CN104293694B - A kind of preparation method of sludge aerobic compost composite bacteria agent - Google Patents

A kind of preparation method of sludge aerobic compost composite bacteria agent Download PDF

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Publication number
CN104293694B
CN104293694B CN201410444505.7A CN201410444505A CN104293694B CN 104293694 B CN104293694 B CN 104293694B CN 201410444505 A CN201410444505 A CN 201410444505A CN 104293694 B CN104293694 B CN 104293694B
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culture medium
composite bacteria
bacteria agent
fluid nutrient
medium
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CN201410444505.7A
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CN104293694A (en
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周永信
张健
宋海农
徐国涛
朱琦
夏兴良
罗剑云
苏丽梅
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广西博世科环保科技股份有限公司
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a kind of preparation method of sludge aerobic compost composite bacteria agent, this method is by bacillus subtilis (the Bacillus subtilis of Chinese industrial Microbiological Culture Collection administrative center (CICC) preservation, numbering 10066), bacillus licheniformis (Bacillus licheniformis, numbering 10103), lactobacillus acidophilus (Lactobacillus acidophilus, numbering 6075), candida utili (Candida utilis, numbering 31272), Phanerochaete chrysosporium (Phanerochaete chrsosporium, numbering 40719), combined inoculation is enlarged culture into fermentation medium and obtains composite bacteria agent in proportion after individually culture.The composite bacteria agent is inoculated in sludge aerobic compost initial stage, heap body is reached time advance 4-6 days of megathermal period, composting cycle shortens 11-14 days, and windrow moisture content declines always nitrogen content raising 15%-20% in 18%-25%, heap body, improves compost effect.

Description

A kind of preparation method of sludge aerobic compost composite bacteria agent

Technical field

The present invention relates to a kind of sludge aerobic compost composite bacteria agent, more particularly to a kind of sludge aerobic compost composite bacteria agent Preparation method.

Background technology

With the quickening of China's Development of China's Urbanization, municipal sewage treatment amount is improved constantly, sewage disposal technology increasingly into Ripe, incident problem is a large amount of excess sludges produced when how to handle disposal sewage treatment plant processing sewage.At present The processing method of disposal of China's excess sludge mainly has anhydration and incineration, sanitary landfills, compost etc..Wherein sludge composting technology is usual To carry out aerobic compost after being mixed with the auxiliary material such as municipal refuse, feces of livestock and poultry, stalk, wood chip, flyash, be it is a kind of actively effectively Recycling sludge and harmless treatment disposal options.Traditional sludge composting method is to utilize micro- life indigenous in windrow merely Thing carries out spontaneous fermentation to complete, because compost indigenous microorganism at initial stage amount is few, breeds slower, tends to cause compost fermentation Cycle length, generation stink, the low problem of fertilizer efficiency.Therefore, researchers begin to use to sludge composting microbe inoculation function bacterium Agent come shorten fermentation period, promote compost quick composting and improve composting production quality.

As Chinese Patent Application No. " is carried out to be disclosed in 201010514582.7 specification using compost composite bacteria agent The method of sludge aerobic compost ", wherein composite bacteria agent are included with composite bacteria agent A and composite bacteria agent B, compound in compost initial vaccination Microbial inoculum A can promote the fast decoupleds such as protein in windrow, fat, starch, compost temperature is reached the fermentation megathermal period in advance, in height The warm phase in the form of covering heap body surface seeding a layer thickness as 0.1m composite bacteria B degraded celluloses and hemicellulose usually Reach compost effect, but it is this using different times with being inoculated with by the way of different, it is necessary to get hold of inoculation opportunity, and Operation requires strict fine.

