CN104232551A - Pig-chicken excrement fermenting compound bacteria, preparation method and method of utilizing compound bacteria to ferment and prepare organic fertilizer - Google Patents
Pig-chicken excrement fermenting compound bacteria, preparation method and method of utilizing compound bacteria to ferment and prepare organic fertilizer Download PDFInfo
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Abstract
The invention discloses pig-chicken excrement fermenting compound bacteria, a preparation method and a method of utilizing the compound bacteria to ferment and prepare an organic fertilizer. The fermenting compound bacteria comprise the following raw material bacteria: bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus megatherium and trichoderma viride. The method for preparing the organic fertilizer comprises the following steps: mixing pig-chicken excrement with crushed straws, adding the fermenting compound bacteria to the mixture according to a dosage of 1-2 Kg per ton, mixing uniformly, the moisture being 50-65 wt%, stacking the mixed raw materials, keeping the temperature at 55-65 DEG C to conduct fermentation, two days after the fermentation, turning the stacked materials for the first time, and then turning the materials once every one or two days, keeping the moisture at 50-65 wt% during the fermentation phase, and after fermentation, obtaining the organic fertilizer with a moisture content of 20-30 wt%. The pig-chicken excrement fermenting compound bacteria disclosed by the invention can be used for fermenting the pig and chicken excrement; compared to the prior art, the method for preparing the organic fertilizer is low in productive investment and production cost, convenient to use, free of odor, and good in the fermentation and decomposing effect.
Description
Technical field
The present invention relates to technical field of microbial fermentation, be specifically related to a kind of for pig, chicken manure just fermentation for the composite bacteria of fertilizer and fermentation technique thereof.
Background technology
Pig (chicken) excrement should be a kind of well farm manure, is foster and apply fertilizer to improve the high-quality organic manure resource of soil.If but directly used fresh pig (chicken) ight soil, crop will be produced and hinder root, burn the negative impacts such as seedling, disease, crop smothering.If by existing natural pile fermentation process, there is following several respects drawback: one is stack retting time long (4-6 months), weak effect; Two is bank up for a long time both to account for place, pollutes again to surrounding environment; Three be nature pile fermentation ight soil is rotten wet shape, huge stench, is difficult to transport, is also difficult to directly use.
And with microbe fermentation method process pig (chicken) excrement, fermenting process can not only be accelerated, shorten fermentation time, avoid waste, improve the nutritive value of pig (chicken) excrement; And by the temperature cicken feces dried that fermentation produces, save energy consumption, and with low cost, simple to operate, be easy to promote, thus more and more obtain the attention of people.At present, the problems such as although there is relevant fermenting agent to come out, all there is ferment effect difference, fermentation time is long, and production cost is high, the fertilizer efficiency difference after fermentation.
Summary of the invention
First technical problem to be solved by this invention is: the deficiency existed for prior art, provide that a kind of production cost is low, ferment effect good, the fertilizer fertilizer efficiency that obtains of fermenting be high, the pig to farm crop fanout free region, chicken manure just fermenting compound fungus.
Second technical problem to be solved by this invention is: the deficiency existed for prior art, provides that a kind of production cost is low, ferment effect good, the fertilizer fertilizer efficiency that obtains of fermenting is high, preparation method to the pig of farm crop fanout free region, chicken manure just fermenting compound fungus.
3rd technical problem to be solved by this invention is: the deficiency existed for prior art, a kind of method using fermenting compound fungus fermented-pig, chicken manure just to prepare fertilizer is provided, the method investment of production is little, production cost is low, ferment effect is good, the fertilizer fertilizer efficiency that obtains of fermenting is high, to farm crop fanout free region.
For solving above-mentioned first technical problem, technical scheme of the present invention is:
One boar, chicken manure just fermenting compound fungus, comprises the raw material bacterial classification of following parts by weight:
For solving above-mentioned second technical problem, technical scheme of the present invention is:
Preparation pig, the method of chicken manure just fermenting compound fungus, comprise the following steps: respectively by subtilis, Bacillus licheniformis, Bacillus coagulans, the bacterial classification of bacillus megaterium and viride carries out high-density culture, first being seeded to and volume ratio is housed is activation culture in the shaking flask of the substratum of 15 ~ 30%, fermentation enlarged culturing is carried out by cultured strain inoculation to fermentor tank, various single bacterium enlarged culturing obtained dehydrates, be prepared into hypopus microbial dry powder, then according to subtilis 20 ~ 30 parts, Bacillus licheniformis 20 ~ 25 parts, Bacillus coagulans 10 ~ 25 parts, the weight ratio of bacillus megaterium 10 ~ 20 parts and viride 8 ~ 15 parts, be mixed to get described fermenting compound fungus.
