CN108713449A - A kind of cultural method of Poria cocos - Google Patents
A kind of cultural method of Poria cocos Download PDFInfo
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- CN108713449A CN108713449A CN201810563143.1A CN201810563143A CN108713449A CN 108713449 A CN108713449 A CN 108713449A CN 201810563143 A CN201810563143 A CN 201810563143A CN 108713449 A CN108713449 A CN 108713449A
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- poria cocos
- bacterium
- vitamin
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- culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention discloses a kind of cultural methods of Poria cocos, it cultivates Poria cocos according to the following steps：Culture base-material preparation, the preparation of bacterium material, site preparation, is laid with bacterium material at the culture of culture base-material sterilizing, Poria cocos cultivation bacterium.The Poria cocos cultivation bacterium, which is cultivated, is：Poria cocos mother bacterium is inoculated into cultivating bacteria bag, and cultivating bacteria bag is placed in sterile environment, is cultivated at 28 DEG C and obtains within 30 days mycelium to get Poria cocos cultivation bacterium.The present invention provides a kind of cultural method of Poria cocos, has many advantages, such as that bacterium germination is fast, inoculation survival rate is high, growth cycle is short, yield is high, quality is good, can be widely used in large-scale tuckahoe production and culture.
Description
Technical field
The invention belongs to fungi artificial cultivation technique fields, and in particular to a kind of cultural method of Poria cocos.
Background technology
Poria cocos is China's tradition parts of generic medicinal plants and dietotherapeutic resource, is widely used in tcm clinical practice formula, Chinese patent drug and
Healthy food material.
The wild resource of China Poria cocos bacterium is rare, and is distributed fragmentary dispersion, it is difficult to excavate.For a long time, Poria cocos commodity by
Producing region peasant, which cultivates, to be provided.
The theoretical foundation of Poria cocos cultivation：(1) life habit of Poria cocos bacterium：It is sought using cellulose, hemicellulose as nutrient source rotten
Life；(2) formation mechenism of sclerotium：It is to be formed " mycelium agglomerate ", then turned by aggregation, knot by a large amount of mycelium
Change forms sclerotium " former base ", and the nutriment of mycelium conversion is received, accumulated with this, gradually increases, develops.
But Poria cocos requires harshness to growing environment, the artificial cultivation of Poria cocos is by landform and natural climate item in the prior art
Part influences big, is suitble to the time that the region of artificial cultivation Poria cocos is smaller, meets Poria cocos the best growing condition in 1 year shorter, spins out
Production cycle, the economic benefit for influencing artificial cultivation Poria cocos.
Therefore, it there is an urgent need to a kind of efficient, high yield, the artificial Poria cocos cultivation technique to suit large area to popularize, is planted with shortening
The time limit is trained, realizes facility Efficient Cultivation.
Through the retrieval discovery to the prior art, Chinese invention patent application 200910094134.3（Publication date in July, 2009
29 days）A kind of cultural method of high-efficiency environmental-protection Tuckahoe is disclosed, method is：A. 3-10 centimetres of pine tree side shoot of diameter is selected to be made
Round or polygon slice；It takes out and drains away the water after being impregnated 12 hours in water, control water content to 70%：It is subsequently filled into edible
In bacteria cultivation bag；Normal-pressure sterilization 12 hours sterilizes 2 hours at a temperature of 120 DEG C, when temperature is down to 25 DEG C in bag, is connect
Kind；It is spare after culture 30 days under the conditions of 25 DEG C of temperature；B. selection culture juggle digs cellar for storing things, disinfection；C. culture juggle is put into and is disappeared
In being stored after poison, take out the tuckahoe cultivated species that prepare in a, with stainless steel nail by cultigen slice nail in culture juggle side,
Section；D. vaccinated culture juggle is covered tightly into simultaneously 20 centimetres of earthing with degradation plastic film；E. it cultivates and manages according to conventional Poria cocos
Reason extremely harvests.This method has the advantages that easy to operate, bacterium germination is fast, inoculation survival rate is high, Soil Environmental Pollution is reduced, can be extensively
For large-scale tuckahoe production and culture.But there is low output, growth cycle is long the problems such as.
