CN105130660A - High-efficiency culture medium for cultivating edible fluorescent oyster mushrooms and preparation method of high-efficiency culture medium - Google Patents

Abstract

The invention discloses a high-efficiency culture medium for cultivating edible fluorescent oyster mushrooms. The high-efficiency culture medium comprises the following components in part by weight: pumpkin vine, walnut shell, dry fermented poultry manure, onion skin, hawthorn powder, sophora japonica, sodium hydrogen phosphate, black bean meal, glutinous rice germ, cassava alcohol, compound enzyme, lolium perenne, histidine, leech powder, sunflower seed hull, calcium hydrogen phosphate, locust powder, animal pancreas powder, aspartame, basic zinc carbonate, perilla color, edible fluorescent silk protein, protease and carbon nano-tubes. According to the high-efficiency culture medium disclosed by the invention, composite powder such as expanded pumpkin vine is adopted to provide a loose breathable environment for the growth of the oyster mushrooms; the dry fermented poultry manure and the like are adopted to provide abundant nutrition for the cultivation of the oyster mushrooms; edible fluorescent silk protein hydrolysate, the perilla color and the basic zinc carbonate as an enzyme active substance are loaded in modified carbon nano-tubes so as to promote high-efficiency cultivation of the edible fluorescent oyster mushrooms. The oyster mushrooms cultivated by the culture medium disclosed by the invention are large in size and good in mouthfeel, have edible fluorescent color and luster, and are deeply favored by the public.

Description

A kind of efficient culture medium for cultivating edible fluorescence flat mushroom and preparation method thereof

Technical field

The present invention relates to culture medium of edible fungus technical field, particularly relating to a kind of efficient culture medium for cultivating edible fluorescence flat mushroom and preparation method thereof.

Background technology

The nutrition of edible mushrooms is high, and along with the raising of people's living standard, people require to increase gradually to the demand of edible mushrooms and demand, while improving the nutrition using bacterium, also need the kind enriching edible mushrooms.The environmental protection edible mushrooms of being enriched by cultivation technique exploitation nutrient efficient is the focus of research at present.

Zinc is as a kind of enzymic activity material, and its trophic function is relevant with multiple enzyme, and be the component of some desaturases, carbonic anhydrase and Phospholipid hydrolase, these enzymes play an important role to the Substance P hydrolysis in plant materials, redox processes and protein synthesis; Moreover zinc nutrition participates in the synthesis of growth hormone indolylacetic acid; Simultaneously zinc nutrition can the ribosomal neccessary composition of stabilized cell participate in chlorophyllous formation, but inorganic zinc easily runs off, and assimilated efficiency is low.Carbon nanotube as the carrier of enzymic activity material, can promote that enzymic activity material enters in edible mushrooms cell, promotes the growth of edible mushrooms; Meanwhile, along with, people are to the increase of organic colored food demand amount, and genetic modification can increase the kind of organic colored food, but, there is certain difficulty.Edible non-toxic pigment enters in cell by carbon nanotube, can participate in the synthesis Sum decomposition of cell directly, promote the cultivation of organic colored edible fungus variety.

Summary of the invention

The object of the invention is exactly the defect in order to make up prior art, provides a kind of efficient culture medium for cultivating edible fluorescence flat mushroom and preparation method thereof.

The present invention is achieved by the following technical solutions:

A kind of efficient culture medium for cultivating edible fluorescence flat mushroom, comprise following parts by weight of component: Cushaw stem 100-110, nut-shell 20-22, dry fermentation poultry manure 40-42, onion-skin 15-17, hawthorn powder 23-24, sophora flower 19-20, sodium hydrogen phosphate 9-10, black soya bean dregs of beans 14-15, glutinous rice plumule 19-19.4, cassava grain stillage 20-21, composite enzyme 8-9, rye grass 11-12, Histidine 4-5, leech powder 8-8.4, sunflower seed shell 11-12, secondary calcium phosphate 3-4, locust powder 4-5, animal pancreas powder 3.4-3.6, protein sugar 2.3-2.5, zinc subcarbonate 2.5-2.6, Perilla color 1.8-2, the edible fluorescin 1.5-1.7 of silk, proteolytic enzyme 2-3, carbon nanotube 4-5, appropriate deionized water, appropriate water.

