CN104788250A - An efficient nitrogen-fixing bacillus strain and a preparing method of a microbial fertilizer containing the strain - Google Patents
An efficient nitrogen-fixing bacillus strain and a preparing method of a microbial fertilizer containing the strain Download PDFInfo
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Abstract
The invention relates to a novel nitrogen-fixing bacillus strain and a preparing method of a microbial fertilizer containing the strain. The novel efficient nitrogen-fixing bacillus strain is obtained by separation from soil and is identified as bacillus amyloliquefaciens [J-25(1)]. By utilization of the J-25(1) and a specific granular carrier, the microbial fertilizer with an efficient nitrogen-fixing function is prepared. To ensure balance supply of nitrogen, phosphorus and potassium for plants, phosphorus-dissolving bacillus and potassium-dissolving bacillus are added into fermentation of the J-25(1), and phosphorus- and potassium- containing ore is added into the carrier so as to prepare the organic microbial granular fertilizer having the efficient nitrogen-fixing function and providing phosphorus and potassium. The fertilizer can meet nutrition requirements of vegetables, fruit trees and crops. Fertilization of a chemical fertilizer is not needed in a growth process of plants, thus saving energy, and avoiding pollution to the environment and water sources during chemical fertilizer production and application. In addition, the microbial fertilizer reduces soil-borne diseases, increases agricultural output, improves quality of agricultural products, and is obvious in economic, social and ecological benefit.
Description
Technical field
The present invention relates to a kind of new bacillus azotobacter [J-25 (1)] and the preparation method containing this bacterial strain production microbial fertilizer.
Background technology
China is a large agricultural country, and fertilizer occupies very important status in China's grain-production.But due to long-term to the excessive of chemical fertilizer with unreasonablely to use, also the problem that can not be ignored served by band: 1, polluted source: a large amount of use chemical fertilizer and soil erosion, nitrogen and phosphorus thing can to enter in surface water body with rainwash or under ooze, transverse movement is carried out by soil, then enter in lower water or surface water body, make surface water eutrophication, or direct polluted underground water.2, soil change is hardened: owing to using chemical fertilizer in a large number, and ignore using of fertilizer, soil fertility occurs that some fails, organic deficient.Ventilation property reduces, aerobic microorganism activity decrease, and soil-ripening is slow, cause soil compaction, crop root growth dysplasia, affects plant growth.3, salinification increases the weight of: excessive use chemical fertilizer after, in soil, salt ion increases, pH value raise.The soil salinization is increased the weight of, and overslaugh root system normally absorbs water, and affects plant strain growth.4, trace element deficiency: the vegetables etc. of continuous cropping for many years, absorb the trace element such as zinc, boron, molybdenum, copper, manganese in soil, there is no again the micro-fertilizer of supplementation administration, cause Trace Elements in Soil day by day to reduce, therefore seriously lack trace element, affect growing of the crops such as vegetables.5, active layer shoals: booth and outdoor vegetable many implementations stubble stubble intersection intercropping and interplanting, artificial farming, active layer shoals.6, disease and pest accumulation: continuous cropping makes germ insect pest accumulate in soil to increase, and harm increases the weight of, and thus root system is injured rotten, and even complete stool is withered.Because disease and pest is serious, Pesticide use amount increases, cause Vegetable pollution.Reducing the consumption of cost, is that traditional agriculture transforms important goal to the ecological agriculture.
Microbial fertilizer is a kind of environmental friendly fertilizer, and be also one of important directions of following agroforestry Sustainable development, it can maintain and increase soil fertility, and is conducive to the breeding of microorganism sociales, keeps ecological balance, energy-saving and emission-reduction, and the advantage such as easy to use.Therefore, microbial fertilizer is more and more favored by the mankind.China's microbial fertilizer development is in recent years very fast, and kind constantly increases.Because of reasons such as bacterial classification, nitrogen fixing capacity, production technology and formulas, the microbial fertilizer quality product of existing market is uneven.In microbial fertilizer, vinelandii kind, nitrogen fixing capacity, living bacteria count and miscellaneous bacteria amount are all the important indicators of quality product.Wherein species and quantity are the key problems determining microbial fertilizer fertilizer efficiency.
