CN109022306A - A kind of complex microbial inoculum and method and its application of efficient degradation grease - Google Patents
A kind of complex microbial inoculum and method and its application of efficient degradation grease Download PDFInfo
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- CN109022306A CN109022306A CN201810792925.2A CN201810792925A CN109022306A CN 109022306 A CN109022306 A CN 109022306A CN 201810792925 A CN201810792925 A CN 201810792925A CN 109022306 A CN109022306 A CN 109022306A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
- C02F2103/322—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters from vegetable oil production, e.g. olive oil production
Abstract
The invention belongs to microbial technology fields, specifically disclose the complex microbial inoculum and method and its application of a kind of efficient degradation grease.The complex microbial inoculum includes each strain of following mass ratio: bacillus licheniformis: bacillus subtilis: proteolysis candidiasis: candidiasis=5~10:10~20:10~15:5~20.Complex microbial inoculum of the present invention is seeded to 0.5~10% inoculum concentration after reacting 3d in oily sewage, oil and grease degradation rate is up to 90% or more.Complex microbial inoculum provided by the present invention can be applied to oily waste water processing, harmless treatment breeding waste resource utilization, can industrially carry out scale application, have many advantages, such as that investment is small, high-efficient, without secondary pollution.
Description
Technical field
The present invention relates to microbial technology fields, and in particular, to a kind of microorganism of efficient degradation grease is compound
Microbial inoculum and method and its application.
Background technique
With the continuous improvement of China's economic level and living standard, the oil wastewater of oil-fat plant and catering trade discharge
It measures increasing.Since fat and oil don't dissolve in water, the grease contained in sewage is easy to cause the blocking of pipeline.Direct emission oiliness is useless
Water can endanger aquatic ecosystem, seriously pollute ambient enviroment.
The common processing method of oily waste water mainly has physical method, chemical method and biological method.Currently, solving this
The most effectual way of problem is exactly first to make sewage by oil expeller, by most grease in sewage under the centrifugation of oil expeller
It separates.The grease separated can incinerate or be sent to soot burial, but such processing mode will lead to
The pollution of environment.Biochemical method oil wastewater, what is mainly utilized is that microorganism has certain oils degradation ability, can will be oily
Rouge is as carbon source and the energy, and the effect of the degradation enzyme systems such as lipase by generating in microorganism growth process is by grease hydrolysis
For glycerol, fatty acid, final decompose is oxidized to the metabolites such as water and carbon dioxide；Entire treatment process investment is small, at low cost,
It is high-efficient and without secondary pollution, become the hot spot studied both at home and abroad.
But current research is typically limited to only the exploration to single bacterial strain, but the degradation grease ability of single bacterial strain has
Limit, the grease being unable in efficient degradation waste water.Therefore, it is necessary to a kind of complex microbial inoculum be developed, to improve oily waste water
The degradation efficiency of middle grease.
Summary of the invention
The purpose of the invention is to overcome in the prior art single bacterial strain it is low to the degradation efficiency of grease in oily waste water
Defect, a kind of complex microbial inoculum is provided, by preferably compounding bacillus licheniformis, bacillus subtilis, proteolysis vacation silk
The ratio of saccharomycete and candidiasis, and the condition for grease of preferably degrading, can achieve the purpose that efficient degradation grease.
Another object of the present invention is to provide the preparation methods of mentioned microorganism composite bacteria agent.
Another object of the present invention is to provide mentioned microorganism composite bacteria agents to support in oily waste water processing, harmless treatment
Grow the application in utilization of waste as resource.
Another object of the present invention is to provide a kind of methods of efficient degradation grease.
To achieve the goals above, the present invention is achieved by following scheme:
A kind of complex microbial inoculum, each strain comprising following mass ratio: bacillus licheniformis: bacillus subtilis:
Proteolysis candidiasis: candidiasis=5~10:10~20:10~15:5~20.
Show nothing between strain by antagonistic effect between each strain in complex microbial inoculum provided by the present invention
Antagonism can be used in mixed way.By preferably compound bacillus licheniformis, bacillus subtilis, proteolysis candidiasis and
The ratio of candidiasis, and the condition for grease of preferably degrading, can achieve the purpose that efficient degradation grease.
Preferably, the complex microbial inoculum includes each strain of following mass ratio: bacillus licheniformis: withered grass gemma
Bacillus: proteolysis candidiasis: candidiasis=5~10:10~15:10~15:5~15.
