CN101898915A - Microbial agent suitable for crops in seedling stage and preparation method thereof - Google Patents

Microbial agent suitable for crops in seedling stage and preparation method thereof Download PDF

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Publication number
CN101898915A
CN101898915A CN 200910084799 CN200910084799A CN101898915A CN 101898915 A CN101898915 A CN 101898915A CN 200910084799 CN200910084799 CN 200910084799 CN 200910084799 A CN200910084799 A CN 200910084799A CN 101898915 A CN101898915 A CN 101898915A
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microbiobacterial agent
bacteria
seedling stage
solid fungicide
composite bacteria
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CN101898915B (en
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黄谦
于海燕
游环宇
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BEIJING JIABOWEN BIOSCIENCE Co Ltd
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BEIJING JIABOWEN BIOSCIENCE Co Ltd
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Abstract

The invention discloses a microbial agent suitable for crops in the seedling stage. The microbial agent is prepared by taking actinomycetes, bacillus, nitrogen-fixing bacteria, phosphorus bacteria, potassium bacteria, lactic acid bacteria, saccharomycetes, other beneficial microorganisms and metabolites thereof as the main components and adding a certain proportion of trace elements favorable for the growth of crops in the seedling stage. Owing to solid fermentation in the preparation process of the microbial agent, the product contains a large number of beneficial microorganisms, furthermore, the metabolites of the microorganisms are rich in various plant growth regulators, such as growth hormone, cytokinin, gibberellins and the like, thus achieving the obvious effects of breaking the seed dormancy, promoting the seed germination, increasing the emergence rate and promoting the root development.

Description

Be suitable for microbiobacterial agent of crops seedling stage use and preparation method thereof
Technical field
The present invention relates to a kind of microbial fertilizer and preparation method thereof, particularly relate to a kind of microbiobacterial agent that is suitable for the crops seedling stage use and preparation method thereof.
Background technology
The applicant is devoted to the research and development utilization of compound microbial culture starter for a long time, it is 200610083429.7 that the applicant has submitted application number on May 31st, 2006, name is called the Chinese patent application of " adopting composite bacteria that dining-kitchen organic waste is carried out the method that resources circulation is handled ", this patent of invention is now obtained the authorization, a kind of composite bacteria is wherein disclosed, it contains following bacterial classification: Bacillus circulans, Bacillus sphaericus, bacillus firmus, the Amur genus bacillus, bacstearothermophilus, subtilis, yeast, milk-acid bacteria, each bacterial classification can be individually according to a conventional method from the bacterial classification of cryopreservation the streak inoculation of picking bacterial classification to the solid plate substratum, cultivate down in conditions suitable, bacterium colony to be grown, be inoculated in liquid nutrient medium and carry out shaking culture, carry out enlarged culturing as required again; After each bacterial classification of above-mentioned cultivation can liquid form be cultivated, ratio in the viable count of Bacillus circulans, Bacillus sphaericus, bacillus firmus, Amur genus bacillus, bacstearothermophilus, subtilis, yeast, milk-acid bacteria: (0.8-1.1): (1.2-1.5): (1-1.2): (1.4-1.6): (2.2-3): (1.2-1.5): (1-1.2): (0.8-1.2), be mixed and made into liquid fermentation agent, standby in 4 ℃ of preservations; Also can preserve instant mixing, the instant use separately; Also the liquid culture lyophilize can be obtained solid bacterium powder, make the fermenting agent of solid form.The applicant finds that this composite bacteria can produce such as plurality of enzymes such as proteolytic enzyme, lipase, amylase, chitinases, can be micromolecular polypeptide and oligosaccharides with high molecular weight protein in the organism and fat acid decomposition, aforementioned patent only is used in it on fermentative processing of dining-kitchen organic waste, the continuous ground further investigation of applicant finds that this composite bacteria its metabolic activity and meta-bolites in metabolic process also have good promoter action to the growth of plant crop, continue to have proposed the application on this basis.
