CN105779335A - Formula and preparation method of micro-ecological deodorant - Google Patents
Formula and preparation method of micro-ecological deodorant Download PDFInfo
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- CN105779335A CN105779335A CN201610145393.4A CN201610145393A CN105779335A CN 105779335 A CN105779335 A CN 105779335A CN 201610145393 A CN201610145393 A CN 201610145393A CN 105779335 A CN105779335 A CN 105779335A
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Abstract
The invention belongs to the technical field of microbiological deodorants, and particularly relates to a formula and a preparation method of a micro-ecological deodorant produced by using potato starch waste water. The micro-ecological deodorant is prepared from the following components: the potato starch waste water, yeast powder, albumen powder, cane sugar, glucose, magnesium sulphate, sodium chloride, sodium alginate and biological microbial inoculum, wherein the biological microbial inoculum includes bacillus, photosynthetic bacteria, lactic acid bacteria, microzyme and actinomycetes. According to the formula and the preparation method disclosed by the invention, the biological microbial inoculum is cultured, the multiplication rate is high, the yield is large, the production cycle is short, the energy consumption is low, the growing conditions are controllable, besides, mass production can be realized, and the conflict between the requirements on raw materials of the microbial inoculum and high cost of the production of conventional microbial inocula in the industrialized production of the micro-ecological deodorant is effectively solved.
Description
Technical field
The invention belongs to microbial deodorant technical field, particularly a kind of Tiny ecosystem deodorizer formula using potato starch wastewater to produce and preparation method thereof.
Background technology
Microbial deodorant method be the metabolism utilizing microorganism by odorant, be converted into the cellular material of microorganism, or be degraded to water and carbon dioxide;Biological deodorizing effect is moderately good, wide adaptability, and expense is relatively low.Needed for biological deodorizing, strain and nutritional solution are all natural prodcuts, and operator and environment are not resulted in any negative effect, more will not cause any secondary pollution directly or indirectly.At present, although Tiny ecosystem deodorizer is widely applied, but it cultivates the preparation of raw material many employings chemical agent, and large-scale production is relatively costly.Tiny ecosystem deodorizer has fast growth, the expanding propagation cycle is short, breeding potential is high, indoor propagation condition is controlled, can not be subject to seasonal restrictions the features such as anniversary cultivation to utilize potato starch wastewater to cultivate biological bacterial strain technology production.Current document yet there are no about the research report utilizing potato starch wastewater to cultivate biological bacterial strain large-scale production Tiny ecosystem deodorizer.
Summary of the invention
It is an object of the invention to provide a kind of Tiny ecosystem deodorizer formula using potato starch wastewater to produce and preparation method thereof, the method can produce substantial amounts of Tiny ecosystem deodorizer within a short period of time, the problem being solved Tiny ecosystem deodorizer inadequate resource by animal nutrition, provides sufficient bacteria agent raw material for industrialized production Tiny ecosystem deodorizer.
This invention address that its technical problem adopts the following technical scheme that
A kind of Tiny ecosystem deodorizer formula, including following component: potato starch wastewater, yeast powder, egg albumen powder, sucrose, glucose, magnesium sulfate, sodium chloride, sodium alginate and bacteria agent, bacteria agent includes bacillus cereus, photosynthetic bacteria, lactic acid bacteria, yeast and actinomycetes;
Using the potato starch wastewater after sterilizing as culture medium, culture medium is added sucrose 10.0~20.0g/L, glucose 0.1~10.0g/L, egg albumen powder 0.1~0.5g/L, yeast powder 0.1~0.5g/L, magnesium sulfate 0.1~0.2g/L, sodium chloride 1.0~5.0g/L and sodium alginate 0.1~0.5g/L respectively;Using mass concentration 0.1~2.0%NaOH solution to adjust culture medium solution pH value is 6.0~6.2;Use steam sterilizing 20.0 ~ 30.0 minutes under pressure 0.10~0.15MPa;Culture medium adds bacteria agent, every kind of strain 0.1~0.5g/L in bacteria agent after being cooled to room temperature.
