CN102813948A - Preparation method of composite biological deodorant - Google Patents
Preparation method of composite biological deodorant Download PDFInfo
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- CN102813948A CN102813948A CN2011101501140A CN201110150114A CN102813948A CN 102813948 A CN102813948 A CN 102813948A CN 2011101501140 A CN2011101501140 A CN 2011101501140A CN 201110150114 A CN201110150114 A CN 201110150114A CN 102813948 A CN102813948 A CN 102813948A
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Abstract
The invention discloses a preparation method of a composite biological deodorant. The method is characterized in culturing a composite microbe flora. The composite microbe flora mainly comprises beneficial floras of bacillus, photosynthetic bacteria, lactic acid bacteria, bifidobacteria, yeasts, actinomycetes, and the like. Various metabolites produced during the growth of the flora assist in effectively removing foul odor in environment, and assist in improving the environment. Therefore, manure can be turned harmless and can be turned into a resource. The preparation method of the composite biological deodorant is that a facultative anaerobic culture method is combined with a special process, and a composite biological product is produced. The method is advantaged in simple fermentation process, no requirement on complicated equipment, high production efficiency, and low production cost. The method has good economic and social benefits. The method is suitable to be popularized in manure treatment plants, garbage stations, farms, and industrial and agricultural production and sewage discharging sites.
Description
Technical field
The present invention is specifically related to a kind of method for preparing of compound bio deodorizer, belongs to technical field of biological fermentation, is to utilize the multiple beneficial microorganism, and through special fermentation technology, preparation can suppress the noxious bacteria breeding, alleviates or eradicate the biological product of stink.
Background technology:
Feces treatment plant, refuse depot, plant and industrial and agricultural production blowdown cause foul smell to overflow, and severe contamination air and environment are sought effective, green deodorising product and method, become the urgent needs of present society.Both at home and abroad biological deodorant is often single or biologos not enough or cost is too high because of flora, has influenced greatly to use and promote.Know with regard to the inventor, utilizing the special fermentation technique of multiple beneficial microorganism, obtain the natural biological metabolite and be used for deodorization, improved efficient, reduced the technology of use cost, also do not have relevant report at present.
Summary of the invention:
The object of the invention provides a kind of and can effectively eliminate the foul odour in the environment exactly for overcoming above deficiency, improves environment, makes that muck is innoxious, resource becomes possibility, and.Fermentation technology is simple, does not need complex device, and production efficiency is high, and production cost is low, and the method for preparing of a kind of compound bio deodorizer of favorable economic benefit and social benefit is arranged.
Main technical schemes of the present invention is achieved in that multiple microorganism prepares the method for compound bio deodorizer, comprises the preparation of function strain, it is characterized in that taking purebred cultivation according to the physiological characteristics of strain, carries out compound then according to a certain percentage.Composite flora obtains the living bacterial cells and the metabolite of maximum quantity through the amphimicrobian fermentation mode.Described strain comprises the microorganism of bacillus, streptomyces, Saccharomycodes, genus lactubacillus, acetic acid Pseudomonas etc.Have various active enzyme and secondary metabolite, like materials such as protease, amylase, lipase, multiple organic acid, small-molecular peptides, vitamin, oligosaccharides.
Described amphimicrobian training method is to adopt deep liquid to leave standstill cultivation, selects appropriate media according to the needs of each strain, mainly is under anaerobic to make its fermentation, obtains the tunning of maximum.
The culture medium that described strain preparation is used has following component and preferable weight proportion:
Sucrose 10～30g peptone 4～10g yeast extract 5-10g KH
2PO
45～10g MgSO
41～6g water 1L
The culture medium that described strain deep liquid standing for fermentation is used has following component and preferable weight proportion:
Semen sojae atricolor hydrolysate 10～30g peptone 4～10g sucrose 16～24g KH
2PO
43～8g MgSO
41～6g water 1L.Be described further production technology below in conjunction with instance:
(1) spawn culture comprises the steps:
Slant strains: the test tube slant is in 25-32 ℃ of constant temperature culture, 24-72h.
Bacteria suspension: the test tube slant is poured sterilized water into, scrapes off lawn, the preparation suspension.
Fluid medium: the culture medium that the strain liquid culture is used has following component and preferable weight proportion:
Semen sojae atricolor hydrolysate 10～30g peptone 4～10g sucrose 16～24g KH
2PO
43～8g MgSO
41～6g water 1L.
Sterilization: 0.12-0.15Mpa, 121-125 ℃, steam sterilization 30 minutes.
Inoculation: bacteria suspension is inserted in the culture medium of sterilization.
Cultivate: 25-32 ℃, cultivate 36-72h.