For cultivating an efficient culture medium preparation method for edible fluorescence flat mushroom, comprise the following steps:

(1) the edible fluorescin of silk, proteolytic enzyme and gross weight 3-5 deionized water mixing is doubly got, at 28-30 DEG C, stir enzymolysis 2-3h obtain edible fluorescin enzymolysis liquid, edible fluorescin enzymolysis liquid, half carbon nanotube, Perilla color and gross weight 2-3 deionized water is doubly mixed, adopt clarifixator with 20000r/min homogeneous 30-32min, the modified carbon nano-tube that spraying dry must load edible pigment is for subsequent use; Mixed by the carbon nanotube of zinc subcarbonate, protein sugar, residue half, and the deionized water adding gross weight 2-3 times mixes, adopt clarifixator with 20000r/min homogeneous 25-27min, the modified carbon nano-tube that spraying dry must load zinc is for subsequent use;

(2) Cushaw stem, onion-skin, hawthorn powder, sophora flower, nut-shell, sunflower seed shell, rye grass mixing are put into decompressor and to be expanded 20-30min, put into pulverizer afterwards, pulverize 30-35min, obtain expansion composite powder for subsequent use; Fermentation poultry manure, black soya bean dregs of beans, glutinous rice plumule, cassava grain stillage, locust powder, the mixing of animal pancreas powder will be done, put into pulverizer afterwards and pulverize 30-40min, obtain high protein composite powder for subsequent use; By expansion composite powder, high protein composite powder mixing, and adjust moisture content moderate after, carry out compost 25-27d, turning 3-4 time in composting process, after compost terminates, spreading airing to moisture content out is the 20-22% of weight, sterilizing 2-3h at high temperature 90 DEG C, obtains compost for subsequent use simultaneously;

(3) leech powder is heated 2-3h at 180 DEG C, cool to obtain modification leech powder; By modification leech powder, load the modified carbon nano-tube of edible pigment, the modified carbon nano-tube mixing and stirring of year zinc, carbon mano-tube composite must be disperseed for subsequent use; By sodium hydrogen phosphate, composite enzyme, Histidine, secondary calcium phosphate and gross weight 4-5 water mixing doubly, stir, obtain nutritive medium for subsequent use;

(4) by compost, dispersing Nano carbon tubes mixture, nutritive medium mixing and stirring, and adjust the 20.2-21% that moisture content is gross weight, after packed in 90-100 DEG C of high-temperature steam high-temperature sterilization 20-30min and get final product.

Advantage of the present invention is:

The present invention adopts the composite powders such as expansion Cushaw stem to be that the growth of flat mushroom provides loose ventilative ventilative environment; Employing dry fermentation poultry manure etc. is that the cultivation of flat mushroom provides abundant nutrition; Adopt silk edible fluorescin enzymolysis liquid and Perilla color and enzymic activity material zinc subcarbonate to be loaded in modified carbon nano-tube, promote the Efficient Cultivation of edible fluorescence flat mushroom; Adopt leech powder, facilitating carbon nanotube adds loading distribution in the medium on the one hand, adds the water retention property of substratum on the other hand; The onion-skin containing wide anti-microbial effect is adopted to have the effect suppressing miscellaneous bacteria.The flat mushroom of substratum of the present invention cultivation have large, mouthfeel is good, with edible fluorescence color and luster, deeply like by masses.

Embodiment

A kind of efficient culture medium for cultivating edible fluorescence flat mushroom, comprise following parts by weight of component: Cushaw stem 100, nut-shell 20, dry fermentation poultry manure 40, onion-skin 15, hawthorn powder 23, sophora flower 19, sodium hydrogen phosphate 9, black soya bean dregs of beans 14, glutinous rice plumule 19, cassava grain stillage 20, composite enzyme 8, rye grass 11, Histidine 4, leech powder 8, sunflower seed shell 11, secondary calcium phosphate 3, locust powder 4, animal pancreas powder 3.4, protein sugar 2.3, zinc subcarbonate 2.5, Perilla color 1.8, the edible fluorescin 1.5 of silk, proteolytic enzyme 2, carbon nanotube 4, appropriate deionized water, appropriate water.

For cultivating an efficient culture medium preparation method for edible fluorescence flat mushroom, comprise the following steps:

(1) the deionized water mixing of the edible fluorescin of silk, proteolytic enzyme and gross weight 3 times is got, at 28 DEG C, stir enzymolysis 2h obtain edible fluorescin enzymolysis liquid, the deionized water of edible fluorescin enzymolysis liquid, half carbon nanotube, Perilla color and gross weight 2 times is mixed, adopt clarifixator with 20000r/min homogeneous 30min, the modified carbon nano-tube that spraying dry must load edible pigment is for subsequent use; Mixed by the carbon nanotube of zinc subcarbonate, protein sugar, residue half, and the deionized water adding gross weight 2 times mixes, adopt clarifixator with 20000r/min homogeneous 25min, the modified carbon nano-tube that spraying dry must load zinc is for subsequent use;