Summary of the invention
The application quoted the patent No. be 200610083429.7 (publication number CN1850965A) Chinese invention patent full text as a reference.
The purpose of this invention is to provide and a kind ofly be suitable for crop by being seeded into the microbiobacterial agent that seedling stage, process was used, it can break seed dormancy, promotes seed germination, promotes root system development, improves the rice shoot resistance, improves the nursery soil environment, for rice shoot provides adequate nutrients.
A kind of microbiobacterial agent that is suitable for the crops seedling stage use, its following component by weight percent is formed:
Composite bacteria solid fungicide: 50%~70%;
The peat composed of rotten mosses: 5%~15%;
Streptomyces microflavus solid fungicide: 10%~20%;
Medical stone: 8%~12%;
Acceptable micro-inorganic salt: 1%~3%;
Described composite bacteria is that the patent No. is the disclosed composite bacteria that is suitable for dining-kitchen organic waste is carried out fermentative processing in 200610083429.7 the Chinese patent, and described composite bacteria solid fungicide and streptomyces microflavus solid fungicide are the products of spawn culture postlyophilization;
Living bacteria count in the described microbiobacterial agent 〉=200,000,000/gram.
Microbiobacterial agent of the present invention, it preferably is made up of the following component of weight percent:
Composite bacteria solid fungicide: 60%;
The peat composed of rotten mosses: 13%;
Streptomyces microflavus solid fungicide: 15%;
Medical stone: 10%;
Acceptable micro-inorganic salt: 2%.
Microbiobacterial agent of the present invention, its water content are less than or equal to 12.0 weight %, and organic carbon content is more than or equal to 47 weight %.
Microbiobacterial agent of the present invention, wherein the peat composed of rotten mosses is being commonly called as of meadow peat, is before 5,000 and even 10,000 years on the bottom land, plant is withered year after year, is half state that rots, and piles up the organic ore body that forms year by year.The peat composed of rotten mosses has water-retentivity, and ventilation property can be as good microbe carrier, and has the effect of improving the soil.The peat composed of rotten mosses contains abundant humic acids, is the organism of pure natural, and is nontoxic aseptic.The peat composed of rotten mosses also contains abundant nitrogen, phosphorus, potassium etc.Use the peat composed of rotten mosses to substitute the part complex bacteria culture, can reduce the part producing cost.Medical stone mainly is used as carrier and some trace elements is provided, and possesses about soil improvement simultaneously.The production of this microbiobacterial agent also needs additionally to add the required trace element of plant-growth, as zinc, iron, selenium, copper and boron etc.It can add in the microbial inoculum by the acceptable inorganic salt form of plant, as zinc sulfate 0.5%, ferric sulfate 0.5%, inclined to one side selenic acid 0.4%, copper sulfate 0.3% and borax 0.3% etc.
The preparation method of microbiobacterial agent of the present invention mixes the final vacuum packing in proportion after described component pulverized.Finished state is a powdery, and fineness reaches 80 orders, and water content is less than or equal to 12.0 weight %, living bacteria count 〉=200,000,000/gram wherein, and organic carbon content is more than or equal to 47 weight %.
Microbiobacterial agent of the present invention, can be with reference to following using method during use:
(1) seedbed is spread fertilizer over the fields or directly dressed seed, soaks seed: 500g evenly spreads fertilizer over the fields in 15m with this product 2The seedbed.The Different Crop kind is used by existing method when seed dressing, seed soaking.
(2) cave dish and feeding block seedlings raising: mix nutrition soil or matrix 100~120kg with this product 500g, evenly adorn seeding and seedling raising behind the alms bowl.
(3) water seedling stage and execute: at crops seedling stage, convert clear water 45kg with this product 500g at every turn and evenly spray or convert clear water 100kg and water and impose on the seedbed, logotype in 7 days is 2~3 times at interval.
(4) before transplanting seedling stage, use: dip in root convert clear water 5kg transplanting with this product 500g before, perhaps convert clear water 100kg and water and impose on the seedbed with this product 500g.
