CN108902448A - A kind of method of feces of livestock and poultry deodorization - Google Patents
A kind of method of feces of livestock and poultry deodorization Download PDFInfo
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- CN108902448A CN108902448A CN201810657762.7A CN201810657762A CN108902448A CN 108902448 A CN108902448 A CN 108902448A CN 201810657762 A CN201810657762 A CN 201810657762A CN 108902448 A CN108902448 A CN 108902448A
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- livestock
- poultry
- feces
- saccharomycete
- lactic acid
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- 244000144977 poultry Species 0.000 title claims abstract description 70
- 244000144972 livestock Species 0.000 title claims abstract description 68
- 210000003608 fece Anatomy 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000004332 deodorization Methods 0.000 title claims abstract description 21
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 78
- 241000894006 Bacteria Species 0.000 claims abstract description 44
- 241001481656 Stratiomyidae Species 0.000 claims abstract description 42
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 39
- 239000004310 lactic acid Substances 0.000 claims abstract description 39
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 36
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- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
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- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
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- NQPDZGIKBAWPEJ-UHFFFAOYSA-N valeric acid Chemical compound CCCCC(O)=O NQPDZGIKBAWPEJ-UHFFFAOYSA-N 0.000 description 2
- 240000001851 Artemisia dracunculus Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
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- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241000257159 Musca domestica Species 0.000 description 1
- 241000108664 Nitrobacteria Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000233805 Phoenix Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 241000254109 Tenebrio molitor Species 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
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- 238000010564 aerobic fermentation Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- ZRIUUUJAJJNDSS-UHFFFAOYSA-N ammonium phosphates Chemical compound [NH4+].[NH4+].[NH4+].[O-]P([O-])([O-])=O ZRIUUUJAJJNDSS-UHFFFAOYSA-N 0.000 description 1
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- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
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- 239000000835 fiber Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
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- 150000002576 ketones Chemical class 0.000 description 1
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- 150000002989 phenols Chemical class 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
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- YQUVCSBJEUQKSH-UHFFFAOYSA-N protochatechuic acid Natural products OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
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- 230000002000 scavenging effect Effects 0.000 description 1
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- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
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- 239000008223 sterile water Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 229940005605 valeric acid Drugs 0.000 description 1
- WKOLLVMJNQIZCI-UHFFFAOYSA-N vanillic acid Chemical compound COC1=CC(C(O)=O)=CC=C1O WKOLLVMJNQIZCI-UHFFFAOYSA-N 0.000 description 1
- TUUBOHWZSQXCSW-UHFFFAOYSA-N vanillic acid Natural products COC1=CC(O)=CC(C(O)=O)=C1 TUUBOHWZSQXCSW-UHFFFAOYSA-N 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Environmental Sciences (AREA)
- Sustainable Development (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Organic Chemistry (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of methods of feces of livestock and poultry deodorization; belong to livestock and poultry cultivation technical field, environmental protection technical field; it includes the domestication and expansion culture of saccharomycete and lactic acid bacteria; the hatching of black soldier flies ovum and the cultivation of larva, the method for the present invention handle feces of livestock and poultry using saccharomycete, lactic acid bacteria and black soldier flies；Achieve the effect that feces of livestock and poultry is quick, stablizes, long-acting deodorization.
Description
Technical field
The invention belongs to livestock and poultry cultivation technical fields, environmental protection technical field, are related to a kind of side of feces of livestock and poultry deodorization
Method.
Background technique
With the scale of China's livestock and poultry breeding industry, intensivization development, problem of environmental pollution is more serious, and management difficulty adds
Greatly.Stench can be generated in feces of livestock and poultry stacking process, lead to environmental degradation.The pig farm of one annual output, 10.8 ten thousand hogs, per small
Shi Kexiang atmosphere discharges 1,500,000,000 thallus, 159.0kg NH3, 14.5kg H2S causes adverse effect to environment.NH3And H2S can
To cause eye and respiratory disease, and reduce livestock and poultry resistance and immunity, metabolism degree weakens, production capacity decline, together
When threaten the mankind physical and mental health.