Wherein, the preparation of the hypopus microbial dry powder of described subtilis comprises the following steps:
Bacillus subtilis strain on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 15 ~ 30%, the formula of described activation medium is glucose 18 ~ 22g/L, peptone 12 ~ 18g/L, sodium-chlor 3 ~ 7g/L and extractum carnis 0.2 ~ 0.8g/L, be 36 ~ 40 DEG C in culture temperature, shaking flask rotating speed 180 ~ 220rpm cultivates 20 ~ 32h;
The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, volume inoculum size is 1 ~ 3%, the formula of described fermention medium is Semen Maydis powder 10 ~ 15g/L, glucose 3 ~ 8g/L, soybean cake powder 18 ~ 22g/L, fish meal 3 ~ 8g/L, calcium carbonate 5 ~ 9g/L, ammonium sulfate 0.8 ~ 1.2g/L, dipotassium hydrogen phosphate 0.1 ~ 0.5g/L, magnesium sulfate 0.1 ~ 0.3g/L and manganous sulfate 0.1 ~ 0.3g/L, it is 36 ~ 40 DEG C in culture temperature, fermentor tank mixing speed 200 ~ 220rpm cultivates 20 ~ 32h, bacillus subtilis bacterial content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the subtilis then obtained fermenting is prepared into hypopus microbial dry powder.
Wherein, the preparation of the hypopus microbial dry powder of described Bacillus licheniformis comprises the following steps:
Bacillus licheniformis strain on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 15 ~ 30%, the formula of described activation medium is peptone 8 ~ 12g/L, extractum carnis 1 ~ 5g/L and sodium-chlor 3 ~ 7g/L, be 36 ~ 40 DEG C in culture temperature, shaking flask rotating speed 180 ~ 220rpm cultivates 20 ~ 32h;
The Bacillus licheniformis strain that activation culture is good is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, volume inoculum size is 1 ~ 3%, the formula of described fermention medium is soybean cake powder 13 ~ 17g/L, Semen Maydis powder 13 ~ 17g/L, dipotassium hydrogen phosphate 0.05 ~ 0.15g/L, potassium primary phosphate 0.05 ~ 0.15g/L and calcium chloride 2 ~ 6g/L, it is 36 ~ 40 DEG C in culture temperature, fermentor tank mixing speed 200 ~ 220rpm cultivates 20 ~ 32h, Bacillus licheniformis content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the Bacillus licheniformis then obtained fermenting is prepared into hypopus microbial dry powder.
Wherein, the preparation of the hypopus microbial dry powder of described Bacillus coagulans comprises the following steps:
Bacillus coagulans strain inoculation on the slant medium cultivate purifying is in the shaking flask of the activation medium of 15 ~ 30% to being equipped with volume ratio, the formula of described activation medium is glucose 10 ~ 12g/L, peptone 8 ~ 12g/L, extractum carnis 1 ~ 5g/L and sodium-chlor 3 ~ 7g/L, be 36 ~ 40 DEG C in culture temperature, shaking flask rotating speed 180 ~ 220rpm cultivates 20 ~ 32h;
By Bacillus coagulans strain inoculation good for activation culture to being equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, volume inoculum size is 1 ~ 3%, the formula of described fermention medium is wheat bran 20 ~ 25g/L, glucose 11 ~ 15g/L, soybean cake powder 12 ~ 18g/L, dipotassium hydrogen phosphate 0.05 ~ 0.15g/L, magnesium sulfate 0.05 ~ 0.15g/L and calcium chloride 2 ~ 6g/L, it is 36 ~ 40 DEG C in culture temperature, fermentor tank mixing speed 200 ~ 220rpm cultivates 30 ~ 40h, Bacillus coagulans content>=10 in fermentor tank
9individual/ml, is then prepared into hypopus microbial dry powder by the Bacillus coagulans list bacterium drying obtained of fermenting.
Wherein, the preparation of the hypopus microbial dry powder of described bacillus megaterium comprises the following steps:
Bacillus megaterium strain inoculation on the slant medium cultivate purifying is in the shaking flask of the activation medium of 15 ~ 30% to being equipped with volume ratio, the formula of described activation medium is peptone 8 ~ 12g/L, extractum carnis 1 ~ 5g/L and sodium-chlor 3 ~ 7g/L, be 36 ~ 40 DEG C in culture temperature, shaking flask rotating speed 180 ~ 220rpm cultivates 20 ~ 32h;
By bacillus megaterium strain inoculation good for activation culture to being equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, volume inoculum size is 1 ~ 3%, the formula of described fermention medium is soybean cake powder 20 ~ 26g/L, Semen Maydis powder 12 ~ 20g/L, dipotassium hydrogen phosphate 0.05 ~ 0.15g/L, potassium primary phosphate 0.05 ~ 0.15g/L and calcium chloride 2 ~ 6g/L, it is 36 ~ 40 DEG C in culture temperature, fermentor tank mixing speed 200 ~ 220rpm cultivates 20 ~ 32h, bacillus megaterium content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the bacillus megaterium then obtained fermenting is prepared into hypopus microbial dry powder.
