CN106305487A - Biological active padding material of fermentation bed for poultry, preparation method and application - Google Patents
Biological active padding material of fermentation bed for poultry, preparation method and application Download PDFInfo
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- CN106305487A CN106305487A CN201610698285.XA CN201610698285A CN106305487A CN 106305487 A CN106305487 A CN 106305487A CN 201610698285 A CN201610698285 A CN 201610698285A CN 106305487 A CN106305487 A CN 106305487A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 56
- 230000004151 fermentation Effects 0.000 title claims abstract description 56
- 244000144977 poultry Species 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims description 15
- 239000000463 material Substances 0.000 title abstract description 18
- 241000894006 Bacteria Species 0.000 claims abstract description 54
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 36
- 210000003608 fece Anatomy 0.000 claims abstract description 33
- 239000010871 livestock manure Substances 0.000 claims abstract description 19
- 239000002781 deodorant agent Substances 0.000 claims abstract description 7
- 241001446247 uncultured actinomycete Species 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims description 55
- 230000004071 biological effect Effects 0.000 claims description 32
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 31
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 30
- 239000002994 raw material Substances 0.000 claims description 29
- 241000194108 Bacillus licheniformis Species 0.000 claims description 26
- 239000002609 medium Substances 0.000 claims description 25
- 241000193755 Bacillus cereus Species 0.000 claims description 24
- 239000002054 inoculum Substances 0.000 claims description 20
- 241000287828 Gallus gallus Species 0.000 claims description 19
- 239000012531 culture fluid Substances 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 19
- 238000011534 incubation Methods 0.000 claims description 16
- 230000033228 biological regulation Effects 0.000 claims description 15
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- 239000000843 powder Substances 0.000 claims description 15
- 239000011780 sodium chloride Substances 0.000 claims description 15
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
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- 230000004913 activation Effects 0.000 claims description 11
- 229920000742 Cotton Polymers 0.000 claims description 10
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- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 10
- 108010080698 Peptones Proteins 0.000 claims description 10
- 244000061456 Solanum tuberosum Species 0.000 claims description 10
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 10
- 229920002472 Starch Polymers 0.000 claims description 10
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- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 6
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 6
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 239000011790 ferrous sulphate Substances 0.000 claims description 5
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
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- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 4
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- 239000010903 husk Substances 0.000 claims description 4
- MPNNOLHYOHFJKL-UHFFFAOYSA-N peroxyphosphoric acid Chemical compound OOP(O)(O)=O MPNNOLHYOHFJKL-UHFFFAOYSA-N 0.000 claims description 4
- 235000010333 potassium nitrate Nutrition 0.000 claims description 4
- 239000004323 potassium nitrate Substances 0.000 claims description 4
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- 239000011734 sodium Substances 0.000 claims description 4
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K31/00—Housing birds
- A01K31/04—Dropping-boards; Devices for removing excrement
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K31/00—Housing birds
- A01K31/002—Poultry cages, e.g. transport boxes
- A01K31/007—Floors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a biological active padding material of a fermentation bed for poultry. The biological active padding material is prepared by the following materials in percent by weight: 15%-30% of air-permeable material, 20%-50% of water-absorbing material, 2%-20% of auxiliary nutrient material, 5%-20% of other auxiliary materials, 1%-10% of biological deodorant, 10%-30% of poultry manure and 1%-10% of fermenting bacterium agent. The biological active cushioning material disclosed by the invention has the advantages that the biological active padding material can be applied to raising of various poultry, is more reasonable in strain combination, not only has aerobic bacteria, but also has thermophilic actinomycete; simultaneously, by combination of native strains in the soil, the fermentation efficiency is improved by 30%, the bioavailability is increased by not less than 20%. The biological active cushioning material also has the advantages of wider padding material source and relative reduction of cost and the like.
Description
Technical field
The invention belongs to poultry microbial technology field, relate to a kind of complex microorganism activity bedding and padding, further relate to described compound
The preparation method and application of microbial activity bedding and padding.
Background technology
Along with China's aviculture rises year by year to intensive, large-scale development, the sickness rate of poultry disease, poultry manure etc.
Excretion increases year by year, becomes two big major issues of puzzlement China aquaculture.In actual production for poultry manure process only
Be limited to dry, as simple process methods such as agricultural fertilizers, therefore it is very low to the treatment effeciency of poultry feces, leads
Cause a large amount of Excreta to stack under field conditions (factors).Owing to wherein containing the nutritional labelings such as a large amount of protein, under field conditions (factors) can
Big batch fermentation, produces the materials such as ammonia nitrogen, produces odor pollution environment, and additionally a lot of Excretas penetrate into earth's surface, produce soil
A certain degree of pollution, and owing to its energy is too high, cause some crops and vegetable growth bad, it is often more important that feces
Etc. Excreta contains a large amount of pathogenic microorganisms, if can not be inactivated or kill, air, soil, personnel, work may be passed through
The various approach such as tool, vehicle are propagated, and cause breeding environment to deteriorate, and fraction of pathogens microorganism PI people causes serious public affairs
Safe and sanitary problem altogether.The Excretas such as the feces the most effectively processing poultry become a problem anxious to be resolved.It was verified that
The environment such as air, soil, drinking-water can be caused and necessarily stain by physics dry in the sun method, and there is pathophorous probability, and change
Learn sterilization rule and can cause the residual of chemical composition, drinking-water, soil etc. can be caused secondary pollution problem.Bioremediation was both
Pathogenic microorganism can be killed, can effectively convert again nutritional labeling therein, improve phase economic benefit, therefore biological treatment feces etc.
