CN109603514A - A kind of microbial deodorant and preparation method thereof - Google Patents
A kind of microbial deodorant and preparation method thereof Download PDFInfo
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
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- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/72—Organic compounds not provided for in groups B01D53/48 - B01D53/70, e.g. hydrocarbons
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01D2251/95—Specific microorganisms
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/70—Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
- B01D2257/702—Hydrocarbons
- B01D2257/7027—Aromatic hydrocarbons
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/90—Odorous compounds not provided for in groups B01D2257/00 - B01D2257/708
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- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The present invention provides a kind of microbial deodorant and preparation method thereof, and the microbial deodorant contains denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum, cloth Laplace saccharomycete and photosynthetic bacteria.Microbial deodorant provided by the invention has stronger adaptability to environment, can be proliferated growth rapidly, and stabilization occupies ecological niche, effectively inhibits the spoilage organisms for generating odorant, achievees the purpose that fast deodorization；Pass through the different role of microorganism in deodorant in the environment; collaborative work can not only effectively remove rubbish corruption bring foul odour; for a long time using the decomposition that can also accelerate rubbish organic matter; reduce the osmosis pollution to soil near landfill yard; be conducive to the subsequent protection afforestation ecological of landfill yard; and it is had broad application prospects by the synergistic effect between microorganism so that the lasting deodorizing effect of deodorant provided by the invention is also more preferable.
Description
Technical field
The present invention relates to deodorant technical fields more particularly to a kind of microbial deodorant and preparation method thereof.
Background technique
China's domestic waste year output is about 1.4 hundred million tons, in addition to except small part burning, compost or recycling,
80% or more, which is transported to refuse landfill, carries out landfill disposal, and the whole nation shares about 400 refuse landfills according to incompletely statistics.
Rubbish can generate the foul gas of a large amount of strong stimulations during transhipment, tiling, compacting, processing and landfill, to refuse landfill
And the environment on periphery causes greatly to pollute, and influences the health of sanitationman and nearby residents.So rubbish is effectively treated
Landfill yard rubbish generate odor pollution, reduce its broken ring to periphery ecological environment, protect the health of the people, at
One important content of garbage management.
The existing garbage deodorant of existing market mainly has chemical deodorizing agent, physical deodorization agent and microbial deodorant.Change
The use for learning deodorant can not completely remove odoriferous thing, and deodorizing effect is insufficient to be not thorough, and lasting effect is poor.Physical method is logical
It crosses and stink substances is absorbed into the micropore of absorbent using absorbent, but absorbent absorbs stink substances and is difficult to desorb, it can not
It reuses, causes the secondary pollution of environment.Microbial deodorant technology is the physiological metabolism activity degradation stench using microorganism
Substance achievees the purpose that deodorization, is free of any chemicals, will not cause secondary pollution, and can reach and continue deodorization for a long time,
Low production cost.But the microorganism in existing microbial deodorant tends not to adapt to landfill yard rugged environment, sprinkling
Primary growth breeding is slower afterwards, it is difficult to achieve the purpose that fast deodorization, there is also microorganism compossibility is poor, degradation hydrogen sulfide and
The ineffective problem of ammonia limits the further development of microbial deodorant.
Summary of the invention
Poor to environmental suitability for existing microbial deodorant, compossibility is poor between microorganism leads to deodorizing effect
Bad problem, the present invention provide a kind of microbial deodorant and preparation method thereof.
In order to solve the above technical problems, present invention provide the technical scheme that
A kind of microbial deodorant, the microbial deodorant contain denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum,
Cloth Laplace saccharomycete and photosynthetic bacteria.
