the sterilizing of concentration
It is added with the tap water through ultrafiltration membrance filter to tank body, dissolves Media Components, bacillus, ferment is added according to aforementioned proportion
Female bacterium, the combination of lactic acid bacteria, 30-40 degree, 50-100rpm are stirred at low speed, and are cultivated 3-4 days or so, that is, are prepared into the micro- life of liquid
Object deodorizing microorganism is counted by micro- sem observation peace plate to detect the number of viable of microorganism.
The liquid microbe deodorizing microorganism prepared is applied in scale livestock farming, Biohazard Waste Disposal, organic fertilizer processing enterprise
The odor purifications such as ammonia, hydrogen sulfide, the volatile fatty acid of the generations such as industry.According to deodorization site specific, by liquid deodorant
Microbial inoculum dilutes 20-100 times, and spraying or spray mode carries out deodorization.
Microbial bacterial agent detection method:
Culture medium flat plate coating count plate appropriate may be selected in the microbial inoculum that culture is completed.Take 1ml bacterium solution sterile to 9ml
10 times of dilution in water, i.e., 10-1Gradient, then above-mentioned dilution 1ml is taken to dilute 10 times, i.e., 10 again into 9ml sterile water-2Gradient, according to
Secondary method is diluted to 10-6, 10-7, 10-8After take 1ml coating culture, count respective flat-plate bacterial colony sum respectively, each sample does 3
It is a parallel, calculate average.Lactic acid bacteria uses above-mentioned MRS culture medium, and bacillus and saccharomycete select above-mentioned LB culture medium.
Main advantages of the present invention are:
1) compared with the preparation method of existing microbial deoderizer, the present invention is without using industrial fermentation tank equipment and steam
The tank that goes out can prepare high activity deodorizing microorganism microbial inoculum, and preparation method is relatively simple, easily operated.
2) liquid microbe deodorizing microorganism retention cycle prepared by the present invention is 10 months, and deodorizing effect is not by viable bacteria
The influence of quantity variation.
Specific embodiment
The preparation method and application in invention about microbial deoderizer is described further below with reference to example.It answers
Understand, these examples are only for illustrating the present invention and are not intended to limit the scope of the present invention, further includes each specific embodiment
Between any combination.
The method that example 1 prepares 100L microbial deoderizer, comprising the following steps:
Activated first generation bacillus subtilis and saccharomycete on solid plate are taken, is seeded to the level-one of 500ml sterilizing respectively
In LB seed culture medium, 200rpm, 30 degree of shaking tables are cultivated 24 hours.
Activated first generation lactobacillus plantarum on solid plate is taken, the level-one MRS seed culture of 500ml sterilizing is seeded to
It in base, stands, 30 degree, cultivates 24 hours.
The big tank culture medium (g/L) of 100L: peptone 3g, beef extract 3g, yeast extract 3g, starch 2g, glucose 5g, sodium acetate
2g, potassium dihydrogen phosphate 0.5g, sodium chloride 1g, magnesium sulfate 0.3g, microelement 1ml, glycerol 1ml.According to above-mentioned formula, by 100L
Culture medium needed for big tank adds water to be concentrated in a 5L bottle, 105 degree of high-temperature boiling 5min, is then added to sterilizing in advance
In the big tank of 100L, it is passed through the tap water of ultrafiltration membrance filter, by above-mentioned cultured bacillus subtilis, saccharomycete, plant cream bar
The respective whole 500ml of bacterium first order seed bacterium solution, i.e., be respectively forwarded in the big tank of 100L by 0.5% inoculum concentration, controls 30 degree of temperature,
After 50rpm stirs at low speed culture 3 days, number of viable is detected, bacillus subtilis and lactobacillus plantarum can reach 109cfu/
The order of magnitude of ml, saccharomycete 108The cfu/ml order of magnitude, and have apparent fermentation sour taste, pH value 4 or so is detected, that is, is prepared into
The microbial deoderizer of 100L.
The method that example 2 prepares 2 tons of microbial deoderizers, comprising the following steps:
Activated first generation bacillus amyloliquefaciens and saccharomycete on solid plate are taken, is seeded to the one of 4000ml sterilizing respectively
In grade LB seed culture medium, 200rpm, 30 degree of shaking tables are cultivated 24 hours.