And Chinese invention patent application 201510935551.1（Publication date on April 20th, 2016）It is high to disclose a kind of Poria cocos
Imitate cultural method, including following operating procedure：1 selection last year cultivation body is full, color of the leather is pale red, has crackle, is yellowish pink pure white, thin
Tender fresh Poria cocos is as kind of a Siberian cocklebur；2 10-15 days behind cellar for storing things, interweave with forming the mycelium of white velveteen shape in mycelium, there is liquid under strain
Change droplet as interface position；The kind Siberian cocklebur chosen is drawn with scraper into meat and is rotated a little, 100-120 grams of fritter is broken into, with
It secretes the one side more than yellowish-brown dewdrop as inoculating surfaces, the interface mycoderm of selection is peeled off with bamboo knife, face paste is contacted with Siberian cocklebur block
Tight section wood interface makes Siberian cocklebur block closely agree with section wood, compact with soil, then earthing restores Siberian cocklebur cellar for storing things；Dredge gutter.This method is direct
Enter the three phases of Poria cocos growth and development by the Poria cocos Hyphae Growth stage, the opposite growth course for shortening Poria cocos makes original
Material mycelia nutrition is fully used, and to improve the utilization rate of pine, realizes that Poria cocos cultivation production is rich of fine quality.But due to not into
The processing of row sterilizing to influence the growth of Poria cocos, and then influences the quality of Poria cocos.
Based on this, it is necessary to a kind of cultural method of Poria cocos is provided, to solve problems of the prior art.
Invention content
The present invention in view of the above shortcomings of the prior art, provides a kind of cultural method of Poria cocos, have bacterium germination is fast, inoculation at
The advantages that motility rate is high, growth cycle is short, yield is high, quality is good, can be widely used in large-scale tuckahoe production and culture.
To achieve the goals above, the technical solution adopted by the present invention is：
A kind of cultural method of Poria cocos, includes the following steps：
(1) culture base-material is prepared：By 84-88 parts of sawdust, 12-16 parts of wheat bran, 5-10 parts of pine sawdust, 3-5 parts of campanulaceae stem, water 55-
60 parts, by the proportioning mixing of above-mentioned parts by weight, are sealed by fermentation 5-8 days, the 0.1-0.3% relative to sawdust dry mass are then added
Multi-vitamins and 0.05-0.1% relative to sawdust dry mass trace element, be uniformly mixed to get culture base-material；
（2）Culture base-material sterilizing：By step（1）The culture base-material of preparation is put into Polythene Bag, and cultivating bacteria bag is made, and
Carry out sterilizing；
（3）Poria cocos cultivates bacterium culture：Poria cocos mother bacterium is inoculated into cultivating bacteria bag, and cultivating bacteria bag is placed in sterile environment
It is interior, it is cultivated at 28 DEG C and obtains within 30 days mycelium to get Poria cocos cultivation bacterium；
（4）Bacterium material prepares：With deciduous species timber segment, multiple fish scale mouths are chopped in timber section, in fish scale mouth and timber section two
Termination kind Poria cocos cultivates bacterium；
（5）Site preparation：Select weather nice and cool, the sparse woods hillside of 700-800 meters of height above sea level, arable land, digging is 80 centimeters wide, deep 30 centimeters
Long bar ditch, bottom of trench spread 5 centimeters of thick matrix；
（6）It is laid with bacterium material：Bacterium material strip prepared by step (4) is laid with, and timber section is placed in below soil, in timber section
Poria mycelium is grown naturally in the soil.
Preferably, step（1）In, the sawdust is in masson pine, Pinus taiwanesis, Japanese red pine, black pine, larch, Chinese fir
One or more.
Preferably, step（1）In, the multi-vitamins include vitamin A, vitamine D3, vitamin E, vitamin
B1 and vitamin B6, and every kilogram of multi-vitamins contain：Vitamin A 15000000IU, vitamine D3 6000000, dimension life
Plain E 100000IU, vitamin B1 12000mg, vitamin B6 16000mg and surplus are carrier defatted rice bran.
Preferably, step（1）In, the trace mineral supplement formula is that every kilogram of trace mineral supplement contains：
Copper 25mg, iron 400mg, zinc 500mg, manganese 500mg, iodine 15mg, selenium 1.5mg, cobalt 10mg and surplus are carrier zeolite.
Preferably, step（2）In, the method for the sterilizing：By cultivating bacteria bag be placed in 0.15MPa pressure, 120 DEG C
Lower sterilizing 2h, or with 100 DEG C of flowing steam steam sterilizing 5-7h.