It is highly preferred that the complex microbial inoculum includes each strain of following mass ratio: bacillus licheniformis: withered grass bud
Spore bacillus: proteolysis candidiasis: candidiasis=5~10:10~15:10~15:5~10.
It is highly preferred that the complex microbial inoculum includes each strain of following mass ratio: bacillus licheniformis: withered grass bud
Spore bacillus: proteolysis candidiasis: candidiasis=1:1:1:1.
Preferably, in the complex microbial inoculum micro organism quantity up to 1 × 108Cfu/mL or more.
The preparation method of mentioned microorganism composite bacteria agent includes the following steps: S1. respectively by bacillus licheniformis and withered grass
Bacillus is inoculated in beef-protein medium, in 30 DEG C of 48~72h of shaken cultivation；S2. respectively by proteolysis vacation silk ferment
Female bacterium and candidiasis are inoculated in PDA culture medium, in 30 DEG C of 48~72h of shaken cultivation；It S3. will be obtained by step S1 and S2
Bacillus licheniformis, bacillus subtilis, proteolysis candidiasis and candidiasis are respectively 5~10:10 according to mass ratio
~20:10~15:5~20 are uniformly mixed to get complex microbial inoculum；The complex microbial inoculum is liquid bacterial agent, pH
Value is 6~7.
Complex microbial inoculum of the present invention is seeded in oily sewage with 0.5~10% inoculum concentration and reacts 3d
Afterwards, oil and grease degradation rate is up to 90% or more.Therefore, mentioned microorganism composite bacteria agent is also claimed at oily waste water in the present invention
Application in reason, harmless treatment breeding waste resource utilization.
Preferably, the grease concentration in the oily sewage is 0.1~10% (v/v).
A kind of method that efficient degradation grease is also claimed in the present invention, including the following steps: will be described in any of the above-described
Complex microbial inoculum is that 0.5~10% (v/v) is inoculated in the oily waste water containing 0.1~10% (v/v) grease with inoculum concentration
In, in 20~40 DEG C of 2~4d of processing.
Preferably, the concentration of grease is 1% in the oily waste water, and the inoculum concentration of the complex microbial inoculum is 3%.
Preferably, the treatment conditions are 30 DEG C of culture 3d.
Preferably, before microbe inoculation composite bacteria agent, the initial pH value for first adjusting oily waste water is 4~10.
It is highly preferred that the initial pH value for first adjusting oily waste water is 7 before microbe inoculation composite bacteria agent.
Compared with prior art, the invention has the following advantages:
Complex microbial inoculum of the present invention is seeded in oily sewage with 0.5~10% inoculum concentration and reacts 3d
Afterwards, oil and grease degradation rate is up to 90% or more.Complex microbial inoculum provided by the present invention can be applied to oily waste water and handle, be harmless
Change processing breeding waste resource utilization, can industrially carry out scale application, has and put into small, high-efficient, nothing two
The advantages that secondary pollution.
Specific embodiment
The present invention is made combined with specific embodiments below and further being elaborated, the embodiment is served only for explaining this
Invention, is not intended to limit the scope of the present invention.Test method as used in the following examples is normal unless otherwise specified
Rule method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
Beef-protein medium: NaCl 5.0g, beef extract 5.0g, peptone 10.0g, pH7.0, distilled water
1000mL.121 DEG C of sterilizing 20min.
PDA culture medium: potato 200g, sucrose 20g, pH are naturally, distilled water 1000mL.121 DEG C of sterilizing 20min.
Degradation culture medium: (NH4)2SO40.2g, K2HPO42.0g, KH2PO42.0g, NaCl 2.0g, MgSO4·7H2O
0.1g, soya-bean oil 1.0mL, pH 7.0, distilled water 1000mL.
A kind of complex microbial inoculum of embodiment 1 and preparation method thereof
A kind of complex microbial inoculum includes following strain: bacillus licheniformis, bacillus subtilis, proteolysis vacation silk ferment
Female bacterium and candidiasis.Each strain quality is than as shown in table 1:
Each strain quality ratio in 1 complex microbial inoculum of table
| Bacillus licheniformis | Bacillus subtilis | Proteolysis candidiasis | Candidiasis | |
| Formula 1 | 5 | 10 | 10 | 5 |
| Formula 2 | 5 | 15 | 15 | 10 |
| Formula 3 | 10 | 10 | 10 | 10 |
| Formula 4 | 10 | 15 | 15 | 10 |
The preparation method of the complex microbial inoculum includes the following steps: S1. respectively by bacillus licheniformis and withered grass
Bacillus is inoculated in beef-protein medium, in 30 DEG C of 48~72h of shaken cultivation；S2. respectively by proteolysis vacation silk ferment
Female bacterium and candidiasis are inoculated in PDA culture medium, in 30 DEG C of 48~72h of shaken cultivation；It S3. will be obtained by step S1 and S2
Bacillus licheniformis, bacillus subtilis, proteolysis candidiasis and candidiasis mix according to the proportion in table 1 equal respectively
It is even to get complex microbial inoculum；The complex microbial inoculum is liquid bacterial agent, and pH value is 6~7.
