CN104293694B - A kind of preparation method of sludge aerobic compost composite bacteria agent - Google Patents
A kind of preparation method of sludge aerobic compost composite bacteria agent Download PDFInfo
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- CN104293694B CN104293694B CN201410444505.7A CN201410444505A CN104293694B CN 104293694 B CN104293694 B CN 104293694B CN 201410444505 A CN201410444505 A CN 201410444505A CN 104293694 B CN104293694 B CN 104293694B
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- 241000894006 Bacteria Species 0.000 title claims abstract description 87
- 239000002361 compost Substances 0.000 title claims abstract description 58
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 46
- 239000002131 composite material Substances 0.000 title claims abstract description 45
- 239000010802 sludge Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 41
- 238000000855 fermentation Methods 0.000 claims abstract description 49
- 230000004151 fermentation Effects 0.000 claims abstract description 49
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 25
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 25
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 24
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 24
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 22
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims abstract description 22
- 238000011081 inoculation Methods 0.000 claims abstract description 20
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 19
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 19
- 238000004321 preservation Methods 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 7
- 239000002609 medium Substances 0.000 claims description 77
- 239000001963 growth medium Substances 0.000 claims description 53
- 239000007787 solid Substances 0.000 claims description 50
- 235000015097 nutrients Nutrition 0.000 claims description 41
- 239000012530 fluid Substances 0.000 claims description 30
- 238000011534 incubation Methods 0.000 claims description 30
- 239000002994 raw material Substances 0.000 claims description 26
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 24
- 230000001954 sterilising effect Effects 0.000 claims description 23
- 239000001888 Peptone Substances 0.000 claims description 22
- 108010080698 Peptones Proteins 0.000 claims description 22
- 239000012153 distilled water Substances 0.000 claims description 22
- 235000019319 peptone Nutrition 0.000 claims description 22
- 238000004659 sterilization and disinfection Methods 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 20
- 229920001817 Agar Polymers 0.000 claims description 19
- 239000008272 agar Substances 0.000 claims description 19
- 239000000284 extract Substances 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 15
- 235000015278 beef Nutrition 0.000 claims description 15
- 239000008103 glucose Substances 0.000 claims description 14
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 12
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 12
- 239000001110 calcium chloride Substances 0.000 claims description 12
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 12
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 12
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 12
- 239000001632 sodium acetate Substances 0.000 claims description 12
- 235000017281 sodium acetate Nutrition 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 11
- 239000012531 culture fluid Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 11
- 239000012138 yeast extract Substances 0.000 claims description 11
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 239000011780 sodium chloride Substances 0.000 claims description 10
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 7
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 7
- 239000011790 ferrous sulphate Substances 0.000 claims description 7
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 7
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 239000004202 carbamide Substances 0.000 claims description 5
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 claims description 5
- 229940099596 manganese sulfate Drugs 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 4
- 239000008101 lactose Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 238000010025 steaming Methods 0.000 claims description 3
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 2
- 241000186660 Lactobacillus Species 0.000 claims description 2
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 2
- 239000001257 hydrogen Substances 0.000 claims description 2
- 229910052739 hydrogen Inorganic materials 0.000 claims description 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims description 2
- 238000009630 liquid culture Methods 0.000 claims description 2
- 241001037822 Bacillus bacterium Species 0.000 claims 2
- 244000025254 Cannabis sativa Species 0.000 claims 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 2
- 239000011574 phosphorus Substances 0.000 claims 2
- 229910052698 phosphorus Inorganic materials 0.000 claims 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims 2
- 229920000053 polysorbate 80 Polymers 0.000 claims 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 241000726221 Gemma Species 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 210000000481 breast Anatomy 0.000 claims 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 210000002268 wool Anatomy 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 12
- 238000009264 composting Methods 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 7
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 6
- 241000222385 Phanerochaete Species 0.000 abstract description 3
- 238000009629 microbiological culture Methods 0.000 abstract description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 6
- 238000012549 training Methods 0.000 description 6
- 238000001816 cooling Methods 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 235000010980 cellulose Nutrition 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000010865 sewage Substances 0.000 description 4
- 208000035985 Body Odor Diseases 0.000 description 3
- 206010040904 Skin odour abnormal Diseases 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 239000010881 fly ash Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- 108010054320 Lignin peroxidase Proteins 0.000 description 1
- 108010059896 Manganese peroxidase Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- -1 excrement Substances 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Zoology (AREA)
- Wood Science & Technology (AREA)
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- Mycology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of preparation method of sludge aerobic compost composite bacteria agent, this method is by bacillus subtilis (the Bacillus subtilis of Chinese industrial Microbiological Culture Collection administrative center (CICC) preservation, numbering 10066), bacillus licheniformis (Bacillus licheniformis, numbering 10103), lactobacillus acidophilus (Lactobacillus acidophilus, numbering 6075), candida utili (Candida utilis, numbering 31272), Phanerochaete chrysosporium (Phanerochaete chrsosporium, numbering 40719), combined inoculation is enlarged culture into fermentation medium and obtains composite bacteria agent in proportion after individually culture.The composite bacteria agent is inoculated in sludge aerobic compost initial stage, heap body is reached time advance 4-6 days of megathermal period, composting cycle shortens 11-14 days, and windrow moisture content declines always nitrogen content raising 15%-20% in 18%-25%, heap body, improves compost effect.
