CN107586200A - The preparation method of one plant nutrient liquor - Google Patents
The preparation method of one plant nutrient liquor Download PDFInfo
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- CN107586200A CN107586200A CN201710938492.2A CN201710938492A CN107586200A CN 107586200 A CN107586200 A CN 107586200A CN 201710938492 A CN201710938492 A CN 201710938492A CN 107586200 A CN107586200 A CN 107586200A
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Abstract
The invention discloses the preparation method of a plant nutrient liquor; the nutrient solution is utilized in shell containing abundant marine bioactivity calcium, keratin and its hydrolysing activity peptide, taurine; addition modification of chitosan, humic acid, polyacrylamide, pitch breast, peanut shell powder calcium superphosphate, chicken manure, calcium superphosphate, borax, zeolite powder, activated carbon, the dregs of a decoction, powder of straw, wood chip, paper mill sludge are prepared; the release of active material can not only be controlled; meet the nutritional need of plant; improve the disease-resistant performance of plant, moreover it is possible to increase the water retention property of soil.The plant nutrition liquid has the characteristics of resource reutilization, has good development prospect by the use of discarded shell as main matter.
Description
Technical field
The invention belongs to plant nutrient development technique field, more particularly to a kind of system of shell active material plant nutrition liquid
Preparation Method.
Background technology
The safety of soil environment problem that China faces at present becomes increasingly conspicuous.Nothing is destroyed or be contaminated to soil environment
Influence can be brought to the ecological functions of soil by doubting, in some instances it may even be possible to lose soil system collapse, function.Soil pollution does not only result in
The reduction of soil quality and productivity, and cause the decline of water, gas environmental quality, serious soil pollution will be injured directly
Ecological safety, food security and human-body safety；Although at present in peripheral doses, chemical remediation and the engineering technology of pollution environment
Aspect has certain progress, and forms some practical techniques.But these restorative procedures often break the thing of meeting contaminated site
Rationality matter, or even the secondary pollution of environment can be caused, the reparation for soil pollution is not inherently improved and solved.
Conceding the land to forestry has become a kind of national conditions, however, on the soil that soil has deteriorated, many plants are difficult to grow.Plant is sought
Nutrient solution can effectively improve the fertility for deteriorating soil, improve the resistance against diseases of plant, can make soil of the plant faster in deterioration
Upper quick healthy growth.
A kind of multi-functional plant nutrient solutions of patent of invention CN201610868455.4, by various trace elements, amino acid, boron
Sand, tankage, peanut press pulp, soya bean, deep sea fish oil, camphor, zinc phosphide, manganese oxide, Fructus meliae toosendan, peppery part, rare earth, sulfuric acid are sub-
The battalion for the microemulsion dosage that iron, potassium dihydrogen phosphate, acetic acid sodium dihydrogen, urea, potash fertilizer, phosphoric acid, glucose, water, emulsifying agent are prepared into
Nutrient solution.The multi-functional plant nutrient solution of the present invention can have simultaneously to be provided nutrient, synergy, the loss for controlling fertilizer nutrient and waves
Hair, promote the effect of plant is to the sorption enhanced of nutrient and utilization, Resistant, antibacterial, suitable on the basis of having liquid fertilizer,
Inorganic fertilizer auxiliary agent is added, mixed organic fertilizer is made, it is also possible to manufacture the addition auxiliary agent of biological pesticide.Patent of invention
A kind of ornamental plant nutrient solutions of CN201710468313.3, by urea, MAP, potassium nitrate, calcium nitrate, machine discarded object, bud
Spore bacillus fermentation thing, agricultural crop straw and water composition.Compared with prior art, the present invention by fermenting crops straws make and
Into, environmental protection, nutrient is added in addition, not only made the nutrient solution for adapting to ornamental plant, while organic fertilizer has been made, especially
It is applied to ornamental plant, has the value of popularization and application.The plant nutrient liquors of patent of invention CN201610313949.6 mono-, by
Potassium nitrate, urea, ammonium nitrate, magnesium sulfate, ammonium dihydrogen phosphate, calcium nitrate, manganese sulfate, zinc sulfate, ironic citrate, boric acid, molybdic acid
Sodium, sodium iodide, folic acid, asparagine, glucose, amino acid, multidimensional, collagen, capsicum water, deionized water composition；The battalion
Nutrient solution raw material is easy to get, and makes simple, and cost is low, green safe, nutritious, meet nutrition needed for plant growth each stage, promotees
Enter growth, effective pre- insect-pest is long-term use of, is advantageous to the growth of plant.In the prior art, the utilization to shell focus mostly in
Calcium activated is prepared after calcining, and other organic substances in shell such as keratin, taurine etc. can not be utilized.