The microbiobacterial agent that is suitable for the crops seedling stage use of the present invention has following beneficial effect:
(1) breaks seed dormancy, promote seed germination.Use this microbiobacterial agent during seed dressing, therein in the beneficial microorganism vital movement process, produce some meta-bolitess and induce the inner germination material of seed, as some hormones such as indolylacetic acid and its derivative and Plant hormones regulators,gibberellins, strengthen the respiratory metabolism of seed, strengthen the activity of enzyme, make the nutritive substance running of storage in the seed, embryo portion cell begins division, elongation, and radicle and plumular axis begin growth, prominent breaking in the seed coat.
(2) promote root growth and development, fibrous root prosperity.This microbiobacterial agent is by the physicochemical character of improving the soil to the effect of seedlings root growth on the one hand, for good soil nutrient and microbial environment have been created in the growth of plant root; Be that the beneficial microorganism meta-bolites has the plant hormone function on the other hand, stimulate crop root to grow to realize.
(3) make that the crop seedling is strong, blade is loose dark green, promote plant growth.Secrete organic acid with the beneficial microorganism after nursery soil cooperates, the nutrient that quickens in the medium of seedling bed is converted into the form that crop directly absorbs, and replenishes the required various elements of crop rapidly, promotes plant growth.
(4) improve seedling quality, strengthen crop anti-adversity.In seed dressing period, this microbiobacterial agent surrounds seed-coat, forms dominant microflora, with pathogenic bacteria contention available stock, grows thereby suppress growth of pathogenic bacteria, eradicates issuable disease of later stage in the early stage.
(5) seedling-slowing stage is short, and it is vigorous to grow.Before the seedling field planting, be transplanted into the land for growing field crops after can using this microbiobacterial agent to dip in root, microbial bacteria is known from experience ramp, induces plant Fa Xingen early, has shortened transplanting seedling time.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1: a kind of microbiobacterial agent that is suitable for the crops seedling stage use, and its following component by weight percent is formed:
Composite bacteria solid fungicide: 60%;
The peat composed of rotten mosses: 13%;
Streptomyces microflavus solid fungicide: 15%;
Medical stone: 10%;
Acceptable micro-inorganic salt: 2%, wherein zinc sulfate 0.5%, ferric sulfate 0.5%, selenic acid 0.4%, copper sulfate 0.3% and borax 0.3% partially.
Mix the final vacuum packing after said components pulverized in proportion.Finished state is a powdery, and fineness reaches 80 orders, and water content is less than or equal to 12.0 weight %, living bacteria count 〉=200,000,000/gram wherein, and organic carbon content is more than or equal to 47 weight %.
Embodiment 2: except that the amounts of components of forming microbiobacterial agent was distinguished to some extent, all the other were all identical with embodiment 1, and described component and consumption are:
Composite bacteria solid fungicide: 50%;
The peat composed of rotten mosses: 15%;
Streptomyces microflavus solid fungicide: 20%;
Medical stone: 12%;
Acceptable micro-inorganic salt: 3%, wherein zinc chloride 0.6%, iron protochloride 0.6%, selenic acid 0.6%, cupric chloride 0.6% and borax 0.6% partially.
Embodiment 3: except that the amounts of components of forming microbiobacterial agent was distinguished to some extent, all the other were all identical with embodiment 1, and described component and consumption are:
Composite bacteria solid fungicide: 70%;
The peat composed of rotten mosses: 5%;
Streptomyces microflavus solid fungicide: 16%;
Medical stone: 8%;
Acceptable micro-inorganic salt: 1%, wherein zinc phosphate 0.2%, ferrous phosphate 0.2%, selenic acid 0.2%, copper sulfate 0.2% and borax 0.2% partially.
