CN109385382B - Preparation method and application of composite microbial inoculum for sludge composting - Google Patents
Preparation method and application of composite microbial inoculum for sludge composting Download PDFInfo
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- CN109385382B CN109385382B CN201811423120.7A CN201811423120A CN109385382B CN 109385382 B CN109385382 B CN 109385382B CN 201811423120 A CN201811423120 A CN 201811423120A CN 109385382 B CN109385382 B CN 109385382B
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 26
- 239000002131 composite material Substances 0.000 title claims abstract description 22
- 239000002361 compost Substances 0.000 claims abstract description 32
- 238000000855 fermentation Methods 0.000 claims abstract description 24
- 230000004151 fermentation Effects 0.000 claims abstract description 24
- 239000001963 growth medium Substances 0.000 claims description 51
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 42
- 238000002156 mixing Methods 0.000 claims description 37
- 230000001580 bacterial effect Effects 0.000 claims description 35
- 239000007788 liquid Substances 0.000 claims description 35
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 239000002994 raw material Substances 0.000 claims description 26
- 235000015278 beef Nutrition 0.000 claims description 25
- 239000001888 Peptone Substances 0.000 claims description 22
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- 235000019319 peptone Nutrition 0.000 claims description 22
- 239000011780 sodium chloride Substances 0.000 claims description 21
- 238000012258 culturing Methods 0.000 claims description 19
- 230000001954 sterilising effect Effects 0.000 claims description 19
- 238000001816 cooling Methods 0.000 claims description 18
- 241000194108 Bacillus licheniformis Species 0.000 claims description 16
- 244000063299 Bacillus subtilis Species 0.000 claims description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 15
- 241000193385 Geobacillus stearothermophilus Species 0.000 claims description 14
- 239000007836 KH2PO4 Substances 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 14
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 10
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 10
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- 229930003231 vitamin Natural products 0.000 claims description 9
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- 229940088594 vitamin Drugs 0.000 claims description 9
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 9
- 241000187747 Streptomyces Species 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 241001150381 Bacillus altitudinis Species 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 6
- 239000010871 livestock manure Substances 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 4
- 244000144972 livestock Species 0.000 claims description 4
- 244000144977 poultry Species 0.000 claims description 4
- 239000010902 straw Substances 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims description 3
- 239000010813 municipal solid waste Substances 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 241000626621 Geobacillus Species 0.000 claims description 2
- 241000187398 Streptomyces lividans Species 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 2
- 241000187175 Streptomyces violaceus Species 0.000 claims 2
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- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 description 22
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 20
- 239000008103 glucose Substances 0.000 description 20
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- 239000000047 product Substances 0.000 description 5
- 241000187095 Streptomyces purpureus Species 0.000 description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000001502 supplementing effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
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- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 241000946829 Streptomyces mutabilis Species 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
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- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
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- 229910052757 nitrogen Inorganic materials 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a preparation method and application of a composite microbial inoculum for sludge composting. The composite microbial inoculum is mainly applied to high-temperature sludge composting, has quick fermentation starting time and long high-temperature maintaining time, can shorten the composting time, reduces the moisture content of the compost, has low production cost, is environment-friendly and pollution-free, has excellent and high-efficiency performance, and has better economic benefit and social benefit.
Description
Technical Field
The invention belongs to the technical field of microbial fermentation, and particularly relates to a preparation method and application of a composite microbial inoculum for sludge composting.
Background
Along with the continuous acceleration of the urbanization level in China, the treatment capacity of urban sewage is continuously improved, and the treatment of a large amount of excess sludge generated during sewage treatment becomes a main problem of urban development. At present, the method adopted by China for treating sludge mainly comprises sludge dewatering, drying incineration, sanitary landfill, composting and the like, wherein the sludge composting technology is usually to mix the sludge with auxiliary materials such as straws, sawdust, fly ash, livestock manure and the like and then add a microbial agent for aerobic composting, and the method is an efficient, green and harmless sludge recycling treatment mode. The traditional sludge composting method mainly utilizes indigenous microorganisms in the compost to carry out natural fermentation, but the problems of long fermentation period, low fertilizer efficiency, peculiar smell generation and the like of the compost are often caused due to less indigenous microorganisms in the initial stage and slower propagation. Therefore, researchers have begun to use complex microbial agents inoculated into compost to shorten the fermentation period, promote the rapid decomposition of compost and improve the quality of compost products.