Compared with the existing technology, the present invention constructs efficient deodorization bacterial strain group according to the complexity of refuse odor ingredient
Close, the compound strain of selection can long-term co-existence, can be rooted in rubbish rapidly after sprinkling, form stable microecosystem, into
Row quickly breeding, forms dominant microflora, reaches quick, permanent sustainable deodorizing effect；Denitrogenation micrococcus and photosynthetic bacteria, can
A variety of nutriments such as glucide, nitrogen compound, amino acid are synthesized, are provided a favorable guarantee for the breeding of other bacteriums, together
When also using the metabolite of other microorganisms as itself nutriment, ensure that the growth and breeding of itself.The present invention mentions
The microbial deodorant of confession, various bacteria synergistic effect, generates a variety of enzyme materials and metabolite, so that deodorant is adsorbing
While foul gas is that itself is utilized in air, thallus and its metabolite can also inhibit the work of putrefactive microorganisms in rubbish
It is dynamic, meanwhile, a variety of enzyme materials of secretion can also decompose the organic substance for producing foul smell, the source generated by reducing foul gas
And the foul gas generated is eliminated, fundamentally reach thorough deodorization purpose.
Denitrogenation micrococcus is nitrification heterotrophicy bacteria, and not only growth rate is fast, acidic environment is resistant to, in anoxia condition
Under, using denitrification or denitrification, it is nitrogen by nitrate or nitrate reductase, reduces the discharge amount of ammonia,
Effectively reduce the generation of stench.
Clostridium butyricum is gram-positive anaerobic bacterium, is added in feed generally as feed addictive.The present invention is unexpected
It was found that clostridium butyricum is cooperateed with work there are good with denitrogenation micrococcus, lactobacillus plantarum, cloth Laplace saccharomycete and photosynthetic bacteria
With being remarkably improved the deodorizing effect of compound bacteria.Clostridium butyricum has heat-resisting acidproof ability, can decompose various carbon hydrates
Object, sugar, starch, the final product for hydrolyzing starch and carbohydrate is butyric acid, acetic acid and lactic acid and a small amount of propionic acid and formic acid, shape
At the acidic environment for being unfavorable for putrefactive microorganisms life；Its a variety of enzyme secreted, such as protease, cellulase, glycosidase, shallow lake
Powder enzyme, pectase etc. can effectively decompose organic substance, organic sulfur compound, the organic nitrogen etc. for producing stink, and fundamentally degradation generates
The substance of foul gas.
Lactobacillus plantarum is one kind of lactic acid bacteria, is anaerobism or facultative anaerobic bacteria, and 85% or more end product of metabolism is cream
Acid, forms acidic environment, and acid medium is conducive to NH3Absorption, while the metabolic activity of stench producing strains also can be effectively suppressed,
Reduce the generation of foul smell.Lactobacillus plantarum, clostridium butyricum and denitrogenation micrococcus are synergistic in the present invention, can effectively reduce NH3
Yield and effectively absorb the NH generated3, hence it is evident that it reduces by NH3Caused foul odour.
Cloth Laplace Blastocystis is heat-resist in facultative anaerobic bacteria, is widely used in preventing and treating various diarrhea and intestines
Road inflammation, cloth Laplace saccharomycete can enhance other several microorganisms to landfill yard adverse circumstances in deodorant provided by the invention
Adaptability enhances microecological balance, improves the deodorizing effect of deodorant.
Photosynthetic bacteria is a kind of autotrophy microorganism, it can be using the solar thermal energy of soil receiving or using ultraviolet light as energy
The hydrogen in hydrogen sulfide and hydrocarbon is separated in source, achievees the purpose that remove the odorants such as hydrogen sulfide and methane；Together
When, photosynthetic bacteria can also synthesize carbohydrate, amino with organic matter, pernicious gas (hydrogen sulfide etc.) and carbon dioxide, nitrogen etc. for matrix
Acid, vitamins, nitrogen compound and physiological activator etc. provide nutrient for other microbial reproductions.Light in the present invention
Bacterium and denitrogenation micrococcus synergistic effect are closed, is that the growth and breeding of other microorganisms improves nutrient, ensure that other microorganisms
Normal growth is quickly bred so that microorganism can rapidly adapt to the adverse circumstances of landfill yard, and competition forms strain advantage.