Described bacteria agent includes antibacterial culturing based formulas and fungal culture based formulas:
Antibacterial culturing based formulas is:
Sucrose 10.0~20.0g/L, glucose 0.1~10.0g/L, egg albumen powder 0.1~0.5g/L, yeast powder 0.1~0.5g/L, sodium chloride 1.0~5.0g/L and sodium alginate 0.1~0.5g/L;Bacillus cereus 0.1 ~ 0.5g/L, photosynthetic bacteria 0.2 ~ 0.3g/L, lactic acid bacteria 0.1 ~ 0.2g/L in bacteria agent.
Fungal culture based formulas is:
Sucrose 15.0~20.0g/L, glucose 2.0~10.0g/L, yeast powder 0.1~0.5g/L magnesium sulfate 0.1~0.2g/L and sodium alginate 0.1~0.5g/L;Yeast 0.1 ~ 0.5g/L, actinomycetes 0.1 ~ 0.2g/L in bacteria agent.
Comprise the steps:
A, potato starch wastewater sterilizing
By potato starch wastewater by duct type ultraviolet sterilization device sterilizing 30 ~ 40min, send into aseptic stainless cylinder of steel and deposit;
B, medium sterilization
Bacteria culture media, fungi culture medium are heat steam sterilization 20.0 ~ 30.0 minutes under 0.10~0.15MPa at pressure, are cooled to room temperature standby;
C, antibacterial microbial inoculum are cultivated
Take test tube kind bacillus cereus, photosynthetic bacteria, lactic acid bacteria respectively, the superclean bench of cleanliness factor after sterilization 100 grades is inoculated, 28.0~33.0 DEG C of shaking tables are cultivated, shakes continuously every day 12.0~24.0 hours, cultivate 5~7 days;Light and antibacterial need to add illumination 1000~2000LX;
D, fungal inoculant are cultivated
Take test tube Yeasts, actinomycetes respectively, the superclean bench of cleanliness factor after sterilization 100 grades is inoculated, 28.0~33.0 DEG C of shaking tables are cultivated, shakes continuously every day 12.0~24.0 hours, cultivate 5~7 days;
E, microbial inoculum compound
Aseptically, in the superclean bench that cleanliness factor is 100 grades, respectively the strain that step C and D cultivates being mixed into Tiny ecosystem deodorizer, mixed proportion is bacillus cereus 0.1 ~ 0.5g/L, photosynthetic bacteria 0.2 ~ 1.5g/L, lactic acid bacteria 0.1 ~ 2.0g/L, yeast 0.1 ~ 0.5g/L, actinomycetes 0.1 ~ 0.6g/L.
F, microbial inoculum are preserved
The Tiny ecosystem deodorizer mixed is according to different size plastic bottle fillings such as product 250ml, 500ml, 1000ml, 5L, and shady and cool dry place stores, and the shelf-life is 18 months.
In described step B, bacteria culture media, fungi culture medium are divided in 100ml triangle culture bottle respectively, every bottle of 20.0~25.0ml, bottle lid breathable plastic sealed membrane, is heat steam sterilization 20.0 ~ 30.0 minutes under 0.10~0.15MPa at pressure, is cooled to room temperature standby.
The invention have the benefit that
1, this formula adds potato starch wastewater and replaces partially protein, adds sodium chloride, better meets the different bacterium strain tissue fast-growth needs to protein, promotes thalline tissue Fast-propagation.Reduce the addition of egg albumen powder reagent in culture medium, reduce culture medium cost.
2, this formula adds potato starch wastewater and replaces part trace element, riboflavin, adds magnesium sulfate, better meets the different fungus strain tissue fast-growth needs to trace element and riboflavin, promotes thalline tissue Fast-propagation.The addition of supplementing culture medium medium trace element and riboflavin, reduces culture medium cost.