Description
Technical field
The present invention relates to a kind of sludge aerobic compost composite bacteria agent, more particularly to a kind of sludge aerobic compost composite bacteria agent
Preparation method.
Background technology
With the quickening of China's Development of China's Urbanization, municipal sewage treatment amount is improved constantly, sewage disposal technology increasingly into
Ripe, incident problem is a large amount of excess sludges produced when how to handle disposal sewage treatment plant processing sewage.At present
The processing method of disposal of China's excess sludge mainly has anhydration and incineration, sanitary landfills, compost etc..Wherein sludge composting technology is usual
To carry out aerobic compost after being mixed with the auxiliary material such as municipal refuse, feces of livestock and poultry, stalk, wood chip, flyash, be it is a kind of actively effectively
Recycling sludge and harmless treatment disposal options.Traditional sludge composting method is to utilize micro- life indigenous in windrow merely
Thing carries out spontaneous fermentation to complete, because compost indigenous microorganism at initial stage amount is few, breeds slower, tends to cause compost fermentation
Cycle length, generation stink, the low problem of fertilizer efficiency.Therefore, researchers begin to use to sludge composting microbe inoculation function bacterium
Agent come shorten fermentation period, promote compost quick composting and improve composting production quality.
As Chinese Patent Application No. " is carried out to be disclosed in 201010514582.7 specification using compost composite bacteria agent
The method of sludge aerobic compost ", wherein composite bacteria agent are included with composite bacteria agent A and composite bacteria agent B, compound in compost initial vaccination
Microbial inoculum A can promote the fast decoupleds such as protein in windrow, fat, starch, compost temperature is reached the fermentation megathermal period in advance, in height
The warm phase in the form of covering heap body surface seeding a layer thickness as 0.1m composite bacteria B degraded celluloses and hemicellulose usually
Reach compost effect, but it is this using different times with being inoculated with by the way of different, it is necessary to get hold of inoculation opportunity, and
Operation requires strict fine.
Chinese Patent Application No. discloses a kind of " system of compost fermentation complex bacterial agent for 200910113229.5 specification
Preparation Method ", it makes use of the composite bacteria that candida utili, plant lactobacillus, three kinds of strains of bacillus subtilis are constituted, connects
After kind of compost can in reinforcing compost cellulose degraded, lift degrading quality, but compost needs frequent turning, easily meets with into
Heap body odor gas is distributed, and nitrogen loss is serious, and organic matter is lost, and composting cycle is up to 6 weeks, fertilizer efficiency effect ideal not to the utmost etc..
Therefore, the research and development of sludge aerobic compost composite bacteria agent also need to constantly be explored, so that wooden in sludge aerobic compost
Matter cellulose fast degradation, promotes compost quick composting, shortens fermentation period, reduces the loss of nitrogen to reach preferably
Compost treatment effect.
The content of the invention
It is an object of the invention to provide a kind of preparation method of sludge aerobic compost composite bacteria agent, this method is answered
Close microbial inoculum and be applied to sludge aerobic compost, lignocellulosic fast degradation in sludge aerobic compost can be made, promote compost quickly rotten
It is ripe, shorten fermentation period, reduce the loss of nitrogen to reach more preferable compost treatment effect.
