CN103911310A - Organic material decomposing agent and its preparation method - Google Patents

Organic material decomposing agent and its preparation method Download PDF

Info

Publication number
CN103911310A
CN103911310A CN201310004556.3A CN201310004556A CN103911310A CN 103911310 A CN103911310 A CN 103911310A CN 201310004556 A CN201310004556 A CN 201310004556A CN 103911310 A CN103911310 A CN 103911310A
Authority
CN
China
Prior art keywords
days
compost
temperature
thermophilic
aspergillus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310004556.3A
Other languages
Chinese (zh)
Inventor
汪丽刚
程玉海
王慧芳
王建红
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HEBI CITY RENYUAN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
Original Assignee
HEBI CITY RENYUAN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HEBI CITY RENYUAN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd filed Critical HEBI CITY RENYUAN BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
Priority to CN201310004556.3A priority Critical patent/CN103911310A/en
Publication of CN103911310A publication Critical patent/CN103911310A/en
Pending legal-status Critical Current

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention belongs to the technical field of environmental microorganisms, and especially relates to an organic material decomposing agent and its preparation method. The preparation method comprises the following steps: preparing strains, drying, crushing, sieving, and mixing 35-55 parts of Bacillus spp with 2-5 parts of yeast, 5-10 parts of aspergillus, 4-20 parts of Streptomyces thermophilus, 8-15 parts of Thermoactinomyces sp and 20-30 parts of high temperature cellulose decomposition bacteria. The organic material decomposing agent can utilize easy-degradation components comprising starch, proteins and the like in a compost in order to accelerate the heating of the compost, and can also secrete digestive enzyme to accelerate the decomposition of difficult-decomposition substances comprising pectin, cellulose, lignin and the like without interfering the full performance of the microorganisms, so the composting efficiency and the quality of a composted finished product are improved, and the organic material decomposing agent has the advantages of low price, wide application range, strong practicability and easy popularization.