The stink odor that feces of livestock and poultry generates is complicated, can be generally divided into following four major class：First is that volatility sulfur-bearing chemical combination
Object, such as H2S、CH4S, thioether class, etc., mainly from microorganism to the reduction of sulfate and the metabolism of sulfur-containing amino acid；Two
It is nitrogenous compound, such as NH3, trimethylamine etc., mainly from microorganism to the deamination of protein and amino acid；Third is that indoles
Class and phenols, the decomposition from microorganism to phenylalanine, tyrosine；Fourth is that volatile fat acids, as acetic acid, propionic acid,
Butyric acid, valeric acid etc., from the decomposition of the deamination and carbohydrate of amino acid.Wherein, H2S and NH3It is the main composition of stink
Ingredient is commonly used to indicate the foul smell content of poultry house.
Currently, feces of livestock and poultry odor treatment mainly has physical method, chemical method and bioanalysis three categories technology both at home and abroad.
Physical deodorization technology is to carry out temporary absorption using adsorbent and screening agent, cover stink and reach deodorization and imitate
Fruit, but deodorization purpose is fundamentally not achieved.Absorption method is to adsorb odorant using adsorbent, is primarily adapted for use in grease removal
The low concentrations such as fat acid, ammonia, alcohols and gas soluble easily in water, the active charcoal of common adsorbent, zeolite, silica gel, metal oxygen
Compound and large pore macromolecular material, there are the disadvantages of at high cost, adsorbance is limited.Masking method is to use the stronger substance of fragranced such as
Vanillic acid, piperonal, bavin sieve ketone etc. shelter stink, are suitable for the industries such as aquatic products, grease, leather and fiber process, cannot be from root
Deodorization and at high cost in sheet, using there is limitation.
Chemical deodorizing technology is to react to reach deodorization purpose, including oxygen by addition chemical substance and odorant
Change method, neutralisation, bactericidal assay etc..Oxidizing process is that reproducibility odorant is oxidized to harmless odorless substance using strong oxidizer
Process, common oxidant such as ozone, ferrate etc..Neutralisation is that one layer of calcium superphosphate is spread on poultry house padding, crosses phosphorus
The ammonia generated in sour calcium and excrement reacts, and generates tasteless solid phosphoric acid ammonium salt, can be reduced ammonia in excrement and distributes, and reduces
Ammonia concentration.Bactericidal assay is to spray padding using chemical substances such as hydrogen peroxide, potassium permanganate, copper sulphate, acetic acid, benzoic acid,
By sterilizing, inhibit the activity of harmful bacteria, reach inhibition and reduces the generation of harmful gas in livestock house.Chemical method removes
Smelly effect is good, but there are at high cost, reaction is complicated, have the shortcomings that secondary pollution, raising dung recovery utilizes difficulty, it is difficult to push away
Wide application.
Biological deodorization technology is the technology to grow up the fifties in last century, and the countries such as Japan, Germany, Holland achieve
Significant achievement.Bioanalysis is mainly so that odor pollutant is degraded to odorless, harmless whole production using the metabolism of microorganism itself
Object, such as CO2、H2O etc. reaches deodorization purpose.Biological deodorization is with high-efficient, without secondary pollution, equipment is simple, operating cost
The advantages that low, management service facilitates, it has also become administer one of major technique developing direction of stench.Currently used strain includes
Photosynthetic bacteria, nitrobacteria, denitrifying bacteria, bacillus, streptomycete, saccharomycete, lactic acid bacteria, aspergillus oryzae etc..Bioanalysis removes
It is smelly in practical applications problem is encountered that, work well within first 3~5 days, for a long time stack the later period still can distribute stench again,
The reason is that deodorizing microorganism there are bad adaptability, effect is single the disadvantages of, even if using combination microbial inoculum or a variety of enzymes are added
Preparation, what deodorizing effect was also difficult to avoid that is presented the trend being remarkably decreased at any time.Its basic reason is to send out with excrement
Ferment process flora changes therewith, and deodorizing microorganism substitutes death, and the odorant in excrement comes out again, especially
When static treatment excrement anaerobic fermentation.The effective ways for solving the problems, such as this at present are exactly to take aerobic fermentation i.e. fermentation bed
Technology can effectively improve the dissolved oxygen in excrement material by daily timing turning, avoid anaerobic fermentation to generate to a certain extent big
Foul gas is measured, but composting method lasts longer, at least needs more than 20 days, microbial decomposition causes largely to seek in composting process
Material damage is supported, simultaneously as still there is a certain amount of foul smell to generate in fermentation process, mating water spray is needed, photooxidation catalysis, lives
Property charcoal absorption etc. deodorizing devices, equipment investment and energy consumption greatly increase, the equipment of a small-sized fermentation bed is always put into close to 100
Ten thousand, cost is very high.