Preferably, the volume of the cultivating bacteria bag is Φ 15cm × 35cm, it is 650g per packed culture doses
Preferably, step（3）In, inoculum concentration when culture Poria cocos cultivates bacterium is：Match culture medium according to Poria cocos mother bacterium 6ml
The envelope-bulk to weight ratio of material 330-400g is inoculated with.
Preferably, step（4）In, further include carrying out air-drying sterilization processing to timber section.
Preferably, timber section is air-dried by nature solarization, timber section water content is set to be less than 15%, then by timber section
It is put into wet sterilization pot, carries out steam sterilization；
Preferably, step（6）In, it is covered in the thickness of soil above timber section and is no more than 4cm.
The cultural method of Poria cocos of the present invention achieves following beneficial technique effect：
(1) cultivation of the invention uses dedicated culture base-material, plays uniform moisturizing, uniformly ventilative and nutrition work
With, for Poria cocos mycelium germination, Poria cocos kind growth create it is good, stablize growing environment.
（2）The present invention uses advanced cultivation technique, has power consumption few, and human cost is low, not only contributes to improve Poria cocos
Production yields, and it is advantageously ensured that product quality.
（3）The present invention makes it become bulk and soft by carrying out fermentation process to sawdust, wheat bran, pine sawdust, campanulaceae stem
Soft, nutritional ingredient discharges, and is more favorable for the growth of Poria cocos, and to improve, inoculation survival rate is high, shorten the period and increases production
Amount.
（4）Multi-vitamins and trace element that the present invention adds can effectively facilitate the development of mycelia, improve inoculation
Survival rate.
（5）The entire cultivation of the present invention uses environmentally protective Cultivating techniques, not can cause environmental pollution, and can
To effectively improve the yield and quality of Poria cocos.
Specific implementation mode
Technical scheme of the present invention is further described below in conjunction with specific embodiment, the content of the embodiment is only
For illustrating the present invention, it is not meant to define protection scope of the present invention.
Embodiment 1
The cultural method of the Poria cocos of the present embodiment, includes the following steps：
(1) culture base-material is prepared：84 parts of sawdust, 12 parts of wheat bran, 5 parts of pine sawdust, 3 parts of campanulaceae stem, 55 parts of water are pressed into above-mentioned weight
Number proportioning mixing, be sealed by fermentation 5-8 days, be then added relative to sawdust dry mass 0.1% multi-vitamins and relatively
In 0.05% trace element of sawdust dry mass, it is uniformly mixed to get culture base-material；
（2）Culture base-material sterilizing：By step（1）The culture base-material of preparation is put into Polythene Bag, and cultivating bacteria bag is made, and
Carry out sterilizing；
（3）Poria cocos cultivates bacterium culture：Poria cocos mother bacterium is inoculated into cultivating bacteria bag, and cultivating bacteria bag is placed in sterile environment
It is interior, it is cultivated at 28 DEG C and obtains within 30 days mycelium to get Poria cocos cultivation bacterium；
（4）Bacterium material prepares：With deciduous species timber segment, multiple fish scale mouths are chopped in timber section, in fish scale mouth and timber section two
Termination kind Poria cocos cultivates bacterium；
（5）Site preparation：Select weather nice and cool, the sparse woods hillside of 700-800 meters of height above sea level, arable land, digging is 80 centimeters wide, deep 30 centimeters
Long bar ditch, bottom of trench spread 5 centimeters of thick matrix；
（6）It is laid with bacterium material：Bacterium material strip prepared by step (4) is laid with, and timber section is placed in below soil, in timber section
Poria mycelium is grown naturally in the soil.
Wherein, step（1）In, the sawdust is selected from masson pine, Pinus taiwanesis, and one in Japanese red pine, black pine, larch, Chinese fir
Kind is several.
Wherein, step（1）In, the multi-vitamins include vitamin A, vitamine D3, vitamin E, vitamin B1 and
Vitamin B6, and every kilogram of multi-vitamins contain：Vitamin A 15000000IU, vitamine D3 6000000, vitamin E
100000IU, vitamin B1 12000mg, vitamin B6 16000mg and surplus are carrier defatted rice bran.
Wherein, step（1）In, the trace mineral supplement formula is that every kilogram of trace mineral supplement contains：Copper
25mg, iron 400mg, zinc 500mg, manganese 500mg, iodine 15mg, selenium 1.5mg, cobalt 10mg and surplus are carrier zeolite.
