A kind of new process of aerobic fermentation processing chicken manure
Technical field
It is especially a kind of to utilize a variety of probiotics collaboration hair the present invention relates to a kind of new process of aerobic fermentation processing chicken manure
The preparations such as ferment chicken manure, stalk have effects that the technique of good growth-promoting, the bio-organic fertilizer increased production, belong to bioengineering field.
Background technique
With the development of Modern Animal Husbandry, poultry farms scale, intensive degree are continuously improved, large-scale poultry farming
The excrement that field generates seriously pollutes environment.State Environmental Protection Administration shows scale animal and poultry cultivation Survey of contamination status of sea products result in 2002
Show: Chinese festiva feces of livestock and poultry amount is about 1,900,000,000 tons, wherein COD(COD) discharge amount oneself up to 71,180,000 tons, feces of livestock and poultry
Yield and COD discharge amount are all considerably beyond the sum of industrial wastewater and discharging life water amount, and feces of livestock and poultry total amount in 2004 is
2800000000 tons;Reach 4,500,000,000 tons within 2010.This is more and more tight to the pollution of the mankind, other biologies and poultry own existence environment
Weight.From the point of view of environment, it causes serious pollution to atmosphere, waters and soil, deteriorates ball ecological environment.From resource
From the point of view of, it is good renewable resource, as being translated into fertilizer, realizes agriculture and animal husbandry recycling economy development, not only may be used
To solve two hang-up of shortage of resources and environmental pollution of current facing mankind.
The alimentary canal of chicken is short, and the feed residence time is not grown, and excrement gross protein value is high, and rich in a variety of amino acid,
Calcium, phosphorus and vitamin etc. have result of study to show in chicken manure dry matter, and crude protein 28.0%, can digest egg at pure protein 11.3%
White matter 14.4%, crude fat 2% or so, crude fibre 10% or so, calcium 6%-8%, phosphorus 2.5%-3.0%, also containing zinc, copper,
The mineral elements such as iron and B family vitamin abundant.Therefore, chicken manure is converted to using fertilizer by processing is a kind of comparison
Ideal Harmless processing mode.
During High-Temperature Composting is to realize the effective measures of livestock and poultry cultivation night soil harmless treatment, prepares chicken by During High-Temperature Composting technology
Excrement bio-organic fertilizer has been reported that more, if patent 201210481607.7 discloses a kind of fowl dung fermentation fertilizer, by dried poultrymanure, chaff
Powder, flue-cured tobacco stalk, effective microorganisms(EM), basalt powder, tea shell powder are prepared by normal temperature fermentation, triple mixing, gradation addition;Patent
201410493139.4 disclose a kind of production method of chicken manure biological organic fertilizer, and this method belongs to biological organic fertilizer field, makes
Raw material includes: dried poultrymanure 45-50%, the first fermenting microbe 0.5%, the second fermenting microbe 0.5%, cake fertilizer 40-45%, sludge
12%, organic fertilizer fermentation agent 1%, bactericidal deodorant 1% produce chicken manure biological organic fertilizer with biofermentation and common fermentation method;Specially
Benefit 201410399156.1 discloses a kind of chicken manure organic fertilizer of microbial fermentation, and raw material includes following components: chicken manure 45%~
70%;Chaff powder 5%~20%;Straw powder 15%~35%;EM stoste 0.1%~2%;Corn flour 0.1%~2%;Wheat bran 0.5%~4%;Specially
Benefit 201410603473.0 discloses a kind of preparation method of chicken manure fermenting organic fertilizer, and composition of raw materials is as follows: chicken manure 3000 is public
Jin, 400~600 kilograms of waste edible fungus slag charge, 100~200 kilograms of dregs of beans, 2~3 kilograms of duplex baking powder, yeast powder 20~30 are public
Jin;Patent 201610600091.1 discloses a kind of chicken manure fermenting organic fertilizer, is made of following raw material: chicken manure, straw powder, konjaku
Powder, corn flour, cellulase, zeolite, humic acid, potassium dihydrogen phosphate, urea, peat soil, leavening, soil conditioner, coating
Agent, potassium sulfate, borax, calcium superphosphate, above-mentioned raw materials carry out fermentation system to chicken manure by the combination of anaerobic fermentation and aerobic fermentation
Standby organic fertilizer;Patent 201710546184.5 discloses a kind of chicken manure organic fertilizer and preparation method thereof, raw material composition are as follows: chicken manure
30-40 parts, 10-20 parts of wheat bran, 10-20 parts of zeolite powder, 10-20 parts of needle mushroom slag, 5-10 parts of offal, 5-10 parts of sugarcane sugar residue,
1-5 parts of silkworm excrement, 1-5 parts of complex micro organism fungicide, complex micro organism fungicide is aspergillus oryzae, bacillus subtilis, saccharomycete, unwrapping wire
The mixture of bacterium, ammonifiers and photosynthetic bacteria.Although current microorganism compost fermentation poultry dung prepares the skill of chicken manure biological organic fertilizer
Art reaches its maturity, but that there are still production technologies is complicated, production cost is higher, Fertilizer Transformed is using horizontal lower in production technology at present
Etc. technologies drawback.
