CN103725635A - Compound probiotics for strawberry planting - Google Patents

Compound probiotics for strawberry planting Download PDF

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Publication number
CN103725635A
CN103725635A CN201310747377.9A CN201310747377A CN103725635A CN 103725635 A CN103725635 A CN 103725635A CN 201310747377 A CN201310747377 A CN 201310747377A CN 103725635 A CN103725635 A CN 103725635A
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fermentation
yeast
acid bacteria
bacterium
probiotic
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陈爱家
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SHAANXI WANYUAN BIOLOGICAL AGRICULTURAL TECHNOLOGY Co Ltd
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SHAANXI WANYUAN BIOLOGICAL AGRICULTURAL TECHNOLOGY Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention relates to compound probiotics for strawberry planting. The probiotics can be applied through soil treatment, manufacturing of bacterial manure, irrigation, foliage spray, ground spray and air atomization and bacteria removal. The yield, quality and disease resistance of strawberries are improved.

Description

A kind of compound probiotic for strawberry cultivating
Technical field
The invention belongs to microbial technology field, more specifically, relate to a kind of compound probiotic for strawberry cultivating.
Background technology
Strawberry property Xi Shui, happiness sunshine, be applicable to planting good in hydrophobicity, ventilation property is good is rich in organic sand, during defective aeration, root growth is obstructed.Strawberry happiness neutrality or subacidity soil, the soil plantation that more suitable pH value is 5.5 ~ 7.0.
Existing strawberry cultivating mostly is greenhouse gardening, mostly there is continuous cropping situation in strawberry cultivating soil, too much use chemical fertilizer and the farm manure not becoming thoroughly decomposed in addition, and its soil physico-chemical property often changes, some beneficial microorganism growths are suppressed, and some harmful microorganisms are bred rapidly; The Balance of nature of soil microorganisms is destroyed, so not only cause the obstacle of fertilizer decomposition course, and disease and pest occur many, spread soon, and increase the weight of year by year, the particularly root disease such as some common leaf molds, gray mold, oidium, blight, root rot, causes strawberry Severe Reduction, quality to decline.There is these situations, can, by using compound probiotic, to strawberry, carry out rationally effectively Cultivate administration.
By using composite probiotics ferment farm manure, coordinate again the compound probiotic of finite concentration proportioning to irrigate, soil can be maintained to the subacidity that strawberry adapts to, and utilize the water-retentivity of microorganism to keep ground moistening, to ensure the primary condition of its growth.
Summary of the invention
One. the effect of compound probiotic in plantation
1. the part of plant on earth's surface is cauline leaf, and it is carrying out photosynthesis, carrying out energy transformation.
A. using the compound probiotic that contains milk-acid bacteria as foliage fertilizer, be sprayed on plant leaf and limb, milk-acid bacteria can increase the effect of light compositing bacterium, and light compositing bacterium can promote the photosynthesis of plant, the formation of fortification of plants body to vitamin C and E, therefore, by being sprayed at plant leaf and limb above take milk-acid bacteria as main compound probiotic, can promote the photosynthesis of plant, produce a large amount of chlorophyll, guaranteed luxuriance of vegetation and health.
B. as pathogenic bacteria and viral natural enemy, the safest sterilizing of milk-acid bacteria or diseases and pests of agronomic crop and sterilant.After the compound probiotic that contains milk-acid bacteria is diluted according to a certain percentage, be sprayed at plant leaf and limb upper, sprayed the cauline leaf surface journey subacidity after milk-acid bacteria, order about insect escape, also the edible food of insect that just broken, insect cannot, in the existence of cauline leaf environment, reach the effect of desinsection expelling parasite; Thereby realize, kill some pathogenic microorganisms and kill some insect, as agrotis larva (formal name used at school: prodenia litura), native acarid (tick), various leaf tick worm, larva take great Ye grass as food etc.Avoid a large amount of use chemical insecticides, guaranteed food safety.