C, N organic nutrient substance is caused to be lost for microbiological treatment excrement, the halfway problem of Odor control, researcher
Sight insect has been invested.Dealing with earthworm method is one of the processing mode of organic solid castoff rather well received at present, in city
Has application in city's garbage disposal, wormcast also has certain deodorizing effect, but when temperature is higher than 35 DEG C, earthworm will be dead
It dies, if treatment temperature is not up to 60 DEG C, pathogen cannot be efficiently controlled, therefore organic solid castoff must be pre- by compost
Processing is innoxious to realize, especially contains the feces of livestock and poultry of a large amount of pathogenic microorganisms.In order to solve feces of livestock and poultry to peripheral ring
Border bring pressure, Miller are equal to the research for having started fly maggot processing excrement the 1970s, domestic and international a large number of researchers
Also correlative study work has been carried out, at present the enterprise of existing large-scale cultivation fly maggot processing excrement, but fly maggot breeding has production
Cost is excessively high, the drawbacks such as unhygienic, so the black soldier flies for not entering mankind room attract wide attention.Black soldier flies are a kind of money
Source property scavenging insect, larva have many advantages, such as that feeding habits are miscellaneous, heavy, resistance is strong, do not spread the disease, with fly maggot, yellow meal worm etc.
It is eponymous, it is known as " phoenix worm ", prepupa nutritive value is high, can be used as animal derived protein utilization.Moreover, black soldier flies can also
Play the role of inhibiting housefly by struggle for existence, inhibiting rate is up to 100%.It is particularly noteworthy that black soldier flies handle excrement
Just pathogen can be killed during, reduce foul smell discharge, there is the preferable value of environmental protection.Black soldier flies are existing for livestock and poultry cultivation
Patent and paper report " handle chicken manure, pig with black soldier flies as described in patent " a kind of cultural method of livestock and poultry cultivation zero-emission "
Excrement, three day old larvas that 10kg lactic acid bacteria is added in excrement per ton, 120g egg hatch goes out are mixed thoroughly ", because of the water content of freshly-slaughtered poultry excrement
Generally 75% or more, the water content of fresh pig dung is generally 90% or more, and water rushes excrement, the water content of bubble excrement is more up to 95%
More than, and the optimum moisture content of black soldier flies cultivation is 60%~80%, it is therefore necessary to it is greater than 80% animal dung to water content
Just the auxiliary materials such as sawdust, stalk, wheat bran are added and adjust water content, otherwise seriously affect the mobility and feeding ability of black soldier flies；
" lactic acid bacteria original seed is the wild compound lactobacillus strain of a generation that extraction is enriched with from wormwood plant ", plant origin
Lactic acid bacteria is often difficult to colonize and keep high vigor for a long time in feces of livestock and poultry, needs timing sprinkling supplement during the fermentation
Lactic acid bacteria.