Using viable bacteria counting method, statistics is formulated the micro organism quantity in 1~4 complex microbial inoculum, the results showed that, match
Micro organism quantity in the complex microbial inoculum of side 1~4 is up to 1 × 108Cfu/mL or more.Concrete outcome is shown in Table 2.
Micro organism quantity in the complex microbial inoculum of the formula of table 2 1~4
| Formula 1 | Formula 2 | Formula 3 | Formula 4 | |
| Micro organism quantity (cfu/mL) | 1×108 | 1×109 | 1×1011 | 1×1010 |
2 complex microbial inoculum of embodiment is degraded the ability test of grease at different conditions
The energy of degradation grease has been carried out under following different condition by the 3 preparing microorganism composite bacteria agent of formula of embodiment 1
Power test, detailed process is as follows:
1, composite bacteria agent degradation grease condition optimizing
After respectively will be for examination strain liquid culture 48h, seed liquor be made in compounding.Single factor experiment: it in 72h, adopts respectively
The influence to oil and grease degradation rate is detected with different inoculum concentrations, initial pH value, temperature and original soy oil content, revolving speed is
150r/min.Grease concentration is measured by sampling after 72h, calculates oil and grease degradation rate.
2, measuring method
The volumetric flask for taking 7 25mL, using soybean oil as solute, using petroleum ether as solvent.Accurately compound concentration is followed successively by
0.0, the series of samples of 0.2,0.4,0.6,0.8,1.0 μ L/mL.With ultraviolet specrophotometer, at 225nm wavelength, with 1cm stone
English cuvette, using petroleum ether as blank, the absorbance of the above-mentioned series of samples of sequentially determining.Using oil concentration as abscissa, with OD225
For ordinate, standard curve is made.
Extraction: the Liquid Culture sample (5mL) after taking 72h is used after adding 50% ammonium sulfate of 5mL to remove isolating protein
5000r/min speed is centrifuged 10min, and supernatant moves into separatory funnel, and 1+1 sulfuric acid 5mL is added to be acidified.It is cleaned with 10mL petroleum ether
After conical flask, separatory funnel is moved into, and sufficiently vibrate 3min, standing is allowed to be layered, and water layer is moved into sampling bottle, by petroleum
Ether moves into 50mL volumetric flask, then water layer is moved back in separatory funnel, repeats to extract once, after stratification with 10mL petroleum ether
Petroleum ether layer is moved into volumetric flask, is mixed with first time extract liquor, by volumetric flask constant volume to 50mL.
Oil and grease degradation rate in sample:
Degradation rate=(initial oil content-residual oil content)/initial oil content × 100%
Various grease 72h degradation rates are calculated, two-decimal is as a result retained.
3, test result
(1) influence of the inoculum concentration to composite bacteria agent deoiling effect
The culture of seed liquor is carried out, then equal proportion is mixed and made into composite bacteria agent.By 0.5%, 1%, 3%, 5% and 7%
Inoculum concentration be seeded in the 250mL conical flask equipped with 100mL degradation culture medium, be placed in 30 DEG C, cultivate in 150rpm shaking table
72h measures soybean grease degradation situation of the thallus in different vaccination amount.
The results are shown in Table 3, and when inoculum concentration is 3%, deoiling effect is best.
Influence of 3 inoculum concentration of table to composite bacteria agent deoiling effect
(2) initial pH influences the deoiling effect of composite bacteria agent
The culture of seed liquor is carried out, then equal proportion is mixed and made into composite bacteria agent.By step (1) resulting optimum inoculation amount
Be seeded to equipped with 100mL initial pH value be respectively 5,6,7,8 and 9 degradation culture medium 250mL conical flask in, be placed in 30 DEG C,
72h is cultivated in 150rpm shaking table, measures soybean grease degradation situation of the thallus in the initial pH of difference.
