CN106902631A - A kind of microbial deodorant and production technology - Google Patents
A kind of microbial deodorant and production technology Download PDFInfo
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Abstract
A kind of microbial deodorant of the present invention, is formed by the material combination of following weight proportion:0.2 0.5% yeast extract, 5 15% molasses, 0.2 0.5% inorganic salts, 2.5 5% monosodium glutamate desalination solution, 5 10% microbial deodorant stoste, 70 80% water, the microbial deodorant are prepared from using following processing step:1)Weigh;2)Dissolving is mixed;3)The treatment of ultra micro physics and chemistry;4)PH is adjusted;5)Inoculation;6)Fermentation.Low production cost of the present invention, fermentation period are short, easy to operate, non-secondary pollution, while also effectively recycling monosodium glutamate desalination solution.And the deodorant stoste for using for Chinese medicine in extract, be used in deodorant microorganism fungus kind can cooperative fermentation growth, can be bred with syntrophism, avoid the phenomenon that the microorganism fungus kind that point tank culture remixes occurs antagonism, it is ensured that the stability and deodorizing effect of microbial deodorant product.
Description
Technical field
The invention belongs to microorganism and environmental technology field, and in particular to a kind of microbial deodorant and production technology.
Background technology
Foul gas can be divided into five classes from its composition.One is sulfur-containing compound, two be containing nitrogen compound, three be halogen and
Its derivative, four is hydro carbons, and five is oxygen containing organic matter.These odorants, are mostly organic matter outside vulcanisation hydrogen and ammonia, are disliked
The smelly influence to people is many.It not only multiplies substantial amounts of pathophorous insect, bacteria breed, and directly leads to
Olfactory system is crossed, strong impulse effect is produced to respiratory system, nervous system, the circulatory system, internal system.Short time
Effect, engender a repugnance the symptoms such as sense, Nausea and vomiting, and prolonged stimulation can cause endocrinopathy, angiocardiopathy etc.
Serious symptom.
Odor treatment research is the most advanced with states such as Japan, Holland, Germany.At present, the minimizing technology for stink is main
Have:Chemical deodorizing method, physical deodorization method and biological odor removal method, chemical deodorizing agent are relatively costly, easily cause secondary pollution;Plant removes
Smelly dose of extraction process complexity, production cost is higher, therefore both are greatly limited during scale use.And it is micro-
Biological deodorizing has the incomparable superiority of conventional method, as treatment effeciency is high, non-secondary pollution, needed for equipment letter
Single, easy to operate, low-cost, management service convenience etc., there is the irreplaceable technical advantage of other like products, and market holds
Amount is very big, and its development potentiality and application prospect are quite extensive.
The general principle of microbial deodorant treatment foul smell is that the odorant in dissolving water is absorbed in using microorganism
Microorganism itself in vivo, a kind of process of its degraded is made by approach such as the oxidation of microorganism, reduction, fermentations.It is essentially divided into
Three processes:1. the course of dissolution of foul gas, i.e., be the mass transport process of liquid phase by gas phase transition;2. foul smell soluble in water leads to
The cell membrane and cell membrane for crossing microorganism are absorbed by the micro-organisms, and water insoluble foul smell is first attached to outside microbial body, by micro- life
The exoenzyme of thing secretion is decomposed into solable matter, then penetrates into cell;3. after foul smell enters cell, in vivo as nutrients
Matter is that Institute of Micro-biology decomposes, utilizes.Unazotized material is broken down into CO2And H2O, the odor pollutant of sulfur-bearing can be oxidized decomposition
Into S, SO3 2-、SO4 2-, nitrogenous odor pollutant is then broken down into NH4 +、NO2-、NO3-.Once the active group oxygen of odorant
Change, smell just disappears.Meanwhile, these microorganisms can produce inorganic acid again, and formation is unfavorable for the acidity of putrefactive microorganisms life
Environment, and the material of foul gas is produced when fundamentally degraded is decomposed.