Summary of the invention
In order to overcome the above-mentioned deficiencies of the prior art, meet chicken manure harmless treatment and sustainable agriculture development to having
The demand of the chicken manure bio-organic fertilizer of efficient growth-promoting, volume increase, improvement soil and other effects, the present invention are provided at a kind of aerobic fermentation
The new process of chicken manure is managed, integrates modern mixed bacteria microorganism fermentation technique, developing efficient, green chicken manure fermenting biology has
Machine fertilizer, which is simple and efficient, deodorizing sterilizing effect is good, and production cost is low, is easy to industrial-scale production.
The present invention is achieved through the following technical solutions above-mentioned technical effect:
A kind of new process of aerobic fermentation processing chicken manure, it is characterised in that composite microbial bacteria is inoculated with after mixing chicken manure with auxiliary material
Then agent is made biological organic fertilizer by solid fermentation, screening, granulation, cooling dry, screening, technology of the package, specifically prepares work
Skill includes:
(1) mixing: inputting mixing machine for chicken manure, add auxiliary material, auxiliary material and chicken manure are uniformly mixed, and then passes through auger for material
It is delivered to fermentor;
(2) compound microorganism ferments: then the inoculating complex microorganism microbial inoculum into mixed material is aerated solid fermentation
3~5 days, wherein the complex micro organism fungicide includes bacillus coagulans, bacillus licheniformis, thermophilic heterotrophic ammonia-oxidizing bacterium
And aspergillus niger;
(3) it sieves: the material after fermentation is squeezed into screening machine, removal masonry tile fragment, plastics chemical fibre and bulky grain agglomeration;
(4) it pelletizes: material input granulator after screening is granulated;
(5) cooling drying: inputting drying machine for the material after granulation, dry to moisture content of material 15~18%;
(6) regrading: the material after drying is sieved, and removes powder;
(7) it packs: the material after regrading is packed, that is, be worth bio-organic fertilizer.
Auxiliary material in above-mentioned steps (1) is one or more of sawdust, rice bran, wheat bran, agricultural crop straw, mushroom residue
Mixture.
It is more than above-mentioned 100 mesh of auxiliary material smashing fineness.
It is controlled between material moisture 55~65% after chicken manure is mixed with auxiliary material in above-mentioned steps (1).
The inoculum concentration of above-mentioned steps (2) complex micro organism fungicide is the 0.2~0.3% of solid material mass ratio.
Bacillus coagulans in microbial bacterial agent in above-mentioned steps (2) in complex micro organism fungicide, bacillus licheniformis,
The amount ratio of thermophilic heterotrophic ammonia-oxidizing bacterium and aspergillus niger is 2~3:3~5:1~2:2~4.
Further, bacillus coagulans, lichens bud in the microbial bacterial agent in above-mentioned steps (2) complex micro organism fungicide
Spore bacillus, thermophilic heterotrophic ammonia-oxidizing bacterium and the optimal amount ratio of aspergillus niger are 2:5:2:3.
Above-mentioned bacillus coagulans, bacillus licheniformis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are the preparation method comprises the following steps: in nothing
Under the conditions of bacterium, is cultivated under the conditions of 28~32 DEG C using LB culture medium for 24 hours, liquid condensation bacillus, bacillus subtilis is made
Bacterium, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor, it is desirable that living bacteria count >=10 of seed liquor9cfu/mL。
Above-mentioned aspergillus niger seed liquor the preparation method comprises the following steps: aseptically, using potato culture medium in 35~40 DEG C of items
It is cultivated under part for 24 hours, aspergillus niger seed liquor is made, it is desirable that living bacteria count >=10 of seed liquor9cfu/mL。
It is carried out in Sealed Solid fermentor in above-mentioned solid fermentation process, fermentation ventilation quantity maintains heap body oxygen content to exist
12~16%;Deodorization device is equipped at the top of fermentor.