In recent years, microbial agents have been applied to sludge composting, but many problems still exist, such as single effect of some microorganisms, complex operation, unsatisfactory fertilizer effect and the like, so that the microbial agents are difficult to be widely applied. Therefore, research and development of the sludge compost composite microbial inoculum are required to be continuously explored and developed, so that lignocellulose in the sludge compost is rapidly degraded, the compost is rapidly decomposed, the fermentation period is shortened, and the fertility loss is reduced, so that a better compost treatment effect is achieved.
Disclosure of Invention
The invention aims to provide a preparation method and application of a composite microbial inoculum for sludge compost.
In order to achieve the aim, the invention adopts the following technical scheme:
the invention discloses a preparation method of a composite microbial inoculum for sludge compost, which comprises the steps of separately culturing Bacillus altitudinis (CICC preservation, preservation number 24238, preservation date 2017 month and 12 days), purple Streptomyces mutabilis (CICC preservation, preservation number 23630, preservation date 2008 month 11 and 21 days), Geobacillus thermophilus (ACCC preservation, preservation number 10253, preservation date 2009 month 13), Bacillus subtilis (ACCC), ACCC preservation, preservation number 19742, preservation date 2012 month 9 and 8 days) and Bacillus (Bacillus licheniformis, ACCC preservation, preservation number 04312, preservation date 7 month and 24 days) preserved by China industrial and agricultural microbial strain preservation management center (CICC and ACCC), mixing according to volume ratio, inoculating the obtained mixed bacterial liquid into mixed culture medium, fermenting and culturing the mixed bacterial liquid, obtaining the composite microbial inoculum for sludge composting.
When in mixing, the volume ratio of the bacteria liquid of the geobacillus altitudinis, the streptomyces lividans, the geobacillus stearothermophilus, the bacillus subtilis and the bacillus licheniformis is 0.6-1.2: 0.6-1.2: 0.3-0.8: 0.6-1.2: 0.3-0.8.
The preferred volume ratio is: 0.8: 0.8: 0.5: 0.8: 0.5.
the fermentation medium is prepared by mixing the following raw materials in parts by weight: 15-25g of brown sugar, 5-10g of peptone, 3-5g of beef extract, 2-5g of NaCl and KH2PO4 0.75-1.5g，MgSO4.7H2O 0.5-1g，K2HPO40.8-1g, 110-15 mg vitamin B, and 1L potato extract (200 g peeled potato is taken, cut into small pieces, 1L water is added, boiled for 30min, potato pieces are filtered, the filtrate is made up to 1L), the pH is 7, the mixture is sterilized at 121 ℃ for 30min, and then the mixture is cooled for use.
Inoculating the mixed bacteria liquid toThe inoculation volume ratio in the fermentation medium is 5-15%, the culture temperature is 30-37 ℃, the culture time is 3-5d, the stirring speed is 160-200rpm, the ventilation volume is 1:1.5-2, the stirring interval time is 2h, the stirring is carried out for 2min, and when the viable count reaches 1 × 108And when the CFU/mL is higher than the set value, the preparation of the complex microbial inoculum is completed.
The preparation process of the highland bacillus bacteria liquid comprises the following steps: selecting Bacillus altitudinis strain, inoculating to solid slant culture, culturing in culture medium at 37 deg.C for 24 hr, inoculating to liquid culture medium after the slant culture medium grows out, shaking table shaking culture at rotation speed of 150-8The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 5g of peptone, 3g of beef extract, 5g of NaCl and 15g of agar, supplementing distilled water to 1L, adjusting the pH value to 7, sterilizing at 121 ℃ for 30min, and cooling for use; the liquid culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 5g of peptone, 3g of beef extract and 5g of NaCl, adding distilled water to 1L, adjusting the pH value to 7, sterilizing at 121 ℃ for 30min, and cooling for use.