Wherein, step（2）In, the method for the sterilizing：Cultivating bacteria bag is placed in 0.15MPa pressure, is gone out at 120 DEG C
Bacterium 2h, or with 100 DEG C of flowing steam steam sterilizing 5-7h.
Wherein, the volume of the cultivating bacteria bag is Φ 15cm × 35cm, is 650g per packed culture doses
Wherein, step（3）In, inoculum concentration when culture Poria cocos cultivates bacterium is：Match culture base-material according to Poria cocos mother bacterium 6ml
The envelope-bulk to weight ratio of 330-400g is inoculated with.
Wherein, step（4）In, further include carrying out air-drying sterilization processing to timber section.
Wherein, timber section is air-dried by nature solarization, so that timber section water content is less than 15%, is then put into timber section
In wet sterilization pot, steam sterilization is carried out；
Wherein, step（6）In, it is covered in the thickness of soil above timber section and is no more than 4cm.
Embodiment 2
The cultural method of the Poria cocos of the present embodiment, includes the following steps：
(1) culture base-material is prepared：88 parts of sawdust, 16 parts of wheat bran, 10 parts of pine sawdust, 5 parts of campanulaceae stem, 60 parts of water are pressed into above-mentioned weight
Number proportioning mixing, be sealed by fermentation 5-8 days, be then added relative to sawdust dry mass 0.3% multi-vitamins and relatively
In 0.1% trace element of sawdust dry mass, it is uniformly mixed to get culture base-material；
（2）Culture base-material sterilizing：By step（1）The culture base-material of preparation is put into Polythene Bag, and cultivating bacteria bag is made, and
Carry out sterilizing；
（3）Poria cocos cultivates bacterium culture：Poria cocos mother bacterium is inoculated into cultivating bacteria bag, and cultivating bacteria bag is placed in sterile environment
It is interior, it is cultivated at 28 DEG C and obtains within 30 days mycelium to get Poria cocos cultivation bacterium；
（4）Bacterium material prepares：With deciduous species timber segment, multiple fish scale mouths are chopped in timber section, in fish scale mouth and timber section two
Termination kind Poria cocos cultivates bacterium；
（5）Site preparation：Select weather nice and cool, the sparse woods hillside of 700-800 meters of height above sea level, arable land, digging is 80 centimeters wide, deep 30 centimeters
Long bar ditch, bottom of trench spread 5 centimeters of thick matrix；
（6）It is laid with bacterium material：Bacterium material strip prepared by step (4) is laid with, and timber section is placed in below soil, in timber section
Poria mycelium is grown naturally in the soil.
Wherein, step（1）In, the sawdust is selected from masson pine, Pinus taiwanesis, and one in Japanese red pine, black pine, larch, Chinese fir
Kind is several.
Wherein, step（1）In, the multi-vitamins include vitamin A, vitamine D3, vitamin E, vitamin B1 and
Vitamin B6, and every kilogram of multi-vitamins contain：Vitamin A 15000000IU, vitamine D3 6000000, vitamin E
100000IU, vitamin B1 12000mg, vitamin B6 16000mg and surplus are carrier defatted rice bran.
Wherein, step（1）In, the trace mineral supplement formula is that every kilogram of trace mineral supplement contains：Copper
25mg, iron 400mg, zinc 500mg, manganese 500mg, iodine 15mg, selenium 1.5mg, cobalt 10mg and surplus are carrier zeolite.
Wherein, step（2）In, the method for the sterilizing：Cultivating bacteria bag is placed in 0.15MPa pressure, is gone out at 120 DEG C
Bacterium 2h, or with 100 DEG C of flowing steam steam sterilizing 5-7h.
Wherein, the volume of the cultivating bacteria bag is Φ 15cm × 35cm, is 650g per packed culture doses
Wherein, step（3）In, inoculum concentration when culture Poria cocos cultivates bacterium is：Match culture base-material according to Poria cocos mother bacterium 6ml
The envelope-bulk to weight ratio of 330-400g is inoculated with.
Wherein, step（4）In, further include carrying out air-drying sterilization processing to timber section.
Wherein, timber section is air-dried by nature solarization, so that timber section water content is less than 15%, is then put into timber section
In wet sterilization pot, steam sterilization is carried out；
Wherein, step（6）In, it is covered in the thickness of soil above timber section and is no more than 4cm.