Current microbial deodorant product or technology are more, and such as China Agricultural University's research is with saccharomycete, lactic acid bacteria, nitrogen-fixing bacteria
With the non-bacillus such as photosynthetic bacteria compounding function bacillus deodorization technology, Guangdong Microbes Inst research with yeast
Deodorization technology based on bacterium, and produce microbial deodorant using yellow plasm of soybean.And not on utilizing monosodium glutamate desalination
Liquid carries out fermentation and prepares microbial deodorant.Therefore, domestic blank has been filled up using monosodium glutamate desalination solution production microbial deodorant,
Its market also has broad prospects.
The content of the invention
For the problem that prior art is present, a kind of microbial deodorant and production are provided it is an object of the invention to design
Technique.
Described a kind of microbial deodorant, it is characterised in that formed by the material combination of following weight proportion:0.2-
0.5% yeast extract, the molasses of 5-15%, the inorganic salts of 0.2-0.5%, the monosodium glutamate desalination solution of 2.5-5%, the microorganism of 5-10% remove
Smelly dose of stoste, the water of 70-80%, the microbial deodorant are prepared from using following processing step:
1)Weigh:Each raw material is weighed according to formula ratio by weighing instrument, it is stand-by;
2)Dissolving is mixed:By step 1)The yeast extract, molasses, inorganic salts, the monosodium glutamate desalination solution that weigh are dissolved in 30-50 DEG C of water respectively
Middle 20-40min, is extracted into Medium Proportion bucket by water pump, and self-loopa in bucket is carried out using main frame, and culture medium is obtained;
3)The treatment of ultra micro physics and chemistry:Ultra micro granulated processed is carried out while culture medium self-loopa;
4)PH is adjusted:By step 3)Culture medium after treatment is evacuated in fermentation tank, to addition caustic soda in fermentation tank to medium pH
It is 6-7;
5)Inoculation:To addition step 1 in fermentation tank)The microbial deodorant stoste of weighing, self-loopa is mixed;
6)Fermentation:Fermentation is initial to carry out interior circulation, and subsequent sealing and fermenting is obtained microbial deodorant, micro- life after the completion of fermentation
The living bacteria count that thing deodorant contains lactic acid bacteria is hundred million/ml of 7-15, and the living bacteria count of bacillus is hundred million/ml of 1-5,
The living bacteria count of acetic acid bacteria is hundred million/ml of 2-8, and the living bacteria count of saccharomycete is hundred million/ml of 0.5-2.
Described a kind of microbial deodorant, it is characterised in that the inorganic salts include following element:Na、K、Ca、Mg、
Cl、Fe、Mn、Zn、Cu。
Described a kind of microbial deodorant, it is characterised in that the monosodium glutamate desalination solution is remaining liquid after production glutamic acid
Body isolates the remaining mother liquor of ammonium sulfate again, mainly including glutamic acid, L-aminobutanedioic acid, glycine and alanine.
A kind of described microbial deodorant, it is characterised in that the lactic acid bacteria be Lactobacillus plantarum, Lactobacillus rhamnosus,
One or more in Lactobacillus pentosus and lactobacillus paracasei;The bacillus is bacillus subtilis, solution starch
One or more in bacillus and lysine bacillus;The acetic acid bacteria is acetobacter;The saccharomycete is
Saccharomyces cerevisiae.
Described a kind of microbial deodorant, it is characterised in that the microbial deodorant stoste is obtained by wormwood water extraction,
The wormwood extraction process by water uses conventional fabrication process.
A kind of described microbial deodorant, it is characterised in that step 2)In, yeast extract dissolving water consumption accounts for water inventory
1%, molasses dissolving water consumption accounts for the 25% of water inventory, and inorganic salts dissolving water consumption accounts for the 1% of water inventory, and monosodium glutamate desalination solution dissolving is used
Water accounts for the 8% of water inventory.
