CN105483057A - Functional microbial preparation and preparation method thereof - Google Patents
Functional microbial preparation and preparation method thereof Download PDFInfo
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- CN105483057A CN105483057A CN201610022028.4A CN201610022028A CN105483057A CN 105483057 A CN105483057 A CN 105483057A CN 201610022028 A CN201610022028 A CN 201610022028A CN 105483057 A CN105483057 A CN 105483057A
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- chicken manure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
Abstract
The invention discloses a functional microbial preparation which can shorten the fermentation time of chicken manure and increase the utilization rate of the chicken manure. The functional microbial preparation comprises components by weight as follows: 3-8 parts of Saccharomyces cerevisiae, 3-7 parts of enterococcus faecalis, 4-6 parts of Aspergillus oryzae, 3-5 parts of bacillus coagulans, 3-4 parts of Bacillus mucilaginosus and 2-5 parts of bacillus licheniformis. The invention further provides the functional microbial preparation which can shorten the fermentation time of chicken manure and increase the utilization rate of the chicken manure and a preparation method. The functional microbial preparation and the preparation method have the advantages as follows: the functional microbial preparation which can shorten the fermentation time of chicken manure and increase the utilization rate of the chicken manure contains multiple microorganisms, the fermentation time of chicken manure can be shortened, loss of nutritional ingredients in the chicken manure is reduced, the utilization rate of the chicken manure is increased, rapid release of fertilizers in the chicken manure is promoted, germs in the chicken manure are rapidly killed, crop absorption is facilitated, and health growth of crops is promoted.
Description
Technical field:
The present invention relates to a kind of biotechnological formulation and preparation method, particularly relate to a kind of functional microbial preparation and preparation method.
Background technology:
Chicken manure is a kind of fertilizer comparing high-quality, and it is about 1.63%, 1.54%, 0.85% containing purity nitrogen, phosphorus (P2O5), potassium (K2O).Essential substance in chicken manure is organic, uses chicken manure and adds organic content in soil.Organic matter can improve soil physics, chemistry and biological nature, vegetable soil, culture fertility.China rural area " support by excrement, seedling is long by excrement " proverb, reflect to a certain extent and use the effect of chicken manure material for improvement soil.Use chicken manure fertilizer and both add many organic colloids, effect simultaneously by microorganism becomes organic colloid many organism also decomposition and inversion, this just considerably increases adsorption by soil surface, and produce many gummous substances, make that soil particle is cementing becomes stable crumb structure, improve soil water-retaining, fertilizer conservation and ventilative performance, and regulate the ability of the soil moisture.But chicken manure has to pass through before administration and becomes thoroughly decomposed fully, by the parasite that exists in chicken manure and ovum thereof, and more communicable germ is by obtaining deactivation in the process of become thoroughly decomposed (retting).Because chicken manure produces high temperature in the process of becoming thoroughly decomposed, easily cause nitrogen loss.Therefore, will add water in right amount before becoming thoroughly decomposed, and add the calcium superphosphate of 5%, fertilizer efficiency can be better.Chicken manure can be dropped into manure pit bubble and macerate by the method for becoming thoroughly decomposed, and also can carry out surperficial grave mound stack retting.Chicken manure becomes the high-quality base manure of long-term cropping after fully becoming thoroughly decomposed, or Chang Zuowei executes the base manure utilized the lower whole year winter in raising fruit trees.Traditional method is that chicken manure is sealed stack retting, and carry out anaerobically fermenting, fermentation time is long, can cause a large amount of nutrient component damages; Fermentation time is too short, and the germ in chicken manure is killed not thorough, brings disease hidden danger to crop.
Summary of the invention:
In view of this, the invention provides a kind of functional microbial preparation, it has containing multiple-microorganism, can shorten the chicken manure fermenting time, improves chicken manure utilization ratio, kills the germ in chicken manure fast.
The present invention also provides a kind of preparation method of functional microbial preparation.
For solving above technical problem, technical scheme of the present invention is: a kind of functional microbial preparation, its moiety, calculate by weight, comprising: yeast saccharomyces cerevisiae 3-8 part, enterococcus faecalis 3-7 part, aspergillus oryzae 4-6 part, Bacillus coagulans 3-5 part, colloid bacillus cereus 3-4 part, Bacillus licheniformis 2-5 part.
