CN108192840A - For the microbial inoculum and preparation method of organic waste matter fermentation process - Google Patents
For the microbial inoculum and preparation method of organic waste matter fermentation process Download PDFInfo
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- CN108192840A CN108192840A CN201810024098.2A CN201810024098A CN108192840A CN 108192840 A CN108192840 A CN 108192840A CN 201810024098 A CN201810024098 A CN 201810024098A CN 108192840 A CN108192840 A CN 108192840A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention belongs to organic waste processing technology fields.The technical issues of fermentation is slow in the prior art for solution, needs artificially to stir, and fermentation process organic waste is easily corrupt, a kind of microbial inoculum and preparation method for organic waste matter fermentation process of present invention offer, the microbial inoculum include liquid bacterial agent；The liquid bacterial agent includes the component of following parts by weight：0.005 0.015 parts of beef extract, 0.02 0.04 parts of peptone, 0.04 0.06 parts of glucose, 0.002 0.006 parts of sodium chloride, 101.2 101.8 parts of distilled water, 24 parts of egg white, 0.05 0.2 parts of sheep serum, 24 parts of brown sugar, 0.02 0.06 parts of the metabolite of photosynthetic bacteria, 0.02 0.06 parts of the metabolite of saccharomycete, 0.2 0.6 parts of EM bacterium；Further include carrier；The weight ratio of the carrier and liquid bacterial agent is 20~30:1, the carrier is the organic particle that mesh number is 10~20 mesh.The present invention have save manpower, extend fermentation time, improve fermentation efficiency, suitable for aerobic fermentation difficulty high organic waste the characteristics of.
Description
Technical field
The invention belongs to organic waste processing technology fields, and in particular to one kind is used for organic waste matter fermentation process
Microbial inoculum and preparation method.
Background technology
At present, with urban development, population increase, the yield rate of climb of organic waste quickly, how expeditiously
The problem of these organic wastes are carried out with " minimizing, innoxious, recycling " processing, becomes social common concern.Wherein, have
Machine house refuse, kitchen garbage, food processing industry leftover bits and pieces, Organic sludge and fowl and animal excrement etc. are respectively provided with the content of organic matter
The general character such as high, biodegradable, are referred to as biodegradable organic waste.
At present, processing of the people for garbage and organic waste, frequently with stack retting facture, combustion gasification method, overcritical
Water oxidation, power generation by waste combustion pattern Anaerobic Digestion method etc., but there is occupied ground is big, Air-pollution From Combustion environment, waste money
The drawbacks such as source.And the organic wastes such as municipal sludge deodorization, aerobic fermentation processing, stalk, feces of livestock and poultry are generally adopted
With the mode of biological treatment, i.e., the organic substance in organic waste is decomposed by zymophyte.
It is however, often not ideal enough for the microbial inoculum of cultivation and fermentation bacterium in existing biological treatment mode.With microorganism
Activity, the temperature of microbial inoculum constantly changes, since existing microbial inoculum overall air permeability is poor, cause heat it is each part collection
It is poly-, so as to cause the raising of local temperature, zymogenic activity is influenced, so as to influence its decomposition to organic substance, so as to lead
Cause its discomposing effect poor, and existing microbial inoculum is often only conducive to single growth, when part is too high or too low for temperature
When, large effect can be generated to zymophyte or even can stop fermenting, so as to reduce fermentation efficiency；In addition, due to processing procedure
The regular hour is needed, easily corruption generates stink to the organic substance discarded in processing procedure.
Invention content
It is an object of the invention to solve technical problem in the prior art above at least one of, a kind of use is provided
In the microbial inoculum and preparation method of organic waste matter fermentation process.
The purpose of the present invention is what is be achieved through the following technical solutions：
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：0.005-0.015 parts of beef extract, 0.02-0.04 parts of peptone, 0.04-0.06 parts of glucose, sodium chloride
0.002-0.006 parts, 101.2-101.8 parts of distilled water, 2-4 parts of egg white, 0.05-0.2 parts of sheep serum, 2-4 parts of brown sugar, light
Close 0.02-0.06 parts of the metabolite of bacterium, 0.02-0.06 parts of the metabolite of saccharomycete, 0.2-0.6 parts of EM bacterium；Further include load
Body；The weight ratio of the carrier and liquid bacterial agent is 20~30:1, the carrier is the organic matter that mesh number is 10~20 mesh
Grain.