Wherein, the preparation of the hypopus microbial dry powder of described viride comprises the following steps:
Viride bacterial classification on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 15 ~ 30%, described activation medium is wort potato sucrose substratum, be 28 ~ 30 DEG C, shaking flask rotating speed 140 ~ 180rpm, activation culture 32 ~ 48h in culture temperature;
Viride bacterial classification good for activation culture is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, inoculum size is volume ratio 1 ~ 3%, the formula of described fermention medium is glucose 8 ~ 12g/L, corn cob 5 ~ 6g/L, calcium chloride 0.2 ~ 0.6g/L, magnesium sulfate 0.1 ~ 0.5g/L, potassium primary phosphate 18 ~ 22g/L, yeast extract paste 8 ~ 12g/L, ferrous sulfate 3 ~ 7mg/L, zinc sulfate 1.0 ~ 1.8mg/L, cobalt chloride 3.5 ~ 4.0mg/L and manganous sulfate 1.2 ~ 1.8mg/L, it is 28 ~ 30 DEG C in culture temperature, fermentor tank mixing speed 140 ~ 180rpm, enlarged culturing 68 ~ 84h, to mould content>=10 of fermentor tank Green wood
8individual/ml, is then prepared into hypopus microbial dry powder by the viride list bacterium obtained of fermenting through lyophilize.
Subtilis in the present invention is the important production bacterium of α-amylase and neutral protease, the subtilyne produced in subtilis thalli growth process, polymyxin, nystatin, linear gramicidins isoreactivity material, have obvious restraining effect to the conditioned pathogen of pathogenic bacterium or autogenous infection; The pathogenic bacterium in ight soil can be suppressed; Enzyme such as subtilis thalline self synthesis α-amylase, proteolytic enzyme etc., can decompose the macrobead such as starch, protein in pig (chicken) excrement; Subtilis has very strong restraining effect to unwanted bacterias such as the intestinal bacteria in pig (chicken) ight soil and baculoviruss.
Bacillus licheniformis in the present invention has stronger proteolytic enzyme, lipase, diastatic activity, can decompose starch, protein and lipid in pig (chicken) excrement.This bacterium can also produce activity resistent material, can suppress the growth and breeding of pathogenic bacterium; Plurality of plant diseases can also be prevented and treated.
Bacillus coagulans in the present invention can produce some acidic substance in growth and breeding process, suppresses the growth of pathogenic bacterium in pig (chicken) excrement, and all right activating soil, for plant provides nutrition.
Bacillus megaterium in the present invention by small-molecule substances such as macrobead breaks down into amino acids such as the protein in pig (chicken) excrement, can also can be used to the solid potash fertilizer of production phosphorus decomposing.Bacillus megaterium has effect of organophosphorus in soil of well degrading, it is the conventional bacterial classification producing biological organic fertilizer, bacillus megaterium can secrete calcium phosphorous compound, ferro-phosphorus and the aluminium phosphorus compound that in the substance dissolves soil such as organic acid, crop not easily absorbs, impel dissolving and the utilization of soil inavailable phosphorus, and then assist the growth of Soil Microorganism, prevention soil disease occurs, and reduces the problems such as continuous cropping obstacle, to reach effect of soil improvement.
Viride in the present invention can produce multiplely has bioactive enzyme system, as: cellulase, chitinase, zytase etc.Viride is one of cellulase-producing the highest active bacterial strain; the cellulase produced can be degraded the fiber in pig (chicken) excrement or in filler; effect is very good; it is again a kind of resourceful antagonistic microbe; there is important effect in biological control; there is protection and treatment double effects, effectively can prevent and treat soil-borne disease.
For solving above-mentioned 3rd technical problem, technical scheme of the present invention is:
Use fermenting compound fungus fermented-pig, chicken manure just prepare the method for fertilizer, comprise the following steps:
(1) swine excrement or chicken manure are just mixed with chippy stalk, added to mix by described fermenting compound fungus obtain mixing raw material according to the consumption of 1 ~ 2Kg/t, the moisture of mixing raw material is 50 ~ 65wt%;
(2) the mixing raw material code of step (1) heap is in strip fertile heap, holding temperature is 55 ~ 65 DEG C and starts fermentation, fermentation carries out first time turning two days later, carries out a turning, keep moisture content 50 ~ 65wt% between yeast phase after secondary every 1 ~ 2 day;
(3), after reducing to normal temperature to the fermentation heap temperature of step (2), fermentation ends, obtains the fertilizer that water ratio is 20 ~ 30wt%.
Improve as one, between yeast phase, bar shaped fertilizer heap arranges the ventilation hole that multiple diameter is 1.5 ~ 2cm.
Preferred as one, in described mixing raw material, ight soil is 3 ~ 5:1 ~ 2 with the mixed weight ratio of stalk.