Preferably, functional microbial preparation of the present invention comprises the material of following parts by weight: yeast saccharomyces cerevisiae 8 parts, enterococcus faecalis 7 parts, aspergillus oryzae 6 parts, Bacillus coagulans 4 parts, colloid bacillus cereus 4 parts, Bacillus licheniformis 5 parts.
Preferably, functional microbial preparation of the present invention comprises the material of following parts by weight: yeast saccharomyces cerevisiae 7 parts, enterococcus faecalis 6 parts, aspergillus oryzae 5 parts, Bacillus coagulans 4 parts, colloid bacillus cereus 3 parts, Bacillus licheniformis 3 parts.
Preferably, functional microbial preparation of the present invention comprises the material of following parts by weight: yeast saccharomyces cerevisiae 6 parts, enterococcus faecalis 5 parts, aspergillus oryzae 6 parts, Bacillus coagulans 4 parts, colloid bacillus cereus 4 parts, Bacillus licheniformis 3 parts.
A preparation method for functional microbial preparation, comprises the following steps:
1) choose six culture tank and carry out sterilising treatment;
2) add nutrient solution respectively in the culture tank of sterilising treatment rear end, the volume of nutrient solution is no more than 2/3rds of culture tank volume;
3) get appropriate yeast saccharomyces cerevisiae, enterococcus faecalis, aspergillus oryzae, Bacillus coagulans, colloid bacillus cereus, Bacillus licheniformis to put into the culture tank after sterilising treatment respectively and carry out monomer cultivation;
4) after maturation, yeast saccharomyces cerevisiae 6 parts got by meter by weight, enterococcus faecalis 5 parts, aspergillus oryzae 6 parts add the culture tank that mixed-culture medium is housed and carry out mixed culture, and culture tank temperature, at 26-38 DEG C, cultivates 2-3 days;
5) Bacillus coagulans 3-5 part got by meter by weight, colloid bacillus cereus 3-4 part, Bacillus licheniformis 2-5 part, aspergillus oryzae 1-2 part adds in mixed culture tank, and stir i.e. obtained product.
The mixed-culture medium of described step 4 is mixed by the material of following parts by weight: pure water 50-60 part, glucose 3-5 part, Fructus Hordei Germinatus powder 5-8 part, soybean cake powder 2-3 part.
Compared with prior art, tool of the present invention has the following advantages: this kind of functional microbial preparation provided by the present invention, it has containing multiple-microorganism, the chicken manure fermenting time can be shortened, reduce the loss of chicken manure Middle nutrition composition, improve chicken manure utilization ratio, promote the quick release of fertilizer efficiency in chicken manure, kill the germ in chicken manure fast, be convenient to the absorption of crop, promote healthy growth of crops.
Embodiment
In order to make those skilled in the art understand technical scheme of the present invention better, below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1
A kind of functional microbial preparation, its moiety, calculates by weight, and comprising: yeast saccharomyces cerevisiae 8 parts, enterococcus faecalis 7 parts, aspergillus oryzae 6 parts, Bacillus coagulans 4 parts, colloid bacillus cereus 4 parts, Bacillus licheniformis 5 parts.
The preparation method of embodiment 1 comprises the following steps:
1) choose six culture tank and carry out sterilising treatment;
2) add nutrient solution respectively in the culture tank of sterilising treatment rear end, the volume of nutrient solution is no more than 2/3rds of culture tank volume;
3) get appropriate yeast saccharomyces cerevisiae, enterococcus faecalis, aspergillus oryzae, Bacillus coagulans, colloid bacillus cereus, Bacillus licheniformis to put into the culture tank after sterilising treatment respectively and carry out monomer cultivation;
4) after maturation, yeast saccharomyces cerevisiae 8 parts got by meter by weight, enterococcus faecalis 7 parts, aspergillus oryzae 6 parts add the culture tank that mixed-culture medium is housed and carry out mixed culture, and culture tank temperature, at 26-38 DEG C, cultivates 2-3 days;
5) Bacillus coagulans 4 parts got by meter by weight, colloid bacillus cereus 4 parts, Bacillus licheniformis 5 parts add in mixed culture tank, and stir i.e. obtained product.