2. the part of plant under earth's surface is mainly root system, and it makes plant from soil, draw various desired nutritionals, carrying out matter and energy conversion.
A. by by spoilage organism etc. cause corrupt soil, germicidal treatment before planting; A certain proportion of compound probiotic that contains milk-acid bacteria is sprayed, turned over, thereby suppress the growth of spoilage organism, make it without living space, prevent root rot.This is milk-acid bacteria " soil disinfectant " function.
B. utilize take milk-acid bacteria as main compound probiotic and carry out compost treatment, make microbial organic fertilizer, the soil before plantation is carried out to microbial organic fertilizer and topdress, can improve crop yield by fertilising, improve product quality, maintain and increase soil fertility.Increase soil nutrient, avoid the too much chemical fertilizer of using.
C. the compound probiotic that contains milk-acid bacteria by a certain percentage adds water and irrigates, and improves soil microorganisms environment and root exudates environment, makes its equilibrium, makes plant root more easily absorb nutrient; A point stem phase to plant plays a part very important.
D. because milk-acid bacteria is sugary and lactic acid, and earthworm has a sharp sense of taste, happiness sweet food and tart flavour, therefore can amount reproduction as the earthworm in soil, and earthworm can make loosing soil, make moisture and fertilizer be easy to enter and improve the fertility of soil, be conducive to the growth of plant.
E. soil heat preservation function: due to milk-acid bacteria anaerobism breeding under soil table, driven a series of beneficial microorganism activities, these microorganism growth metabolism are that the soil moisture improves 1-2 ℃.In the north, can play the function of protecting plant root.
F. soil water-retaining function: because milk-acid bacteria is microbial bacterial, among its cells in vivo, moisture is approximately 70%, while having a large amount of milk-acid bacteria, can keep soil moisture.Aerobe is survived at upper soll layer, and anaerobies bacterium survives under earth's surface, and spoilage organism cannot be survived under earth's surface, guarantees that plant seed issues blastogenesis root, growth at the environment of safety, forms gradually ground flora, and then reaches water and soil and do not run off.
G. trophism: take milk-acid bacteria as main compound probiotic is in growth metabolism process, produce multivitamin, organonitrogen, soluble phosphate, amino acid etc., when plant root nutrition can be provided, can also obviously increase the content of food protein, sugar, VITAMIN and aromatoising substance, increase the dry-matter proportion of fresh and tender food, thereby Food Quality, local flavor, keeping quality are improved, color and luster, outward appearance etc. are obviously improved, and this special role is that chemical fertilizer is irreplaceable.
H. after the compound probiotic that contains milk-acid bacteria being combined with other fibrous substances, join in soil, dig under earth's surface, carry out spontaneous fermentation, can reduce gradually nitrate ion concentration, solve acidification problem.
Under the effect of the compound probiotic that contains milk-acid bacteria, after optimumization of plant rhizosphere environment, plant stem-leaf is also just grown up healthy and sound, and insect cannot be invaded food from root; To depending on the aquaculture of plant, be also very useful, plant is as the beginning of cultivation chain, to the prevention of aquaculture pathogenic bacteria and virus, do not need to re-use antibiotic etc, guaranteed the pure natural property of plant, meanwhile, also reduce the residual harm bringing to people's health of environmental pollution and agricultural chemicals, thus the world that can realize nature probiotic bacterium and human life's symbiosis.
Two, the compound probiotic of different purposes forms
1. " compound probiotic " that soil remediation is used: containing milk-acid bacteria, yeast, actinomycetes bacterium, photosynthetic bacterium, genus bacillus etc.
2. make " compound probiotic " that microbial-bacterial fertilizer is used: containing compound probiotics such as milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus.
3. " compound probiotic " that pouring is used: containing compound probiotics such as milk-acid bacteria, yeast, nitrobacteria, genus bacillus.
4. cauline leaf sprays use " compound probiotic ": the compound probiotics such as photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus.