Embodiment 3
The cultural method of the Poria cocos of the present embodiment, includes the following steps：
(1) culture base-material is prepared：By 96 parts of sawdust, 14 parts of wheat bran, 7.5 parts of pine sawdust, 4 parts of campanulaceae stem, 57 parts of water by above-mentioned heavy
Number proportioning mixing is measured, is sealed by fermentation 5-8 days, 0.2% multi-vitamins and phase relative to sawdust dry mass is then added
For 0.07% trace element of sawdust dry mass, it is uniformly mixed to get culture base-material；
（2）Culture base-material sterilizing：By step（1）The culture base-material of preparation is put into Polythene Bag, and cultivating bacteria bag is made, and
Carry out sterilizing；
（3）Poria cocos cultivates bacterium culture：Poria cocos mother bacterium is inoculated into cultivating bacteria bag, and cultivating bacteria bag is placed in sterile environment
It is interior, it is cultivated at 28 DEG C and obtains within 30 days mycelium to get Poria cocos cultivation bacterium；
（4）Bacterium material prepares：With deciduous species timber segment, multiple fish scale mouths are chopped in timber section, in fish scale mouth and timber section two
Termination kind Poria cocos cultivates bacterium；
（5）Site preparation：Select weather nice and cool, the sparse woods hillside of 700-800 meters of height above sea level, arable land, digging is 80 centimeters wide, deep 30 centimeters
Long bar ditch, bottom of trench spread 5 centimeters of thick matrix；
（6）It is laid with bacterium material：Bacterium material strip prepared by step (4) is laid with, and timber section is placed in below soil, in timber section
Poria mycelium is grown naturally in the soil.
Wherein, step（1）In, the sawdust is selected from masson pine, Pinus taiwanesis, and one in Japanese red pine, black pine, larch, Chinese fir
Kind is several.
Wherein, step（1）In, the multi-vitamins include vitamin A, vitamine D3, vitamin E, vitamin B1 and
Vitamin B6, and every kilogram of multi-vitamins contain：Vitamin A 15000000IU, vitamine D3 6000000, vitamin E
100000IU, vitamin B1 12000mg, vitamin B6 16000mg and surplus are carrier defatted rice bran.
Wherein, step（1）In, the trace mineral supplement formula is that every kilogram of trace mineral supplement contains：Copper
25mg, iron 400mg, zinc 500mg, manganese 500mg, iodine 15mg, selenium 1.5mg, cobalt 10mg and surplus are carrier zeolite.
Wherein, step（2）In, the method for the sterilizing：Cultivating bacteria bag is placed in 0.15MPa pressure, is gone out at 120 DEG C
Bacterium 2h, or with 100 DEG C of flowing steam steam sterilizing 5-7h.
Wherein, the volume of the cultivating bacteria bag is Φ 15cm × 35cm, is 650g per packed culture doses
Wherein, step（3）In, inoculum concentration when culture Poria cocos cultivates bacterium is：Match culture base-material according to Poria cocos mother bacterium 6ml
The envelope-bulk to weight ratio of 330-400g is inoculated with.
Wherein, step（4）In, further include carrying out air-drying sterilization processing to timber section.
Wherein, timber section is air-dried by nature solarization, so that timber section water content is less than 15%, is then put into timber section
In wet sterilization pot, steam sterilization is carried out；
Wherein, step（6）In, it is covered in the thickness of soil above timber section and is no more than 4cm.
Embodiment 4
The cultural method of the Poria cocos of the present embodiment, includes the following steps：
(1) culture base-material is prepared：By 85 parts of sawdust, 13 parts of wheat bran, 6 parts of pine sawdust, 3.5 parts of campanulaceae stem, 56 parts of water by above-mentioned heavy
Measure number proportioning mixing, be sealed by fermentation 5-8 days, be then added relative to sawdust dry mass 0.15% multi-vitamins and
0.06% trace element relative to sawdust dry mass is uniformly mixed to get culture base-material；
（2）Culture base-material sterilizing：By step（1）The culture base-material of preparation is put into Polythene Bag, and cultivating bacteria bag is made, and
Carry out sterilizing；
（3）Poria cocos cultivates bacterium culture：Poria cocos mother bacterium is inoculated into cultivating bacteria bag, and cultivating bacteria bag is placed in sterile environment
It is interior, it is cultivated at 28 DEG C and obtains within 30 days mycelium to get Poria cocos cultivation bacterium；
（4）Bacterium material prepares：With deciduous species timber segment, multiple fish scale mouths are chopped in timber section, in fish scale mouth and timber section two
Termination kind Poria cocos cultivates bacterium；
（5）Site preparation：Select weather nice and cool, the sparse woods hillside of 700-800 meters of height above sea level, arable land, digging is 80 centimeters wide, deep 30 centimeters
Long bar ditch, bottom of trench spread 5 centimeters of thick matrix；
（6）It is laid with bacterium material：Bacterium material strip prepared by step (4) is laid with, and timber section is placed in below soil, in timber section
Poria mycelium is grown naturally in the soil.