(2) the strain deep liquid leaves standstill cultivation:
Liquid fermentation medium: sucrose 10～30g peptone 4～10g yeast extract 5-10g KH
2PO
45～10g MgSO
41～6g water 1L
Sterilization: 115 ℃, steam sterilization 20 minutes.
Inoculation: bacteria suspension is inserted in the culture medium of sterilization.
Cultivate: 25-32 ℃, cultivate 72-108h.
Good effect of the present invention is: the multiple beneficial microorganism is at amphimicrobian condition bottom fermentation, obtain multiple flora a large amount of cells and tunning, like multiple enzyme, organic acid, small-molecular peptides, vitamin, oligosaccharide substance.These cells and material can suppress the discharging of ammonia, and the blocking-up scatol produces, and suppress the putrefaction bacteria growth, effectively noxious substances such as removal of hydrogen sulfide, nitrite, methane, indoles.
Compared with prior art: the present invention is the compound culture with the multiple beneficial microorganism, and under anaerobic fermentation obtains a large amount of multi-strain fermentation products; It is many to make it have a microbe groups; Function is many, and metabolite is many, effectively the characteristics of stable application effect.
The specific embodiment
Instance 1.
1) will be used for the strain that deep layer leaves standstill cultivation, respectively activation in the sterilization bottle.
Leave standstill the fluid medium of cultivation, its component is: sucrose 10～30g peptone 4～10g yeast extract 5-10g KH
2PO
45～10g MgSO
41～6g water 1L
2) above-mentioned cultivation is based on 115 ℃, steam sterilization 20 minutes.
3) activatory bacteria suspension is inserted in the culture medium, 25-37 ℃, cultivated 3-7 days.
This product dilution 500-1000 doubly to the sterilization of fowl carcass external spraying, can purify the air of colony house, eliminates self stink, reduces diseases of bird and livestock and takes place.
Instance 2.
1) will be used for the strain that deep layer leaves standstill cultivation, respectively activation in the sterilization bottle.
2) leave standstill the fluid medium of cultivation, its component is: sucrose 10～30g peptone 4-10g yeast extract 5-10gKH
2PO
45～10gMgSO
41～6g water 1L.
3) above-mentioned cultivation is based on 115 ℃, steam sterilization 20 minutes.
4) activatory bacteria suspension is inserted in the culture medium, 25-37 ℃, cultivated 3-7 days.
5) with the solid carrier mix homogeneously, the fermentation of banking up, 36h.
6) drying and crushing is solid product.
This product carries out compost by 0.5% inoculum concentration, can accelerate to become thoroughly decomposed the time.Under the general room temperature, banked up 20 days, can fundamentally improve large-scale plant internal and external environment, can also promote muck unreal day.
The liquor product with 1 kilogram of diluent, sprays 1 mu of water surface, can remove the pond abnormal flavour, and spray in every month purifies water for 1-3 time, also can be used for aquaculture, and the effect of clean-up oxygenation is arranged.
Behind the application 1 month, the stench of plant's fowl and animal excrement has been eliminated, and ammonia emission reduces to 250PPM, colony house stench lowering of concentration 97.7%, the domestic animal asthma that causes therefrom and the sickness rate of poultry commonly encountered diseases have also reduced.The hygienic conditions of culturing have changed, and mosquitos and flies reduces gradually and even disappears.
Claims (6)
1. the method for preparing of a compound bio deodorizer; Comprise the preparation of function strain; It is characterized in that taking purebred cultivation according to the physiological characteristics of strain; Carry out compoundly then according to a certain percentage, composite flora obtains the living bacterial cells and the metabolite of maximum quantity through amphimicrobian fermentation culture mode.
2. the method for preparing of a kind of compound bio deodorizer according to claim 1; It is characterized in that amphimicrobian fermentation culture mode; Adopt deep liquid to leave standstill cultivation; Needs according to each strain are selected appropriate media, mainly are under anaerobic to make its fermentation, obtain the tunning of maximum.
3. the method for preparing of a kind of compound bio deodorizer according to claim 1; The microorganism that comprises bacillus, streptomyces, Saccharomycodes, genus lactubacillus, acetic acid Pseudomonas etc. is adopted in the preparation that it is characterized in that the function strain; Have various active enzyme and secondary metabolite, like multiple organic acid, small-molecular peptides, vitamin, oligosaccharide substance.
4. the method for preparing of a kind of compound bio deodorizer according to claim 3 is characterized in that having in the described culture medium of preparation of function strain the ratio of Semen sojae atricolor hydrolysate and each component: Semen sojae atricolor hydrolysate 10～50g peptone 4～10g sucrose 16～24g KH
2PO
43～8g MgSO
41～6g water 1L.