Owing to have employed technique scheme, the invention has the beneficial effects as follows:
Pig of the present invention, chicken manure is fermenting compound fungus just, comprises the raw material bacterial classification of following parts by weight: subtilis 20 ~ 30 parts, Bacillus licheniformis 20 ~ 25 parts, Bacillus coagulans 10 ~ 25 parts, bacillus megaterium 10 ~ 20 parts, viride 8 ~ 15 parts, by subtilis, Bacillus licheniformis, Bacillus coagulans, the bacterial classification of bacillus megaterium and viride adopts rational proportion mixing, obtains pig, chicken manure is fermenting compound fungus just, prove do not have Antagonism each other, and mutually work in coordination with between each bacterial classification, the amylase activity of bacillus subtilis secretion is the highest after each bacterial classification is composite through antimicrobial experiment, is secondly proteolytic enzyme, lipase, and the protease activity of Bacillus licheniformis secretion is the highest, the cellulase activity of viride secretion is the highest, is secondly zytase, chitinase, the enzyme system that these three kinds of bacterium produce can by pig (chicken) ight soil not by the protein of animal use, starch, fat, Mierocrystalline cellulose thoroughly decomposes, and changes into the small-molecule substance that plant easily absorbs.Bacillus coagulans and bacillus megaterium then can produce various acidic substance, suppress the growth of pathogenic bacterium in pig (chicken) excrement, and bacillus megaterium also has the effect of P and K decomposing, all right activating soil, for plant provides nutrition can by macromolecular substance such as the protein in ight soil, starch, fibers, the small-molecule substance that fast degradation becomes plant easily to absorb, pig (chicken) ight soil, by the mutual synergy between these bacterial classifications, finally becomes and is rich in organic and beneficial microorganism biological organic fertilizer.And in fermentation maturity process, fermentation materials heats up very fast, and temperature can reach 60 ~ 70 degree fast, effectively can kill the objectionable impurities such as worm's ovum, grass-seed in ight soil, fermentation period is short, and general about 7 days of summer can ferment, and about 20 days winter completed, ight soil odorless after fermentation, water ratio is below 30wt%, and N P and K total nutrient content reaches more than 55g/ ㎏, and organic content is more than 40%, colititre cannot detect, and parasitic ovum mortality ratio is more than 98%.
The present invention is used for pig, chicken manure fermentation, and compared to existing technology, investment of production is little, and production cost is low, easy to use, odorless, and fermentation maturity is effective.
Embodiment
The present invention is set forth further below in conjunction with specific embodiment.
Embodiment 1
One boar, chicken manure just fermenting compound fungus, comprises the raw material bacterial classification of following parts by weight:
Embodiment 2
One boar, chicken manure just fermenting compound fungus, comprises the raw material bacterial classification of following parts by weight:
Embodiment 3
One boar, chicken manure just fermenting compound fungus, comprises the raw material bacterial classification of following parts by weight:
Embodiment 4
Bacillus subtilis strain on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 20%, the formula of described activation medium is glucose 20g/L, peptone 13g/L, sodium-chlor 4g/L and extractum carnis 0.7g/L, be 38 DEG C in culture temperature, shaking flask rotating speed 200rpm cultivates 24h;
The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 55%, volume inoculum size is 1.5%, the formula of described fermention medium is Semen Maydis powder 12g/L, glucose 4g/L, soybean cake powder 22g/L, fish meal 4g/L, calcium carbonate 6g/L, ammonium sulfate 1.2g/L, dipotassium hydrogen phosphate 0.3g/L, magnesium sulfate 0.15g/L and manganous sulfate 0.1g/L, it is 38 DEG C in culture temperature, fermentor tank mixing speed 200rpm cultivates 24h, bacillus subtilis bacterial content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the subtilis then obtained fermenting is prepared into hypopus microbial dry powder.
Bacillus licheniformis strain on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 18%, the formula of described activation medium is peptone 9g/L, extractum carnis 4g/L and sodium-chlor 7g/L, be 38 DEG C in culture temperature, shaking flask rotating speed 200rpm cultivates 24h;
The Bacillus licheniformis strain that activation culture is good is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 60%, volume inoculum size is 2%, the formula of described fermention medium is soybean cake powder 13g/L, Semen Maydis powder 17g/L, dipotassium hydrogen phosphate 0.10g/L, potassium primary phosphate 0.10g/L and calcium chloride 3g/L, it is 38 DEG C in culture temperature, fermentor tank mixing speed 220rpm cultivates 24h, Bacillus licheniformis content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the Bacillus licheniformis then obtained fermenting is prepared into hypopus microbial dry powder.
Bacillus coagulans strain inoculation on the slant medium cultivate purifying is in the shaking flask of the activation medium of 15% to being equipped with volume ratio, the formula of described activation medium is glucose 12g/L, peptone 8g/L, extractum carnis 2g/L and sodium-chlor 4g/L, be 38 DEG C in culture temperature, shaking flask rotating speed 190rpm cultivates 24h;
By Bacillus coagulans strain inoculation good for activation culture to being equipped with in fermentor tank that volume ratio is the fermention medium of 55%, volume inoculum size is 1%, the formula of described fermention medium is wheat bran 23g/L, glucose 13g/L, soybean cake powder 14g/L, dipotassium hydrogen phosphate 0.15g/L, magnesium sulfate 0.15g/L and calcium chloride 2g/L, it is 38 DEG C in culture temperature, fermentor tank mixing speed 220rpm cultivates 38h, Bacillus coagulans content>=10 in fermentor tank
9individual/ml, is then prepared into hypopus microbial dry powder by the Bacillus coagulans list bacterium drying obtained of fermenting.