Wherein, step（1）In, the sawdust is selected from masson pine, Pinus taiwanesis, and one in Japanese red pine, black pine, larch, Chinese fir
Kind is several.
Wherein, step（1）In, the multi-vitamins include vitamin A, vitamine D3, vitamin E, vitamin B1 and
Vitamin B6, and every kilogram of multi-vitamins contain：Vitamin A 15000000IU, vitamine D3 6000000, vitamin E
100000IU, vitamin B1 12000mg, vitamin B6 16000mg and surplus are carrier defatted rice bran.
Wherein, step（1）In, the trace mineral supplement formula is that every kilogram of trace mineral supplement contains：Copper
25mg, iron 400mg, zinc 500mg, manganese 500mg, iodine 15mg, selenium 1.5mg, cobalt 10mg and surplus are carrier zeolite.
Wherein, step（2）In, the method for the sterilizing：Cultivating bacteria bag is placed in 0.15MPa pressure, is gone out at 120 DEG C
Bacterium 2h, or with 100 DEG C of flowing steam steam sterilizing 5-7h.
Wherein, the volume of the cultivating bacteria bag is Φ 15cm × 35cm, is 650g per packed culture doses
Wherein, step（3）In, inoculum concentration when culture Poria cocos cultivates bacterium is：Match culture base-material according to Poria cocos mother bacterium 6ml
The envelope-bulk to weight ratio of 330-400g is inoculated with.
Wherein, step（4）In, further include carrying out air-drying sterilization processing to timber section.
Wherein, timber section is air-dried by nature solarization, so that timber section water content is less than 15%, is then put into timber section
In wet sterilization pot, steam sterilization is carried out；
Wherein, step（6）In, it is covered in the thickness of soil above timber section and is no more than 4cm.
Embodiment 5
The cultural method of the Poria cocos of the present embodiment, includes the following steps：
(1) culture base-material is prepared：By 87 parts of sawdust, 15 parts of wheat bran, 9 parts of pine sawdust, 4.5 parts of campanulaceae stem, 59 parts of water by above-mentioned heavy
Measure number proportioning mixing, be sealed by fermentation 5-8 days, be then added relative to sawdust dry mass 0.25% multi-vitamins and
0.09% trace element relative to sawdust dry mass is uniformly mixed to get culture base-material；
（2）Culture base-material sterilizing：By step（1）The culture base-material of preparation is put into Polythene Bag, and cultivating bacteria bag is made, and
Carry out sterilizing；
（3）Poria cocos cultivates bacterium culture：Poria cocos mother bacterium is inoculated into cultivating bacteria bag, and cultivating bacteria bag is placed in sterile environment
It is interior, it is cultivated at 28 DEG C and obtains within 30 days mycelium to get Poria cocos cultivation bacterium；
（4）Bacterium material prepares：With deciduous species timber segment, multiple fish scale mouths are chopped in timber section, in fish scale mouth and timber section two
Termination kind Poria cocos cultivates bacterium；
（5）Site preparation：Select weather nice and cool, the sparse woods hillside of 700-800 meters of height above sea level, arable land, digging is 80 centimeters wide, deep 30 centimeters
Long bar ditch, bottom of trench spread 5 centimeters of thick matrix；
（6）It is laid with bacterium material：Bacterium material strip prepared by step (4) is laid with, and timber section is placed in below soil, in timber section
Poria mycelium is grown naturally in the soil.
Wherein, step（1）In, the sawdust is selected from masson pine, Pinus taiwanesis, and one in Japanese red pine, black pine, larch, Chinese fir
Kind is several.
Wherein, step（1）In, the multi-vitamins include vitamin A, vitamine D3, vitamin E, vitamin B1 and
Vitamin B6, and every kilogram of multi-vitamins contain：Vitamin A 15000000IU, vitamine D3 6000000, vitamin E
100000IU, vitamin B1 12000mg, vitamin B6 16000mg and surplus are carrier defatted rice bran.