Excreta becomes the first-selection of solution problem.
Fermentation bed is to utilize brand-new natural agriculture theory, a kind of ring proposed in conjunction with Modern microbiological fermentation process technology
Guarantor, safe and effective ecologic breeding method.Its feces mainly discharged by bedding and padding and the poultry of fermentation bed carries out collaborative sending out
Ferment, and then the breeding waste such as rapid conversion excrement, urine, eliminate stench, suppression insect and the procreation of pathogenic bacteria, beneficial microbe simultaneously
Fermenting bed padding and feces of livestock and poultry can be synthesized nutrition such as being available for the edible saccharide of poultry, protein, organic acid, vitamin by flora
Material, provides certain food for poultry, strengthens resistance against diseases simultaneously, promotes the healthy growth of poultry.Along with fermentation bed cultivation
The gradually maturation of technology, applies more and more extensive.The raw material of bedding and padding is mainly sawdust, narrow range at present, and source is few, and cost is relatively
Height, bedding and padding replacement frequency is high, and consumption is big, and maintenance cost is high.Therefore develop wide material sources, cheap fermenting bed padding, enter
One step popularization and application poultry is significant with deep-litter systems.
Summary of the invention
In order to solve, the Excreta treatment effeciencies such as poultry manure are the highest, physics biochemical method processes and there is secondary pollution, disease
Pathogenic microorganism can not effectively inactivate and bedding and padding high in cost of production problem in existing deep-litter systems, the invention provides a kind of poultry and uses
Fermentation bed biological activity bedding and padding, provide the preparation method and application of bedding and padding of the present invention simultaneously.
One poultry of the present invention fermentation bed biological activity bedding and padding, are made up of the raw material of following percentage by weight:
Breathability raw material 15%～30%, water absorption raw material 20%～50%, nutrition accessory 2%～20%, other adjuvants 5%～20%, raw
Thing deodorizer 1%～10%, poultry manure 10%～30% and fermenting agent 1%～10%；
Described breathability raw material is: corn straw, cotton straws, Pericarppium arachidis hypogaeae or rice husk etc.；Wherein corn straw, cotton straws
It is crushed to the segment of 3～4cm；
Described water absorption raw material is: sawdust, wood flour or bark etc.；
Described nutrition accessory is: Testa oryzae, Testa Tritici or distiller grains etc.；
Other described adjuvants are: TONGKANG, cotton seed hulls or corn cob etc.；
Described biological deodorant is: calcium superphosphate or peroxophosphoric acid sodium etc.；
Described poultry manure is: chicken manure or duck excrement etc.；
Described fermenting agent is compound bacteria, by Bacillus cereus, Bacillus licheniformis, yeast and thermophilic actinomycete group
Becoming, fermenting agent, is fully uniformly mixed so as to obtain by above-mentioned each strain after cultivating；Wherein living bacteria count is no less than 109cfu/g；Wax
The hundreds of proportion by subtraction of sample bacillus bacteria is 20%～30%, and the hundreds of proportion by subtraction of Bacillus licheniformis is 20%～30%, and yeast bacterium is hundreds of
Proportion by subtraction is 20%～30%, and the hundreds of proportion by subtraction of Thermophilic Bacteria bacterium is 30%～40%.
Described yeast is: cereuisiae fermentum, Candida utilis etc..
Described a kind of poultry fermentation bed biological activity bedding and padding, raw material weight percentage ratio forms, preferably: breathability raw material
15～25 %, water absorption raw material 30～40%, nutrition accessory 5～8%, other adjuvants 10～15%, biological deodorant 2～5%, poultry
Feces 15～25% and fermenting agent 2～4%.
Microorganism Bacillus cereus, Bacillus licheniformis, yeast and the Thermophilic Bacteria concrete condition used in the present invention
As follows:
Bacillus cereus Classification system Bacillus cereus.Frankland, has the property that and can consume rapidly digestion
Free oxygen in environment in road, forms intestinal low-oxygen environment, promotes that useful anaerobe grows；Energy stimulating animal immune organ
Growth promoter, activated lymphocyte, improves immunoglobulin and antibody horizontal, strengthens cellular immunization and humoral immune function, carry
High herd immunity；Can self synthesis digestibility enzyme, such as protease, amylase, lipase, cellulase etc., at digestive tract
In jointly play a role with endogenous enzymes, improve feed digestibility；
Bacillus licheniformis Classification system Bacillus licheniformis, has the property that and mushrooms out in intestinal
Breeding, causes intestinal low-oxygen environment.Good for health to the bacillus bifidus in intestinal, lactobacillus, bacteroid, peptostreptococcus etc.