Summary of the invention
The object of the present invention is to provide a kind of novel, high-efficiency nitrogen-fixing microorganism, and described nitrogen-fixing microorganism is adsorbed on organic carrier, form organic microbial fertilizer.Under the condition that nitrogen-fixing microorganism temperature and humidity in soil is suitable, can amount reproduction, the molecular nitrogen voluntarily in fixed air, for plant provides nitrogenous source.Because no longer using chemical nitrogen fertilizer in growing process, not only save the energy, decreased the three waste discharge in synthetic ammonia process, also avoid the pollution of excessive fertilization to ambient environment and water source.Meanwhile, organic microbial fertilizer also can promote plant growth, reduces soil-borne disease and occurs, improve quality of agricultural product and quality.
An object of the present invention, is to provide that a strain is novel, the genus bacillus of high-efficiency nitrogen-fixing [J-25 (1)].
Object two of the present invention, is to provide a kind of formula being applicable to this high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] growth medium.
Object three of the present invention, is to provide microbial fertilizer that a kind of genus bacillus [J-25 (1)] bacterial classification that is novel by this, high-efficiency nitrogen-fixing is prepared from and preparation method thereof.
An object of the present invention can reach by taking following technical scheme:
The high-efficiency nitrogen-fixing genus bacillus (acillus amyloliquefaciens) that one strain is new, concrete, this bacterial classification is named as " J-25 (1) ", this bacterial classification is planted continuously the tomato soil of more than 10 years from the green house of vegetables in villages and small towns, room, Daiyue District, Tai'an, Shandong Province and is separated, and through domestication, preserve for a long time, its proterties of transferring stablize.
This is novel, high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] passes through 16S DNA sequencing on July 25th, 2014 by Shanghai Sheng Gong biotechnology company limited, prove that this high-efficiency nitrogen-fixing genus bacillus is bacillus amyloliquefaciens, biology name Bacillus amyloliquefaciens, gained sequence is shown in sequence table, order-checking unit address: No. 698, Xiang Min road, Shanghai Songjiang district, Sangon Biotech (Shanghai) Co., Ltd..
This is novel, the biological characteristics of high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] is white, and translucent, smooth moistening bacterium colony, slightly swells, circular neat bacterium colony; Gramstaining thalline purple is gram-positive microorganism, shaft-like; Make a living gemma aerophil, available protein, multiple sugar and starch, decompose tryptophane and form indoles.Growth temperature 25-55 DEG C, optimum growth temp 37 DEG C, optimal pH 6.8-7.8.Under bad condition, thalline transforms and produces gemma, and gemma, to poor environment strong stress resistance, can tolerate 100 DEG C of high temperature the short period of time.
The present invention also claimed one is applicable to substratum that this bacillus amyloliquefaciens (high-efficiency nitrogen-fixing genus bacillus [J-25 (1)]) grows and preparation method thereof.Specific as follows:
The substratum used in the present invention has:
No. 1 solid medium: peptone 10g/L, yeast powder 5g/L, sodium-chlor 10g/L, agar 18-20g/L, PH is adjusted to 7.2.
No. 1 liquid nutrient medium: this substratum is not containing agar, and other compositions are identical with No. 1 substratum with content, and PH is adjusted to 7.2.
No. 2 solid mediums: extractum carnis 3g/L, peptone 5g/L, glucose 2.5g/L, NaCl5g/L, agar 18-20g/L, adjusts pH to 7.2 with NaOH.
No. 2 liquid nutrient mediums: extractum carnis 3g/L, peptone 5g/L, glucose 2.5g/L, NaCl5g/L, adjust pH to 7.2 with NaOH.
No. 3 solid mediums: extractum carnis 5g/L, peptone 10g/L, NaCl5g/L, agar 18-20g/L, adjusts pH to 7.2 with NaOH.
No. 3 liquid nutrient mediums: extractum carnis 5g/L, peptone 10g/L, NaCl5g/L, adjust pH to 7.2 with NaOH.
No. 4 solid mediums: starch 5g/L, sucrose 1g/L, yeast extract 0.2g/L, peptone 5g/L, extractum carnis 5g/L, ammonium sulfate 1g/L, potassium primary phosphate 0.5g/L, magnesium sulfate 0.2g/L, sodium-chlor 5g/L, agar 18-20g/L, adjust pH to 7.2 with NaOH.