Description

Organic matter decomposing inoculant and preparation method thereof
Technical field
The invention belongs to environmental microorganism technical field, relate in particular to a kind of organic matter decomposing inoculant and preparation method thereof.
Background technology
Along with the fast development of Animal Husbandry in China, feces of livestock and poultry has become one of important source of pollution of contaminate environment, and its resource utilization, Reduction Technology do not catch up with production rate simultaneously; Crop straw burning problem; not only welding; wasted valuable agricultural resource simultaneously; the resource utilizations such as industry organic waste residues (grouts, chaff shell etc.), mud and city organic waste, processing of farm products dead meal (sugarcane bagasse, pomace, mushroom residue, vinasse, furfural dregs) and innoxious process for treating are not mature enough; become serious source of pollution; solve the outlet of these organic waste, protection of the environment, is current urgent problem.Aerobic compost is the important means of agricultural waste resource utilization and harmless treatment, is also one of effective way promoting sustainable development agricultural.Aerobic compost technology is that organic solid waste is mixed according to a certain percentage with stopping composition, under the condition of suitable moisture, pH, oxygen, carbon-nitrogen ratio, make microbial reproduction and the organic matter of degrading, and produce high temperature, kill pathogenic bacteria and weed seed wherein, make organism reach the process of stabilization.Traditional aerobic compost all utilizes the indigenous microorganism in windrow to carry out metabolism, decomposing organic matter, owing to differing in material source, randomness and the randomness with indigenous microorganism kind, also because the cell walls of plant tissue in agricultural wastes is mainly by pectin, Mierocrystalline cellulose, hemicellulose and xylogen be chimeric forming mutually, Stability Analysis of Structures and be difficult for degraded, especially wherein the difficult degradation character of lignocellulose has hindered effective degraded of indigenous microorganism to agricultural wastes, this has just caused traditional compost poor controllability, produce stink, fermentation required time is partially long, the problem that compost finished product fertilizer efficiency is too low.
In addition, can the speed of compost be to be related to the important factor that improve compost benefit.Compost material comprises the solid waste such as mud, rubbish, crop material, feces of livestock and poultry, and its composition all has monose, protein, lipid, Mierocrystalline cellulose and hemicellulose and xylogen etc. to form in varing proportions, wherein the shared proportion maximum of Mierocrystalline cellulose.Thereby in compost maturity process, cellulose decomposition is crucial: 1, Mierocrystalline cellulose quantity maximum, accounts for 40 ~ 60% of plant dry matter, and decompose slow, nonnitrogenous organic substance.2, cellulosic destruction means the disintegration of cellular material and the generation of incorporeity material, and this is the most important physical form variation in compost maturity process.Therefore study cellulolytic microorganism significant for solving environmental pollution and energy problem.Compost temperature is the mark of microbial activities situation, piles the speed of warm height decision compost speed.In During High-Temperature Composting process, accelerating heat-up rate, shortening the compost maturity time is the key that organic commercial fertilizer produces economic benefit.
At present, for improving composting process, the research that improves compost final product quality mainly concentrates on developing of all kinds of microbial inoculums and the application in composting system thereof.Designing more is the single dominant bacterial classification that by access, Cucumber in windrow is had special degradation capability, or the composite fungus agent of the multiple microorganism combination with degraded different substances ability is accelerated composting process.As the land of happiness bacterium of Japanese BOM bacterium, EM bacterium, Korea S etc. also has application in China, but price is higher, function singleness, and liquid state is used inconvenience, and popularization is restricted; As disclosed a kind of fast microbiological fermenting agent of chicken manure in patent documentation " fast microbiological fermenting agent and the fermentation process of chicken manure " (C N 200410072694.6), mixed by viride, actinomycetes, photosynthetic bacterium, thermophilic microorganism and high temperature genus bacillus, yeast, Bacillus licheniformis; Document patent " utilizing microorganism quick fermentation pig manure to produce the method for organic commercial fertilizer " (CN200710079090.8) has proposed a kind of fermenting agent being made up of genus bacillus, lactobacillus, photosynthetic bacterium, vinelandii, yeast, filamentous fungus etc.; Patent documentation " for fermenting agent of agricultural wastes processing and preparation method thereof " (200410039818.0) discloses saccharomycopsis fibuligera bacterium ZJY2 and two kinds of new bacterial strains of grey gamboge streptomycete W1 and another known bacterial classification bacillus cereus ME1-2.But at present existing these artificial interpolation microbial inoculums or specificity are excessively strong, only the waste of single kind are had to positive effect; Or shortage specific aim, different composting materials uses same preparation, is inoculated in after different composting materials, and the microflora's adaptive faculty in fermenting agent is poor, a large amount of useful Microbial resources of loss, reduction fermentation efficiency; Or adopt bacterial classification too many, and in microbial inoculum proportioning, do not form dominant bacteria, and produce difficulty, affect its effect.At present, most of research reports too bias toward the impact of microbial inoculum on laboratory compost result, or because bacterial classification quantity causes microbial inoculum practicality not strong less, or because microbial inoculum from abroad, price is high, causes the popularization of microbial inoculum to be obstructed.Therefore be necessary that research has the cheap ight soil high efficiency degradation bacterial agent of independent intellectual property right.
Summary of the invention
A kind of easily composition of degradation product such as starch based, protein that can utilize in windrow that the object of the invention is to overcome the deficiencies in the prior art and provide, heats up to accelerate windrow; Again can be on the basis of not disturbing indigenous microorganism to play one's part to the full, secretion degrading enzyme accelerates the decomposition of the hard-degraded substances such as pectin, Mierocrystalline cellulose, xylogen, so improve composting efficiency and compost final product quality and cheap, applied widely, practical, be convenient to organic matter decomposing inoculant of promoting and preparation method thereof.
The object of the present invention is achieved like this:
A kind of organic matter decomposing inoculant, it is to be made up according to following weight proportion of each component: genus bacillus 35-55 part, yeast 2-5 part, aspergillus 5-10 part, thermophilic mould 4-20 part, thermophilic actinomycete 8-15 part, high temperature fiber element decomposing bacteria 20-30 part.
Described genus bacillus is one or several in subtilis, Bacillus licheniformis, subtilis, bacillus megaterium, bacillus cereus.
Described yeast is the one in Candida utilis bacterium, Endomycopsis Fibnligera, saccharomyces cerevisiae.
Described aspergillus is the one in aspergillus niger, aspergillus oryzae.
Described thermophilic actinomycete is the one in thermostreptomyces.
Described thermophilic mould is the one in chaetomium thermophilum, sporotrichum thermophile.
The preparation method of described organic matter decomposing inoculant, this preparation method comprises the steps:
The preparation of step 1, bacterial classification:
1) cultivation of genus bacillus: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 30-40 DEG C, cultivate 3 days, after testing, be greater than 1,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
2) cultivation of high temperature fiber element decomposing bacteria: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 50-60 DEG C, cultivate 2 days, after testing, be greater than 3,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
3) cultivation of thermophilic actinomycete: thermophilic actinomycete is seeded on solid multiplication substratum with the inoculum size of 5-15%, the temperature that controls environment 50-65 DEG C, cultivate 3-5 days, after testing, be greater than 1,000,000,000/g to thermophilic actinomycete content, described solid multiplication substratum is to be made up according to following weight percent of each component: sawdust 30%, wheat bran 54%, Semen Maydis powder 5%, dregs of beans 10%, magnesium sulfate 0.8%, sodium hydroxide 0.2%;
4) cultivation of thermophilic mould: thermophilic mould is seeded in to water ratio taking the form of liquid seeds in the solid-state fermentation culture medium of 50-60% according to the inoculum size of 2-10%, at 45-55 DEG C, cultivate 3 days, after testing, extremely thermophilic mould bacterial content is greater than 1,000,000,000/g, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 40%, wheat bran 40%, dregs of beans 20%;
5) cultivation of aspergillus: aspergillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 25-35 DEG C, cultivate 4-7 days, after testing, be greater than 500,000,000/g to aspergillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
6) saccharomycetic cultivation: yeast is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 28-35 DEG C, cultivate 3-7 days, after testing, be greater than 200,000,000/g to the mould content of yeast, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
Step 2, drying treatment: genus bacillus that step 1 is obtained, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material be in the seasoning of airing field room temperature, be down to below 10% to water ratio;
Step 3, pulverize and sieve: dry step 2 genus bacillus obtaining, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material were pulverized to 60 mesh sieves, and extracting screen underflow, is former bacterium powder;
Step 4, mix: step 3 is pulverized and sieved to the genus bacillus that obtains, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic former bacterium powder and mix and make organic matter decomposing inoculant according to following weight proportion: genus bacillus 35-55 part, yeast 2-5 part, aspergillus 5-10 part, thermophilic mould 4-20 part, thermophilic actinomycete 8-15 part, high temperature fiber element decomposing bacteria 20-30 part.
Compared to the prior art, the present invention has the following advantages:
The present invention utilizes normal fermentation mode, sets up that normal temperature, middle temperature, high temperature are collaborative to go forward one by one, have complementary functions, and enzyme is complementary high efficiency flora system, gives full play to the advantage of each flora, and use range is wider, suitability is stronger, and degradation efficiency is higher.In the present invention, genus bacillus bacterium is the Main Function flora of middle thermophase, fermentation calefaction is played a leading role, yeast helps out to heating up, although aspergillus just plays a role to organic decomposition in compost temperature-rise period, but by machine thing is decomposed, releasing energy can be other microorganisms necessary nutrient is provided, and then is conducive to the intensification of compost, finally completes compost maturity process at temperature raising period and other microbial population actings in conjunction.Thermophilic actinomycete, high temperature cellulose-decomposting bacteria and thermophilic mould are high temperature section main species, extremely strong 50 DEG C of left and right decomposition of cellulose activity, if the speed of sporotrichum thermophile degraded cellulose is 5 times of wooden mould normal temperature bacterial strain.They decompose strongly to organism (as Mierocrystalline cellulose, hemicellulose, pectin substance etc.) complicated in compost, have accelerated compost process, have shortened the time of becoming thoroughly decomposed.
The present invention, by introducing specific microorganism, becomes the dominant microflora in material after adding material, to accelerate the maturity of compost, coordinates the degradation process of different degradation capability materials, thereby effectively shortens material decomposed period and the guarantee quality of becoming thoroughly decomposed.The present invention can increase in use microbial count in heap layer, regulates compost flora structure, improve microorganism active, due to the mutual synergy between each bacterial classification, form complexity and the stable ecosystem, microorganism in heap layer is bred rapidly, heap temperature rise to 65 DEG C hot stage, mainly contain thermophilic actinomycete, high temperature fiber decomposer, the strong decompose pectin of high temperature fiber decomposer, fine micro-element, hemicellulose and part xylogen, and carry out humification process.Then cool to below 50 DEG C, now in heap, microbe species and quantity are all many than previous stage, and middle warm nature microbe population significantly increases, and humification is preponderated.The present invention can utilize the easily composition of degradation product such as starch based, protein in windrow, heats up to accelerate windrow; Again can be on the basis of not disturbing indigenous microorganism to play one's part to the full, secretion degrading enzyme accelerates the decomposition of the hard-degraded substances such as pectin, Mierocrystalline cellulose, xylogen, and then improves composting efficiency and compost final product quality; In addition, bacterial classification used of the present invention is the domestic conventional bacterial classification that can buy, low price, easily realizes and promotes.