The preparation process of the purple streptomyces mutans bacterial liquid comprises the following steps: selecting purple streptomyces mutans strain, inoculating into solid slant culture, culturing in culture medium at 37 deg.C for 24 hr, inoculating into liquid culture medium after the slant culture medium grows out, shaking table shaking culturing at rotation speed of 150-8The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared by mixing the following raw materials in parts by weight: glucose 20g, KH2PO4 3g，MgSO4.7H21.5g of O, 120 g of vitamin B120 mg, 15g of agar, 1L of potato extract (200 g of peeled potatoes are taken and cut into small pieces, 1L of water is added to the small pieces and boiled for 30min, the potato pieces are filtered out, the filtrate is made up to 1L), the pH value is 6, the mixture is sterilized at 121 ℃ for 30min and then is cooled for use; the liquid culture medium is prepared from the following components in parts by weightThe raw materials are mixed to prepare: glucose 20g, KH2PO4 3g，MgSO4.7H2O1.5g, vitamin B120 mg, and 1L of potato extract (200 g of peeled potato is taken, cut into small pieces, 1L of water is added, boiled for 30min, the potato pieces are filtered off, the filtrate is made up to 1L), the pH is 6, the potato extract is sterilized at 121 ℃ for 30min, and then the potato extract is cooled for use.
The preparation process of the geobacillus stearothermophilus bacterial liquid comprises the following steps: selecting Geobacillus stearothermophilus strain, inoculating into solid slant culture medium, culturing at 37 deg.C for 48 hr, inoculating into liquid culture medium after slant culture medium grows out, shaking table at rotation speed of 150-8The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 9g of peptone, 5g of beef extract, 2g of NaCl and K2HPO4 1g，KH2PO40.75g of agar and 15g of agar, adding distilled water to 1L, sterilizing at 121 ℃ for 30min, and cooling; the liquid culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 9g of peptone, 5g of beef extract, 5g of NaCl and K2HPO4 1g，KH2PO40.75g, distilled water was added to 1L, pH 7, sterilized at 121 ℃ for 30min, and then cooled for use.
The preparation process of the bacillus subtilis liquid comprises the following steps: selecting Bacillus subtilis strain, inoculating to solid slant culture, culturing in culture medium at 37 deg.C for 24 hr, inoculating to liquid culture medium after the slant culture medium grows out, shaking table shaking culturing at rotation speed of 150-8The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared by mixing the following raw materials in parts by weight: 5.0g of glucose, 10g of peptone, 1g of beef extract, 5g of NaCl and 15g of agar, supplementing distilled water to 1L, adjusting the pH value to 7, sterilizing at 121 ℃ for 30min, and cooling for use; the liquid isThe culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 10g of peptone, 1g of beef extract and 5g of NaCl, adding distilled water to 1L, adjusting the pH value to 7, sterilizing at 121 ℃ for 30min, and cooling for use.
The preparation process of the bacillus licheniformis liquid comprises the following steps: selecting Bacillus licheniformis strain, inoculating to solid slant culture, culturing in culture medium at 37 deg.C for 24 hr, inoculating to liquid culture medium after the slant culture medium grows out, shaking table shaking culturing at rotation speed of 150-8The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared by mixing the following raw materials in parts by weight: 5.0g of glucose, 10g of peptone, 1g of beef extract, 5g of NaCl and 15g of agar, supplementing distilled water to 1L, adjusting the pH value to 7, sterilizing at 121 ℃ for 30min, and cooling for use; the liquid culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 10g of peptone, 1g of beef extract and 5g of NaCl, adding distilled water to 1L, adjusting the pH value to 7, sterilizing at 121 ℃ for 30min, and cooling for use.