Bacillus megaterium strain inoculation on the slant medium cultivate purifying is in the shaking flask of the activation medium of 30% to being equipped with volume ratio, the formula of described activation medium is peptone 12g/L, extractum carnis 1g/L and sodium-chlor 3g/L, be 38 DEG C in culture temperature, shaking flask rotating speed 220rpm cultivates 26h;
By bacillus megaterium strain inoculation good for activation culture to being equipped with in fermentor tank that volume ratio is the fermention medium of 60%, volume inoculum size is 1.5%, the formula of described fermention medium is soybean cake powder 25g/L, Semen Maydis powder 15g/L, dipotassium hydrogen phosphate 0.05g/L, potassium primary phosphate 0.15g/L and calcium chloride 4g/L, it is 38 DEG C in culture temperature, fermentor tank mixing speed 200rpm cultivates 28h, bacillus megaterium content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the bacillus megaterium then obtained fermenting is prepared into hypopus microbial dry powder.
Viride bacterial classification on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 30%, described activation medium is wort potato sucrose substratum, be 30 DEG C, shaking flask rotating speed 160rpm, activation culture 36h in culture temperature;
Viride bacterial classification good for activation culture is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 70%, inoculum size is volume ratio 1.5%, the formula of described fermention medium is glucose 10g/L, corn cob 5.5g/L, calcium chloride 0.4g/L, magnesium sulfate 0.3g/L, potassium primary phosphate 18g/L, yeast extract paste 10g/L, ferrous sulfate 4mg/L, zinc sulfate 1.3mg/L, cobalt chloride 3.5mg/L and manganous sulfate 1.5mg/L, it is 28 DEG C in culture temperature, fermentor tank mixing speed 180rpm, enlarged culturing 68h, to mould content>=10 of fermentor tank Green wood
8individual/ml, is then prepared into hypopus microbial dry powder by the viride list bacterium obtained of fermenting through lyophilize.
Respectively by prepare subtilis, Bacillus licheniformis, Bacillus coagulans, bacillus megaterium and viride hypopus microbial dry powder, according to subtilis 24 parts, Bacillus licheniformis 24 parts, Bacillus coagulans 15 parts, the weight ratio of bacillus megaterium 18 parts and viride 10 parts, is mixed to get described fermenting compound fungus.
Embodiment 5
Bacillus subtilis strain on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 25%, the formula of described activation medium is glucose 20g/L, peptone 14g/L, sodium-chlor 5g/L and extractum carnis 0.3g/L, be 38 DEG C in culture temperature, shaking flask rotating speed 220rpm cultivates 30h;
The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 60%, volume inoculum size is 2.5%, the formula of described fermention medium is Semen Maydis powder 15g/L, glucose 6g/L, soybean cake powder 21g/L, fish meal 5g/L, calcium carbonate 6g/L, ammonium sulfate 0.8g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.3g/L and manganous sulfate 0.2g/L, it is 38 DEG C in culture temperature, fermentor tank mixing speed 200rpm cultivates 30h, bacillus subtilis bacterial content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the subtilis then obtained fermenting is prepared into hypopus microbial dry powder.
Bacillus licheniformis strain on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 25%, the formula of described activation medium is peptone 10g/L, extractum carnis 3g/L and sodium-chlor 5g/L, be 38 DEG C in culture temperature, shaking flask rotating speed 200rpm cultivates 28h;
The Bacillus licheniformis strain that activation culture is good is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 60%, volume inoculum size is 1.5%, the formula of described fermention medium is soybean cake powder 15g/L, Semen Maydis powder 15g/L, dipotassium hydrogen phosphate 0.1g/L, potassium primary phosphate 0.1g/L and calcium chloride 4g/L, it is 38 DEG C in culture temperature, fermentor tank mixing speed 200rpm cultivates 28h, Bacillus licheniformis content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the Bacillus licheniformis then obtained fermenting is prepared into hypopus microbial dry powder.
Bacillus coagulans strain inoculation on the slant medium cultivate purifying is in the shaking flask of the activation medium of 20% to being equipped with volume ratio, the formula of described activation medium is glucose 11g/L, peptone 10g/L, extractum carnis 3g/L and sodium-chlor 5g/L, be 38 DEG C in culture temperature, shaking flask rotating speed 200rpm cultivates 28h;
By Bacillus coagulans strain inoculation good for activation culture to being equipped with in fermentor tank that volume ratio is the fermention medium of 60%, volume inoculum size is 1.5%, the formula of described fermention medium is wheat bran 22g/L, glucose 13g/L, soybean cake powder 15g/L, dipotassium hydrogen phosphate 0.1g/L, magnesium sulfate 0.1g/L and calcium chloride 4g/L, it is 38 DEG C in culture temperature, fermentor tank mixing speed 220rpm cultivates 35h, Bacillus coagulans content>=10 in fermentor tank
9individual/ml, is then prepared into hypopus microbial dry powder by the Bacillus coagulans list bacterium drying obtained of fermenting.