China's shellfish culture starts from the early 1970s, according to statistics, at the beginning of the eighties, the annual production of shellfish culture is 300,000
Ton or so, increases to 2,000,000 tons or so the beginning of the nineties, then fast development.With the increase of shellfish culture yield, freeze shellfish post and do
Bei Zhu processing capacity increase, resulting scallop processing waste increase, and shellfish processing waste (including internal organ) accounts for whole
The 30% of individual shellfish, at present fraction shellfish processing waste undersold as shrimp feed, largely abandon and be allowed to corruption in seashore
It is rotten, cause the wasting of resources and environmental pollution.Not only contain abundant protein, fat, vitamin, micro- in shellfish processing waste
The nutritional ingredients such as secondary element, also containing the various bioactivators such as active polysaccharide, taurine, EPA, DHA, nutritive value is high,
And antitumor, antiviral, the anti-ageing different physiological roles of waiting for a long time of tool.Therefore, to its processing and utilization, its added value is improved, it has also become
Urgent problem to be solved.
In the prior art, the report that plant nutrient is prepared using shell active material is fewer.
The content of the invention
The defects of to make up in the prior art, the present invention utilize active material in shell to prepare plant nutrition liquid, the present invention
A kind of method for additionally providing high efficiency extraction shell calcium activated, keratin, active peptide and amino acid.
The preparation method of one plant nutrient liquor, is mainly prepared by following steps：
A. pretreatment of raw material
（1）Choose high-quality without the great shell to go mouldy, clean up, dry, be crushed to 200 ~ 250 mesh and obtain oyster shell whiting；
（2）Take 100 parts of oyster shell whitings with water according to solid-liquid ratio 1:(10 ~ 20) it is well mixed, then adds neutral proteinase, leads to simultaneously
Entering high-pressure pulse electric, setting electric-field intensity is 30 ~ 35Kv/cm, umber of pulse 15 ~ 20, and processing time is 5 ~ 10min/h, 50 ~ 60
DEG C heating water bath carries out 12 ~ 24h of enzymolysis, obtains enzymolysis liquid 1；
B. the preparation of active component
（1）Enzymolysis liquid 1 is centrifuged into 10 ~ 15min under 10000rpm, takes supernatant 1 standby, takes precipitation, is added in mass ratio
Enter glucose 1.4 ~ 2.0%, corn extract 1.6 ~ 2.0%, potassium dihydrogen phosphate 0.2 ~ 0.5%, sodium chloride 0.5 ~ 1%, regulation pH is 7
~ 8, it is put into fermentation tank, adds water to the 60 ~ 70% of fermenter volume, after high pressure steam sterilization, be inoculated with compound bacteria, fermentation ginseng is set
Number is 180 ~ 200r/min of agitation revolution, filtrated air 1 ~ 1.5L/min of throughput, 37 ~ 40 DEG C of fermentation temperature, is fermented 7 ~ 10 days,
Obtain zymotic fluid；
The compound bacteria is bacillus licheniformis and lactic acid bacteria；
（2）Zymotic fluid is taken, with lemon acid for adjusting pH to 6.5 ~ 7.5,10 ~ 15min is centrifuged under 5000rpm, takes supernatant 2
It is standby；Take precipitation, by its quality 8 ~ 10% add it is a kind of in SM98011 protease, alkali protease, SM97010 protease or
More than one combinations, 5 ~ 6h is digested in 50 ~ 60 DEG C, 130rpm shaking baths, obtains enzymolysis liquid 2；
SM98011 protease, alkali protease, SM97010 protease can effectively decompose keratin generation active peptide, protease
Also the active component in soil conditioner, energy are further improved in SM98011 enzymolysis product containing abundant flavor amino acid
More nutriments easily absorbed are provided for soil beneficial bacterium.
C. the preparation of plant nutrition liquid
（1）Supernatant 1, supernatant 2, enzymolysis liquid 1 and enzymolysis liquid 2 are mixed, by its weight fraction than add chitosan solution 40 ~
50 parts, 10 ~ 15 parts of humic acid, 10 ~ 15 parts of polyacrylamide, 10 ~ 15 parts of pitch breast, 10 ~ 15 parts of peanut shell powder is well mixed, so
After drying 4 ~ 6h in 50 ~ 60 DEG C of blast driers, cross 100 mesh sieves and powder is made；
With soil polymerisation can occur for polyacrylamide rapidly in the presence of water, make soil loose quickly, not only be beneficial to battalion
The diffusion of element and organic substance is supported, also solves microbial bacteria in hardened soil because of the irreproducible problem of anoxic；
On the other hand, polyacrylamide can improve the water holding capacity of soil；In the present invention, polyacrylamide is also used as emulsifying agent, drop
Frictional force in low mixed liquor between each material, improve the stability of granulation.
Pitch breast can suppress soil water evaporation, and the bigger inhibition of dosage is better, but the infiltration covering speed of pitch breast
Rate is low, it is impossible to soon penetrates into deep soil and plays a role；And polyacrylamide can effectively facilitate the diffusion of pitch breast,
Improve the efficiency of pitch breast.