No. 4 liquid nutrient mediums: starch 5g/L, sucrose 1g/L, yeast extract 0.2g/L, peptone 5g/L, extractum carnis 5g/L, ammonium sulfate 1g/L, potassium primary phosphate 0.5g/L, magnesium sulfate 0.2g/L, sodium-chlor 5g/L, adjust pH to 7.2 with NaOH.
All substratum are all before use through high pressure steam sterilization (121 DEG C, 30 minutes) above, cool for subsequent use.
The substratum that the present invention uses during traditional microbial fertilizer also can be adopted to produce and cultural method prepare this microbial fertilizer.The culture condition of this bacterium is consistent with common bacteria, but for preventing its functional deterioration, need cultivate under sugar-free height salt condition.
The substratum that traditional microbial strains is used in producing: starch 100g/L, soybean cake powder 100g/L, dipotassium hydrogen phosphate 2g/L, ammonium sulfate 1g/L, magnesium sulfate 0.5g/L, chlorination 0.01g/L, calcium carbonate 0.1g/L, yeast extract paste 0.1g/L, PH7.2.
The concrete production method of high-efficiency nitrogen-fixing micro-organisms bacillus fertilizer:
One, [J-25 (the 1)] culture presevation of high-efficiency nitrogen-fixing genus bacillus and activation culture
Bacterial classification is with the preservation on No. 1 solid medium of streak culture mode, and short term storage condition is 4 DEG C, activates 1 every month; Long term storage condition is-20 DEG C, activation in every 6 months 1 time.
Two, the cultivation of high-efficiency nitrogen-fixing genus bacillus [J-25 (1)]
1, method provided by the invention is adopted to carry out the cultivation of high-efficiency nitrogen-fixing genus bacillus:
1) after Storaged media gets bacterial classification, line access No. 1 solid slant culture base, 37 DEG C of incubators are cultivated 36 hours under leaving standstill dark condition;
2) use aseptic water washing inclined-plane, form bacteria suspension;
3) by No. 1 liquid nutrient medium of 1 volume bacteria suspension access about 20 volumes, it is 10 that 37 DEG C of aerobics are cultured to cell concentration
9individual/ml order of magnitude;
4) by step 3) bacteria suspension that obtains joins in No. 2 liquid nutrient mediums of about 5-10 times volume, and it is 10 that 37 DEG C of aerobics are cultured to cell concentration
9individual/ml order of magnitude;
5) by step 4) bacteria suspension that obtains joins in No. 3 liquid nutrient mediums of about 50-100 times volume, and it is 10 that 37 DEG C of aerobics are cultured to cell concentration
9individual/ml order of magnitude;
6) by step 5) bacteria suspension that obtains joins in No. 4 substratum of about 5-10 times volume, and cultivate aerobics to be cultured to cell concentration be 10 for 37 DEG C
9individual/ml order of magnitude.
2, traditional microbiological fermentation method for producing is adopted to carry out the cultivation of high-efficiency nitrogen-fixing genus bacillus [J-25 (1)]
Substratum: starch 100g/L, soybean cake powder 100g/L, dipotassium hydrogen phosphate 2g/L, ammonium sulfate 1g/L, magnesium sulfate 0.5g/L, chlorination 0.01g/L, calcium carbonate 0.1g/L, yeast extract paste 0.1g/L, PH7.2.
To access in the above-mentioned substratum of 1L after actication of culture, triangular flask shaking flask 120r/min, cultivate 24 hours for 37 DEG C, then be inoculated in 250L seed fermentation tank by 1% inoculum size, ventilation, cultivates 24 hours, then receives in 2500L fermentor tank by 10% inoculum size for 37 DEG C, cultivate 24 hours for 37 DEG C, produce after gemma until 80% thalline and stop fermentation.
Three, the production of high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer
1) preparation of Particulate microbial fertilizer carrier, described particulate vector is made up of humic acid, xylogen, plant ash, bone calcium powder, feldspar in powder, ground phosphate rock and clay, the ratio shared in particulate vector of above-mentioned raw materials is 3.5: 2: 2.5: 1: 0.3: 0.1: 2, by raw material mixing granulation and dry for standby;
2) high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer product preparation, can reach by taking following technical scheme: the ratio of producing 1000kg bacterial manure according to 100L fermented liquid, to determine that the centrifugal gained of volume fermented liquid concentrates thalline and is dissolved in suitable quantity of water, it spraying is adsorbed on carrier, m bacteria suspension: m carrier=1: 10, then the carrier granule being adsorbed with thalline is mixed with starch, m starch: m particle=1: 10.
