CN115181706A - Microbial agent for livestock and poultry manure compost and preparation method thereof - Google Patents
Microbial agent for livestock and poultry manure compost and preparation method thereof Download PDFInfo
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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Abstract
The invention discloses a microbial agent for livestock and poultry manure compost and a preparation method thereof, relating to the technical field of microbial agents and comprising the following components: the microbial inoculum is used for improving the viability in the soil environment, transforming nitrogen and potassium in soil, improving the soil fertility and stimulating the growth of crops, reducing the using amount of chemical fertilizers, inhibiting and killing the reproduction of germs, achieving the effects of increasing yield and income, inhibiting diseases, improving the quality, integrating three effects into one, reducing the input cost, simultaneously achieving the control efficiency on various nematodes, improving the immunity of the crops and strengthening the disease resistance of the crops.
Description
Technical Field
The invention relates to the technical field of microbial agents, in particular to a microbial agent for livestock and poultry manure composting and a preparation method thereof.
Background
The existing microbial agent for livestock and poultry manure compost is relatively complex in preparation steps and relatively complex in operation, not only is the investment cost increased, but also the prepared finished product is relatively weak in sterilization and bacteriostasis effect, relatively poor in viability in the soil environment and incapable of better helping crops to grow, so that workers in the field provide the microbial agent for livestock and poultry manure compost and the preparation method thereof.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a microbial agent for livestock and poultry manure compost and a preparation method thereof, and solves the problems that the existing microbial agent for livestock and poultry manure compost is high in investment cost, and the prepared finished product is weak in sterilization and bacteriostasis effects, poor in viability in soil environment and incapable of better helping the growth of crops.
In order to achieve the purpose, the invention is realized by the following technical scheme: a microbial agent for livestock and poultry manure compost comprises the following components: bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, organophosphorus bacteria, potassium bacteria and Rhizobium astrophilum;
the microbial agent for the livestock and poultry manure compost is prepared from the following components in parts by mass: 2.0-3.0 parts of bacillus subtilis, 2.6-3.7 parts of azotobacter vinelandii, 1.8-2.4 parts of paecilomyces lilacinus, 3.3-4.0 parts of bacillus coagulans, 2.1-3.1 parts of trichoderma harzianum, 4.0-4.2 parts of streptomyces microflavus, 0.6-0.8 part of trichoderma, 0.3-0.4 part of organophosphorus bacteria, 0.3-0.4 part of potassium bacteria and 0.5-1.0 part of rhizobium sinense.
The invention further adopts the technical scheme that the medicament is prepared from the following components in parts by mass: 2.0 parts of bacillus subtilis, 2.6 parts of azotobacter vinelandii, 1.8 parts of paecilomyces lilacinus, 3.3 parts of bacillus coagulans, 2.1 parts of trichoderma harzianum, 4.0 parts of streptomyces microflavus, 0.6 part of trichoderma, 0.3 part of organophosphorus bacteria, 0.3 part of potassium bacteria and 0.5 part of rhizobium purpureum.
The invention further adopts the technical scheme that the medicament is prepared from the following components in parts by mass: 2.5 parts of bacillus subtilis, 3.0 parts of azotobacter vinelandii, 2.1 parts of paecilomyces lilacinus, 3.6 parts of bacillus coagulans, 2.6 parts of trichoderma harzianum, 4.1 parts of streptomyces microflavus, 0.7 part of trichoderma, 0.35 part of organophosphorus bacteria, 0.35 part of potassium bacteria and 0.7 part of rhizobium purpureum.
The invention further adopts the technical scheme that the medicament is prepared from the following components in parts by mass: 3.0 parts of bacillus subtilis, 3.7 parts of azotobacter vinelandii, 2.4 parts of paecilomyces lilacinus, 4.0 parts of bacillus coagulans, 3.1 parts of trichoderma harzianum, 4.2 parts of streptomyces microflavus, 0.8 part of trichoderma, 0.4 part of organophosphorus bacteria, 0.4 part of potassium bacteria and 1.0 part of rhizobium purpureum.