The application method of the composite microbial inoculum prepared by the invention comprises the following steps:
according to the following steps: the mass ratio of 500-600 is that the composite microbial inoculum is directly sprayed into the compost, and when the compost is fermented, heated and then cooled, and finally the temperature is the same as the ambient temperature, the sludge composting is completed. Before composting, the compost and auxiliary materials are mixed and stirred uniformly, the water content is adjusted to be 50% -60%, and the auxiliary materials are mixtures of two or more than two of straws, sawdust, livestock and poultry manure, rice hulls and municipal garbage.
Compared with the prior art, the invention has the beneficial effects that: the invention is mainly applied to high-temperature sludge composting, has quick fermentation starting temperature and long high-temperature maintaining time, can shorten the composting time, reduces the water content of the compost, has low production cost, is environment-friendly and pollution-free, has excellent and high-efficiency performance, and has better economic benefit and social benefit. The details are as follows:
1. the selected highland bacillus, bacillus subtilis and bacillus licheniformis can all generate enzymes such as protease, amylase, bacteriocin and the like to promote the rapid degradation of lignocellulose in the compost, the fermentation product can promote the growth of root systems, and meanwhile, the bacillus licheniformis can promote the growth and the propagation of purple streptomyces mutans in the composite microbial inoculum; the purple streptomyces mutans and the geobacillus stearothermophilus have the functions of dissolving phosphorus and potassium and activating silicon, calcium and magnesium elements in sludge, can improve the stress resistance of crops, can secrete various enzymes and plant growth hormone, and can release potassium and phosphorus after thalli die and can be absorbed by the plants.
2. The selected microorganisms are composite microbial inoculum, have strong combination and synergistic capability, are not mutually exclusive, are mutually coordinated in compost, and have fast fermentation temperature rise and long high-temperature maintenance time. The product can be directly put in without complex process, and is convenient for use.
3. The composite microbial inoculum prepared by the invention is not only used for sludge composting, but also can be used for composting such as livestock and poultry manure, agricultural wastes, kitchen wastes and the like.
Detailed Description
The invention uses Bacillus altitudinis (CICC preservation, No. 24238), purple Streptomyces virobasibilis (CICC preservation, No. 23630), Geobacillus thermophilus (ACCC preservation, No. 10253), Bacillus subtilis (ACCC preservation, No. 19742) and Bacillus licheniformis (ACCC preservation, No. 04312) preserved by China industrial and agricultural microbial strain preservation management center (CICC and ACCC), and the composite bacterial agent for sludge composting is obtained by mixing and inoculating to fermentation medium according to volume ratio after being cultured alone and then carrying out amplification culture.
The preparation method of each strain in the composite microbial inoculum comprises the following steps:
(1) the preparation method of the highland bacillus bacterial liquid comprises the following steps: selecting Bacillus altitudinis strain, inoculating to solid slant culture medium, culturing at 37 deg.C for 24 hr, inoculating to liquid culture medium, and shaking at 150Culturing at 35-37 deg.C for 36-48h at-180 r/min until viable count reaches 1 × 108The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 5g of peptone, 3g of beef extract, 5g of NaCl, 15g of agar and 1L of supplemented distilled water, wherein the pH value is 7, and the beef extract is sterilized at 121 ℃ for 30min and then cooled for use; the liquid culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 5g of peptone, 3g of beef extract and 5g of NaCl, adding distilled water to 1L, adjusting the pH to 7, sterilizing at 121 ℃ for 30min, and cooling for use.