（2）Polyethylene glycol is dissolved at 70 ~ 75 DEG C, by polyethylene glycol, anhydrous citric acid powder and mercaptoethanol solution
Mass ratio is 2:1:1~4:3:2 are mixed, and crush mixture after then adding cooling, are crossed 80 mesh sieves and are obtained acid source；It is described
Mercaptoethanol solution concentration is 10 ~ 15mmol/L；
Mercaptoethanol can open the disulfide bond in remaining keratin macromolecular in calcium activated, promote it to be completely dissolved, so as to improve
Calcium activated biomass content, improve content of peptides；The mercaptoethanol of low concentration can promote keratolysis, the sulfydryl second of high concentration
Alcohol can suppress the dissolving of keratin.
（3）Chicken manure, calcium superphosphate and borax are mixed, pH to 5 ~ 7 is adjusted after being down to room temperature, is subsequently added into complex enzyme formulation
Stir, be sprinkled into zeolite powder, activated carbon, heating is digested, then is warming up to 35 ~ 38 DEG C and is digested, enzymolysis time 3 ~ 6
My god, then be warming up to 130 ~ 140 DEG C of inactivations and obtain material 1；
The complex enzyme formulation is by cellulase and amylase according to 2:1~2:3 combine；
（4）After the dregs of a decoction, powder of straw, wood chip, paper mill sludge are mixed, crush, add polyvinyl alcohol and be uniformly mixing to obtain material
2；
（5）Powder, acid source, material 1, material 2 are well mixed, 5 ~ 6% Macrogol 6000 solution is added in mass ratio, mixes
Close uniform, you can obtain plant nutrition liquid.
As a further improvement, the neutral protease vigor is 20 ~ 300,000 u/g, enzyme dosage is 7 ~ 10%.
As a further improvement, the compound bacteria inoculation method is：Bacillus licheniformis is first inoculated with, after fermenting 3 ~ 4 days, is connect
Kind lactic acid bacteria, adds sucrose, continues fermentation to 7 ~ 10 days, obtains zymotic fluid；The bacillus licheniformis inoculum concentration is 7 ~ 10%,
Lactobacillus inoculum amount is 5 ~ 8%, and sucrose addition is 5 ~ 10%.
As a further improvement, the SM98011 protease, SM97010 protease preparation methods are as follows：
SM98011 strains and SM97010 strains are inoculated in the liquid fermentation medium of 500ml triangular flasks respectively, at 28 DEG C,
After 200rpm cultures 30h, zymotic fluid is centrifuged into 15min under 4 DEG C, 10000g, takes supernatant, as crude enzyme liquid；
The culture medium contains following weight fraction and compares component：1 ~ 3 part of corn flour, 0.5 ~ 2 part of wheat bran, 1 ~ 3 part of dregs of beans, oyster shell whiting
1 ~ 3 part, 0.4 ~ 0.6 part of disodium hydrogen phosphate, 0.03 ~ 0.05 part of potassium dihydrogen phosphate, 0.1 ~ 0.3 part of sodium carbonate, regulation pH is 7.5.
As a further improvement, the modification of chitosan preparation method is as follows：
（1）50 parts of chitosans are weighed, is added in the ethanol solutions of 600ml 95% and invades bubble, 60 DEG C of swelling 2h of constant temperature obtain chitosan
Invade bubble liquid；
（2）150 parts of vanillic aldehydes are dissolved in 400ml 95% ethanol, chitosan is poured into and invades bubble liquid, obtain mixed liquor；
（3）Mixed liquor is positioned in microwave quick reaction device, sets 70 DEG C of heating-up temperature, reacts 10min, microwave power
300w, stirring；
（4）Reaction is completed, and cooled and filtered, ethanol is washed, and is washed, is dried under vacuum to constant weight, you can obtains modification of chitosan；
The chitosan molecule amount is 5 ~ 60,000.
Chitosan after vanillic aldehyde graft modification has embedding ability and insoluble drug release ability well, in the soil of the present invention
In earth modifying agent, the materials such as more polypeptides, calcium activated and protein can be embedded, and control the rate of release of material, are ensured
Soil conditioner imitates continuous action.
As a further improvement, the high-pressure pulse electric be flow-type charging, by high-voltage pulse power source, oscillograph and
Manage room composition；The wave mode of the pulse power is triangular wave, impulse action caused by high-voltage pulse power source in the electrode of process chamber, so as to
The sample for flowing through process chamber is handled；Processing flow velocity is 0 ~ 20L/min.
High-voltage pulse electric field energy softens shell cuticula, is advantageous to enzymolysis and carries out faster.
Advantages of the present invention：
1st, shell calcium activated is extracted using microbial fermentation, active peptides are prepared into soil conditioner, beneficial bacterium in raising soil
Population and quantity, be effectively improved soil fertility；Active peptides can too high plant resistance against diseases, improve plant survival rate；
2nd, polyacrylamide and pitch breast are added, both act synergistically, and can more quickly and effectively play soil moisturizing effect, improve
The water content of soil, promote plant absorption nutriment；
3rd, containing micro needed for many plant growths in chicken manure, borax, zeolite powder, the dregs of a decoction, powder of straw, wood chip, paper mill sludge
Calcium activated and keratin synergy, promote plant absorption in element, with shell active material.