The present invention solves above-mentioned technical problem with following technical scheme：
The preparation method of sludge aerobic compost composite bacteria agent of the present invention, in being managed using Chinese industrial Microbiological Culture Collection
The bacillus subtilis (Bacillus subtilis, numbering 10066) of the heart (CICC) preservation, bacillus licheniformis (Bacillus
Licheniformis, numbering 10103), lactobacillus acidophilus (Lactobacillus acidophilus, numbering 6075), production protein
Candida (Candida utilis, numbering 31272), Phanerochaete chrysosporium (Phanerochaete chrsosporium,
Numbering 40719), combined inoculation is enlarged culture into fermentation medium in proportion after individually culture
The preparation method of sludge aerobic compost composite bacteria agent of the present invention, prepare composite bacteria agent when, by bacillus subtilis,
Bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium are in order using bacterium solution volume ratio as 0.2-1：
0.2‐1：0.1‐1：0.2‐1：After 0.1-1.2 mixing, it is seeded in the fermentation medium after high-temperature sterilization, mixed bacteria liquid consumption is
The 4%-12% of fermentation medium weight, quiescent culture 8-12d, cultivation temperature are 30-35 DEG C, when total living microorganism bacterium number reaches
To 1 × 109During more than cuf/ml, the preparation of composite bacteria agent is completed.
The preparation method of sludge aerobic compost composite bacteria agent of the present invention, it is the bacillus subtilis, bacillus licheniformis, thermophilic
Preferred bacterium solution volume ratio is 0.8 in order for Lactobacillus lactis, candida utili, Phanerochaete chrysosporium：0.5：0.5：0.6：
0.2。
The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the fermentation medium is by following weight proportion
Raw material is mixed：Brown sugar 15g-25g, peptone 0.5g-2g, beancake powder 1g-3g, potassium dihydrogen phosphate 0.1g-0.5g, calcium chloride
0.1g-0.5g, ammonium sulfate 0.5g-1.5g, magnesium sulfate 0.1g-0.3g, ferrous sulfate 0.01g-0.02g, sodium acetate 0.3g-1g,
Sodium acid carbonate 0.5g-1.5g, distilled water 1000ml, its pH value are 7.0, and fermentation medium is cold after 121 DEG C of high-temperature sterilization 30min
But just use.
The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the bacillus subtilis bacterium solution is：
Picking Bacillus subtilis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is
35-40 DEG C, incubation time is 18-24h, and then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium
Support, shaking speed is 160r/min, cultivation temperature is 35-40 DEG C, incubation time is 24-48 hours, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes；The bacillus subtilis solid slope culture medium is by the raw material of following weight proportion
It is mixed：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；Institute
Stating fluid nutrient medium is mixed by the raw material of following weight proportion：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride
5g, distilled water 1000ml, pH value 7.0；Solid slope culture medium and fluid nutrient medium are after 121 DEG C of high-temperature sterilization 30min
Cooling is used.
The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the Bacillus licheniformis liquid is：
Picking Bacillus licheniformis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is
25-30 DEG C, incubation time is 18-24h, and then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium
Support, shaking speed is 160r/min, cultivation temperature is 25-30 DEG C, incubation time is 24-48 hours, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes；The solid slope culture medium is mixed by the raw material of following weight proportion：Portugal
Grape sugar 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；The Liquid Culture
Base is mixed by the raw material of following weight proportion：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, distilled water
1000ml, its pH value 7.0；Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min to be made
With.
The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the lactobacillus acidophilus bacterium solution is：
Picking Lactobacillus acidophilus species are inoculated into solid slope culture medium, are placed in incubator and cultivate, cultivation temperature is 35-
40 DEG C, incubation time is 24-36h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium,
Shaking speed is 160r/min, and cultivation temperature is 35-40 DEG C, and incubation time is 48-72 hours, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes；The lactobacillus acidophilus solid slope culture medium is mixed by the raw material of following weight proportion
Conjunction is made：Glucose 10g, lactose 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g,
Tween801.0g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, agar 15g, steaming
Distilled water 1000ml, pH value 5.8-6.8；The fluid nutrient medium is mixed by the raw material of following weight proportion：Glucose
10g, lactose 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g, Tween801.0g, sodium acetate
5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, distilled water 1000ml, its pH value 5.8-6.8；
Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and just used.