Wherein, step（1）In, the trace mineral supplement formula is that every kilogram of trace mineral supplement contains：Copper
25mg, iron 400mg, zinc 500mg, manganese 500mg, iodine 15mg, selenium 1.5mg, cobalt 10mg and surplus are carrier zeolite.
Wherein, step（2）In, the method for the sterilizing：Cultivating bacteria bag is placed in 0.15MPa pressure, is gone out at 120 DEG C
Bacterium 2h, or with 100 DEG C of flowing steam steam sterilizing 5-7h.
Wherein, the volume of the cultivating bacteria bag is Φ 15cm × 35cm, is 650g per packed culture doses
Wherein, step（3）In, inoculum concentration when culture Poria cocos cultivates bacterium is：Match culture base-material according to Poria cocos mother bacterium 6ml
The envelope-bulk to weight ratio of 330-400g is inoculated with.
Wherein, step（4）In, further include carrying out air-drying sterilization processing to timber section.
Wherein, timber section is air-dried by nature solarization, so that timber section water content is less than 15%, is then put into timber section
In wet sterilization pot, steam sterilization is carried out；
Wherein, step（6）In, it is covered in the thickness of soil above timber section and is no more than 4cm.
Comparative example 1
Using the cultural method of Chinese invention patent application 200910094134.3.
Comparative example 2
Using the cultural method of Chinese invention patent application 201510935551.1.
Test example
Embodiment 1-5 of the present invention and comparative example 1, the cultural method benefit of comparative example 2 are compared, the results are shown in Table 1.
Table 1
| Mu/member | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Comparative example 1 | Comparative example 2 |
| Cost | 14000 | 14000 | 14000 | 14000 | 14000 | 13000 | 12000 |
| Yield | 49000 | 49500 | 50500 | 50000 | 49500 | 35000 | 37000 |
| Benefit | 35000 | 35500 | 36500 | 36000 | 35500 | 22000 | 25000 |
As shown in Table 1, cultural method of the present invention in actual production compared to comparative example 1, comparative example 2, can be carried significantly
High economic benefit.
The creativeness of the present invention is mainly reflected in the following：
(1) cultivation of the invention uses dedicated culture base-material, plays uniform moisturizing, uniformly ventilative and nutrition work
With, for Poria cocos mycelium germination, Poria cocos kind growth create it is good, stablize growing environment.
（2）The present invention uses advanced cultivation technique, has power consumption few, and human cost is low, not only contributes to improve Poria cocos
Production yields, and it is advantageously ensured that product quality.
（3）The present invention makes it become bulk and soft by carrying out fermentation process to sawdust, wheat bran, pine sawdust, campanulaceae stem
Soft, nutritional ingredient discharges, and is more favorable for the growth of Poria cocos, and to improve, inoculation survival rate is high, shorten the period and increases production
Amount.
（4）Multi-vitamins and trace element that the present invention adds can effectively facilitate the development of mycelia, improve inoculation
Survival rate.
（5）The entire cultivation of the present invention uses environmentally protective Cultivating techniques, not can cause environmental pollution, and can
To effectively improve the yield and quality of Poria cocos.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of cultural method of Poria cocos, which is characterized in that include the following steps：
(1) culture base-material is prepared：By 84-88 parts of sawdust, 12-16 parts of wheat bran, 5-10 parts of pine sawdust, 3-5 parts of campanulaceae stem, water 55-
60 parts, by the proportioning mixing of above-mentioned parts by weight, are sealed by fermentation 5-8 days, the 0.1-0.3% relative to sawdust dry mass are then added
Multi-vitamins and 0.05-0.1% relative to sawdust dry mass trace element, be uniformly mixed to get culture base-material；
（2）Culture base-material sterilizing：By step（1）The culture base-material of preparation is put into Polythene Bag, and cultivating bacteria bag is made, and
Carry out sterilizing；
（3）Poria cocos cultivates bacterium culture：Poria cocos mother bacterium is inoculated into cultivating bacteria bag, and cultivating bacteria bag is placed in sterile environment
It is interior, it is cultivated at 28 DEG C and obtains within 30 days mycelium to get Poria cocos cultivation bacterium；
（4）Bacterium material prepares：With deciduous species timber segment, multiple fish scale mouths are chopped in timber section, in fish scale mouth and timber section two
Termination kind Poria cocos cultivates bacterium；
（5）Site preparation：Select weather nice and cool, the sparse woods hillside of 700-800 meters of height above sea level, arable land, digging is 80 centimeters wide, deep 30 centimeters
Long bar ditch, bottom of trench spread 5 centimeters of thick matrix；
（6）It is laid with bacterium material：Bacterium material strip prepared by step (4) is laid with, and timber section is placed in below soil, in timber section
Poria mycelium is grown naturally in the soil.