The present invention has following substantive distinguishing features:
1, the present invention is separated and obtains the new high-efficiency nitrogen-fixing genus bacillus of a strain [J-25 (1)] from soil, and have studied its cultivation, conservation, etc. condition, identify that clear and definite this bacterium is the new bacillus amyloliquefaciens (acillus amyloliquefaciens) of a strain.
2, the present invention is owing to arranging specific material formula and carry out fermentation culture under specific process conditions, therefore, prepares microbial fertilizer good product quality, toxigenic capacity is low and culture efficiency is high.
3, use fermentation mode provided by the invention to carry out the microbial fertilizer of fermentative production at this bacterial classification [J-25 (1)], through effective fungal counting during warehouse-in, effective bacterium number meets enterprise and national standard.The microbial fertilizer produced stores 2 years, and the product of new production contrasts in the application, nitrogen fixation effect no significant difference, and storage validity period can reach more than 2 years.
4, applicant takes new high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] and specific particulate vector formula, make the organic microbial granulated fertilizer with high-efficiency nitrogen-fixing effect, impose on when crop-planting in soil, under humiture suitable condition, the nitrogenous source supply in vegetables, fruit tree and farm crop whole vegetative period can be met, and there is outstanding effects such as improveing soil, growth promoting effects, improve the yield and quality.
5, the present invention ensures the nitrogen of plant, phosphorus, potassium balance supply, can add in high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] fermentation and separate Bacillus phosphorus and potassium decomposing genus bacillus, form the multi-functional flora of mixing, phosphorous and potassium ore deposit soil is added in processing formula, make existing high-efficiency nitrogen-fixing effect, the organic microbial granulated fertilizer of phosphorus and potassium supply can be provided again.
Embodiment
Specific embodiment 1
1, adopt method provided by the invention to carry out high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] to cultivate:
(1) after Storaged media gets kind, line access No. 1 solid slant culture base, 37 DEG C of incubators are cultivated 36 hours under leaving standstill dark condition;
(2) use aseptic water washing inclined-plane, form bacteria suspension;
(3) by No. 1 liquid nutrient medium of 1 volume bacteria suspension access about 20 volumes, it is 10 that 37 DEG C of aerobics are cultured to cell concentration
9individual/ml order of magnitude;
(4) by step 3) bacteria suspension that obtains joins in No. 2 liquid nutrient mediums of about 5-10 times volume, and it is 10 that 37 DEG C of aerobics are cultured to cell concentration
9individual/ml order of magnitude;
(5) by step 4) bacteria suspension that obtains joins in No. 3 liquid nutrient mediums of about 50-10 times volume, and it is 10 that 37 DEG C of aerobics are cultured to cell concentration
9individual/ml order of magnitude;
(6) by step 5) bacteria suspension that obtains joins in No. 4 substratum of about 5-10 times volume, and cultivate aerobics to be cultured to cell concentration be 10 for 37 DEG C
9individual/ml order of magnitude.
2, adopt traditional microbiological fermentation method for producing to carry out high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] to cultivate
Substratum: starch 100g/L, soybean cake powder 100g/L, dipotassium hydrogen phosphate 2g/L, ammonium sulfate 1g/L, magnesium sulfate 0.5g/L, chlorination 0.01g/L, calcium carbonate 0.1g/L, yeast extract paste 0.1g/L, PH7.2.
To access in the above-mentioned substratum of 1L after actication of culture, triangular flask shaking flask 120r/min, cultivate 24 hours for 37 DEG C, then be inoculated in 250L seed fermentation tank by 1% inoculum size, ventilation, cultivates 24 hours, then receives in 2500L fermentor tank by 10% inoculum size for 37 DEG C, cultivate 24 hours for 37 DEG C, produce after gemma until 80% thalline and stop fermentation.