Embodiment
Below in conjunction with embodiment, the present invention is described further.
Embodiment 1
A kind of organic matter decomposing inoculant, it is formulated according to following weight by each component: genus bacillus 35g, yeast 2g, aspergillus 5g, thermophilic mould 4g, thermophilic actinomycete 8g, high temperature fiber element decomposing bacteria 20g.
Described genus bacillus is one or several in subtilis, Bacillus licheniformis, subtilis, bacillus megaterium, bacillus cereus.
Described yeast is the one in Candida utilis bacterium, Endomycopsis Fibnligera, saccharomyces cerevisiae.
Described aspergillus is the one in aspergillus niger, aspergillus oryzae.
Described thermophilic actinomycete is the one in thermostreptomyces.
Described thermophilic mould is the one in chaetomium thermophilum, sporotrichum thermophile.
The preparation method of described organic matter decomposing inoculant, this preparation method comprises the steps:
The preparation of step 1, bacterial classification:
1) cultivation of genus bacillus: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 30-40 DEG C, cultivate 3 days, after testing, be greater than 1,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
2) cultivation of high temperature fiber element decomposing bacteria: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 50-60 DEG C, cultivate 2 days, after testing, be greater than 3,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
3) cultivation of thermophilic actinomycete: thermophilic actinomycete is seeded on solid multiplication substratum with the inoculum size of 5-15%, the temperature that controls environment 50-65 DEG C, cultivate 3-5 days, after testing, be greater than 1,000,000,000/g to thermophilic actinomycete content, described solid multiplication substratum is to be made up according to following weight percent of each component: sawdust 30%, wheat bran 54%, Semen Maydis powder 5%, dregs of beans 10%, magnesium sulfate 0.8%, sodium hydroxide 0.2%;
4) cultivation of thermophilic mould: thermophilic mould is seeded in to water ratio taking the form of liquid seeds in the solid-state fermentation culture medium of 50-60% according to the inoculum size of 2-10%, at 45-55 DEG C, cultivate 3 days, after testing, extremely thermophilic mould bacterial content is greater than 1,000,000,000/g, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 40%, wheat bran 40%, dregs of beans 20%;
5) cultivation of aspergillus: aspergillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 25-35 DEG C, cultivate 4-7 days, after testing, be greater than 500,000,000/g to aspergillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
6) saccharomycetic cultivation: yeast is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 28-35 DEG C, cultivate 3-7 days, after testing, be greater than 200,000,000/g to the mould content of yeast, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
Step 2, drying treatment: genus bacillus that step 1 is obtained, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material be in the seasoning of airing field room temperature, be down to below 10% to water ratio;
Step 3, pulverize and sieve: dry step 2 genus bacillus obtaining, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material were pulverized to 60 mesh sieves, and extracting screen underflow, is former bacterium powder;
Step 4, mix: step 3 is pulverized and sieved to the genus bacillus that obtains, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic former bacterium powder and mix and make organic matter decomposing inoculant according to following weight: genus bacillus 35g, yeast 2g, aspergillus 5g, thermophilic mould 4g, thermophilic actinomycete 8g, high temperature fiber element decomposing bacteria 20g.
The use of organism decomposing agent of the present invention:
1) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse cow dung per ton the consumption that weight percent is 0.1% Solid Organic refuse cow dung processed, and with do not throw in microbial inoculum Solid Organic refuse cow dung processing naturally in contrast.First cow dung and mushroom residue are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, carbon-nitrogen ratio 30, pH=7.2, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 1;
Table 1 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (15 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention 52 64 68 68 69 67 67
Blank 15 23 42 47 53 52 49
Add as can be seen from Table 1 the processing heap leavening temperature of decomposing agent of the present invention and pile apparently higher than blank, under certain condition, leavening temperature remains at more than 60 DEG C, has reached the requirement of high temperature high-rate composting fermentation.China's decontamination of human excreta hygienic standard (GB7959-1987) regulation, the highest compost temperature reaches 50-55 DEG C and lasting 5-7 days, and compost reaches innoxious standard.And blank microbial activities is not obvious, leavening temperature changes with envrionment temperature substantially, heats up slowly and can not reach a high temperature, and is difficult to make cattle manure slaking.In compost, find, prior fermentation temperature is rapid ascendant trend, then be generally slow decreasing trend, show under microbial inoculum effect, in cow dung, most labile organism can be decomposed at short notice, by turning, within 20 days subsequently left and right time, present Secondary Fermentation feature, still undecomposed organism further decomposes.This fermentation maturity phenomenon and rule, basically identical with the cow dung During High-Temperature Composting fermentative processing of adding external similar microbial inoculum.
Meanwhile, the smells such as the normal foul smelling of cow dung, mainly comprise that voltaile fatty acid, alcohols, phenols, aldehydes, thio-alcohol etc. more than 90 plant material.Deodorizing is one of requirement of compost treatment, in the present embodiment, cow dung after decomposing microbial inoculum compost treatment, odorless substantially.From change of soil water content, processing heap is because heap temperature is high, and moisture evaporation is fast, will be obvious under water content, and contrast heap temperature is low, moisture volatilizees naturally slowly.C/N and pH are the whether basic indexs of slaking of reflection compost maturing process and compost, when the C/N in compost drops to below 20 and when pH is down to subacidity from initial acid-basicity, compost is fermentation maturity.Very obvious with the decline of microbial inoculum processing, compost maturity speed is fast, and in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 30d.In addition, adopt radish seed, in the compost extraction liquid of 5% interpolation decomposing agent of the present invention, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 87%, and then definite compost maturity.Further, the compost color that decomposing agent processing of the present invention ferments is chocolate; Material form is profile and collapses, evenly tiny; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste; Water ratio, is drying regime, and hand does not become piece.
2) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 2;
Table 2 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 13 57 65 67 68 68
Blank -8 -6 0 9 23 42 42
The processing heap leavening temperature that adds as can be seen from Table 2 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 92%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.
3) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 3;
Table 3 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 13 57 65 67 68 68
Blank -8 -6 0 9 23 42 42
The processing heap leavening temperature that adds as can be seen from Table 3 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 92%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.
Embodiment 2
A kind of organic matter decomposing inoculant, it is formulated according to following weight by each component: genus bacillus 40g, yeast 3g, aspergillus 7g, thermophilic mould 10g, thermophilic actinomycete 10g, high temperature fiber element decomposing bacteria 23g.
Described genus bacillus is one or several in subtilis, Bacillus licheniformis, subtilis, bacillus megaterium, bacillus cereus.
Described yeast is the one in Candida utilis bacterium, Endomycopsis Fibnligera, saccharomyces cerevisiae.
Described aspergillus is the one in aspergillus niger, aspergillus oryzae.
Described thermophilic actinomycete is the one in thermostreptomyces.
Described thermophilic mould is the one in chaetomium thermophilum, sporotrichum thermophile.
The preparation method of described organic matter decomposing inoculant, this preparation method comprises the steps:
The preparation of step 1, bacterial classification:
1) cultivation of genus bacillus: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 30-40 DEG C, cultivate 3 days, after testing, be greater than 1,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
2) cultivation of high temperature fiber element decomposing bacteria: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 50-60 DEG C, cultivate 2 days, after testing, be greater than 3,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
3) cultivation of thermophilic actinomycete: thermophilic actinomycete is seeded on solid multiplication substratum with the inoculum size of 5-15%, the temperature that controls environment 50-65 DEG C, cultivate 3-5 days, after testing, be greater than 1,000,000,000/g to thermophilic actinomycete content, described solid multiplication substratum is to be made up according to following weight percent of each component: sawdust 30%, wheat bran 54%, Semen Maydis powder 5%, dregs of beans 10%, magnesium sulfate 0.8%, sodium hydroxide 0.2%;
4) cultivation of thermophilic mould: thermophilic mould is seeded in to water ratio taking the form of liquid seeds in the solid-state fermentation culture medium of 50-60% according to the inoculum size of 2-10%, at 45-55 DEG C, cultivate 3 days, after testing, extremely thermophilic mould bacterial content is greater than 1,000,000,000/g, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 40%, wheat bran 40%, dregs of beans 20%;
5) cultivation of aspergillus: aspergillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 25-35 DEG C, cultivate 4-7 days, after testing, be greater than 500,000,000/g to aspergillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
6) saccharomycetic cultivation: yeast is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 28-35 DEG C, cultivate 3-7 days, after testing, be greater than 200,000,000/g to the mould content of yeast, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
Step 2, drying treatment: genus bacillus that step 1 is obtained, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material be in the seasoning of airing field room temperature, be down to below 10% to water ratio;
Step 3, pulverize and sieve: dry step 2 genus bacillus obtaining, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material were pulverized to 60 mesh sieves, and extracting screen underflow, is former bacterium powder;
Step 4, mix: step 3 is pulverized and sieved to the genus bacillus that obtains, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic former bacterium powder and mix and make organic matter decomposing inoculant according to following weight: genus bacillus 40g, yeast 3g, aspergillus 7g, thermophilic mould 10g, thermophilic actinomycete 10g, high temperature fiber element decomposing bacteria 23g.
The use of organism decomposing agent of the present invention:
1) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse cow dung per ton the consumption that weight percent is 0.1% Solid Organic refuse cow dung processed, and with do not throw in microbial inoculum Solid Organic refuse cow dung processing naturally in contrast.First cow dung and mushroom residue are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, carbon-nitrogen ratio 30, pH=7.2, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 4;
Table 4 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (15 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention 53 65 69 69 70 68 68
Blank 14 24 41 48 52 50 47
Add as can be seen from Table 4 the processing heap leavening temperature of decomposing agent of the present invention and pile apparently higher than blank, under certain condition, leavening temperature remains at more than 60 DEG C, has reached the requirement of high temperature high-rate composting fermentation.China's decontamination of human excreta hygienic standard (GB7959-1987) regulation, the highest compost temperature reaches 50-55 DEG C and lasting 5-7 days, and compost reaches innoxious standard.And blank microbial activities is not obvious, leavening temperature changes with envrionment temperature substantially, heats up slowly and can not reach a high temperature, and is difficult to make cattle manure slaking.In compost, find, prior fermentation temperature is rapid ascendant trend, then be generally slow decreasing trend, show under microbial inoculum effect, in cow dung, most labile organism can be decomposed at short notice, by turning, within 20 days subsequently left and right time, present Secondary Fermentation feature, still undecomposed organism further decomposes.This fermentation maturity phenomenon and rule, basically identical with the cow dung During High-Temperature Composting fermentative processing of adding external similar microbial inoculum.