The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the candida utili bacterium solution is：
Picking candida utili strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is
25-30 DEG C, incubation time is 16-24h, and then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium
Support, shaking speed is 160r/min, cultivation temperature is 25-30 DEG C, incubation time is 24-48 hours, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes；The candida utili solid slope culture medium is by the raw material of following weight proportion
It is mixed：12brxi. brewer's worts 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g,
Agar 15g, its pH value is nature；The fluid nutrient medium is mixed by the raw material of following weight proportion：12brxi. malt
Juice 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g, its pH value are nature；Solid slope
Culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and used.
The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the Phanerochaete chrysosporium bacterium solution is：
Picking Phanerochaete chrysosporium strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature
For 28-32 DEG C, incubation time is 48-56h, and the inclined-plane mycelium inoculation grown is then carried out into shaking table vibration into fluid nutrient medium
Culture, shaking speed is 80r/min, and cultivation temperature is 28-32 DEG C, and incubation time is 48-72h, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes；The Phanerochaete chrysosporium solid slope culture medium is by the original of following weight proportion
Material is mixed：Potato liquor 1000ml, sucrose 15g, agar 20g, its pH value 5.0-6.0；The fluid nutrient medium be by
The raw material of following weight proportion is mixed：Potato liquor 1000ml, sucrose 15g, its pH value 5.0-6.0；Solid slope is trained
Support base and fluid nutrient medium is cooled down after 121 DEG C of high-temperature sterilization 30min and used.
The above-mentioned yeast extract being related to refers to the extract of standard.
Bacillus subtilis and bacillus licheniformis used in the present invention can promote cellulose fast degradation in compost, simultaneously
Bacillus licheniformis can promote the growth and breeding of lactobacillus acidophilus in composite bacteria agent；Lactobacillus acidophilus can produce small molecular organic acid
The alkaline matters such as the ammonia in neutralization heap body, reduce heap body odor taste；Phanerochaete chrysosporium produces lignin peroxidase, manganese
Peroxidase decomposes so as to accelerate lignin structure.Therefore, in the sludge aerobic compost initial vaccination composite bacteria agent, it can make
Lignocellulosic fast degradation in sludge aerobic compost, promotes compost quick composting, shortens fermentation period, reduces the damage of nitrogen
Lose to reach more preferable compost treatment effect.
Composite bacteria agent prepared by the inventive method is not only limited the use of in sludge aerobic compost, it may also be used for kitchen garbage, livestock and poultry
The compost such as excrement, agricultural wastes.
Embodiment
The inventive method uses the bacillus subtilis of Chinese industrial Microbiological Culture Collection administrative center (CICC) preservation
(Bacillus subtilis, numbering 10066), bacillus licheniformis (Bacillus licheniformis, numbering 10103),
(Candida utilis are compiled for lactobacillus acidophilus (Lactobacillus acidophilus, numbering 6075), candida utili
Number 31272), Phanerochaete chrysosporium (Phanerochaete chrsosporium, numbering 40719), after individually culture
Combined inoculation is enlarged culture into fermentation medium and obtains composite bacteria agent in proportion.
The preparation method of each strain is as follows in the composite bacteria agent：
(1) preparation of bacillus subtilis bacterium solution
Picking Bacillus subtilis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is
35-40 DEG C, incubation time is 18-24h.Then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium
Support, shaking speed is 160r/min, cultivation temperature is 35-40 DEG C, incubation time is 24-48 hours, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes.The bacillus subtilis solid slope culture medium is by the raw material of following weight proportion
It is mixed：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；Institute
State fluid nutrient medium and remove agar for solid slope culture medium formula.Solid slope culture medium and fluid nutrient medium pass through 121 DEG C
Cooling is used after high-temperature sterilization 30min.
(2) preparation of Bacillus licheniformis liquid
Picking Bacillus licheniformis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is
25-30 DEG C, incubation time is 18-24h.Then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium
Support, shaking speed is 160r/min, cultivation temperature is 25-30 DEG C, incubation time is 24-48 hours, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes.The bacillus licheniformis solid slope culture medium is mixed by the raw material of following weight proportion
Conjunction is made：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；It is described
Fluid nutrient medium is that solid slope culture medium formula removes agar.Solid slope culture medium and fluid nutrient medium are by 121 DEG C high
Cool down and use after temperature sterilizing 30min.
