CN109180261A - A kind of vegetables selenium-rich liquid bacterial manure and the preparation method and application thereof - Google Patents
A kind of vegetables selenium-rich liquid bacterial manure and the preparation method and application thereof Download PDFInfo
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- CN109180261A CN109180261A CN201811091652.5A CN201811091652A CN109180261A CN 109180261 A CN109180261 A CN 109180261A CN 201811091652 A CN201811091652 A CN 201811091652A CN 109180261 A CN109180261 A CN 109180261A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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Abstract
The invention discloses a kind of vegetables selenium-rich liquid bacterial manure and preparation method thereof, 8-15 containing nanometer selenium parts, 5-15 parts of Bacillus cercus microbial inoculum, 5-15 parts of germ oligotrophy unit cell microbial inoculum, 5-12 parts of chitosan, EDTA4-8 parts, 8-15 parts of amino acid, micro- 5-15 parts of fertilizer, 45-60 parts of water.Applied to the yield for improving vegetables selenium content, increase vegetables and improve quality of vegetable.Have many advantages, such as that significant effect, green non-pollution, utilization rate of fertilizer are high, improve yield and quality, is low in cost and easy to spread.
Description
Technical field
The present invention relates to vegetable growing technologies and fertilising field, and in particular to a kind of vegetables selenium-rich liquid bacterial manure and its preparation
Method and application.
Background technique
Selenium is one of the essential trace elements of the human body, and selenium participates in forming glutathione peroxidase, has anticancer, prevents
Cancer, cardioprotection and anti-aging, it is anti-oxidant with eliminate free radical, a variety of special healthcare functions such as improve the immunity of the human body.People
Intake selenium approach guarantee mainly by the food containing selenium, Chinese Soclety of Nutrition's special recommendation China resident selenium intake be 50~
200 μ g/ days, and selenium deficiency Area Inhabitants current selenium intake in China's is only 40~50 μ g/ days, is good for so human body selenium-supply diseases prevention improves
Kang Shuiping, optimal path are scientific tonic selenium-rich agricultural and sideline products.
The nutriment of vegetables mainly includes minerals, vitamin, fiber etc., and the content of these substances is higher, vegetables
Nutritive value is also higher.It is estimated that there is more than 20 hundred million or more people to be caused a variety of diseases by environmental pollution in the world now,
How to solve the problems, such as that a large amount of oxygen radicals are generated because of environmental pollution is increasingly subject to people's concern.One of effective way of solution,
It is to increase antioxidant collaboration in food to remove excessive damaging active oxygen, active nitrogen.The study found that having perhaps in vegetables
Multivitamin, mineral trace element and relevant phytochemicals, enzyme etc. are all effective antioxidants, so vegetables are not
It is only the health food of low sugar, less salt, low fat, while can also effectively mitigates damage of the environmental pollution to human body, while vegetables
Prevention effect also is played to various diseases.
The specific function and selenium element of Se-rich farm products are the necessary mineral trace elements of human body, develop rich selenium vegetables pair
It has important practical significance and strategic importance in the sustainable development of vegetables industry.Common selenium-enriched fertilizer master currently on the market
If the mixing of selenium mineral powder and conventional chemical fertilizers, since natural selenium element is with the presence of inorganic compound state, it is not easy to plant
Sorption enhanced, so be easy to causeing the waste and environmental pollution of resource.Present invention seek to address that prior art problem and provide
A kind of vegetables selenium-rich liquid bacterial manure, the selenium-rich liquid bacterial manure are the life by Bacillus cercus and germ oligotrophy unit cell
Nanometer selenium is converted organic selenium, while the nutriments such as EDTA and organic selenium, chitosan, amino acid and micro- fertilizer by metabolic activity
Chelating, enable vegetable leaf preferably absorption and accumulation selenium element.
Summary of the invention
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, meets vegetables at significant effect
The vegetables selenium-rich liquid bacterial manure of growing nutrient demand.