Activated first generation enterococcus faecium on solid plate is taken, the level-one MRS seed culture of 4000ml sterilizing is seeded to
It in base, stands, 30 degree, cultivates 24 hours.
2 tons big tank culture medium (g/L): peptone 2g, beef extract 2g, yeast extract 2g, starch 3g, glucose 8g, sodium acetate
3g, potassium dihydrogen phosphate 0.5g, sodium chloride 1g, magnesium sulfate 0.3g, microelement 1ml, glycerol 1ml.
According to above-mentioned formula, needs culture medium that water is added to be concentrated in two 30L stainless steel agitator tanks in 2 tons big tank, be passed through steaming
It 100 degree of vapour, boils 5 minutes.Then it is added to the 2 tons big tank of disinfection, is passed through the tap water of ultrafiltration membrance filter, it will be above-mentioned
Cultured bacillus amyloliquefaciens, saccharomycete, each 4000ml of first order seed bacterium solution of enterococcus faecium respectively press 0.2% inoculum concentration
It is forwarded in 2 tons big tank, controls 30 degree of temperature, after 50rpm stirs at low speed culture 3 days, detect number of viable, solve starch gemma bar
Bacterium and enterococcus faecium can reach 109The order of magnitude of cfu/ml, saccharomycete 108Cfu/ml, and have apparent fermentation sour taste,
PH value 4 or so is detected, i.e. expansion scale is prepared into microbial deoderizer.
Deodorization application of 3 microbial deoderizer of example at garbage disposal center
The deodorization bacterium solution that examples detailed above prepares can be applicable to scale livestock farming, Biohazard Waste Disposal, organic fertilizer processing enterprise
Equal places carry out deodorization.Garbage transfer station carries out the experiment of product deodorizing effect at Shaoxing environmental sanitation one, which is equipped with PLC timing certainly
Dosing spraying apparatus is controlled, in atomization operation range refuse compaction equipment workshop and haulage vehicle is into mill portion space.By deodorization
Bacterium solution is added after being diluted with water 100 times to equipment medicament barrel, and spray time is arranged, and every 10 minutes 30 seconds spraying, and the morning 2 is small
When, 2 hours afternoons.In initial one hour, the odor concentration sense organ of rubbish is substantially reduced very much, is operated normally after a week,
In spraying operation workshop, without refuse odor taste.
In the large-scale kitchen garbage processing center in Changxing, equipped with space atomising device, liquid deodorant microbial inoculum is diluted 50
It is added after times to medicine-adding bin.After kitchen haulage vehicle enters the station and kitchen processing equipment i.e. by operation start when, carry out space
It is spraying, it is sprayed 30 seconds per minute, diluted deodorization bacterium solution can be uniformly dispersed in entire space.When working truck and equipment are transported
After turning, continue 30 minutes by spraying, effectively removing the stink that kitchen garbage is distributed.
Removal application of 4 microbial deoderizer of example to industrial ethyl mercaptan foul smell
The foul smell of industrial volatile organic matter collects gas frequently with workshop negative pressure, is drained to the continuous tower of multi-stage spray and subsequent place
Manage equipment centralized processing.Shandong chemical company, it is ethyl mercaptan that foul smell, which discharges main component, and workshop is equipped with level Four and continuously sprays
Tower.First stage tower is condensed using conventional soda lye wash, second level tower using DMF, and three-level tower is using 10 times of dilution of deodorization bacterium solution, and four
Grade tower is using the deodorization bacterium solution for diluting 20 times, and after continuous deodorizing process, the stink of exhaust emissions is substantially reduced.Due to work
The concentration that industry discharges ethyl mercaptan is relatively high, and deodorizing microorganism only has certain processing capacity, when entering the 4th day or so, needs again
The deodorizing microorganism in the buck and three level Four spray columns in first order spray column is replaced, is then carrying out lasting spray column deodorization
Operation, during whole service, the removal rate of ethyl mercaptan greatly mitigates the uncontrollable discharge of stink up to 80% or more.