Preferably, the concentration of the denitrogenation micrococcus is 1.5 × 108~2.0 × 108CFU/ml, the clostridium butyricum it is dense
Degree is 5 × 107~8 × 107CFU/ml, the concentration of the lactobacillus plantarum are 7 × 107~10 × 107CFU/ml, the Bradley
The concentration of family name's saccharomycete is 3 × 107~5 × 107CFU/ml, the concentration of the photosynthetic bacteria are 1.0 × 108~1.5 ×
108CFU/ml。
The concentration of preferred compound strain, after each bacterial strain can be made to be sprayed onto rubbish using the organic matter in rubbish as
Nutriment carries out quick growth and breeding, also helps long-term co-existence between each bacterial strain, will not producing bacterial strain growth vigor, from
And achieve the purpose that the permanent deodorization of quick and sustainable progress.
Preferably, further include the raw material of following quality percent by volume: sodium benzoate 0.05~0.1% and glycerol 1~
2%.
Sodium benzoate and glycerol can play the role of protecting microorganism, extend the storage life of deodorant.
The present invention also provides a kind of preparation methods of microbial deodorant, include the following steps:
Step a, aseptically, by denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum, cloth Laplace saccharomycete and light
It closes bacterium to be seeded in seed culture medium expands culture respectively, culture is to bacterial strain concentration up to 2 × 108CFU/ml or more,
Respectively obtain the seed liquor of each bacterial strain；
The seed liquor of each bacterial strain is seeded in fermentation medium respectively and carries out fermented and cultured by step b, culture to bacterium
Strain concentration reaches 2 × 108CFU/ml or more respectively obtains the fermentation liquid of each bacterial strain；
Step c takes fermentation liquid, sodium benzoate, glycerol and the purified water of each bacterial strain, is prepared by design,
Obtain microbial deodorant.
Deodoriser preparing process of the invention is simple, and process flow is short, low in cost, easy to use, under room temperature save be
Can, and validity period is up to six months or more.
Preferably, the culture pH of the denitrogenation micrococcus is 6.5~7.2, and cultivation temperature is 30~32 DEG C, and incubation time is
24~28h.
Preferably, the clostridium butyricum culture pH be 7.0~7.2, cultivation temperature be 29~31 DEG C, incubation time be 48~
72h。
Preferably, the culture pH of the lactobacillus plantarum is 7.0~7.2；Cultivation temperature is 35~37 DEG C, and incubation time is
24~28h.
Preferably, the culture pH of the cloth Laplace saccharomycete is 5.5~6.5；Cultivation temperature is 28~30 DEG C, incubation time
For 32~36h.
Preferably, the culture pH of the photosynthetic bacteria is 7.0~7.2；Cultivation temperature is 30~32 DEG C, incubation time 30
~35h.
Preferred culture condition of culture is conducive to the growth and breeding of bacterium, obtains the culture bacterium of high activity.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
A kind of microbial deodorant, the microbial deodorant contain denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum,
Cloth Laplace saccharomycete and photosynthetic bacteria, wherein the concentration of denitrogenation micrococcus is 1.5 × 10 in microbial deodorant8CFU/ml, fourth
The concentration of sour clostridium is 7 × 107CFU/ml, the concentration of lactobacillus plantarum are 10 × 107CFU/ml, the concentration of cloth Laplace saccharomycete
It is 3 × 107CFU/ml, the concentration of photosynthetic bacteria are 1.5 × 108CFU/ml。
The preparation method of mentioned microorganism microbial inoculum includes the following steps:
Step a, aseptically, by denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum, cloth Laplace saccharomycete and light
It closes bacterium to be seeded in seed culture medium expands culture respectively, culture is to bacterial strain concentration up to 2 × 108CFU/ml or more,
Respectively obtain the seed liquor of each bacterial strain；
Step b takes the seed liquor of each bacterial strain 10% (V/V) to be seeded in fermentation medium respectively and carries out fermented and cultured, training
It supports to bacterial strain concentration and reaches 2 × 108CFU/ml or more respectively obtains the fermentation liquid of each bacterial strain；
Step c carries out bacterium number counting to the fermentation liquid of each bacterial strain, according to the additional amount of each fermentation liquid of above-mentioned concentration calculation,
It takes each fermentation liquid to be added in purified water respectively, 0.05% sodium benzoate and 1% glycerol is added, be uniformly mixed, obtain microorganism
Deodorant.