The growth and breeding of anaerobe have facilitation, then have antagonism to make the pathogenic bacterium such as staphylococcus, Candida albicans, yeast-like fungus
With, alteration of intestinal flora can be adjusted by this dual function, maintain intestinal microecology balance, thus intestinal tract disease is reached
The purpose treated and prevented.
Yeast Classification system Saccharomyces, has the property that and utilizes aminoacid, saccharide and other Organic substance
Matter, by fermentation, produces the fissional activation material of promotion.Yeast in EM group army for promoting other
The production of the substrate (food) required for effective microbe propagation provides important nutrition assurance.Additionally, the list that yeast produces
Cell protein is the indispensable available nutrient of animal.
Thermophilic actinomycete Classification system Thermophilic actinomycetes, has the property that and is resistant to 70
DEG C high temperature, growth is exceedingly fast, and the metabolism time is short, can ferment under the high temperature conditions, be susceptible to pollute.By fermentation after high temperature, bag
Including pathogenic bacterium at interior most of bacterial death, this bacterium can continue to growth, it is possible to produces Multiple Classes of Antibiotics and various thermally-stabilised
The enzyme of property, such as alpha-amylase, high temperature fiber element enzyme, thermoase and some toolenzymes etc., can directly suppress pathogen.
It obtains the substrate required for harmful mycete and bacterial multiplication in advance, thus suppresses their propagation, and it is useful to create other
The living environment of microbial growth.
The preparation method of above-mentioned fermenting agent, comprises the following steps:
1) bacillus cereus actication of culture, cultivation: culture medium is ordinary nutrient agar culture medium, medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2, inoculum concentration account for culture medium 0.5%～
1%, temperature 20 DEG C～45 DEG C, incubation time 48 hours；Cultivating terminal is that Bacillus cereus bacterium number is not less than the 25% of total bacteria count,
The viable count of culture fluid is 1.0 × 109～1 × 1010cfu/mL；
2) Bacillus licheniformis strain activation, cultivation: culture medium is ordinary nutrient agar culture medium, and medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2, inoculum concentration account for culture medium 0.5%～
1%, temperature 20 DEG C～45 DEG C, incubation time 48 hours；Cultivating terminal is that bacillus licheniformis bacterium number is not less than the 25% of total bacteria count,
The viable count of culture fluid is 1.0 × 109～1 × 1010cfu/mL；
3) yeast seeds activation, cultivation: culture medium is potato dextrose medium, and medium nutrient content is to contain in every 1L
Dehydrated potato powder 6 grams, glucose 20 grams, regulation pH is 5.6 ± 0.2, and inoculum concentration accounts for the 0.5%～1% of culture medium, temperature 25 DEG C～33
DEG C, 48 hours incubation time time；Cultivating terminal is that yeast bacterium number is not less than the 30% of total bacteria count, and the viable count of culture fluid exists
1.0×109～1 × 1010cfu/mL；
4) thermophilic actinomycete actication of culture, cultivation: culture medium is starch agar medium (Gao Shi culture medium): soluble starch 2
Gram, potassium nitrate 0.1 gram, dipotassium hydrogen phosphate 0.05 gram, 0.05 gram of sodium chloride, 0.05 gram of magnesium sulfate, 0.001 gram of ferrous sulfate, water
1000 milliliters, adjustment pH value is to 7.2～7.4, and sterilizing after subpackage, standby, inoculum concentration accounts for the 0.5%～1% of culture medium, temperature 25 DEG C
～37 DEG C, incubation time 48 hours；Cultivating terminal is that yeast bacterium number is not less than the 20% of total bacteria count, and the viable count of culture fluid exists
1.0×109～1 × 1010cfu/mL；
5) it is mixed with fermenting agent: the Bacillus cereus that above steps obtained in proportion, Bacillus licheniformis, yeast
Bacterium and Thermophilic Bacteria culture fully mix, and obtain fermenting agent.
A kind of poultry preparation method of fermentation bed biological activity bedding and padding that the present invention provides, comprises the following steps:
1) breathability raw material is weighed in a ratio, water absorption raw material, nutrition accessory, other adjuvants and biological deodorant, mixed
Close uniformly, then be proportionally added into poultry manure, mix homogeneously.
2) after above-mentioned each raw material mix homogeneously, by prior cultured fermenting agent, in 1～5% ratio mixing, after mixing,
Heap fermentation, surface plastic covering cloth, when bedding and padding middle level temperature reaches 50 DEG C～60 DEG C, be incubated 12～20 hours, ventilate 8～
12 hours, when middle level temperature is down to 20 DEG C～30 DEG C, plastic covering cloth fermentation again, repeats aforesaid operations continuous fermentation 5～7
After it, the bedding and padding of accumulation are raked, obtain biological activity bedding and padding.