As a further technical scheme of the invention, the streptomyces microflavus is mainly prepared by fermenting soybean cake powder, glucose, calcium hydrochloride and a liquid culture medium of sodium chloride.
As a further technical scheme of the invention, the soybean cake powder comprises 3g of glucose, 0.5g of calcium hydrochloride and 0.4g of sodium chloride.
As a further technical scheme, the azotobacter vinelandii and the potassium bacteria are both cultured by adopting an Ashby culture medium, the trichoderma is cultured by adopting a potato sucrose culture medium, the organophosphorus bacteria is cultured by adopting a lecithin culture medium, and the rhizobium astragalis is cultured by adopting a yeast juice sucrose culture medium.
The preparation method of the microbial agent for the livestock and poultry manure compost comprises the following steps:
s1, strain culture: respectively inoculating bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, organophosphorus bacteria, potassium bacteria and rhizobium astrophilum into respective culture media for culture;
s2, preparing a bacterial liquid; putting the various strains cultured in the step S1 into a medicine bottle of a seed culture medium in a slant inoculation mode, and performing individual culture by using a liquid culture medium to obtain a bacterial liquid of the strains;
s3, mixing bacterial liquid; uniformly putting the various bacterial liquids prepared in the step S2 into a fermentation tank filled with seeds, and stirring and mixing to obtain a composite seed liquid;
s4, preparing a composite bacterial liquid; inoculating all the seed liquid in the step S3 into a seed tank filled with a fermentation culture medium for fermentation culture to obtain a compound bacterial liquid;
s5, preparing a biological agent: drying and spraying the compound bacterial liquid obtained in the step S4 to obtain a finished product biological agent;
s6, packaging and warehousing: and (5) adding the finished product biological agent in the step (S5) into a filling machine, packaging the finished product biological agent into a container by using the filling machine, and storing the packaged finished product biological agent in a warehouse.
Advantageous effects
The invention provides a microbial agent for livestock and poultry manure compost and a preparation method thereof. Compared with the prior art, the method has the following beneficial effects:
1. the microbial agent for the compost of livestock and poultry manure and the preparation method thereof have the advantages that by using the microbial agent, the survival ability in the soil environment is improved, nitrogen and potassium in the soil are converted, the soil fertility is improved, the growth of crops is stimulated, the using amount of a chemical fertilizer is reduced, the germ reproduction is inhibited, the effects of increasing the yield and income, inhibiting diseases, improving the quality and integrating three effects into one, and the input cost is further reduced.
2. A microbial agent for the compost of animal and fowls excrement and urine is prepared from Trichoderma harzianum able to secrete the cell wall degrading enzyme of pathogenic bacteria to destroy the cell wall of pathogenic bacteria, resulting in killing pathogenic bacteria, and Paecilomyces lilacinus with the function of preventing and treating more nematodes.
Drawings
FIG. 1 is a flow chart of the preparation method of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The invention provides a technical scheme that: a microbial agent for livestock and poultry manure compost is prepared from the following components in parts by mass: 2.0 parts of bacillus subtilis, 2.6 parts of azotobacter vinelandii, 1.8 parts of paecilomyces lilacinus, 3.3 parts of bacillus coagulans, 2.1 parts of trichoderma harzianum, 4.0 parts of streptomyces microflavus, 0.6 part of trichoderma, 0.3 part of organophosphorus bacteria, 0.3 part of potassium bacteria and 0.5 part of rhizobium purpureum.
The streptomyces microflavus is mainly prepared by fermenting soybean cake powder, glucose, calcium hydrochloride and a liquid culture medium of sodium chloride.
The soybean cake powder content is 3g, the glucose content is 3g, the calcium hydrochloride content is 0.5g, and the sodium chloride content is 0.4g.
The azotobacter vinelandii and the potassium bacteria are cultured by adopting an Ashby culture medium, the trichoderma is cultured by adopting a potato sucrose culture medium, the organophosphorus bacteria is cultured by adopting a lecithin culture medium, and the astragalus root nodule bacteria is cultured by adopting a yeast juice sucrose culture medium.