The seed culture fluid of the denitrogenation micrococcus and the formula of fermentation liquid are as follows: glucose 3%, peptone 1%, yeast leaching
Powder 0.5%, sodium chloride 0.3%, distilled water 95.2%, pH 6.8；Cultivation temperature is 31 DEG C, incubation time 26h.
The seed culture fluid of the clostridium butyricum and the formula of fermentation liquid are as follows: yeast powder 0.5%, peptone 1%, sodium chloride
1%, distilled water 97.5%, pH7.1；Cultivation temperature is 30 DEG C, incubation time 60h.
The seed culture fluid of the lactobacillus plantarum and the formula of fermentation liquid are as follows: corn pulp 4%, sucrose 2%, sodium acetate
0.3%, citric acid hydrogen diamine 0.7%, magnesium sulfate 0.05%, Tween 80 0.5%, distilled water 92.45%, pH7.1；Culture temperature
Degree is 36 DEG C, incubation time 26h.
The seed culture fluid of the cloth Laplace saccharomycete and the formula of fermentation liquid are as follows: yeast powder 1%, peptone 2%, grape
Sugar 2%, distilled water 95%, pH6.0；Cultivation temperature is 29 DEG C, incubation time 34h.
The seed culture fluid of the photosynthetic bacteria and the formula of fermentation liquid are as follows: glucose 3%, peptone 1%, yeast powder
2.5%, sodium chloride 0.3%, distilled water 93.2%, pH7.1；Cultivation temperature is 31 DEG C, incubation time 32h.
Embodiment 2
A kind of microbial deodorant, the microbial deodorant contain denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum,
Cloth Laplace saccharomycete and photosynthetic bacteria, wherein the concentration of denitrogenation micrococcus is 1.8 × 10 in microbial deodorant8CFU/ml, fourth
The concentration of sour clostridium is 5 × 107CFU/ml, the concentration of lactobacillus plantarum are 8 × 107The concentration of CFU/ml, cloth Laplace saccharomycete is
5×107CFU/ml, the concentration of photosynthetic bacteria are 1.0 × 108CFU/ml。
The preparation method of mentioned microorganism microbial inoculum includes the following steps:
Step a, aseptically, by denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum, cloth Laplace saccharomycete and light
It closes bacterium to be seeded in seed culture medium expands culture respectively, culture is to bacterial strain concentration up to 2 × 108CFU/ml or more,
Respectively obtain the seed liquor of each bacterial strain；
Step b takes the seed liquor of each bacterial strain 10% (V/V) to be seeded in fermentation medium respectively and carries out fermented and cultured, training
It supports to bacterial strain concentration and reaches 2 × 108CFU/ml or more respectively obtains the fermentation liquid of each bacterial strain；
Step c carries out bacterium number counting to the fermentation liquid of each bacterial strain, according to the additional amount of each fermentation liquid of above-mentioned concentration calculation,
It takes each fermentation liquid to be added in purified water respectively, 0.05% sodium benzoate and 1% glycerol is added, be uniformly mixed, obtain microorganism
Deodorant.
The seed culture fluid of the denitrogenation micrococcus and the formula of fermentation liquid are as follows: glucose 3%, peptone 1%, yeast leaching
Powder 0.5%, sodium chloride 0.3%, distilled water 95.2%, pH 6.5；Cultivation temperature is 30 DEG C, incubation time 28h.
The seed culture fluid of the clostridium butyricum and the formula of fermentation liquid are as follows: yeast powder 0.5%, peptone 1%, sodium chloride
1%, distilled water 97.5%, pH7.0；Cultivation temperature is 29 DEG C, incubation time 72h.
