CN103113167B - Compound microbial organic fertilizer and preparation method thereof - Google Patents

Compound microbial organic fertilizer and preparation method thereof Download PDF

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CN103113167B
CN103113167B CN201310086526.1A CN201310086526A CN103113167B CN 103113167 B CN103113167 B CN 103113167B CN 201310086526 A CN201310086526 A CN 201310086526A CN 103113167 B CN103113167 B CN 103113167B
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fermented liquid
viride
juwei002
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CN103113167A (en
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赵钦刚
孙中涛
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Shandong soil Biotechnology Co., Ltd.
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赵钦刚
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Abstract

The invention relates to a compound microbial organic fertilizer and a preparation method thereof. The compound microbial organic fertilizer is prepared from the following ingredients in parts by weight: 1-4 parts of trichoderma viride JUWEI002 fermentation liquor, 1-4 parts of bacillus subtilis JUWEI001 fermentation liquor, 20-30 parts of turfy soil, 33-38 parts of tobacco residue, 33-38 parts of silkworm faeces, 6-7 parts of calcium powder and 1-2 parts of potassium fulvate. The compound microbial organic fertilizer disclosed by the invention has the advantages that the quality of crops can be remarkably improved, the yield of the crops is increased, and the soil structure is improved.

Description

A kind of compound microbial fertilizer and preparation method thereof
Technical field
The present invention relates to a kind of compound microbial fertilizer and preparation method thereof, particularly a kind ofly add the compound microbial fertilizer of active composite bacteria and potassium fulvate, calcium powder taking tobacco slag and silkworm excrement as main, belong to bio-feritlizer technical field.
Background technology
Soil is the important carrier of plant growth environment, and it provides photosynthetic raw material and attaching substratum to plant, and soil quality and crop yield are closely bound up.The basis of soil quality is soil fertility, and soil fertility is soil for plant-growth provides and coordinate the ability of nutritional condition and envrionment conditions, is the general performance of the various essential propertys of soil.In recent years, along with the rapid growth of population, people, in order to improve crop yield, use chemical fertilizer and agricultural chemicals in a large number, cause organic content reduction, nutrient loss in soil, soil acidification, harden, the plant recovery of nutrient such as nitrogen, phosphorus, potassium are not high, and soil flora is unbalance, biological activity reduces, harmful bacteria and objectionable impurities increase, soil fertility degradation, and agricultural sustainable development is subject to serious challenge.Chemical fertilizer and indiscriminate use of pesticide, both increased peasant's burden, causes again in agricultural-food the objectionable impuritiess such as chemical residue to roll up, and had a strong impact on the mankind's healthy and existence.So Organic farming, Agricultural product without pollution are more and more subject to people's attention, and biological organic fertilizer is the hope place of Organic Farming due to its unique biological activity with to the improved characteristics of soil.
Turfy soil is the product in formation process of bog, contains large quantity of moisture and the plant residue thoroughly not decomposed, soil ulmin and a part of mineral substance.Turfy soil organic content is more than 30%, quality is soft to be easy to fragmentaryly, has light weight, water holding, ventilative feature, has the irreplaceable effect of other organic materials, be the excellent carrier of adsorption function microbial inoculum, produce widespread use in green compoiste fertilizer in China in recent years.
China is tobacco big producing country, and annual yield of tobacco 450~5,000,000 ton, wherein can produce nearly 25% the discarded tankage such as tobacco leaf, offal.In tobacco slag, total nitrogen content reaches 10%, the content of potassium, phosphorus, calcium, organic matter, all in 1% left and right, also contains the antimicrobial substance such as nicotine, chlorogenic acid, not only can supply nutrients for farm crop, also having the effect that prevents disease and pest and suppress weed growth, is the high quality raw material of making fertilizer.But the useful bacterial strain in soil is murdered in the too high meeting of nicotine content, can cause the underproduction of farm crop.