3, the production of high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer
(1) preparation of Particulate microbial fertilizer carrier, described particulate vector is made up of humic acid, xylogen, plant ash, bone calcium powder, feldspar in powder, ground phosphate rock and clay, the ratio of above-mentioned raw materials shared by particulate vector is 3.5: 2: 2.5: 1: 0.3: 0.1: 2, by raw material mixing through rolling granulator granulation, dry for standby;
(2) microbial fertilizer product preparation, can reach by taking following technical scheme: the ratio of producing 1000kg bacterial manure according to 100L fermented liquid, the thalline of volume efficient bacillus azotobacter [J-25 (1)] the centrifugal gained of fermented liquid will be determined, be dissolved in suitable quantity of water, in mixing machine, carrier is sprayed, m bacteria suspension: m carrier=1: 10, the carrier granule that then will be adsorbed with high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] thalline mixes with starch, m starch: m particle=1: 10.
Embodiment 1: carry out fungal counting to the microbial fertilizer utilizing method provided by the invention to prepare under the condition of not long-time stock, dilution plate number scale conveniently detects the viable bacteria content in microbial strains agent.Take microbial strains agent 1 gram (stock's two weeks), with sterilized water dissolve, on shaking table shake 20 minutes, obtain bacteria suspension, get this bacteria suspension 0.5mL, add in 4.5mL aqua sterilisa, mix 5 × 10
-1bacteria suspension, and by that analogy, continuing to obtain concentration is 5 × 10
-2, 5 × 10
-3, 5 × 10
-4, 5 × 10
-5with 5 × 10
-6bacteria suspension.Be 5 × 10 by concentration
-5with 5 × 10
-6bacteria suspension respectively get 30 μ L on flat board, smear evenly with spreader, each concentration repeats 4 times.Carry out enumeration after 37 DEG C of cultivation 24h, and finally convert as fermented liquid institute bacteria containing amount according to weaker concn.Enterprise's control criterion:>=2.0*10
8individual/gram.
Embodiment 2: carry out fungal counting to the microbial fertilizer utilizing method provided by the invention to prepare under the condition of not long-time stock, dilution plate number scale conveniently detects the viable bacteria content in microbial fertilizer.Take microbial fertilizer 1 gram (stock 1 year), with sterilized water dissolve, on shaking table shake 20 minutes, obtain bacteria suspension, get this bacteria suspension 0.5mL, add in 4.5mL aqua sterilisa, mix 5 × 10
-1bacteria suspension, and by that analogy, continuing to obtain concentration is 5 × 10
-2, 5 × 10
-3, 5 × 10
-4, 5 × 10
-5with 5 × 10
-6bacteria suspension.Be 5 × 10 by concentration
-5with 5 × 10
-6bacteria suspension respectively get 30 μ L on flat board, smear evenly with spreader, each concentration repeats 4 times.Carry out enumeration after 37 DEG C of cultivation 24h, and finally convert as fermented liquid institute bacteria containing amount according to weaker concn.Enterprise's control criterion:>=2.0*10
8individual/gram.
Embodiment 3: the effect of this novel microorganism fertilizer is as follows:
Tested on tomato, cucumber and summer squash by the microbial fertilizer made with high-efficiency nitrogen-fixing genus bacillus [J-25 (1)], 3 repetitions are established in test.During test, microbial fertilizer product is spreaded manuer in holes by 600-800 kg/acre.Conventional vegetable fertilizer 1000 kgs/acre is spreaded manuer in holes.Tomato composite fertilizer special every mu executes 500-600 kilogram, and wherein 300 kilograms all as base manure; Fruit expanding period and initial stage of gathering respectively impose 1 time, each 100,150 kilograms.Tomato in plastic greenhouse field test results is in table 1 (in table, data are the volume increase per-cent of each fertilizer relative to blank).
The impact (Taian Shandong room villages and small towns Xi Liangfu village 2013) of different fertilizer on tomato yield used by table 1
In table, data carry out data analysis through statistic software SPSS, the significant difference under p=0.05 level of the different letter representations in same row, lower same.
During plastic tent cucumber test, microbial fertilizer product is spreaded manuer in holes by 600-800 kg/acre.Conventional vegetable fertilizer 1000 kgs/acre is spreaded manuer in holes.Special compound fertilizer for cucumber every mu executes 500-600 kilogram, and wherein 300 kilograms all as base manure; Fruit expanding period and initial stage of gathering respectively impose 1 time, each 100,150 kilograms.Plastic tent cucumber field test results is in table 2.