(2) The preparation method of the purple streptomyces mutans bacterial liquid comprises the following steps: selecting purple streptomyces mutans strain, inoculating into solid slant culture, culturing in culture medium at 37 deg.C for 24 hr, inoculating into liquid culture medium after the slant culture medium grows out, shaking table shaking culturing at rotation speed of 150-8The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared by mixing the following raw materials in parts by weight: glucose 20g, KH2PO4 3g、MgSO4.7H2O1.5g, vitamin B120 mg, agar 15g, and potato extractive solution 1L (peeling potato 200g, cutting into small pieces, adding water 1L, boiling for 30min, filtering to remove potato pieces, adding filtrate to 1L), adjusting pH to 6, sterilizing at 121 deg.C for 30min, and cooling; the liquid culture medium is prepared by mixing the following raw materials in parts by weight: glucose 20g, KH2PO4 3g、MgSO4.7H2O1.5g, vitamin B120 mg, and 1L potato extractive solution (peeling potato 200g, cutting into small pieces, adding water 1L, boiling for 30min, filtering to remove potato pieces, adding filtrate to 1L), adjusting pH to 6, sterilizing at 121 deg.C for 30min, and cooling.
(3) The preparation method of the geobacillus stearothermophilus bacterial liquid comprises the following steps: selecting Geobacillus stearothermophilus strain, inoculating into solid slant culture medium, culturing at 37 deg.C for 48 hr, inoculating into liquid culture medium after slant culture medium grows out, shaking, and culturingThe bed rotation speed is 150-8The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 9g of peptone, 5g of beef extract, NaCl2g and K2HPO4 1g、KH2PO40.75g of agar and 15g of agar, adding distilled water to 1L, sterilizing at 121 ℃ for 30min, and cooling; the liquid culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 9g of peptone, 5g of beef extract, 5g of NaCl and K2HPO4 1g、KH2PO40.75g, distilled water was added to 1L, pH 7, sterilized at 121 ℃ for 30min, and then cooled for use.
(4) The preparation method of the bacillus subtilis liquid comprises the following steps: selecting Bacillus subtilis strain, inoculating to solid slant culture, culturing in culture medium at 37 deg.C for 24 hr, inoculating to liquid culture medium after the slant culture medium grows out, shaking table shaking culturing at rotation speed of 150-8The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared by mixing the following raw materials in parts by weight: 5.0g of glucose, 10g of peptone, 1g of beef extract, 5g of NaCl, 15g of agar and 1L of supplemented distilled water, wherein the pH value is 7, and the beef extract is sterilized at 121 ℃ for 30min and then cooled for use; the liquid culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 10g of peptone, 1g of beef extract and 5g of NaCl, adding distilled water to 1L, adjusting the pH to 7, sterilizing at 121 ℃ for 30min, and cooling for use.
(5) The preparation method of the bacillus licheniformis liquid comprises the following steps: selecting Bacillus licheniformis strain, inoculating to solid slant culture, culturing in culture medium at 37 deg.C for 24 hr, inoculating to liquid culture medium after the slant culture medium grows out, shaking table shaking culturing at rotation speed of 150-8The preparation of the bacterial liquid is finished when the bacterial concentration is CFU/mL; the solid slant culture medium is prepared from the following componentsThe raw materials with the weight ratio are mixed to prepare: 5.0g of glucose, 10g of peptone, 1g of beef extract, 5g of NaCl, 15g of agar and 1L of supplemented distilled water, wherein the pH value is 7, and the beef extract is sterilized at 121 ℃ for 30min and then cooled for use; the liquid culture medium is prepared by mixing the following raw materials in parts by weight: 5g of glucose, 10g of peptone, 1g of beef extract and 5g of NaCl, adding distilled water to 1L, adjusting the pH to 7, sterilizing at 121 ℃ for 30min, and cooling for use.
The following is a preparation example of the complex microbial inoculum of the invention product:
example 1:
the method comprises the following steps of (1) mixing bacterial liquid of bacillus alpina, streptomyces purpureus, geobacillus stearothermophilus, bacillus subtilis and bacillus licheniformis according to the volume ratio of 0.5: 0.5: 0.2: 0.5: 0.2 mixing, inoculating 8% fermentation medium volume ratio after high temperature sterilization, fermenting at 35 deg.C for 5 days until viable count reaches 1 × 108When the concentration is more than CFU/mL, the culture is completed. The fermentation medium is prepared by mixing the following raw materials in parts by weight: 15-25g of brown sugar, 5-10g of peptone, 3-5g of beef extract, 2-5g of NaCl and KH2PO4 0.75-1.5g、MgSO4.7H2O 0.5-1g、K2HPO40.8-1g, vitamin B110-15 mg, and 1L potato extractive solution (peeling potato 200g, cutting into small pieces, adding water 1L, boiling for 30min, filtering to remove potato pieces, adding filtrate to 1L), adjusting pH to 7, sterilizing at 121 deg.C for 30min, and cooling.