Microbial organic fertilizer is on the basis of traditional organic fertilizer, to inoculate beneficial microorganism and a kind of fertilizer of producing, and compared with traditional fertilizer, that microbial organic fertilizer has is quick-acting, long-acting, disease-resistant, improvement soil and anti-hardening function.Microbial organic fertilizer can be at plant growth Quick in early stage for the required available nutrient of crop, again can be in process of crop growth, decompose organic and mineral substance release nutrient by the vital movement of microorganism, constantly supply crop growth needs, bring into play the quick-acting and long-acting effect having concurrently.Biological organic fertilizer is except containing the necessary macroelement of crop growthing development and trace element, also contain a large amount of organic matters, microbial metabolites and a large amount of active beneficial microorganism, can promote plant growth, can strengthen again crop resistance, ensure the raising of crop yield.A large amount of organic matters and beneficial microorganism that microbial organic fertilizer is contained, can improve Soil structure, and rehabilitating soil micro-flora suppresses harmful microbe and spreads, and safeguards soil ecology balance.
The biological organic fertilizer of China has broad variety at present, but that be really widely used and few, main problem is the processes such as microbial organic fertilizer needs by fermentation, decomposition, also need to add or retain appropriate beneficial microorganism, also there is the defect that cost is higher, quality is stable not in biological organic fertilizer in addition simultaneously.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of pollution-free, efficient, practical compound microbial fertilizer is provided, the present invention also provides the application method of this microbial organic fertilizer.
Technical solution of the present invention is as follows:
A kind of compound microbial fertilizer, component is as follows, is weight part:
1~4 part of viride (Trichoderma viride) JUWEI002 fermented liquid, 1~4 part of subtilis (Bacillus subtilis) JUWEI001 fermented liquid, 20~30 parts of turfy soils, 33~38 parts of tobacco slags, 33~38 parts of silkworm excrements, 6~7 parts of calcium powders, 1~2 part of potassium fulvate;
Viable count>=9 × 10 in described viride (Trichoderma viride) JUWEI002 fermented liquid 8individual/ml, described viride (Trichoderma viride) JUWEI002 is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number is CGMCC NO.7035, the preservation time is on December 24th, 2012, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica;
Viable count>=9 × 10 in described subtilis (Bacillus subtilis) JUWEI001 fermented liquid 8individual/ml; Made by subtilis (Bacillus subtilis) JUWEI001 fermentation, subtilis (Bacillus subtilis) JUWEI001 is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number is CGMCC NO.7034, the preservation time is on December 24th, 2012, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
Preferred according to the present invention, described potassium fulvate specification is: fineness < 120 orders, water-soluble substance >=99.7%, water-soluble xanthohumic acid >=50%, potassium oxide 9.5~10%.
Preferred according to the present invention, described turfy soil fineness is 120 orders.
Preferred according to the present invention, that described tobacco slag is selected is fresh, without the tobacco slag going mouldy, make after adopting commercially available high-quality decomposing agent fermentation maturity.Tobacco slag is the tankage of cigarette industry, mainly contains Mierocrystalline cellulose, starch, protein, sugar, alkaloid, pectin, carbohydrate, phenol, lipid acid, terpenes, sterol and inorganic substance.
Preferred according to the present invention, that described silkworm excrement is selected is fresh, without the silkworm excrement going mouldy, make after adopting commercially available high-quality decomposing agent fermentation maturity.Silkworm excrement is the ight soil of nibbling after mulberry leaf, particulate state, air-dry after, surperficial grey black or taupe brown, matter is hard, non-friable (easily fragmentary after wet); Micro-have green grass gas (mulberry leaf delicate fragrance), and its main component is the organic substance such as Mierocrystalline cellulose, protein.
Preferred according to the present invention, described calcium powder is oyster shell whiting.
The preparation method of above-mentioned compound microbial fertilizer, step is as follows:
(1) viride JUWEI002 fermented liquid and subtilis JUWEI001 fermented liquid are mixed, make mixed solution;
(2) by 80~100 DEG C of hot-air seasoning 120min for turfy soil, make degerming turfy soil;
(3) the degerming turfy soil mixing and absorption that mixed solution step (1) being made and step (2) make, through pulverizing, cross 100 mesh sieves, then mix with tobacco slag and silkworm excrement, and add calcium powder and potassium fulvate, mix, with the hot blast cryodrying lower than 40 DEG C to water content lower than 15%(w/w), make compound microbial fertilizer.