The seed culture fluid of the lactobacillus plantarum and the formula of fermentation liquid are as follows: corn pulp 4%, sucrose 2%, sodium acetate
0.3%, citric acid hydrogen diamine 0.7%, magnesium sulfate 0.05%, Tween 80 0.5%, distilled water 92.45%, pH7.0；Culture temperature
Degree is 35 DEG C, incubation time 28h.
The seed culture fluid of the cloth Laplace saccharomycete and the formula of fermentation liquid are as follows: yeast powder 1%, peptone 2%, grape
Sugar 2%, distilled water 95%, pH5.5；Cultivation temperature is 28 DEG C, incubation time 36h.
The seed culture fluid of the photosynthetic bacteria and the formula of fermentation liquid are as follows: glucose 3%, peptone 1%, yeast powder
2.5%, sodium chloride 0.3%, distilled water 93.2%, pH7.2；Cultivation temperature is 30 DEG C, incubation time 35h.
Embodiment 3
A kind of microbial deodorant, the microbial deodorant contain denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum,
Cloth Laplace saccharomycete and photosynthetic bacteria, wherein the concentration of denitrogenation micrococcus is 2.0 × 10 in microbial deodorant8CFU/ml, fourth
The concentration of sour clostridium is 8 × 107CFU/ml, the concentration of lactobacillus plantarum are 7 × 107The concentration of CFU/ml, cloth Laplace saccharomycete is
4×107CFU/ml, the concentration of photosynthetic bacteria are 1.2 × 108CFU/ml。
The preparation method of mentioned microorganism microbial inoculum includes the following steps:
Step a, aseptically, by denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum, cloth Laplace saccharomycete and light
It closes bacterium to be seeded in seed culture medium expands culture respectively, culture is to bacterial strain concentration up to 2 × 108CFU/ml or more,
Respectively obtain the seed liquor of each bacterial strain；
Step b takes the seed liquor of each bacterial strain 10% (V/V) to be seeded in fermentation medium respectively and carries out fermented and cultured, training
It supports to bacterial strain concentration and reaches 2 × 108CFU/ml or more respectively obtains the fermentation liquid of each bacterial strain；
Step c carries out bacterium number counting to the fermentation liquid of each bacterial strain, according to the additional amount of each fermentation liquid of above-mentioned concentration calculation,
It takes each fermentation liquid to be added in purified water respectively, 0.05% sodium benzoate and 1% glycerol is added, be uniformly mixed, obtain microorganism
Deodorant.
The seed culture fluid of the denitrogenation micrococcus and the formula of fermentation liquid are as follows: glucose 3%, peptone 1%, yeast leaching
Powder 0.5%, sodium chloride 0.3%, distilled water 95.2%, pH 7.2；Cultivation temperature is 32 DEG C, and incubation time is for 24 hours.
The seed culture fluid of the clostridium butyricum and the formula of fermentation liquid are as follows: yeast powder 0.5%, peptone 1%, sodium chloride
1%, distilled water 97.5%, pH7.2；Cultivation temperature is 31 DEG C, incubation time 48h.
The seed culture fluid of the lactobacillus plantarum and the formula of fermentation liquid are as follows: corn pulp 4%, sucrose 2%, sodium acetate
0.3%, citric acid hydrogen diamine 0.7%, magnesium sulfate 0.05%, Tween 80 0.5%, distilled water 92.45%, pH7.2；Culture temperature
Degree is 37 DEG C, and incubation time is for 24 hours.
The seed culture fluid of the cloth Laplace saccharomycete and the formula of fermentation liquid are as follows: yeast powder 1%, peptone 2%, grape
Sugar 2%, distilled water 95%, pH6.5；Cultivation temperature is 30 DEG C, incubation time 32h.
The seed culture fluid of the photosynthetic bacteria and the formula of fermentation liquid are as follows: glucose 3%, peptone 1%, yeast powder
2.5%, sodium chloride 0.3%, distilled water 93.2%, pH7.2；Cultivation temperature is 32 DEG C, incubation time 30h.