Chinese Patent Application No. discloses a kind of " system of compost fermentation complex bacterial agent for 200910113229.5 specification Preparation Method ", it makes use of the composite bacteria that candida utili, plant lactobacillus, three kinds of strains of bacillus subtilis are constituted, connects After kind of compost can in reinforcing compost cellulose degraded, lift degrading quality, but compost needs frequent turning, easily meets with into Heap body odor gas is distributed, and nitrogen loss is serious, and organic matter is lost, and composting cycle is up to 6 weeks, fertilizer efficiency effect ideal not to the utmost etc.. Therefore, the research and development of sludge aerobic compost composite bacteria agent also need to constantly be explored, so that wooden in sludge aerobic compost Matter cellulose fast degradation, promotes compost quick composting, shortens fermentation period, reduces the loss of nitrogen to reach preferably Compost treatment effect.

The content of the invention

It is an object of the invention to provide a kind of preparation method of sludge aerobic compost composite bacteria agent, this method is answered Close microbial inoculum and be applied to sludge aerobic compost, lignocellulosic fast degradation in sludge aerobic compost can be made, promote compost quickly rotten It is ripe, shorten fermentation period, reduce the loss of nitrogen to reach more preferable compost treatment effect.

The present invention solves above-mentioned technical problem with following technical scheme：

The preparation method of sludge aerobic compost composite bacteria agent of the present invention, in being managed using Chinese industrial Microbiological Culture Collection The bacillus subtilis (Bacillus subtilis, numbering 10066) of the heart (CICC) preservation, bacillus licheniformis (Bacillus Licheniformis, numbering 10103), lactobacillus acidophilus (Lactobacillus acidophilus, numbering 6075), production protein Candida (Candida utilis, numbering 31272), Phanerochaete chrysosporium (Phanerochaete chrsosporium, Numbering 40719), combined inoculation is enlarged culture into fermentation medium in proportion after individually culture

The preparation method of sludge aerobic compost composite bacteria agent of the present invention, prepare composite bacteria agent when, by bacillus subtilis, Bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium are in order using bacterium solution volume ratio as 0.2-1： 0.2‐1：0.1‐1：0.2‐1：After 0.1-1.2 mixing, it is seeded in the fermentation medium after high-temperature sterilization, mixed bacteria liquid consumption is The 4%-12% of fermentation medium weight, quiescent culture 8-12d, cultivation temperature are 30-35 DEG C, when total living microorganism bacterium number reaches To 1 × 109During more than cuf/ml, the preparation of composite bacteria agent is completed.

The preparation method of sludge aerobic compost composite bacteria agent of the present invention, it is the bacillus subtilis, bacillus licheniformis, thermophilic Preferred bacterium solution volume ratio is 0.8 in order for Lactobacillus lactis, candida utili, Phanerochaete chrysosporium：0.5：0.5：0.6： 0.2。

The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the fermentation medium is by following weight proportion Raw material is mixed：Brown sugar 15g-25g, peptone 0.5g-2g, beancake powder 1g-3g, potassium dihydrogen phosphate 0.1g-0.5g, calcium chloride 0.1g-0.5g, ammonium sulfate 0.5g-1.5g, magnesium sulfate 0.1g-0.3g, ferrous sulfate 0.01g-0.02g, sodium acetate 0.3g-1g, Sodium acid carbonate 0.5g-1.5g, distilled water 1000ml, its pH value are 7.0, and fermentation medium is cold after 121 DEG C of high-temperature sterilization 30min But just use.

The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the bacillus subtilis bacterium solution is：

Picking Bacillus subtilis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is 35-40 DEG C, incubation time is 18-24h, and then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium Support, shaking speed is 160r/min, cultivation temperature is 35-40 DEG C, incubation time is 24-48 hours, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes；The bacillus subtilis solid slope culture medium is by the raw material of following weight proportion It is mixed：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；Institute Stating fluid nutrient medium is mixed by the raw material of following weight proportion：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, distilled water 1000ml, pH value 7.0；Solid slope culture medium and fluid nutrient medium are after 121 DEG C of high-temperature sterilization 30min Cooling is used.