Example 2:
the method comprises the following steps of (1) mixing bacterial liquid of bacillus alpina, streptomyces purpureus, geobacillus stearothermophilus, bacillus subtilis and bacillus licheniformis according to the volume ratio of 0.8: 0.8: 0.5: 0.8: 0.5 mixing, inoculating with 10% volume of high temperature sterilized fermentation medium, fermenting at 35 deg.C for 5 days until viable count reaches 1 × 108When the concentration is more than CFU/mL, the culture is completed. The fermentation medium is prepared by mixing the following raw materials in parts by weight: 15-25g of brown sugar, 5-10g of peptone, 3-5g of beef extract, 2-5g of NaCl and KH2PO4 0.75-1.5g、MgSO4.7H2O 0.5-1g、K2HPO40.8-1g, vitamin B110-15 mg, and 1L potato extractive solution (peeled potato)200g, cutting into small pieces, adding 1L water, boiling for 30min, filtering to remove potato pieces, adding the filtrate to 1L), adjusting pH to 7, sterilizing at 121 deg.C for 30min, and cooling for use.
This embodiment is the most preferred embodiment.
Example 3:
the method comprises the following steps of (1) mixing bacterial liquid of bacillus alpina, streptomyces purpureus, geobacillus stearothermophilus, bacillus subtilis and bacillus licheniformis according to the volume ratio of 1: 1: 0.8: 1: 0.8 percent of the mixture is inoculated with 12 percent of the volume ratio of the fermentation medium sterilized at high temperature, and the mixture is fermented and cultured for 5 days at the temperature of 35 ℃, when the viable count reaches 1 multiplied by 108When the concentration is more than CFU/mL, the culture is completed. The fermentation medium is prepared by mixing the following raw materials in parts by weight: 15-25g of brown sugar, 5-10g of peptone, 3-5g of beef extract, 2-5g of NaCl and KH2PO4 0.75-1.5g、MgSO4.7H2O 0.5-1g、K2HPO40.8-1g, vitamin B110-15 mg, and 1L potato extractive solution (peeling potato 200g, cutting into small pieces, adding water 1L, boiling for 30min, filtering to remove potato pieces, adding filtrate to 1L), adjusting pH to 7, sterilizing at 121 deg.C for 30min, and cooling.
Example 4:
the method comprises the following steps of (1) mixing bacterial liquid of bacillus alpina, streptomyces purpureus, geobacillus stearothermophilus, bacillus subtilis and bacillus licheniformis according to the volume ratio of 0.6: 0.6: 0.4: 0.5: 0.4 mixing, inoculating with 12% volume of fermentation medium sterilized at high temperature, fermenting at 35 deg.C for 5 days until viable count reaches 1 × 108When the concentration is more than CFU/mL, the culture is completed. The fermentation medium is prepared by mixing the following raw materials in parts by weight: 15-25g of brown sugar, 5-10g of peptone, 3-5g of beef extract, 2-5g of NaCl and KH2PO4 0.75-1.5g、MgSO4.7H2O 0.5-1g、K2HPO40.8-1g, vitamin B110-15 mg, and 1L potato extractive solution (peeling potato 200g, cutting into small pieces, adding water 1L, boiling for 30min, filtering to remove potato pieces, adding filtrate to 1L), adjusting pH to 7, sterilizing at 121 deg.C for 30min, and cooling.