Further, the organic particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt
Powder, each equal portions of old half meal and old face.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：1.2-1.8 parts of beef extract, peptone, glucose, sodium chloride distilled water are mixed equal
After even, pH value is adjusted to 7~8, sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
A kind of embodiment is to further include step C：C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed to carrier table
On face, fertilizer zymophyte is obtained.
Another embodiment is to further include step D：Prepare fertilizer zymophyte：After liquid bacteria and carrier mixing, obtain
Immobilized zymophyte；By the immobilized zymophyte, cook, it is dry, obtain fertilizer zymophyte after broken.
Further, step S is further included before the step C：S, carrier is prepared：By dregs of beans, corn flour, wheat bran, malt
It is dry by cooking after powder, old half meal and the mixing of old face, obtain particulate matter carrier after broken.
Further, the carrier of the step C and the weight ratio of liquid bacterium are 20:1.
Further, the mesh number of the particulate matter carrier of the step C is 20 mesh.
Further, the carrier of the step D and the weight ratio of liquid bacterium are 20:The particulate matter of 1, the step D carry
The mesh number of body is 20 mesh.
The present invention is relative to the advantageous effect of the prior art：The microbial inoculum for organic waste fermentation process of the present invention
And preparation method, by liquid bacterial agent being sprayed on the particulate matter carrier for the irregular particle shape for being used as carrier be made fertilizer hair
Yeast-like fungi, the condition for making its fermentation time longer and fermenting space are provided for aerobic bacteria, and aerobic bacteria is carried in irregular particulate matter
It is sufficient for fermentation activity 5 days or more under the oxygen that gap between body is provided, so as to extend fermentation time, gives birth to city
The organic wastes such as sludge living decompose more thorough；The microbial inoculum of the present invention is competed using microorganism and restricted by using EM bacterium
The characteristic mutually eaten promotes zoogloea growth, and provides suitable condition for wherein three classes bacterium, to establish dominant microflora；In microorganism
Temperature gradually rises during continuously active, and under the protection of nutritional agents in a dormant state, high temperature bacterium is then movable for mesophile
Start to cool down after a period of time, mesophile immediately engages in activity after reaching mesophile appropriate state, and organic materials is fermenting repeatedly
In the process, the principle expanded with heat and contract with cold using material and evaporate the space that moisture is vacateed, along between fertilizer zymophyte this
The gap that body has provides more activity spaces and oxygen for zymophyte, ensure that the condition of zymophyte continuously active, carry
The condition of organic fertilizer ageing compost has been supplied, while has inhibited the activity of anaerobism class harmful bacteria, it is therefore prevented that the corruption of organic waste
It loses, while reduces the artificial operation stirred and increase oxygen mixed volume, save manpower；The microbial inoculum of the present invention is particularly suitable for city
City's domestic sludge isodensity is bigger, high organic waste matter such as feces of livestock and poultry of aerobic fermentation difficulty etc..
Specific embodiment
Technical scheme of the present invention is described in detail with reference to embodiments, it should be noted that be only below
The preferred embodiment of the present invention, for those of ordinary skill in the art, before the invention design is not departed from
It puts, various modifications and improvements can be made, these should belong to protection scope of the present invention.
Old half meal refers to the rice by spontaneous fermentation in the present invention, is obtained after being placed 1-2 days by cooked rice.
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：0.005-0.015 parts of beef extract, 0.02-0.04 parts of peptone, 0.04-0.06 parts of glucose, sodium chloride
0.002-0.006 parts, 101.2-101.8 parts of distilled water, 2-4 parts of egg white, 0.05-0.2 parts of sheep serum, 2-4 parts of brown sugar, light
Close 0.02-0.06 parts of the metabolite of bacterium, 0.02-0.06 parts of the metabolite of saccharomycete, 0.2-0.6 parts of EM bacterium；Further include load
Body；The weight ratio of the carrier and liquid bacterial agent is 20~30:1, the carrier is the organic matter that mesh number is 10~20 mesh
Grain.