(3) preparation of lactobacillus acidophilus bacterium solution
Picking Lactobacillus acidophilus species are inoculated into solid slope culture medium, are placed in incubator and cultivate, cultivation temperature is 35-
40 DEG C, incubation time is 24-36h.Then the inclined-plane colony inoculation grown is subjected to shaking table shaken cultivation into fluid nutrient medium,
Shaking speed is 160r/min, and cultivation temperature is 35-40 DEG C, and incubation time is 48-72 hours, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes.The lactobacillus acidophilus solid slope culture medium is mixed by the raw material of following weight proportion
It is made：Glucose 10g, lactose 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g,
Tween801.0g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, agar 15g, steaming
Distilled water 1000ml, pH value 5.8-6.8；The fluid nutrient medium is that solid slope culture medium formula removes agar.Solid slope culture
Base and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and just used.
(4) preparation of candida utili bacterium solution
Picking candida utili strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is
25-30 DEG C, incubation time is 16-24h.Then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium
Support, shaking speed is 160r/min, cultivation temperature is 25-30 DEG C, incubation time is 24-48 hours, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes.The candida utili solid slope culture medium is mixed by the raw material of following weight proportion
Conjunction is made：12brxi. brewer's worts 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g, fine jade
Fat 15g, pH value is nature；The fluid nutrient medium is that solid slope culture medium formula removes agar.Solid slope culture medium and liquid
Body culture medium is cooled down after 121 DEG C of high-temperature sterilization 30min and used.
(5) preparation of Phanerochaete chrysosporium bacterium solution
Picking Phanerochaete chrysosporium strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature
For 28-32 DEG C, incubation time is 48-56h.Then the slant strains grown are inoculated into progress shaking table vibration in fluid nutrient medium
Culture, shaking speed is 80r/min, and cultivation temperature is 28-32 DEG C, and incubation time is 48-72h, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes.The Phanerochaete chrysosporium solid slope culture medium by following weight proportion raw material
It is mixed：Potato liquor 1000ml, sucrose 15g, agar 20g, pH value 5.0-6.0；The fluid nutrient medium is that solid is oblique
Face culture medium prescription removes agar.Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min to be made
With.
The following is the preparating example of product composite bacteria agent of the present invention：
Embodiment 1:
By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium
Bacterium solution is 0.2 by volume：0.2：0.1：0.2：After 0.1 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is fermentation
The 12% of culture medium weight, in 30 DEG C of quiescent culture 12d, when covering with mycelia in fermentation medium, shakes up microscopic count always micro-
Biological living number can reach 1 × 109Cuf/ml, culture is completed.The fermentative medium formula is：Brown sugar 15g, peptone 1g,
Beancake powder 3g, potassium dihydrogen phosphate 0.1g, calcium chloride 0.1g, ammonium sulfate 0.5g, magnesium sulfate 0.1g, ferrous sulfate 0.01g, sodium acetate
0.3g, sodium acid carbonate 0.5g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium is through 121 DEG C of high-temperature sterilizations
Cooling is just used after 30min.
Embodiment 2:
By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium
Bacterium solution by volume 0.4：0.3：0.1：0.4：After 0.4 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is trained for fermentation
The 10% of base weight is supported, in 35 DEG C of quiescent culture 10d, when covering with mycelia in fermentation medium, the total micro- life of microscopic count is shaken up
Thing live body number can reach 1 × 109Cuf/ml, culture is completed.The formula of the fermentation medium is：Brown sugar 20g, peptone
1.5g, beancake powder 2g, potassium dihydrogen phosphate 0.2g, calcium chloride 0.2g, ammonium sulfate 0.5g, magnesium sulfate 0.2g, ferrous sulfate 0.02g,
Sodium acetate 0.5g, sodium acid carbonate 1g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium goes out through 121 DEG C of high temperature
Cooling is just used after bacterium 30min.
Embodiment 3：
By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium
Bacterium solution by volume 0.8：0.5：0.5：0.6：After 0.2 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is trained for fermentation
The 8% of base weight is supported, in 30 DEG C of quiescent culture 10d, when covering with mycelia in fermentation medium, the total microorganism of microscopic count is shaken up
Live body number can reach 1 × 109Cuf/ml, culture is completed.The formula of the fermentation medium is：Brown sugar 20g, peptone 1.5g,
Beancake powder 2g, potassium dihydrogen phosphate 0.5g, calcium chloride 0.1g, ammonium sulfate 1.5g, magnesium sulfate 0.1g, ferrous sulfate 0.01g, sodium acetate
0.3g, sodium acid carbonate 0.5g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium is through 121 DEG C of high-temperature sterilizations
Cooling is just used after 30min.