Technical scheme is as follows:
A kind of vegetables selenium-rich liquid bacterial manure, it is made from the following raw materials in parts by weight: 8-15 parts of nanometer selenium, wax-like gemma bar
5-15 parts of bacteria agent, 5-15 parts of germ oligotrophy unit cell microbial inoculum, 5-12 parts of chitosan, EDTA4-8 parts, 8-15 parts of amino acid,
Micro- 5-15 parts of fertilizer, 45-60 parts of water.Signified vegetables include leaf vegetables, melon-fruit-like vegetable, stem vegetables, flower vegetables,
Root vegetable.
Preferably, it is made from the following raw materials in parts by weight: 10 parts of nanometer selenium, 12 parts of Bacillus cercus microbial inoculum, thermophilic wheat
12 parts of bud Stenotrophomonas microbial inoculum, 8 parts of chitosan, EDTA6 parts, it is 0 part of amino acid 1, fertile 10 parts, 32 parts of water micro-.
Wherein, the strain contained in said waxy gemma bacillus agent be Bacillus cercus LY05 (CCTCCNO:
M2015397), Bacillus cercus LGY06 (CCTCCNO:M2015398) and Bacillus cercus WL08 (CCTCCNO:
M2015399 a combination of one or more)；The strain of the germ oligotrophy unit cell microbial inoculum is thermophilic malt oligotrophy list
Born of the same parents bacterium XQ08 (CCTCCNO:M2015726).The preparation of two kinds of microbial inoculums includes the following steps: that bacterial strain inclined-plane is saved test tube by (1)
Seed is seeded in minimal medium respectively, shaken cultivation to logarithmic growth phase, obtains bacterium solution；(2) by the bacterium solution of acquisition with
The inoculum concentration of 10% percent by volume is seeded in the seed fermentation tank containing minimal medium, culture to logarithmic growth
Phase obtains seed liquor；(3) seed liquor of acquisition is inoculated in fermentation medium according to the inoculum concentration of 10% volume ratio,
30-35 DEG C, fermented and cultured 2-3 days under 150-200rpm, obtain fermentation liquid, the living bacteria count of fermentation liquid >=10,000,000,000/mL,
Fermentation liquid is directly diluted 5-20 times respectively and obtains Bacillus cercus microbial inoculum, germ oligotrophy unit cell liquid bacterial agent.
Further, the chitosan is water solubility, and deacetylation is greater than 95%, and molecular weight is 10~50KDa；The ammonia
Base acid is proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, color ammonia
Acid, histidine, tyrosine, valine, alanine, serine, arginine, asparatate, any five kinds in cysteine
Or more combination；The EDTA is disodium ethylene diamine tetraacetate；Micro- fertilizer is mass ratio are as follows: 3-6:2-5:1-2:1-2:1-
Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer of 2:0.5-1:0.5-1:0.5-1；The water is
Sterile water.
The preparation method of vegetables selenium-rich liquid bacterial manure of the invention, comprises the technical steps that:
Step 1. takes obtained Bacillus cercus microbial inoculum, germ oligotrophy unit cell liquid bacterial agent by regulation proportion, standby
With；
Step 2. is taken nanometer selenium, chitosan, EDTA, amino acid and micro- fertilizer to be sufficiently stirred to be dissolved in required proportion by regulation proportion
Sterile water in；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, pH6.0~6.8
Under conditions of, it is sufficiently mixed in sterile blender uniformly, it is packed up to vegetables selenium-rich liquid bacterial fertilizer products.
Compared with prior art, the present invention selenium-rich liquid bacterial manure of the invention is few by Bacillus cercus and thermophilic malt
The vital metabolic activity for supporting monad, converts organic selenium for nanometer selenium, at the same EDTA and organic selenium, chitosan, amino acid and
The chelating of the nutriments such as micro- fertilizer, enables vegetable leaf preferably absorption and accumulation selenium element.With significant effect, green without dirt
Dye, the advantages that utilization rate of fertilizer is high, low in cost and easy to spread.