(2) Cushaw stem, onion-skin, hawthorn powder, sophora flower, nut-shell, sunflower seed shell, rye grass mixing are put into decompressor and to be expanded 20min, put into pulverizer afterwards, pulverize 30min, obtain expansion composite powder for subsequent use; Fermentation poultry manure, black soya bean dregs of beans, glutinous rice plumule, cassava grain stillage, locust powder, the mixing of animal pancreas powder will be done, put into pulverizer afterwards and pulverize 30min, obtain high protein composite powder for subsequent use; By expansion composite powder, high protein composite powder mixing, and adjust moisture content moderate after, carry out compost 25d, turning 3 times in composting process, after compost terminates, spreading airing to moisture content out is 20% of weight, sterilizing 2h at high temperature 90 DEG C, obtains compost for subsequent use simultaneously;

(3) leech powder is heated 2h at 180 DEG C, cool to obtain modification leech powder; By modification leech powder, load the modified carbon nano-tube of edible pigment, the modified carbon nano-tube mixing and stirring of year zinc, carbon mano-tube composite must be disperseed for subsequent use; The water of sodium hydrogen phosphate, composite enzyme, Histidine, secondary calcium phosphate and gross weight 4 times is mixed, stirs, obtain nutritive medium for subsequent use;

(4) by compost, dispersing Nano carbon tubes mixture, nutritive medium mixing and stirring, and adjust that moisture content is gross weight 20.2%, after packed in 90 DEG C of high-temperature steams high-temperature sterilization 20min and get final product.

Claims (2)

1. one kind for cultivating the efficient culture medium of edible fluorescence flat mushroom, it is characterized in that, comprise following parts by weight of component: Cushaw stem 100-110, nut-shell 20-22, dry fermentation poultry manure 40-42, onion-skin 15-17, hawthorn powder 23-24, sophora flower 19-20, sodium hydrogen phosphate 9-10, black soya bean dregs of beans 14-15, glutinous rice plumule 19-19.4, cassava grain stillage 20-21, composite enzyme 8-9, rye grass 11-12, Histidine 4-5, leech powder 8-8.4, sunflower seed shell 11-12, secondary calcium phosphate 3-4, locust powder 4-5, animal pancreas powder 3.4-3.6, protein sugar 2.3-2.5, zinc subcarbonate 2.5-2.6, Perilla color 1.8-2, the edible fluorescin 1.5-1.7 of silk, proteolytic enzyme 2-3, carbon nanotube 4-5, appropriate deionized water, appropriate water.

2., for cultivating an efficient culture medium preparation method for edible fluorescence flat mushroom, it is characterized in that, comprise the following steps:

(1) the edible fluorescin of silk, proteolytic enzyme and gross weight 3-5 deionized water mixing is doubly got, at 28-30 DEG C, stir enzymolysis 2-3h obtain edible fluorescin enzymolysis liquid, edible fluorescin enzymolysis liquid, half carbon nanotube, Perilla color and gross weight 2-3 deionized water is doubly mixed, adopt clarifixator with 20000r/min homogeneous 30-32min, the modified carbon nano-tube that spraying dry must load edible pigment is for subsequent use; Mixed by the carbon nanotube of zinc subcarbonate, protein sugar, residue half, and the deionized water adding gross weight 2-3 times mixes, adopt clarifixator with 20000r/min homogeneous 25-27min, the modified carbon nano-tube that spraying dry must load zinc is for subsequent use;

(2) Cushaw stem, onion-skin, hawthorn powder, sophora flower, nut-shell, sunflower seed shell, rye grass mixing are put into decompressor and to be expanded 20-30min, put into pulverizer afterwards, pulverize 30-35min, obtain expansion composite powder for subsequent use; Fermentation poultry manure, black soya bean dregs of beans, glutinous rice plumule, cassava grain stillage, locust powder, the mixing of animal pancreas powder will be done, put into pulverizer afterwards and pulverize 30-40min, obtain high protein composite powder for subsequent use; By expansion composite powder, high protein composite powder mixing, and adjust moisture content moderate after, carry out compost 25-27d, turning 3-4 time in composting process, after compost terminates, spreading airing to moisture content out is the 20-22% of weight, sterilizing 2-3h at high temperature 90 DEG C, obtains compost for subsequent use simultaneously;

(3) leech powder is heated 2-3h at 180 DEG C, cool to obtain modification leech powder; By modification leech powder, load the modified carbon nano-tube of edible pigment, the modified carbon nano-tube mixing and stirring of year zinc, carbon mano-tube composite must be disperseed for subsequent use; By sodium hydrogen phosphate, composite enzyme, Histidine, secondary calcium phosphate and gross weight 4-5 water mixing doubly, stir, obtain nutritive medium for subsequent use;

(4) by compost, dispersing Nano carbon tubes mixture, nutritive medium mixing and stirring, and adjust the 20.2-21% that moisture content is gross weight, after packed in 90-100 DEG C of high-temperature steam high-temperature sterilization 20-30min and get final product.