Embodiment 4: except that the amounts of components of forming microbiobacterial agent was distinguished to some extent, all the other were all identical with embodiment 1, and described component and consumption are:
Composite bacteria solid fungicide: 65%;
The peat composed of rotten mosses: 13%;
Streptomyces microflavus solid fungicide: 10%;
Medical stone: 10%;
Acceptable micro-inorganic salt: 2%, wherein zinc sulfate 0.5%, ferric sulfate 0.5%, selenic acid 0.4%, copper sulfate 0.3% and borax 0.3% partially.
Test example: manure trial
Green house of vegetables has carried out the fertilizer test of microbiobacterial agent on shallot of embodiment 1~4 in Beijing Da Dong stream vegetables, strawberry cultivating base.Experimental field soil is light loamy texture cinnamon soil, 10 mu of test area, and test is " long pleasing " with green onion breed.
For trying fertilizer: present embodiment microbiobacterial agent, uniconazole, root-inducing powder and clear water.
Test method:
(1) uniconazole: seed with 10mg/L times of liquid seed soaking of uniconazole 12 hours, is mixed the peat composed of rotten mosses (500g/10m 2) broadcast in the soil.
(2) root-inducing powder: seed with 30mg/L times of liquid seed soaking of root-inducing powder 12 hours, is mixed the peat composed of rotten mosses (500g/10m 2) broadcast in the soil.
(3) microbiobacterial agent: seed was soaked seed 12 hours mixing microorganisms microbial inoculum (500g/10m with pure water 2) broadcast in the soil.
(4) clear water: seed with pure water seed soaking 12 hours, is mixed the peat composed of rotten mosses (500g/10m 2) broadcast in the soil.
Seedling stage, (after planting 20 days) were measured shallot overground part stem height.
Test-results sees the following form:
Test-results shows that the stem length that microbiobacterial agent is handled than clear water, uniconazole and root-inducing powder has raising respectively, and the microbiobacterial agent of embodiment 1 improves at most, respectively the long raising of handling than clear water, uniconazole and root-inducing powder 27.2%, 13.2%, 2.9% of stem.
As can be seen from the above embodiments, the embodiment of the invention is main component by adopting multiple beneficial microorganism such as actinomycetes, genus bacillus, vinelandii, phosphorus bacteria, potassium bacterium, milk-acid bacteria, yeast and meta-bolites thereof, and added a certain proportion of trace element of suitable crops seedling stage growth, make microbiobacterial agent, pass through solid fermentation in the use, make not only to contain a large amount of beneficial microorganisms in the product, and be rich in the various plants growth regulatory substance.Its activity substance content is 10~100 times of general bacterial fertilizer.Various plants growth substances such as the growth hormone that microbial inoculum produces during the fermentation, phytokinin, Plant hormones regulators,gibberellins, as the adjusting control agent of crop growth (especially seed, rice shoot), to breaking seed dormancy, promote seed germination, improve seedling rate, promoting that root system development plays a significant role.Microbial inoculum has improved the wherein quantity of beneficial functions bacterium after being manured into soil, promoted conversion and the release of solubility N, P, K in the organic substance, and the enrichment of promotes growth metabolic active substance.
The selected microorganism strains of this product all derives from soil or commodity purchasing.The microbiobacterial agent finished product that the present invention is suitable for the crops seedling stage use has following characteristic:
(1) soil bacteria: being made up of tens kinds of bacterial classifications such as dominant bacteria sporeformer, actinomycetes, milk-acid bacteria, yeast, is the composite microbial bacteria that derives from soil, the one-level safety standards.
(2) high-temperature aerobic bacterium: low to environmental requirement, homogeneity of product is strong, but commercial application.
(3) fermentation finished thoroughly1: in the fermentation process, animal protein and vegetable protein all are converted into tropina, form reconstituted product.Simultaneously, effectively kill various harmful pathogenic bacterias and virus;
(4) high-energy: microbiobacterial agent had both had the viable bacteria function from taking high energy, had trophic function again, the economic benefits and social benefits combination.
(5) high-level efficiency: beneficial microorganism becomes dormant state with the form of gemma under standard state, stable existence, and the back propagation at once that is manured into soil, with the soil bacteria synergy, product application efficient is very high.