5. ground sprays use " compound probiotic ": the compound probiotics such as milk-acid bacteria, yeast, genus bacillus.
6. " compound probiotic " that air atomizing degerming is used: the compound probiotics such as milk-acid bacteria, yeast, genus bacillus.
Three. microbial species planting technology is as follows at the concrete function of strawberry cultivating:
1. increase soil fertility, chessom, maintenance soil moisture, form good rhizospheric environment, promote, outside root absorption nitrogen phosphorus potassium, to absorb iron, zinc, magnesium, manganese etc. simultaneously, impel plant leaf more healthy carry out photosynthesis.
2. increase fruit resistance, can effectively kill spoilage organism in soil, prevent and treat root rot and disease and pest; As control of the common leaf mold of strawberry, gray mold, oidium, blight etc. etc.
3. promote photosynthesis, make the more flourishing of fruit growth.
4. improve fruit quality, prolong storage period.
5. increasing both production and income.
6. reduce continuous cropping continuous cropping obstacle.
Embodiment
The obtain manner of compound probiotic.
Milk-acid bacteria, yeast, subtilis, photosynthetic bacterium, actinomycetes, nitrobacteria bacterial classification are all purchased from market.
Produce the fermented liquid of milk-acid bacteria, yeast, subtilis, photosynthetic bacterium, actinomycetes, nitrobacteria, then above-mentioned fermented liquid is composited in proportion first respectively.
The production method of milk-acid bacteria is:
In 1kg water, add brown sugar 0.05~1.0kg, be configured to after solution, add 5g~10g lactic acid bacteria culturers, sealed fermenting in anaerobic fermentation tank, 33~38 ℃ of controlled fermentation temperature, fermentation time 3~7 days, the liquid spawn having activated is added to second order fermentation tank sealed fermenting, second order fermentation liquid proportioning is: molasses 10%, amino acid 0.1%~0.5%, 33~38 ℃ of VITAMIN 0.05% ~ 1% controlled fermentation temperature, fermentation time 3~7 days, inoculum size 5%~10%, between yeast phase, constantly add molasses, treat that bacterial classification concentration reaches 300,000,000/ml, pH is 4.2 o'clock, stop fermentation.
Saccharomycetic production method is:
By being inoculated into bacterial classification in yeast, expand in fermented liquid, the formula of fermented liquid is: glucose 50g/L, urea (NH 4) 2sO 41g/L, KH 2pO 42.5g/L Na 2hPO 40.5g/L, MgSO 41g/L, FeSO 40.1g/L, yeast extract paste 0.5g/L, rose-bengal 0.03g/L, pH4.5-5.0, sealed fermenting in aerobic fermentation tank, controlled fermentation temperature 25-28 ℃, fermentation time 2-3 days, the liquid spawn having activated is added to the fermentation of secondary aerobic fermentation tank, second order fermentation liquid proportioning is: soybean sprout 100g/L, glucose 50g/L, pH nature, controlled fermentation temperature 25-28 ℃, fermentation time 3~5 days, inoculum size 5%~10%, constantly adds glucose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation.
The production method of subtilis is: subtilis is seeded in substratum, culture medium prescription is: peptone 1 %, yeast extract 0. 5 %, sodium-chlor 1 %, nature pH, is placed in 37 ℃, seed fermentation tank and cultivates after 1-2 days, access fermention medium, culture medium prescription is: sucrose 1 %, peptone 1 %, Sodium phosphate dibasic 0. 2 %, SODIUM PHOSPHATE, MONOBASIC 0. 2 %, in natural pH, inoculum size 5%~10%, 35~37 ℃ of temperature, constantly add sucrose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation.