Summary of the invention
The technical problem to be solved in the present invention is that providing a kind of method of feces of livestock and poultry deodorization, the method passes through micro- life
Object and black soldier flies are used cooperatively, and achieve the effect that feces of livestock and poultry is quick, stablizes, long-acting deodorization.
The present invention is achieved by the following technical solution：
A kind of method of feces of livestock and poultry deodorization, domestication and expansion culture including saccharomycete and lactic acid bacteria, black soldier flies ovum
The cultivation of hatching and larva handles feces of livestock and poultry using saccharomycete, lactic acid bacteria and black soldier flies.
The domestication and expansion culture of the saccharomycete and lactic acid bacteria：The saccharomycete DY1 for being isolated from feces of livestock and poultry is inoculated in
In feces of livestock and poultry leachate, 28~30 DEG C, 150r/min shaken cultivation 5 days, culture solution is taken to be inoculated in feces of livestock and poultry leaching again
In liquid, transfers 5 times repeatedly, take culture solution spare；The lactic acid bacteria LY2 for being isolated from feces of livestock and poultry is inoculated in feces of livestock and poultry leachate
In, 28~30 DEG C, stationary culture 5 days, culture solution is taken to be inoculated in feces of livestock and poultry leachate again, transfers 5 times repeatedly, take culture
Liquid is spare；
Above-mentioned saccharomycete DY1 and lactic acid bacteria LY2 are accessed into YPD culture simultaneously respectively with 0.5%~1% inoculum concentration of volume ratio
In base, 28~30 DEG C, for 24 hours, for 24 hours, saccharomycete is can be obtained in 35~37 DEG C of stationary cultures to 120~150r/min shaking table shaken cultivation
The culture solution of DY1 and lactic acid bacteria LY2；
(2) hatching of black soldier flies ovum and the cultivation of larva：Hatch black soldier flies worm's ovum and raises to 3 ages, obtained black soldier flies 3
Instar larvae；
(3) feces of livestock and poultry is handled using saccharomycete DY1, lactic acid bacteria LY2 and black soldier flies：It includes saw that feces of livestock and poultry, which is added,
Auxiliary material including end, wheat bran, rice husk, mushroom dreg, stalk is simultaneously mixed thoroughly, and water content mass ratio 50%~70% is adjusted；Every m3Excrement
The culture solution of 500~1000mL saccharomycete DY1 and lactic acid bacteria LY2 is added in material, mixes well, is laid in material pond, thickness of feed layer
5cm~10cm, according to every m3Excrement material 20~500,000 are inoculated with 3 instar larvae of black soldier flies, when 50% or more prepupa of black soldier flies
It ends processing, carries out the separation processing of excrement worm.
Further, the preparation method of the feces of livestock and poultry leachate：Fresh feces of livestock and poultry 100g is taken, 200mL water is added, sets
In 500mL triangular flask, 10~20 beades are added, 150r/min vibrates 30min, and then 5000r/min is centrifuged 10min,
Supernatant is taken, 121 DEG C of sterilizing 20min are cooling spare.
The beneficial effect of the present invention compared with prior art：
(1) pass through and add auxiliary material in right amount, adjust feces of livestock and poultry water content and carbon-nitrogen ratio, make its loose ventilative, nutrition equilibrium,
Microbial reproduction is not only suitable for utilize and meet black soldier flies vegetative activity needs；(2) aerobic is respectively obtained by domestication
The lactic acid bacteria LY2 of saccharomycete DY1 and anaerobic type, saccharomycete DY1 can not only deodorization and also generate active material be lactic acid bacteria LY2 mention
Promote to be proliferated for nutrition, lactic acid bacteria LY2, which generates lactic acid, can effectively inhibit the harmful microorganism in feces of livestock and poultry, and it is rotten to reduce organic matter
Lose decomposition release foul smell；(3) excrement material is become loose ventilative by the activity of black soldier flies, is not required to mechanical stirring and is saved equipment investment, drop
Low energy consumption, excrement material structure is conducive to saccharomycete DY1 proliferation, while black soldier flies itself also have deodorization, sterilization, drive fly effect, according to
50% or so feces of livestock and poultry is converted high protein and other nutritional ingredients by the feeding ability powerful by its, can be used for cultivating feeding
Material, frass then can be used as organic fertilizer use.