Meanwhile, the smells such as the normal foul smelling of cow dung, mainly comprise that voltaile fatty acid, alcohols, phenols, aldehydes, thio-alcohol etc. more than 90 plant material.Deodorizing is one of requirement of compost treatment, in the present embodiment, cow dung after decomposing microbial inoculum compost treatment, odorless substantially.From change of soil water content, processing heap is because heap temperature is high, and moisture evaporation is fast, will be obvious under water content, and contrast heap temperature is low, moisture volatilizees naturally slowly.C/N and pH are the whether basic indexs of slaking of reflection compost maturing process and compost, when the C/N in compost drops to below 20 and when pH is down to subacidity from initial acid-basicity, compost is fermentation maturity.Very obvious with the decline of microbial inoculum processing, compost maturity speed is fast, and in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 30d.In addition, adopt radish seed, in the compost extraction liquid of 5% interpolation decomposing agent of the present invention, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 88%, and then definite compost maturity.Further, the compost color that decomposing agent processing of the present invention ferments is chocolate; Material form is profile and collapses, evenly tiny; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste; Water ratio, is drying regime, and hand does not become piece.
2) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 5;
Table 5 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 14 58 66 69 70 70
Blank -8 -5 1 8 22 43 43
The processing heap leavening temperature that adds as can be seen from Table 5 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 93%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.
3) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 6;
Table 6 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 15 58 67 69 68 68
Blank -8 -6 1 8 23 41 41
The processing heap leavening temperature that adds as can be seen from Table 6 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 93%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.
Embodiment 3
A kind of organic matter decomposing inoculant, it is formulated according to following weight by each component: genus bacillus 50g, yeast 4g, aspergillus 9g, thermophilic mould 15g, thermophilic actinomycete 12g, high temperature fiber element decomposing bacteria 26g.
Described genus bacillus is one or several in subtilis, Bacillus licheniformis, subtilis, bacillus megaterium, bacillus cereus.
Described yeast is the one in Candida utilis bacterium, Endomycopsis Fibnligera, saccharomyces cerevisiae.
Described aspergillus is the one in aspergillus niger, aspergillus oryzae.
Described thermophilic actinomycete is the one in thermostreptomyces.
Described thermophilic mould is the one in chaetomium thermophilum, sporotrichum thermophile.
The preparation method of described organic matter decomposing inoculant, this preparation method comprises the steps:
The preparation of step 1, bacterial classification:
1) cultivation of genus bacillus: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 30-40 DEG C, cultivate 3 days, after testing, be greater than 1,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
2) cultivation of high temperature fiber element decomposing bacteria: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 50-60 DEG C, cultivate 2 days, after testing, be greater than 3,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
3) cultivation of thermophilic actinomycete: thermophilic actinomycete is seeded on solid multiplication substratum with the inoculum size of 5-15%, the temperature that controls environment 50-65 DEG C, cultivate 3-5 days, after testing, be greater than 1,000,000,000/g to thermophilic actinomycete content, described solid multiplication substratum is to be made up according to following weight percent of each component: sawdust 30%, wheat bran 54%, Semen Maydis powder 5%, dregs of beans 10%, magnesium sulfate 0.8%, sodium hydroxide 0.2%;
4) cultivation of thermophilic mould: thermophilic mould is seeded in to water ratio taking the form of liquid seeds in the solid-state fermentation culture medium of 50-60% according to the inoculum size of 2-10%, at 45-55 DEG C, cultivate 3 days, after testing, extremely thermophilic mould bacterial content is greater than 1,000,000,000/g, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 40%, wheat bran 40%, dregs of beans 20%;
5) cultivation of aspergillus: aspergillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 25-35 DEG C, cultivate 4-7 days, after testing, be greater than 500,000,000/g to aspergillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
6) saccharomycetic cultivation: yeast is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 28-35 DEG C, cultivate 3-7 days, after testing, be greater than 200,000,000/g to the mould content of yeast, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
Step 2, drying treatment: genus bacillus that step 1 is obtained, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material be in the seasoning of airing field room temperature, be down to below 10% to water ratio;
Step 3, pulverize and sieve: dry step 2 genus bacillus obtaining, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material were pulverized to 60 mesh sieves, and extracting screen underflow, is former bacterium powder;
Step 4, mix: step 3 is pulverized and sieved to the genus bacillus that obtains, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic former bacterium powder and mix and make organic matter decomposing inoculant according to following weight: genus bacillus 50g, yeast 4g, aspergillus 9g, thermophilic mould 15g, thermophilic actinomycete 12g, high temperature fiber element decomposing bacteria 26g.
The use of organism decomposing agent of the present invention:
1) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse cow dung per ton the consumption that weight percent is 0.1% Solid Organic refuse cow dung processed, and with do not throw in microbial inoculum Solid Organic refuse cow dung processing naturally in contrast.First cow dung and mushroom residue are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, carbon-nitrogen ratio 30, pH=7.2, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 7;
Table 7 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (15 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention 54 66 68 68 69 68 68
Blank 13 25 42 48 53 51 48
Add as can be seen from Table 7 the processing heap leavening temperature of decomposing agent of the present invention and pile apparently higher than blank, under certain condition, leavening temperature remains at more than 60 DEG C, has reached the requirement of high temperature high-rate composting fermentation.China's decontamination of human excreta hygienic standard (GB7959-1987) regulation, the highest compost temperature reaches 50-55 DEG C and lasting 5-7 days, and compost reaches innoxious standard.And blank microbial activities is not obvious, leavening temperature changes with envrionment temperature substantially, heats up slowly and can not reach a high temperature, and is difficult to make cattle manure slaking.In compost, find, prior fermentation temperature is rapid ascendant trend, then be generally slow decreasing trend, show under microbial inoculum effect, in cow dung, most labile organism can be decomposed at short notice, by turning, within 20 days subsequently left and right time, present Secondary Fermentation feature, still undecomposed organism further decomposes.This fermentation maturity phenomenon and rule, basically identical with the cow dung During High-Temperature Composting fermentative processing of adding external similar microbial inoculum.
Meanwhile, the smells such as the normal foul smelling of cow dung, mainly comprise that voltaile fatty acid, alcohols, phenols, aldehydes, thio-alcohol etc. more than 90 plant material.Deodorizing is one of requirement of compost treatment, in the present embodiment, cow dung after decomposing microbial inoculum compost treatment, odorless substantially.From change of soil water content, processing heap is because heap temperature is high, and moisture evaporation is fast, will be obvious under water content, and contrast heap temperature is low, moisture volatilizees naturally slowly.C/N and pH are the whether basic indexs of slaking of reflection compost maturing process and compost, when the C/N in compost drops to below 20 and when pH is down to subacidity from initial acid-basicity, compost is fermentation maturity.Very obvious with the decline of microbial inoculum processing, compost maturity speed is fast, and in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 30d.In addition, adopt radish seed, in the compost extraction liquid of 5% interpolation decomposing agent of the present invention, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 88%, and then definite compost maturity.Further, the compost color that decomposing agent processing of the present invention ferments is chocolate; Material form is profile and collapses, evenly tiny; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste; Water ratio, is drying regime, and hand does not become piece.
2) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 8;
Table 8 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 15 57 67 70 71 71
Blank -8 -6 2 9 23 44 44
The processing heap leavening temperature that adds as can be seen from Table 8 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 93%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.
3) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 9;
Table 9 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 16 59 68 70 69 69
Blank -8 -5 0 9 22 40 40
The processing heap leavening temperature that adds as can be seen from Table 9 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 94%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.
Embodiment 4
A kind of organic matter decomposing inoculant, it is formulated according to following weight by each component: genus bacillus 55g, yeast 5g, aspergillus 10g, thermophilic mould 20g, thermophilic actinomycete 15g, high temperature fiber element decomposing bacteria 30g.
Described genus bacillus is one or several in subtilis, Bacillus licheniformis, subtilis, bacillus megaterium, bacillus cereus.
Described yeast is the one in Candida utilis bacterium, Endomycopsis Fibnligera, saccharomyces cerevisiae.
Described aspergillus is the one in aspergillus niger, aspergillus oryzae.
Described thermophilic actinomycete is the one in thermostreptomyces.
Described thermophilic mould is the one in chaetomium thermophilum, sporotrichum thermophile.
The preparation method of described organic matter decomposing inoculant, this preparation method comprises the steps:
The preparation of step 1, bacterial classification:
1) cultivation of genus bacillus: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 30-40 DEG C, cultivate 3 days, after testing, be greater than 1,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
2) cultivation of high temperature fiber element decomposing bacteria: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 50-60 DEG C, cultivate 2 days, after testing, be greater than 3,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
3) cultivation of thermophilic actinomycete: thermophilic actinomycete is seeded on solid multiplication substratum with the inoculum size of 5-15%, the temperature that controls environment 50-65 DEG C, cultivate 3-5 days, after testing, be greater than 1,000,000,000/g to thermophilic actinomycete content, described solid multiplication substratum is to be made up according to following weight percent of each component: sawdust 30%, wheat bran 54%, Semen Maydis powder 5%, dregs of beans 10%, magnesium sulfate 0.8%, sodium hydroxide 0.2%;
4) cultivation of thermophilic mould: thermophilic mould is seeded in to water ratio taking the form of liquid seeds in the solid-state fermentation culture medium of 50-60% according to the inoculum size of 2-10%, at 45-55 DEG C, cultivate 3 days, after testing, extremely thermophilic mould bacterial content is greater than 1,000,000,000/g, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 40%, wheat bran 40%, dregs of beans 20%;
5) cultivation of aspergillus: aspergillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 25-35 DEG C, cultivate 4-7 days, after testing, be greater than 500,000,000/g to aspergillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
6) saccharomycetic cultivation: yeast is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 28-35 DEG C, cultivate 3-7 days, after testing, be greater than 200,000,000/g to the mould content of yeast, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
Step 2, drying treatment: genus bacillus that step 1 is obtained, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material be in the seasoning of airing field room temperature, be down to below 10% to water ratio;
Step 3, pulverize and sieve: dry step 2 genus bacillus obtaining, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material were pulverized to 60 mesh sieves, and extracting screen underflow, is former bacterium powder;
Step 4, mix: step 3 is pulverized and sieved to the genus bacillus that obtains, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic former bacterium powder and mix and make organic matter decomposing inoculant according to following weight: genus bacillus 55g, yeast 5g, aspergillus 10g, thermophilic mould 20g, thermophilic actinomycete 15g, high temperature fiber element decomposing bacteria 30g.
The use of organism decomposing agent of the present invention:
1) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse cow dung per ton the consumption that weight percent is 0.1% Solid Organic refuse cow dung processed, and with do not throw in microbial inoculum Solid Organic refuse cow dung processing naturally in contrast.First cow dung and mushroom residue are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, carbon-nitrogen ratio 30, pH=7.2, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 10;