Comparative example 1
This comparative example provides a kind of microbial deodorant, the microbial deodorant contain denitrogenation micrococcus, clostridium butyricum,
Lactobacillus plantarum, candidiasis and photosynthetic bacteria, wherein in microbial deodorant denitrogenation micrococcus concentration be 2.0 ×
108CFU/ml, the concentration of clostridium butyricum are 8 × 107CFU/ml, the concentration of lactobacillus plantarum are 7 × 107CFU/ml, Candida
The concentration of bacterium is 4 × 107CFU/ml, the concentration of photosynthetic bacteria are 1.2 × 108CFU/ml。
The seed culture fluid and fermentation liquid of candidiasis are identical as the cloth Laplace saccharomycete in embodiment 1, above-mentioned micro- life
The preparation method of object deodorant is same as Example 1, and details are not described herein again.
Comparative example 2
This comparative example provides a kind of microbial deodorant, and the microbial deodorant contains denitrogenation micrococcus, actinomyces, plant
Object lactobacillus, cloth Laplace saccharomycete and photosynthetic bacteria, wherein in microbial deodorant denitrogenation micrococcus concentration be 2.0 ×
108CFU/ml, the concentration of actinomyces are 8 × 107CFU/ml, the concentration of lactobacillus plantarum are 7 × 107CFU/ml, candidiasis
Concentration be 4 × 107CFU/ml, the concentration of photosynthetic bacteria are 1.2 × 108CFU/ml。
The seed liquid culture medium and fermentation liquid of actinomyces are starch nitrate culture-medium (Gause I culture medium), culture temperature
Degree is 30 DEG C, incubation time 60h.
The preparation method of mentioned microorganism deodorant is same as Example 1, and details are not described herein again.
Deodorizing effect test
600 square meter household refuse landfill sites of Shenyang are selected, garbage loading embeading amount is 1200 tons, sprays deodorization in 5-8 month
Agent applies embodiment 1 and comparative example 1, deodorant prepared by comparative example 2 respectively, and dosage is 6L, and when application uses purified water respectively
50 times of dilution is configured to dilution bacterium solution, with atomizers spray, tests after 2h, test result is as shown in table 1.
Table 1
As can be seen from the above table, after the microbial deodorant 2h that prepared by application embodiment 1, the degradation rate of ammonia reaches
93.7%, the degradation rate of hydrogen sulfide reaches 90.8%, and the degradation rate of styrene has reached 92.7%, and odor concentration is before using
549 are down to 19, and foul smell degrades 96.5%.
After applying microbial deodorant 2h prepared by comparative example 1, the degradation rate of ammonia reaches 80.6%, the degradation of hydrogen sulfide
Rate has reached 73.8%, and the degradation rate of styrene has reached 88.6%, and odor concentration 553 is down to 76 before using, foul smell drop
Solve 86.3%.
After applying microbial deodorant 2h prepared by comparative example 2, the degradation rate of hydrogen sulfide reaches 81.9%, the degradation of ammonia
Rate has reached 79.8%, and the degradation rate of styrene has reached 89.9%, and odor concentration 531 is down to 69 before using, foul smell drop
Solve 87%.
It is furthermore observed that the complex microbial inoculum of embodiment 1 also has preferable odor-resistant result after application 10 days,
And the complex microbial inoculum of comparative example 1 and comparative example 2 deodorization significant effect after using 5-7 days declines.
The above results explanation, the synergistic effect between microorganism after bacterial strain optimum organization of the present invention, syntrophism synbiosis
It changes opportunistic pathogen group and forms dominant microflora, preferably inhibit the generation of odorant, mentioned microorganism collective effect just has
The ability for inhibiting complex effluvium gas after lacking one of or several microorganisms, or selects other microorganisms to replace certain
After a kind of microorganism, the deodorizing capability of entire flora can declined, or even lose deodorization functions.