Bacillus megaterium strain inoculation on the slant medium cultivate purifying is in the shaking flask of the activation medium of 25% to being equipped with volume ratio, the formula of described activation medium is peptone 10g/L, extractum carnis 3g/L and sodium-chlor 4g/L, be 38 DEG C in culture temperature, shaking flask rotating speed 200rpm cultivates 28h;
By bacillus megaterium strain inoculation good for activation culture to being equipped with in fermentor tank that volume ratio is the fermention medium of 60%, volume inoculum size is 2%, the formula of described fermention medium is soybean cake powder 25g/L, Semen Maydis powder 15g/L, dipotassium hydrogen phosphate 0.15g/L, potassium primary phosphate 0.15g/L and calcium chloride 3g/L, it is 38 DEG C in culture temperature, fermentor tank mixing speed 210rpm cultivates 28h, bacillus megaterium content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the bacillus megaterium then obtained fermenting is prepared into hypopus microbial dry powder.
Viride bacterial classification on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 20%, described activation medium is wort potato sucrose substratum, be 28 DEG C, shaking flask rotating speed 180rpm, activation culture 48h in culture temperature;
Viride bacterial classification good for activation culture is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 60%, inoculum size is volume ratio 2%, the formula of described fermention medium is glucose 10g/L, corn cob 6g/L, calcium chloride 0.2g/L, magnesium sulfate 0.3g/L, potassium primary phosphate 20g/L, yeast extract paste 10g/L, ferrous sulfate 6mg/L, zinc sulfate 1.0mg/L, cobalt chloride 4.0mg/L and manganous sulfate 1.2mg/L, it is 30 DEG C in culture temperature, fermentor tank mixing speed 160rpm, enlarged culturing 80h, to mould content>=10 of fermentor tank Green wood
8individual/ml, is then prepared into hypopus microbial dry powder by the viride list bacterium obtained of fermenting through lyophilize.
Respectively by prepare subtilis, Bacillus licheniformis, Bacillus coagulans, bacillus megaterium and viride hypopus microbial dry powder, according to subtilis 28 parts, Bacillus licheniformis 22 parts, Bacillus coagulans 18 parts, the weight ratio of bacillus megaterium 16 parts and viride 12 parts, is mixed to get described fermenting compound fungus.
Embodiment 6
Respectively by subtilis, Bacillus licheniformis, Bacillus coagulans, the bacterial classification of bacillus megaterium and viride carries out high-density culture, first being seeded to and volume ratio is housed is activation culture in the shaking flask of the substratum of 25%, fermentation enlarged culturing is carried out by cultured strain inoculation to fermentor tank, various single bacterium enlarged culturing obtained dehydrates, be prepared into hypopus microbial dry powder, then according to subtilis 24 parts, Bacillus licheniformis 23 parts, Bacillus coagulans 19 parts, the weight ratio of bacillus megaterium 16 parts and viride 12 parts, be mixed to get described fermenting compound fungus.
Embodiment 7
(1) pig manure is mixed according to the ratio that weight ratio is 5:2 with chippy stalk, the fermenting compound fungus of embodiment 2 is added to mix according to the consumption of 1Kg/t and obtains mixing raw material, the moisture of mixing raw material is 55wt%, mixed fermentation raw material hand is held can be agglomerating, spread the centre of the palm out and rock the group that can fall apart, show that moisture content is suitable;
(2) the mixing raw material code of step (1) heap is in strip fertile heap, holding temperature is 60 ~ 65 DEG C and starts fermentation, fermentation carries out first time turning two days later, first after turning, temperature continued to rise at following one day, after maintaining thermophilic fermentation 1 day again, carry out the second time turning of fermentation stage, a turning was carried out every 1 ~ 2 day after secondary, between yeast phase, with waddy, the ventilation hole that multiple diameter is 1.5 ~ 2cm is set on bar shaped fertilizer heap, moisture content 50 ~ 55wt% is kept between yeast phase, if moisture content is high, add appropriate chippy stalk to reduce moisture,
(3), after reducing to normal temperature to the fermentation heap temperature of step (2), fermentation ends, obtains the fertilizer that water ratio is 26wt%.The pig manure become thoroughly decomposed, its color is brown, is substantially destitute of smell, and temperature of charge is close to temperature, and shatter value is good; N P and K total nutrient content reaches 55g/ ㎏, pH 7.6, and organic content is 40%, and colititre cannot detect, and parasitic ovum mortality ratio is 98%.
Embodiment 8
(1) chicken manure is mixed according to the ratio that weight ratio is 3:1 with chippy stalk, fermenting compound fungus embodiment 5 prepared adds to mix according to the consumption of 1.5Kg/t and obtains mixing raw material, the moisture of mixing raw material is 53wt%, mixed fermentation raw material hand is held can be agglomerating, spread the centre of the palm out and rock the group that can fall apart, show that moisture content is suitable;
(2) the mixing raw material code of step (1) heap is in strip fertile heap, holding temperature is 62 ~ 65 DEG C and starts fermentation, fermentation carries out first time turning two days later, first after turning, temperature continued to rise at following one day, after maintaining thermophilic fermentation 1 day again, carry out the second time turning of fermentation stage, a turning was carried out every 1 ~ 2 day after secondary, between yeast phase, with waddy, the ventilation hole that multiple diameter is 1.5 ~ 2cm is set on bar shaped fertilizer heap, moisture content 50 ~ 52wt% is kept between yeast phase, if moisture content is high, add appropriate chippy stalk to reduce moisture,
(3), after reducing to normal temperature to the fermentation heap temperature of step (2), fermentation ends, obtains the fertilizer that water ratio is 25wt%.The chicken manure become thoroughly decomposed, its color is chocolate, slightly fragrance or Earthy Taste, and temperature of charge is close to temperature, and shatter value is good; N P and K total nutrient content reaches 60g/ ㎏, pH 7.5, and organic content is 43%, and colititre cannot detect, and parasitic ovum mortality ratio is 98.5%.