The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the Bacillus licheniformis liquid is：

Picking Bacillus licheniformis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is 25-30 DEG C, incubation time is 18-24h, and then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium Support, shaking speed is 160r/min, cultivation temperature is 25-30 DEG C, incubation time is 24-48 hours, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes；The solid slope culture medium is mixed by the raw material of following weight proportion：Portugal Grape sugar 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；The Liquid Culture Base is mixed by the raw material of following weight proportion：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, distilled water 1000ml, its pH value 7.0；Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min to be made With.

The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the lactobacillus acidophilus bacterium solution is：

Picking Lactobacillus acidophilus species are inoculated into solid slope culture medium, are placed in incubator and cultivate, cultivation temperature is 35- 40 DEG C, incubation time is 24-36h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, Shaking speed is 160r/min, and cultivation temperature is 35-40 DEG C, and incubation time is 48-72 hours, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes；The lactobacillus acidophilus solid slope culture medium is mixed by the raw material of following weight proportion Conjunction is made：Glucose 10g, lactose 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g, Tween801.0g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, agar 15g, steaming Distilled water 1000ml, pH value 5.8-6.8；The fluid nutrient medium is mixed by the raw material of following weight proportion：Glucose 10g, lactose 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g, Tween801.0g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, distilled water 1000ml, its pH value 5.8-6.8； Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and just used.

The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the candida utili bacterium solution is：

Picking candida utili strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is 25-30 DEG C, incubation time is 16-24h, and then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium Support, shaking speed is 160r/min, cultivation temperature is 25-30 DEG C, incubation time is 24-48 hours, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes；The candida utili solid slope culture medium is by the raw material of following weight proportion It is mixed：12brxi. brewer's worts 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g, Agar 15g, its pH value is nature；The fluid nutrient medium is mixed by the raw material of following weight proportion：12brxi. malt Juice 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g, its pH value are nature；Solid slope Culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and used.

The preparation method of sludge aerobic compost composite bacteria agent of the present invention, the preparation of the Phanerochaete chrysosporium bacterium solution is：

Picking Phanerochaete chrysosporium strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature For 28-32 DEG C, incubation time is 48-56h, and the inclined-plane mycelium inoculation grown is then carried out into shaking table vibration into fluid nutrient medium Culture, shaking speed is 80r/min, and cultivation temperature is 28-32 DEG C, and incubation time is 48-72h, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes；The Phanerochaete chrysosporium solid slope culture medium is by the original of following weight proportion Material is mixed：Potato liquor 1000ml, sucrose 15g, agar 20g, its pH value 5.0-6.0；The fluid nutrient medium be by The raw material of following weight proportion is mixed：Potato liquor 1000ml, sucrose 15g, its pH value 5.0-6.0；Solid slope is trained Support base and fluid nutrient medium is cooled down after 121 DEG C of high-temperature sterilization 30min and used.

The above-mentioned yeast extract being related to refers to the extract of standard.

Bacillus subtilis and bacillus licheniformis used in the present invention can promote cellulose fast degradation in compost, simultaneously Bacillus licheniformis can promote the growth and breeding of lactobacillus acidophilus in composite bacteria agent；Lactobacillus acidophilus can produce small molecular organic acid The alkaline matters such as the ammonia in neutralization heap body, reduce heap body odor taste；Phanerochaete chrysosporium produces lignin peroxidase, manganese Peroxidase decomposes so as to accelerate lignin structure.Therefore, in the sludge aerobic compost initial vaccination composite bacteria agent, it can make Lignocellulosic fast degradation in sludge aerobic compost, promotes compost quick composting, shortens fermentation period, reduces the damage of nitrogen Lose to reach more preferable compost treatment effect.

Composite bacteria agent prepared by the inventive method is not only limited the use of in sludge aerobic compost, it may also be used for kitchen garbage, livestock and poultry The compost such as excrement, agricultural wastes.

Embodiment

The inventive method uses the bacillus subtilis of Chinese industrial Microbiological Culture Collection administrative center (CICC) preservation (Bacillus subtilis, numbering 10066), bacillus licheniformis (Bacillus licheniformis, numbering 10103), (Candida utilis are compiled for lactobacillus acidophilus (Lactobacillus acidophilus, numbering 6075), candida utili Number 31272), Phanerochaete chrysosporium (Phanerochaete chrsosporium, numbering 40719), after individually culture Combined inoculation is enlarged culture into fermentation medium and obtains composite bacteria agent in proportion.