The production method that it is characterized in that described photosynthetic bacterium is: photosynthetic bacterium is seeded in yeast extract paste protein culture medium, and basic recipe is: CaCL 20.3g, MgSO 47H 2o 0.5g, yeast extract paste 3g, peptone 3g, distilled water 1000ml, adjusts pH 6.8, in culture temperature: 25 ℃~30 ℃, intensity of illumination: 2000LX~5000LX, lower cultivation 3 days, afterwards in aerobic fermentation tank with 15% inoculum size, 25 ℃~30 ℃ of temperature, intensity of illumination: 2000LX~5000LX, cultivates under the condition of pH:7.5~8.5, and in fermenting process, results of regular determination pH also carrys out neutralise broth with 150g/L Sulfothiorine, until bacterial classification concentration, reach 300,000,000/ml when above, stop fermentation.
Actinomycetic production method is: bacterial classification is inoculated in No. 1 substratum of Gao Shi (GA), and main component is: Zulkovsky starch 20g, KNO 31g, K 2hPO 40.5g, MgSO 47H 2o 0.5g, NaCl 0.5g, FeSO 47H 2o 0.01g, pH=7.4-7.6, water 1000ml enlarged culturing is controlled 28~30 ℃ of temperature in same medium with 5%~10% inoculum size afterwards in aerobic fermentation tank, and pH7.0-7.6 fermentation culture reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation.
The production method of nitrobacteria is: nitrobacteria is seeded in substratum, and culture medium prescription is: ammonium sulfate 0.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, SODIUM PHOSPHATE, MONOBASIC 1g, magnesium sulfate 0.03g, CaCl 27.5g, distilled water 1000ml, in pH 7.5~8.0, enlarged culturing 3 days under 28~30 ℃ of conditions of temperature, with 5%~10% inoculum size, in the aerobic large fermentation tank of same medium, control 28~30 ℃ of temperature afterwards, pH7.5-8.0 fermentation culture, reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation.
The composition of compound probiotic:
1. " compound probiotic " that soil remediation is used: milk-acid bacteria, yeast, genus bacillus, photosynthetic bacterium, actinomycetes by weight compositely proportional are: (5~8): (1~3): (0~1): (1~2): (0~1), preferably (5~6): (1~2): (0~1): (1~2): (0~1), most preferred ratio is 5:1:1:2:1.
2. make " compound probiotic " that microbial-bacterial fertilizer is used: milk-acid bacteria, yeast, genus bacillus, actinomycetes compositely proportional is by weight: (3~5): (1~2): (3~5): (1~2), preferably (3~4): (1~2): (4~5): (1~2), most preferred ratio is 3:1:4:1.
3. " compound probiotic " that pouring is used: milk-acid bacteria, yeast, genus bacillus, nitrobacteria by weight compositely proportional are: (5~8): (1~3): (1~2): 1, preferably (6~7): (1~2): (1~2): 1, most preferred ratio is: 6:2:1:1.
4. cauline leaf sprays use " compound probiotic ": milk-acid bacteria, yeast, photosynthetic bacterium, genus bacillus by weight compositely proportional are: (5~8): (1~3): (0~1): (1~2), preferably (5~6): (1~2): 1:(1~2), most preferred ratio is 5:2:1:2.
5. ground sprays use " compound probiotic ": milk-acid bacteria, yeast, genus bacillus by weight compositely proportional are: (5~8): (1~3): (1~2), what more have choosing is (6~8): (1~2): 1, and most preferred ratio is 8:1:1.
6. " compound probiotic " that air atomizing degerming is used: milk-acid bacteria, yeast, genus bacillus by weight compositely proportional are: (5~7): (2~3): (1~2), what more have choosing is (6~7): (2~3): 1, and most preferred ratio is: 6:3:1
The compound probiotic that contains milk-acid bacteria is taken as the leading factor with Lactobacterium acidophilum, the carbohydrate that it produces by picked-up photosynthetic bacteria, yeast carries out growth metabolism, produce lactic acid, milk-acid bacteria has very strong sterilizing ability, can effectively suppress the movable and organic sharply corrupt decomposition of harmful microbe.It can decompose not labile xylogen and Mierocrystalline cellulose under normality, and organic matter fermentation is decomposed.