The present invention provide the application of fermentation bed biological activity bedding and padding of a kind of poultry, be mainly used in poultry farming, as chicken,
Duck, goose etc.；Its using method is: use layer-stepping and uniform formula association scheme.I.e. adding thickness at the fermentation bed bottom is 10
～the Pericarppium arachidis hypogaeae of 15cm and straw stalk, the poultry of the present invention fermentation that mixed uniformly thickness is 35～30cm is then added above
Bed biological activity bedding and padding, this mode is suitable for poultry and puts down foster or cultivate on the net.
One fermentation bed biological activity bedding and padding of the present invention, preparation method and application have following several respects advantage:
1) strain combination is more reasonable, existing aerobe, has again thermophilic actinomycete, combines again soil original inhabitants' strain simultaneously, fermentation
Efficiency improves 30%.
2) dunnage material source is more extensive, uses in gathering, cost relative reduction.
3) through in-situ fermentation, it is possible to save cleaning feces human and material resources, financial resources, reduce cultivation expense.
4) optimize surrounding air, it is achieved that zero blowdown, improve ecological benefits.
5) these bedding and padding use the time long, can use 3～5 years, only need to add a small amount of strain and adjuvant, than common bedding and padding cost
Lower.
6) bedding and padding can be finally made bio-feritlizer, and after overcuring, killed wherein pathogenic microorganism, decreased sense
Dye animal and the probability of people.
7) after pad material fermentation, macromolecular substances is degraded, and soil more completes with Plant To Nutrient ingredient draws, biological
Utilization rate improves more than 20%.
Detailed description of the invention
Below in conjunction with specific embodiment and comparative example, technical solution of the present invention is described in detail.
Strain name and source that the present invention uses are as follows:
Bacillus cereus Classification system Bacillus cereus.Frankland, this strain is purchased from: Chinese industrial microbial bacteria
Kind of preservation administrative center, Chinese industrial Microbiological Culture Collection administrative center of depositary institution, deposit number be: 21252
Bacillus licheniformis Classification system Bacillus licheniformis, this strain is purchased from: Chinese agriculture microorganism fungus kind
Preservation administrative center, Chinese agriculture Microbiological Culture Collection administrative center of depositary institution, deposit number be: 03298
Thermophilic Actinobacillus Classification system Actinobacillus, this strain is purchased from: the Chinese Academy of Agricultural Sciences's Soil and Fertilizer research
Institute, Chinese agriculture Microbiological Culture Collection administrative center of depositary institution, deposit number be: 11051
Yeast Classification system Candida, this strain is purchased from: China General Microbiological culture presevation administrative center, depositary institution
China General Microbiological culture presevation administrative center, deposit number be: 2.1180
Test one: fermentation bed biological activity bedding and padding, preparation and effect
Embodiment 1
Poultry fermentation bed biological activity bedding and padding, are made up of the raw material of following percentage by weight: corn stalk 24%, sawdust 35%,
Testa oryzae 6%, TONGKANG 10 %, calcium superphosphate 3%, chicken manure 20%, fermenting agent 2%.
Preparing fermenting agent, step is:
1) Bacillus cereus actication of culture, cultivation: culture medium is ordinary nutrient agar culture medium, medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2, and inoculum concentration accounts for the 0.5% of culture medium, temperature
Spend 35 DEG C, incubation time 48 hours；Cultivating terminal is that Bacillus cereus bacterium number is not less than the 25% of total bacteria count, the work of culture fluid
Bacterium number is 1.0 × 109～1 × 1010cfu/mL；
2) Bacillus licheniformis strain activation, cultivation: culture medium is ordinary nutrient agar culture medium, and medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2.Inoculum concentration accounts for the 0.5% of culture medium,
Temperature 35 DEG C, incubation time 48 hours；Cultivating terminal is that bacillus licheniformis bacterium number is not less than the 25% of total bacteria count, culture fluid
Viable count is 1.0 × 109～1 × 1010cfu/mL；
3) yeast seeds activation, cultivation: culture medium is potato dextrose medium, and medium nutrient content is to contain in every 1L
Dehydrated potato powder 6 grams, glucose 20 grams, regulation pH is 5.6 ± 0.2.Inoculum concentration accounts for the 0.5% of culture medium, and temperature 33 DEG C, during cultivation
Between 48 hours；Cultivating terminal is that yeast bacterium number is not less than the 30% of total bacteria count, and the viable count of culture fluid is 1.0 × 109～1 ×
1010cfu/mL；
4) Thermophilic Bacteria actication of culture, cultivation: culture medium is starch agar medium (Gao Shi culture medium): soluble starch 2 grams, nitre
0.1 gram of potassium of acid, dipotassium hydrogen phosphate 0.05 gram, 0.05 gram of sodium chloride, 0.05 gram of magnesium sulfate, 0.001 gram of ferrous sulfate, 2 grams of agar,
1000 milliliters of water, adjustment pH value is to 7.2 ~ 7.4, and sterilizing after subpackage, standby, inoculum concentration accounts for the 0.5% of culture medium, temperature 30 DEG C, training
Support 48 hours time；Cultivating terminal is that yeast bacterium number is not less than the 20% of total bacteria count, and the viable count of culture fluid is 1.0 × 109~
1×1010cfu/mL；
5) culture above steps obtained, by 25% Bacillus cereus, 25% Bacillus licheniformis, 20% yeast
Bacterium and 30% thermophilic Actinobacillus fully mix, and obtain fermenting agent.