The results are shown in Table 4, and when the initial pH value for culture medium of degrading is 7.0, deoiling effect is best.
Table 4 degrades the initial pH of culture medium to the influence of the deoiling effect of composite bacteria agent
| Initial pH | Oil and grease degradation rate (%) |
| 5 | 82.35 |
| 6 | 82.50 |
| 7 | 84.14 |
| 8 | 83.02 |
| 9 | 81.90 |
(3) temperature influences the deoiling effect of composite bacteria agent
The culture of seed liquor is carried out, then equal proportion is mixed and made into composite bacteria agent.With step (1) resulting optimum inoculation amount
Be seeded to equipped with 100mL degradation culture medium 250mL conical flask in, be respectively placed under optimal initial pH value 20 DEG C, 25 DEG C,
72h is cultivated in 30 DEG C, 35 DEG C and 40 DEG C shaking tables, measures soybean grease degradation situation of the thallus in different temperatures.
The results are shown in Table 5, and when temperature is 30 DEG C, deoiling effect is best.
5 temperature of table influences the deoiling effect of composite bacteria agent
| Temperature (DEG C) | Oil and grease degradation rate (%) |
| 20 | 63.4163 |
| 25 | 74.2006 |
| 30 | 82.5716 |
| 35 | 76.463 |
| 40 | 74.6531 |
(4) influence of the different soybean grease concentration to composite bacteria agent deoiling effect
The culture of seed liquor is carried out, then equal proportion is mixed and made into composite bacteria agent.It is seeded to optimum inoculation amount equipped with nothing
In the 250mL conical flask of the 100mL degradation culture medium of oil, 0.01%, 0.05%, 0.1%, 0.15% and 0.2% is added respectively
Different amounts of soybean oil is as sole carbon source, after cultivating 72h under optimal initial pH value, optimum culturing temperature, measures thallus pair
The degradation situation of soybean grease.
The results are shown in Table 6, and when grease concentration is 1%, deoiling effect is best.
Influence of the different soybean grease concentration of table 6 to composite bacteria agent deoiling effect
| Grease concentration (%) | Oil and grease degradation rate (%) |
| 0.1 | 97.32 |
| 0.5 | 96.85 |
| 1 | 96.77 |
| 1.5 | 90.80 |
| 2 | 89.29 |
The optimal degradation condition of grease in conclusion complex microbial inoculum of the invention is degraded are as follows: initial grease concentration
It is 1%, inoculum concentration 3%, cultivation temperature is 30 DEG C, incubation time 3d, and the initial pH of oils degradation culture medium is 7.0.
Comparative example
A kind of complex microbial inoculum, each component mass ratio are as shown in table 7:
Each component mass ratio in 7 complex microbial inoculum of table
| Bacillus licheniformis | Bacillus subtilis | Proteolysis candidiasis | Candidiasis | Brewer's yeast | Pichia | |
| Formula 5 | 5 | 5 | 10 | / | / | / |
| Formula 6 | 5 | 10 | / | 5 | / | / |
| Formula 7 | 15 | 10 | 20 | 25 | / | / |
| Formula 8 | 10 | 20 | 5 | 15 | / | / |
| Formula 9 | 10 | 10 | 10 | / | 10 | / |
| Formula 10 | 10 | 10 | 10 | / | / | 10 |
The preparation method step of the complex microbial inoculum is roughly the same with embodiment 1.
Using viable bacteria counting method, statistics is formulated the micro organism quantity in 5~10 complex microbial inoculum, the results showed that,
Micro organism quantity in the complex microbial inoculum of formula 5~10 is 1 × 107~1 × 108cfu/mL.Concrete outcome is shown in Table 8.
Micro organism quantity in the complex microbial inoculum of the formula of table 8 5~8
| Formula 5 | Formula 6 | Formula 7 | Formula 8 | Formula 9 | Formula 10 | |
| Micro organism quantity (cfu/mL) | 2.4×107 | 3.9×107 | 8.6×107 | 7.0×107 | 9.0×107 | 9.5×107 |
The complex microbial inoculum of formula 5~10 is seeded to the 250mL conical flask equipped with 100mL degradation culture medium respectively
In, after being cultivated using the best degradation condition of 2 gained of embodiment, degradation situation of the measurement thallus to soybean grease.
The results are shown in Table 9, and the deoiling effect for being formulated 5~10 complex microbial inoculums is poor, cannot efficiently drop in 72h
Solve soybean grease.