Embodiment
With reference to embodiment, the present invention is further described.
Embodiment 1
A. pretreatment of raw material
（1）Choose high-quality without the great shell to go mouldy, clean up, dry, be crushed to 200 mesh and obtain oyster shell whiting；
（2）Take 100 parts of oyster shell whitings with water according to solid-liquid ratio 1:10 is well mixed, then adds neutral proteinase, while be passed through height
Impulse electric field is pressed, setting electric-field intensity is 30Kv/cm, and umber of pulse 15, processing time 5min/h, 50 DEG C of heating water baths carry out enzyme
12h is solved, obtains enzymolysis liquid 1；The neutral protease vigor is 200,000 u/g, enzyme dosage 7%；The high-pressure pulse electric is stream
Dynamic formula feeds, and is made up of high-voltage pulse power source, oscillograph and process chamber；The wave mode of the pulse power is triangular wave, high-voltage pulse electric
Impulse action caused by source is in the electrode of process chamber, so as to handle the sample for flowing through process chamber；Handling flow velocity is
10L/min。
B. the preparation of active material
（1）Enzymolysis liquid 1 is centrifuged into 10min under 10000rpm, takes supernatant 1 standby, takes precipitation, add Portugal in mass ratio
Grape sugar 1.4%, corn extract 1.6%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.5%, regulation pH is 7, is put into fermentation tank, adds water
To the 60% of fermenter volume, after high pressure steam sterilization, compound bacteria is inoculated with, setting fermentation parameter is agitation revolution 200r/min, nothing
Bacterium air vent amount 1L/min, 37 DEG C of fermentation temperature, ferment 7 days, obtain zymotic fluid；
The compound bacteria inoculation method is：Bacillus licheniformis is first inoculated with, after fermenting 3 days, inoculating lactic acid bacterium, adds sucrose, after
Supervention ferment obtained zymotic fluid to 7 days；The bacillus licheniformis inoculum concentration is 7%, and lactobacillus inoculum amount is 5%, sucrose addition
For 5%；
（2）Zymotic fluid is taken, with lemon acid for adjusting pH to 6.5,10min is centrifuged under 5000rpm, takes supernatant 2 standby, takes
Precipitation, SM98011 protease, SM97010 protease 1 are added by its quality 8%:1 combination, in 50 DEG C, 130rpm shaking baths
5 ~ 6h is digested, obtains enzymolysis liquid 2；
C. the preparation of plant nutrition liquid
（1）Supernatant 1, supernatant 2, enzymolysis liquid 1 and enzymolysis liquid 2 are mixed, it is molten to add modification of chitosan by its ratio of weight and number
40 parts of liquid, 10 parts of humic acid, 10 parts of polyacrylamide, 10 parts of pitch breast, 20 parts of absolute ethyl alcohol, 1 part of calcium chloride powder, magnesium sulfate
1 part of powder, it is well mixed, 4h is then dried in 50 DEG C of blast driers, crosses 100 mesh sieves and powder is made；The modified shell gathers
Sugar is that the chitosan that molecular weight is 50,000 is carried out into vanillic aldehyde graft modification.
（2）Polyethylene glycol is dissolved at 70 DEG C, by polyethylene glycol, anhydrous citric acid powder and mercaptoethanol solution quality
Than for 2:1:1 is mixed, and crushes mixture after cooling, is crossed 80 mesh sieves and is obtained acid source；The mercaptoethanol solution concentration is
10~15mmol/L；
（3）Chicken manure, calcium superphosphate and borax are mixed, pH to 5 is adjusted after being down to room temperature, it is equal to be subsequently added into complex enzyme formulation stirring
It is even, zeolite powder, activated carbon are sprinkled into, heating is digested, then is warming up to 35 DEG C and is digested, enzymolysis time 3 days, then is warming up to
130 DEG C of inactivations obtain material 1；
The complex enzyme formulation is by cellulase and amylase according to 2:1 combines；
（4）After the dregs of a decoction, powder of straw, wood chip, paper mill sludge are mixed, crush, add polyvinyl alcohol and be uniformly mixing to obtain material
2；
（5）Powder, acid source, material 1, material 2 are well mixed, add 5% Macrogol 6000 solution, mixing in mass ratio
Uniformly, you can obtain plant nutrition liquid.
The plant nutrition liquid is used for sugarcane field, soil moisture evaporation reduces 20%, and beneficial bacterium quantity is 105Individual/cm3, pH
For 6.3, total nitrogen content 1.2g/kg, available phosphorus contents 2.61mg/kg, quick-acting potassium content 98.22mg/kg, sugarcane yield
Improve 16.4%.