Table 10 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (15 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention 11 66 69 69 70 68 68
Blank 12 24 43 49 54 52 49
Add as can be seen from Table 10 the processing heap leavening temperature of decomposing agent of the present invention and pile apparently higher than blank, under certain condition, leavening temperature remains at more than 60 DEG C, has reached the requirement of high temperature high-rate composting fermentation.China's decontamination of human excreta hygienic standard (GB7959-1987) regulation, the highest compost temperature reaches 50-55 DEG C and lasting 5-7 days, and compost reaches innoxious standard.And blank microbial activities is not obvious, leavening temperature changes with envrionment temperature substantially, heats up slowly and can not reach a high temperature, and is difficult to make cattle manure slaking.In compost, find, prior fermentation temperature is rapid ascendant trend, then be generally slow decreasing trend, show under microbial inoculum effect, in cow dung, most labile organism can be decomposed at short notice, by turning, within 20 days subsequently left and right time, present Secondary Fermentation feature, still undecomposed organism further decomposes.This fermentation maturity phenomenon and rule, basically identical with the cow dung During High-Temperature Composting fermentative processing of adding external similar microbial inoculum.
Meanwhile, the smells such as the normal foul smelling of cow dung, mainly comprise that voltaile fatty acid, alcohols, phenols, aldehydes, thio-alcohol etc. more than 90 plant material.Deodorizing is one of requirement of compost treatment, in the present embodiment, cow dung after decomposing microbial inoculum compost treatment, odorless substantially.From change of soil water content, processing heap is because heap temperature is high, and moisture evaporation is fast, will be obvious under water content, and contrast heap temperature is low, moisture volatilizees naturally slowly.C/N and pH are the whether basic indexs of slaking of reflection compost maturing process and compost, when the C/N in compost drops to below 20 and when pH is down to subacidity from initial acid-basicity, compost is fermentation maturity.Very obvious with the decline of microbial inoculum processing, compost maturity speed is fast, and in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 30d.In addition, adopt radish seed, in the compost extraction liquid of 5% interpolation decomposing agent of the present invention, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 89%, and then definite compost maturity.Further, the compost color that decomposing agent processing of the present invention ferments is chocolate; Material form is profile and collapses, evenly tiny; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste; Water ratio, is drying regime, and hand does not become piece.
2) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 11;
Table 11 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 16 58 68 70 71 71
Blank -8 -5 0 8 22 43 44
The processing heap leavening temperature that adds as can be seen from Table 11 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 94%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.
3) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 12;
Table 12 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 14 58 67 71 68 68
Blank -8 -6 0 8 23 40 40
The processing heap leavening temperature that adds as can be seen from Table 12 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 94%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.
Embodiment 5
A kind of organic matter decomposing inoculant, it is formulated according to following weight by each component: genus bacillus 53g, yeast 4g, aspergillus 8g, thermophilic mould 17g, thermophilic actinomycete 13g, high temperature fiber element decomposing bacteria 28g.
Described genus bacillus is one or several in subtilis, Bacillus licheniformis, subtilis, bacillus megaterium, bacillus cereus.
Described yeast is the one in Candida utilis bacterium, Endomycopsis Fibnligera, saccharomyces cerevisiae.
Described aspergillus is the one in aspergillus niger, aspergillus oryzae.
Described thermophilic actinomycete is the one in thermostreptomyces.
Described thermophilic mould is the one in chaetomium thermophilum, sporotrichum thermophile.
The preparation method of described organic matter decomposing inoculant, this preparation method comprises the steps:
The preparation of step 1, bacterial classification:
1) cultivation of genus bacillus: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 30-40 DEG C, cultivate 3 days, after testing, be greater than 1,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
2) cultivation of high temperature fiber element decomposing bacteria: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 50-60 DEG C, cultivate 2 days, after testing, be greater than 3,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
3) cultivation of thermophilic actinomycete: thermophilic actinomycete is seeded on solid multiplication substratum with the inoculum size of 5-15%, the temperature that controls environment 50-65 DEG C, cultivate 3-5 days, after testing, be greater than 1,000,000,000/g to thermophilic actinomycete content, described solid multiplication substratum is to be made up according to following weight percent of each component: sawdust 30%, wheat bran 54%, Semen Maydis powder 5%, dregs of beans 10%, magnesium sulfate 0.8%, sodium hydroxide 0.2%;
4) cultivation of thermophilic mould: thermophilic mould is seeded in to water ratio taking the form of liquid seeds in the solid-state fermentation culture medium of 50-60% according to the inoculum size of 2-10%, at 45-55 DEG C, cultivate 3 days, after testing, extremely thermophilic mould bacterial content is greater than 1,000,000,000/g, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 40%, wheat bran 40%, dregs of beans 20%;
5) cultivation of aspergillus: aspergillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 25-35 DEG C, cultivate 4-7 days, after testing, be greater than 500,000,000/g to aspergillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
6) saccharomycetic cultivation: yeast is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 28-35 DEG C, cultivate 3-7 days, after testing, be greater than 200,000,000/g to the mould content of yeast, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
Step 2, drying treatment: genus bacillus that step 1 is obtained, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material be in the seasoning of airing field room temperature, be down to below 10% to water ratio;
Step 3, pulverize and sieve: dry step 2 genus bacillus obtaining, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material were pulverized to 60 mesh sieves, and extracting screen underflow, is former bacterium powder;
Step 4, mix: step 3 is pulverized and sieved to the genus bacillus that obtains, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic former bacterium powder and mix and make organic matter decomposing inoculant according to following weight: genus bacillus 53g, yeast 4g, aspergillus 8g, thermophilic mould 17g, thermophilic actinomycete 13g, high temperature fiber element decomposing bacteria 28g.
The use of organism decomposing agent of the present invention:
1) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse cow dung per ton the consumption that weight percent is 0.1% Solid Organic refuse cow dung processed, and with do not throw in microbial inoculum Solid Organic refuse cow dung processing naturally in contrast.First cow dung and mushroom residue are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, carbon-nitrogen ratio 30, pH=7.2, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 13;
Table 13 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (15 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention 13 64 67 68 69 67 67
Blank 12 25 44 48 53 50 50
Add as can be seen from Table 13 the processing heap leavening temperature of decomposing agent of the present invention and pile apparently higher than blank, under certain condition, leavening temperature remains at more than 60 DEG C, has reached the requirement of high temperature high-rate composting fermentation.China's decontamination of human excreta hygienic standard (GB7959-1987) regulation, the highest compost temperature reaches 50-55 DEG C and lasting 5-7 days, and compost reaches innoxious standard.And blank microbial activities is not obvious, leavening temperature changes with envrionment temperature substantially, heats up slowly and can not reach a high temperature, and is difficult to make cattle manure slaking.In compost, find, prior fermentation temperature is rapid ascendant trend, then be generally slow decreasing trend, show under microbial inoculum effect, in cow dung, most labile organism can be decomposed at short notice, by turning, within 20 days subsequently left and right time, present Secondary Fermentation feature, still undecomposed organism further decomposes.This fermentation maturity phenomenon and rule, basically identical with the cow dung During High-Temperature Composting fermentative processing of adding external similar microbial inoculum.
Meanwhile, the smells such as the normal foul smelling of cow dung, mainly comprise that voltaile fatty acid, alcohols, phenols, aldehydes, thio-alcohol etc. more than 90 plant material.Deodorizing is one of requirement of compost treatment, in the present embodiment, cow dung after decomposing microbial inoculum compost treatment, odorless substantially.From change of soil water content, processing heap is because heap temperature is high, and moisture evaporation is fast, will be obvious under water content, and contrast heap temperature is low, moisture volatilizees naturally slowly.C/N and pH are the whether basic indexs of slaking of reflection compost maturing process and compost, when the C/N in compost drops to below 20 and when pH is down to subacidity from initial acid-basicity, compost is fermentation maturity.Very obvious with the decline of microbial inoculum processing, compost maturity speed is fast, and in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 30d.In addition, adopt radish seed, in the compost extraction liquid of 5% interpolation decomposing agent of the present invention, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 86%, and then definite compost maturity.Further, the compost color that decomposing agent processing of the present invention ferments is chocolate; Material form is profile and collapses, evenly tiny; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste; Water ratio, is drying regime, and hand does not become piece.
2) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 14;
Table 14 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 15 57 67 69 70 70
Blank -8 -4 0 9 23 42 43
The processing heap leavening temperature that adds as can be seen from Table 14 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 91%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.
3) by the organic matter decomposing inoculant of above-mentioned preparation, throw according to Solid Organic refuse pig manure per ton the consumption that weight percent is 0.1% Solid Organic refuse chicken manure processed, and with do not throw in microbial inoculum Solid Organic refuse chicken manure processing naturally in contrast.First chicken manure and straw powder are mixed according to 70% and 30% weight percent, and be adjusted to moisture 60%, make organic waste raw material, then after the half of the organic waste raw material making being mixed with decomposing microbial inoculum of the present invention, being bar buttress shape banks up, second half of the organic waste raw material making placed fermentation naturally, ferment under uniform temp condition respectively at same time, the temperature in actual measurement material.In compost process, record temperature every day and change with heap temperature, according to the suitable turning of difference intensification situation to maintain good fermentation state.Measuring result is as table 15;
Table 15 adds 7 days intensification situations of the organic matter raw material of decomposing agent of the present invention and the organic matter raw material of blank in process and changes (wherein unit be DEG C) for banking up
Envrionment temperature (8 DEG C) 1 day 2 days 3 days 4 days 5 days 6 days 7 days
Add decomposing agent of the present invention -8 14 59 68 70 69 69
Blank -8 -5 1 9 24 41 41
The processing heap leavening temperature that adds as can be seen from Table 15 decomposing agent of the present invention is obviously better than blank heap, and heat-up rate is fast, maintains high-temperature time long, and control group heats up slowly, rises less than high temperature.Fast by the compost maturity speed of microbial inoculum processing, in 18 days, C/N drops to below 20, and pH also drops to 6.5-7.0 scope because a large amount of organic acids in compost matter produce, and shows compost slaking substantially.And these two of blanks all belong to slow decreasing trend, compost maturity speed is slow, does not also reach becoming thoroughly decomposed primary standard during to 40 days.Adopt radish seed, in the compost extraction liquid of 5% organism decomposing agent of the present invention processing, in 20 DEG C of constant temperature culture 3 days, percentage of germination reached 91%, can think compost maturity.The compost color that organism decomposing agent of the present invention processing ferments is chocolate; Foul smell aspect, does not have fecaluria smelly, has compost fermentation taste.