Preferred according to the present invention, described viride JUWEI002 fermented liquid is prepared as follows:
1. be inoculated in dull and stereotyped PDA substratum with viride JUWEI002;
2. picking colony edge mycelia is transferred in wheat bran juice substratum, 28 DEG C~30 DEG C of culture temperature, and 200rpm, incubation time 30~40h, makes viride JUWEI002 nutrient solution;
3. with 5%(w/w) inoculum size viride JUWEI002 nutrient solution that 2. step is made be inoculated in wheat bran juice fermention medium, fermentation condition is: 28~30 DEG C of temperature, tank pressure: 0.1Mpa, 0.8~1vvm ventilates, stirring velocity 180~200rpm, incubation time is 6~8 days, makes viride JUWEI002 fermented liquid.In viride JUWEI002 fermented liquid, viable count should be not less than 9 × 10 8individual/ml.
The dull and stereotyped PDA substratum of above-mentioned steps in 1., every liter of component is as follows: potato 200g, sucrose 20g, agar 20g, distilled water 1000mL;
The wheat bran juice nutrient media components of above-mentioned steps in is 2. as follows, is all weight percentage: wheat bran 10%, sucrose 5%, excess water, natural pH value;
The wheat bran juice fermention medium component of above-mentioned steps in is 3. as follows, is all weight percentage: wheat bran 6%, sucrose 3%, excess water, natural pH value.
Preferred according to the present invention, described subtilis JUWEI001 fermented liquid is prepared as follows:
A, by subtilis JUWEI001, streak inoculation is cultivated 45~50h in 37 DEG C of beef-protein mediums;
B, transfering loop picking thalline are inoculated in Semen Maydis powder liquid nutrient medium, and 180~200rpm cultivates 22~26h for 37 DEG C;
C, with 1%(w/w) inoculum size is inoculated in fermentation of corn starch substratum, carries out fermentation culture, fermentation condition is: 38 DEG C of temperature, tank pressure: 0.1Mpa, the 0.8~1vvm that ventilates, stirring velocity 180~200rpm, incubation time is 3~4 days, makes subtilis JUWEI001 fermented liquid.In subtilis JUWEI001 fermented liquid, effectively cell concentration should be not less than 3 × 10 9individual/ml.
Every hundred milliliters of components of beef-protein medium in above-mentioned steps a are as follows: extractum carnis 0.3g, peptone 1g, sodium-chlor 0.5g, agar 2g, distilled water are settled to 100ml, pH7.0~7.2;
Every hundred milliliters of components of Semen Maydis powder liquid nutrient medium in above-mentioned steps b are as follows: Semen Maydis powder 1.5g, bean cake powder 0.9g, Sodium phosphate dibasic 0.4g, potassium primary phosphate 0.03g, MgSO 40.1%, distilled water is settled to 100ml, pH6.8~7.0;
Fermentation of corn starch substratum in above-mentioned steps c, component is as follows, is all weight percentage: Semen Maydis powder 2.00%, bean cake powder 7.00%, Sodium phosphate dibasic 0.40%, potassium primary phosphate 0.03%, MgSO 40.1%, defoamer 0.02~0.005%, pH6.8~7.0.
Mentioned reagent if no special instructions, is conventional commercially available prod, this area.
Beneficial effect
1, the present invention selects the function yeast of subtilis (Bacillus subtilis) JUWEI001 as microbial organic fertilizer, this bacterial strain strong stress resistance, in process of growth, can produce the abundant physiologically active substance with high vigor and control of plant disease function, there is promotion decomposition of soil organic matter, improve the fertile ability of soil control and soil enzyme activities, improve Soil Microorganism flora structure, promote plant root growth, suppress the effects such as harmful fungoid, bacterium, virus and bacterium substance.
2, viride (Trichoderma viride) JUWEI002 that the present invention selects is as the function yeast of microbial organic fertilizer, and growth potential is strong, and adaptability is extremely strong, all can Fast Growth under multiple edatope, can capture fast site, space and nutritional resource.This bacterium can produce the multiple bioactive enzyme system that has, and as cellulase, chitinase, zytase etc., can decompose the soil organism, dissolves soluble substance, can promote the absorption of plant to mineral substance, Promoting plant growth; Viride (Trichoderma viride) JUWEI002 can effectively prevent and treat soil-borne disease; The mycelia of this bacterial strain can adhere to and hand over around soil particle, improves soil stability and permeability, breaks soil compaction.