Embodiment 2
A. pretreatment of raw material
（1）Choose high-quality without the great shell to go mouldy, clean up, dry, be crushed to 250 mesh and obtain oyster shell whiting；
（2）Take 100 parts of oyster shell whitings with water according to solid-liquid ratio 1:20 is well mixed, then adds neutral proteinase, while be passed through height
Impulse electric field is pressed, setting electric-field intensity is 35Kv/cm, and umber of pulse 20, processing time 10min/h, 60 DEG C of heating water baths are carried out
24h is digested, obtains enzymolysis liquid 1；The neutral protease vigor is 300,000 u/g, enzyme dosage 10%；The high-pressure pulse electric
Feed for flow-type, be made up of high-voltage pulse power source, oscillograph and process chamber；The wave mode of the pulse power is triangular wave, high-tension pulse
Impulse action caused by power supply is rushed in the electrode of process chamber, so as to handle the sample for flowing through process chamber；Handle flow velocity
For 20L/min.
B. the preparation of active peptides
（1）Enzymolysis liquid 1 is centrifuged into 15min under 10000rpm, takes supernatant 1 standby, takes precipitation, add Portugal in mass ratio
Grape sugar 2.0%, corn extract 2.0%, potassium dihydrogen phosphate 0.5%, sodium chloride 1%, regulation pH is 8, is put into fermentation tank, adds water to
The 70% of fermenter volume, after high pressure steam sterilization, compound bacteria is inoculated with, it is agitation revolution 200r/min to set fermentation parameter, sterile
Air vent amount 1.5L/min, 40 DEG C of fermentation temperature, ferment 10 days, obtain zymotic fluid；The compound bacteria is lichens gemma bar
Bacterium and lactic acid bacteria, inoculation method are：Bacillus licheniformis is first inoculated with, after fermenting 4 days, inoculating lactic acid bacterium, sucrose is added, continues to send out
Ferment obtained zymotic fluid to 10 days；The bacillus licheniformis inoculum concentration is 10%, and lactobacillus inoculum amount is 8%, and sucrose addition is
10%；
（2）Zymotic fluid is taken, with lemon acid for adjusting pH to 7.5,15min is centrifuged under 5000rpm, takes supernatant 2 standby；Take
Precipitation, SM98011 protease, alkali protease, SM97010 protease 1 are added by its quality 10%:2:1 combination, in 60 DEG C,
6h is digested in 130rpm shaking baths, obtains enzymolysis liquid 2；
C. the preparation of plant nutrition liquid
（1）Supernatant 1, supernatant 2, enzymolysis liquid 1 and enzymolysis liquid 2 are mixed, it is more molten than adding modification of chitosan by its weight fraction
50 parts of liquid, 15 parts of humic acid, 15 parts of polyacrylamide, 15 parts of pitch breast, 25 parts of absolute ethyl alcohol, 5 parts of calcium chloride powder, magnesium sulfate
5 parts of powder, it is well mixed, 6h is then dried in 60 DEG C of blast driers, crosses 100 mesh sieves and powder is made；The modified shell gathers
Sugar is that the chitosan that molecular weight is 60,000 is carried out into vanillic aldehyde graft modification.
（2）Polyethylene glycol is dissolved at 75 DEG C, by polyethylene glycol, anhydrous citric acid powder and mercaptoethanol solution quality
Than for 4:3:2 are mixed, and crush mixture after cooling, are crossed 80 mesh sieves and are obtained acid source；The mercaptoethanol solution concentration is
15mmol/L；
（3）Chicken manure, calcium superphosphate and borax are mixed, pH to 7 is adjusted after being down to room temperature, it is equal to be subsequently added into complex enzyme formulation stirring
It is even, zeolite powder, activated carbon are sprinkled into, heating is digested, then is warming up to 38 DEG C and is digested, enzymolysis time 6 days, then is warming up to
140 DEG C of inactivations obtain material 1；
The complex enzyme formulation is by cellulase and amylase according to 2:3 combine；
（4）After the dregs of a decoction, powder of straw, wood chip, paper mill sludge are mixed, crush, add polyvinyl alcohol and be uniformly mixing to obtain material
2；
（5）Powder, acid source, material 1, material 2 are well mixed, add 6% Macrogol 6000 solution, mixing in mass ratio
Uniformly, you can obtain plant nutrition liquid.
The plant nutrition liquid is used for acid wheatland, soil moisture evaporation reduces 22%, and beneficial bacterium quantity is 2 × 104
Individual/cm3, pH 6.5, total nitrogen content 1.01g/kg, available phosphorus contents 2.56mg/kg, quick-acting potassium content 97.04mg/
Kg, wheat yield improve 13.5%.
Embodiment 3
A. pretreatment of raw material
（1）Choose high-quality without the great shell to go mouldy, clean up, dry, be crushed to 220 mesh and obtain oyster shell whiting；
（2）Take 100 parts of oyster shell whitings with water according to solid-liquid ratio 1:15 is well mixed, then adds neutral proteinase, while be passed through height
Impulse electric field is pressed, setting electric-field intensity is 33Kv/cm, and umber of pulse 20, processing time 10min/h, 60 DEG C of heating water baths are carried out
24h is digested, obtains enzymolysis liquid 1；As a further improvement, the neutral protease vigor is 300,000 u/g, enzyme dosage 8%；Institute
State high-pressure pulse electric to feed for flow-type, be made up of high-voltage pulse power source, oscillograph and process chamber；The wave mode of the pulse power is
Triangular wave, impulse action caused by high-voltage pulse power source is in the electrode of process chamber, at the sample to flowing through process chamber
Reason；Processing flow velocity is 15L/min.