Preparation fermentation bed biological activity bedding and padding:
1) 24% corn stalk is weighed by weight, 35% sawdust, 6% Testa oryzae, 10% TONGKANG and 3% calcium superphosphate, mix homogeneously, then
Add 20% chicken manure, mix homogeneously.
2), after above-mentioned each raw material mix homogeneously, 2% fermenting agent, mixing mixing, heap fermentation, surface plastic covering are added
Cloth, when bedding and padding middle level temperature reaches 55 DEG C, keeps 16 hours, ventilates 10 hours, when middle level temperature is down to 25 DEG C, again cover
Lid Polypropylence Sheet ferments, and the bedding and padding of accumulation, after 6 days, are raked, obtained biological activity bedding and padding by repetition aforesaid operations continuous fermentation.
Embodiment 2
Poultry fermentation bed biological activity bedding and padding, are made up of the raw material of following percentage by weight: cotton straws 24%, sawdust 35%,
Testa Tritici 6%, cotton seed hulls 10%, peroxophosphoric acid sodium 3%, chicken manure 20%, fermenting agent 2%.
Preparing fermenting agent, step is:
1) Bacillus cereus actication of culture, cultivation: culture medium is ordinary nutrient agar culture medium, medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2.Inoculum concentration accounts for the 1% of culture medium, temperature
45 DEG C, incubation time 48 hours；Cultivating terminal is that Bacillus cereus bacterium number is not less than the 25% of total bacteria count, the viable bacteria of culture fluid
Number is 1.0 × 109～1 × 1010cfu/mL；
2) Bacillus licheniformis strain activation, cultivation: culture medium is ordinary nutrient agar culture medium, and medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2.Inoculum concentration accounts for the 1% of culture medium, temperature
45 DEG C, incubation time 48 hours；Cultivating terminal is that bacillus licheniformis bacterium number is not less than the 25% of total bacteria count, the viable bacteria of culture fluid
Number is 1.0 × 109～1 × 1010cfu/mL；
3) yeast seeds activation, cultivation, solid culture: culture medium, concrete grammar, parameter；
Culture medium is potato dextrose medium, and medium nutrient content is containing dehydrated potato powder 6 grams, glucose 20 in every 1L
Gram, regulation pH is 5.6 ± 0.2, and inoculum concentration accounts for the 1% of culture medium, temperature 30 DEG C, 48 hours incubation time time；Cultivation terminal is
Yeast bacterium number is not less than the 30% of total bacteria count, and the viable count of culture fluid is 1.0 × 109～1 × 1010cfu/mL；
4) Thermophilic Bacteria actication of culture, cultivation: culture medium is starch agar medium (Gao Shi culture medium): soluble starch 2 grams,
Potassium nitrate 0.1 gram, dipotassium hydrogen phosphate 0.05 gram, 0.05 gram of sodium chloride, 0.05 gram of magnesium sulfate, 0.001 gram of ferrous sulfate, agar 2
Gram, 1000 milliliters of water, adjust pH value to 7.2 ~ 7.4, sterilizing after subpackage, standby, inoculum concentration accounts for the 1% of culture medium, temperature 37 DEG C,
Incubation time 48 hours；Cultivating terminal is that yeast bacterium number is not less than the 20% of total bacteria count, the viable count of culture fluid 1.0 ×
109～1 × 1010cfu/mL；
5) culture above steps obtained, by 20% Bacillus cereus, 20% Bacillus licheniformis, 25% yeast
Fully mix with 35% thermophilic Actinobacillus, obtain fermenting agent.
Preparation fermentation bed biological activity bedding and padding:
1) 24% cotton straws is weighed by weight, 35% sawdust, 6% Testa Tritici, 10% cotton seed hulls and 3% peroxophosphoric acid sodium, mix homogeneously,
Add 20% chicken manure, mix homogeneously.
2), after above-mentioned each raw material mix homogeneously, 2% fermenting agent, mixing mixing, heap fermentation, surface plastic covering are added
Cloth, when bedding and padding middle level temperature reaches 50 DEG C, keeps 20 hours, ventilates 12 hours, when middle level temperature is down to 20 DEG C, again cover
Lid Polypropylence Sheet ferments, and the bedding and padding of accumulation, after 6 days, are raked, obtained biological activity bedding and padding by repetition aforesaid operations continuous fermentation.
Embodiment 3
Poultry fermentation bed biological activity bedding and padding, are made up of the raw material of following percentage by weight: rice husk 24%, wood flour 35%, distiller grains
6%, TONGKANG 10%, calcium superphosphate 3%, chicken manure 20%, fermenting agent 2%.