The preparation method of the microbial agent for the livestock and poultry manure compost comprises the following steps:
s1, strain culture: respectively inoculating bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, organophosphorus bacteria, potassium bacteria and rhizobium astragalii into respective culture medium for culture;
s2, preparing a bacterial liquid; putting the various strains cultured in the step S1 into a medicine bottle of a seed culture medium in a slant inoculation mode, and performing individual culture by using a liquid culture medium to obtain a bacterial liquid of the strains;
s3, mixing bacterial liquid; uniformly putting the various bacterial liquids prepared in the step S2 into a fermentation tank filled with seeds, and stirring and mixing to obtain a composite seed liquid;
s4, preparing a composite bacterial liquid; inoculating all the seed liquid in the step S3 into a seed tank filled with a fermentation culture medium for fermentation culture to obtain a compound bacterial liquid;
s5, preparing a biological agent: drying and spraying the compound bacterial liquid obtained in the step S4 to obtain a finished product biological agent;
s6, packaging and warehousing: and (5) adding the finished product biological agent in the step S5 into a filling machine, packaging the finished product biological agent into a container by using the filling machine, and storing the packaged finished product biological agent in a warehouse.
Example 2
The invention provides a technical scheme that: a microbial agent for livestock and poultry manure compost is prepared from the following components in parts by mass: 2.5 parts of bacillus subtilis, 3.0 parts of azotobacter vinelandii, 2.1 parts of paecilomyces lilacinus, 3.6 parts of bacillus coagulans, 2.6 parts of trichoderma harzianum, 4.1 parts of streptomyces microflavus, 0.7 part of trichoderma, 0.35 part of organophosphorus bacteria, 0.35 part of potassium bacteria and 0.7 part of rhizobium purpureum.
The streptomyces microflavus is mainly prepared by fermenting soybean cake powder, glucose, calcium hydrochloride and a liquid culture medium of sodium chloride.
The soybean cake powder content is 3g, the glucose content is 3g, the calcium hydrochloride content is 0.5g, and the sodium chloride content is 0.4g.
The azotobacter vinelandii and the potassium bacteria are cultured by adopting an Ashby culture medium, the trichoderma is cultured by adopting a potato sucrose culture medium, the organophosphorus bacteria is cultured by adopting a lecithin culture medium, and the astragalus root nodule bacteria is cultured by adopting a yeast juice sucrose culture medium.
The preparation method of the microbial agent for the livestock manure compost comprises the following steps:
s1, strain culture: respectively inoculating bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, organophosphorus bacteria, potassium bacteria and rhizobium astragalii into respective culture medium for culture;
s2, preparing bacterial liquid; putting the various strains cultured in the step S1 into a medicine bottle of a seed culture medium in a slant inoculation mode, and performing individual culture by using a liquid culture medium to obtain a bacterial liquid of the strains;
s3, mixing bacterial liquid; uniformly putting the various bacterial liquids prepared in the step S2 into a fermentation tank filled with seeds, and stirring and mixing to obtain a composite seed liquid;
s4, preparing a composite bacterial liquid; inoculating all the seed liquid in the step S3 into a seed tank filled with a fermentation culture medium for fermentation culture to obtain a compound bacterial liquid;
s5, preparing a biological agent: drying and spraying the compound bacterial liquid obtained in the step S4 to obtain a finished product biological agent;
s6, packaging and warehousing: and (5) adding the finished product biological agent in the step S5 into a filling machine, packaging the finished product biological agent into a container by using the filling machine, and storing the packaged finished product biological agent in a warehouse.
Example 3
The invention provides a technical scheme that: a microbial agent for livestock and poultry manure compost is prepared from the following components in parts by mass: 3.0 parts of bacillus subtilis, 3.7 parts of azotobacter vinelandii, 2.4 parts of paecilomyces lilacinus, 4.0 parts of bacillus coagulans, 3.1 parts of trichoderma harzianum, 4.2 parts of streptomyces microflavus, 0.8 part of trichoderma, 0.4 part of organophosphorus bacteria, 0.4 part of potassium bacteria and 1.0 part of rhizobium purpureum.