Claims (9)
1. a kind of microbial deoderizer, which is characterized in that the microbial deoderizer includes at least bacillus, yeast
Bacterium, lactic acid bacteria, it is above-mentioned respectively by individually cultivating, it is cultivated in big tank in combination access according to a certain percentage, control culture temperature
The microbial deoderizer is prepared in culture 3 days in degree and speed of agitator.
2. a kind of microbial deoderizer according to claim 1, which is characterized in that the bacillus is withered grass gemma
One or more kinds of combinations of bacillus, bacillus amyloliquefaciens, bacillus coagulans and bacillus licheniformis;The saccharomycete is
Saccharomyces cerevisiae;The lactic acid bacteria is lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, enterococcus faecium and Lactobacillus rhamnosus
One or more kinds of combinations.
3. a kind of microbial deoderizer according to claim 1, which is characterized in that the big tank culture medium prescription -1L
For peptone 2-5g, beef extract 1-3g, yeast extract 1.5-3g, starch 2-5g, glucose 3-8g, sodium acetate 1-3g, biphosphate
Potassium 0.5g, sodium chloride 0.8-3g, magnesium sulfate 0.3g, microelement 1ml, glycerol 1ml.
4. a kind of microbial deoderizer according to claim 1, which is characterized in that respectively by bacillus, saccharomycete,
Lactic acid bacteria is individually cultivated, and every kind of bacterium is forwarded in the big tank of culture according to the inoculum concentration of 0.2-1% ratio respectively.
5. a kind of microbial deoderizer according to claim 1, which is characterized in that the bacillus and saccharomycete exist
LB culture medium, lactic acid bacteria are cultivated respectively in MRS culture medium.
6. a kind of microbial deoderizer according to claim 1, which is characterized in that bacillus, saccharomycete, lactic acid bacteria
The cultivation temperature individually cultivated is 30 to 40 DEG C, mixing speed 50rpm-200rpm.
7. a kind of microbial deoderizer according to claim 1, which is characterized in that right before big tank is cultivated in access
It cultivates big tank to be pre-processed, first be carried out disinfection processing with liquor natrii hypochloritis, then by the big tank culture medium of the sterilizing of concentration
It is added with the tap water through ultrafiltration membrance filter to tank body, dissolves big tank culture medium.
8. a kind of microbial deoderizer application method according to claim 1, which is characterized in that remove the microorganism
Smelly microbial inoculum is applied to livestock and poultry farm or organic fertilizer production factory or garbage reclamation processing using space by spraying or by the way of spray column
It stands and carries out deodorization.
9. a kind of microbial deoderizer application method according to claim 1, which is characterized in that remove the microorganism
Smelly microbial inoculum is carrying out deodorization after diluting 20-100 times.
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Cited By (5)
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CN111254096A (en) * | 2020-02-28 | 2020-06-09 | 山东睿智医药科技有限公司 | Method for improving indoor environmental sanitation |
CN111676150A (en) * | 2020-05-25 | 2020-09-18 | 扬州市海诚生物技术有限公司 | Efficient deodorant bacterium and application thereof |
CN111961628A (en) * | 2020-08-28 | 2020-11-20 | 湖南科美洁环保科技有限公司 | Preparation method and application method of microbial deodorant |
CN112499770A (en) * | 2020-10-29 | 2021-03-16 | 诸暨市洛宏环保科技有限公司 | Environment-friendly deodorization device and deodorization method for kitchen wastewater |
CN112790287A (en) * | 2020-12-08 | 2021-05-14 | 中国农业科学院饲料研究所 | Preparation technology and application of environment-friendly comprehensive deodorization biological feed for fattening pigs |
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Address after: 313103 Chengnan Industrial Park, Heping Town, Changxing County, Huzhou City, Zhejiang Province Applicant after: Zhejiang Zhongyi Biological Environmental Protection Technology Co.,Ltd. Address before: 313103 Chengnan Industrial Park, Heping Town, Changxing County, Huzhou City, Zhejiang Province Applicant before: Zhejiang Zhongyi Biological Environmental Protection Technology Co.,Ltd. |
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Application publication date: 20191018 |