3, subtilis JUWEI001 and viride JUWEI002 use jointly as the function yeast of microbial organic fertilizer, it is fast that the two all has bacterial strain reproduction speed, exocytosis activity is high, can utilize the scale operation of liquid submerged fermentation technology, thereby obtain fast a large amount of thalline and spore, reduce production costs; In addition, the morphological properties of two kinds of bacterial classifications is different, is suppressing Plant diseases, improve soil physico-chemical property, promote the growth of root system of plant, strengthen the receptivity of root system to nutritive ingredient, regulate rhizosphere soil microorganism flora structure many-side, can play complementation and enhancement.
4, the present invention selects turfy soil as function yeast adsorbing base, can improve the physical behavior of soil, improve soil organic matter content, promote the growth of plant root, promote farm crop absorbing nutritive elements such as nitrogen, phosphorus, potassium, thereby promote growing of farm crop, improve the yield and quality of farm crop.
5, the present invention selects tobacco slag and the silkworm excrement materials as decomposed organic matter.First use the scarp after extraction nicotine, then decomposing excessive nicotine by fermentative processing then mixes with the silkworm excrement that is rich in sugar, protein and mineral substance after the fermentative action of becoming thoroughly decomposed, nutraceutical matrix is complete, can fully be absorbed by farm crop, has obvious effect on increasing crop yield.In addition can also effectively improve chlorophyll, vitamin contents, reduce the content of Nitrate in Vegetable, thereby improve the quality of products safety; And tobacco slag and silkworm excrement abundant raw material, animal-origin organic matter and plant origin organic matter are used in conjunction with, and nutrient and beneficiating ingredient complementation are more favourable to plant growth.
6, in the present invention, add calcium powder as auxiliary materials, can supplement plant on the one hand calcareous, there is on the other hand the effect of improvement soil and extra-nutrition, increase soil aggregate, reduce soil compaction.Calcium powder is stability weakly alkaline, and all right sweetening of the soil acidic substance reduce heavy metal and cause harm
7, the potassium fulvate that the present invention adds includes the multiple nutritional components such as trace element, rare earth element, plant-growth regulator, viral inhibitors, makes that fertilizer nutrient is more sufficient, supply is more reasonable; In addition, potassium fulvate can slow release fertilizer, improves chemical fertilizer and agricultural chemicals utilization; Can also accelerate precipitation and decompose, improve Soil structure; Potassium fulvate can also excite plant biological activity, improves the absorption of Plant To Nutrient, promotes plant germination and growth.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is described further, but institute of the present invention protection domain is not limited to this.
Raw material explanation
Described in embodiment 1, viride JUWEI002 fermented liquid is to make by prior art fermentation with viride (Trichoderma viride) JUWEI002 that culture presevation number is CGMCC NO.7035, subtilis JUWEI001 fermented liquid viable count>=1 × 10 9individual/ml; Described in embodiment 2~3, viride JUWEI002 fermented liquid is prepared as follows:
1. be inoculated in dull and stereotyped PDA substratum with viride JUWEI002,2 generations of switching;
2. picking colony edge mycelia is transferred in wheat bran juice substratum, 28 DEG C~30 DEG C of culture temperature, and 200rpm, incubation time 36h, makes viride JUWEI002 nutrient solution;
3. with 5%(w/w) inoculum size viride JUWEI002 nutrient solution that 2. step is made be inoculated in wheat bran juice fermention medium, fermentation condition is: 28~30 DEG C of temperature, tank pressure: 0.1Mpa, 0.8~1vvm ventilates, stirring velocity 180~200rpm, incubation time is 7 days, and in fermented liquid, viable count is 9 × 10 8individual/ml.
The dull and stereotyped PDA substratum of above-mentioned steps in 1., every liter of component is as follows: potato 200g, sucrose 20g, agar 20g, distilled water 1000mL;
The wheat bran juice nutrient media components of above-mentioned steps in is 2. as follows, is all weight percentage: wheat bran 10%, sucrose 5%, excess water, natural pH value;
The wheat bran juice fermention medium component of above-mentioned steps in is 3. as follows, is all weight percentage: wheat bran 6%, sucrose 3%, excess water, natural pH value.