Specific embodiment
Below with reference to embodiment, the invention will be further described, but not as any limitation of the invention.In this hair
Numerical value or number ratios involved in bright indicate mass figures or mass ratio unless otherwise instructed.
Embodiment 1:
1. selenium-rich liquid bacterial manure composition and preparation:
Selenium-rich liquid bacterial manure formula composition and preparation, (1) composition: 10 parts of nanometer selenium, 12 parts of Bacillus cercus microbial inoculum, thermophilic
12 parts of malt Stenotrophomonas microbial inoculum, 8 parts of chitosan, EDTA6 parts, it is 0 part of amino acid 1, fertile 10 parts, 32 parts of water micro-.(2) it prepares:
1. Bacillus cercus microbial inoculum and germ oligotrophy unit cell liquid bacterial agent are taken by regulation proportion, it is spare；2. being taken by regulation proportion
Nanometer selenium, chitosan, EDTA, amino acid and micro- fertilizer, which are sufficiently stirred, to be dissolved in the required sterile water matched；3. 1. step is obtained
Microbial inoculum be added in the solution that 2. obtains of step, under conditions of 25-30 DEG C of temperature, pH6.0-6.8, filled in sterile blender
Divide and is uniformly mixed, it is packed up to vegetables selenium-rich liquid bacterial manure.
2. field trial:
Experimental field overview: being experimental field located at Guizhou Xifeng County Western Hills township herbary, and kind is tomato " drawing 118 in red pool ".
It is yellow earth for examination soil types, random in vegetable garden before test, multiple spot mixing acquisition depth 0-60cm soil sample is as Soil Background
Value, pH value 5.86, total nitrogen content 1.74gkg-1, content of organic matter 39.53gkg-1, alkaline hydrolysis nitrogen content is
108.76mg·kg-1, available phosphorus content 47.63mgkg-1, effective potassium content is 103.47mgkg-1。
Field experimental design: RANDOMIZED BLOCK DESIGN is taken in test, using complete stool (including blade face front and back sides, limb, fruit
Deng) spraying, if 200 times of liquid of selenium-rich liquid bacterial manure, spraying clear water as control (CK), totally 2 processing are repeated 4 times, 8 cells totally,
Surrounding sets protection row.Administration time is to apply respectively seedling stage, growth medium, prosperous growth phase, and amount of application is respectively 30,45,60kg/
Mu.Weather is based on fine during test, or it is cloudy, spray in latter 3-4 days without rainfall.
3. analysis project and method:
Investigate yield, quality survey dry matter, vitamin C, titratable acid, soluble solid, lycopene.Se content
It is measured using National Standard Method GB/5009.93-2010.
4. embodiment effect:
Increase from table 1 to table 2 as can be seen that tomato per mu yield can be effectively facilitated using vegetables selenium-rich liquid bacterial manure,
Fruit dry matter, vitamin C and soluble solid (SSC) content are significantly improved, titratable acid (TA) content is reduced, promotes vegetable
The formation of dish yield and the improvement of quality；And can effectively vegetables fruit organic selenium, inorganic selenium and total Se content, and can improve organic
The accounting of selenium is more advantageous to people and ingests to selenium.
Table 1 applies influence of the selenium-rich liquid bacterial manure to tomato production and quality
Influence of the 2 selenium-rich liquid bacterial manure of table to tomato selenium content
It is above specific application example of the invention, there are other embodiments of the invention, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection scope of the presently claimed invention.
Claims (5)
1. a kind of vegetables selenium-rich liquid bacterial manure, it is characterised in that: it is made from the following raw materials in parts by weight: 8-15 parts of nanometer selenium,
5-15 parts of Bacillus cercus microbial inoculum, 5-15 parts of germ oligotrophy unit cell microbial inoculum, 5-12 parts of chitosan, 4-8 parts of EDTA, ammonia
8-15 parts of base acid, micro- 5-15 parts of fertilizer, 45-60 parts of water；The vegetables include leaf vegetables, melon-fruit-like vegetable, stem vegetables,
Flower vegetables, root vegetable.