A kind of described microbial deodorant, it is characterised in that step 2)In, each raw material after dissolving is in Medium Proportion
Self-loopa 2-4h in bucket;Step 3)Middle culture medium ultra micro granulated processed 1-3h in Medium Proportion bucket.
A kind of described microbial deodorant, it is characterised in that each raw material self-loopa in Medium Proportion bucket after dissolving
2.5h;Culture medium ultra micro granulated processed 2h in Medium Proportion bucket.
A kind of described microbial deodorant, it is characterised in that step 4)The pH value of middle culture medium is adjusted to 6.7.
A kind of described microbial deodorant, it is characterised in that step 6)In, ferment initial interior circulation 48-72h, circulates 4-
8 times, subsequent sealing and fermenting 24-68h.
A kind of microbial deodorant and production technology that the present invention is provided, using existing installation for fermenting, saving puts into
This, shortens fermentation period, and easy to operate, quantities is small, and effect is obvious, non-secondary pollution, while also effectively by monosodium glutamate desalination
Liquid is recycled.And the deodorant stoste for using for Chinese medicine in extract, being used in deodorant microorganism fungus kind can assist
Same Fermentative growth, can be bred with syntrophism, it is to avoid the microorganism fungus kind that point tank culture is remixed occurs the phenomenon of antagonism,
So as to further ensure the stability and deodorizing effect of the microbial deodorant product that present invention process is produced.
Specific embodiment
The present invention is further illustrated below in conjunction with specification specific embodiment.
In order to more fully disclose a kind of microbial deodorant of the invention and production technology, said with reference to example
It is bright.It is below preferred embodiment of the invention, further describes the present invention, but the present invention is not limited only to this.
Embodiment 1
1)2Kg yeast extracts, 60Kg molasses, 3Kg inorganic salts, 30Kg monosodium glutamate desalination solutions and 905Kg water are accurately weighed first;2)Respectively
With 35 DEG C of 10Kg, 250Kg, 10Kg, 80Kg of water dissolves yeast extract, molasses, inorganic salts and monosodium glutamate desalination solution 30min, pass through
Water pump is extracted into Medium Proportion bucket, and self-loopa 2.5h in bucket is carried out using main frame;3)Carried out while culture medium self-loopa
Ultra micro granulated processed 2h;4)Will be by step 3)Culture medium after treatment is evacuated in fermentation tank, and caustic soda is added extremely in fermentation tank
Medium's PH Value is to 6.7;5)Inoculation, to the microbial deodorant stoste that 50Kg is added in fermentation tank, self-loopa is mixed;6)Hair
Ferment, the initial 72h that ferments carries out self-loopa 8 times, subsequent sealing and fermenting 144h.Wherein, microbial deodorant stoste is from Chinese medicine
Wormwood reclaimed water puts forward, and its extraction process by water is Conventional process steps, and monosodium glutamate desalination solution is remaining liquid after production glutamic acid
The remaining mother liquor of ammonium sulfate is isolated again, mainly including glutamic acid, L-aminobutanedioic acid, glycine and alanine.
Embodiment 2
1)4Kg yeast extracts, 100Kg molasses, 3Kg inorganic salts, 30Kg monosodium glutamate desalination solutions and 863Kg water are accurately weighed first;2)Point
Not Yong 35 DEG C of 10Kg, 250Kg, 10Kg, 80Kg of water dissolves yeast extract, molasses, inorganic salts and monosodium glutamate desalination solution 30min, lead to
Cross water pump to be extracted into Medium Proportion bucket, self-loopa 2.5h in bucket is carried out using main frame;3)Enter while culture medium self-loopa
Row ultra micro granulated processed 2h;4)By 3)Culture medium after treatment is evacuated in fermentation tank, to the caustic soda added in fermentation tank to culture
Base pH value is 6.7;5)Inoculation, to the microbial deodorant stoste that 50Kg is added in fermentation tank, self-loopa is mixed;6)Fermentation, hair
The initial 72h of ferment carries out self-loopa 8 times, subsequent sealing and fermenting 168h.Wherein, microbial deodorant stoste is from Chinese medicine wormwood
Out, its extraction process by water is Conventional process steps for water extraction, monosodium glutamate desalination solution for after production glutamic acid remaining liquid separate again
Go out the remaining mother liquor of ammonium sulfate, mainly including glutamic acid, L-aminobutanedioic acid, glycine and alanine.