Tiny ecosystem deodorizer is by photosynthetic bacteria, lactic acid bacteria, yeast, acetic acid bacteria, bacillus bifidus, actinomycetes, the bacteria agent that in the big quasi-microorganism of bacillus cereus 5, multiple beneficial microorganism is composited.Every milliliter containing living bacteria count >=1,000,000,000 cfu.After this bacteria agent mixes feedstuff and drinking-water entrance animal intestinal, strong dominant population is collectively formed with probiotics in intestinal, suppress and eliminate pathogenic flora, secretion simultaneously and a large amount of aminoacid of synthesis, protein, vitamin, various biochemical enzymes, antibiotic, somatomedin etc. nutrition and hormonal substance, to adjust and to improve each organ dysfunction of poultry body, improve food conversion ratio, poultry are produced immunity, nutrition, the multiple effect such as growth stimulation, reach to prevent and cure diseases, improve survival rate, promote growth and breeding, reduce cost, eliminate fecaluria stink, environment purification, the positive effects such as increasing both production and income.The foul gas such as hydrogen sulfide, ammonia can effectively be removed by this Tiny ecosystem deodorizer, deodorization rate and press down fly rate and reach more than 70%;Significantly reduce the content of COD and ammonia nitrogen in sewage, strengthen purification speed and the ability of sewage, human body and animals and plants is without any side effects, environment is not produced any pollution.
China's potato starch wastewater amount reaches 8,000,000 tons, utilization rate is less than 10%, owing to potato waste water containing a lot of nutritional labelings, such as protein 2.3%, fat 1.1%, thiamine 0.07mg, riboflavin 0.11mg, nicotinic acid 1.1mg, a small amount of carotene and ascorbic acid.Waste water can as the good culture fluid of Tiny ecosystem deodorizer after sterilization treatment.Improve the value of waste water, solve discharge of wastewater environmental pollution problem.
The present invention is adopted to cultivate bacteria agent in sum, growth rate is fast, yield is big, with short production cycle, power consumption is few, growth conditions is controlled, and it is capable of large-scale production, effectively solve Tiny ecosystem deodorizer industrialized production and microbial inoculum raw materials requirement and conventional microbial inoculum are produced the contradiction between high cost.
Detailed description of the invention
A kind of Tiny ecosystem deodorizer formula, it is characterized in that including following component: potato starch wastewater, yeast powder, egg albumen powder, sucrose, glucose, magnesium sulfate, sodium chloride, sodium alginate and bacteria agent, bacteria agent includes bacillus cereus, photosynthetic bacteria, lactic acid bacteria, yeast and actinomycetes;
Using the potato starch wastewater after sterilizing as culture medium, culture medium is added sucrose 10.0~20.0g/L, glucose 0.1~10.0g/L, egg albumen powder 0.1~0.5g/L, yeast powder 0.1~0.5g/L, magnesium sulfate 0.1~0.2g/L, sodium chloride 1.0~5.0g/L and sodium alginate 0.1~0.5g/L respectively;Using mass concentration 0.1~2.0%NaOH solution to adjust culture medium solution pH value is 6.0~6.2;Use steam sterilizing 20.0 ~ 30.0 minutes under pressure 0.10~0.15MPa;Culture medium adds bacteria agent, every kind of strain 0.1~0.5g/L in bacteria agent after being cooled to room temperature.
Described bacteria agent includes antibacterial culturing based formulas and fungal culture based formulas:
Antibacterial culturing based formulas is:
Sucrose 10.0~20.0g/L, glucose 0.1~10.0g/L, egg albumen powder 0.1~0.5g/L, yeast powder 0.1~0.5g/L, sodium chloride 1.0~5.0g/L and sodium alginate 0.1~0.5g/L;Bacillus cereus 0.1 ~ 0.5g/L, photosynthetic bacteria 0.2 ~ 0.3g/L, lactic acid bacteria 0.1 ~ 0.2g/L in bacteria agent.
Fungal culture based formulas is:
Sucrose 15.0~20.0g/L, glucose 2.0~10.0g/L, yeast powder 0.1~0.5g/L magnesium sulfate 0.1~0.2g/L and sodium alginate 0.1~0.5g/L;Yeast 0.1 ~ 0.5g/L, actinomycetes 0.1 ~ 0.2g/L in bacteria agent.