Embodiment 2
A kind of functional microbial preparation, its moiety, calculates by weight, and comprising: yeast saccharomyces cerevisiae 7 parts, enterococcus faecalis 6 parts, aspergillus oryzae 5 parts, Bacillus coagulans 4 parts, colloid bacillus cereus 3 parts, Bacillus licheniformis 3 parts.
The preparation method of embodiment 2 comprises the following steps:
1) choose six culture tank and carry out sterilising treatment;
2) add nutrient solution respectively in the culture tank of sterilising treatment rear end, the volume of nutrient solution is no more than 2/3rds of culture tank volume;
3) get appropriate yeast saccharomyces cerevisiae, enterococcus faecalis, aspergillus oryzae, Bacillus coagulans, colloid bacillus cereus, Bacillus licheniformis to put into the culture tank after sterilising treatment respectively and carry out monomer cultivation;
4) after maturation, yeast saccharomyces cerevisiae 7 parts got by meter by weight, enterococcus faecalis 6 parts, aspergillus oryzae 5 parts add the culture tank that mixed-culture medium is housed and carry out mixed culture, and culture tank temperature, at 26-38 DEG C, cultivates 2-3 days;
5) Bacillus coagulans 4 parts got by meter by weight, colloid bacillus cereus 3 parts, Bacillus licheniformis 3 parts add in mixed culture tank, and stir i.e. obtained product.
Embodiment 3
A kind of functional microbial preparation, its moiety, calculates by weight, and comprising: yeast saccharomyces cerevisiae 6 parts, enterococcus faecalis 5 parts, aspergillus oryzae 6 parts, Bacillus coagulans 4 parts, colloid bacillus cereus 4 parts, Bacillus licheniformis 3 parts.
The preparation method of embodiment 3 comprises the following steps:
1) choose six culture tank and carry out sterilising treatment;
2) add nutrient solution respectively in the culture tank of sterilising treatment rear end, the volume of nutrient solution is no more than 2/3rds of culture tank volume;
3) get appropriate yeast saccharomyces cerevisiae, enterococcus faecalis, aspergillus oryzae, Bacillus coagulans, colloid bacillus cereus, Bacillus licheniformis to put into the culture tank after sterilising treatment respectively and carry out monomer cultivation;
4) after maturation, yeast saccharomyces cerevisiae 6 parts got by meter by weight, enterococcus faecalis 5 parts, aspergillus oryzae 6 parts add the culture tank that mixed-culture medium is housed and carry out mixed culture, and culture tank temperature, at 26-38 DEG C, cultivates 2-3 days;
5) Bacillus coagulans 4 parts got by meter by weight, colloid bacillus cereus 4 parts, Bacillus licheniformis 3 parts add in mixed culture tank, and stir i.e. obtained product.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred implementation should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, some improvements and modifications can also be made and also should be considered as protection scope of the present invention.
Claims (3)
1. a functional microbial preparation, it is characterized in that, its moiety, calculates by weight, and comprising: yeast saccharomyces cerevisiae 3-8 part, enterococcus faecalis 3-7 part, aspergillus oryzae 4-6 part, Bacillus coagulans 3-5 part, colloid bacillus cereus 3-4 part, Bacillus licheniformis 2-5 part.
2. a preparation method for functional microbial preparation, is characterized in that, comprises the following steps:
1) choose six culture tank and carry out sterilising treatment;
2) add nutrient solution respectively in the culture tank of sterilising treatment rear end, the volume of nutrient solution is no more than 2/3rds of culture tank volume;
3) get appropriate yeast saccharomyces cerevisiae, enterococcus faecalis, aspergillus oryzae, Bacillus coagulans, colloid bacillus cereus, Bacillus licheniformis to put into the culture tank after sterilising treatment respectively and carry out monomer cultivation;
4) after maturation, yeast saccharomyces cerevisiae 6 parts got by meter by weight, enterococcus faecalis 5 parts, aspergillus oryzae 6 parts add the culture tank that mixed-culture medium is housed and carry out mixed culture, and culture tank temperature, at 26-38 DEG C, cultivates 2-3 days;
5) Bacillus coagulans 3-5 part got by meter by weight, colloid bacillus cereus 3-4 part, Bacillus licheniformis 2-5 part, aspergillus oryzae 1-2 part adds in mixed culture tank, and stir i.e. obtained product.