Preparing fermenting agent, step is:
1) Bacillus cereus actication of culture, cultivation: culture medium is ordinary nutrient agar culture medium, medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2, and inoculum concentration accounts for the 0.8% of culture medium,
Temperature 20 DEG C, incubation time 48 hours；Cultivating terminal is that Bacillus cereus bacterium number is not less than the 25% of total bacteria count, culture fluid
Viable count is 1.0 × 109～1 × 1010cfu/mL；
2) Bacillus licheniformis strain activation, cultivation: culture medium is ordinary nutrient agar culture medium, and medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2.Inoculum concentration accounts for the 0.8% of culture medium,
Temperature 20 DEG C, 48 hours incubation time time；Cultivating terminal is that bacillus licheniformis bacterium number is not less than the 25% of total bacteria count, cultivates
The viable count of liquid is 1.0 × 109～1 × 1010cfu/mL；
3) yeast seeds activation, cultivation: culture medium is potato dextrose medium, and medium nutrient content is to contain in every 1L
Dehydrated potato powder 6 grams, glucose 20 grams, regulation pH is 5.6 ± 0.2, and inoculum concentration accounts for the 0.8% of culture medium, and temperature 25 DEG C, during cultivation
Between 48 hours time；Cultivating terminal is that yeast bacterium number is not less than the 30% of total bacteria count, and the viable count of culture fluid is 1.0 × 109~
1×1010cfu/mL；
4) Thermophilic Bacteria actication of culture, cultivation: culture medium is starch agar medium (Gao Shi culture medium): soluble starch 2 grams,
Potassium nitrate 0.1 gram, dipotassium hydrogen phosphate 0.05 gram, 0.05 gram of sodium chloride, 0.05 gram of magnesium sulfate, 0.001 gram of ferrous sulfate, agar 2
Gram, 1000 milliliters of water, adjust pH value to 7.2 ~ 7.4, sterilizing after subpackage, standby.Inoculum concentration accounts for the 0.8% of culture medium, temperature 25
DEG C, 48 hours incubation time time；Cultivating terminal is that yeast bacterium number is not less than the 20% of total bacteria count, and the viable count of culture fluid exists
1.0×109～1 × 1010cfu/mL；
5) culture above steps obtained, by 20% Bacillus cereus, 20% Bacillus licheniformis, 30% yeast
Bacterium and 40% thermophilic Actinobacillus fully mix, and obtain fermenting agent.
Preparation fermentation bed biological activity bedding and padding:
1) 24% rice husk is weighed by weight, 35% wood flour, 6% distiller grains, 10% TONGKANG, 3% calcium superphosphate, mix homogeneously, add
20% chicken manure, mix homogeneously.
2), after above-mentioned each raw material mix homogeneously, 2% fermenting agent, mixing mixing, heap fermentation, surface plastic covering are added
Cloth, when bedding and padding middle level temperature reaches 60 DEG C, keeps 12 hours, ventilates 8 hours, when middle level temperature is down to 25 DEG C, again cover
Lid Polypropylence Sheet ferments, and the bedding and padding of accumulation, after 6 days, are raked, obtained biological activity bedding and padding by repetition aforesaid operations continuous fermentation.
Comparative example 1 is blank group, is not added with fermenting agent, and comparative example 2～3 is comparative examples 1 preparation method, changes
Fermenting agent component, the effect preferable fermenting agent chosen combination contrasts with embodiment, comparative example 2,3 microbial inoculum total content
It is all 108cfu/g。
Comparative example 2 fermenting agent is: Bacillus cereus+Bacillus licheniformis+yeast；Comparative example 3 fermenting agent is:
Bacillus cereus+Bacillus licheniformis+thermophilic Actinobacillus.
Embodiment 1～3 and comparative example 1～3 bedding and padding treatment effect comparison and analysis:
1) fermenting agent deodorize ability
Table 1 deodorize ability
From table 1, use biological activity bedding and padding of the present invention to be i.e. completely removed stink in 5 days, and comparative example bedding and padding are completely out
At least needing 7 days, efficiency improves nearly 30%, and embodiment group deodorize ability is substantially better than comparative example group.
2) temperature: embodiment 1～3, temperature rises to 60 DEG C of need 36～38 hours, and comparative example 1～3 needs 48～52 little
Time, embodiment effect is substantially better than comparative example, and efficiency improves more than 30%.
Test two: fermentation bed biological activity bedding and padding of the present invention are applied
Fermentation bed activity bedding and padding of the present invention are at the application effect test of certain chicken house
Fermentation bed activity bedding and padding prepared by the embodiment of the present invention 1～3 are proceeded to fermentation bed, is applied to flat chicken farm, ground.Adopt
By layer-stepping and uniform formula association scheme, add Pericarppium arachidis hypogaeae and straw stalk at the fermentation bed bottom, then add above the most mixed
The biological activity bedding and padding of the present invention closed.