The preparation method of each strain is as follows in the composite bacteria agent：

(1) preparation of bacillus subtilis bacterium solution

Picking Bacillus subtilis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is 35-40 DEG C, incubation time is 18-24h.Then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium Support, shaking speed is 160r/min, cultivation temperature is 35-40 DEG C, incubation time is 24-48 hours, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes.The bacillus subtilis solid slope culture medium is by the raw material of following weight proportion It is mixed：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；Institute State fluid nutrient medium and remove agar for solid slope culture medium formula.Solid slope culture medium and fluid nutrient medium pass through 121 DEG C Cooling is used after high-temperature sterilization 30min.

(2) preparation of Bacillus licheniformis liquid

Picking Bacillus licheniformis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is 25-30 DEG C, incubation time is 18-24h.Then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium Support, shaking speed is 160r/min, cultivation temperature is 25-30 DEG C, incubation time is 24-48 hours, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes.The bacillus licheniformis solid slope culture medium is mixed by the raw material of following weight proportion Conjunction is made：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；It is described Fluid nutrient medium is that solid slope culture medium formula removes agar.Solid slope culture medium and fluid nutrient medium are by 121 DEG C high Cool down and use after temperature sterilizing 30min.

(3) preparation of lactobacillus acidophilus bacterium solution

Picking Lactobacillus acidophilus species are inoculated into solid slope culture medium, are placed in incubator and cultivate, cultivation temperature is 35- 40 DEG C, incubation time is 24-36h.Then the inclined-plane colony inoculation grown is subjected to shaking table shaken cultivation into fluid nutrient medium, Shaking speed is 160r/min, and cultivation temperature is 35-40 DEG C, and incubation time is 48-72 hours, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes.The lactobacillus acidophilus solid slope culture medium is mixed by the raw material of following weight proportion It is made：Glucose 10g, lactose 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g, Tween801.0g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, agar 15g, steaming Distilled water 1000ml, pH value 5.8-6.8；The fluid nutrient medium is that solid slope culture medium formula removes agar.Solid slope culture Base and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and just used.

(4) preparation of candida utili bacterium solution

Picking candida utili strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, and cultivation temperature is 25-30 DEG C, incubation time is 16-24h.Then the inclined-plane colony inoculation grown is carried out to shaking table vibration training into fluid nutrient medium Support, shaking speed is 160r/min, cultivation temperature is 25-30 DEG C, incubation time is 24-48 hours, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes.The candida utili solid slope culture medium is mixed by the raw material of following weight proportion Conjunction is made：12brxi. brewer's worts 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g, fine jade Fat 15g, pH value is nature；The fluid nutrient medium is that solid slope culture medium formula removes agar.Solid slope culture medium and liquid Body culture medium is cooled down after 121 DEG C of high-temperature sterilization 30min and used.

(5) preparation of Phanerochaete chrysosporium bacterium solution

Picking Phanerochaete chrysosporium strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature For 28-32 DEG C, incubation time is 48-56h.Then the slant strains grown are inoculated into progress shaking table vibration in fluid nutrient medium Culture, shaking speed is 80r/min, and cultivation temperature is 28-32 DEG C, and incubation time is 48-72h, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes.The Phanerochaete chrysosporium solid slope culture medium by following weight proportion raw material It is mixed：Potato liquor 1000ml, sucrose 15g, agar 20g, pH value 5.0-6.0；The fluid nutrient medium is that solid is oblique Face culture medium prescription removes agar.Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min to be made With.