5. the method for preparing of a kind of compound bio deodorizer according to claim 2; The cultivation that it is characterized in that the probiotic bacteria strain adopts strain in vital movement; Can produce various active enzyme and secondary metabolite, like the microorganism of multiple organic acid, small-molecular peptides, vitamin, oligosaccharide substance.The anaerobism training method of selecting deep layer to leave standstill, difference or compound cultivation.Inoculum concentration proportioning: 1: 5-40.
6. the method for preparing of a kind of compound bio deodorizer according to claim 5 is characterized in that having following component and weight proportion in the anaerobic culture medium that deep layer leaves standstill: sucrose 10～30g peptone 4～10g yeast extract 5-10g KH
2PO
45～10g MgSO
41～6g water 1L.
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| CN2011101501140A CN102813948A (en) | 2011-06-07 | 2011-06-07 | Preparation method of composite biological deodorant |
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| CN2011101501140A CN102813948A (en) | 2011-06-07 | 2011-06-07 | Preparation method of composite biological deodorant |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103041423A (en) * | 2012-12-26 | 2013-04-17 | 福建大用生态农业综合发展有限公司 | Water lettuce deodorant |
| CN103749942A (en) * | 2013-12-31 | 2014-04-30 | 陕西万源生物农业科技有限公司 | Compound probiotics for animal breeding |
| CN105000675A (en) * | 2015-07-13 | 2015-10-28 | 上海新张卫生用品有限公司 | Method for controlling toilet odor with microbial agent |
| CN105032169A (en) * | 2015-07-22 | 2015-11-11 | 江西美丽家居生态环保有限公司 | Production method for microorganism metabolite organic formaldehyde removing agent |
| CN105779335A (en) * | 2016-03-15 | 2016-07-20 | 甘肃省农业科学院生物技术研究所 | Formula and preparation method of micro-ecological deodorant |
| CN106110363A (en) * | 2016-08-16 | 2016-11-16 | 叶海霞 | A kind of biological deodorant being applied to public lavatory |
| CN107581621A (en) * | 2017-10-14 | 2018-01-16 | 刘荣甫 | A kind of healthy food of the Phellinus ganoderma lucidum mycelium combination small-molecular peptides made of selenium malt |
| CN107617331A (en) * | 2017-10-10 | 2018-01-23 | 默克环保科技（湖南）有限公司 | A kind of environment friendly biological deodorant and preparation method thereof |
| CN115261285A (en) * | 2022-09-01 | 2022-11-01 | 浙江科技学院 | Preparation method and equipment of biological full-degradation kitchen garbage FDM (fused deposition modeling) microbial inoculum |
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| CN101775356A (en) * | 2009-12-08 | 2010-07-14 | 营口恒新生物技术开发有限公司 | Complex microorganism fungicide for treating animal manure and preparation method and application thereof |
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| CN1248622A (en) * | 1999-09-27 | 2000-03-29 | 李开义 | Compound microecological preparation |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103041423A (en) * | 2012-12-26 | 2013-04-17 | 福建大用生态农业综合发展有限公司 | Water lettuce deodorant |
| CN103041423B (en) * | 2012-12-26 | 2014-10-22 | 福建大用生态农业综合发展有限公司 | Water lettuce deodorant |
| CN103749942A (en) * | 2013-12-31 | 2014-04-30 | 陕西万源生物农业科技有限公司 | Compound probiotics for animal breeding |
| CN105000675A (en) * | 2015-07-13 | 2015-10-28 | 上海新张卫生用品有限公司 | Method for controlling toilet odor with microbial agent |
| CN105032169A (en) * | 2015-07-22 | 2015-11-11 | 江西美丽家居生态环保有限公司 | Production method for microorganism metabolite organic formaldehyde removing agent |
| CN105779335A (en) * | 2016-03-15 | 2016-07-20 | 甘肃省农业科学院生物技术研究所 | Formula and preparation method of micro-ecological deodorant |
| CN106110363A (en) * | 2016-08-16 | 2016-11-16 | 叶海霞 | A kind of biological deodorant being applied to public lavatory |
| CN107617331A (en) * | 2017-10-10 | 2018-01-23 | 默克环保科技（湖南）有限公司 | A kind of environment friendly biological deodorant and preparation method thereof |
| CN107581621A (en) * | 2017-10-14 | 2018-01-16 | 刘荣甫 | A kind of healthy food of the Phellinus ganoderma lucidum mycelium combination small-molecular peptides made of selenium malt |
| CN115261285A (en) * | 2022-09-01 | 2022-11-01 | 浙江科技学院 | Preparation method and equipment of biological full-degradation kitchen garbage FDM (fused deposition modeling) microbial inoculum |
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Application publication date: 20121212 |