According to the situation of the existing soil in strawberry cultivating garden, administer in accordance with the following steps:
If the disease that 1. exists spoilage organism to cause in soil, needs with the compound probiotic sterilizing that contains milk-acid bacteria and in conjunction with irrigation method, existing soil is carried out to sterilising treatment (implementation method refers to embodiment mono-).
2. after to soil treatment, in plantation, use the organic farm manure of microorganism, soil is carried out to organic improving, increase content and the fertility of probiotic bacterium in soil, form good rhizospheric environment and abundant trophoplasm, increase plant root health, improve Root Absorption ability, improve via plant immunity, prevent disease (implementation method refers to embodiment bis-).Microbial organic fertilizer 2000 ~ 2500kg is used on every mu of ground of general strawberry cultivating.
3. microorganism is added irrigation, is to supplement root probiotic bacterium and trophoplasm, adds compound probiotic liquid (implementation method refers to embodiment tri-) when irrigating.
4. foliage-spray, increases the synthetic of plant chlorophyll, urges and makes blade face light compositing, improves disease-resistant, insect-prevention ability, and improves the trophoplasm (implementation method refers to embodiment tetra-) of fruit.
5. expelling parasite is sprayed on ground, prevents external worm, the erosion (implementation method refer to embodiment five) of bacterium to Strawberry Seedlings from ground.
6. air atomizing sterilizing insect-killing, can suppress to kill airborne harmful microorganism, pre-preventing disease and pest (implementation method refers to embodiment six).
plantation Application Example
Embodiment 1. soil sterilizings are administered
The corrupt improvement method in plantation soil
If soil does not have corruption and causes root rot, do not need to carry out this operation.Also can before seedling under strawberry, carry out this operation.
1. soil treatment is used and is closed probiotic bacterium stoste containing milk-acid bacteria, yeast, actinomycetes bacterium, photosynthetic bacterium, genus bacillus etc.: every 100 square meters are used after 20 times of 5kg compound probiotic stoste dilute with waters, and available atomizer is evenly sprayed at will be administered on soil.
2. compound probiotic is evenly sprayed at and will be administered behind soil, with rotary cultivator, turn over one time, herbaceous plant is planted the 15 ~ 20cm that turns over, 30cm is ploughed deeply in xylophyta plantation, and then spray a milk-acid bacteria, laterally turn over once with rotary cultivator, then spray hug after compound probiotic flat.Unwanted bacteria major part after for some time in soil is killed, and recovers gradually the balance of nature of soil.
This method with the following Examples 3 method use subsequently, also have good effect.
Embodiment 2. compost are produced
Microbial organic fertilizer making method
1. compost is used containing compound probiotic stostes such as milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus.Usage ratio is 5% to be the compound probiotic stoste that compost per ton is used 50kg to contain milk-acid bacteria, spreads by atomizers spray, and compost material high humidity I is with suitable thin up probiotic bacterium stoste according to circumstances, to guarantee the normal fermentation of compost.
2. adopt artificial or mechanical mixing type to add bacterium stirring means: to turn over the loam that spreads thin layer, and then evenly sprinkle compound probiotic and stir, turn over subsequently broadcast application native while spread bacterium.Turn over and shed after bacterium finishes, compost bottom margin, hand mixing again, spills to dunghill middle and upper part being uniformly distributed in the hope of probiotic bacterium in dunghill.Then broken muck bat block dunghill surface, cover one deck plastic cloth and carry out fermentation maturity.
If conditions permit can be carried out compost by following formula: 1 cube of 1 cube of chicken manure, cow dung (also can be used fallen leaves stalk to replace or add stalk, but must be ground into 5-10mm left and right size), compound probiotic 5%, sugared slag 5%, ground phosphate rock 3%, peat composed of rotten mosses powder 3%, also can add the trace element of little amount 0.5-1%, as magnesia (magnesium oxide), iron ore powder and manganese ore powder etc., the about 850kg of finished product fertilizer making, humidity is pinched the degree that can just roll into a ball with hand, approximately 50~65% left and right.Use stirrer can make to spray milk-acid bacteria and add other attached material more even, be more conducive to fertilizer fermentation, shorten fermentation time etc.