The following is the preparating example of product composite bacteria agent of the present invention：

Embodiment 1:

By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium Bacterium solution is 0.2 by volume：0.2：0.1：0.2：After 0.1 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is fermentation The 12% of culture medium weight, in 30 DEG C of quiescent culture 12d, when covering with mycelia in fermentation medium, shakes up microscopic count always micro- Biological living number can reach 1 × 109Cuf/ml, culture is completed.The fermentative medium formula is：Brown sugar 15g, peptone 1g, Beancake powder 3g, potassium dihydrogen phosphate 0.1g, calcium chloride 0.1g, ammonium sulfate 0.5g, magnesium sulfate 0.1g, ferrous sulfate 0.01g, sodium acetate 0.3g, sodium acid carbonate 0.5g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium is through 121 DEG C of high-temperature sterilizations Cooling is just used after 30min.

Embodiment 2:

By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium Bacterium solution by volume 0.4：0.3：0.1：0.4：After 0.4 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is trained for fermentation The 10% of base weight is supported, in 35 DEG C of quiescent culture 10d, when covering with mycelia in fermentation medium, the total micro- life of microscopic count is shaken up Thing live body number can reach 1 × 109Cuf/ml, culture is completed.The formula of the fermentation medium is：Brown sugar 20g, peptone 1.5g, beancake powder 2g, potassium dihydrogen phosphate 0.2g, calcium chloride 0.2g, ammonium sulfate 0.5g, magnesium sulfate 0.2g, ferrous sulfate 0.02g, Sodium acetate 0.5g, sodium acid carbonate 1g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium goes out through 121 DEG C of high temperature Cooling is just used after bacterium 30min.

Embodiment 3：

By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium Bacterium solution by volume 0.8：0.5：0.5：0.6：After 0.2 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is trained for fermentation The 8% of base weight is supported, in 30 DEG C of quiescent culture 10d, when covering with mycelia in fermentation medium, the total microorganism of microscopic count is shaken up Live body number can reach 1 × 109Cuf/ml, culture is completed.The formula of the fermentation medium is：Brown sugar 20g, peptone 1.5g, Beancake powder 2g, potassium dihydrogen phosphate 0.5g, calcium chloride 0.1g, ammonium sulfate 1.5g, magnesium sulfate 0.1g, ferrous sulfate 0.01g, sodium acetate 0.3g, sodium acid carbonate 0.5g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium is through 121 DEG C of high-temperature sterilizations Cooling is just used after 30min.

This embodiment is optimum embodiment.

Embodiment 4：

By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium Bacterium solution by volume 0.6：1：1：0.8：After 1.2 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is fermentation medium The 8% of weight, in 30 DEG C of quiescent culture 8d, when covering with mycelia in fermentation medium, shakes up the total living microorganisms of microscopic count Number can reach 1 × 109Cuf/ml, culture is completed.The formula of the fermentation medium is：Brown sugar 20g, peptone 1.5, beancake powder 3g, potassium dihydrogen phosphate 0.3g, calcium chloride 0.3g, ammonium sulfate 1.5g, magnesium sulfate 0.2g, ferrous sulfate 0.02g, sodium acetate 0.3g, Sodium acid carbonate 0.5g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium is after 121 DEG C of high-temperature sterilization 30min Cooling is just used.

Embodiment 5：

By bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium Bacterium solution by volume 1：0.8：1：1：After 1.2 mixing, it is seeded in fermentation medium, mixed bacteria liquid consumption is fermentation medium weight The 12% of amount, in 30 DEG C of quiescent culture 10d, when covering with mycelia in fermentation medium, shakes up the total living microorganisms of microscopic count Number can reach 1 × 109Cuf/ml, culture is completed.The formula of the fermentation medium is：Brown sugar 25g, peptone 2g, beancake powder 3g, potassium dihydrogen phosphate 0.5g, calcium chloride 0.3g, ammonium sulfate 1.5g, magnesium sulfate 0.3g, ferrous sulfate 0.02g, sodium acetate 1g, carbon Sour hydrogen sodium 1.5g, distilled water 1000ml, fermentation medium pH value 7.0.Fermentation medium is cold after 121 DEG C of high-temperature sterilization 30min But just use.