The present invention provides a kind of new process of aerobic fermentation processing chicken manure, gained fermenting organism organic fertilizer stink all disappears
It loses, quality is soft, not agglomerating, rich in nutritional ingredients such as probiotics, water soluble organic nitrogens in product, the content of organic matter in product >=
60%, content of microorganisms >=200,000,000/g;Product quality meets the regulation of NY525-2012, induced worm egg death rate and coli-group
Number index meets the requirement of NY884-2012.Results from pot experiment test shows that the application product has significant growth promotion, increases production, changes
The effects of kind soil environment is a kind of excellent novel bio-organic fertilizer.
The present invention provides a kind of new process of aerobic fermentation processing chicken manure, compared with prior art, has following significant excellent
Gesture:
(1) the application utilizes the direct aerobic fermentation chicken manure of complex microorganism, and fermentation time 3~5 days, it is decomposed to have broken traditional chicken manure
The longer technology drawback of fermentation time, improves resource utilization efficiency;
(2) the application directly carries out solid fermentation using complex micro organism fungicide and prepares biological organic fertilizer, makes full use of microorganism
Synergistic effect between strain, elimination restriction chicken manure fertilizer is big using existing stink, there are a large amount of pathogenic bacteria, heavy metals exceeding standard
Etc. drawbacks, the fertilizer efficiency of fermenting organism organic fertilizer is improved, to be obviously improved the fertile value of human excrement, in which: aspergillus niger
The ability of assimilation starch and cellulose is strong, and the structural carbohydrate of human excrement can be degraded to the available list of bacillus
The monosaccharide substance such as sugar, disaccharide, makes bacillus subtilis, bacillus coagulans etc. be able to good growth;Bacillus licheniformis
The pathogenic bacteria in human excrement can be killed during hot fermentation, and can improve the prebiotic bacterial content in bio-organic fertilizer, made
Bio-organic fertilizer is rich in a large amount of probiotics, can effectively adjust the balance of Soil Microorganism, organic matter, mine in decomposition soil
The organic or inorganics ingredient such as substance is eliminated soil hardening, desertification, salinization caused by relying on chemical fertilizer abuse throughout the year etc., is effectively improved
Soil improves the special efficacy of crop anti-adversity, improves crops production capacity;Releasing for soil with organic matter can especially be promoted
It puts, improves the physicochemical structure of salinization soil, increase soil agreegate and permeability, improve soil fertility;Thermophilic heterotrophism ammonia
The presence of oxidizing bacteria can prevent and treat the generation of ammonia, improve the organic nitrogen content in product, utilize chicken manure fertilizer
Zymotechnique more cleans, while may advantageously facilitate the growth of probiotics;Bacillus coagulans belong to facultative anaerobic bacteria, are having
It can be grown under oxygen and oxygen free condition, the application is overcome that may be present in solid-state fermentation tank by addition bacillus coagulans
The defect that microbial bacterial agent can not be grown in anaerobism subenvironment, and test result shows that the presence of bacillus coagulans can be shown
The pH value for reducing fermentation medium is write, promotes the growth of bacillus licheniformis, thermophilic heterotrophic ammonia-oxidizing bacterium, aspergillus niger etc. numerous
It educates;
(3) this application provides a kind of new processes of aerobic fermentation processing chicken manure, and using solid aerobic fermentation technique, energy will be effective
The pathogenic bacteria such as Shigella present in human excrement are eliminated, and can be by odorous substance NH3Transform into nitrate, H2S is changed into
H2SO4, other substances can be converted to CO2And H2O.Aspergillus niger and bacillus licheniformis can secrete a variety of enzymes and help fibre of degrading
Dimension element and poisonous and harmful substance, improve the rotten degree of human excrement, the growth-promoting for significantly improving product increases production effect;
(4) the application uses two step sieving technologies, and masonry tile fragment of not going out before solid fermentation process, plastics chemical fibre etc. are miscellaneous
Matter, but once sieved after fermentation, to remove above-mentioned impurity, the cost of removal of impurities is reduced, impurity-eliminating effect is improved;
(5) present invention uses solid fermentation, and technological condition for fermentation is mild, and low energy consumption, production cost is low, and the technical program is produced into
This is only to have reported the 1/3 of technical level at present, is played a significant role to promotion industrial economy benefit and large-scale promotion application;
And non-environmental-pollution, investment it is small, be easy to large-scale production.