3. the weather of temperature more than 20 ℃, more than covering film anaerobic fermentation temperature rise to 55 ~ 60 ℃, maintains 2 ~ 3 days and can open afterwards fertile heap, carries out secondary compound probiotic and sprays compost treatment.Turn over one deck loam and spray a probiotic bacterium, concrete consumption is consumption 5-10kg per ton, then air storage.After 2 ~ 3 days, carry out again a turning, mend bacterium, after air storage for some time, can use (as conditions permit, can turning in every 3 days mend bacterium once, be conducive to becoming thoroughly decomposed of fertilizer).
4. at the beginning of compost is preferably in annual 4 months, start, weather temperature raises, and processes and can do base fertilizer use in 1-2 month.
If 5. use the finished product fertilizer of buying as base fertilizer, the compound probiotic that can be directly adds 2kg to contain milk-acid bacteria etc. according to every 100kg fertilizer, adds water 5kg to stir, and applies to plant in soil and makees base fertilizer.
6. in order to guarantee the effect of compost, not mixed with the series products of other starters while using this product.
Embodiment 3. fills with radical cure reason
Compound probiotic adds irrigation method
1. filling with root adds: before planting Strawberry Seedlings, containing 500~1000 times of the compound probiotic dilute with waters such as milk-acid bacteria, yeast, nitrobacteria, genus bacillus, 200ml diluent is used in hole, each cave, water is planted into Strawberry Seedlings after oozing.Then plant upper Strawberry Seedlings, can promote like this growth of strawberry root system, suppress the harmful bacteria breeding in soil, when strawberry is grown seedlings, also can according to said method use.
2. drip irrigation is added: in the time of drip irrigation, be added in water, be directly penetrated into root.Each booth (400 square meter) is used 1000ml compound probiotic stoste to be added on direct drip irrigation in water at every turn, and every two weeks adds compound probiotic drip irrigation once.
Embodiment 4. foliage-sprays
Compound probiotic foliage-spray method
Foliage-spray: just can carry out cauline leaf after strawberry " seedling-slowing stage " and spray the compound probiotic of dilution containing photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus etc.Milk-acid bacteria in probiotic bacterium can increase the effect of light compositing bacterium, and light compositing bacterium can directly promote the photosynthesis of plant.The compound probiotic of dilution is in proportion sprayed on blade, produces a large amount of chlorophyll, guarantee luxuriance of vegetation and health.It is the absorption that plant stem-leaf has additional nutrients that the metabolic activity of compound probiotic can provide amino acid and various VITAMIN, Promoting plant growth.And can play the effect (full-bloom stage can not spray) of sterilization, and after result, still can spray, according to circumstances can within every 10 days, spray once.
Embodiment 5. ground deinsectization expelling parasite processing
Compound probiotic ground spraying method
Use contains the compound probiotic stostes such as milk-acid bacteria, yeast, genus bacillus, in the diameter range of 1~2 meter around seedling, with the consumption of 50~100 milliliters of every square meters, sprays, and can strengthen usage quantity according to disease and insect amount.
Start weekly spray once, gradually reduce, until situation alleviate or disappear after stop.According to the degree of disease and pest, determine the sprinkling amount of compound probiotic and spray frequency.
Embodiment 6. air atomizing sterilizing insect-killings
Compound probiotic air atomizing
Because strawberry mostly is greenhouse gardening, airborne harmful germ and spore thereof cannot be removed in time, can cause the cross infection of disease in booth enclosed space.Use containing compound probiotic stostes such as milk-acid bacteria, yeast, genus bacillus and carry out after atomization air to spray, atomization sprays once weekly.This method sprays with ground and sprays in conjunction with cauline leaf, mainly the mushroom spores such as airborne Powdery Mildew is suppressed, and suppresses to kill in air, the bacterium on native table, blade face and fruit and acarid etc., improves plant disease-resistant ability.
comparison of experiment results
By buying conventional compound probiotic microbial inoculum in contrast on market, commercially available prod 1 is the probiotic bacterium that Beijing company sells, and contains take photosynthetic bacteria, milk-acid bacteria, yeast as main probiotic bacterium and meta-bolites; The compound probiotic take milk-acid bacteria as master that sell for bio tech ltd, Yichuan commercially available prod 2.