Service condition of the examples detailed above product composite bacteria agent in sludge aerobic compost：

The composite bacteria agent prepared by above-described embodiment 1-5, by 1 in the way of directly spraying when using：100-200 connects Enter in sludge composting, cool after heap body heats up by fermentation, when end temperature is identical with environment temperature, complete sludge aerobic heap Fertilizer.Puddled uniformly with auxiliary material before sludge composting, conditioning of mud moisture content is 50%-60%, and the length and width of compost are highly than being 2m: 1m:1m.The auxiliary material is two or more mixture in municipal refuse, feces of livestock and poultry, stalk, wood chip, flyash.Through The sludge aerobic compost contrast with non-inoculating compound bacterium agent is crossed, heap body reaches the time advance of megathermal period 4-6 days, composting cycle Shorten 11-14 days, windrow moisture content have dropped 18%-25%, the discharge of heap body odor gas is reduced, and total nitrogen content is improved in windrow 15%-20%, sludge aerobic compost has obtained preferable effect.

Claims (8)

1. a kind of preparation method of sludge aerobic compost composite bacteria agent, it is characterised in that：This method is by bacillus subtilis Bacillus subtilis, CICC preservations, numbering 10066, bacillus licheniformis Bacillus licheniformis, CICC Preservation, numbering 10103, lactobacillus acidophilus Lactobacillus acidophilus, CICC preservations, numbering 6075, Candida utilis Yeast Candida utilis, CICC preservations, numbering 31272, Phanerochaete chrysosporium Phanerochaete Chrysosporium, CICC preservation, numbering 40719, combined inoculation is entered into fermentation medium in proportion after individually culture Row expands culture and obtains composite bacteria agent；Prepare composite bacteria agent when, by bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, Candida utili, Phanerochaete chrysosporium are in order using bacterium solution volume ratio as 0.2-1：0.2-1：0.1-1：0.2-1：0.1- After 1.2 mixing, it is seeded in the fermentation medium after high-temperature sterilization, mixed bacteria liquid consumption is the 4%- of fermentation medium weight 12%, quiescent culture 8-12d, cultivation temperature are 30-35 DEG C, when total living microorganism bacterium number reaches 1 × 109More than cuf/ml When, complete the preparation of composite bacteria agent.
2. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1, it is characterised in that：The withered grass gemma Bacillus, bacillus licheniformis, lactobacillus acidophilus, candida utili, Phanerochaete chrysosporium preferred bacterium solution volume ratio in order For 0.8：0.5：0.5：0.6：0.2.
3. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the fermentation Culture medium is mixed by the raw material of following weight proportion：Brown sugar 15g-25g, peptone 0.5g-2g, beancake powder 1g-3g, phosphorus Acid dihydride potassium 0.1g-0.5g, calcium chloride 0.1g-0.5g, ammonium sulfate 0.5g-1.5g, magnesium sulfate 0.1g-0.3g, ferrous sulfate 0.01g-0.02g, sodium acetate 0.3g-1g, sodium acid carbonate 0.5g-1.5g, distilled water 1000ml, its pH value are 7.0, fermented and cultured Base is cooled down after 121 DEG C of high-temperature sterilization 30min and just used.
4. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the withered grass The preparation of bacillus bacterium solution is：
Picking Bacillus subtilis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature is 35-40 DEG C, incubation time is 18-24h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, shaken Bed rotating speed is 160r/min, and cultivation temperature is 35-40 DEG C, and incubation time is 24-48 hours, when clump count reaches 1 × 109cuf/ During ml, prepared by bacterium solution completes；The bacillus subtilis solid slope culture medium is to mix system by the raw material of following weight proportion Into：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；The liquid Culture medium is mixed by the raw material of following weight proportion：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, steaming Distilled water 1000ml, pH value 7.0；Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min Use.
5. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the lichens The preparation of bacillus bacterium solution is：