B. the preparation of active peptides
（1）Enzymolysis liquid 1 is centrifuged into 12min under 10000rpm, takes supernatant 1 standby, takes precipitation, add Portugal in mass ratio
Grape sugar 1.6%, corn extract 1.8%, potassium dihydrogen phosphate 0.4%, sodium chloride 0.8%, regulation pH is 7, is put into fermentation tank, adds water
To the 65% of fermenter volume, after high pressure steam sterilization, compound bacteria is inoculated with, setting fermentation parameter is agitation revolution 200r/min, nothing
Bacterium air vent amount 1.3L/min, 38 DEG C of fermentation temperature, ferment 8 days, obtain zymotic fluid；
The compound bacteria is bacillus licheniformis and lactic acid bacteria, and inoculation method is：Bacillus licheniformis is first inoculated with, is fermented 4 days
Afterwards, inoculating lactic acid bacterium, sucrose is added, continues fermentation to 8 days, obtain zymotic fluid；The bacillus licheniformis inoculum concentration is 8%, breast
Sour bacterium inoculum concentration is 6%, sucrose addition 8%；
（2）Zymotic fluid is taken, with lemon acid for adjusting pH to 7,15min is centrifuged under 5000rpm, takes supernatant 2 standby；It is heavy to take
Form sediment, SM98011 protease, alkali protease, SM97010 protease 1 are added by its quality 10%:2:1 combination, in 60 DEG C,
6h is digested in 130rpm shaking baths, obtains enzymolysis liquid 2；
C. the preparation of plant nutrition liquid
（1）Supernatant 1, supernatant 2, enzymolysis liquid 1 and enzymolysis liquid 2 are mixed, it is more molten than adding modification of chitosan by its weight fraction
45 parts of liquid, 13 parts of humic acid, 13 parts of polyacrylamide, 13 parts of pitch breast, 22 parts of absolute ethyl alcohol, 5 parts of calcium chloride powder, magnesium sulfate
5 parts of powder, it is well mixed, 6h is then dried in 60 DEG C of blast driers, crosses 100 mesh sieves and powder is made；
（2）Polyethylene glycol is dissolved at 70 DEG C, is by polyethylene glycol, anhydrous citric acid powder and mercaptoethanol solution quality ratio
3:1:2 are mixed, and crush mixture after cooling, are crossed 80 mesh sieves and are obtained acid source；The mercaptoethanol solution concentration is
10mmol/L；
（3）Chicken manure, calcium superphosphate and borax are mixed, pH to 6 is adjusted after being down to room temperature, it is equal to be subsequently added into complex enzyme formulation stirring
It is even, zeolite powder, activated carbon are sprinkled into, heating is digested, then is warming up to 35 ~ 38 DEG C and is digested, enzymolysis time 5 days, then is heated up
Material 1 is obtained to 140 DEG C of inactivations；
The complex enzyme formulation is by cellulase and amylase according to 1:1 combines；
（4）After the dregs of a decoction, powder of straw, wood chip, paper mill sludge are mixed, crush, add polyvinyl alcohol and be uniformly mixing to obtain material
2；
（5）Powder, acid source, material 1, material 2 are well mixed, add 6% Macrogol 6000 solution, mixing in mass ratio
Uniformly, you can obtain plant nutrition liquid.
The soil conditioner is used for acid milpa, soil moisture evaporation reduces 24%, and beneficial bacterium quantity is 105Individual/
cm3, pH 6.8, total nitrogen content 1.11g/kg, available phosphorus contents 2.14mg/kg, quick-acting potassium content 95.64mg/kg,
Corn yield improves 15.5%.
Comparative example
The plant nutrient solution and patent of invention CN201510616592.4 blueberry special nutritionals prepared using the embodiment of the present invention 1 ~ 3
Slow-release fertilizer to blueberry plantation apply fertilizer, the fertilising stage be blueberry plantation starts fertilising once, bloom fertilising once, result fertilising
Once.
Blueberry special nutritional slow-release fertilizer preparation method is as follows：
A kind of blueberry special nutritional slow-release fertilizer, its raw material include by weight：50 parts of paper mill sludge, 20 parts of earthworm manure, silkworm excrement
25 parts, 30 parts of pigeon dung, 30 parts of chicken manure, 10 parts of powder of straw, 26 parts of wood chip, 20 parts of sepiolite powder, 35 parts of activated carbon, 2 parts of effective microorganisms(EM),
60 parts of urea, 120 parts of biogas residue, 25 parts of potassium chloride, 8 parts of double superhosphate, 24 parts of sodium humate, 10 parts of modified montmorillonoid, lignite 7
Part, 1 part of Sodium Polyacrylate, 8 parts of acetate starch, 6 parts of kaolin, 18 parts of zeolite powder, 8 parts of amino acid/11.