Experiment is beauty by strawberry cultivars.
Compound probiotic (experimental group 1), the commercially available prod 1(experimental group 2 of under equal experiment condition, with our company, producing), commercially available prod 2(experimental group 3) and do not use compound probiotic (control group) to plant processing to strawberry, in the on all four situation of Cultivate administration mode, strawberry fruit is detected, and result is as follows:
1. experimental group 1 Yield of Strawberry improves 17.2% compared with control group, compared with experimental group 2, improves 10.4%, compared with experimental group 3, improves 8.9%
2. strawberry fruit is scarlet compared with control group outward appearance, and size is homogeneous more, and single fruit weight is on average high by 5.7% compared with control group, compared with experimental group 1, improves 3.5%, compared with experimental group 2, improves 2.7%.
3. experimental group Powdery Mildew obviously reduces compared with control group.
4. the phase experimental group of bearing fruit on average extends 5 ~ 7 days compared with control group.
5. strawberry mouthfeel is stronger fragrant and sweet compared with experimental group 2, experimental group 3, control group, quality has clear improvement, protein, soluble solid, Vc content are all significantly increased, detailed data sees the following form, table 1 is experimental group 1 and control group Quality Detection contrast table, table 2 is experimental group 1 and experimental group 2 Quality Detection contrast tables, and table 3 is experimental group 1 and experimental group 3 Quality Detection contrast tables.
Table 1 experimental group 1 and the contrast of control group Quality Detection
2 groups of Quality Detection contrasts of table 2 experimental group 1 and experiment
Table 3 experimental group 1 and experimental group 3 Quality Detection contrasts

Claims (10)

1. for a compound probiotic for strawberry cultivating, mainly by milk-acid bacteria, formed.
2. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that soil remediation is used, containing milk-acid bacteria, yeast, actinomycetes bacterium, photosynthetic bacterium, genus bacillus etc.
3. probiotic bacterium claimed in claim 1, it is characterized in that can be used for making the compound probiotic that microbial-bacterial fertilizer is used: containing milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus etc.
4. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that pouring is used: containing milk-acid bacteria, yeast, nitrobacteria, genus bacillus etc.
5. probiotic bacterium claimed in claim 1, it is characterized in that can be used for cauline leaf and sprays the compound probiotic of use: containing photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus etc.
6. probiotic bacterium claimed in claim 1, it is characterized in that can be used for ground and sprays the compound probiotic of use: containing milk-acid bacteria, yeast, genus bacillus etc.
7. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that air atomizing degerming is used: containing milk-acid bacteria, yeast, genus bacillus etc.
8. the application of probiotic bacterium in strawberry cultivating described in claim 1-7, is characterized in that being produced, being filled with by soil sterilizing improvement, compost the modes such as radical cure reason, foliage-spray, ground deinsectization expelling parasite processing and air atomizing sterilizing insect-killing and applies.
9. the preparation method of probiotic bacterium described in claim 1-7, is characterized in that: produce first respectively milk-acid bacteria, yeast, subtilis, photosynthetic bacterium, actinomycetic fermented liquid, then above-mentioned fermented liquid is composited by weight.