3. the preparation method of described a kind of functional microbial preparation according to claim 2, it is characterized in that, mixed-culture medium described in step 4 is mixed by the material of following parts by weight: pure water 50-60 part, glucose 3-5 part, Fructus Hordei Germinatus powder 5-8 part, soybean cake powder 2-3 part.
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| CN201610022028.4A CN105483057A (en) | 2016-01-07 | 2016-01-07 | Functional microbial preparation and preparation method thereof |
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| CN201610022028.4A CN105483057A (en) | 2016-01-07 | 2016-01-07 | Functional microbial preparation and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047769A (en) * | 2016-08-04 | 2016-10-26 | 北京好实沃生物技术有限公司 | Bacillus coagulans-containing composite inocula and preparation method thereof |
| CN107099494A (en) * | 2017-07-07 | 2017-08-29 | 湖北华扬科技发展有限公司 | A kind of livestock manure fermented microbial inoculum and its application process |
| CN107099493A (en) * | 2017-07-06 | 2017-08-29 | 裴德修 | Compound microbial excrement decomposing agent and preparation method and excrement become thoroughly decomposed processing method |
| CN113462603A (en) * | 2021-07-11 | 2021-10-01 | 菏泽嘉麟生物科技有限公司 | Preparation method and application of composite fecal sewage treatment microbial inoculum |
Citations (4)
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| CN101665773A (en) * | 2008-12-23 | 2010-03-10 | 中国农业科学院农业环境与可持续发展研究所 | Microbial bacterial agent, preparation method thereof and method for treating animal excrements |
| CN101935236A (en) * | 2010-09-27 | 2011-01-05 | 上海创博生态工程有限公司 | Method for preparing acid organic fertilizer by orientation fermentation of organic waste |
| CN104193431A (en) * | 2014-09-05 | 2014-12-10 | 广西三堡农业科技有限公司 | Special complex microbial agent for harmless treatment of livestock and poultry excrements, and preparation method |
| CN104232551A (en) * | 2014-09-29 | 2014-12-24 | 山东苏柯汉生物工程股份有限公司 | Pig-chicken excrement fermenting compound bacteria, preparation method and method of utilizing compound bacteria to ferment and prepare organic fertilizer |
-
2016
- 2016-01-07 CN CN201610022028.4A patent/CN105483057A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101665773A (en) * | 2008-12-23 | 2010-03-10 | 中国农业科学院农业环境与可持续发展研究所 | Microbial bacterial agent, preparation method thereof and method for treating animal excrements |
| CN101935236A (en) * | 2010-09-27 | 2011-01-05 | 上海创博生态工程有限公司 | Method for preparing acid organic fertilizer by orientation fermentation of organic waste |
| CN104193431A (en) * | 2014-09-05 | 2014-12-10 | 广西三堡农业科技有限公司 | Special complex microbial agent for harmless treatment of livestock and poultry excrements, and preparation method |
| CN104232551A (en) * | 2014-09-29 | 2014-12-24 | 山东苏柯汉生物工程股份有限公司 | Pig-chicken excrement fermenting compound bacteria, preparation method and method of utilizing compound bacteria to ferment and prepare organic fertilizer |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047769A (en) * | 2016-08-04 | 2016-10-26 | 北京好实沃生物技术有限公司 | Bacillus coagulans-containing composite inocula and preparation method thereof |
| CN106047769B (en) * | 2016-08-04 | 2020-01-31 | 北京好实沃生物技术有限公司 | composite microbial inoculum containing bacillus coagulans and preparation method thereof |
| CN107099493A (en) * | 2017-07-06 | 2017-08-29 | 裴德修 | Compound microbial excrement decomposing agent and preparation method and excrement become thoroughly decomposed processing method |
| CN107099494A (en) * | 2017-07-07 | 2017-08-29 | 湖北华扬科技发展有限公司 | A kind of livestock manure fermented microbial inoculum and its application process |
| CN113462603A (en) * | 2021-07-11 | 2021-10-01 | 菏泽嘉麟生物科技有限公司 | Preparation method and application of composite fecal sewage treatment microbial inoculum |
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Application publication date: 20160413 |