Embodiment 3
1)5Kg yeast extracts, 150Kg molasses, 5Kg inorganic salts, 50Kg monosodium glutamate desalination solutions and 790Kg water are accurately weighed first;2)Point
Not Yong 35 DEG C of 15Kg, 350Kg, 15Kg, 100Kg of water dissolves yeast extract, molasses, inorganic salts and monosodium glutamate desalination solution 30min, lead to
Cross water pump to be extracted into Medium Proportion bucket, self-loopa 4h in bucket is carried out using main frame;3)Carried out while culture medium self-loopa
Ultra micro granulated processed 3h;4)By 3)Culture medium after treatment is evacuated in fermentation tank, to the caustic soda added in fermentation tank to culture medium
PH value is 6.7;5)Inoculation, to the microbial deodorant stoste that 50Kg is added in fermentation tank, self-loopa is mixed;6)Fermentation, fermentation
Initial 72h carries out self-loopa 8 times, subsequent sealing and fermenting 168h.Wherein, microbial deodorant stoste is from Chinese medicine wormwood reclaimed water
Put forward, its extraction process by water is Conventional process steps, monosodium glutamate desalination solution is that remaining liquid is isolated again after producing glutamic acid
The remaining mother liquor of ammonium sulfate, mainly including glutamic acid, L-aminobutanedioic acid, glycine and alanine.
Embodiment 4
1)2Kg yeast extracts, 50Kg molasses, 2Kg inorganic salts, 25Kg monosodium glutamate desalination solutions and 966Kg water are accurately weighed first;2)Respectively
With 35 DEG C of 10Kg, 200Kg, 8Kg, 80Kg of water dissolves yeast extract, molasses, inorganic salts and monosodium glutamate desalination solution 30min, by water
Pumping to Medium Proportion bucket in, carry out self-loopa 2h in bucket using main frame;3)Ultra micro is carried out while culture medium self-loopa
Granulated processed 1h;4)By 3)Culture medium after treatment is evacuated in fermentation tank, to the caustic soda added in fermentation tank to Medium's PH Value
It is 6.7;5)Inoculation, to the microbial deodorant stoste that 50Kg is added in fermentation tank, self-loopa is mixed;6)Fermentation, fermentation is initial
72h carries out self-loopa 8 times, subsequent sealing and fermenting 144h.Wherein, microbial deodorant stoste is to be proposed from Chinese medicine wormwood reclaimed water
Come, its extraction process by water is Conventional process steps, monosodium glutamate desalination solution is that remaining liquid isolates sulfuric acid again after producing glutamic acid
The remaining mother liquor of ammonium, mainly including glutamic acid, L-aminobutanedioic acid, glycine and alanine.
Experimental example 1:Microbial deodorant application test
In Jinhua house refuse terminal, by 50 times of running water of the microbial deodorant in embodiment 1(Volume ratio), use
Spray gun uniformly sprays 1L, and foul smell, ammonia and concentration of hydrogen sulfide are detected after effect 1.5h, and testing result is as shown in table 1:
The deodorizing effect of the microbial deodorant of the embodiment 1 of table 1
Project | Before sprinkling | After sprinkling | Reduced rate(%) |
104 | 10 | 90.38 | |
1.03 | 0.092 | 91.07 | |
0.087 | 0.007 | 91.95 |
As shown in Table 1, after the microbial deodorant 1.5h of house refuse terminal sprinkling embodiment 1, foul smell, ammonia and hydrogen sulfide
Substantially reduced before concentration ratio sprinkling, wherein odor concentration reduces 90.38%, and ammonia concentration reduces 91.07%, concentration of hydrogen sulfide
Reduce 91.95%.