The use condition of the composite microbial inoculum prepared in the embodiment in sludge compost sheets is as follows:
the complex microbial inoculum prepared according to the above examples 1-4 is directly sprayed according to the ratio of 1: 500-600 is connected into the compost, when the compost is fermented and heated, the temperature is reduced, and finally the temperature is the same as the ambient temperature, so that the sludge composting is completed. The sludge is uniformly mixed with auxiliary materials before composting, the water content of the sludge is adjusted to be 50-60%, and the auxiliary materials are two or more than two mixtures of straws, sawdust, livestock and poultry manure, rice hulls and municipal garbage. Compared with sludge compost which is not inoculated with the composite microbial inoculum, the time of the compost reaching the high temperature stage is advanced by 5-7 days, the composting period is shortened by 12-16 days, the water content of the compost is reduced by 28-32%, the odor emission of the compost is reduced, the total nitrogen content in the compost is improved by 20-25%, and the sludge composting efficiency is improved.
TABLE 1 odor emission comparison table for sludge compost inoculated with the complex microbial inoculum of the invention and uninoculated compost
TABLE 2 temperature analysis of inoculated composite microbial inoculum of the invention and uninoculated compost in sludge compost
Table 3 basic index results of inoculated composite bacterial agent of the invention and uninoculated sludge compost product
Claims (3)
1. An application method of a composite microbial inoculum for sludge composting is characterized by comprising the following steps:
before composting sludge, mixing and stirring the compost and auxiliary materials uniformly, and adjusting the water content to 50-60%; according to the following steps: the mass ratio of 500-;
the auxiliary materials are a mixture of two or more than two of straws, sawdust, livestock and poultry manure, rice hulls and municipal garbage;
the complex microbial inoculum is prepared by the method comprising the following steps:
respectively and independently culturing bacillus altitudinis, streptomyces violaceus, geobacillus stearothermophilus, bacillus subtilis and bacillus licheniformis, mixing the bacillus altitudinis, the streptomyces violaceus, the streptomyces stearothermophilus, the bacillus subtilis and the bacillus licheniformis according to the volume proportion of bacteria liquid to obtain mixed bacteria liquid, and then inoculating the mixed bacteria liquid into a fermentation culture medium to perform amplification culture to obtain the composite microbial inoculum for sludge composting;
when in mixing, the volume ratio of the bacteria liquid of the geobacillus altitudinis, the streptomyces lividans, the geobacillus stearothermophilus, the bacillus subtilis and the bacillus licheniformis is 0.8: 0.8: 0.5: 0.8: 0.5;
the mixed bacterial liquid is inoculated into a fermentation medium for amplification culture at the culture temperature of 30-37 ℃, the culture time of 3-5d, the stirring speed of 160-200rpm, the ventilation volume of 1:1.5-2, the stirring interval time of 2h, the stirring time of 2min, when the viable count reaches 1 × 108And when the CFU/mL is higher than the set value, the preparation of the complex microbial inoculum is completed.
2. The method of application according to claim 1, characterized in that:
the fermentation medium is prepared by mixing the following raw materials in parts by weight: 15-25g of brown sugar, 5-10g of peptone, 3-5g of beef extract, 2-5g of NaCl and KH2PO4 0.75-1.5 g，MgSO4 .7H2O 0.5-1 g，K2HPO40.8-1g, vitamin B110-15 mg, and potato extractive solution 1L, with pH =7, sterilizing at 121 deg.C for 30min, and cooling.
3. The method of application according to claim 1, characterized in that:
the inoculation volume ratio of the mixed bacterial liquid inoculated into the fermentation medium is 5-15%.