The preparation method of a kind of Tiny ecosystem deodorizer formula, it is characterised in that comprise the steps:
A, potato starch wastewater sterilizing
By potato starch wastewater by duct type ultraviolet sterilization device sterilizing 30 ~ 40min, send into aseptic stainless cylinder of steel and deposit;
B, medium sterilization
Bacteria culture media, fungi culture medium are divided in 100ml triangle culture bottle respectively, every bottle of 20.0~25.0ml, bottle lid breathable plastic sealed membrane, are heat steam sterilization 20.0 ~ 30.0 minutes under 0.10~0.15MPa at pressure, are cooled to room temperature standby;
C, antibacterial microbial inoculum are cultivated
Take test tube kind bacillus cereus, photosynthetic bacteria, lactic acid bacteria respectively, the superclean bench of cleanliness factor after sterilization 100 grades is inoculated, 28.0~33.0 DEG C of shaking tables are cultivated, shakes continuously every day 12.0~24.0 hours, cultivate 5~7 days;Light and antibacterial need to add illumination 1000~2000LX;
D, fungal inoculant are cultivated
Take test tube Yeasts, actinomycetes respectively, the superclean bench of cleanliness factor after sterilization 100 grades is inoculated, 28.0~33.0 DEG C of shaking tables are cultivated, shakes continuously every day 12.0~24.0 hours, cultivate 5~7 days;
E, microbial inoculum compound
Aseptically, in the superclean bench that cleanliness factor is 100 grades, respectively the strain that step C and D cultivates being mixed into Tiny ecosystem deodorizer, mixed proportion is bacillus cereus 0.1 ~ 0.5g/L, photosynthetic bacteria 0.2 ~ 1.5g/L, lactic acid bacteria 0.1 ~ 2.0g/L, yeast 0.1 ~ 0.5g/L, actinomycetes 0.1 ~ 0.6g/L.
F, microbial inoculum are preserved
The Tiny ecosystem deodorizer mixed is according to different size plastic bottle fillings such as product 250ml, 500ml, 1000ml, 5L, and shady and cool dry place stores, and the shelf-life is 18 months.
Embodiment 1:
A kind of potato starch wastewater is that substrate produces Tiny ecosystem deodorizer formula and preparation method thereof, it is characterised in that comprise the steps:
A, potato starch wastewater sterilizing:
By collecting the potato starch wastewater of coming, by duct type ultraviolet sterilization device sterilizing 40min, send into aseptic stainless cylinder of steel and deposit.
B, medium sterilization:
Bacteria culture media, fungi culture medium are divided in 100ml triangle culture bottle respectively, every bottle of 20.0ml, and bottle lid breathable plastic sealed membrane is sterilizing 30.0 minutes under 0.10MPa at pressure, cools down standby;
C, antibacterial microbial inoculum are cultivated:
Taking test tube kind bacillus cereus, photosynthetic bacteria, lactic acid bacteria respectively, inoculate in superclean bench after sterilization, constant-temperature table is cultivated, cultivation temperature is 33.0 DEG C, shakes continuously every day 12.0 hours, cultivates 7 days;Light and antibacterial need to add illumination 2000LX.
D, fungal inoculant are cultivated:
Taking test tube Yeasts, actinomycetes respectively, inoculate in superclean bench after sterilization, constant-temperature table is cultivated, cultivation temperature is 28.0 DEG C, shakes continuously every day 20.0 hours, cultivates 7 days;
E, microbial inoculum compound: aseptically respectively by cultured different microbial inoculums, according to quality than bacillus cereus 0.1g/L, photosynthetic bacteria 0.2g/L, lactic acid bacteria 2.0g/L, yeast 0.1g/L, actinomycetes 0.1g/L, be mixed into Tiny ecosystem deodorizer.Deodorization rate reaches 86.5%.
F, microbial inoculum are preserved:
Mixed Tiny ecosystem deodorizer is according to product different size fill, and shady and cool dry place stores, and the shelf-life is 18 months.
Embodiment 2:
A kind of potato starch wastewater is that substrate produces Tiny ecosystem deodorizer formula and preparation method thereof, it is characterised in that comprise the steps:
A, potato starch wastewater sterilizing:
By collecting the potato starch wastewater of coming, by duct type ultraviolet sterilization device sterilizing 35min, send into aseptic stainless cylinder of steel and deposit.