Specific embodiment
Deamination deodoriging properties compare after 1 saccharomycete of embodiment and lactobacillus tame
Saccharomycete DY1, DW1, XY1, XW1 for being isolated from feces of livestock and poultry are inoculated in respectively and soaked equipped with 100mL feces of livestock and poultry
Out in the 500mL triangular flask of liquid, 30 DEG C, 150r/min shaken cultivation 5 days, takes 10mL to be inoculated in above-mentioned feces of livestock and poultry again and leach
In liquid, transfers 5 times repeatedly, take culture solution spare.Lactic acid bacteria LY1, LY2 for being isolated from feces of livestock and poultry are inoculated in equipped with 100mL
In the 500mL triangular flask of feces of livestock and poultry leachate, 30 DEG C stationary culture 5 days, take 10mL to be inoculated in the leaching of above-mentioned feces of livestock and poultry again
Out in liquid, transfers 5 times repeatedly, take culture solution spare.Saccharomycete DY1, DW1, XY1, XW1 and lactic acid bacteria LY1, LY2 are taken respectively
Then culture solution 1mL access places 1 and 20mL is housed equipped in the gas-impermeable plastics box of 500g fresh chicken manure in each plastic casing
For the small beaker of 1% boric acid for absorbing the ammonia that chicken manure releases, control group accesses sterile water 1mL in chicken manure.Each processing
It does 3 in parallel, is placed in constant temperature incubation in 30 DEG C of incubators, the ammonia content in boric acid absorbing liquid is measured after 72h, measurement uses Na Shi
Reagent spectrophotometry calculates according to formula (1) and removes ammonia rate.
Except ammonia rate (%)=(control group ammonia-nitrogen content-test group ammonia-nitrogen content)/control group ammonia-nitrogen content
* 100% (1)
Deamination deodoriging properties compare after 1 saccharomycete of table and lactobacillus tame
Embodiment 2 utilizes saccharomycete, the Contrast on effect of lactic acid bacteria and black soldier flies processing feces of livestock and poultry
Wheat bran is added in feces of livestock and poultry and is mixed thoroughly, water content 60% is adjusted, is divided into 8 groups, is respectively connected to：(A) yeast
Bacterium DY1, (B) lactic acid bacteria LY2, (C) black soldier flies, (D) saccharomycete DY1+ lactic acid bacteria LY2, (E) saccharomycete DY1+ black soldier flies, (F) cream
Sour bacterium LY2+ black soldier flies, (G) saccharomycete DY1+ lactic acid bacteria LY2+ black soldier flies, (H) blank control, bacterium solution additional amount are every m3Excrement material
1000mL is added, mixes well, is laid in material pond, thickness of feed layer 5cm, the access amount of 3 instar larvae of black soldier flies is according to every m3Excrement
Expect 500,000 inoculation, respectively on day 3, the 8th day, the 13rd day, the 18th day sample, with sense organ divide stink grade, according to not smelly
" M0 ", micro- smelly " M1 ", more smelly " M2 ", very smelly " M3 ", stench " M4 " are divided into 4 grades, as a result as shown in table 2 below：
2 stink grade classification of table
Embodiment 3
A kind of method of feces of livestock and poultry deodorization, includes the following steps：
(1) domestication and expansion culture of saccharomycete and lactic acid bacteria：Saccharomycete DY1 (bacterial strain of feces of livestock and poultry will be isolated from
It is isolated and cultured by Ludong University Chen Guozhong teacher, those skilled in the art can be to old when implementing technical solution of the present invention
State loyalty teacher purchase) it is inoculated in the 500mL triangular flask equipped with 100mL feces of livestock and poultry leachate, 30 DEG C, 150r/min oscillation training
It supports 5 days, 10mL is taken to be inoculated in above-mentioned feces of livestock and poultry leachate again, transfer 5 times repeatedly, take culture solution spare.It will be isolated from
(bacterial strain is isolated and cultured the lactic acid bacteria LY2 of feces of livestock and poultry by Ludong University Chen Guozhong teacher, and those skilled in the art are in reality
Can be bought to Chen Guozhong teacher when applying technical solution of the present invention) it is inoculated in the 500mL tri- equipped with 100mL feces of livestock and poultry leachate
In the bottle of angle, 30 DEG C, stationary culture 5 days, 10mL is taken to be inoculated in above-mentioned feces of livestock and poultry leachate again, transfers 5 times repeatedly, take training
Nutrient solution is spare.Above-mentioned saccharomycete DY1 and lactic acid bacteria LY2 is accessed in YPD culture medium simultaneously with 0.5% inoculum concentration, 30 DEG C,
For 24 hours, 37 DEG C of stationary cultures for 24 hours, obtain the culture solution of saccharomycete DY1 and lactic acid bacteria LY2 to 150r/min shaking table shaken cultivation.