Subtilis JUWEI001 fermented liquid is to make by prior art fermentation with subtilis (Bacillus subtilis) JUWEI001 that culture presevation number is CGMCC NO.7034, and in subtilis JUWEI001 fermented liquid, viable count is 1 × 10 9individual/ml; Described in embodiment 2~3, subtilis JUWEI001 fermented liquid is prepared as follows:
A, by subtilis JUWEI001, streak inoculation is cultivated 48h in 37 DEG C of beef-protein mediums;
B, transfering loop picking thalline are inoculated in Semen Maydis powder liquid nutrient medium, and 180rpm cultivates 24h for 37 DEG C;
C, with 1%(w/w) inoculum size is inoculated in fermentation of corn starch substratum, carries out fermentation culture, fermentation condition is: 38 DEG C of temperature, tank pressure: 0.1Mpa, ventilation 0.8-1vvm, stirring velocity 180~200rpm, incubation time is 3 days, effectively cell concentration is 3 × 10 9individual/ml.
Every hundred milliliters of components of beef-protein medium in above-mentioned steps a are as follows: extractum carnis 0.3g, peptone 1g, sodium-chlor 0.5g, agar 2g, distilled water are settled to 100ml, pH7.0~7.2;
Every hundred milliliters of components of Semen Maydis powder liquid nutrient medium in above-mentioned steps b are as follows: Semen Maydis powder 1.5g, bean cake powder 0.9g, Sodium phosphate dibasic 0.4g, potassium primary phosphate 0.03g, MgSO 40.1%, distilled water is settled to 100ml, pH6.8~7.0;
Fermentation of corn starch substratum in above-mentioned steps c, component is as follows, is all weight percentage: Semen Maydis powder 2.00%, bean cake powder 7.00%, Sodium phosphate dibasic 0.40%, potassium primary phosphate 0.03%, MgSO 40.1%, defoamer (trade(brand)name: th085 steeps enemy, purchased from Tianjin Guan Qian chemical industry sales department) 0.02~0.005%, pH6.8~7.0.
Turfy soil is liked peat development company limited purchased from Manchu Autonomous County of Qingyuan ten thousand, and this peat composed of rotten mosses ash fineness is 120 orders;
Calcium powder is the oyster shell whiting purchased from Shandong starfish feed factory;
Potassium fulvate is purchased from Dongyang, Qingzhou in Shandong province Chemical Co., Ltd., this potassium fulvate fineness < 120 orders, water-soluble substance > 99.7%, water-soluble xanthohumic acid > 50%, potassium oxide 9.5~10%.
Described tobacco slag is that scarp purchased from Yishui County Bo Ruida biochemical industry limited liability company is made through the decomposing agent of the biotechnology company limited that gets profit purchased from Tai'an letter 10d that becomes thoroughly decomposed at 37~45 DEG C.
Described silkworm excrement be by purchased from Shandong soil energetically the silkworm excrement at fertilizer raw material supply center make through the decomposing agent of biotechnology company limited of getting profit purchased from Tai'an letter 10d that becomes thoroughly decomposed at 37~45 DEG C.
Embodiment 1
A kind of compound microbial fertilizer, component is as follows, is weight part:
1 part of viride JUWEI002 fermented liquid, 1 part of subtilis JUWEI001 fermented liquid, 20 parts of turfy soils, 35 parts of tobacco slags, 35 parts of silkworm excrements, 7 parts of calcium powders, 1 part of potassium fulvate.
The preparation method of above-mentioned compound microbial fertilizer, step is as follows:
(1) viride JUWEI fermented liquid and subtilis JUWEI fermented liquid are mixed, make mixed solution;
(2) by 80~100 DEG C of hot-air seasoning 120min for turfy soil, make degerming turfy soil;
(3) the degerming turfy soil mixing and absorption that mixed solution step (1) being made and step (2) make, through pulverizing, cross 100 mesh sieves, then mix with scarp and silkworm excrement, and add calcium powder and potassium fulvate, mix, with be less than 40 DEG C of hot blast cryodryings to water content lower than 15%(w/w), make compound microbial fertilizer.
Apply above-mentioned compound microbial fertilizer, compare as fertilizer with common Chicken dung, the impact (Jinan, Shandong Province) on tomato yield and quality.Test group: use the compound microbial fertilizer of 200kg/ mu, control group: routine is used Chicken dung 400kg/ mu, tomato variety is that gingko is rich by force, by line-spacing 35cm, spacing in the rows 30cm, the high 15cm in ridge, Routine Management.