Test by the kind of chicken, originate and be grouped:
Kind: AA broiler
Source: plant of COFCO
Packet: close by body weight, the principle of male and female ratio substantially half and half, select with the same batch of kind, be randomly divided into 6 groups.Group 1～
3 is matched group, is respectively adopted the biological pad that comparative example 1 is not added with fermenting agent, prepared by comparative example 2 and 3 fermenting agent and raises；Group
4, the fermentation bed that the fermentation bed biological activity bedding and padding of group 5 and the group 6 respectively embodiment of the present invention 1～3 preparation are made is raised, real
Test effect as follows:
Effect judge index, effect and analysis
1) environment of chicken house affected (see Table 2)
NH in table 2 hen house3And H2S mean concentration (mg/m3)
2) meat chicken production performance affected (see Table 3)
Each group of AA g/ % of meat chicken production performance statistical result tested by table 3
From table 2 and 3, use NH in the environment of chicken house of biological activity bedding and padding of the present invention3And H2S content is all significantly lower than comparison
Group 1～3 raising, each group feedstuff day consumption difference is little, use its feedstuff-meat ratio of broiler that biological activity bedding and padding of the present invention raise and
The meat chicken production performance that survival rate is raised under the conditions of being also significantly better than matched group.
Claims (6)
1. poultry fermentation bed biological activity bedding and padding, it is characterised in that: it is made up of the raw material of following percentage by weight:
Breathability raw material 15%～30%, water absorption raw material 20%～50%, nutrition accessory 2%～20%, other adjuvants 5%～20%, raw
Thing deodorizer 1%～10%, poultry manure 10%～30% and fermenting agent 1%～10%；
Described breathability raw material is: corn straw, cotton straws, Pericarppium arachidis hypogaeae or rice husk etc.；Wherein corn straw, cotton straws
It is crushed to the segment of 3～4cm；
Described water absorption raw material is: sawdust, wood flour or bark；
Described nutrition accessory is: Testa oryzae, Testa Tritici or distiller grains；
Other described adjuvants are: TONGKANG, cotton seed hulls or corn cob；
Described biological deodorant is: calcium superphosphate or peroxophosphoric acid sodium；
Described poultry manure is: chicken manure or duck excrement；
Described fermenting agent is compound bacteria, by Bacillus cereus, Bacillus licheniformis, yeast and thermophilic actinomycete group
Becoming, fermenting agent, is fully uniformly mixed so as to obtain by above-mentioned each strain after cultivating；Wherein living bacteria count is no less than 109cfu/g；Wax
The hundreds of proportion by subtraction of sample bacillus bacteria is 20%～30%, and the hundreds of proportion by subtraction of Bacillus licheniformis is 20%～30%, and yeast bacterium is hundreds of
Proportion by subtraction is 20%～30%, and the hundreds of proportion by subtraction of Thermophilic Bacteria bacterium is 30%～40%.
2. according to the fermentation bed biological activity bedding and padding of a kind of poultry described in claim 1, it is characterised in that: raw material weight hundred
Proportion by subtraction consists of: breathability raw material 15～25%, water absorption raw material 30～40%, nutrition accessory 5～8%, other adjuvants 10～15%,
Biological deodorant 2～5%, poultry manure 15～25% and fermenting agent 2～4%.
3. according to the fermentation bed biological activity bedding and padding of a kind of poultry described in claim 1, it is characterised in that described fermentation
The preparation method of microbial inoculum is:
1) bacillus cereus actication of culture, cultivation: culture medium is ordinary nutrient agar culture medium, medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2, inoculum concentration account for culture medium 0.5%～
1%, temperature 20 DEG C～45 DEG C, incubation time 48 hours；Cultivating terminal is that Bacillus cereus bacterium number is not less than the 25% of total bacteria count,
The viable count of culture fluid is 1.0 × 109～1 × 1010cfu/mL；
2) Bacillus licheniformis strain activation, cultivation: culture medium is ordinary nutrient agar culture medium, and medium nutrient content is every
Containing peptone 20 grams in 1L, beef powder 5 grams, 5 grams of sodium chloride, regulation pH is 7.2 ± 0.2, inoculum concentration account for culture medium 0.5%～
1%, temperature 20 DEG C～45 DEG C, incubation time 48 hours；Cultivating terminal is that bacillus licheniformis bacterium number is not less than the 25% of total bacteria count,
The viable count of culture fluid is 1.0 × 109～1 × 1010cfu/mL；
3) yeast seeds activation, cultivation: culture medium is potato dextrose medium, and medium nutrient content is to contain in every 1L
Dehydrated potato powder 6 grams, glucose 20 grams, regulation pH is 5.6 ± 0.2, and inoculum concentration accounts for the 0.5%～1% of culture medium, temperature 25 DEG C～33
DEG C, incubation time 48 hours；Cultivating terminal is that yeast bacterium number is not less than the 30% of total bacteria count, the viable count of culture fluid 1.0 ×
109～1 × 1010cfu/mL；
4) thermophilic actinomycete actication of culture, cultivation: culture medium is starch agar medium: soluble starch 2 grams, potassium nitrate 0.1
Gram, dipotassium hydrogen phosphate 0.05 gram, 0.05 gram of sodium chloride, 0.05 gram of magnesium sulfate, 0.001 gram of ferrous sulfate, 1000 milliliters of water, adjust
PH value is to 7.2～7.4, and sterilizing after subpackage, standby, inoculum concentration accounts for the 0.5%～1% of culture medium, and temperature 25 DEG C～37 DEG C, during cultivation
Between 48 hours；Cultivating terminal is that yeast bacterium number is not less than the 20% of total bacteria count, and the viable count of culture fluid is 1.0 × 109～1 ×
1010cfu/mL；
5) it is mixed with fermenting agent: the Bacillus cereus that above steps obtained in proportion, Bacillus licheniformis, yeast
Bacterium and Thermophilic Bacteria culture fully mix, and obtain fermenting agent.