Preferably, the organic particle is irregular shape, so as to offer oxygen space as much as possible.
Further, the organic particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt
Powder, each equal portions of old half meal and old face.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：1.2-1.8 parts of beef extract, peptone, glucose, sodium chloride distilled water are mixed equal
After even, pH value is adjusted to 7~8, sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
A kind of embodiment is to further include step C：C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed to carrier table
On face, fertilizer zymophyte is obtained.
Further, step S is further included before the step C：S, carrier is prepared：By dregs of beans, corn flour, wheat bran, malt
It is dry by cooking after powder, old half meal and the mixing of old face, obtain particulate matter carrier after broken.
Preferably, dry using low temperature drying, the low temperature is the temperature less than room temperature (20~25 DEG C), and in this temperature
Under, it is air-dried or is dried in the shade.
Further, the carrier of the step C and the weight ratio of liquid bacterium are 20:1.
Further, the mesh number of the particulate matter carrier of the step C is 20 mesh.
Another embodiment is to further include step D：Prepare fertilizer zymophyte：After liquid bacteria and carrier mixing, obtain
Immobilized zymophyte；By the immobilized zymophyte, cook, it is dry, obtain fertilizer zymophyte after broken.
Preferably, dry using low temperature drying, the low temperature is the temperature less than room temperature (20~25 DEG C), and in this temperature
Under, it is air-dried or is dried in the shade.
The carrier of the step D is 20 with the weight ratio of liquid bacterium:1, the mesh number of the particulate matter carrier of the step D is
20 mesh.
Embodiment 1
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：0.005 part of beef extract, 0.02 part of peptone, 0.04 part of glucose, 0.002 part of sodium chloride, distilled water
101.2 parts, 2 parts of egg white, 0.05 part of sheep serum, 2 parts of brown sugar, 0.02 part of the metabolite of photosynthetic bacteria, the metabolism of saccharomycete
0.02 part of substance, 0.2 part of EM bacterium.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：Beef extract, peptone, glucose, sodium chloride are uniformly mixed with 1.2 parts of distilled water
Afterwards, pH value is adjusted to 7, and sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
Further include step C：C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed on the surface of the carrier, obtains fertilizer hair
Yeast-like fungi.
Embodiment 2
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：0.015 part of beef extract, 0.04 part of peptone, 0.06 part of glucose, 0.006 part of sodium chloride, distilled water
101.8 parts, 4 parts of egg white, 0.2 part of sheep serum, 4 parts of brown sugar, 0.06 part of the metabolite of photosynthetic bacteria, the metabolin of saccharomycete
0.06 part of matter, 0.6 part of EM bacterium.
For the microbial inoculum of organic waste matter fermentation process, carrier is further included；The weight ratio of the carrier and liquid bacterial agent
Example is 20:1, the carrier is the organic particle that mesh number is 10 mesh.
Organic particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
Each equal portions in face.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：Beef extract, peptone, glucose, sodium chloride are uniformly mixed with 1.8 parts of distilled water
Afterwards, pH value is adjusted to 8, and sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed on the surface of the carrier, obtains fertilizer zymophyte.
Step S is further included before step C：S, carrier is prepared：By dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
It is dry by cooking after the mixing of face, obtain particulate matter carrier after broken.
The carrier of the step C is 20 with the weight ratio of liquid bacterium:1.
Embodiment 3
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：10 parts of beef extract, 0.03 part of peptone, 0.05 part of glucose, 0.004 part of sodium chloride, distilled water 101.5
Part, 3 parts of egg white, 0.1 part of sheep serum, 3 parts of brown sugar, 0.05 part of the metabolite of photosynthetic bacteria, the metabolite of saccharomycete
0.05 part, 0.5 part of EM bacterium.
For the microbial inoculum of organic waste matter fermentation process, carrier is further included；The weight ratio of the carrier and liquid bacterial agent
Example is 30:1, the carrier is the organic particle that mesh number is 20 mesh.
Organic particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
Each equal portions in face.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：Beef extract, peptone, glucose, sodium chloride are uniformly mixed with 1.5 parts of distilled water
Afterwards, pH value is adjusted to 7.2, and sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed on the surface of the carrier, obtains fertilizer zymophyte.