Amino acid includes by weight：3.4 parts of asparatates, 5 parts of leucines, 3.8 parts of serines, 1.2 parts of threonines,
2.3 parts of glycine, 2.3 parts of proline.
The preparation technology of modified montmorillonoid is as follows：110 mesh sieves are crossed into montmorillonite, are warming up to 350 DEG C, it is cold after being incubated 2.6h
But modified montmorillonoid is obtained.
The preparation method for the above-mentioned blueberry special nutritional slow-release fertilizer that the present invention also proposes, comprises the following steps：
S1, after being well mixed paper mill sludge, earthworm manure, silkworm excrement, pigeon dung, chicken manure, powder of straw and wood chip, add sepiolite
After powder, activated carbon and effective microorganisms(EM) stir, compost fermentation 8d obtains fermentation materials, and every 1d stirs one in composting fermentation process
It is secondary；By fermentation materials low temperature drying, crush, add biogas residue and be well mixed, stand 2d and obtain first material；
S2, by Urea to 135 DEG C, after input potassium chloride, double superhosphate are well mixed, be once granulated, cooling,
Add sodium humate, modified montmorillonoid, lignite, Sodium Polyacrylate it is well mixed after, continuously add first material and stir
Afterwards, secondary granulation obtains second material；
S3, after acetate starch, kaolin, zeolite powder and amino acid are well mixed, add second material and be granulated three times,
Sieving obtains blueberry special nutritional slow-release fertilizer.
After Blueberry harvesting, to the plant nutrient solution and patent of invention prepared using embodiment 1 ~ 3
Soil parameters and blueberry yield after CN201510616592.4 blueberry special nutritional slow-release fertilizers are measured, and contrast number
According to such as table 1.
The soil items test parameter of table 1 and the contrast of blueberry yield data
By the data of table 1 it will be evident that can effectively improve soil fertility by the plant nutrition liquid of this bright preparation, crop is improved
Resistance against diseases, so as to improve crop yield.
The above embodiment of the present invention scheme is only the description of the invention and can not limit the present invention, in the power with the present invention
Any change in sharp claim suitable implication and scope, is all considered to be and is included within the scope of the claims.
Claims (7)
1. the preparation method of a plant nutrient liquor, it is characterised in that：Mainly prepared by following steps：
A. pretreatment of raw material
（1）Choose high-quality without the great shell to go mouldy, clean up, dry, be crushed to 200 ~ 250 mesh and obtain oyster shell whiting；
（2）Take 100 parts of oyster shell whitings with water according to solid-liquid ratio 1:(10 ~ 20) it is well mixed, then adds neutral proteinase, leads to simultaneously
Entering high-pressure pulse electric, setting electric-field intensity is 30 ~ 35Kv/cm, umber of pulse 15 ~ 20, and processing time is 5 ~ 10min/h, 50 ~ 60
DEG C heating water bath carries out 12 ~ 24h of enzymolysis, obtains enzymolysis liquid 1；
B. the preparation of active material
（1）Enzymolysis liquid 1 is centrifuged into 10 ~ 15min under 10000rpm, takes supernatant 1 standby, takes precipitation, is added in mass ratio
Enter glucose 1.4 ~ 2.0%, corn extract 1.6 ~ 2.0%, potassium dihydrogen phosphate 0.2 ~ 0.5%, sodium chloride 0.5 ~ 1%, regulation pH is 7
~ 8, it is put into fermentation tank, adds water to the 60 ~ 70% of fermenter volume, after high pressure steam sterilization, be inoculated with compound bacteria, fermentation ginseng is set
Number is 180 ~ 200r/min of agitation revolution, filtrated air 1 ~ 1.5L/min of throughput, 37 ~ 40 DEG C of fermentation temperature, is fermented 7 ~ 10 days,
Obtain zymotic fluid；
The compound bacteria is bacillus licheniformis and lactic acid bacteria；
（2）Zymotic fluid is taken, with lemon acid for adjusting pH to 6.5 ~ 7.5,10 ~ 15min is centrifuged under 5000rpm, takes supernatant 2
It is standby；Take precipitation, by its quality 8 ~ 10% add it is a kind of in SM98011 protease, alkali protease, SM97010 protease or
More than one combinations, 5 ~ 6h is digested in 50 ~ 60 DEG C, 130rpm shaking baths, obtains enzymolysis liquid 2；
C. the preparation of plant nutrition liquid
（1）Supernatant 1, supernatant 2, enzymolysis liquid 1 and enzymolysis liquid 2 are mixed, by its weight fraction than add chitosan solution 40 ~
50 parts, 10 ~ 15 parts of humic acid, 10 ~ 15 parts of polyacrylamide, 10 ~ 15 parts of pitch breast, 10 ~ 15 parts of peanut shell powder is well mixed, so
After drying 4 ~ 6h in 50 ~ 60 DEG C of blast driers, cross 100 mesh sieves and powder is made；
（2）Polyethylene glycol is dissolved at 70 ~ 75 DEG C, by polyethylene glycol, anhydrous citric acid powder and mercaptoethanol solution quality
Than for 2:1:1~4:3:2 are mixed, and crush mixture after then adding cooling, are crossed 80 mesh sieves and are obtained acid source；The sulfydryl
Ethanol solution concentration is 10 ~ 15mmol/L；
（3）Chicken manure, calcium superphosphate and borax are mixed, pH to 5 ~ 7 is adjusted after being down to room temperature, is subsequently added into complex enzyme formulation stirring
Uniformly, zeolite powder, activated carbon are sprinkled into, heating is digested, then is warming up to 35 ~ 38 DEG C and is digested, enzymolysis time 3 ~ 6 days, then
It is warming up to 130 ~ 140 DEG C of inactivations and obtains material 1；
The complex enzyme formulation is by cellulase and amylase according to 2:1~2:3 combine；
（4）After the dregs of a decoction, powder of straw, wood chip, paper mill sludge are mixed, crush, add polyvinyl alcohol and be uniformly mixing to obtain material
2；
（5）Powder, acid source, material 1, material 2 are well mixed, 5 ~ 6% Macrogol 6000 solution is added in mass ratio, mixes
Close uniform, you can obtain plant nutrition liquid.