Claims (7)

1. an organic matter decomposing inoculant, is characterized in that: it is to be made up according to following weight proportion of each component: genus bacillus 35-55 part, yeast 2-5 part, aspergillus 5-10 part, thermophilic mould 4-20 part, thermophilic actinomycete 8-15 part, high temperature fiber element decomposing bacteria 20-30 part.
2. organic matter decomposing inoculant as claimed in claim 1, is characterized in that: described genus bacillus is one or several in subtilis, Bacillus licheniformis, subtilis, bacillus megaterium, bacillus cereus.
3. organic matter decomposing inoculant as claimed in claim 1, is characterized in that: described yeast is the one in Candida utilis bacterium, Endomycopsis Fibnligera, saccharomyces cerevisiae.
4. organic matter decomposing inoculant as claimed in claim 1, is characterized in that: described aspergillus is the one in aspergillus niger, aspergillus oryzae.
5. organic matter decomposing inoculant as claimed in claim 1, is characterized in that: described thermophilic actinomycete is the one in thermostreptomyces.
6. organic matter decomposing inoculant as claimed in claim 1, is characterized in that: described thermophilic mould is the one in chaetomium thermophilum, sporotrichum thermophile.
7. a preparation method for organic matter decomposing inoculant as claimed in claim 1, is characterized in that: this preparation method comprises the steps:
The preparation of step 1, bacterial classification:
1) cultivation of genus bacillus: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 30-40 DEG C, cultivate 3 days, after testing, be greater than 1,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
2) cultivation of high temperature fiber element decomposing bacteria: genus bacillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 1-10%, the temperature that controls environment 50-60 DEG C, cultivate 2 days, after testing, be greater than 3,000,000,000/g to genus bacillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 28%, dregs of beans 10%, wheat bran 60%, ammonium sulfate 1.5%, dipotassium hydrogen phosphate 0.3%, sodium hydroxide 0.2%;
3) cultivation of thermophilic actinomycete: thermophilic actinomycete is seeded on solid multiplication substratum with the inoculum size of 5-15%, the temperature that controls environment 50-65 DEG C, cultivate 3-5 days, after testing, be greater than 1,000,000,000/g to thermophilic actinomycete content, described solid multiplication substratum is to be made up according to following weight percent of each component: sawdust 30%, wheat bran 54%, Semen Maydis powder 5%, dregs of beans 10%, magnesium sulfate 0.8%, sodium hydroxide 0.2%;
4) cultivation of thermophilic mould: thermophilic mould is seeded in to water ratio taking the form of liquid seeds in the solid-state fermentation culture medium of 50-60% according to the inoculum size of 2-10%, at 45-55 DEG C, cultivate 3 days, after testing, extremely thermophilic mould bacterial content is greater than 1,000,000,000/g, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: sawdust 40%, wheat bran 40%, dregs of beans 20%;
5) cultivation of aspergillus: aspergillus is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 25-35 DEG C, cultivate 4-7 days, after testing, be greater than 500,000,000/g to aspergillus content, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
6) saccharomycetic cultivation: yeast is seeded in to water ratio in the solid-state fermentation culture medium of 50-60% taking the inoculum size of 4-10%, the temperature that controls environment 28-35 DEG C, cultivate 3-7 days, after testing, be greater than 200,000,000/g to the mould content of yeast, described solid-state fermentation culture medium is to be made up according to following weight percent of each component: wheat bran 60%, dregs of beans 10%, Semen Maydis powder 20%, rice husk 10%;
Step 2, drying treatment: genus bacillus that step 1 is obtained, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material be in the seasoning of airing field room temperature, be down to below 10% to water ratio;
Step 3, pulverize and sieve: dry step 2 genus bacillus obtaining, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic culture material were pulverized to 60 mesh sieves, and extracting screen underflow, is former bacterium powder;
Step 4, mix: step 3 is pulverized and sieved to the genus bacillus that obtains, high temperature fiber element decomposing bacteria, thermophilic actinomycete, thermophilic mould, aspergillus and saccharomycetic former bacterium powder and mix and make organic matter decomposing inoculant according to following weight proportion: genus bacillus 35-55 part, yeast 2-5 part, aspergillus 5-10 part, thermophilic mould 4-20 part, thermophilic actinomycete 8-15 part, high temperature fiber element decomposing bacteria 20-30 part.
CN201310004556.3A 2013-01-07 2013-01-07 Organic material decomposing agent and its preparation method Pending CN103911310A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310004556.3A CN103911310A (en) 2013-01-07 2013-01-07 Organic material decomposing agent and its preparation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310004556.3A CN103911310A (en) 2013-01-07 2013-01-07 Organic material decomposing agent and its preparation method