(6) stability is strong: the beneficial microorganism rate of decay is slow, and effect is stable.Bacterial classification is without the indoor cultivation of going down to posterity, and vitality is strong.
The above only is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (9)

1. one kind is suitable for the microbiobacterial agent that crops seedling stage uses, and it is characterized in that its following component by weight percent is formed:
Composite bacteria solid fungicide: 50%~70%;
The peat composed of rotten mosses: 5%~15%;
Streptomyces microflavus solid fungicide: 10%~20%;
Medical stone: 8%~12%;
Acceptable micro-inorganic salt: 1%~3%;
Described composite bacteria is that the patent No. is the disclosed composite bacteria that is suitable for dining-kitchen organic waste is carried out fermentative processing in 200610083429.7 the Chinese patent, and described composite bacteria solid fungicide and streptomyces microflavus solid fungicide are the products of spawn culture after drying;
Living bacteria count in the described microbiobacterial agent 〉=200,000,000/gram.
2. microbiobacterial agent as claimed in claim 1 is characterized in that, its following component by weight percent is formed:
Composite bacteria solid fungicide: 60%;
The peat composed of rotten mosses: 13%;
Streptomyces microflavus solid fungicide: 15%;
Medical stone: 10%;
Acceptable micro-inorganic salt: 2%.
3. microbiobacterial agent as claimed in claim 1 or 2 is characterized in that, described microbiobacterial agent water content is less than or equal to 12.0 weight %, and organic carbon content is more than or equal to 47 weight %.
4. microbiobacterial agent as claimed in claim 1 or 2 is characterized in that, described trace element is zinc, iron, selenium, copper and boron.
5. microbiobacterial agent as claimed in claim 4 is characterized in that, described micro-inorganic salt are zinc sulfate 0.5%, ferric sulfate 0.5%, inclined to one side selenic acid 0.4%, copper sulfate 0.3% and borax 0.3%.
6. the preparation method of the described microbiobacterial agent of claim 1 is characterized in that, mixes the final vacuum packing after described component is pulverized in proportion.
7. the application of the described microbiobacterial agent of claim 1 when crops seedling stage.
8. application as claimed in claim 7, described microbiobacterial agent are spread fertilizer over the fields or are watered and impose on seedbed or directly seed dressing, seed soaking.
9. application as claimed in claim 7, described microbiobacterial agent is applied to crop root before crops seedling stage is transplanted.
CN 200910084799 2009-05-26 2009-05-26 Microbial agent suitable for crops in seedling stage and preparation method thereof Active CN101898915B (en)

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CN107466528A (en) * 2017-09-21 2017-12-15 界首市靳莓果酒有限责任公司 A kind of processing method for promoting strawberry germination
CN107493730A (en) * 2017-09-26 2017-12-22 界首市从生种植专业合作社 A kind of processing method for improving Angelica Sinensis Seeds germination percentage
CN107637217A (en) * 2017-10-21 2018-01-30 界首市铭硕园种植专业合作社 A kind of processing method for accelerating salvia seeds germination
CN107710957A (en) * 2017-11-03 2018-02-23 五河县金塘水产养殖有限公司 A kind of processing method for promoting water chestnut germination
CN107711381A (en) * 2017-11-21 2018-02-23 五河县金塘水产养殖有限公司 A kind of fast seedling-cultivating method of water chestnut
CN111348967A (en) * 2018-12-20 2020-06-30 深圳市芭田生态工程股份有限公司 Biological organic composite seedling culture regulator and preparation method thereof
CN112209762A (en) * 2019-07-12 2021-01-12 山东金必来生物科技有限公司 Macroelement water-soluble fertilizer containing microbial metabolite
CN113854078A (en) * 2020-06-30 2021-12-31 上海市药材有限公司 Astragalus membranaceus film-covering sand-pressing seedling raising method
CN113796391A (en) * 2021-08-25 2021-12-17 北京东方力原生物科技有限公司 Biological dormancy breaking agent

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