Step S is further included before the step C：S, carrier is prepared：By dregs of beans, corn flour, wheat bran, malt flour, old half meal
It is dry by cooking after being mixed with old face, obtain particulate matter carrier after broken.
The mesh number of the particulate matter carrier of the step C is 20 mesh.
Embodiment 4
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：0.005 part of beef extract, 0.02 part of peptone, 0.04 part of glucose, 0.002 part of sodium chloride, distilled water
101.2 parts, 2 parts of egg white, 0.05 part of sheep serum, 2 parts of brown sugar, 0.02 part of the metabolite of photosynthetic bacteria, the metabolism of saccharomycete
0.02 part of substance, 0.2 part of EM bacterium.
For the microbial inoculum of organic waste matter fermentation process, carrier is further included；The weight ratio of the carrier and liquid bacterial agent
Example is 25:1, the carrier is the organic particle that mesh number is 15 mesh.
Organic particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
Each equal portions in face.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：Beef extract, peptone, glucose, sodium chloride are uniformly mixed with 1.5 parts of distilled water
Afterwards, pH value is adjusted to 7.2, and sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed on the surface of the carrier, obtains fertilizer zymophyte.
Step S is further included before the step C：S, carrier is prepared：By dregs of beans, corn flour, wheat bran, malt flour, old half meal
It is dry by cooking after being mixed with old face, obtain particulate matter carrier after broken.
The carrier of the step C is 25 with the weight ratio of liquid bacterium:1.
The mesh number of the particulate matter carrier of the step C is 15 mesh.
Embodiment 5
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：0.015 part of beef extract, 0.04 part of peptone, 0.05 part of glucose, 0.0033 part of sodium chloride, distilled water
101.8 parts, 4 parts of egg white, 0.2 part of sheep serum, 4 parts of brown sugar, 0.06 part of the metabolite of photosynthetic bacteria, the metabolin of saccharomycete
0.06 part of matter, 0.6 part of EM bacterium.
For the microbial inoculum of organic waste matter fermentation process, carrier is further included；The weight ratio of the carrier and liquid bacterial agent
Example is 20:1, the carrier is the organic particle that mesh number is 20 mesh.
Organic particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
Each equal portions in face.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：Beef extract, peptone, glucose, sodium chloride are uniformly mixed with 1.8 parts of distilled water
Afterwards, pH value is adjusted to 7.5, and sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed on the surface of the carrier, obtains fertilizer zymophyte.
Step S is further included before step C：S, carrier is prepared：By dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
It is dry by cooking after the mixing of face, obtain particulate matter carrier after broken.
The carrier of the step C is 20 with the weight ratio of liquid bacterium:1.
The mesh number of the particulate matter carrier of the step C is 20 mesh.
Embodiment 6
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：10 parts of beef extract, 0.03 part of peptone, 0.05 part of glucose, 0.0045 part of sodium chloride, distilled water
101.5 parts, 3 parts of egg white, 0.1 part of sheep serum, 3 parts of brown sugar, 0.05 part of the metabolite of photosynthetic bacteria, the metabolin of saccharomycete
0.05 part of matter, 0.5 part of EM bacterium.
The microbial inoculum for organic waste matter fermentation process, further includes carrier；The carrier and liquid bacterial agent
Weight ratio is 20:1, the carrier is the organic particle that mesh number is 20 mesh.
Organic particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
Each equal portions in face.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：Beef extract, peptone, glucose, sodium chloride are uniformly mixed with 1.5 parts of distilled water
Afterwards, pH value is adjusted to 7.2, and sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed on the surface of the carrier, obtains fertilizer zymophyte.
Step S is further included before step C：S, carrier is prepared：By dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
It is dry by cooking after the mixing of face, obtain particulate matter carrier after broken.
The carrier of step C is 20 with the weight ratio of liquid bacterium:1.
The mesh number of the particulate matter carrier of step C is 20 mesh.
Embodiment 7
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：10 parts of beef extract, 0.03 part of peptone, 0.05 part of glucose, 0.0045 part of sodium chloride, distilled water
101.5 parts, 3 parts of egg white, 0.1 part of sheep serum, 3 parts of brown sugar, 0.05 part of the metabolite of photosynthetic bacteria, the metabolin of saccharomycete
0.05 part of matter, 0.5 part of EM bacterium.