Feces of livestock and poultry leachate：Fresh feces of livestock and poultry 100g is taken, 200mL water is added, is placed in 500mL triangular flask, is added
10~20 beades, 150r/min vibrate 30min, and then 5000r/min is centrifuged 10min, take supernatant, 121 DEG C of sterilizings
20min, cooling are spare.
(2) hatching of black soldier flies ovum and the cultivation of larva：After black soldier flies worm's ovum is placed in 30 DEG C of hatchings 3 days, with dregs of beans and wheat
Bran formula feed, 30 DEG C, 80% >=humidity >=60% feeds 7 days, obtains 3 instar larvae of black soldier flies.
(3) feces of livestock and poultry is handled using saccharomycete, lactic acid bacteria and black soldier flies：Wheat bran is added in feces of livestock and poultry and is mixed thoroughly, is adjusted
Whole water content 60%, every m3The culture solution of 1000mL saccharomycete DY1 and lactic acid bacteria LY2 is added in excrement material, mixes well, is laid in material
Chi Zhong, thickness of feed layer 5cm, according to every m3500,000 inoculation 3 instar larvaes of black soldier flies of excrement material, 18 days 50% prepupa of black soldier flies, excrement worm
Sieve working process.Pass through detection, compared with blank, NH3And H2The removal rate of S respectively reaches 94.93% and 87.34%, and
Excrement is stirred without machinery in fermentation process.NH is measured using Berthelot spectrophotometry3Burst size, using zinc amine complex salt
Absorptiometry measures H2The burst size of S.
Claims (2)
1. a kind of method of feces of livestock and poultry deodorization, it is characterised in that it includes the domestication and expansion culture of saccharomycete and lactic acid bacteria,
The hatching of black soldier flies ovum and the cultivation of larva handle feces of livestock and poultry using saccharomycete, lactic acid bacteria and black soldier flies；
The domestication and expansion culture of the saccharomycete and lactic acid bacteria：The saccharomycete DY1 for being isolated from feces of livestock and poultry is inoculated in livestock and poultry
In excrement leachate, 28~30 DEG C, 150r/min shaken cultivation 5 days, culture solution is taken to be inoculated in feces of livestock and poultry leachate again,
It transfers 5 times repeatedly, takes culture solution spare；The lactic acid bacteria LY2 for being isolated from feces of livestock and poultry is inoculated in feces of livestock and poultry leachate, 28
~30 DEG C, stationary culture 5 days, culture solution is taken to be inoculated in feces of livestock and poultry leachate again, transfers 5 times repeatedly, take culture solution standby
With；
Above-mentioned saccharomycete DY1 and lactic acid bacteria LY2 are accessed into YPD culture medium respectively with 0.5%~1% inoculum concentration of volume ratio simultaneously
In, 28~30 DEG C, for 24 hours, for 24 hours, saccharomycete can be obtained in 35~37 DEG C of stationary cultures to 120~150r/min shaking table shaken cultivation
The culture solution of DY1 and lactic acid bacteria LY2；
(2) hatching of black soldier flies ovum and the cultivation of larva：Hatch black soldier flies worm's ovum and raises 3 age of the black soldier flies children obtained to 3 ages
Worm；
(3) feces of livestock and poultry is handled using saccharomycete DY1, lactic acid bacteria LY2 and black soldier flies：It includes sawdust, wheat that feces of livestock and poultry, which is added,
Auxiliary material including bran, rice husk, mushroom dreg, stalk is simultaneously mixed thoroughly, and water content mass ratio 50%~70% is adjusted；Every m3Excrement material is added
The culture solution of 500~1000mL saccharomycete DY1 and lactic acid bacteria LY2, mix well, and are laid in material pond, and thickness of feed layer 5cm~
10cm, according to every m3Excrement material 20~500,000 are inoculated with 3 instar larvae of black soldier flies, can terminate to locate when 50% or more prepupa of black soldier flies
Reason carries out the separation processing of excrement worm.