Experimental example 2:Microbial deodorant application test
In Hangzhou refuse landfill, by 50 times of running water of the microbial deodorant in embodiment 2(Volume ratio), use spray gun
Uniform sprinkling 1L, foul smell, ammonia and concentration of hydrogen sulfide are detected after effect 1.5h, and testing result is as shown in table 2:
The deodorizing effect of the microbial deodorant of the embodiment 2 of table 2
Project | Before sprinkling | After sprinkling | Reduced rate(%) |
5630 | 112 | 98.01 | |
1.25 | 0.06 | 95.2 | |
18.75 | 1.57 | 91.63 |
As shown in Table 2, after the microbial deodorant 1.5h of refuse landfill sprinkling embodiment 2, foul smell, ammonia and concentration of hydrogen sulfide
Than substantially being reduced before sprinkling, wherein odor concentration reduces 98.01%, and ammonia concentration reduces 95.2%, concentration of hydrogen sulfide reduction
91.63%.
Contrast experiment 1:With other microbial deodorant products application contrast tests
In Hangzhou refuse landfill, by the microbial deodorant in embodiment 2 and other deodorant products respectively with originally
50 times of water(Volume ratio), 1L is uniformly sprayed with spray gun, detect foul smell, ammonia and concentration of hydrogen sulfide, testing result after effect 1.5h
As shown in table 3:
The contrast deodorizing effect of table 3 and other microbial deodorants
Project | Before sprinkling | After this product sprinkling | After competing product sprinkling | This product reduced rate(%) | Other product reduced rates(%) |
5630 | 112 | 115 | 98.01 | 97.96 | |
1.25 | 0.06 | 0.07 | 95.2 | 94.4 | |
18.75 | 1.57 | 2.51 | 91.63 | 86.61 |
As shown in Table 3, the microbial deodorant of refuse landfill sprinkling embodiment 2 and other companies microbial deodorants 1.5h
Afterwards, foul smell, ammonia and concentration of hydrogen sulfide before sprinkling than substantially reducing, but the microbial deodorant effect of embodiment 2 is better than
Other company's microbial deodorants, are mainly manifested in hydrogen sulfide removal rate, the gemma contained in the microbial deodorant of embodiment 2
Bacillus quantity is higher, is conducive to the removal of hydrogen sulfide.
Finally, in addition it is also necessary to it is noted that listed above is only several specific embodiments of the invention.Obviously, this hair
It is bright to be not limited to above example, there can also be many deformations.One of ordinary skill in the art can be from present disclosure
The all deformations directly derived or associate, are considered as protection scope of the present invention.
Claims (10)
1. a kind of microbial deodorant, it is characterised in that formed by the material combination of following weight proportion:The yeast of 0.2-0.5%
Cream, the molasses of 5-15%, the inorganic salts of 0.2-0.5%, the monosodium glutamate desalination solution of 2.5-5%, the microbial deodorant stoste of 5-10%,
The water of 70-80%, the microbial deodorant is prepared from using following processing step:
1)Weigh:Each raw material is weighed according to formula ratio by weighing instrument, it is stand-by;
2)Dissolving is mixed:By step 1)The yeast extract, molasses, inorganic salts, the monosodium glutamate desalination solution that weigh are dissolved in 30-50 DEG C of water respectively
Middle 20-40min, is extracted into Medium Proportion bucket by water pump, and self-loopa in bucket is carried out using main frame, and culture medium is obtained;
3)The treatment of ultra micro physics and chemistry:Ultra micro granulated processed is carried out while culture medium self-loopa;
4)PH is adjusted:By step 3)Culture medium after treatment is evacuated in fermentation tank, to addition caustic soda in fermentation tank to medium pH
It is 6-7;
5)Inoculation:To addition step 1 in fermentation tank)The microbial deodorant stoste of weighing, self-loopa is mixed;
6)Fermentation:Fermentation is initial to carry out interior circulation, and subsequent sealing and fermenting is obtained microbial deodorant, micro- life after the completion of fermentation
The living bacteria count that thing deodorant contains lactic acid bacteria is hundred million/ml of 7-15, and the living bacteria count of bacillus is hundred million/ml of 1-5,
The living bacteria count of acetic acid bacteria is hundred million/ml of 2-8, and the living bacteria count of saccharomycete is hundred million/ml of 0.5-2.