The streptomyces microflavus is mainly prepared by fermenting soybean cake powder, glucose, calcium hydrochloride and a liquid culture medium of sodium chloride.
The soybean cake powder content is 3g, the glucose content is 3g, the calcium hydrochloride content is 0.5g, and the sodium chloride content is 0.4g.
The azotobacter vinelandii and potassium bacteria are cultured by adopting an Ashby culture medium, the trichoderma is cultured by adopting a potato sucrose culture medium, the organophosphorus bacteria is cultured by adopting a lecithin culture medium, and the astragalus root nodule bacteria is cultured by adopting a yeast juice sucrose culture medium.
The preparation method of the microbial agent for the livestock manure compost comprises the following steps:
s1, strain culture: respectively inoculating bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, organophosphorus bacteria, potassium bacteria and rhizobium astrophilum into respective culture media for culture;
s2, preparing a bacterial liquid; putting the various strains cultured in the step S1 into a medicine bottle of a seed culture medium in a slant inoculation mode, and performing individual culture by using a liquid culture medium to obtain a bacterial liquid of the strains;
s3, mixing bacterial liquid; uniformly putting the various bacterial liquids prepared in the step S2 into a fermentation tank filled with seeds, and stirring and mixing to obtain a composite seed liquid;
s4, preparing a composite bacterial liquid; inoculating all the seed liquid in the step S3 into a seed tank filled with a fermentation culture medium for fermentation culture to obtain a compound bacterial liquid;
s5, preparing a biological agent: drying and spraying the compound bacterial liquid obtained in the step S4 to obtain a finished product biological agent;
s6, packaging and warehousing: and (5) adding the finished product biological agent in the step S5 into a filling machine, packaging the finished product biological agent into a container by using the filling machine, and storing the packaged finished product biological agent in a warehouse.
1. A Huang Fangxian bacterium antagonism test method:
(1) taking brown azotobacter, organophosphorus bacteria, potassium bacteria, astragalus root nodule bacteria and trichoderma one tube respectively;
(2) respectively washing out thalli by using sterile water, sucking bacterial suspension for 1mm, uniformly mixing with an uncured agar culture medium, and pouring into a culture dish;
(3) after solidification, dipping fermentation liquor on a circular filter paper sheet with the diameter of 1cm, pasting the fermentation liquor on a culture medium, culturing for 5-7 days at 26-28 ℃, and observing the size of a bacteriostatic circle;
the results are shown in the following table:
| bacterial strains | Diameter of bacteriostatic circle (mm) |
| Azotobacter vinelandii | 0 (dense lawn 6mm wide) |
| Organophosphorus bacteria | 0 |
| Potassium bacteria | 0 |
| Root nodule bacteria of milk vetch | 0 |
| Trichoderma reesei | 17-18 |
The test results in the table show that: the fine Huang Fangxian bacteria has obvious inhibition effect on trichoderma, no inhibition effect on potassium bacteria, organophosphorus bacteria and astragalus sinicus rhizobium, no inhibition effect on azotobacter vinelandii, and 6mm thick and dense bacterial lawn of azotobacter vinelandii around a filter paper sheet, and all the bacteria have the effect of stimulating growth.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A microbial agent for livestock and poultry manure compost is characterized by comprising the following components: bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma reesei, organophosphorus bacteria, potassium bacteria, and Rhizobium astragalis;
the microbial agent for the livestock and poultry manure compost is prepared from the following components in parts by mass: 2.0-3.0 parts of bacillus subtilis, 2.6-3.7 parts of azotobacter vinelandii, 1.8-2.4 parts of paecilomyces lilacinus, 3.3-4.0 parts of bacillus coagulans, 2.1-3.1 parts of trichoderma harzianum, 4.0-4.2 parts of streptomyces microflavus, 0.6-0.8 part of trichoderma, 0.3-0.4 part of organophosphorus bacteria, 0.3-0.4 part of potassium bacteria and 0.5-1.0 part of rhizobium sinense.