2. the preparation method of a plant nutrient liquor according to claim 1, it is characterised in that the neutral protease
Power is 20 ~ 300,000 u/g, and enzyme dosage is 7 ~ 10%.
3. the preparation method of a plant nutrient liquor according to claim 1, it is characterised in that the compound bacteria inoculation side
Method is：Bacillus licheniformis is first inoculated with, after fermenting 3 ~ 4 days, inoculating lactic acid bacterium, adds sucrose, continues fermentation to 7 ~ 10 days, obtains
Zymotic fluid；The bacillus licheniformis inoculum concentration is 7 ~ 10%, and lactobacillus inoculum amount is 5 ~ 8%, and sucrose addition is 5 ~ 10%.
4. the preparation method of a plant nutrient liquor according to claim 1, it is characterised in that the SM98011 albumen
Enzyme, SM97010 protease preparation methods are as follows：
SM98011 strains and SM97010 strains are inoculated in the liquid fermentation medium of 500ml triangular flasks respectively, at 28 DEG C,
After 200rpm cultures 30h, zymotic fluid is centrifuged into 15min under 4 DEG C, 10000g, takes supernatant, as crude enzyme liquid；
The culture medium contains following weight fraction and compares component：1 ~ 3 part of corn flour, 0.5 ~ 2 part of wheat bran, 1 ~ 3 part of dregs of beans, oyster shell whiting
1 ~ 3 part, 0.4 ~ 0.6 part of disodium hydrogen phosphate, 0.03 ~ 0.05 part of potassium dihydrogen phosphate, 0.1 ~ 0.3 part of sodium carbonate, regulation pH is 7.5.
5. the preparation method of a plant nutrient liquor according to claim 1, it is characterised in that the modification of chitosan system
Preparation Method is as follows：
（1）50 parts of chitosans are weighed, is added in the ethanol solutions of 600ml 95% and invades bubble, 60 DEG C of swelling 2h of constant temperature obtain chitosan
Invade bubble liquid；
（2）150 parts of vanillic aldehydes are dissolved in 400ml 95% ethanol, chitosan is poured into and invades bubble liquid, obtain mixed liquor；
（3）Mixed liquor is positioned in microwave quick reaction device, sets 70 DEG C of heating-up temperature, reacts 10min, microwave power
300w, stirring；
（4）Reaction is completed, and cooled and filtered, ethanol is washed, and is washed, is dried under vacuum to constant weight, you can obtains modification of chitosan；
The chitosan molecule amount is 5 ~ 60,000.
6. the preparation method of a plant nutrient liquor according to claim 1, it is characterised in that：The high-pressure pulse electric
Feed for flow-type, be made up of high-voltage pulse power source, oscillograph and process chamber；The wave mode of the pulse power is triangular wave, high-tension pulse
Impulse action caused by power supply is rushed in the electrode of process chamber, so as to handle the sample for flowing through process chamber；Handle flow velocity
For 0 ~ 20L/min.
7. the preparation method of a plant nutrient liquor according to claim 1, it is characterised in that the SM98011 albumen
Enzyme, alkali protease, SM97010 protease additions ratio are 1:2:1.
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| CN109251108A (en) * | 2018-11-28 | 2019-01-22 | 廖艺 | One plant nutrient liquor and its preparation and method of administration |
| CN113666786A (en) * | 2021-08-13 | 2021-11-19 | 广东拉多美化肥有限公司 | Oyster shell activation method, mixed solution, oyster shell composition and preparation method of soil conditioner |
| CN114680084A (en) * | 2020-12-29 | 2022-07-01 | 肥西吉新蚕桑专业合作社 | Breeding method for improving activity and health of young silkworms |
| CN117417061A (en) * | 2023-12-19 | 2024-01-19 | 临沂市环境保护科学研究所有限公司 | Composite treating agent for high-concentration ammonia nitrogen sewage and preparation method thereof |
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