Claims (10)
1. a boar, chicken manure just fermenting compound fungus, is characterized in that the raw material bacterial classification comprising following parts by weight:
2. prepare pig as claimed in claim 1, the method of chicken manure just fermenting compound fungus, it is characterized in that comprising the following steps: respectively by subtilis, Bacillus licheniformis, Bacillus coagulans, the bacterial classification of bacillus megaterium and viride carries out high-density culture, first being seeded to and volume ratio is housed is activation culture in the shaking flask of the substratum of 15 ~ 30%, fermentation enlarged culturing is carried out by cultured strain inoculation to fermentor tank, various single bacterium enlarged culturing obtained dehydrates, be prepared into hypopus microbial dry powder, then according to subtilis 20 ~ 30 parts, Bacillus licheniformis 20 ~ 25 parts, Bacillus coagulans 10 ~ 25 parts, the weight ratio of bacillus megaterium 10 ~ 20 parts and viride 8 ~ 15 parts, be mixed to get described fermenting compound fungus.
3. the preparation method of pig as claimed in claim 1, chicken manure just fermenting compound fungus, is characterized in that the preparation of the hypopus microbial dry powder of described subtilis comprises the following steps:
Bacillus subtilis strain on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 15 ~ 30%, the formula of described activation medium is glucose 18 ~ 22g/L, peptone 12 ~ 18g/L, sodium-chlor 3 ~ 7g/L and extractum carnis 0.2 ~ 0.8g/L, be 36 ~ 40 DEG C in culture temperature, shaking flask rotating speed 180 ~ 220rpm cultivates 20 ~ 32h;
The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, volume inoculum size is 1 ~ 3%, the formula of described fermention medium is Semen Maydis powder 10 ~ 15g/L, glucose 3 ~ 8g/L, soybean cake powder 18 ~ 22g/L, fish meal 3 ~ 8g/L, calcium carbonate 5 ~ 9g/L, ammonium sulfate 0.8 ~ 1.2g/L, dipotassium hydrogen phosphate 0.1 ~ 0.5g/L, magnesium sulfate 0.1 ~ 0.3g/L and manganous sulfate 0.1 ~ 0.3g/L, it is 36 ~ 40 DEG C in culture temperature, fermentor tank mixing speed 200 ~ 220rpm cultivates 20 ~ 32h, bacillus subtilis bacterial content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the subtilis then obtained fermenting is prepared into hypopus microbial dry powder.
4. the preparation method of pig as claimed in claim 1, chicken manure just fermenting compound fungus, is characterized in that the preparation of the hypopus microbial dry powder of described Bacillus licheniformis comprises the following steps:
Bacillus licheniformis strain on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 15 ~ 30%, the formula of described activation medium is peptone 8 ~ 12g/L, extractum carnis 1 ~ 5g/L and sodium-chlor 3 ~ 7g/L, be 36 ~ 40 DEG C in culture temperature, shaking flask rotating speed 180 ~ 220rpm cultivates 20 ~ 32h;
The Bacillus licheniformis strain that activation culture is good is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, volume inoculum size is 1 ~ 3%, the formula of described fermention medium is soybean cake powder 13 ~ 17g/L, Semen Maydis powder 13 ~ 17g/L, dipotassium hydrogen phosphate 0.05 ~ 0.15g/L, potassium primary phosphate 0.05 ~ 0.15g/L and calcium chloride 2 ~ 6g/L, it is 36 ~ 40 DEG C in culture temperature, fermentor tank mixing speed 200 ~ 220rpm cultivates 20 ~ 32h, Bacillus licheniformis content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the Bacillus licheniformis then obtained fermenting is prepared into hypopus microbial dry powder.