2. The microbial agent for livestock and poultry manure compost according to claim 1, which is prepared from the following components in parts by mass: 2.0 parts of bacillus subtilis, 2.6 parts of azotobacter vinelandii, 1.8 parts of paecilomyces lilacinus, 3.3 parts of bacillus coagulans, 2.1 parts of trichoderma harzianum, 4.0 parts of streptomyces microflavus, 0.6 part of trichoderma, 0.3 part of organophosphorus bacteria, 0.3 part of potassium bacteria and 0.5 part of rhizobium purpureum.
3. The microbial agent for livestock and poultry manure compost according to claim 1, which is prepared from the following components in parts by mass: 2.5 parts of bacillus subtilis, 3.0 parts of azotobacter vinelandii, 2.1 parts of paecilomyces lilacinus, 3.6 parts of bacillus coagulans, 2.6 parts of trichoderma harzianum, 4.1 parts of streptomyces microflavus, 0.7 part of trichoderma, 0.35 part of organophosphorus bacteria, 0.35 part of potassium bacteria and 0.7 part of rhizobium purpureum.
4. The microbial agent for livestock and poultry manure compost as claimed in claim 1, which is prepared from the following components in parts by mass: 3.0 parts of bacillus subtilis, 3.7 parts of azotobacter vinelandii, 2.4 parts of paecilomyces lilacinus, 4.0 parts of bacillus coagulans, 3.1 parts of trichoderma harzianum, 4.2 parts of streptomyces microflavus, 0.8 part of trichoderma, 0.4 part of organophosphorus bacteria, 0.4 part of potassium bacteria and 1.0 part of rhizobium purpureum.
5. The microbial inoculant for compost of livestock and poultry manure as claimed in claim 1, wherein said streptomyces microflavus is mainly fermented from liquid culture medium of soybean cake powder, glucose, calcium hydrochloride and sodium chloride.
6. The microbial inoculant for livestock and poultry manure compost as claimed in claim 5, wherein the soybean cake powder content is 3g, the glucose content is 3g, the calcium hydrochloride content is 0.5g, and the sodium chloride content is 0.4g.
7. The microbial inoculant for composting livestock and poultry manure of claim 1, wherein said azotobacter vinelandii and said potassium bacteria are both cultured on Ashby medium, said trichoderma is cultured on potato sucrose medium, said organophosphorus bacteria is cultured on lecithin medium, and said rhizobium sinense is cultured on yeast juice sucrose medium.
8. The microbial inoculant for composting livestock and poultry manure as claimed in any one of claims 1 to 7, wherein the preparation method comprises the following steps:
s1, strain culture: respectively inoculating bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoderma harzianum, streptomyces microflavus, trichoderma, organophosphorus bacteria, potassium bacteria and rhizobium astragalii into respective culture medium for culture;
s2, preparing a bacterial liquid; putting the various strains cultured in the step S1 into a medicine bottle of a seed culture medium in a slant inoculation mode, and performing individual culture by using a liquid culture medium to obtain a bacterial liquid of the strains;
s3, mixing bacterial liquid; uniformly putting the various bacterial liquids prepared in the step S2 into a fermentation tank filled with seeds, and stirring and mixing to obtain a composite seed liquid;
s4, preparing a composite bacterial liquid; inoculating all the seed liquid in the step S3 into a seed tank filled with a fermentation culture medium for fermentation culture to obtain a compound bacterial liquid;
s5, preparing a biological agent: drying and spraying the compound bacterial liquid obtained in the step S4 to obtain a finished product biological agent;
s6, packaging and warehousing: and (5) adding the finished product biological agent in the step S5 into a filling machine, packaging the finished product biological agent into a container by using the filling machine, and storing the packaged finished product biological agent in a warehouse.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210990953.1A CN115181706A (en) | 2022-08-18 | 2022-08-18 | Microbial agent for livestock and poultry manure compost and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210990953.1A CN115181706A (en) | 2022-08-18 | 2022-08-18 | Microbial agent for livestock and poultry manure compost and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115181706A true CN115181706A (en) | 2022-10-14 |
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| CN116004246A (en) * | 2023-02-15 | 2023-04-25 | 山东君德生物科技有限公司 | Microbial farmland soil restoration agent and microbial farmland soil restoration method |
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