The impact (Taian Shandong room villages and small towns Bei Teng village 2013) of different fertilizer on cucumber yield used by table 2
During the test of booth summer squash, microbial fertilizer product is spreaded manuer in holes by 400-600 kg/acre.Conventional vegetable fertilizer 800 kgs/acre is spreaded manuer in holes.Summer squash composite fertilizer special every mu executes 300-400 kilogram, and wherein 200 kilograms all as base manure; Fruit expanding period and initial stage of gathering respectively impose 1 time, each 50,100 kilograms.Booth summer squash field test results is in table 3.
The impact (Taian Shandong room villages and small towns Xi Liangfu village 2013) of different fertilizer on summer squash output used by table 3
Field test results shows, this microbial fertilizer is used when booth vegetable is transplanted, spread manuer in holes, all can ensure the Nitrogen supplying of the vegetables such as tomato, cucumber and summer squash, comparatively use the process obvious effect of increasing production of conventional fertilizer and composite fertilizer for every mu 600-800 kg/acre.
Claims (8)
1., containing a preparation method for new high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer, it is characterized in that the particulate vector that high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] new containing a strain in this microbial fertilizer and particular mine material component are made.
2. according to claim 1 a kind of containing new high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer, it is characterized in that the new high-efficiency nitrogen-fixing genus bacillus of a contained strain is for [bacillus amyloliquefaciens, acillus amyloliquefaciens, J-25 (1)], the biological characteristics of this bacterial classification is white, translucent, smooth moistening bacterium colony, slightly swell, circular neat bacterium colony; Gramstaining thalline purple is gram-positive microorganism, shaft-like; Make a living gemma aerophil, available protein, multiple sugar and starch, decompose tryptophane and form indoles.Growth temperature 25-55 DEG C, optimum growth temp 37 DEG C, optimal pH 6.8-7.8.Under bad condition, thalline transforms and produces gemma, and gemma, to poor environment strong stress resistance, can tolerate 100 DEG C of high temperature the short period of time.
3. the culture condition of this bacterium is consistent with common bacteria, but for preventing its functional deterioration, need cultivate under sugar-free height salt condition.
4. according to claim 1 a kind of containing new high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer, it is characterized in that described particulate vector is formed through mixing granulation by humic acid, xylogen, plant ash, bone calcium powder, feldspar in powder, ground phosphate rock and clay.
5. according to claim 1 a kind of containing new high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer, it is characterized in that the ratio shared in particulate vector of humic acid in described particulate vector, xylogen, plant ash, bone calcium powder, feldspar in powder, ground phosphate rock and clay is 3.5: 2: 2.5: 1: 0.3: 0.1: 2, by raw material mixing granulation and dry for standby.
6. according to claim 1 a kind of containing new high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer, it is characterized in that the ratio of producing 1000kg bacterial manure according to 100L fermented liquid, to determine that the centrifugal gained thalline of volume fermented liquid is dissolved in suitable quantity of water, spray to carrier, oven drying at low temperature obtains microbial fertilizer.
7. according to claim 1 a kind of containing new high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer, it is characterized in that the nitrogen for ensureing plant, phosphorus, potassium balance supply, can add in high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] fermentation and separate Bacillus phosphorus and potassium decomposing genus bacillus, form the multi-functional flora of mixing.
8. according to claim 1 a kind of containing new high-efficiency nitrogen-fixing genus bacillus [J-25 (1)] microbial fertilizer, it is characterized in that in particulate vector processing formula, to add phosphorous and potassium ore deposit soil, make existing high-efficiency nitrogen-fixing effect, the organic microbial granulated fertilizer of phosphorus and potassium supply can be provided again.
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| CN111235054A (en) * | 2020-01-14 | 2020-06-05 | 南京大学 | Saline soil improvement microbial inoculum and preparation method thereof |
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| CN110372438A (en) * | 2019-06-26 | 2019-10-25 | 山东慧泰华生物技术有限公司 | A kind of fertilizer special for vegetable material and its preparation method and application |
| CN111235054A (en) * | 2020-01-14 | 2020-06-05 | 南京大学 | Saline soil improvement microbial inoculum and preparation method thereof |
| CN112464560A (en) * | 2020-11-25 | 2021-03-09 | 吉林农业大学 | Circular agriculture multi-target planting and breeding scale distribution optimization method based on particle swarm optimization |
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