2. a kind of microbial deodorant as claimed in claim 1, it is characterised in that the inorganic salts include following element:Na、
K、Ca、Mg、Cl、Fe、Mn、Zn、Cu。
3. a kind of microbial deodorant as claimed in claim 1, it is characterised in that the monosodium glutamate desalination solution is production glutamic acid
Remaining liquid isolates the remaining mother liquor of ammonium sulfate again afterwards, mainly including glutamic acid, L-aminobutanedioic acid, glycine and alanine.
4. a kind of microbial deodorant as claimed in claim 1, it is characterised in that the lactic acid bacteria is Lactobacillus plantarum, sandlwood
One or more in sugared lactobacillus, Lactobacillus pentosus and lactobacillus paracasei;The bacillus is bacillus subtilis
One or more in bacterium, bacillus amyloliquefaciens and lysine bacillus;The acetic acid bacteria is acetobacter;Institute
Saccharomycete is stated for saccharomyces cerevisiae.
5. a kind of microbial deodorant as claimed in claim 1, it is characterised in that the microbial deodorant stoste is by wormwood
Water extraction is obtained.
6. a kind of microbial deodorant as claimed in claim 1, it is characterised in that step 2)In, yeast extract dissolving water consumption is accounted for
The 1% of water inventory, molasses dissolving water consumption accounts for the 25% of water inventory, and inorganic salts dissolving water consumption accounts for the 1% of water inventory, monosodium glutamate desalination
Liquid dissolving water consumption accounts for the 8% of water inventory.
7. a kind of microbial deodorant as claimed in claim 1, it is characterised in that step 2)In, each raw material after dissolving is in training
Basigamy is supported than self-loopa 2-4h in bucket;Step 3)Middle culture medium ultra micro granulated processed 1-3h in Medium Proportion bucket.
8. a kind of microbial deodorant as claimed in claim 7, it is characterised in that each raw material after dissolving is in Medium Proportion
Self-loopa 2.5h in bucket;Culture medium ultra micro granulated processed 2h in Medium Proportion bucket.
9. a kind of microbial deodorant as claimed in claim 1, it is characterised in that step 4)The pH value of middle culture medium adjust to
6.7。
10. a kind of microbial deodorant as claimed in claim 1, it is characterised in that step 6)In, ferment initial interior circulation 48-
72h, circulates 4-8 times, subsequent sealing and fermenting 24-168h.
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Cited By (7)
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CN107164284A (en) * | 2017-07-10 | 2017-09-15 | 佛山市水创科联生态科技有限公司 | A kind of deodorizing microorganism microbial inoculum and preparation method thereof |
CN108097032A (en) * | 2017-12-29 | 2018-06-01 | 广州广牧丰生物技术有限公司 | A kind of farm's deodorant and the method that poultry production performance is improved with the deodorant |
CN110343649A (en) * | 2019-08-23 | 2019-10-18 | 浙江中益生物环保科技有限公司 | A kind of microbial deoderizer and its application |
CN110438031A (en) * | 2019-06-28 | 2019-11-12 | 中南大学 | A kind of microbial deoderizer and preparation method thereof |
CN110482711A (en) * | 2019-07-03 | 2019-11-22 | 东莞市顶盛环保科技有限公司 | A kind of bacteria agent and preparation method thereof for deodorization |
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