Specific embodiment
The seed liquor of fermenting microbe in following embodiment is all made of following technique:
(1) bacillus coagulans, bacillus licheniformis, thermophilic heterotrophic ammonia-oxidizing bacterium seed liquor are the preparation method comprises the following steps: in sterile item
It under part, is cultivated under the conditions of 28~32 DEG C using LB culture medium for 24 hours, liquid condensation bacillus, bacillus subtilis, thermophilic is made
Hot heterotrophic ammonia-oxidizing bacterium seed liquor, it is desirable that living bacteria count >=10 of seed liquor9cfu/mL。
(2) aspergillus niger seed liquor the preparation method comprises the following steps: aseptically, using potato culture medium in 35~40 DEG C of conditions
For 24 hours, aspergillus niger seed liquor is made, it is desirable that living bacteria count >=10 of seed liquor in lower culture9cfu/mL。
Embodiment 1
A kind of new process of aerobic fermentation processing chicken manure, specific process step include:
(1) mixing: inputting mixing machine for chicken manure, add rice bran, rice bran and chicken manure are uniformly mixed, and control material moisture 55~
Between 65%, material is then delivered to by fermentor by auger;
(2) compound microorganism ferments: the inoculating complex microorganism microbial inoculum into mixed material, inoculum concentration are solid material quality
Then the 0.25% of ratio is aerated solid fermentation 3~5 days, wherein the complex micro organism fungicide include bacillus coagulans,
Bacillus licheniformis, thermophilic heterotrophic ammonia-oxidizing bacterium and aspergillus niger, wherein bacillus coagulans, bacillus licheniformis, thermophilic different
It supports ammonia oxidizing bacteria and the optimal amount ratio of aspergillus niger is 2:5:2:3;, fermentation ventilation quantity maintenance heap body oxygen content is 12~16%;
(3) it sieves: the material after fermentation is squeezed into screening machine, removal masonry tile fragment, plastics chemical fibre and bulky grain agglomeration;
(4) it pelletizes: material input granulator after screening is granulated;
(5) cooling drying: inputting drying machine for the material after granulation, dry to moisture content of material 15~18%;
(6) regrading: the material after drying is sieved, and removes powder;
(7) it packs: the material after regrading is packed, that is, be worth bio-organic fertilizer.
Embodiment 2
(1) mixing: inputting mixing machine for chicken manure, add wheat bran, wheat bran and chicken manure are uniformly mixed, and control material moisture 55~
Between 65%, material is then delivered to by fermentor by auger;
(2) compound microorganism ferments: the inoculating complex microorganism microbial inoculum into mixed material, inoculum concentration are solid material quality
Then the 0.2% of ratio is aerated solid fermentation 3~5 days, wherein the complex micro organism fungicide include bacillus coagulans,
Clothing bacillus, thermophilic heterotrophic ammonia-oxidizing bacterium and aspergillus niger, wherein bacillus coagulans, bacillus licheniformis, thermophilic heterotrophism
The amount ratio of ammonia oxidizing bacteria and aspergillus niger is 3:3:2:2;, fermentation ventilation quantity maintenance heap body oxygen content is 12~16%;
(3) it sieves: the material after fermentation is squeezed into screening machine, removal masonry tile fragment, plastics chemical fibre and bulky grain agglomeration;
(4) it pelletizes: material input granulator after screening is granulated;
(5) cooling drying: inputting drying machine for the material after granulation, dry to moisture content of material 15~18%;
(6) regrading: the material after drying is sieved, and removes powder;
(7) it packs: the material after regrading is packed, that is, be worth bio-organic fertilizer.
Embodiment 3
(1) mixing: chicken manure is inputted into mixing machine, agricultural crop straw is added, agricultural crop straw and chicken manure is uniformly mixed, controls object
Expect that material is then delivered to fermentor by auger between water content 55~65%;
(2) compound microorganism ferments: the inoculating complex microorganism microbial inoculum into mixed material, inoculum concentration are solid material quality
Then the 0.3% of ratio is aerated solid fermentation 3~5 days, wherein the complex micro organism fungicide include bacillus coagulans,
Clothing bacillus, thermophilic heterotrophic ammonia-oxidizing bacterium and aspergillus niger, wherein bacillus coagulans, bacillus licheniformis, thermophilic heterotrophism
The amount ratio of ammonia oxidizing bacteria and aspergillus niger is 2:5:1:4;, fermentation ventilation quantity maintenance heap body oxygen content is 12~16%;
(3) it sieves: the material after fermentation is squeezed into screening machine, removal masonry tile fragment, plastics chemical fibre and bulky grain agglomeration;
(4) it pelletizes: material input granulator after screening is granulated;
(5) cooling drying: inputting drying machine for the material after granulation, dry to moisture content of material 15~18%;
(6) regrading: the material after drying is sieved, and removes powder;
(7) it packs: the material after regrading is packed, that is, be worth bio-organic fertilizer.