The microbial inoculum for organic waste matter fermentation process, further includes carrier；The carrier and liquid bacterial agent
Weight ratio is 20:1, the carrier is the organic particle that mesh number is 20 mesh.
Organic particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
Each equal portions in face.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：Beef extract, peptone, glucose, sodium chloride are uniformly mixed with 1.5 parts of distilled water
Afterwards, pH value is adjusted to 7.2, and sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
Further include step D：Prepare fertilizer zymophyte：After liquid bacteria and carrier mixing, immobilized zymophyte is obtained；By described in
Immobilized zymophyte, cooks, dry, and fertilizer zymophyte is obtained after broken.
Embodiment 8
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
The component of parts by weight：10 parts of beef extract, 0.03 part of peptone, 0.05 part of glucose, 0.0045 part of sodium chloride, distilled water
101.5 parts, 3 parts of egg white, 0.1 part of sheep serum, 3 parts of brown sugar, 0.05 part of the metabolite of photosynthetic bacteria, the metabolin of saccharomycete
0.05 part of matter, 0.5 part of EM bacterium.
The microbial inoculum for organic waste matter fermentation process, further includes carrier；The carrier and liquid bacterial agent
Weight ratio is 20:1, the carrier is the organic particle that mesh number is 20 mesh.
Organic particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
Each equal portions in face.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：Beef extract, peptone, glucose, sodium chloride are uniformly mixed with 1.5 parts of distilled water
Afterwards, pH value is adjusted to 7.2, and sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, obtain liquid bacterium；
Further include step D：Prepare fertilizer zymophyte：After liquid bacteria and carrier mixing, immobilized zymophyte is obtained；By described in
Immobilized zymophyte, cooks, dry, and fertilizer zymophyte is obtained after broken.
The carrier of step D is 20 with the weight ratio of liquid bacterium:1.
The mesh number of the particulate matter carrier of step D is 20 mesh.
Embodiment 9
A kind of microbial inoculum for organic waste matter fermentation process, including liquid bacterial agent；The liquid bacterial agent includes as follows
Component：10 grams of beef extract, 30 grams of peptone, 50 grams of glucose, 4.5 grams of sodium chloride, 101.5 kilograms of distilled water, egg white 3,000
Gram, 0.1 kilogram of sheep serum, 3 kilograms of brown sugar, 50 grams of the metabolite of photosynthetic bacteria, 50 grams of the metabolite of saccharomycete, EM bacterium
500 grams.
The preparation method of a kind of microbial inoculum for organic waste matter fermentation process, including step：
A, sterile medium is prepared：By two bottles of 10 grams of beef extract, 30 grams of peptone, Dextrose and Sodium Chloride Inj. (specifications
For 500mL：Contain glucose 25g and sodium chloride 2.25g) and distilled water 500mL be uniformly mixed, adjust pH value to 7.2, in high temperature
After sterilizing 30 minutes (higher than the temperature of mixture boiling point, more than one atmospheric pressure) under high pressure, sterile medium is obtained；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, yeast
The metabolite of bacterium and EM bacterium mixings, fermentation, liquid bacterium is obtained after one month；
C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed on the surface of the carrier, obtains fertilizer zymophyte.
Step S is further included before step C：S, carrier is prepared：By dregs of beans, corn flour, wheat bran, malt flour, old half meal and old
After the mixing of face, cooked after 1 hour by high temperature, then its water content is down to by the mode of low temperature drying and accounts for the 15% of gross weight
Within, obtain particulate matter carrier after being broken into 20 mesh.The carrier of step C is 20 with the weight ratio of liquid bacterium:1.
Contrast test example
The microbial inoculum of 1-9 of the embodiment of the present invention any one embodiment is opposed with the corresponding microbial inoculums of other production men production
Than handling respectively municipal sludge, in use, by the corresponding microbial inoculum of each production man and the fertilizer zymophyte point of the present invention
Do not distinguish mixing with the municipal sludge from same area, carry out fermentation process, each index comparison is specifically shown in the following table 1.