Publications (1)

Publication Number Publication Date
CN103911310A true CN103911310A (en) 2014-07-09

Family

ID=51037318

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310004556.3A Pending CN103911310A (en) 2013-01-07 2013-01-07 Organic material decomposing agent and its preparation method

Country Status (1)

Country Link
CN (1) CN103911310A (en)

Cited By (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104692961A (en) * 2015-02-03 2015-06-10 新疆天物生态科技股份有限公司 Microorganism organic material decomposition agent as well as preparation method and applications thereof
CN104725087A (en) * 2015-01-30 2015-06-24 上海胜维有机肥有限公司 Environment-friendly and harmless novel technology for producing organic fertilizers by utilizing agricultural wastes
CN104877920A (en) * 2015-05-18 2015-09-02 中国农业科学院饲料研究所 Candida drusei LSA and application thereof in ammonia-nitrogen degradation
CN105154373A (en) * 2015-10-08 2015-12-16 山东大学 High-temperature cow dung decomposition agent and preparation method thereof
CN105272395A (en) * 2015-11-02 2016-01-27 仲恺农业工程学院 Compound microbiological preparation for quickly degrading cassava residues, and preparation method and application thereof
CN105439776A (en) * 2015-12-30 2016-03-30 广东佳居乐厨房科技有限公司 Fermentation treatment method for kitchen residues
CN106034936A (en) * 2016-06-22 2016-10-26 广西横县莉妃花圃农业科技有限公司 Magnolia denudate pot cultivation method
CN106069050A (en) * 2016-06-22 2016-11-09 广西横县莉妃花圃农业科技有限公司 The breeding method that a kind of Flos Gardeniae is potted plant
CN106927971A (en) * 2017-02-23 2017-07-07 北京绿安创华环保科技股份有限公司 A kind of sludge soil-repairing agent, preparation method and its application in sludge is dissolved
CN107721490A (en) * 2017-10-31 2018-02-23 贵州沿河深山老林农业科技发展有限公司 A kind of citrus nursery Nutrition Soil and preparation method thereof
CN108147868A (en) * 2017-12-27 2018-06-12 厦门理工学院 A kind of method, bamboo fermentate and its application of the decomposed fermentation of bamboo
CN108997034A (en) * 2018-08-10 2018-12-14 成都惠森生物技术有限公司 Decomposed starting agent of one kind and preparation method thereof
CN109679857A (en) * 2018-12-25 2019-04-26 广东高龙环保科技有限公司 A kind of compost maturity microbial inoculum
CN109721386A (en) * 2019-03-11 2019-05-07 广州绿芽生态科技有限责任公司 A kind of feces of livestock and poultry organic fertilizer fermentation agent, feces of livestock and poultry organic fertilizer and the organic fertilizer odorlessization manufacturing process
CN110156498A (en) * 2019-04-30 2019-08-23 江苏思威博生物科技有限公司 A kind of feces of livestock and poultry high temperature desiccation microbial inoculum and its application method
CN110204392A (en) * 2019-07-05 2019-09-06 鹤壁市人元生物技术发展有限公司 A kind of oil plant dregs of rice fermented liquid foliar fertilizer and preparation method thereof
CN110240499A (en) * 2019-06-26 2019-09-17 江西省蚕桑茶叶研究所(江西省经济作物研究所) A kind of method of ramulus mori Rapid Fermentation production organic fertilizer
CN110903113A (en) * 2019-12-31 2020-03-24 河南农业大学 Organic fertilizer composite bacteria starter, special equipment thereof and application of starter in fermentation before fast maturity of organic fertilizer
CN112048449A (en) * 2020-07-31 2020-12-08 南京农业大学 Compost composite microbial inoculum and application thereof
CN112174747A (en) * 2020-09-30 2021-01-05 广东石油化工学院 Method for preparing organic fertilizer by combining pig farm wastewater MAP (MAP) precipitated sludge and banana stems and leaves
CN112501030A (en) * 2020-11-30 2021-03-16 九江礼涞生物科技有限公司 Application of livestock and poultry manure leavening agent product
CN112662600A (en) * 2021-01-28 2021-04-16 福建省微生物研究所 Microecological preparation for high-temperature decomposition and deodorization of chicken manure and preparation method thereof
CN112725239A (en) * 2021-02-05 2021-04-30 宁夏大学 Decomposing inoculant with cellulose degradation and deodorization functions as well as preparation method and application thereof
CN113249271A (en) * 2021-06-28 2021-08-13 丰德生物技术(大连)有限公司 Special compound soil improvement microbial agent for rice
CN113548916A (en) * 2021-06-25 2021-10-26 山东农大肥业科技有限公司 Special decomposition agent containing oxygenation agent and humic acid for cage-rearing chicken manure and application thereof
CN114946592A (en) * 2022-06-25 2022-08-30 上海丰麓园林绿化建设有限公司 Composite nutrient soil and preparation method and application thereof
CN114946600A (en) * 2022-07-14 2022-08-30 广西壮族自治区亚热带作物研究所(广西亚热带农产品加工研究所) Vegetable seedling raising substrate and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102351589A (en) * 2011-07-14 2012-02-15 李桓宇 Bio-organic fertilizer produced by use of municipal sludge and production method thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102351589A (en) * 2011-07-14 2012-02-15 李桓宇 Bio-organic fertilizer produced by use of municipal sludge and production method thereof