2. a kind of method of feces of livestock and poultry deodorization according to claim 1, it is characterised in that the feces of livestock and poultry leachate
Preparation method：Fresh feces of livestock and poultry 100g is taken, 200mL water is added, is placed in 500mL triangular flask, 10~20 glass are added
Pearl, 150r/min vibrate 30min, and then 5000r/min is centrifuged 10min, take supernatant, and 121 DEG C of sterilizing 20min are cooling spare.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110074007A (en) * | 2019-05-14 | 2019-08-02 | 广州无两生物科技有限公司 | A kind of poultry excrement and urine disposal method |
| CN113493288A (en) * | 2021-06-02 | 2021-10-12 | 广东省农业科学院动物科学研究所 | Composition for fermenting and deodorizing livestock and poultry breeding excrement and preparation method thereof |
| CN113925023A (en) * | 2021-10-18 | 2022-01-14 | 江苏函夏新农业科技有限公司 | Method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed |
| CN114304075A (en) * | 2022-01-21 | 2022-04-12 | 鲁东大学 | Fruit fly movement device and experiment method |
| CN114586744A (en) * | 2020-12-04 | 2022-06-07 | 中国科学院分子植物科学卓越创新中心 | Method for simultaneously realizing resource treatment of kitchen waste and traditional Chinese medicine dregs through hermetia illucens |
| CN116369285A (en) * | 2023-04-21 | 2023-07-04 | 上海环境卫生工程设计院有限公司 | Method for culturing hermetia illucens by utilizing wet garbage to sort and fix residues |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074007A (en) * | 2019-05-14 | 2019-08-02 | 广州无两生物科技有限公司 | A kind of poultry excrement and urine disposal method |
| CN114586744A (en) * | 2020-12-04 | 2022-06-07 | 中国科学院分子植物科学卓越创新中心 | Method for simultaneously realizing resource treatment of kitchen waste and traditional Chinese medicine dregs through hermetia illucens |
| CN113493288A (en) * | 2021-06-02 | 2021-10-12 | 广东省农业科学院动物科学研究所 | Composition for fermenting and deodorizing livestock and poultry breeding excrement and preparation method thereof |
| CN113925023A (en) * | 2021-10-18 | 2022-01-14 | 江苏函夏新农业科技有限公司 | Method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed |
| CN114304075A (en) * | 2022-01-21 | 2022-04-12 | 鲁东大学 | Fruit fly movement device and experiment method |
| CN114304075B (en) * | 2022-01-21 | 2023-03-31 | 鲁东大学 | Fruit fly movement device and experiment method |
| CN116369285A (en) * | 2023-04-21 | 2023-07-04 | 上海环境卫生工程设计院有限公司 | Method for culturing hermetia illucens by utilizing wet garbage to sort and fix residues |
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