10. the compound probiotic preparation method described in claims 9, is characterized in that:
The production method of described milk-acid bacteria is: in 1kg water, add brown sugar 0.05~1.0kg, be configured to after solution, inoculum size with 1%~5% adds lactic acid bacteria culturers, sealed fermenting in anaerobic fermentation tank, 33~38 ℃ of controlled fermentation temperature, fermentation time 3~7 days, the liquid spawn having activated is added to second order fermentation tank sealed fermenting, second order fermentation liquid proportioning is: molasses 10%, amino acid 0.1%~0.5%, 33~38 ℃ of VITAMIN 0.05% ~ 1% controlled fermentation temperature, fermentation time 3~7 days, inoculum size 5%~10%, between yeast phase, constantly add molasses, treat that bacterial classification concentration reaches 300,000,000/ml, pH is 4.2 o'clock, stop fermentation,
Described saccharomycetic production method is:
By being inoculated into bacterial classification in yeast, expand in fermented liquid, the formula of fermented liquid is: 50g/L, urea (NH 4) 2sO 41g/L, KH 2pO 42.5g/L Na 2hPO 40.5g/L, MgSO 41g/L, FeSO 40.1g/L, yeast extract paste 0.5g/L, rose-bengal 0.03g/L, pH4.5-5.0, sealed fermenting in aerobic fermentation tank, controlled fermentation temperature 25-28 ℃, fermentation time 2-3 days, the liquid spawn having activated is added to the fermentation of secondary aerobic fermentation tank, second order fermentation liquid proportioning is: soybean sprout 100g/L, glucose 50g/L, pH nature, controlled fermentation temperature 25-28 ℃, fermentation time 3~5 days, inoculum size 5%~10%, constantly adds glucose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation;
The production method of described subtilis is: subtilis is seeded in substratum, culture medium prescription is: peptone 1 %, yeast extract 0. 5 %, sodium-chlor 1 %, nature pH, is placed in 37 ℃, seed fermentation tank and cultivates after 1-2 days, access fermention medium, culture medium prescription is: sucrose 1 %, peptone 1 %, Sodium phosphate dibasic 0. 2 %, SODIUM PHOSPHATE, MONOBASIC 0. 2 %, in natural pH, inoculum size 5%~10%, 35~37 ℃ of temperature, constantly add sucrose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation;
The production method of described photosynthetic bacterium is: photosynthetic bacterium is seeded in yeast extract paste protein culture medium, and basic recipe is: CaCL 20.3g, MgSO 47H 2o 0.5g, yeast extract paste 3g, peptone 3g, distilled water 1000ml, adjusts pH 6.8, in culture temperature: 25 ℃~30 ℃, intensity of illumination: 2000LX~5000LX, lower cultivation 3 days, afterwards in aerobic fermentation tank with 15% inoculum size, 25 ℃~30 ℃ of temperature, intensity of illumination: 2000LX~5000LX, cultivates under the condition of pH:7.5~8.5, and in fermenting process, results of regular determination pH also carrys out neutralise broth with 150g/L Sulfothiorine, until bacterial classification concentration, reach 300,000,000/ml when above, stop fermentation;
Described actinomycetic production method is: bacterial classification is inoculated in No. 1 substratum of Gao Shi (GA), and main component is: Zulkovsky starch 20g, KNO 31g, K 2hPO 40.5g, MgSO 47H 2o 0.5g, NaCl 0.5g, FeSO 47H 2o 0.01g, pH=7.4-7.6, water 1000ml enlarged culturing is controlled 28~30 ℃ of temperature in same medium with 5%~10% inoculum size afterwards in aerobic fermentation tank, and pH7.0-7.6 fermentation culture reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation;
The production method of described nitrobacteria is: nitrobacteria is seeded in substratum, and culture medium prescription is: ammonium sulfate 0.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, SODIUM PHOSPHATE, MONOBASIC 1g, magnesium sulfate 0.03g, CaCl 27.5g, distilled water 1000ml, in pH 7.5~8.0, enlarged culturing 3 days under 28~30 ℃ of conditions of temperature, with 5%~10% inoculum size, in the aerobic large fermentation tank of same medium, control 28~30 ℃ of temperature afterwards, pH7.5-8.0 fermentation culture, reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation.
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Application publication date: 20140416