Table 9 is formulated the deoiling effect of 5~10 complex microbial inoculums
| Formula | Oil and grease degradation rate (%) |
| 5 | 56.46 |
| 6 | 53.21 |
| 7 | 65.18 |
| 8 | 60.49 |
| 9 | 44.67 |
| 10 | 37.84 |
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of shield range can also be made on the basis of above description and thinking for those of ordinary skill in the art
Other various forms of variations or variation, there is no necessity and possibility to exhaust all the enbodiments.It is all of the invention
Made any modifications, equivalent replacements, and improvements etc., should be included in the protection of the claims in the present invention within spirit and principle
Within the scope of.
Claims (10)
1. a kind of complex microbial inoculum, which is characterized in that each strain comprising following mass ratio: bacillus licheniformis: withered grass
Bacillus: proteolysis candidiasis: candidiasis=5~10:10~20:10~15:5~20.
2. complex microbial inoculum according to claim 1, which is characterized in that each strain comprising following mass ratio: ground
Clothing bacillus: bacillus subtilis: proteolysis candidiasis: candidiasis=5~10:10~15:10~15:5~15.
3. complex microbial inoculum according to claim 2, which is characterized in that each strain comprising following mass ratio: ground
Clothing bacillus: bacillus subtilis: proteolysis candidiasis: candidiasis=5~10:10~15:10~15:5~10.
4. described in any item complex microbial inoculums according to claim 1~3, which is characterized in that the microbial composite bacteria
Micro organism quantity is up to 1 × 10 in agent8Cfu/mL or more.
5. the preparation method of any one of claims 1 to 3 complex microbial inoculum, which comprises the steps of:
S1. bacillus licheniformis and bacillus subtilis are inoculated in beef-protein medium respectively, are vibrated in 30 DEG C
Cultivate 48~72h；
S2. proteolysis candidiasis and candidiasis are inoculated in PDA culture medium respectively, in 30 DEG C of shaken cultivations 48~
72h；
S3. by bacillus licheniformis obtained by step S1 and S2, bacillus subtilis, proteolysis candidiasis and candidiasis point
It is not that 5~10:10~20:10~15:5~20 are uniformly mixed to get complex microbial inoculum according to mass ratio；The microorganism
Composite bacteria agent is liquid bacterial agent, and pH value is 6~7.
6. any one of claims 1 to 3 complex microbial inoculum is in oily waste water processing, harmless treatment breeding waste
Application in resource utilization.
7. a kind of method of efficient degradation grease, which comprises the steps of: will be described in any one of claims 1 to 3
Complex microbial inoculum is with inoculum concentration for 0.5~10%(v/v) be inoculated in containing 0.1~10%(v/v) in the oily waste water of grease,
In 20~40 DEG C of 2~4d of processing.
8. the method according to the description of claim 7 is characterized in that in the oily waste water grease concentration be 1%, it is described micro-
The inoculum concentration of biological compound fungi agent is 3%.
9. the method according to the description of claim 7 is characterized in that the treatment conditions are 30 DEG C of culture 3d.
10. the method according to the description of claim 7 is characterized in that it is useless first to adjust oiliness before microbe inoculation composite bacteria agent
The initial pH value of water is 4~10.
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| CN110980960A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Oil-removing microbial inoculum and sludge treatment technology using same |
| CN111849821A (en) * | 2020-07-28 | 2020-10-30 | 浙江工业大学 | High-temperature-resistant kitchen waste grease degradation compound microbial inoculum and application thereof |
| CN112094782A (en) * | 2020-09-27 | 2020-12-18 | 深圳市家家分类环保技术开发有限公司 | Composite microbial inoculum for degrading kitchen garbage and preparation method thereof |
| CN113061556A (en) * | 2021-04-23 | 2021-07-02 | 上海乾界生物科技有限公司 | Lipid-decomposing bacteria preparation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110980960A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Oil-removing microbial inoculum and sludge treatment technology using same |
| CN111849821A (en) * | 2020-07-28 | 2020-10-30 | 浙江工业大学 | High-temperature-resistant kitchen waste grease degradation compound microbial inoculum and application thereof |
| CN112094782A (en) * | 2020-09-27 | 2020-12-18 | 深圳市家家分类环保技术开发有限公司 | Composite microbial inoculum for degrading kitchen garbage and preparation method thereof |
| CN113061556A (en) * | 2021-04-23 | 2021-07-02 | 上海乾界生物科技有限公司 | Lipid-decomposing bacteria preparation |
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