Result, as shown in table 1,2, can increase tomato yield after using this product, shortens Time To Market, can also improve fruit quality, reduces the sickness rate of bacterial wilt of tomato.
The impact of this product of table 1 on tomato yield and bacterial wilt natural occurrence rate
The impact of this product of table 2 on Quality of Tomato Fruit
Embodiment 2
A kind of compound microbial fertilizer, component is as follows, is weight part:
1 part of viride JUWEI002 fermented liquid, 1 part of subtilis JUWEI001 fermented liquid, 22 parts of turfy soils, 38 parts of tobacco slags, 38 parts of silkworm excrements, 6 parts of calcium powders, 1 part of potassium fulvate.
Preparation method is with embodiment 1.
The compound microbial Organic Fertilizers In Continuous Cropping greenhouse watermelon growth that the present embodiment makes and the impact (Heze City, Shandong Province) of rhizospheric microorganism.Be glad No. 1 of capital for examination variety of watermelon, using the compound microbial fertilizer of using 200kg/ mu as test group; With commercially available biological organic fertilizer as a control group, amount of application is 200kg/ mu.Duration of test, all processing are not all used any sterilant and other base manure.
As shown in Table 3, 4, this product can improve nutrient content in soil to result, promotes the release of soil nutrient to improve Soil Microorganism quantity, increases microbial diversity, and this product effect is better than contrasting biological organic fertilizer.This product has obviously increased the yield of watermelon as shown in Table 5,6, improves quality of watermelon, reduces watermelon blight occurring degree.
The impact of this product of table 3 on continuous cropping watermelon rhizosphere soil microorganism
The impact of this product of table 4 on continuous cropping watermelon shed soil nutrient content
The impact of this product of table 5 on continuous cropping the yield of watermelon and quality
The impact of this product of table 6 on continuous cropping watermelon blight sickness rate
Embodiment 3
A kind of compound microbial fertilizer, component is as follows, is weight part:
4 parts of viride JUWEI002 fermented liquids, 4 parts of subtilis JUWEI001 fermented liquids, 25 parts of turfy soils, 33 parts of tobacco slags, 33 parts of silkworm excrements, 7 parts of calcium powders, 1 part of potassium fulvate.
Preparation method is with embodiment 1.
The impact (Shandong Weifang) of compound microbial fertilizer on Yield of Strawberry and quality.Test kind is the precious early life of handing over.Test group 1 is used this product 200kg/ mu, and test group 2 is used commercially available microbial organic fertilizer 200kg/ mu, control group applications of farmyard manure (hog manure) 400kg/ mu.Three kinds of fertilizer apply as base manure is disposable, protection ground conventional cultivation.
Result is as shown in table 7, and compared with applications of farmyard manure, this product and commercially available microbial organic fertilizer can improve strawberry sugar degree, improve color and luster, and effect quite; This product is better than commercially available prod to the effect of strawberry volume increase.
The impact of this product of table 7 on Yield of Strawberry and quality

Claims (10)

1. a compound microbial fertilizer, is characterized in that, component is as follows, is weight part:
Viride ( trichoderma viride) 1~4 part of JUWEI002 fermented liquid, 1~4 part of subtilis JUWEI001 fermented liquid, 20~30 parts of turfy soils, 33~38 parts of tobacco slags, 33~38 parts of silkworm excrements, 6~7 parts of calcium powders, 1~2 part of potassium fulvate;
Viable count>=9 × 10 in described viride JUWEI002 fermented liquid 8individual/ml, described viride JUWEI002 is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number is CGMCC NO.7035, the preservation time is on December 24th, 2012, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica;
Viable count>=9 × 10 in described subtilis JUWEI001 fermented liquid 8individual/ml; Made by subtilis JUWEI001 fermentation, subtilis JUWEI is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number is CGMCC NO.7034, the preservation time is on December 24th, 2012, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
2. compound microbial fertilizer as claimed in claim 1, is characterized in that, described potassium fulvate specification is: fineness < 120 orders, water-soluble substance >=99.7%, water-soluble xanthohumic acid >=50%, potassium oxide 9.5~10%.
3 .compound microbial fertilizer as claimed in claim 1, is characterized in that, described turfy soil fineness is 120 orders.