In conclusion microbial deodorant provided by the invention has stronger adaptability to environment, can increase rapidly
Reproductive growth, stabilization occupy ecological niche, effectively inhibit the spoilage organisms for generating odorant, achieve the purpose that fast deodorization；By removing
The different role of microorganism in the environment in smelly dose, collaborative work can not only effectively remove rubbish corruption bring foul odour,
For a long time using the decomposition that can also accelerate rubbish organic matter, the osmosis pollution to soil near landfill yard is reduced, is conducive to fill out
The subsequent protection afforestation ecological in field is buried, and by the synergistic effect between microorganism so that deodorant provided by the invention is held
Continuous deodorizing effect is also more preferable, has broad application prospects.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modification, equivalent replacement or improvement etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (9)
1. a kind of microbial deodorant, which is characterized in that the microbial deodorant contains denitrogenation micrococcus, clostridium butyricum, plant
Object lactobacillus, cloth Laplace saccharomycete and photosynthetic bacteria.
2. microbial deodorant as described in claim 1, which is characterized in that the concentration of the denitrogenation micrococcus is 1.5 × 108
~2.0 × 108CFU/ml, the concentration of the clostridium butyricum are 5 × 107~8 × 107CFU/ml, the concentration of the lactobacillus plantarum
It is 7 × 107~10 × 107CFU/ml, the concentration of the cloth Laplace saccharomycete are 3 × 107~5 × 107CFU/ml, it is described photosynthetic
The concentration of bacterium is 1.0 × 108~1.5 × 108CFU/ml。
3. microbial deodorant as claimed in claim 2, which is characterized in that further include the original of following quality percent by volume
Material: sodium benzoate 0.05~0.1% and glycerol 1~2%.
4. a kind of preparation method of microbial deodorant, which comprises the steps of:
Step a, aseptically, by denitrogenation micrococcus, clostridium butyricum, lactobacillus plantarum, cloth Laplace saccharomycete and photosynthetic thin
Bacterium is seeded in seed culture medium expands culture respectively, and culture is to bacterial strain concentration up to 2 × 108CFU/ml or more, respectively
Obtain the seed liquor of each bacterial strain；
The seed liquor of each bacterial strain is seeded in fermentation medium respectively and carries out fermented and cultured by step b, cultivates dense to bacterial strain
Degree reaches 2 × 108CFU/ml or more respectively obtains the fermentation liquid of each bacterial strain；
Step c takes fermentation liquid, sodium benzoate, glycerol and the purified water of each bacterial strain, matches according to the design of claim 3
It is prepared, obtains microbial deodorant.
5. the preparation method of microbial deodorant as claimed in claim 4, which is characterized in that the culture of the denitrogenation micrococcus
PH is 6.5~7.2, and cultivation temperature is 30~32 DEG C, and incubation time is 24~28h.
6. the preparation method of microbial deodorant as claimed in claim 4, which is characterized in that the clostridium butyricum culture pH is
7.0~7.2, cultivation temperature is 29~31 DEG C, and incubation time is 48~72h.
7. the preparation method of microbial deodorant as claimed in claim 4, which is characterized in that the culture of the lactobacillus plantarum
PH is 7.0~7.2；Cultivation temperature is 35~37 DEG C, and incubation time is 24~28h.
8. the preparation method of microbial deodorant as claimed in claim 4, which is characterized in that the training of the cloth Laplace saccharomycete
Supporting pH is 5.5~6.5；Cultivation temperature is 28~30 DEG C, and incubation time is 32~36h.
9. the preparation method of microbial deodorant as claimed in claim 4, which is characterized in that the culture pH of the photosynthetic bacteria
It is 7.0~7.2；Cultivation temperature is 30~32 DEG C, and incubation time is 30~35h.
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| CN111019863A (en) * | 2019-12-26 | 2020-04-17 | 青岛尚德生物技术有限公司 | Deodorizing microbial agent for removing odor in dry latrine and application thereof |
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| CN114250170A (en) * | 2021-12-01 | 2022-03-29 | 四川急客方便科技有限公司 | Fruit mixed fermentation deodorant and preparation method thereof |
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Application publication date: 20190412 |