Embodiment 4 compares influence of the different fermentations technique to bio-organic fertilizer index
1, index determining method
Content of cellulose: using acid detergent method (ADF), weighs the air-dry sample 1.00g sieved by 1mm, is added with 16
The acid detergent that the sulfuric acid of alkyl trimethyl ammonium bromide (CTAB) and 0.50mol/L are prepared, heating are condensed back after boiling
60min, then sample is depressurized with glass pot formula filter and is filtered, residue is stirred after dissipating and use hot water injection, and instills acetone promotion
Colloidal substance dissolution drains acetone after filter is net, is put into 3h in 100 DEG C of air dry ovens, and cooling weighing obtains crude fibre after calculating
Percentage composition.
Content of humic acid: weighing 20 mesh sample 0.2g, and the 1L alkali configured with 15g sodium pyrophosphate and 7g sodium hydroxide is added
Property leaching liquor 70mL, boiling water bath heats 30min, takes after constant volume 5mL that 0.8N potassium bichromate 5mL and the 15mL concentrated sulfuric acid is added, then boils
After heating water bath 30min, instills Phen indicator 3 and drip, becoming brick-red from yellow green with ferrous sulfate titration solution is
Titration end-point records ferrous sulfate dosage, and replaces sample to do blank test with above-mentioned steps with quartz sand.
The measurement of germination index: fresh sample mixes (W/V) with deionized water with 1:10, after being placed in horizontal shaker concussion 2h
Filtering.It takes 5mL filtrate to be added to be covered in the culture dish of filter paper, 20 Lepidium apetalum seeds, blank control is placed in each culture dish
For deionized water.Culture dish is placed in 25 DEG C of constant incubators after dark culture 3 days, measures chitting piece number and root long, often
A sample setting repeats three times.Chitting piece number=sample germination percentage (%) × sample root long × 100%/control germination percentage (%) × sample
Product root long.
2, test grouping
Test is divided into embodiment 1-3 test group, (redistilled water replaces composite bacteria agent seed liquor to blank control group, remaining technique is the same as real
Apply example 1), control 1 group (complex micro organism fungicide contains only bacillus licheniformis, and the zymotechniques such as inoculum concentration are with embodiment 1), control
2 groups (complex micro organism fungicide contains only thermophilic heterotrophic ammonia-oxidizing bacterium, and the zymotechniques such as inoculum concentration are with embodiment 1), 3 groups of control
(complex micro organism fungicide contains only aspergillus niger, and the zymotechniques such as inoculum concentration are with embodiment 1), (complex micro organism fungicide is only for 4 groups of control
Containing bacillus coagulans, the zymotechniques such as inoculum concentration are with embodiment 1).
3, data analysis statistical
The random single factor analysis of data application SPSS11.5 software, and LSD multiple comparison graph is used, with " average value ± standard
Poor (SD) " is indicated.
4, test result
Influence of 1 different technology conditions of table to bio-organic fertilizer index
Testing index |
Crude fibre (%) |
Humic acid (%) |
Germination index (%) |
Blank control group |
40.35±1.54 |
11.36±1.23 |
21.68±1.92 |
1 test group of embodiment |
12.04±0.91 |
31.29±1.06 |
88.23±1.73 |
2 test group of embodiment |
15.32±1.57 |
29.69±1.52 |
82.48±1.95 |
3 test group of embodiment |
14.86±2.03 |
30.91±2.17 |
83.73±2.07 |
Compare 1 group |
36.37±1.97 |
14.48±1.06 |
41.29±1.53 |
Compare 2 groups |
38.89±1.04 |
15.31±1.39 |
30.84±1.28 |
Compare 3 groups |
28.45±1.42 |
17.02±1.05 |
37.21±1.62 |
Compare 4 groups |
39.29±1.15 |
14.29±1.01 |
33.28±0.99 |
Above-mentioned test result shows that the synergistic effect between composite microbial fermentation bacteria group has been obviously improved the growth promoting volume increase effect of product
Fruit, the composition for changing fermentative microflora can significantly reduce product rotten index.
The above examples are only used to illustrate the technical scheme of the present invention, rather than is limited;Although referring to aforementioned reality
It applies example to be described in detail to by invention, but for those of ordinary skill in the art, it still can be to aforementioned reality
Technical solution documented by example is applied to modify or equivalent replacement of some of the technical features;And to these modifications
Or replacement, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.