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| CN110791442A (en) * | 2019-06-13 | 2020-02-14 | 山东省林业科学研究院 | Geobacillus altivelis with phosphate solubilizing function and application thereof |
| CN110272173A (en) * | 2019-06-25 | 2019-09-24 | 安徽理工大学 | A kind of processing method of sludge |
| CN110590431B (en) * | 2019-09-17 | 2022-09-06 | 湖北同惠生物工程有限公司 | Sludge treatment method for reducing nitrogen emission |
| CN111499432B (en) * | 2020-04-13 | 2022-07-22 | 山东省农业科学院农业资源与环境研究所 | Microbial remediation microbial inoculum for saline-alkali soil as well as preparation method and application of microbial remediation microbial inoculum |
| CN113816777A (en) * | 2021-01-14 | 2021-12-21 | 淮北师范大学 | Method for preparing ecological organic fertilizer from straw and chicken manure and prepared ecological organic fertilizer |
| CN113151121A (en) * | 2021-06-08 | 2021-07-23 | 德州蓝德再生资源有限公司 | Method for preparing compost microbial inoculum by using kitchen garbage and spiral shell pressing residues |
| CN114921356B (en) * | 2022-01-18 | 2024-04-02 | 金华康扬环境科技有限公司 | Household kitchen waste aerobic composting composite microbial agent and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10358535A1 (en) * | 2003-12-13 | 2005-07-14 | Henkel Kgaa | Hybrid enzymes with cationic binding domain |
| CN102690769A (en) * | 2012-06-01 | 2012-09-26 | 德州市元和农业科技开发有限责任公司 | Compound microbial bacterial preparation for efficiently degrading feces and preparation method and application of compound microbial bacterial preparation |
| CN102703350A (en) * | 2012-05-29 | 2012-10-03 | 北京大学 | Application of salt-tolerant nitrogen and phosphorus removing bacillus alitudinis to wastewater treatment |
| CN102765972A (en) * | 2012-07-17 | 2012-11-07 | 上海交通大学 | Organic garbage decomposing bacterium agent, as well as preparation method and application of organic garbage composting bacterium agent |
| CN104293694B (en) * | 2014-09-03 | 2017-10-31 | 广西博世科环保科技股份有限公司 | A kind of preparation method of sludge aerobic compost composite bacteria agent |
| CN107603909A (en) * | 2017-09-30 | 2018-01-19 | 河北天泓环保科技有限公司 | Sewage treatment microbial inoculant and its application method |
| CN108486010A (en) * | 2018-03-27 | 2018-09-04 | 中国科学院上海高等研究院 | A kind of During High-Temperature Composting bacterium composite bacteria agent and its application |
-
2018
- 2018-11-27 CN CN201811423120.7A patent/CN109385382B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10358535A1 (en) * | 2003-12-13 | 2005-07-14 | Henkel Kgaa | Hybrid enzymes with cationic binding domain |
| CN102703350A (en) * | 2012-05-29 | 2012-10-03 | 北京大学 | Application of salt-tolerant nitrogen and phosphorus removing bacillus alitudinis to wastewater treatment |
| CN102690769A (en) * | 2012-06-01 | 2012-09-26 | 德州市元和农业科技开发有限责任公司 | Compound microbial bacterial preparation for efficiently degrading feces and preparation method and application of compound microbial bacterial preparation |
| CN102765972A (en) * | 2012-07-17 | 2012-11-07 | 上海交通大学 | Organic garbage decomposing bacterium agent, as well as preparation method and application of organic garbage composting bacterium agent |
| CN104293694B (en) * | 2014-09-03 | 2017-10-31 | 广西博世科环保科技股份有限公司 | A kind of preparation method of sludge aerobic compost composite bacteria agent |
| CN107603909A (en) * | 2017-09-30 | 2018-01-19 | 河北天泓环保科技有限公司 | Sewage treatment microbial inoculant and its application method |
| CN108486010A (en) * | 2018-03-27 | 2018-09-04 | 中国科学院上海高等研究院 | A kind of During High-Temperature Composting bacterium composite bacteria agent and its application |
Non-Patent Citations (2)
| Title |
|---|
| 复合微生物菌剂对河道底泥资源化堆肥效果的影响;官昭瑛等;《广东农业科学》;20161215(第12期);33-35 * |
| 耐盐复合菌剂强化生物工艺处理高盐废水的快速启动;姜超等;《环境工程学报》;20170705(第07期);16-18 * |
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