4. the preparation method for fermentation bed biological activity bedding and padding of poultry described in any one of claims 1 to 3, including following step
Rapid:
1) breathability raw material is weighed in a ratio, water absorption raw material, nutrition accessory, other adjuvants and biological deodorant, mixed
Close uniformly, then be proportionally added into poultry manure, mix homogeneously；
2) after above-mentioned each raw material mix homogeneously, by prior cultured fermenting agent, in 1～5% ratio mixing, after mixing, pile up
Fermentation, surface plastic covering cloth, when bedding and padding middle level temperature reaches 50 DEG C～60 DEG C, it is incubated 12～20 hours, ventilation 8～12 is little
Time, when middle level temperature is down to 20 DEG C～30 DEG C, plastic covering cloth fermentation again, repetition aforesaid operations continuous fermentation 5～7 days
After, the bedding and padding of accumulation are raked, obtains biological activity bedding and padding.
5. the application for fermentation bed biological activity bedding and padding of poultry described in any one of claims 1 to 3, is mainly used in poultry and supports
Grow.
Application the most according to claim 5, it is characterised in that using method is: use layer-stepping and the uniform formula side of combination
Case, i.e. adds, at the fermentation bed bottom, Pericarppium arachidis hypogaeae and the straw stalk that thickness is 10～15cm, then adds above mixed uniformly
Thickness is the poultry fermentation bed biological activity bedding and padding of 35～30cm, and this mode is suitable for poultry and puts down foster or cultivate on the net.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107593599A (en) * | 2017-10-16 | 2018-01-19 | 安庆市立华牧业有限公司 | The forest land free-ranging method of blue or green pin fiber crops chicken |
| CN107867939A (en) * | 2017-12-01 | 2018-04-03 | 厦门康浩科技有限公司 | A kind of livestock and poultry feces give up outer fermentation bed |
| CN108668917A (en) * | 2018-03-31 | 2018-10-19 | 潍坊友容实业有限公司 | One kind finishing pigs fermenting bed padding of slag containing Suaeda salsa and preparation method thereof |
| CN108684543A (en) * | 2018-03-31 | 2018-10-23 | 潍坊友容实业有限公司 | A kind of salt-soda soil livestock and poultry cultivation fermenting bed padding and preparation method thereof |
| CN110257300A (en) * | 2019-07-16 | 2019-09-20 | 广西富凤农牧集团有限公司 | A kind of chicken manure fermenting agent |
| CN112812990A (en) * | 2020-12-31 | 2021-05-18 | 宜兴艾科森生态环卫设备有限公司 | Compound microbial agent for excrement treatment and application and compounding method thereof |
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| CN101416610A (en) * | 2008-06-24 | 2009-04-29 | 李季 | Pigpen fermentation bed and use thereof |
| CN104686379A (en) * | 2015-02-06 | 2015-06-10 | 陈英旭 | Ecological raising fermentation bed used for live pigs and based on mechanical material turnover as well as manufacturing method and application of ecological raising fermentation bed |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101416610A (en) * | 2008-06-24 | 2009-04-29 | 李季 | Pigpen fermentation bed and use thereof |
| CN104686379A (en) * | 2015-02-06 | 2015-06-10 | 陈英旭 | Ecological raising fermentation bed used for live pigs and based on mechanical material turnover as well as manufacturing method and application of ecological raising fermentation bed |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593599A (en) * | 2017-10-16 | 2018-01-19 | 安庆市立华牧业有限公司 | The forest land free-ranging method of blue or green pin fiber crops chicken |
| CN107867939A (en) * | 2017-12-01 | 2018-04-03 | 厦门康浩科技有限公司 | A kind of livestock and poultry feces give up outer fermentation bed |
| CN108668917A (en) * | 2018-03-31 | 2018-10-19 | 潍坊友容实业有限公司 | One kind finishing pigs fermenting bed padding of slag containing Suaeda salsa and preparation method thereof |
| CN108684543A (en) * | 2018-03-31 | 2018-10-23 | 潍坊友容实业有限公司 | A kind of salt-soda soil livestock and poultry cultivation fermenting bed padding and preparation method thereof |
| CN110257300A (en) * | 2019-07-16 | 2019-09-20 | 广西富凤农牧集团有限公司 | A kind of chicken manure fermenting agent |
| CN112812990A (en) * | 2020-12-31 | 2021-05-18 | 宜兴艾科森生态环卫设备有限公司 | Compound microbial agent for excrement treatment and application and compounding method thereof |
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