This embodiment is optimum embodiment.
Embodiment 4：
By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium
Bacterium solution by volume 0.6：1：1：0.8：After 1.2 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is fermentation medium
The 8% of weight, in 30 DEG C of quiescent culture 8d, when covering with mycelia in fermentation medium, shakes up the total living microorganisms of microscopic count
Number can reach 1 × 109Cuf/ml, culture is completed.The formula of the fermentation medium is：Brown sugar 20g, peptone 1.5, beancake powder
3g, potassium dihydrogen phosphate 0.3g, calcium chloride 0.3g, ammonium sulfate 1.5g, magnesium sulfate 0.2g, ferrous sulfate 0.02g, sodium acetate 0.3g,
Sodium acid carbonate 0.5g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium is after 121 DEG C of high-temperature sterilization 30min
Cooling is just used.
Embodiment 5：
By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium
Bacterium solution by volume 1：0.8：1：1：After 1.2 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is fermentation medium weight
The 12% of amount, in 30 DEG C of quiescent culture 10d, when covering with mycelia in fermentation medium, shakes up the total living microorganisms of microscopic count
Number can reach 1 × 109Cuf/ml, culture is completed.The formula of the fermentation medium is：Brown sugar 25g, peptone 2g, beancake powder
3g, potassium dihydrogen phosphate 0.5g, calcium chloride 0.3g, ammonium sulfate 1.5g, magnesium sulfate 0.3g, ferrous sulfate 0.02g, sodium acetate 1g, carbon
Sour hydrogen sodium 1.5g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium is cold after 121 DEG C of high-temperature sterilization 30min
But just use.
Service condition of the examples detailed above product composite bacteria agent in sludge aerobic compost：
The composite bacteria agent prepared by above-described embodiment 1-5, by 1 in the way of directly spraying when using：100-200 connects
Enter in sludge composting, cool after heap body heats up by fermentation, when end temperature is identical with environment temperature, complete sludge aerobic heap
Fertilizer.Puddled uniformly with auxiliary material before sludge composting, conditioning of mud moisture content is 50%-60%, and the length and width of compost are highly than being 2m:
1m:1m.The auxiliary material is two or more mixture in municipal refuse, feces of livestock and poultry, stalk, wood chip, flyash.Through
The sludge aerobic compost contrast with non-inoculating compound bacterium agent is crossed, heap body reaches the time advance of megathermal period 4-6 days, composting cycle
Shorten 11-14 days, windrow moisture content have dropped 18%-25%, the discharge of heap body odor gas is reduced, and total nitrogen content is improved in windrow
15%-20%, sludge aerobic compost has obtained preferable effect.
Claims (8)
1. a kind of preparation method of sludge aerobic compost composite bacteria agent, it is characterised in that：This method is by bacillus subtilis
Bacillus subtilis, CICC preservations, numbering 10066, bacillus licheniformis Bacillus licheniformis, CICC
Preservation, numbering 10103, lactobacillus acidophilus Lactobacillus acidophilus, CICC preservations, numbering 6075, Candida utilis
Yeast Candida utilis, CICC preservations, numbering 31272, Phanerochaete chrysosporium Phanerochaete
Chrysosporium, CICC preservation, numbering 40719, combined inoculation is entered into fermentation medium in proportion after individually culture
Row expands culture and obtains composite bacteria agent；Prepare composite bacteria agent when, by bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus,
Candida utili, Phanerochaete chrysosporium are in order using bacterium solution volume ratio as 0.2-1：0.2-1：0.1-1：0.2-1：0.1-
After 1.2 mixing, it is seeded in the fermentation medium after high-temperature sterilization, mixed bacteria liquid consumption is the 4%- of fermentation medium weight
12%, quiescent culture 8-12d, cultivation temperature are 30-35 DEG C, when total living microorganism bacterium number reaches 1 × 109More than cuf/ml
When, complete the preparation of composite bacteria agent.
2. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1, it is characterised in that：The withered grass gemma
Bacillus, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium preferred bacterium solution volume ratio in order
For 0.8：0.5：0.5：0.6：0.2.
3. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the fermentation
Culture medium is mixed by the raw material of following weight proportion：Brown sugar 15g-25g, peptone 0.5g-2g, beancake powder 1g-3g, phosphorus
Acid dihydride potassium 0.1g-0.5g, calcium chloride 0.1g-0.5g, ammonium sulfate 0.5g-1.5g, magnesium sulfate 0.1g-0.3g, ferrous sulfate
0.01g-0.02g, sodium acetate 0.3g-1g, sodium acid carbonate 0.5g-1.5g, distilled water 1000ml, its pH value are 7.0, fermented and cultured
Base is cooled down after 121 DEG C of high-temperature sterilization 30min and just used.
4. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the withered grass
The preparation of bacillus bacterium solution is：
Picking Bacillus subtilis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature is 35-40
DEG C, incubation time is 18-24h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, shaken
Bed rotating speed is 160r/min, and cultivation temperature is 35-40 DEG C, and incubation time is 24-48 hours, when clump count reaches 1 × 109cuf/
During ml, prepared by bacterium solution completes；The bacillus subtilis solid slope culture medium is to mix system by the raw material of following weight proportion
Into：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；The liquid
Culture medium is mixed by the raw material of following weight proportion：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, steaming
Distilled water 1000ml, pH value 7.0；Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min
Use.
5. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the lichens
The preparation of bacillus bacterium solution is：
Picking Bacillus licheniformis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature is 25-30
DEG C, incubation time is 18-24h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, shaken
Bed rotating speed is 160r/min, and cultivation temperature is 25-30 DEG C, and incubation time is 24-48 hours, when clump count reaches 1 × 109cuf/
During ml, prepared by bacterium solution completes；The solid slope culture medium is mixed by the raw material of following weight proportion：Glucose 5g,
Beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；The fluid nutrient medium be by with
The raw material of lower weight proportion is mixed：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, distilled water 1000ml, its
PH value 7.0；Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and used.
6. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the acidophilus
The preparation of lactobacillus bacterium solution is：
Picking Lactobacillus acidophilus species are inoculated into solid slope culture medium, are placed in incubator and cultivate, cultivation temperature is 35-40
DEG C, incubation time is 24-36h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, shaken
Bed rotating speed is 160r/min, and cultivation temperature is 35-40 DEG C, and incubation time is 48-72 hours, when clump count reaches 1 × 109cuf/
During ml, prepared by bacterium solution completes；The lactobacillus acidophilus solid slope culture medium is mixed by the raw material of following weight proportion：
Glucose 10g, lactose 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g, Tween80
1.0g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, agar 15g, distilled water
1000ml, pH value 5.8-6.8；The fluid nutrient medium is mixed by the raw material of following weight proportion：Glucose 10g, breast
Sugared 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g, Tween80 1.0g, sodium acetate 5g, phosphorus
Sour hydrogen dipotassium 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, distilled water 1000ml, its pH value 5.8-6.8；Solid is oblique
Face culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and just used.
7. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the production protein
The preparation of Candida bacterium solution is：
Picking candida utili strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature is 25-30
DEG C, incubation time is 16-24h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, shaken
Bed rotating speed is 160r/min, and cultivation temperature is 25-30 DEG C, and incubation time is 24-48 hours, 1 is reached when clump count ×
109During cuf/ml, prepared by bacterium solution completes；The candida utili solid slope culture medium is by the raw material of following weight proportion
It is mixed：12brxi. brewer's worts 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g,
Agar 15g, its pH value is nature；The fluid nutrient medium is mixed by the raw material of following weight proportion：12brxi. malt
Juice 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g, its pH value are nature；Solid slope
Culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and used.
8. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the yellow spore
The preparation of the flat lead fungi bacterium solution of raw wool is：
Picking Phanerochaete chrysosporium strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature is 28-
32 DEG C, incubation time is 48-56h, and the inclined-plane mycelium inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium,
Shaking speed is 80r/min, and cultivation temperature is 28-32 DEG C, and incubation time is 48-72h, when clump count reaches 1 × 109cuf/ml
When, prepared by bacterium solution completes；The Phanerochaete chrysosporium solid slope culture medium is to mix system by the raw material of following weight proportion
Into：Potato liquor 1000ml, sucrose 15g, agar 20g, its pH value 5.0-6.0；The fluid nutrient medium is by following weight
The raw material of proportioning is mixed：Potato liquor 1000ml, sucrose 15g, its pH value 5.0-6.0；Solid slope culture medium and liquid
Body culture medium is cooled down after 121 DEG C of high-temperature sterilization 30min and used.
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