2. the cultural method of Poria cocos according to claim 1, which is characterized in that step（1）In, the sawdust is selected from horse hair
Pine, Pinus taiwanesis, one or more of Japanese red pine, black pine, larch, Chinese fir.
3. the cultural method of Poria cocos according to claim 1, which is characterized in that step（1）In, the multi-vitamins packet
Containing vitamin A, vitamine D3, vitamin E, vitamin B1 and vitamin B6, and every kilogram of multi-vitamins contain：Vitamin A
15000000IU, vitamine D3 6000000, vitamin E 100000IU, vitamin B1 12000mg, vitamin B6
16000mg and surplus are carrier defatted rice bran.
4. the cultural method of Poria cocos according to claim 1, which is characterized in that step（1）In, the trace element addition
Agent prescription is that every kilogram of trace mineral supplement contains：Copper 25mg, iron 400mg, zinc 500mg, manganese 500mg, iodine 15mg, selenium
1.5mg, cobalt 10mg and surplus are carrier zeolite.
5. the cultural method of Poria cocos according to claim 1, which is characterized in that step（2）In, the side of the sterilizing
Method：Cultivating bacteria bag is placed in 0.15MPa pressure, sterilize at 120 DEG C 2h, or with 100 DEG C of flowing steam steam sterilizing 5-7h.
6. the cultural method of Poria cocos according to claim 1, which is characterized in that the volume of the cultivating bacteria bag is Φ 15cm
× 35cm is 650g per packed culture doses.
7. the cultural method of Poria cocos according to claim 1, which is characterized in that step（3）In, when culture Poria cocos cultivates bacterium
Inoculum concentration be：It is inoculated with according to envelope-bulk to weight ratio of the Poria cocos mother bacterium 6ml with culture base-material 330-400g.
8. the cultural method of Poria cocos according to claim 1, which is characterized in that step（4）In, further include to timber section into
Sector-style does sterilization processing.
9. the cultural method of Poria cocos according to claim 8, which is characterized in that timber section is air-dried by nature solarization,
So that timber section water content is less than 15%, then timber section is put into wet sterilization pot, carries out steam sterilization.
10. the cultural method of Poria cocos according to claim 1, which is characterized in that step（6）In, it is covered in timber section
The thickness of soil of side is no more than 4cm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110651663A (en) * | 2019-11-12 | 2020-01-07 | 吴丽娟 | Production method of green selenium-rich poria cocos |
| CN114051887A (en) * | 2021-11-19 | 2022-02-18 | 云茯苓普洱有限公司 | Indoor planting method of poria cocos |
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| CN1732761A (en) * | 2005-07-29 | 2006-02-15 | 四川省农业科学院生物技术核技术研究所 | A kind of method of cultivating Poria cocos |
| CN104284578A (en) * | 2013-03-27 | 2015-01-14 | 株式会社津村 | Method for culturing wolfiporia cocos |
| CN104969775A (en) * | 2015-07-29 | 2015-10-14 | 句容美华园林景观发展有限公司 | Method for cultivating Indian bread |
| CN105036940A (en) * | 2015-07-29 | 2015-11-11 | 句容美华园林景观发展有限公司 | Poria cocos strain compost and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1732761A (en) * | 2005-07-29 | 2006-02-15 | 四川省农业科学院生物技术核技术研究所 | A kind of method of cultivating Poria cocos |
| CN104284578A (en) * | 2013-03-27 | 2015-01-14 | 株式会社津村 | Method for culturing wolfiporia cocos |
| CN104969775A (en) * | 2015-07-29 | 2015-10-14 | 句容美华园林景观发展有限公司 | Method for cultivating Indian bread |
| CN105036940A (en) * | 2015-07-29 | 2015-11-11 | 句容美华园林景观发展有限公司 | Poria cocos strain compost and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110651663A (en) * | 2019-11-12 | 2020-01-07 | 吴丽娟 | Production method of green selenium-rich poria cocos |
| CN114051887A (en) * | 2021-11-19 | 2022-02-18 | 云茯苓普洱有限公司 | Indoor planting method of poria cocos |
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