4 .compound microbial fertilizer as claimed in claim 1, is characterized in that, that described tobacco slag is selected is fresh, without the tobacco slag going mouldy, and makes after adopting commercially available high-quality decomposing agent fermentation maturity;
That described silkworm excrement is selected is fresh, without the silkworm excrement going mouldy, make after adopting commercially available high-quality decomposing agent fermentation maturity.
5 .compound microbial fertilizer as claimed in claim 1, is characterized in that, described calcium powder is oyster shell whiting.
6 .described in claim 1, the preparation method of compound microbial fertilizer, is characterized in that, step is as follows:
(1) viride JUWEI002 fermented liquid and subtilis JUWEI001 fermented liquid are mixed, make mixed solution;
(2) by 80~100 DEG C of hot-air seasoning 120min for turfy soil, make degerming turfy soil;
(3) the degerming turfy soil mixing and absorption that mixed solution step (1) being made and step (2) make, through pulverizing, cross 100 mesh sieves, then mix with tobacco slag and silkworm excrement, and add calcium powder and potassium fulvate, mix, with lower than 40 DEG C of hot blast cryodryings to water content lower than 15%(w/w), make compound microbial fertilizer.
7 .preparation method as claimed in claim 6, is characterized in that, described viride JUWEI002 fermented liquid is prepared as follows:
1. be inoculated in dull and stereotyped PDA substratum with viride JUWEI002;
2. picking colony edge mycelia is transferred in wheat bran juice substratum, 28 DEG C~30 DEG C of culture temperature, and 200rpm, incubation time 30~40h, makes viride JUWEI002 nutrient solution;
3. with 5%(w/w) inoculum size viride JUWEI002 nutrient solution that 2. step is made be inoculated in wheat bran juice fermention medium, fermentation condition is: 28~30 DEG C of temperature, tank pressure: 0.1Mpa, 0.8~1vvm ventilates, stirring velocity 180~200rpm, incubation time is 6~8 days, makes viride JUWEI002 fermented liquid.
8 .preparation method as claimed in claim 7, is characterized in that, the dull and stereotyped PDA substratum of described step in 1., and every liter of component is as follows: potato 200g, sucrose 20g, agar 20g, distilled water 1000mL;
The wheat bran juice nutrient media components of described step in is 2. as follows, is all weight percentage: wheat bran 10%, sucrose 5%, excess water, natural pH value;
The wheat bran juice fermention medium component of described step in is 3. as follows, is all weight percentage: wheat bran 6%, sucrose 3%, excess water, natural pH value.
9 .preparation method as claimed in claim 6, is characterized in that, described subtilis JUWEI001 fermented liquid is prepared as follows:
A, by subtilis JUWEI001, streak inoculation is cultivated 45~50h in 37 DEG C of beef-protein mediums;
B, transfering loop picking thalline are inoculated in Semen Maydis powder liquid nutrient medium, and 180~200rpm cultivates 22~26h for 37 DEG C;
C, with 1%(w/w) inoculum size is inoculated in fermentation of corn starch substratum, carries out fermentation culture, fermentation condition is: 38 DEG C of temperature, tank pressure: 0.1Mpa, the 0.8~1vvm that ventilates, stirring velocity 180~200rpm, incubation time is 3~4 days, makes subtilis JUWEI001 fermented liquid.
10 .preparation method as claimed in claim 9, is characterized in that, the every hundred milliliters of components of beef-protein medium in described step a are as follows: extractum carnis 0.3g, peptone 1g, sodium-chlor 0.5g, agar 2g, distilled water are settled to 100ml, pH7.0~7.2;
Every hundred milliliters of components of Semen Maydis powder liquid nutrient medium in described step b are as follows: Semen Maydis powder 1.5g, bean cake powder 0.9g, Sodium phosphate dibasic 0.4g, potassium primary phosphate 0.03g, MgSO 40.1%, distilled water is settled to 100ml, pH6.8~7.0;
Fermentation of corn starch substratum in described step c, component is as follows, is all weight percentage: Semen Maydis powder 2.00%, bean cake powder 7.00%, Sodium phosphate dibasic 0.40%, potassium primary phosphate 0.03%, MgSO 40.1%, defoamer 0.02~0.005%, pH6.8~7.0.
CN201310086526.1A 2013-03-18 2013-03-18 Compound microbial organic fertilizer and preparation method thereof Active CN103113167B (en)

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