The microbial inoculum product contrast table of 1 each company of table
To sum up, the microbial inoculum of the microbial inoculum of the present invention and each company was compared it is found that the fertilizer zymophyte heating-up time of the present invention
It is short, 70 DEG C can be risen to interior for 24 hours, and do not need to often stir the mixing of zymophyte and municipal sludge during the fermentation
Object, to increase oxygen content therein, and the zymophyte of three companies of contrast groups is when stirring, and fermentation situation is poor, even
Stop fermentation；On the other hand, the temperature of three company's mixtures of contrast groups, which rises to 65 DEG C, needs 72h, and the present invention only needs for 24 hours,
And the present invention establishes flora advantage in the mixture after 72h, so as to which the present invention is close suitable for municipal sludge etc.
Spend bigger, the high organic waste matter of aerobic fermentation difficulty.
Technical scheme of the present invention can preferably be realized according to the record of this specification.
Claims (9)
1. a kind of microbial inoculum for organic waste matter fermentation process, which is characterized in that including liquid bacterial agent；The liquid bacterial agent
Include the component of following parts by weight：0.005-0.015 parts of beef extract, 0.02-0.04 parts of peptone, glucose 0.04-0.06
Part, 0.002-0.006 parts of sodium chloride, 101.2-101.8 parts of distilled water, 2-4 parts of egg white, 0.05-0.2 parts of sheep serum are red
It is 2-4 parts sugared, 0.02-0.06 parts of the metabolite of photosynthetic bacteria, 0.02-0.06 parts of the metabolite of saccharomycete, EM bacterium 0.2-0.6
Part；Further include carrier；The weight ratio of the carrier and liquid bacterial agent is 20~30:1, the carrier is that mesh number is 10~20 mesh
Organic particle.
2. the microbial inoculum according to claim 1 for organic waste matter fermentation process, which is characterized in that the organic matter
Particle is made of the component of following parts by weight：Dregs of beans, corn flour, wheat bran, malt flour, each equal portions of old half meal and old face.
3. a kind of preparation method of the microbial inoculum for organic waste matter fermentation process described in claim 2, which is characterized in that
Including step：
A, sterile medium is prepared：Beef extract, peptone, glucose, sodium chloride are uniformly mixed with 1.2-1.8 parts of distilled water
Afterwards, pH value is adjusted to 7~8, and sterilizing obtains sterile medium；
B, liquid bacterium is prepared：By sterile medium, egg white, sheep serum, brown sugar, the metabolite of photosynthetic bacteria, saccharomycete
Metabolite and EM bacterium mixings, fermentation, obtain liquid bacterium.
4. the preparation method of the microbial inoculum according to claim 3 for organic waste matter fermentation process, which is characterized in that
Further include step C：C, fertilizer zymophyte is prepared：Liquid bacteria is uniformly sprayed on the surface of the carrier, obtains fertilizer zymophyte.
5. the preparation method of the microbial inoculum according to claim 4 for organic waste matter fermentation process, which is characterized in that
Step S is further included before the step C：S, carrier is prepared：Dregs of beans, corn flour, wheat bran, malt flour, old half meal and old face are mixed
It is dry by cooking after conjunction, obtain particulate matter carrier after broken.
6. the preparation method of the microbial inoculum according to claim 5 for organic waste matter fermentation process, which is characterized in that
The carrier of the step C is 20 with the weight ratio of liquid bacterium:1.
7. the preparation method of the microbial inoculum according to claim 5 for organic waste matter fermentation process, which is characterized in that
The mesh number of the particulate matter carrier of the step C is 20 mesh.
8. the preparation method of the microbial inoculum according to claim 4 for organic waste matter fermentation process, which is characterized in that
Further include step D：Prepare fertilizer zymophyte：After liquid bacteria and carrier mixing, immobilized zymophyte is obtained；By the immobilized fermentation
Bacterium cooks, dry, and fertilizer zymophyte is obtained after broken.
9. the preparation method of the microbial inoculum according to claim 8 for organic waste matter fermentation process, which is characterized in that
The carrier of the step D is 20 with the weight ratio of liquid bacterium:1, the mesh number of the particulate matter carrier of the step D is 20 mesh.
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