B, medium sterilization:
Bacteria culture media, fungi culture medium are divided in 100ml triangle culture bottle respectively, every bottle of 22.0ml, and bottle lid breathable plastic sealed membrane is sterilizing 25.0 minutes under 0.12MPa at pressure, cools down standby;
C, antibacterial microbial inoculum are cultivated:
Taking test tube kind bacillus cereus, photosynthetic bacteria, lactic acid bacteria respectively, inoculate in superclean bench after sterilization, constant-temperature table is cultivated, cultivation temperature is 30.0 DEG C, shakes continuously every day 18.0 hours, cultivates 6 days;Light and antibacterial need to add illumination 1000LX.
D, fungal inoculant are cultivated:
Taking test tube Yeasts, actinomycetes respectively, inoculate in superclean bench after sterilization, constant-temperature table is cultivated, cultivation temperature is 30.0 DEG C, shakes continuously every day 18.0 hours, cultivates 6 days;
E, microbial inoculum compound: aseptically respectively by cultured different microbial inoculums, according to quality than bacillus cereus 0.3g/L, photosynthetic bacteria 1.0g/L, lactic acid bacteria 1.0g/L, yeast 0.3g/L, actinomycetes 0.4g/L, be mixed into Tiny ecosystem deodorizer.Deodorization rate reaches 92.6%
F, microbial inoculum are preserved:
Mixed Tiny ecosystem deodorizer is according to product different size fill, and shady and cool dry place stores, and the shelf-life is 18 months.
Embodiment 3:
A kind of potato starch wastewater is that substrate produces Tiny ecosystem deodorizer formula and preparation method thereof, it is characterised in that comprise the steps:
A, potato starch wastewater sterilizing:
By collecting the potato starch wastewater of coming, by duct type ultraviolet sterilization device sterilizing 40min, send into aseptic stainless cylinder of steel and deposit.
B, medium sterilization:
Bacteria culture media, fungi culture medium are divided in 100ml triangle culture bottle respectively, every bottle of 25.0ml, and bottle lid breathable plastic sealed membrane is sterilizing 20.0 minutes under 0.15MPa at pressure, cools down standby;
C, antibacterial microbial inoculum are cultivated:
Taking test tube kind bacillus cereus, photosynthetic bacteria, lactic acid bacteria respectively, inoculate in superclean bench after sterilization, constant-temperature table is cultivated, cultivation temperature is 28.0 DEG C, shakes continuously every day 24.0 hours, cultivates 5 days;Light and antibacterial need to add illumination 1000LX.
D, fungal inoculant are cultivated:
Taking test tube Yeasts, actinomycetes respectively, inoculate in superclean bench after sterilization, constant-temperature table is cultivated, cultivation temperature is 32.0 DEG C, shakes continuously every day 10.0 hours, cultivates 5 days;
E, microbial inoculum compound: aseptically respectively by cultured different microbial inoculums, according to quality than bacillus cereus 0.5g/L, photosynthetic bacteria 1.5g/L, lactic acid bacteria 0.1g/L, yeast 0.5g/L, actinomycetes 0.6g/L, be mixed into Tiny ecosystem deodorizer.Deodorization rate reaches 96.2%.
F, microbial inoculum are preserved:
Mixed Tiny ecosystem deodorizer is according to product different size fill, and shady and cool dry place stores, and the shelf-life is 18 months.
Claims (5)
1. a Tiny ecosystem deodorizer formula, it is characterized in that including following component: potato starch wastewater, yeast powder, egg albumen powder, sucrose, glucose, magnesium sulfate, sodium chloride, sodium alginate and bacteria agent, bacteria agent includes bacillus cereus, photosynthetic bacteria, lactic acid bacteria, yeast and actinomycetes;
Using the potato starch wastewater after sterilizing as culture medium, culture medium is added sucrose 10.0~20.0g/L, glucose 0.1~10.0g/L, egg albumen powder 0.1~0.5g/L, yeast powder 0.1~0.5g/L, magnesium sulfate 0.1~0.2g/L, sodium chloride 1.0~5.0g/L and sodium alginate 0.1~0.5g/L respectively;Using mass concentration 0.1~2.0%NaOH solution to adjust culture medium solution pH value is 6.0~6.2;Use steam sterilizing 20.0 ~ 30.0 minutes under pressure 0.10~0.15MPa;Culture medium adds bacteria agent, every kind of strain 0.1~0.5g/L in bacteria agent after being cooled to room temperature.