2. vegetables selenium-rich liquid bacterial manure according to claim 1, it is characterised in that: it by following parts by weight raw material system
At: 10 parts of nanometer selenium, 12 parts of Bacillus cercus microbial inoculum, 12 parts of germ oligotrophy unit cell microbial inoculum, 8 parts of chitosan, EDTA 6
It is part, 0 part of amino acid 1, fertile 10 parts, 32 parts of water micro-.
3. vegetables selenium-rich liquid bacterial manure according to claim 1 or 2, it is characterised in that: said waxy gemma bacillus agent
In the strain that contains be Bacillus cercus LY05, one kind of Bacillus cercus LGY06 and Bacillus cercus WL08 or two
Kind or more combination；The strain of the germ oligotrophy unit cell microbial inoculum is germ oligotrophy unit cell XQ08；Two kinds of microbial inoculums
Preparation includes the following steps: that bacterial strain inclined-plane is saved test tube seed by (1), is seeded in minimal medium respectively, oscillation training
It supports to logarithmic growth phase, obtains bacterium solution；(2) by the bacterium solution of acquisition with the inoculum concentration of 10% percent by volume be seeded to containing whether there is or not
In the seed fermentation tank of machine salt culture medium, culture to logarithmic growth phase obtains seed liquor；(3) by the seed liquor of acquisition according to
The inoculum concentration of 10% volume ratio is inoculated in fermentation medium, and fermented and cultured 2-3 days, is obtained at 30-35 DEG C, 150-200rpm
Fermentation liquid, the living bacteria count of fermentation liquid >=10,000,000,000/mL, fermentation liquid is directly diluted respectively 5-20 times obtain it is wax-like
Gemma bacillus agent, germ oligotrophy unit cell liquid bacterial agent.
4. vegetables selenium-rich liquid bacterial manure according to claim 1 or 2, it is characterised in that: the chitosan is water solubility, is taken off
Acetyl degree is greater than 95%, and molecular weight is 10~50KDa；The amino acid is proline, glycine, glutamic acid, phenylalanine, bright
Propylhomoserin, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine,
Arginine, asparatate, any five kinds or more of combination in cysteine；The EDTA is ethylenediamine tetra-acetic acid two
Sodium；Micro- fertilizer is mass ratio are as follows: potash fertilizer+calcareous fertilisers+boron fertilizer of 3-6:2-5:1-2:1-2:1-2:0.5-1:0.5-1:0.5-1+
Fertiliser containing magnesium+iron fertilizer+zinc fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer；The water is sterile water.
5. a kind of preparation method of vegetables selenium-rich liquid bacterial manure as claimed in claim 1 or 2, it is characterised in that: including as follows
Processing step:
Step 1. takes obtained Bacillus cercus microbial inoculum, germ oligotrophy unit cell liquid bacterial agent, spare；
Step 2. takes nanometer selenium, chitosan, EDTA, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportion；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., the item in 25-30 DEG C of temperature, pH6.0~6.8
Under part, it is sufficiently mixed in sterile blender uniformly, it is packed up to vegetables selenium-rich liquid bacterial fertilizer products.
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| CN201811091652.5A CN109180261A (en) | 2018-09-19 | 2018-09-19 | A kind of vegetables selenium-rich liquid bacterial manure and the preparation method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111763112A (en) * | 2019-03-29 | 2020-10-13 | 张德珍 | Biological amino acid-integrated fruit, vegetable and grain leaf surface spraying agent containing nano-selenium |
| CN115707675A (en) * | 2022-10-17 | 2023-02-21 | 镇江丰泰农业科技有限公司 | Special selenium-rich and zinc-rich functional fertilizer for rice |
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| CN115707675A (en) * | 2022-10-17 | 2023-02-21 | 镇江丰泰农业科技有限公司 | Special selenium-rich and zinc-rich functional fertilizer for rice |
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Application publication date: 20190111 |