Picking Bacillus licheniformis strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature is 25-30 DEG C, incubation time is 18-24h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, shaken Bed rotating speed is 160r/min, and cultivation temperature is 25-30 DEG C, and incubation time is 24-48 hours, when clump count reaches 1 × 109cuf/ During ml, prepared by bacterium solution completes；The solid slope culture medium is mixed by the raw material of following weight proportion：Glucose 5g, Beef extract 1g, peptone 10g, sodium chloride 5g, agar 15g, distilled water 1000ml, pH value 7.0；The fluid nutrient medium be by with The raw material of lower weight proportion is mixed：Glucose 5g, beef extract 1g, peptone 10g, sodium chloride 5g, distilled water 1000ml, its PH value 7.0；Solid slope culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and used.
6. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the acidophilus The preparation of lactobacillus bacterium solution is：
Picking Lactobacillus acidophilus species are inoculated into solid slope culture medium, are placed in incubator and cultivate, cultivation temperature is 35-40 DEG C, incubation time is 24-36h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, shaken Bed rotating speed is 160r/min, and cultivation temperature is 35-40 DEG C, and incubation time is 48-72 hours, when clump count reaches 1 × 109cuf/ During ml, prepared by bacterium solution completes；The lactobacillus acidophilus solid slope culture medium is mixed by the raw material of following weight proportion： Glucose 10g, lactose 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g, Tween80 1.0g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, agar 15g, distilled water 1000ml, pH value 5.8-6.8；The fluid nutrient medium is mixed by the raw material of following weight proportion：Glucose 10g, breast Sugared 5g, beef extract 5g, yeast extract 10g, peptone 10g, diammonium hydrogen citrate 2g, Tween80 1.0g, sodium acetate 5g, phosphorus Sour hydrogen dipotassium 2g, magnesium sulfate 0.1g, manganese sulfate 0.05g, calcium carbonate 10g, distilled water 1000ml, its pH value 5.8-6.8；Solid is oblique Face culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and just used.
7. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the production protein The preparation of Candida bacterium solution is：
Picking candida utili strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature is 25-30 DEG C, incubation time is 16-24h, and the inclined-plane colony inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, shaken Bed rotating speed is 160r/min, and cultivation temperature is 25-30 DEG C, and incubation time is 24-48 hours, 1 is reached when clump count × 109During cuf/ml, prepared by bacterium solution completes；The candida utili solid slope culture medium is by the raw material of following weight proportion It is mixed：12brxi. brewer's worts 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g, Agar 15g, its pH value is nature；The fluid nutrient medium is mixed by the raw material of following weight proportion：12brxi. malt Juice 1000ml, yeast extract 5g, urea 0.2g, dipotassium hydrogen phosphate 2g, calcium chloride 0.02g, its pH value are nature；Solid slope Culture medium and fluid nutrient medium are cooled down after 121 DEG C of high-temperature sterilization 30min and used.
8. the preparation method of sludge aerobic compost composite bacteria agent according to claim 1 or claim 2, it is characterised in that the yellow spore The preparation of the flat lead fungi bacterium solution of raw wool is：
Picking Phanerochaete chrysosporium strain is inoculated into solid slope culture medium, is placed in incubator and cultivates, cultivation temperature is 28- 32 DEG C, incubation time is 48-56h, and the inclined-plane mycelium inoculation grown is then carried out into shaking table shaken cultivation into fluid nutrient medium, Shaking speed is 80r/min, and cultivation temperature is 28-32 DEG C, and incubation time is 48-72h, when clump count reaches 1 × 109cuf/ml When, prepared by bacterium solution completes；The Phanerochaete chrysosporium solid slope culture medium is to mix system by the raw material of following weight proportion Into：Potato liquor 1000ml, sucrose 15g, agar 20g, its pH value 5.0-6.0；The fluid nutrient medium is by following weight The raw material of proportioning is mixed：Potato liquor 1000ml, sucrose 15g, its pH value 5.0-6.0；Solid slope culture medium and liquid Body culture medium is cooled down after 121 DEG C of high-temperature sterilization 30min and used.
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