2. a kind of Tiny ecosystem deodorizer formula according to claim 1, it is characterised in that described bacteria agent includes antibacterial culturing based formulas and fungal culture based formulas:
Antibacterial culturing based formulas is:
Sucrose 10.0~20.0g/L, glucose 0.1~10.0g/L, egg albumen powder 0.1~0.5g/L, yeast powder 0.1~0.5g/L, sodium chloride 1.0~5.0g/L and sodium alginate 0.1~0.5g/L;Bacillus cereus 0.1 ~ 0.5g/L, photosynthetic bacteria 0.2 ~ 0.3g/L, lactic acid bacteria 0.1 ~ 0.2g/L in bacteria agent;
Fungal culture based formulas is:
Sucrose 15.0~20.0g/L, glucose 2.0~10.0g/L, yeast powder 0.1~0.5g/L magnesium sulfate 0.1~0.2g/L and sodium alginate 0.1~0.5g/L;Yeast 0.1 ~ 0.5g/L, actinomycetes 0.1 ~ 0.2g/L in bacteria agent.
3. the preparation method of a kind of Tiny ecosystem deodorizer formula according to claim 1 and 2, it is characterised in that comprise the steps:
A, potato starch wastewater sterilizing
By potato starch wastewater by duct type ultraviolet sterilization device sterilizing 30 ~ 40min, send into aseptic stainless cylinder of steel and deposit;
B, medium sterilization
Bacteria culture media, fungi culture medium are heat steam sterilization 20.0 ~ 30.0 minutes under 0.10~0.15MPa at pressure, are cooled to room temperature standby;
C, antibacterial microbial inoculum are cultivated
Take test tube kind bacillus cereus, photosynthetic bacteria, lactic acid bacteria respectively, the superclean bench of cleanliness factor after sterilization 100 grades is inoculated, 28.0~33.0 DEG C of shaking tables are cultivated, shakes continuously every day 12.0~24.0 hours, cultivate 5~7 days;Light and antibacterial need to add illumination 1000~2000LX;
D, fungal inoculant are cultivated
Take test tube Yeasts, actinomycetes respectively, the superclean bench of cleanliness factor after sterilization 100 grades is inoculated, 28.0~33.0 DEG C of shaking tables are cultivated, shakes continuously every day 12.0~24.0 hours, cultivate 5~7 days;
E, microbial inoculum compound
Aseptically, in the superclean bench that cleanliness factor is 100 grades, respectively the strain that step C and D cultivates being mixed into Tiny ecosystem deodorizer, mixed proportion is bacillus cereus 0.1 ~ 0.5g/L, photosynthetic bacteria 0.2 ~ 1.5g/L, lactic acid bacteria 0.1 ~ 2.0g/L, yeast 0.1 ~ 0.5g/L, actinomycetes 0.1 ~ 0.6g/L.
4. the preparation method of a kind of Tiny ecosystem deodorizer formula according to claim 3, it is characterised in that also include
F, microbial inoculum are preserved
The Tiny ecosystem deodorizer mixed is according to different size plastic bottle fillings such as product 250ml, 500ml, 1000ml, 5L, and shady and cool dry place stores, and the shelf-life is 18 months.
5. the preparation method of a kind of Tiny ecosystem deodorizer formula according to claim 3, it is characterized in that in described step B, bacteria culture media, fungi culture medium are divided in 100ml triangle culture bottle respectively, every bottle of 20.0~25.0ml, bottle lid breathable plastic sealed membrane, it is heat steam sterilization 20.0 ~ 30.0 minutes under 0.10~0.15MPa at pressure, is cooled to room temperature standby.
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CN107647201A (en) * | 2017-11-28 | 2018-02-02 | 湖南军汇生物科技有限公司 | A kind of organic composite nutrient fish meal and its production method |
CN108085276A (en) * | 2017-12-26 | 2018-05-29 | 江苏星海生物科技有限公司 | For breeding pollution and the micro-ecological complex bacterial agent as enteron aisle improver and its application simultaneously |
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CN112725244A (en) * | 2021-02-25 | 2021-04-30 | 宁夏大学 | Efficient deodorant microbial inoculum for stink of potato starch wastewater |
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