Cited By (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104725087A (en) * 2015-01-30 2015-06-24 上海胜维有机肥有限公司 Environment-friendly and harmless novel technology for producing organic fertilizers by utilizing agricultural wastes
CN104692961A (en) * 2015-02-03 2015-06-10 新疆天物生态科技股份有限公司 Microorganism organic material decomposition agent as well as preparation method and applications thereof
CN104877920B (en) * 2015-05-18 2018-05-25 中国农业科学院饲料研究所 Candida krusei LSA and its application in degradation of ammonia nitrogen
CN104877920A (en) * 2015-05-18 2015-09-02 中国农业科学院饲料研究所 Candida drusei LSA and application thereof in ammonia-nitrogen degradation
CN105154373A (en) * 2015-10-08 2015-12-16 山东大学 High-temperature cow dung decomposition agent and preparation method thereof
CN105272395A (en) * 2015-11-02 2016-01-27 仲恺农业工程学院 Compound microbiological preparation for quickly degrading cassava residues, and preparation method and application thereof
CN105272395B (en) * 2015-11-02 2019-04-05 仲恺农业工程学院 A kind of complex microorganism preparations and the preparation method and application thereof for manioc waste fast degradation
CN105439776A (en) * 2015-12-30 2016-03-30 广东佳居乐厨房科技有限公司 Fermentation treatment method for kitchen residues
CN106034936A (en) * 2016-06-22 2016-10-26 广西横县莉妃花圃农业科技有限公司 Magnolia denudate pot cultivation method
CN106069050A (en) * 2016-06-22 2016-11-09 广西横县莉妃花圃农业科技有限公司 The breeding method that a kind of Flos Gardeniae is potted plant
CN106927971A (en) * 2017-02-23 2017-07-07 北京绿安创华环保科技股份有限公司 A kind of sludge soil-repairing agent, preparation method and its application in sludge is dissolved
CN107721490A (en) * 2017-10-31 2018-02-23 贵州沿河深山老林农业科技发展有限公司 A kind of citrus nursery Nutrition Soil and preparation method thereof
CN108147868A (en) * 2017-12-27 2018-06-12 厦门理工学院 A kind of method, bamboo fermentate and its application of the decomposed fermentation of bamboo
CN108997034A (en) * 2018-08-10 2018-12-14 成都惠森生物技术有限公司 Decomposed starting agent of one kind and preparation method thereof
CN109679857A (en) * 2018-12-25 2019-04-26 广东高龙环保科技有限公司 A kind of compost maturity microbial inoculum
CN109721386A (en) * 2019-03-11 2019-05-07 广州绿芽生态科技有限责任公司 A kind of feces of livestock and poultry organic fertilizer fermentation agent, feces of livestock and poultry organic fertilizer and the organic fertilizer odorlessization manufacturing process
CN110156498A (en) * 2019-04-30 2019-08-23 江苏思威博生物科技有限公司 A kind of feces of livestock and poultry high temperature desiccation microbial inoculum and its application method
CN110240499A (en) * 2019-06-26 2019-09-17 江西省蚕桑茶叶研究所(江西省经济作物研究所) A kind of method of ramulus mori Rapid Fermentation production organic fertilizer
CN110204392A (en) * 2019-07-05 2019-09-06 鹤壁市人元生物技术发展有限公司 A kind of oil plant dregs of rice fermented liquid foliar fertilizer and preparation method thereof
CN110903113B (en) * 2019-12-31 2023-09-19 河南农业大学 Organic fertilizer composite bacteria starter, special equipment thereof and application thereof in organic fertilizer fast-maturing pre-fermentation
CN110903113A (en) * 2019-12-31 2020-03-24 河南农业大学 Organic fertilizer composite bacteria starter, special equipment thereof and application of starter in fermentation before fast maturity of organic fertilizer
CN112048449A (en) * 2020-07-31 2020-12-08 南京农业大学 Compost composite microbial inoculum and application thereof
CN112174747A (en) * 2020-09-30 2021-01-05 广东石油化工学院 Method for preparing organic fertilizer by combining pig farm wastewater MAP (MAP) precipitated sludge and banana stems and leaves
CN112501030A (en) * 2020-11-30 2021-03-16 九江礼涞生物科技有限公司 Application of livestock and poultry manure leavening agent product
CN112662600A (en) * 2021-01-28 2021-04-16 福建省微生物研究所 Microecological preparation for high-temperature decomposition and deodorization of chicken manure and preparation method thereof
CN112725239A (en) * 2021-02-05 2021-04-30 宁夏大学 Decomposing inoculant with cellulose degradation and deodorization functions as well as preparation method and application thereof
CN113548916A (en) * 2021-06-25 2021-10-26 山东农大肥业科技有限公司 Special decomposition agent containing oxygenation agent and humic acid for cage-rearing chicken manure and application thereof
CN113249271A (en) * 2021-06-28 2021-08-13 丰德生物技术(大连)有限公司 Special compound soil improvement microbial agent for rice
CN114946592A (en) * 2022-06-25 2022-08-30 上海丰麓园林绿化建设有限公司 Composite nutrient soil and preparation method and application thereof
CN114946600A (en) * 2022-07-14 2022-08-30 广西壮族自治区亚热带作物研究所(广西亚热带农产品加工研究所) Vegetable seedling raising substrate and preparation method thereof

Similar Documents

Publication Publication Date Title
CN103911310A (en) Organic material decomposing agent and its preparation method
CN101186879B (en) Agriculture castoff compost ternary microorganism composite microbial agent
CN103053432B (en) Fermenting bed bioactive padding and preparation and application methods thereof
US8778048B2 (en) Biochemical humic acid product prepared from kitchen waste and the method of preparing the same
CN104293694B (en) A kind of preparation method of sludge aerobic compost composite bacteria agent
CN101186537B (en) Echelon circulation inoculation temperature-control compost method
CN102584364B (en) Biologic organic fertilizer and preparation method thereof
CN100425690C (en) Fast harmless biological treating process of animal excrement and manure water
CN101704691B (en) Bamboo residue organic fertilizer and method for preparing same
CN104232551A (en) Pig-chicken excrement fermenting compound bacteria, preparation method and method of utilizing compound bacteria to ferment and prepare organic fertilizer
CN105218254A (en) A kind of Microbial and ecological organic fertilizers material and preparation method thereof
CN105010154B (en) The technique that a kind of recycling sheep manure prepares fermenting bed padding
CN105949006A (en) Compound fertilizer taking cow dung as main material and production method
CN104829309A (en) Environmental-protection organic fertilizer and production method thereof
CN109182228A (en) A kind of complex micro organism fungicide and preparation method thereof for feces of livestock and poultry and straw under high temperature composting organic fertilizer material
CN106748533A (en) A kind of cattle manure biological organic fertilizer and preparation method thereof
CN104261919A (en) Process for producing efficient bioorganic fertilizers by resource utilization of dairy cow manure
CN106478167A (en) Organic fermentation fertilizer and preparation method thereof
CN104255538A (en) Technology for preparing fermentation bed padding by recycling livestock excrements and crop straws
CN106479937A (en) A kind of purposes of the organic matter decomposing inoculant with deodorant, getting fat function and preparation method thereof with it
CN104862298A (en) Composite microbial culture starter and preparation method thereof
CN106946630A (en) A kind of organic fungi-manure using sludge as raw material and preparation method thereof
CN109180236A (en) A kind of new process of aerobic fermentation processing chicken manure
CN104312947A (en) Multi-strain efficient straw-decomposing inoculant and preparation method and application thereof
CN104293711A (en) Sheep-and-cow dung fermented compound bacteria, preparation method thereof and method for preparing organic fertilizer by utilizing fermentation of compound bacteria

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20140709