5. the preparation method of pig as claimed in claim 1, chicken manure just fermenting compound fungus, is characterized in that the preparation of the hypopus microbial dry powder of described Bacillus coagulans comprises the following steps:
Bacillus coagulans strain inoculation on the slant medium cultivate purifying is in the shaking flask of the activation medium of 15 ~ 30% to being equipped with volume ratio, the formula of described activation medium is glucose 10 ~ 12g/L, peptone 8 ~ 12g/L, extractum carnis 1 ~ 5g/L and sodium-chlor 3 ~ 7g/L, be 36 ~ 40 DEG C in culture temperature, shaking flask rotating speed 180 ~ 220rpm cultivates 20 ~ 32h;
By Bacillus coagulans strain inoculation good for activation culture to being equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, volume inoculum size is 1 ~ 3%, the formula of described fermention medium is wheat bran 20 ~ 25g/L, glucose 11 ~ 15g/L, soybean cake powder 12 ~ 18g/L, dipotassium hydrogen phosphate 0.05 ~ 0.15g/L, magnesium sulfate 0.05 ~ 0.15g/L and calcium chloride 2 ~ 6g/L, it is 36 ~ 40 DEG C in culture temperature, fermentor tank mixing speed 200 ~ 220rpm cultivates 30 ~ 40h, Bacillus coagulans content>=10 in fermentor tank
9individual/ml, is then prepared into hypopus microbial dry powder by the Bacillus coagulans list bacterium drying obtained of fermenting.
6. the preparation method of pig as claimed in claim 1, chicken manure just fermenting compound fungus, is characterized in that the preparation of the hypopus microbial dry powder of described bacillus megaterium comprises the following steps:
Bacillus megaterium strain inoculation on the slant medium cultivate purifying is in the shaking flask of the activation medium of 15 ~ 30% to being equipped with volume ratio, the formula of described activation medium is peptone 8 ~ 12g/L, extractum carnis 1 ~ 5g/L and sodium-chlor 3 ~ 7g/L, be 36 ~ 40 DEG C in culture temperature, shaking flask rotating speed 180 ~ 220rpm cultivates 20 ~ 32h;
By bacillus megaterium strain inoculation good for activation culture to being equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, volume inoculum size is 1 ~ 3%, the formula of described fermention medium is soybean cake powder 20 ~ 26g/L, Semen Maydis powder 12 ~ 20g/L, dipotassium hydrogen phosphate 0.05 ~ 0.15g/L, potassium primary phosphate 0.05 ~ 0.15g/L and calcium chloride 2 ~ 6g/L, it is 36 ~ 40 DEG C in culture temperature, fermentor tank mixing speed 200 ~ 220rpm cultivates 20 ~ 32h, bacillus megaterium content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the bacillus megaterium then obtained fermenting is prepared into hypopus microbial dry powder.
7. the preparation method of pig as claimed in claim 1, chicken manure just fermenting compound fungus, is characterized in that the preparation of the hypopus microbial dry powder of described viride comprises the following steps:
Viride bacterial classification on the slant medium cultivate purifying is seeded to and volume ratio is housed is in the shaking flask of the activation medium of 15 ~ 30%, described activation medium is wort potato sucrose substratum, be 28 ~ 30 DEG C, shaking flask rotating speed 140 ~ 180rpm, activation culture 32 ~ 48h in culture temperature;
Viride bacterial classification good for activation culture is inoculated into and is equipped with in fermentor tank that volume ratio is the fermention medium of 50 ~ 70%, inoculum size is volume ratio 1 ~ 3%, the formula of described fermention medium is glucose 8 ~ 12g/L, corn cob 5 ~ 6g/L, calcium chloride 0.2 ~ 0.6g/L, magnesium sulfate 0.1 ~ 0.5g/L, potassium primary phosphate 18 ~ 22g/L, yeast extract paste 8 ~ 12g/L, ferrous sulfate 3 ~ 7mg/L, zinc sulfate 1.0 ~ 1.8mg/L, cobalt chloride 3.5 ~ 4.0mg/L and manganous sulfate 1.2 ~ 1.8mg/L, it is 28 ~ 30 DEG C in culture temperature, fermentor tank mixing speed 140 ~ 180rpm, enlarged culturing 68 ~ 84h, to mould content>=10 of fermentor tank Green wood
8individual/ml, is then prepared into hypopus microbial dry powder by the viride list bacterium obtained of fermenting through lyophilize.
8. use fermenting compound fungus fermented-pig described in claim 1, method that chicken manure just prepares fertilizer, it is characterized in that comprising the following steps:
(1) swine excrement or chicken manure are just mixed with chippy stalk, added to mix by described fermenting compound fungus obtain mixing raw material according to the consumption of 1 ~ 2Kg/t, the moisture of mixing raw material is 50 ~ 65wt%;
(2) the mixing raw material code of step (1) heap is in strip fertile heap, holding temperature is 55 ~ 65 DEG C and starts fermentation, fermentation carries out first time turning two days later, carries out a turning, keep moisture content 50 ~ 65wt% between yeast phase after secondary every 1 ~ 2 day;
(3), after reducing to normal temperature to the fermentation heap temperature of step (2), fermentation ends, obtains the fertilizer that water ratio is 20 ~ 30wt%.
9. fermented-pig as claimed in claim 8, chicken manure just prepare the method for fertilizer, it is characterized in that: between yeast phase, bar shaped fertilizer heap arranges the ventilation hole that multiple diameter is 1.5 ~ 2cm.
10. fermented-pig as claimed in claim 8, chicken manure just prepare the method for fertilizer, it is characterized in that: in described mixing raw material, ight soil is 3 ~ 5:1 ~ 2 with the mixed weight ratio of stalk.
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Application publication date: 20141224 |
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| RJ01 | Rejection of invention patent application after publication |